-
Open Access Article
1 - Antibiotic resistance in patients with diabetic foot ulcers
Shima ShantiaeeDiabetes mellitus is a growing problem in today's modern societies. It is difficult to estimate the total number of people suffering from the disease. Approximately 20% of diabetic patients develop wound infections during their life Which in the absence of effective tre MoreDiabetes mellitus is a growing problem in today's modern societies. It is difficult to estimate the total number of people suffering from the disease. Approximately 20% of diabetic patients develop wound infections during their life Which in the absence of effective treatment can disrupt the quality of life of these people. On the other hand, treatment of this complication is very costly. DFIs diabetic foot infections are one of the most important public health issues and the identification of microorganisms that cause microbial infections An antibiotic is good for finding an appropriate treatment. Meanwhile, many reports have shown that antibiotic resistance is rising dramatically. Therefore, early diagnosis of lesions and the rapid onset of antimicrobial treatment are essential for controlling infection and preventing complications and improving the quality of life. An antibiotic susceptibility test is needed to manage infection, which can help in choosing the best treatment options. Manuscript profile -
Open Access Article
2 - Active edible films and coatings with enhanced properties using nanoemulsion and nanocrystals
Fatemeh Kalateh Seifari Hamed Ahari -
Open Access Article
3 - Antimicrobial effect of Carvacrol on Aspergillus flavus and reduce expression of aflR gene in the aflatoxin biosynthetic pathway
Seyed Amir Ali Anvar Hamed Ahari Nasim Mojarrad -
Open Access Article
4 - Effect of chitosan coating contain Ajwain essential oil on the shelf-life of chicken breast meat during refrigerated condition
Nima Babolanimogadam Ali Khanjari Afshin Akhondzadeh basti Seyed Hasan Sajjadi Alhashem Reza Teimourifard Faramarz Amiri -
Open Access Article
5 - Evaluations of the antibacterial effect of chitosan edible film contain ajwain essential oil on some foodborne pathogenic bacteria
Nima Babolani Mogadam Najmeh Moghimi Peyman Mahasti Shotorbani -
Open Access Article
6 - Effect of bioactive edible coating based on sodium alginate and coriander (Coriandrum sativum L.) essential oil on the quality of refrigerated chicken fillet
Mina Kargozari Hassan Hamedi Seyyed Amir Amirnia Ahmad Montazeri Sara Abbaszadeh -
Open Access Article
7 - Study of antimicrobial effect of Artemisia sieberi in different grazing conditions on the Salmonella enteritidis
اشکان اطمینان اقطاعی بابک خیرخواه رضا باقریArtemisia sieberi is a plant from chicory category which is a green crucible and it is from thegrowing elements of Iran and Turani and it is from the prevailing elements of the Stopypasturage of Iran too. The application of this plant in the field that isn't for grasses MoreArtemisia sieberi is a plant from chicory category which is a green crucible and it is from thegrowing elements of Iran and Turani and it is from the prevailing elements of the Stopypasturage of Iran too. The application of this plant in the field that isn't for grasses is so muchvarious because of its expressed juice content.Antibiotic property of this plant is used in ancient medical to cure the abnormal digestion and toward of the intestinal parasites.The purpose of this study is considering of antimicrobial effect of the expressed juice of thisplant in different grazing conditions on the Salmonella enteritidis that create illness. Thisbacterium is one of the agent of intestinal infections. In this research the growing of Artemisiain current year from available sites have colleted in two different has been sites which in one ofthem the action of grazing done and in the other one the grazing action hasn't been done then theexpressed juice of this plant has been provided and its antimicrobial effect in 6 attendancesalong with its medicine properties which is provided in a diluted condition an the creatingillness bacteria of salmonella have been accomplished by using the spreading disk method inrelated to the assessing the antimicrobial property in different densities.The results of this research showed that the grazing factors had no effect on differentcombinations of antibacterial of expressed juice of Artemisia sieberi but antimicrobial effects ofthis plant relies on the grazing variable. The main environment of this plant is Muller HintonAgar and the results of this research have many applications in food and medicine industrials. Manuscript profile -
Open Access Article
8 - Study of the antioxidant and antimicrobial effects of the ethanolic extract of Eucalyptus camaldulensis Dehnh against infectious bacteria isolated from clinical and animal sources
Mahdavi, S., Haj Azimian, S., Isa Zadeh, A.R., Babash pour, M., Shishehgar, R. .Due to the increasing resistance of pathogenic bacteria to common antibiotics and antimicrobial agents, researchers are finding antimicrobial agents with plant origin as alternative drugs. Antioxidants are the main factors in neutralizing free radicals and prevent the s MoreDue to the increasing resistance of pathogenic bacteria to common antibiotics and antimicrobial agents, researchers are finding antimicrobial agents with plant origin as alternative drugs. Antioxidants are the main factors in neutralizing free radicals and prevent the spread of chronic diseases and destruction of many foods. After preparing the ethanolic extract from leaves of Eucalyptus camaldulensis Dehnh by maceration method, the antimicrobial effect of it was determined on Staphylococcus aureus and Escherichia coli isolated from women’s urogenital tract infections and food stuff and Salmonella pullorum and Salmonella gallinarum isolated from birds cases by minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) methods. The antioxidant and reducing power effects of ethanolic extract of Eucalyptus camaldulensis Dehnh were studied by DPPH(2,2-diphenyl-1-picryl-hydrazyl-hydrate) assay. The least to most antimicrobial effects of ethanolic extract of Eucalyptus camaldulensis Dehnh were seen against Salmonella pullorum, Escherichia coli, Staphylococcus aureus and Salmonellagallinarum, respectively. Antioxidant effect and reducing power of ethanolic extract of Eucalyptus camaldulensis Dehnh were recorded less than BHT (Butylatedhydroxytoluene) in equal concentration (p<0.05). Ethanolic extract of Eucalyptus camaldulensisDehnh leaves in high concentration has antimicrobial, antioxidant and reducing power, so it could be used to pharmaceutical industry for chemotherapy and disinfectants in control of human and animal diseases and as food preservatives. The aim of this investigation is study of the antioxidant and antimicrobial effects of the ethanolic extract of Eucalyptus camaldulensis Dehnh against infectious bacteria isolated from clinical and animal sources. Manuscript profile -
Open Access Article
9 - Survey Genotyping of Animal and Human Klebsiella pneumoniae Isolates using ERIC-PCR and evaluation of Antibiotic Sensitivity Pattern
Estabraghi, E., Zahraei Salehi, T.*, Amini, K., Jamshidian, M. .Klebsiella pneumoniae, is an opportunistic pathogens and cause infections in humans and animals. Drug resistant K. pneumoniae is rising. Therefore, antimicrobial susceptibility testing before prescribing antibiotics, it seems necessary. The aim of present study was to s MoreKlebsiella pneumoniae, is an opportunistic pathogens and cause infections in humans and animals. Drug resistant K. pneumoniae is rising. Therefore, antimicrobial susceptibility testing before prescribing antibiotics, it seems necessary. The aim of present study was to survey typing of clinical and animal K. pneumoniae isolates and evaluation of antibiotic susceptibility. A total 100 clinical and animal K. pneumoniae isolates were collected from Babak city. Antibiotic susceptibility was performed with Kirby-Bauer method according to CLSI guidelines. Then, DNA genomic extraction was done using DNA kit and PCR amplification was performed with ERIC1 and ERIC2 primer. Our results were shown that all strains (100%) were resistant to the ampicillin and amikacin antibiotics. The most and least resistance belong to tetracycline (53 strains; 88.3%) and imipenem (8 isolates; 13.3%), respectively. The results of cluster analysis and drawing dendrogram based on genetic similarities for 100 isolates was separated to seventeen distinct groups. According to our finding indicated an increasing resistance to antibiotics amongst K. pneumoniae. Additionally, the ERIC sequences have a pair of games that contain highly reversed and central reps and are located in the outermost regions of the bacterial genome and have less complexity in determining the genetic diversity of all isolates, but the separation good at the strain level. Manuscript profile -
Open Access Article
10 - Anti-bacterial effect of Madracis sp. and Palythoa tuberculosa soft coral extracts from Chabahar coasts
Taheri, A.*, Saki, N., Pouladi, S., Sharifi Torksalooye, E. .Nowadays due to the bacterial resistance to antibiotics, the need to new materials with antibacterial properties, including bioactive compounds from marine organisms such as corals with antibacterial properties, is more notable. So this study was conducted to evaluate t MoreNowadays due to the bacterial resistance to antibiotics, the need to new materials with antibacterial properties, including bioactive compounds from marine organisms such as corals with antibacterial properties, is more notable. So this study was conducted to evaluate the antibacterial effects of the methanolic, hydroethanolic and n-hexanic extracts of two soft marine coral Madracis sp. and Palythoa tuberculosa. After sampling, n-hexanic, methanolic and hydroethanolic extracts of two coral species of Madracis sp. and Palythoa tuberculosa was prepared. Then the disk diffusion method was used in order to determination the antibacterial activity against the three strains of Gram-negative bacterium include Vibrio cholera, Proteus vulgaris and Escherichia coli and two Gram-positive bacteria, Staphylococcus aureus and Listeria monocytogenes, and the results were compared with the standard antibiotic erythromycin. The analysis of the results was conducted by using one-way ANOVA whit SPSS software. The methanolic and hydroethanolic extracts of Madracis sp. corals had the highest effect on E. coli bacteria, with the inhibition zone of 12.94±9.0 and 16.58±0.53 mm, respectively. In all the extracts prepared from Palythoa tuberculosa Coral, standard antibiotic had more significant effect against the bacteria. The extracts of Madracis sp. coral has high antimicrobial properties. Manuscript profile -
