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      • Open Access Article

        1 - Ultrasound-Assisted Extraction of Antioxidant Extract from Lettuce (Lactuca sativa L.) Wastes and Evaluation of the Antioxidative Activity
        N. Ahmadi Kamazani A. H. Elhamirad M. Ghavami M. Moridi Farimani M. Armin
        Introduction: In spite of the high antioxidant activity in lettuce wastes, there is not any reports on the application of the antioxidant extract from lettuce wastes to stabilize the edible oils. The aims of this study were to investigate the recovery of antioxidant ext More
        Introduction: In spite of the high antioxidant activity in lettuce wastes, there is not any reports on the application of the antioxidant extract from lettuce wastes to stabilize the edible oils. The aims of this study were to investigate the recovery of antioxidant extract from outer leaves of lettuce as the wastes via ultrasonic extraction and evaluate the antioxidative effect of the extract. Materials and Methods: The powdered lettuce sample was extracted with ethanol/H2O (70:30, V/V) using ultrasonic extraction at the temperature of 50˚C and time of 30 minutes with the frequency of 40 KHZ and solid to solvent ratio of 1:20 (w/v) in an ultrasound water bath. The extractive yield, total phenolic compound (TPC) and IC50 were determined. The yielded extract was added to tallow olein in order to study its stabilizing effect. The protective effects of the extracts at different concentrations were evaluated by monitoring the peroxide, p-anisidine, totox values and oxidative stability index levels under accelerated oxidation. The antioxidant activities of the extract of the lettuce wastes at different concentrations to stabilise tallow olein were compared to BHA and BHT at 200 ppm concentration. Results: The extractive yield, TPC and IC50 were 30.45 ± 1.20 (%), 600.15 ± 6.29 (mg GAE /100g DW) and 174.05 ppm, respectively. The results indicated that the extract at 2000 ppm have a good activity as compared to BHT at 200 ppm. Conclusion: The antioxidant extract of the lettuce wastes might used as a natural antioxidant in retarding the oxidation rate of edible oils. Manuscript profile
      • Open Access Article

        2 - Evaluation of the Antioxidative Effects of Black Tea Extracts
        Z. Nazari M. Gharachorloo A. H. Elhamirad
        Introduction: Oxidative rancidity is one of the important reactions in oils and fats. Tea contains some effective antioxidants namely tannin and catechins. In this research project the antioxidative activities of tea extract in comparison with tannic acid has been inves More
        Introduction: Oxidative rancidity is one of the important reactions in oils and fats. Tea contains some effective antioxidants namely tannin and catechins. In this research project the antioxidative activities of tea extract in comparison with tannic acid has been investigated.  Materials and Methods: This study was carried out in order to compare the effects of three different extraction methods consisted of methanolic extraction, agarwal method and extraction with hot water. In order to investigate the antioxidant activities, three concentrations of tannic acid (0.01%, 0.05% and 0.1%) and tea extract (0.1%, 0.5% and 1%) were added to four types of oils with different fatty acid unsaturation and composition (sunflower, canola, olive oils and tallow) separately. The induction period was measured by Rancimat at 120ºC and peroxide values were determined after 24 hours of heating intervals at 105ºC for six consecutive days.  Results: According to the results, the highest extraction yield belonged to the hot water extraction method. The results showed that different concentrations of tea extract had antioxidative property. In sunflower and canola oils, 0.5% tea extract and 0.1% tannic acid and in tallow and olive oils, 1% tea extract and 0.1% tannic acid showed the highest activity to increase the induction period and to prevent the increase of peroxide values of oils. Olive oil containing natural antioxidants and tallow that is deficient in natural antioxidants showed increased induction periods as the concentrations of the extract were increased.  Conclusion: Tannic acid and tea extract exhibited antioxidant activities by increasing the induction periods of oils and fats while preventing the increase of peroxide values.  Manuscript profile
      • Open Access Article

        3 - Evaluation of Antioxidant and Chelating Activities of Ginger Extract
        zahra kamali rosta maryam gharachorloo amirhosein elhami rad reza azizi nezhad
        Introduction: Oxidation of oils and fats might be considered as the main factor affecting the quality of the food in general. This process might be controlled by the application of different methods and means. Antioxidants are regarded as a tool to achieve this aim. Alt More
        Introduction: Oxidation of oils and fats might be considered as the main factor affecting the quality of the food in general. This process might be controlled by the application of different methods and means. Antioxidants are regarded as a tool to achieve this aim. Although the application of synthetic antioxidants due to their possible side effects might not be desirable, the application of natural antioxidants present in various food namely spices that have been consumed by man for years might be suitable alternatives. Therefore the aim of present investigation is to understand the antioxidant and chelating activities of chemical compounds present in ginger.Materials and Methods: Ginger extracts (Zingiber officinale Rosceo) were prepared by cold extraction using acetone and methanol as extracting solvents. Total phenolic compounds were determined and the extracts were added to tallow; a substrate free of natural antioxidants at concentrations of 0.02, 0.04, 0.06, 0.08 and 0.10 %. Peroxide value and induction period measurements were employed as means to determine the antioxidant activities of the extracts were compared to TBHQ, the synthetic antioxidant. The chelating activities of the extracts were determined by the application of copper in the form of 4-cyclohexyl butyric acid copper salt followed by the determination of peroxide value and induction period of the substrate.Results: The results indicated that the antioxidant activities of the extracts are concentration dependent and as concentration is increase better activity is observed. The acetone extract at 0.1% should a good activity ever better than the addition of 0.01% TBHQ. The acetone extract also exhibited chelating activity at 0.1%.Conclusion: Both methanol and acetone extracts of ginger exhibited antioxidant and chelating activities when added to tallow a substrate free of natural antioxidants Manuscript profile
      • Open Access Article

        4 - The Chelating Effect of Black Tea Extract on Metals
        Z. Nazari M. Gharachorloo A. H. Elhamirad
        Introduction: The oxidation process in oils and fats and the speed of oxidation to someextent depends on the presence of prooxidants namely metals. The application of chelatingagents namely citric acid among others can retard this process. In this study the chelatingeff More
        Introduction: The oxidation process in oils and fats and the speed of oxidation to someextent depends on the presence of prooxidants namely metals. The application of chelatingagents namely citric acid among others can retard this process. In this study the chelatingeffect of tea extract, tannic acid and citric acid are evaluated and compared to each other.Materials and Methods: Copper in the organic form at the concentration of 0.1 ppm wereadded to tallow, sunflower seed, canola and olive oils and then by the addition of 0.01% citricacid, 0.01% tannic acid and 0.1% tea extract the effect of chelation concerned with each oilhas been evaluated.Results: The results indicated that all the chemicals (citric and tannic acids) and tea extractadded have chelating effect on copper and increased the induction period of the oilsconcerned.Conclusion: It was concluded that tannic acid followed by tea extract and citric acid had thebest chelating effect on copper. Manuscript profile