Open Access Article
11 - Evaluation of survival and antioxidant properties of Zygosaccharomyces bisporus and Pichia occidentalis isolated from Kombucha
S. Eisazadehrazlighi, M. Khomeiri, S.M.H. Razavi, A. Moayedi, A. Ardebili محمد Gorbani E. Mahmoudi,Background and purpose: Some yeasts, such as Z. bisporis and P. occidentalis, still need further study to determine their functional characteristics and how to use them in the food and pharmaceutical industries. The purpose of this study is the investigation of the func MoreBackground and purpose: Some yeasts, such as Z. bisporis and P. occidentalis, still need further study to determine their functional characteristics and how to use them in the food and pharmaceutical industries. The purpose of this study is the investigation of the functional and antioxidant properties of Z. bisporis and P.occidentalis isolated from Kombucha. Materials and methods: for this purpose, antioxidant capacity by 1,1-diphenyl-2-picrylhydrazyl radical method (DPPH) and functional properties (survival under simulated conditions of the gastrointestinal system, hydrophobicity, auto aggregation and antioxidant properties, antimicrobial activity against food-borne pathogenic bacteria such as Staphylococcus aureus, Yersinia enterocolitica, Bacillus cereus and Escherichia coli was investigated by spot method. Results: The results of this research showed that Z.bisporis and P.occidentalis have excellent antioxidant activity of about 67.67 and 69.18 percent, auto aggregation capacity of about 92.44 and 87.30 percent. After 24 hours and high hydrophobicity activity has provided about 87.51 and 86.127 percent. Also, the survival percentage of these isolates under the simulated conditions of the gastric juice was about 54.87 and 88.87%, under the simulated conditions of the duodenum, it was 97.09 and 94.40%. Z. bisporis studied in this test had antimicrobial activity against Yersinia enterocolitica, but did not show any activity against the tested microorganisms. Conclusion: According to the obtained results, it is suggested to use more of these strains as a natural source of antioxidant and probiotic supplement in the production of food and pharmaceutical products after completing additional tests. Manuscript profile -
Open Access Article
12 - In vitro Effect of Carotenoid Pigment of Rhodotorula glutinis on Staphylococcus Aureus Isolated from Bovine Mastitis
S. Naisi, منصور Bayat, , T Zahraei Salehi, بهاره Rahimian Zarif, R. Yahyaraeyat,Mastitis, as a most costly disease of dairy cows, causes a lot of damage to the livestock industry in the world every year. One of the most important bacteria which causes clinical mastitis in cattle is S. aureus, which has become resistant to most common antibiotics in MoreMastitis, as a most costly disease of dairy cows, causes a lot of damage to the livestock industry in the world every year. One of the most important bacteria which causes clinical mastitis in cattle is S. aureus, which has become resistant to most common antibiotics in veterinary medicine. Therefore, researchers are looking for new methods in the treatment of such infections, in which the pigments of microorganisms fall into this category. The aim of this recent study was to optimize the production of carotenoid pigments by Rhododorula yeast and to investigate its antimicrobial effect on S. aureus isolates To isolate S. aureus, samples were taken from the milk of 100 cows with mastitis and the femA gene was identified for molecular confirmation among suspicious isolates of Staphylococcus by PCR method. Also, the isolate of R. glutinis obtained from one of the samples of mastitis was further examined and used as a sample of yeast producing pigment. The results showed that in addition to S. aureus as one of the causative agents of mastitis in dairy cattle, but also the yeast R. glutinis can be a causative agent of mastitis. The inhibitory effect on the growth of Staphylococcus using the pigment extracted from R. glutinis was also quite evident and more than 80% of S. aureus isolates were sensitive to the pigment at a concentration of 200 μg. R. glutenis inhibits excellent growth in S. aureus as one of the leading causes of mastitis in cows. The cause is the appearance of synthetic antibiotics and the reduction of microbial resistance. Manuscript profile -
Open Access Article
13 - Comparative evaluation of the antibacterial activity of Dill (Anethum graveolen L.) and garden Thyme (Thymus vulgaris) against of Salmonella Typhimurium (ATCC= 14028)
نازیلا Eslami, , Y , Anzabi, M.A Nour Azar,Salmonellosis is one of the most important common diseases of livestock and humans worldwide. With the prevalence of antibiotic resistance, the tendency to use medicinal plants to control microorganisms has increased. The aim of this research was to investigate the anti MoreSalmonellosis is one of the most important common diseases of livestock and humans worldwide. With the prevalence of antibiotic resistance, the tendency to use medicinal plants to control microorganisms has increased. The aim of this research was to investigate the antibacterial effects of the essential oils of Thymus vulgaris and salmonella typhimurium (ATCC=14028). For this purpose, first, the essential oils of the mentioned plants were extracted by steam distillation using a Clovenger machine. Then, using the microdilution broth method, the Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) of essential oils alone and in combination, as well as drug sensitivity or resistance testing based on the well diffusion method in agar against the standard strain of Salmonella typhimurium bacteria, were determined in comparison with three common antibiotics in veterinary medicine. The MIC was calculated for thyme (0.25), mixed essential oil (0.50), doxycycline (0.0078) and oxytetracycline (<0.00195) μg/ml. The MBC for them was 0.25, 0.50, 0.0156 and >0.00195 respectively. Dill essential oil and erythromycin antibiotic had no antimicrobial effect against Salmonella typhimurium. The highest effect was related to oxytetracycline (with a growth inhibition diameter of 27 mm) and the lowest effect was related to thyme essential oil (with a growth inhibition diameter of 10 mm). Unlike dill essential oil, garden thyme essential oil has a significant antibacterial effect. Manuscript profile -
Open Access Article
14 - Investigating the effect alcoholic extract of Nepeta crispa on the antioxidant activity and microbial and sensory properties of doogh
A. Haseli R. Pourahmad M. R. Eshaghi P. Rajaei B. Akbari-Adergani Introduction: Due to the richness of nutrients, especially at ambient temperature, doogh is prone to contamination with some microorganisms, which causes changes in the product flavor and its bloating during storage. Therefore, the use of natural antimicrobials su More Introduction: Due to the richness of nutrients, especially at ambient temperature, doogh is prone to contamination with some microorganisms, which causes changes in the product flavor and its bloating during storage. Therefore, the use of natural antimicrobials such as Nepeta crispa exract to increase doogh shelf life while increasing its marketability can be important. The aim of this research was to investigate the effect of Nepeta crispa alcoholic extract on the qualitative properties of doogh.Materials and Methods: The compounds of Nepeta crispa extract were identified using GC-MS. Antioxidant activity, physicochemical (pH, acidity and viscosity), microbial (Escherichia coli and Staphylococcus aureus count) and sensory (taste, odour, texture and overall acceptance) characteristics of doogh containing different concentrations of Nepeta crispa extract (1.25, 2.5 and 5 mg/ml) were investigated during storage. ANOVA and Duncan test were used for statistical analysis.Results: Thymol (53%) was the most identified compound in the extract of Nepeta crispa. The MIC of Nepeta crispa extract against E.coli and S.aureus was 2.5 and 1.5 mg/ml, respectively. The MBC of the extract against E.coli and S.aureus was 5 and 3 mg/ml, respectively. During storage, by adding plant extract, the acidity and viscosity of the samples increased and the pH decreased. Increasing the concentration of the extract increased the antioxidant and antimicrobial activity of doogh. The sample containing 2.5 mg/ml extract had the highest overall acceptance score, therefore, this sample was selected as the best treatment.Conclusion: Nepeta crispa extract has antimicrobial and antioxidant activities and might be used as a natural preservative in doogh. Manuscript profile -
Open Access Article
15 - Antioxidant and Antimicrobial Effect of Encapsulated Grape Extract in Nanochitosan-TPP on Shelf life of Surimi (Clupeonella cultriventris) in 4º ± 1 C
S. Soleymanfallah Zh. Khoshkhoo S.E. Hosseini M. H. AziziIntroduction: In this study, the effect of an aqueous extract of grape microencapsulated in chitosan nanoparticles (0.5 / 1) was used to evaluate the shelf life of surimi prepared from Kilka fish by ionic gelation method.Materials and Methods: Grape was extracted aqueou MoreIntroduction: In this study, the effect of an aqueous extract of grape microencapsulated in chitosan nanoparticles (0.5 / 1) was used to evaluate the shelf life of surimi prepared from Kilka fish by ionic gelation method.Materials and Methods: Grape was extracted aqueously, and total phenol content was detected by spectrophotometry and phenolic acids of the extract by HPLC method. Physical properties of nanoparticles including particle size, zeta potentia and PDI were determined with zeta-sizer, and microencapsulation efficiency was determined by spectrophotometer and loading capacity of nanoparticles by HPLC method. Antioxidant activity of free extract and chitosan nanoparticles with / without extract (by DPPH test) was determined. The total count was determined by cryophilic, mesophilic, pseudomonas, mold, and yeast and inhibition capacity (MIC, MBC and IZ) of selected Gram positive and Gram negative bacteria and mold and yeast in different treatments days (0 , 1 , 3 , 6 and 9 ) at refrigeration temperature.Results: The data showed that the produced nanoparticles were in a favorable condition (particle size, zeta potential and PDI: 177.5 nm, +32.95 mV and 0.385 and the efficiency of microencapsulation and loading of nanoparticles were 48.95 and 6.19 percent, respectively). The phenolic content of the extract was 2896 ±18 mg/g and the extract in the loaded state showed a higher percentage of antioxidant activity (42.1%). Growth inhibition in microorganisms (gram positive and negative) is also increased and the overall amounts of bacteria and mold and yeast decreased, and the total values of cryophilic bacteria (6.35 ± 0.29 CFU/g), mesophilic bacteria (6.18 ± 0.27 CFU/g), Pseudomonas (5.41 ± 0.14 CFU/g), and mold and yeast (2.45 ± 0.26 CFU/ /g) in the last day of experiment, showed a significant decrease in the growth of microorganisms. Conclusion: According to the results, the use of chitosan nanoparticles containing extract in surimi of Kilka fish can delay microbial decay and increase the product resistance to free radicals and thus increase the shelf life of the product during the storage period in the refrigerator. Manuscript profile -
Open Access Article
16 - The effect of Suaeda aegyptiaca extract on yogurt initiator and its antioxidant and organoleptic properties
Sahar Zolghadr Shabnam Haghighat KhajaviIntroduction: Due to the changes in people's tastes, the producers try to produce newproducts with high nutritional value. Today, using vegetables for flavoring yogurt has becomevery popular among consumers.Materials and Methods: In this study, prepared Suaeda aegyptiac MoreIntroduction: Due to the changes in people's tastes, the producers try to produce newproducts with high nutritional value. Today, using vegetables for flavoring yogurt has becomevery popular among consumers.Materials and Methods: In this study, prepared Suaeda aegyptiaca extract was used at theconcentrations of 0.05, 0.1, 2, and 2.5% (w / w) in the formulation of low-fat yogurt. Theantioxidant and antimicrobial properties of the extracts were evaluated, followed byinvestigating the that physical-chemical, microbial, and organoleptic properties of thetreatment during 28 days at 4 ° C.Results: The result showed that increasing the concentration of the extract was notaccompanied by a significant change in the account of starter cultures and also the content ofstarter culture increased during storage. Moreover, Staphylococcus aureus was significantlyinhibited while Ecoli and Aspergillus niger were inhibited after 14 and 21 days of storage.Furthermore, by increasing the amount of extract, DPPH assay and phenolic compounds werecompound significantly increased and the storage time significantly affected the free radicalscavenging and TPC capacity of the yogurt. During storage, pH decreased while acidity andsyneresis increased and water holding capacity, and viscosity decreased. Color measurementshowed a significant difference in color between different kinds of yogurts. Supplementationwith plant extracts, L*and a* values decreased while b*values increased.Conclusion: In the case of sensory characteristics of yogurt, samples that have 0.05,0.1 % ofextracts were not significantly different in overall acceptability from plain yogurt. Manuscript profile -
Open Access Article
17 - The Antimicrobial Effect of Chitosan Nano Particles Film on Two Species of Food Bacteria
P. Rezaee روحا Kasra KermanshahiIntroduction: Unbiodegradable materials used in food packaging and the microbial contaminants derived from this might create problems in our society. The application of biodegradable material that might have antimicrobial activity is desirable and is considered a priori MoreIntroduction: Unbiodegradable materials used in food packaging and the microbial contaminants derived from this might create problems in our society. The application of biodegradable material that might have antimicrobial activity is desirable and is considered a priority for the researchers. Chitosan is a biopolymer that eliminates these problems. Materials and Methods: In this study, chitosan nanoparticles and chitosan films were prepared and physical and chemical characteristics of these were determined and the antimicrobial effects against Staphylococcus aureus PTCC 1431 and Listeria monocytogenesusing different methods were assessed. Results: The size of chitosan nanoparticle was 273 nm with MIC and MBC of 3.5 for these bacteria. Synthezied chitosan film with 43.69 ± 2.4 mm of thickness, 1% of moisture content and 52% film solubility caused a reduction of 100% in the number of food pathogenic bacteria in 24 hoursat 30°C. Conclusion: Since chitosan is a biodegradable polymer and has antimicrobial activity in the form of chitosan nanoparticle and film against food pathogenic bacteria (Staphylococcus aureus PTCC 1431 and Listeria monocytogenes), it might be employed in the packaging industry particularly food. Manuscript profile -
Open Access Article
18 - Studying the Effects of pH and Storage Time on Antioxidant and Antimicrobial Activities of Essential Oil and Extracts of Lemon Verbena (Lippia citriodora) in Model Food System
A. A. Gholamhosseinpour S. M. B. Hashemi S. ShokriIntroduction: Lippia Citriodoraplantis is a rich source of flavonoids that can reduce the microbial and oxidative deteriorations of food in general and therefore can extend the shelf-life of the food systems. Materials and Methods: For evaluation of the antimicrobial a MoreIntroduction: Lippia Citriodoraplantis is a rich source of flavonoids that can reduce the microbial and oxidative deteriorations of food in general and therefore can extend the shelf-life of the food systems. Materials and Methods: For evaluation of the antimicrobial activity of essential oil and extract, the treated food emulsions by 1% and 2% essential oil and aqueous-alcoholic extract at the pH of 5, 7 and 9 were employed. Peroxide value, anisidine value, conjugated dienes, as oxidative parameters, and also total counts of microflora were measured during eight days of storage. Results: Essential oil and aqueous-alcoholic extract showed good antimicrobial activities under the experimental conditions. There were not significant differences (p ≤ 0.05) in the results concerned with DPPH and FRAP between essential oil and aqueous-alcoholic extract. The results of antimicrobial and antioxidant activities of essential oil and aqueous-alcoholic extract in model food system showed that both essential oil and extract decreased the total counts of microorganisms and the amounts of oxidative rancidity as compared to the control during 8 days of storage. Antimicrobial and antioxidant activities of essential oil and extract showed a direct correlation with the pH, therefore the mentioned activities were higher at lower pH values. Conclusion: The results of this study showed that the essential oil and extract of Lippia Citriodora can be used as antioxidant and antimicrobial agents in food products. Manuscript profile -
Open Access Article
19 - Chemical Modification of Lysozyme with Dextran by Using Maillard Reaction and Evaluating the Antimicrobial Properties of the Modified Enzyme
Mahmood Aminlari Roghayeh Ramezani sedigheh amiriIntroduction: Lysozyme is a natural enzyme with positive antimicrobial activity against Gram Positive bacteria, but its action is limited on Gram Negative bacteria, which is assumed a restriction for its uses in industry. The purpose of this research was to glycos MoreIntroduction: Lysozyme is a natural enzyme with positive antimicrobial activity against Gram Positive bacteria, but its action is limited on Gram Negative bacteria, which is assumed a restriction for its uses in industry. The purpose of this research was to glycosylate lysozyme with dextran through Maillard-based reaction and to study the antimicrobial characteristics of lysozyme-dextran conjugate.Materials and Methods: Glycosylation of lysozyme with dextran was performed using a 1:5 weight ratio of protein to dextran, incubated at 60 MC for one week under the relative humidity of 79%. For evaluating conjugation of dextran to lysozyme, SDS-PAGE electrophoresis was conducted. Gel filtration chromatography with sephadox G-100 was used for separation of conjugated enzyme. Lytic activity, free amino group and antimicrobial activity of the modified enzyme were evaluated.Results: SDS-PAGE electrophoresis was used to follow the glycosylation process. Results indicated that 3.7 moles of dextran were coupled to one mole of lysozyme. The lytic activity of the conjugate was about 62% of that of the native lysozyme. Evaluation of antimicrobial activity of the lysozymedextran conjugate, indicated the effectiveness of modified enzyme against E. coli and a progressive increase in antimicrobial activity with an increase in enzyme-conjugate concentration. The antimicrobial action of lysozyme on S. aureus was not improved by conjugation with dextran as compared with that of E. coli.Conclusion: These results might increase the application of lysozyme as a natural antimicrobial ingredient in different food systems. Manuscript profile -
Open Access Article
20 - The Antimicrobial and Organoleptic Effects of Mentha aquatica Essential Oil on Kluyveromyces marxianus in Iranian Yoghurt Drink
S. Aghdasi H. Kaboosi L. GolestanIntroduction: Mentha aquatica is one of the species of mint family. The essential oil of this plant has shown effective antimicrobial properties on various kinds of microorganisms in the culture. This study aims to investigate the preventive influence of Mentha aquatic MoreIntroduction: Mentha aquatica is one of the species of mint family. The essential oil of this plant has shown effective antimicrobial properties on various kinds of microorganisms in the culture. This study aims to investigate the preventive influence of Mentha aquatic essential oil on viability of Kluyveromyces marxianus in Iranian yoghurt drink. Materials and Methods: Mentha aquatic essential oil was obtained by distillation method employing Clevenger apparatus. The effective composition of the essential oil was identified by Gas Chromatography equipped with Mass Spectrometer (GC/MS). The inhibitory effect of Mentha aquatic essential oil on kluyveromyces marxianus was examined on Muller Hinton agar culture using diffusion disk method. After assurance of its antimicrobial properties, the Inhibitory influence of Mentha aquatic essential oil was studied on Kluyveromyces marxianus at 0.05, 0.1, 0.125 and 0.25% concentrations in Iranian drinking yoghurt during 28 days of storage. Results: The results showed that Mentha aquatica essential oil has the highest inhibitory effect on Kluyveromyces marxianus growth at high concentrations. Concerning the antimicrobial property and organoleptic characteristics, it might be concluded that 0.1% concentration of the essential oil might be regarded as the optimum concentration. Conclusion: It can be concluded that Mentha aquatic essential oil, caused a declining effect on the viability of microorganism studied. Manuscript profile -
Open Access Article
21 - The Effect of ZnO Nanoparticles on the Growth of Bacillus subtilis and Escherichia coli O157:H7
hakimeh esmaielzadeh parvaneh sangpour ramin khaksar farzaneh shahrazIntroduction: Recently antimicrobial nanoparticles such as ZnO have attracted the attentions of scientists. Regarding the antimicrobial effect and low cost of ZnO nanoparticles, these might be emplyed in food industry to reduce the growth of food microorganisms. The obj MoreIntroduction: Recently antimicrobial nanoparticles such as ZnO have attracted the attentions of scientists. Regarding the antimicrobial effect and low cost of ZnO nanoparticles, these might be emplyed in food industry to reduce the growth of food microorganisms. The object of this study is to evaluate the antibacterial effect of ZnO nanoparticles on Bacillus Subtilis and E.Coli O157:H7, two important spoiling and pathogenic bacteria of food.Materials and Methods: ZnO nanoparticles have been synthesized by molten salt method and have been characterized by X-ray diffraction, and scanning electron microscopy. Bacillus Subtilis and E.Coli O157:H7 have been inoculated in 2% and 4% w/w ZnO containing tryptic soy broth followed by the plot of their growth curve.Results: Treatments with both 2% and 4% concentrations of ZnO nanoparticles significantly reduced the number of E.Coli O157:H7 (p=0.001 and p Conclusion: Regarding the results of the present study, ZnO nanoparticles can significantly reduce the number of both bacteria however it seems that the effect for gram negative bacteria might be more dramatic. Manuscript profile -
Open Access Article
22 - The Study of Antimicrobial Effect of Silver Nanoparticles Biosynthesized by the Leaf Aqueous Extract of Kelussia odoratissima Mozaff. against Some Pathogenic Microbes with Food Sources
O. Azizian Shermeh M. Taherizadeh M. Valizadeh A. Ghasemi M. Beigomi A. Kamali DeljooIntroduction: Growth of food pathogenic microorganism is one the major obstacles in the foodindustry. Nano technology has a broad application in all parts of food industry. The developmentof bacterial resistance to antibiotics and antimicrobial effect of silver nanopart MoreIntroduction: Growth of food pathogenic microorganism is one the major obstacles in the foodindustry. Nano technology has a broad application in all parts of food industry. The developmentof bacterial resistance to antibiotics and antimicrobial effect of silver nanoparticles, has caused theattention of researches to this subject. In this study, biosynthesis of silver nanoparticles and theeffective parameters concerned and the antimicrobial effect of them against some food pathogensfor use in antimicrobial packaging has been discussed.Materials and Methods: Silver nanoparticles were synthesized by the leaf aqueous extract ofKelussia odoratissima Mozaff. In order to achieve Silver nanoparticles with a uniform shape andminimum size, effective parameters on synthesis such as: pH of reaction, extract volume,concentration of Ag+ and reaction time, were studied and optimized by UV-Visspectrophotometry. After characterization of nanoparticles using TEM and XRD techniques, theirantimicrobial activities were investigated against Staphylococcus aureus, Bacillus cereus,Escherichia coli, Salmonella tifimurium,Listeria monocytogenes 4b, Aspergilus Flavus,Penicillium expansum, Clavicpes purpurea by Agar Well Diffusion and MIC, methods.Results: Silver nanoparticles that had been biosynthesized with the approximate size of 20-25nm,had high antimicrobial activity against all of microorganisms and this activity was dependent onthe concentration of silver nanoparticles, therefore in very low concentrations, they prevented thegrowth of microorganisms.Conclusion: Biosynthesis of silver nanoparticles, made nanoparticles with minimum size andbetter performance. Currently these nanoparticles can be use in food industry as food disinfectantfilters, food coatings and packaging and cleaning the food pruduction lines. Manuscript profile -
Open Access Article
23 - Antimicrobial Effects of Cold Plasma on the Pathogenic Bacterium Salmonella enteritidis Existed on the Egg Shell
P. Bohlouli R. Jalalirad D. DorranianIntroduction: Cold plasma or non-thermal plasma technology is one of the methods of foodprocessing that is used to inactivate pathogen microorganisms and improve food safety. Coldplasma can affect the inactivation of a wide range of microorganisms, without harming theho MoreIntroduction: Cold plasma or non-thermal plasma technology is one of the methods of foodprocessing that is used to inactivate pathogen microorganisms and improve food safety. Coldplasma can affect the inactivation of a wide range of microorganisms, without harming thehost and healthy tissues.The aim of this study was to evaluate the effect of cold plasma directly on reducing thenumbers of Salmonella enteritidis on egg shell and also to determine the effect of plasmaexposure time and the composition of the injected gas.Materials and Methods: In this study, the effect of cold atmospheric plasma radiation ofargon and argon containing 5% oxygen gas, at the rate of 3 liters / min, and at three differenttimes on inactivation of Salmonella enteritidis was investigated.Results: The effect of argon gas radiation for 1, 3 and 6 minutes on the reduction ofsalmonella enteritidis numbers being 4.490, 3.948, 0 cfu /ml respectively, was significant at1% probability level. Also, the radiation effect of argon containing 5% oxygen at 1, 3 and 6minutes,on reduction of Salmonella enteritidis numbers was 4.559, 4.226 and 0 cfu /ml,respectively. Both groups of the treatments caused a significant decreasing trend at thestatistical level of 1% as compared to the control sample.Conclusion: The obtained data indicated that the effects of the gas type, as well as theirradiation time and their interaction on the tested bacterium were statistically significant atp>0/01 as compared to the control. Manuscript profile -
Open Access Article
24 - Antimicrobial Effects of Grape and Pomegranate Waste Extracts against two Foodborne Pathogens
M. Javanmard -
Open Access Article
25 - The Use of Chitosan and Whey Protein Isolate Edible Films Incorporated with Zataria multiflora Boiss. Essential Oil as an Active Packaging Ingredient Against Some Common Foodborne Bacteri
S. Shoja Gharehbagh A. Khanjari M. Yeganmohammadi Davaji A. Akhondzadeh Basti -
Open Access Article
26 - Antimicrobial Peptides Derived from Milk: A Review
E. Kamali Alamdari M. R. Ehsani -
Open Access Article
27 - Antibacterial Effects of Stevia rebaudiana Bertoni Extract on Pathogenic Bacteria and Food Spoilage
M. Fallah Shojaee A. R. Sadeghi Mahoonak M. Khomeiri M. Ghorbani -
Open Access Article
28 - Eco-Friendly Synthesis of Magnetic Iron Oxide Nanoparticles Using Achillea Nobilis Extract and Evaluation of Their Antioxidant and Antibacterial properties
S. Mohamad Ebrahimzadeh Sepasgozar Sh. Mohseni B. Feyzizadeh A. Morsali -
Open Access Article
29 - Antimicrobial Peptides Derived from Goat’s Milk Whey Proteins Obtained by Enzymatic Hydrolysis
M. Esmaeilpour M. R. Ehsani M. Aminlari Sh. Shekarforoush E. Hoseini -
Open Access Article
30 - Synthesis and Characterization of Silver Nanocomposite as a Food Packaging
M. Pooyamanesh H. Ahari A. A. Anvar G. Karim -
Open Access Article
31 - Study of Antimicrobial Properties of Nano Chitosan with Marjoram Essential Oil in Increasing the Shelf-Life of Shrimp (Nephropidae) at Refrigerated Temperature
Z. Saeidi H. Ahari S.A.A. Anvar -
Open Access Article
32 - Evaluation of antibacterial activity of magnesium oxide nanoparticles in ZSM-12 zeolite obtained from rice husk
Azita Albouyeh afshin pourahmad Hassan KefayatiBackground and Objective: Rice husk is an agricultural by-product material comprising about 22% of the weight of rice. Even though some of this husk is converted into end products such as feedstock and adsorbent most is burnt openly, causing environmental and health pro MoreBackground and Objective: Rice husk is an agricultural by-product material comprising about 22% of the weight of rice. Even though some of this husk is converted into end products such as feedstock and adsorbent most is burnt openly, causing environmental and health problems especially in poor and developing countries. Therefore, it is very important to find pathways to fully utilize the rice husk. Material and Methodology: In this study, the fabrication and characterization of ZSM-12 zeolite using rice husk ash as an alternative cheap silica source, and synthesis and characterization of magnesium oxide nanoparticles in ZSM-12 matrix by solid state reaction and ion exchange methods is reported. Disk diffusion method and MIC were used to evaluate the antimicrobial activity of nanoparticles on gram-negative (Escherichia coli) and gram-positive (Staphylococcus aureus) strains of bacteria compared to standard commercial antibiotic disks. Findings: Synthesized samples were evaluated using X-ray diffraction, scanning electron microscopy, energy dispersive X-Ray spectroscopy and transmission electron microscopy. XRD results revealed diffraction peaks for each of the two compounds in the MgO/ZSM-12 nanocomposite. Discussion and Conclusion: Transmission electron microscopy image of nanocomposite indicated that nanoparticles size of MgO is ~ 35 nm. Nanocomposite exhibited antibacterial activity with minimal inhibitory concentration of 96 µg/mL against E. coli and S. aureus. Manuscript profile -
Open Access Article
33 - Comparison of antimicrobioal effect of essential oil of Vitex agnus – castus with common antibiotics in vitro.
mohammad dakhili Hamideh Afarin محمدرضا ذوالفقاریVitex agnus – castus (VAC) as a plant in herbal medicine has been since centuries ago. It is a phytoestrogenic herb native to the Middle East and southern Europe. It has clinical usage in so many countries. In this research, the antimicrobial effect of essential o MoreVitex agnus – castus (VAC) as a plant in herbal medicine has been since centuries ago. It is a phytoestrogenic herb native to the Middle East and southern Europe. It has clinical usage in so many countries. In this research, the antimicrobial effect of essential oil of VAC was investigated on Staphyloccocus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and was compared with Ampicilin, Gentamicin, Nystatin.Essential Oil of VAC was extracted by steam distillation, Chemical constituents was analyzed by gas chromatography-mass spectrometry. for evaluation of antimicrobial effect Disk diffusion and Macrodilution methods were used.Essentical oil of VAC inhibited the growth of Staphylococcus aureus and Candid albicans at Concentrations 56/25 and 112/5 respectively. by Disk diffusion method The inhibitory zones were 10 mm and 11 mm respectively This essential oil inhibited the growth of Pseudomonas aeruginosa and Escherichia coli at concentration 450 . by Disk diffusion method They didn’t show inhibitory zone.The results of this research indicated that the essential oil of VAC has antimicrobial effect, in comparison with antibacterial effect of Ampicilin and Gentamicin has significant difference (P<0/001) antifungal effect of this essential oil in comparsion with Nystatin doesn’t have significant difference (P>0/05). The majer constituents of oil were: Terpinolene, Caryophyllene, α-pinene. Manuscript profile -
Open Access Article
34 - Green Synthesis of zinc oxide Nanoparticles Using Apple Fruit and investigation its Antimicrobial Effects
Fateme Sadat Hoseini زهرا آقاجانیIn this research, zinc oxide nanoparticles (ZnO) were synthesized using apple extract, and then the physical and chemical properties of ZnO nanoparticles were evaluated using SEM, XRD, EDS, UV-Vis and FT-IR. The average size of ZnO nanoparticles using Debye-Scherrer equ MoreIn this research, zinc oxide nanoparticles (ZnO) were synthesized using apple extract, and then the physical and chemical properties of ZnO nanoparticles were evaluated using SEM, XRD, EDS, UV-Vis and FT-IR. The average size of ZnO nanoparticles using Debye-Scherrer equation was estimated at 10 nm. The nanoparticles synthesized by this method (using apple extract) exhibited antimicrobial properties against Candida albicans, which showed the diameter of the halo created by the specimen in this fungus was 26 mm. The XRD spectrum represents the formation of zinc oxide nanoparticles and is consistent with earlier reports. According to the SEM spectrum, zinc oxide is composed of uniform particles. According to the results of the EDS test, the presence of Zn in the synthesized nanoparticles has been confirmed using apple extract. In this research, zinc oxide nanoparticles (ZnO) were synthesized using apple extract, the physical and chemical properties of ZnO nanoparticles were evaluated using SEM, XRD, EDS, UV-Vis and FT-IR evaluation methods. The average size of nanoparticles ZnO was estimated using a 10-nm DSS equation. The synthesized particles with this method (using apple extract) showed antimicrobial properties against Candida albicans, which showed that the diameter of the halo created by the sample The mushroom was spotted at 26 mm. Manuscript profile -
Open Access Article
35 - Synthesis of zinc oxide nanoparticles with antimicrobial and antibiofilm activity against Klebsiella pneumonia
razieh ahmadinasab soheil Aghaei Mohammad Ali GasemzadehKlebsiella peneumoniae bacterium is part of the natural microfrola of the human body and are found in water, sewage, soil and part of the mouth, pharynx and intestines. Klebsiella can also cause infections in the urinary tract, lower biliary tract, and surgical wound si MoreKlebsiella peneumoniae bacterium is part of the natural microfrola of the human body and are found in water, sewage, soil and part of the mouth, pharynx and intestines. Klebsiella can also cause infections in the urinary tract, lower biliary tract, and surgical wound sites. It is also an opportunistic pathogen for patients with chronic pulmonary disease, enteric pathogenicity, nasal mucosa atrophy, and rhinoscleroma. This organism can causes food poisoning and gastritis. Biofilm formation by this bacterium on many surfaces, is one of the most important problems in the food industry. Metal nanoparticles are effective antimicrobial compounds for controlling and removing of bacterial biofilms from non-biomaterial surfaces. The main purpose of this investigation was synthesis of zinc oxide nanoparticles in-vitro condition and evaluation of their antimicrobial properties for inhibiting of biofilm formation and biofilm eradication of Klebsiella peneumoniae (ATCC700603). Method: In this study nano-particles of zinc oxide were synthesized mechanochemically and were confirmed using UV-vis, FTIR, EDX and Scanning Electron Microscopic procedures. Antimicrobial and antibiofilm properties of synthesized nanoparticles were determined using agar well diffusion and 96 well microplate dilution methods respectively. Results: Synthesized zinc oxide nanoparticles had circular structure with 30 nm size. Biofilm formation of kiebsiella pneumonia was performed using micrtiter plate assay. Antibiofilm activity and biofilm eradication of zinc oxide nanoparticles were obtained at 50 and 500 µg/ml respectively. Conclusions: Our study showed that zinc oxide nanoparticles can be used as the effective antimicrobial agents for inhibiting of biofilm formation of Klebsiella peneumoniae in the food industry. Manuscript profile -
Open Access Article
36 - study the effect of silver nanoparticles against to biofilm in listeria monocytogenes
razieh ahmadinasab soheil Aghaei Mohammad Ali GasemzadehListeria monocytogenes has been isolated from many foods and may cause meningitis, septicemia and abortion in pregnant women. L. monocytogenes forms biofilm on many food contact surface materials and medical devices. Biofilm formation by this bacterium on many surfaces, MoreListeria monocytogenes has been isolated from many foods and may cause meningitis, septicemia and abortion in pregnant women. L. monocytogenes forms biofilm on many food contact surface materials and medical devices. Biofilm formation by this bacterium on many surfaces, is one of the most important problems in the food industry. Metal nanoparticles are effective antimicrobial compounds for controlling and removing of bacterial biofilms from non-biomaterial surfaces. The main purpose of this investigation was synthesis of silver iodide nanoparticles in-vitro condition and evaluation of their antimicrobial properties for inhibition of biofilm formation and biofilm eradication of Listeria monocytogenes(ATCC1298). Method: In this study nano-particles of silver iodide were synthesized respectively sonochemically and were confirmed using UV-Vis ,FTIR, EDX and Scanning Electron Microscopic procedures. Antimicrobial and antibiofilm properties of synthesized nanoparticles were determined using agar well diffusion and 96 well microplate dilution methods respectively. Results: Synthesized Silver nanoparticles had circular structure with 30 nm size. Antibiofilm activity and biofilm eradication of Iodide silver nanoparticles were obtained at 5 and 100 µg/ml respectively. Discussion: Based on obtained results, silver iodide nanoparticles can be used as the effective antimicrobial agents for inhibiting of biofilm formation of Listeria monocytogenes in the food industry. Manuscript profile -
Open Access Article
37 - Study of antioxidan and antimicrobial effects of aqueous and alcoholic extract of plant ( Lawsonia inermis, punica granatum, walnut, Myrtus) on gram positive and gram negative bacteria
simin khosravi zahra rezayatmand mozhgan ghiasianRecently , due to the resistance of bacterial pathogens to the detection of new antimicrobials, much attention has been paid. In the present study, the antimicrobial properties of four extracts of leafy leafy leaves, henna leaves, walnut shells and pomegranate peel on t MoreRecently , due to the resistance of bacterial pathogens to the detection of new antimicrobials, much attention has been paid. In the present study, the antimicrobial properties of four extracts of leafy leafy leaves, henna leaves, walnut shells and pomegranate peel on three gram-positive bacteria, Staphylococcus aureus, Staphylococcus epidermidis and Bacillus subtilis, and two gram-negative bacteria of Escherichia coli and Pseudomonas aeruginosa were studied. it placed. Antimicrobial effect of the alcoholic and aqueous extract of these plants was studied by diffusion and well discarding method by measuring the non-growth aura's diameter and the minimum inhibitory concentration was investigated by ELISA method. The results show that in two methods, diffusion discs and wells in alcoholic and aqueous extracts have the highest effect on Staphylococcus epidermidis bacterium and have the least effect on E. coli bacteria. The minimum amount of MIC for alcoholic extract is 31.25 and MBC 54.25 mg / ml, which is related to Escherichia coli bacteria. The results measurements of total phenolic compounds, flavonoids and tannins also indicate that the highest amount of this product is in the concentration of 500 mg / ml of the extract of plants. Also, the amount of antioxidant activity in the alcoholic extract is about twice as much as the aqueous extract. The results of this study indicate that the combination of extracts of plants, especially alcoholic extract, has a more antibacterial effect than the aqueous extract, which can be due to the presence of more antibacterial compounds in the extract. Manuscript profile -
Open Access Article
38 - Synthesis, In vitro Antimicrobial and Cytotoxic Activities of Some Novel Bis- 1, 3, 4-oxadiazoles
Pavana Teja Pudota Raghunandan Shurpali Madhusudan Purohit G.V. Pujar -
Open Access Article
39 - Preparation of Antimicrobial Hydrogel Film Based on Chitosan, Poly Vinyl Poly Pyrrolidone, and Copper Ions
Ensieh Ghasemian Lemraski Elaheh khajehali Maryam Dashti Shirin Alibeigi -
Open Access Article
40 - One-pot Synthesis of 2-amino-4H-chromene Derivatives as Potential Antimicrobial Agents using DABCO-CuCl Complex as an Effective Catalyst
Bita Baghernejad Samaneh Koosha -
Open Access Article
41 - A study of the composition and antimicrobial activities of stored and freshly extracted leaf essential oil of Ocimum gratissimum L.
Ezekiel Olugbenga Akinkunmi Gbadegesin Adetogun Ibitola Odusegun -
Open Access Article
42 - Chemical constituents from the leaves and liana of Salacia nitida (Benth.) N.E.Br. (Celastraceae) and their antimicrobial activities
Brice Mba’ning Joël E. T. Ateba Angelbert F. Awantu Luciana S. Amaral Gervais M. Happi Beate Neumann Georg Stammler Bruno Lenta Silvère A. Ngouela Iran Malavazi Etienne Tsamo Norbert Sewald Edson Rodrigues-Filho -
Open Access Article
43 - Antimicrobial efficacy and chemical constituents of pseudo-stem essential oils from Zingiber castaneum
Le Thi Huong Trinh Thi Huong Nguyen Thi Bich Nguyen Thi Viet Abdulatif Olufemi Giwa-Ajeniya Isiaka Ogunwande -
Open Access Article
44 - Phytochemical analysis and in vitro antimicrobial screenings of the methanolic stem bark extract and constituents of Parkia bicolor A. Chev. (Leguminosae)
Aicha De Nkainsa Simplice Chimi Fotso Angelbert Awantu Fusi Francioli Korokoro Toze Flavien Aristide Alfred Jean Duplex Wansi Paul Désiré Dzeufiet Djomeni Alain Bertrand Dongmo Théophile Dimo -
Open Access Article
45 - Chemical constituents, antimicrobial and antioxidant activities of Leptoderris brachyptera (Benth.) Dunn and Leptoderris micrantha Dunn essential oils
Ganiyat Oloyede Michael Ibok Thomas Ojo -
Open Access Article
46 - Chemical constituents and antimicrobial activities of some isolated compounds from the Cameroonian species of Senna alata (Cassia alata L. Roxb synonym, The plant list 2013). (Leguminosae)
Simplice Chimi Fotso Alain Tcho Tadjong Willifred Dongmo Tekapi Tsopgni Bruno Lenta Celine Nkenfou Jean Duplex Wansi Flavien Aristide A. Toze -
Open Access Article
47 - A review on biosynthesis, regulation, and applications of terpenes and terpenoids
Anjali Singh Dhananjay Singh Sapna Sharma Nishu MittalEssential oils (EOs) are concentrated liquids extracted from various parts of plants and can be classified based on their phytochemical compounds. Terpenes and terpenoids have a wide range of biological activities, including anticancer, anti-inflammatory, antimicrobial, MoreEssential oils (EOs) are concentrated liquids extracted from various parts of plants and can be classified based on their phytochemical compounds. Terpenes and terpenoids have a wide range of biological activities, including anticancer, anti-inflammatory, antimicrobial, antioxidant, and antiallergic properties. Terpenes are plant-based compounds commonly used in the pharmaceutical, food, biofuel, and chemical industries by humans. In synthetic biology, genomic resources and emerging tools facilitate the production of high-quality terpenoids in plants and microbes. Terpenoids, however, are difficult to produce in large quantities due to their complex chemical structures and the limited amounts found in plants. The regulation of terpenoid biosynthesis has gradually emerged as a research priority. This review presents an overview of the biological activities, synthesis pathways, and key enzymes involved in the biosynthetic pathways and regulation of terpenes or terpenoids. This review will also include references for further research on molecular regulation, biological advancements, and increasing the content of terpenes or terpenoids in plants. Manuscript profile -
Open Access Article
48 - Chemical constituents, antioxidant and antibacterial activities of the hexane extract of Alchemilla sericata Reichenb
Ali Shafaghat Akram Panahi Masoud Shafaghatlonbar -
Open Access Article
49 - Phytochemicals and phyto-extracts in cosmetics
Satyajit D. Sarker Lutfun Nahar -
Open Access Article
50 - Antimicrobial activity and toxicity of Eucalyptus globulus Labill. essential oil against vaginal microorganisms
Mirjana Bogavac Kristina Tešanović Jovana Marić Mirjana Jovanović Maja Karaman -
Open Access Article
51 - Sonneratinone: A new antimicrobial benzofuranone derivative from the endophytic fungus Aspergillus niger isolated from the mangrove plant Sonneratia apetala Buch.-Ham
Tauhidur Rahman Nurunnabi Shaymaa Al-Majmaie Lutfun Nahar Ismini Nakouti S. M. Mahbubur rahman MD. Hossain Sohrab MD. Morsaline Billah Fyaz MD. Ismail George P. Sharples Satyajit D. Sarker -
Open Access Article
52 - Erratum: Nano-encapsulation of thyme essential oil in chitosan-Arabic gum system: Evaluation of its antioxidant and antimicrobial properties
Maryam Hasani Shirin Hasani -
Open Access Article
53 - Antioxidant, antimicrobial and anti-prostate cancer activity of the extracts from different parts of Etlingera velutina (Ridl.) R. M. Sm (Zingiberaceae)
Behnam Mahdavi Majid Mohammadhosseini -
Open Access Article
54 - Evaluation of the Activity of New Species of Jelly Fish Collected from Nayband Bay in Bushehr Against Human Pathogenic Bacteria
Akram Najafi Zahra Amini Khoei Saeid Tajbakhsh Golandam Asayesh Gholam hossein MohebbiInroduction & Objective:Currently, the presence of a wide range of bioactive compounds have been reported from the marine organisms worldwide. This study, for the first time, was aimed to evaluate the antimicrobial activity of water extraction of new species of jelly fi MoreInroduction & Objective:Currently, the presence of a wide range of bioactive compounds have been reported from the marine organisms worldwide. This study, for the first time, was aimed to evaluate the antimicrobial activity of water extraction of new species of jelly fish collected from Nayband Bay in Bushehr province. Material and Methods:In this study the jelly fish samples (Cassiopea andromeda) were collected from the Nayband Bay in Bushehr province. Minimum Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) of the jelly fish extract were determined against five human pathogens. All the data were analyzed by SPSS software.Results: Our results showed that the jelly fish extract has both MIC and MBC effects against Escherichia coli and Pseudomonas aeruginosa bacteriaThis relation was statistically significant.Conclusion: According to our results jelly fish can be an appropriate marine source for antimicrobial components. Further in vivo investigations need to be carried out on its potential application in other aspects of medicine. Manuscript profile -
Open Access Article
55 - Evaluation of Aqueous, Organic Extracts of Leaves and Roots Polygonum aviculare L. on Antibiotic-Resistant Pathogenic Bacteria Invitro and Invivo
shahrzad nassiri semnani nastaran GhasempourInroduction & Objective: Drug resistance of bacteria in infections diseases has led to more attention to the use of plants in the treatment of diseases. The aim of this study was to investigate the effects of aqueous, organic extracts of leaves and roots of Polygonu MoreInroduction & Objective: Drug resistance of bacteria in infections diseases has led to more attention to the use of plants in the treatment of diseases. The aim of this study was to investigate the effects of aqueous, organic extracts of leaves and roots of Polygonum aviculare L. on pathogenic bacteria in animal and laboratory models. Materials and Methods: In this study, after preparing aqueous, ethanolic and estonian extracts of different parts of Polygonum aviculare L. powder, the MIC and MBC of the extracts on bacteria were determined by dilution methods in broth and well diffusion in agar. In this study, an animal model was administered by peritoneal injection of 5×105 CFU/ml of bacteria and 0.5 cc of extracts with MIC concentration, number of spleen bacterial colonies after 7 days by culture on Müllerinton agar and standard counting protocol. Results: The highest levels of MIC and MBC on Staphylococcus aureus related to leafy and ethanolic leaves were 57 and 38, respectively, on Pseudomonas aeruginosa related to stem and leaf ethanol, respectively 31 and 27, on Klebsiella pneumoniae ethanolic extract of root and leaf 57 and 120 mg / ml and on Streptococcus pyogenes the estonian extract of the leaf is 227 mg / ml. In vivo, the leaf extract for Staphylococcus aureus was 7.5 × 106 CFU / ml and the ethanolic extract of the stem for Pseudomonas aeruginosa was 8 × 107 CFU / ml, for Klebsiella pneumoniae and Streptococcus pyogenes leaves and roots was 7.5 × 103 and 6.3 × 107 CFU / ml respectively. Conclusion: Polygonum aviculare L.extracts have antimicrobial effects on the studied bacteria. Manuscript profile -
Open Access Article
56 - Antimicrobial Activity medical plant extracts, Prosopis farcta L. and Datura stramonium L., against Salmonella thyphimurium isolated from poultry in Zabol
mehdi jahantigh Maryam Beigomi Zaynab Mohkami saeide saeidiThe aim of this study was to investigate the antimicrobial activity of Prosopis farcta L. and Datura stramonium L. extracts on Salmonella thyphimurium isolated from poultry in Zabol. The P. farcta and D. stramonium were collected from Collection of medicinal plants, Ins MoreThe aim of this study was to investigate the antimicrobial activity of Prosopis farcta L. and Datura stramonium L. extracts on Salmonella thyphimurium isolated from poultry in Zabol. The P. farcta and D. stramonium were collected from Collection of medicinal plants, Institute of Agricultural Research at University of Zabol. Strains of Salmonella thyphimurium were isolated from poultry droppings. Antibiotic resistance pattern was determined by Kirby Bauer method. Finally, the minimum inhibitory concentration (MIC) and minimum bactericide concentration (MBC) were determined by microdilution method. The results of this study showed that the lowest inhibitory concentration of Datura extract against Salmonella typhimurium was 3.1 ppm; While the minimum inhibitory concentration for Prosopis extract was 6.5 ppm. Also, the minimum bactericidal concentration of Datura extract was 6.25 and Prosopis extract was 12.5. The results of this study showed that Datura extract was more effective in inhibiting Salmonella bacteria than Prosopis extract. Evaluation of Pearson correlation coefficient between total phenol content, total flavonoids and MIC, and MBC showed an inverse relationship between these parameters. So that the Datura extract, which contained higher total phenol, and total flavonoid, had lower MIC and MBC and therefore was more effective in inhibiting bacteria. The results of our experiments showed that Datura and Prosopis extracts have good antimicrobial effects and can be used to treat infections caused by Salmonella typhimurium in poultry. Manuscript profile -
Open Access Article
57 - Investigating the antimicrobial properties of the medicinal plants Psidium guajava, Nerium oleander, Sinapis alba, Satureja hortensis against Salmonella typhimurium.
Betol Heydari Sadegh Reza Rouhani Nages Dahmardeh Fereshteh JavadianSalmonellosis is one of the most important common diseases of humans and animals worldwide, which causes food poisoning, gastroenteritis, enteric fever and bacteremia in humans. The purpose of this study is to investigate the antimicrobial properties of the medicinal pl MoreSalmonellosis is one of the most important common diseases of humans and animals worldwide, which causes food poisoning, gastroenteritis, enteric fever and bacteremia in humans. The purpose of this study is to investigate the antimicrobial properties of the medicinal plants Psidium guajava, Nerium oleander, Sinapis alba, Satureja hortensis on Salmonella Typhimurium.Materials and methods:Salmonella typhimurium samples were isolated from poultry feces, extracts of Psidium guajava, Nerium oleander, Sinapis alba, Satureja hortensis plants were prepared using a rotary device, and the minimum inhibitory concentration and the minimum lethal concentration were determined by the microdilution method, and the diameter of the inhibition zone was determined by the well method.Results:The results of the investigation of the inhibition halo diameter of plant extracts showed that the maximum and minimum inhibition halo diameters of P.guajava plant extract were 8 and 1 mm, and the maximum and minimum inhibition halo diameters of N.oleander flower extract were 15 and 1 mm. The maximum and minimum diameters of the inhibition zone of S.alba extract on Salmonella typhimurium were 12 and 1 mm, and the minimum and maximum diameters of the inhibition zone of S.hortensis extract on Salmonella typhimurium were 1 and 4 mm, and the results showed that that the smallest diameter of the inhibition halo was related to the salty extract.Discuss:The results of this study showed that medicinal plants have shown good inhibitory effects on Salmonella Typhimurium. Manuscript profile -
Open Access Article
58 - SYNTHESIS AND ANTIMICROBIAL ACTIVITY OF 3-(2’-n-BUTYLBENZOFURAN-3’-YL)-5-ARYL-4, 5-DIHYDRO-1H-PYRAZOLES.
RAKESH P.N.ROSHAN D.M. PUROHIT SANDIP K.MATARIYA -
Open Access Article
59 - SYNTHESIS AND ANTIMICROBIAL ACTIVITY OF 3-(2’-n-BUTYLBENZOFURAN-3’-YL)-5-ARYL-4, 5-DIHYDRO-1H-PYRAZOLES.
RAKESH P.N.ROSHAN D.M PUROHIT SANDIP K.MATARIYA -
Open Access Article
60 - Evaluation of antimicrobial effects of synthesized Zinc sulphide nanoparticles and their potentiation by the anticancer drug imatinib
Bushra Issa Kayid Al-Zuabidi Mai Yaqoob Yousif Al-Marzooq Ahmed Naser Herez Al-Musawi -
Open Access Article
61 - The inhibitory activity and phytochemical of Artemisia absinthiumL. and Oxalis corniculata L. extracts against pathogens from tomato in vitro
mohamadreza majid Khoshkholgh Pahlaviani alireza MassihaThe inhibitory activity and phytochemical of Artemisia absinthiumL. and Oxalis corniculata L. extracts against pathogens from tomato in vitroThe inhibitory activity and phytochemical of Artemisia absinthiumL. and Oxalis corniculata L. extracts against pathogens from tomato in vitro Manuscript profile -
Open Access Article
62 - Review article on the functional and antioxidant properties of ginger
Fatemeh kavian Azam RivandiAntioxidants are compounds that protect against cell damage caused by molecules called free radicals. These free radicals cause damage to DNA, cell membrane and enzymes. Various food sources rich in antioxidants, such as fruits, vegetables, spices, and types of tea... r MoreAntioxidants are compounds that protect against cell damage caused by molecules called free radicals. These free radicals cause damage to DNA, cell membrane and enzymes. Various food sources rich in antioxidants, such as fruits, vegetables, spices, and types of tea... reduce the effect of free radicals or oxidative stress. Ginger is a plant with the scientific name Zingiber officinale, whose rhizome is used as a medicine to treat a wide range of diseases in the form of fresh, powder, spice, oil and extract. Scientific studies have shown that ginger has many bioactive compounds, among these compounds we can mention 6-gingerol, which is one of the main factors responsible for the pungency of ginger and has anti-inflammatory, antioxidant, and anti-tumor properties. Ginger is related to it. Also, the use of ginger in food improves the physicochemical properties of different food products. This presented article is the result of the results of published articles related to the functional properties of ginger based on review and research studies. Manuscript profile -
Open Access Article
63 - Investigating the types of effective antimicrobial substances in food packaging and safety
Javad Badrloo Mehdi Rasouli serveh AhmadiFood packaging leads to the preservation of products in a safe environment and facilitates its transportation and storage, or by preventing chemical contamination and increasing the useful life of food products, it provides convenience to consumers. Various materials ar MoreFood packaging leads to the preservation of products in a safe environment and facilitates its transportation and storage, or by preventing chemical contamination and increasing the useful life of food products, it provides convenience to consumers. Various materials are used for food packaging, including plastic, glass, metals, paper, and their composites, and sometimes they add materials to increase the stability of the product. Due to the increasing awareness of consumers and the importance of transferring harmful substances from packaging materials to food, there is more concern about the use of any type of packaging material. The use of appropriate methods that guarantee the production, supply and consumption of healthy food along the food chain and the existence of a safe system and effective control of food will improve health and increase economic productivity. In food packaging, safety is very important. Packaging safety includes controlling the entry of harmful compounds from the packaging into the food. The interaction between food packaging and the penetration of immigrant compounds such as plasticizers, heat stabilizers, antioxidants, solvents, monomers and oligomers and pollutants resulting from the decomposition of monomers, additives and environmental pollutants changes the sensory characteristics of food. Factors affecting migration include the nature of the food, type, time and temperature of contact, the nature of the packaging material, the characteristics of the migrating material and its amount in the packaging, which are discussed in the research. Manuscript profile -
Open Access Article
64 - Functional and antioxidant properties of ginger
Azam Rivandi Fatemeh kavianAntioxidants are compounds that protect against cell damage caused by molecules called free radicals. These free radicals cause damage to DNA, cell membrane and enzymes. Various food sources rich in antioxidants such as fruits, vegetables, spices and types of tea reduce MoreAntioxidants are compounds that protect against cell damage caused by molecules called free radicals. These free radicals cause damage to DNA, cell membrane and enzymes. Various food sources rich in antioxidants such as fruits, vegetables, spices and types of tea reduce the effect of free radicals or oxidative stress. Ginger is a plant with the scientific name Zingiber officinale, whose rhizome is used as a medicine to treat a wide range of diseases in the form of fresh, powder, spice, oil and extract. Scientific studies have shown that ginger has many bioactive compounds that among these compounds, we can mention 6-gingerol, which is one of the main factors responsible for the pungency of ginger, and the anti-inflammatory, antioxidant, and antitumor properties of ginger are related to it. Also, the use of ginger in food improves the physicochemical properties of different food products. This presented article is the result of the results of published articles related to the functional properties of ginger based on review and research studies. Manuscript profile -
Open Access Article
65 - Investigation of Lactoperoxidas enzyme, functional system and its application in industries
Masoomeh Delfani Mahnaz Hashemi RavanThe production of healthy and safe food is considered as one of the most important goals of the food industry, and it is impossible to achieve this without using various processes and preservatives. However, recently, concerns about the use of chemical preservatives hav MoreThe production of healthy and safe food is considered as one of the most important goals of the food industry, and it is impossible to achieve this without using various processes and preservatives. However, recently, concerns about the use of chemical preservatives have increased, so that attention has been focused on the use of minimal or no chemical preservatives in food products. Therefore, researchers and food manufacturers are trying to produce products using natural preservatives such as antimicrobial enzymes. Lactoperoxidase, as an example of antimicrobial enzymes, which is the second most abundant natural enzyme in milk. Lactoperoxidase (LPO) is a member of a large group of mammalian hemoperoxidases, which includes myeloperoxidase (MPO), eosinophil peroxidase (EPO), and thyroid peroxidase (TPO). LPO is found in exocrine secretions, including milk. This substance is responsible for inactivating a wide range of microorganisms and is therefore an important component of the body's defense mechanism. With the help of hydrogen peroxide, it catalyzes the oxidation of halides, pseudohalides and organic aromatic molecules. This review deals with various details of this protein from its discovery to understanding its structure, function, applications and positive antimicrobial effects. Manuscript profile -
Open Access Article
66 - Investigating the use of polygalacturonase and naranjainase enzymes in the juice industry
Morva Hosseiny Mahnaz HashemiravanNowadays, the use of enzymes to improve the organoleptic characteristics of food is of interest. One of the food industries that uses enzymes to improve the sensory properties of food is the juice production industry. Among the enzymes used is the polygalacturonase enzy MoreNowadays, the use of enzymes to improve the organoleptic characteristics of food is of interest. One of the food industries that uses enzymes to improve the sensory properties of food is the juice production industry. Among the enzymes used is the polygalacturonase enzyme, which is used to clarify some fruit juices and improve the color and appearance characteristics, such as apple juice and grape juice. Polygalacturonases (E.C.3.2.1.15) known as pectinase are classified into two types of hydrolyzing polygalacturonases and polygalacturonate lyase and are among the primary enzymes used in the juice industry. This enzyme breaks the α-1→4 glycosidic bond between the units of galacturonic acid in the structure of pectin, and thus pectin precipitates out of the cell structure and is separated through filtration or centrifugation, and in this way clear fruit juice is obtained. Almost in the industry, this enzyme is used to clarify all fruit juices except citrus juice. Some fruit juices, such as orange juice and grapefruit juice, contain bitter compounds such as limonine and orange, which, if present in fruit juice, have a negative effect on its taste and create a bitter taste. Orange enzyme can be used to solve this problem. Naraninase is an enzyme that has two enzymatic activities, including alpha-L-rhamnosidase (E.C. 3.2.1.40) and beta-di-glucosidase (E.C. 3.2.1.21), which hydrolyze naraniin to rhamnose and pronin and then glucose and naraninin, without the one that leaves a bitter taste in the fruit juice. Manuscript profile -
Open Access Article
67 - Evaluation of antimicrobial effect of Zataria multiflora essential oil capsulated in chitosan biopolymer compared to free essential oil
Maasuomeh Mahdavi OurtakandEssential oils have many antimicrobial and pharmacological effects, but they are very volatile. The capsulation of drug substances in polymer nanoparticles can improve the therapeutic effects of compounds that are generally volatile. Chitosan is considered as biodegrada MoreEssential oils have many antimicrobial and pharmacological effects, but they are very volatile. The capsulation of drug substances in polymer nanoparticles can improve the therapeutic effects of compounds that are generally volatile. Chitosan is considered as biodegradable biopolymer due to better capsulation, controlled release, and low toxicity in drug delivery. The aim of this study was to investigate the antibacterial activity of Zataria multiflora essential oil capsulated to chitosan- caffeic acid nanogels in comparison to free essential oils. First, nanogel was synthesized by self-cumulative polymerization of chitosan and caffeic acid, and their shape and size were analyzed by spectrophotometric (FTIR) and scanning electron microscopy (SEM), then nanogel was Used for capsulation of Z. multiflora essential oil. Antimicrobial effect of capsulated and free essential oil was studied by disc diffusion and broth microdilution method. The results showed that the capsulated essential oil had a higher bactericidal effect on the bacteria than free essential oil. Manuscript profile -
Open Access Article
68 - The effect of chestnuts extracts on acidity, peroxide and microbial load of mayonnaise sauce
Samira Naderi alireza rahman ebrahim HoseiniIt is very important to observe hygienic principles in the production of mayonnaise, as a nutritious and popular product, as well as to prevent physical, chemical, microbial spoilage and thus prevent its quality reduction. In this study, we tried to evaluate the efficie MoreIt is very important to observe hygienic principles in the production of mayonnaise, as a nutritious and popular product, as well as to prevent physical, chemical, microbial spoilage and thus prevent its quality reduction. In this study, we tried to evaluate the efficiency of extracting aqueous extract of chestnut fruit as a natural antioxidant. For this purpose, Samples of mayonnaise with different concentrations of the extract were prepared under treatments B1 (mayonnaise + 750 ppm sodium benzoate + 750 ppm sodium sorbate), B2 (mayonnaise + 400 ppm chestnut extract + 500 ppm sodium benzoate + ppm sodium + ppm) B3 (mayonnaise +800 ppm chestnut extract +500 ppm sodium benzoate + 500 ppm sodium sorbate) and B4 (mayonnaise + 1200 ppm chestnut extract). Chemical factors (acidity and peroxide number), microbial, and sensory factors (general acceptance) were examined for the 6 months (first day, the second month, the fourth month, and the sixth month). The results showed that the extraction efficiency of the extract by the aqueous method was equal to 22.5%. According to the results, Chestnut fruit extract with benzoate and sodium sorbate significantly reduced peroxide and acid index in the samples (p<0.05). The antimicrobial effect of chestnut fruit extract is confirmed on a significant reduction in microbial population and shelf life of mayonnaise. Sample B3 was introduced as the superior treatment in this study by considering sensory factors (general acceptance). Manuscript profile -
Open Access Article
69 - Anti-yeast comparative activities of aqueous and alcoholic extracts of black tea (Camellia sinensis) and grape seed (Vitis vinifera L.) on Malassezia pachydermatis (ATCC 10231) with that of the Clotrimazole
Azam Ghrbannia Delavar niosh Qhasemi maryam onsinezhad masoud hashemi karoii issa gholampour aziziIn recent years, resistance to a variety of drugs has developed due to the indiscriminate use of antimicrobials. The aim of the present study was to compare aqueous, ethanolic and methanolic extracts of black tea(Camellia sinens) and grape seed (Vitis vinifera L.) on Ma MoreIn recent years, resistance to a variety of drugs has developed due to the indiscriminate use of antimicrobials. The aim of the present study was to compare aqueous, ethanolic and methanolic extracts of black tea(Camellia sinens) and grape seed (Vitis vinifera L.) on Malassezia pachydermatis (ATCC 10231) by disk, well, Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) methods. For black tea MIC was 25(103) µg/ml for the aqueous extract, 6250 µg/ml for the ethanolic extract and 125(102) µg/ml for the methanolic extract. MFC was 0 for the aqueous extract, 125(102) µg/ml for the ethanolic extract and 25(103) µg/ml for the methanolic extract. For grape seed MIC was 1250 µg/ml for the aqueous extract, 1250 µg/ml for the ethanolic extract and 33333 µg/ml for the methanolic extract. MFC was 16667 for the aqueous extract, 125(102) µg/ml for the ethanolic extract and 23611 µg/ml for the methanolic extract. The Clotrimazole diameter the zone of inhibition on Malassezia pachydermatis was determined between 35 and 47 mm in the disk and 40 to 49 mm in the well. In all the extracts, an increasing trend of the zone of inhibition was observed with the increase in the volume of consumed extracts. Therefore, the extracts of these two plants have an accessible and natural antibiotic effect, but compared to Clotrimazole, a smaller the zone of inhibition appeared. However, the anti- yeast activity of grape seed extracts is more than that of black tea. Manuscript profile -
Open Access Article
70 - Study of Antibacterial Synergistic Effect of Origanum vulgare L. Essential Oil and Silver Nanoparticles
Nahid mohammadzadeh Maasuomeh Mahdavi-Ourtakand Sahar Honarmand JahromiThe increasing use of antibiotics and the prevalence of resistant strains have necessitated the use of new antimicrobial drugs. Combination therapy is an effective strategy to combat antibacterial resistance. The aim of this study was to investigate the antibacterial sy MoreThe increasing use of antibiotics and the prevalence of resistant strains have necessitated the use of new antimicrobial drugs. Combination therapy is an effective strategy to combat antibacterial resistance. The aim of this study was to investigate the antibacterial synergistic effect of Origanum vulgare essential oil and silver nanoparticles on gram-positive and gram-negative bacteria. In this study, O. vulgare essential oil was extracted from the plant branch. A colloidal solution of silver nanoparticles with an average particle diameter of 68 nm was purchased. The antibacterial effect of O. vulgare essential oil and silver nanoparticles was investigated alone and then in combination by broth microdilution method and their minimum inhibitory concentration (MIC) against gram-positive and gram-negative bacteria was determined. The bacteria studied were Escherichia coli, Salmonella enterica, Bacillus subtilis and Staphylococcus aureus. Based on the obtained results, the synergistic effect of O. vulgare essential oil and silver nanoparticles had significant antimicrobial effects on all studied bacteria. The results showed that the synergistic effect of O. vulgare essential oil and silver nanoparticles was effective in inhibiting and killing the studied bacteria. And they can be useful in developing new drugs against these bacteria. Manuscript profile -
Open Access Article
71 - Phytochemical investigation of the extract of Astragalus tragacantha collected from the vegetative areas of Meshkin Shahr and its antimicrobial effects on some common oral and dental bacteria
Hojjat Eghbal Mehdi Ahmadi SabeghIntroduction and purpose: Recently, due to the increasing resistance of microorganisms to common antibiotics, the use of phenolic and antimicrobial compounds in medicinal plants has received special attention.Materials and methods: Gon extract was extracted by maceratio MoreIntroduction and purpose: Recently, due to the increasing resistance of microorganisms to common antibiotics, the use of phenolic and antimicrobial compounds in medicinal plants has received special attention.Materials and methods: Gon extract was extracted by maceration method and their phenolic compounds were identified by HPLC, the antimicrobial effect of the extract was investigated in concentrations (25, 50 and 100 µg/ml). These bacteria included common microbes and fungi, including Streptococcus mutans, Streptococcus sanguinis, Streptococcus salivaris, Streptococcus subrinus, Escherichia coli, Icnella cordens, Pseudomonas aeruginosa and Klebsiella pneumoniae. Disc diffusion and MIC methods were used to investigate the antimicrobial activity of the extracts.Findings: In the investigation of the effect of the desired extract on bacteria, it was found that the medicinal plant Gon has the least inhibitory effect against Streptococcus sanguinis bacteria and the most inhibitory effect on Candida glabrata fungus. The results of the MIC test also confirm these effectsConclusion: According to the phytochemical results obtained from the extract of this research, it can be stated that the studied medicinal plant has antibacterial phenolic compounds. Therefore, the results of the research showed that the extract of the medicinal plant Goon has a suitable anti-microbial effect against the bacteria causing oral and dental infections, and it was expressed as an alternative to the standard drugs chlorhexidine and nystatin. Manuscript profile -
Open Access Article
72 - A review of the antimicrobial and antibacterial effects of silver nanoparticles and silver composites
Zahra Javanshir Maryam MohammadpoorToday, nanoparticles include a wide range of particles with unique properties. Due to their small size and unique properties, these particles can be used in various sciences. Nanoscale biological compounds have unique physical-chemical properties that are In recent year MoreToday, nanoparticles include a wide range of particles with unique properties. Due to their small size and unique properties, these particles can be used in various sciences. Nanoscale biological compounds have unique physical-chemical properties that are In recent years, recently, the efficacy of antimicrobial nanoparticles, especially silver nanoparticles and their antimicrobial activity, has been the focus of researchers and researches have been conducted. In this study, recent advances and research results of researchers in the use of antibacterial and antiseptic properties of silver nanoparticles, silver and silver composites have been reviewed.The search was done on SID and Google Scholar sites. We did not have a time limit for the search, and all the articles that met the entry criteria were included in the study until March 2018. Among the articles found, some of the articles with the conditions including the complete article have antibacterial and anti-infective properties. 10 articles were evaluated. All the articles have anti-bacterial and anti-infective properties of Silver nanoparticles, silver and silver composites have been in different levels. Manuscript profile -
Open Access Article
73 - Effect of Heracleum persicum (Golpar) essential oil on some microbial, chemical and sensory characteristics of heat treated Dough
A.R. Yousefi H. Sheikhloie Bonab J. Seyfi Hachesou M. HatamiNowadays, many studies have been conducted to substitute the chemical preservatives with naturally occurring compounds, including plant essential oils. In this research, Golpar essential oil was added to Dough samples at concentrations of 0 (control), 100, 250, 500 and MoreNowadays, many studies have been conducted to substitute the chemical preservatives with naturally occurring compounds, including plant essential oils. In this research, Golpar essential oil was added to Dough samples at concentrations of 0 (control), 100, 250, 500 and 1000 ml/l and after packaging, were stored at refrigerator for 40 days. The samples were subjected to microbial (enumeration of yeasts and molds, yoghurt starter bacteria and coliforms), chemical (acidity, pH, fat and solids-non-fat) as well as sensory characteristics (taste and odor) assays. Results showed that with the increasing of the essence concentration, the populations of yeast and mold were reduced (P<0.05), however, the effect of Golpar essential oil on starter bacteria was insignificant. The number of starter bacteria increased towards day 10, followed by a decrease until the end of storage time. Although during the storage period, acidity and pH were increased and decreased, respectively, the effect of essential oil on fat content and solids-non-fat was not significant. Sensory evaluations in terms of taste and odor revealed that the treated samples had significant (p< 0.05) difference in comparison with control groups. Besides, the samples containing 250 µl/l gained the highest score. It was concluded that Golpar essential oil can be efficiently used in Dough formula as a good natural preservative and flavoring agent. Manuscript profile -
Open Access Article
74 - Physico-mechanical and antimicrobial properties of edible film incorporated with Caryophillum aromaticcus essential oil (CAO) from Caspian White fish (Rutilus frisii Kutum) scale
A. Mahjoorian m.R. Saeedi Asl M. Rezaei A. Mortazavi P. AriayeByproducts from the processing of fish are a good source of collagen. Biodegradable protein-based film was made by incorporating clove essential oil (CAO) into Caspian white fish scale gelatin (CWFSG) at level of 0, 0.5%, 1% and 1.5% (weight/ protein weight ratio) and a MoreByproducts from the processing of fish are a good source of collagen. Biodegradable protein-based film was made by incorporating clove essential oil (CAO) into Caspian white fish scale gelatin (CWFSG) at level of 0, 0.5%, 1% and 1.5% (weight/ protein weight ratio) and antimicrobial, physical and mechanical properties of films including tensile strength (T.S), elongation at break (EAB%), moisture%, water vapor permeability (WVP), contact angle, solubility, surface color properties, and scanning electron microscopy (SEM) were investigated. Gelatin-based films enriched with different concentrations of CAO were prepared and examined. Incorporation of CAO at concentrations of 0-1.5% resulted in the decreasing in tensile strength (TS) of the films. Water vapor permeability (WVP) was increased in the film added with CAO at a level higher than 0.5% (P<0.05); However, film solubility and L*value decreased. Moreover, when CAO was incorporated the films showed the lowered light transmission in the visible range. Films incorporated with CAO had an inhibitory effect in a concentration-dependent manner against Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli. SEM images revealed the presence of micro-pores in the essential oil incorporated films, which contributed to the physical properties of the films. Gelatin films incorporated with CAO can be used as an active packaging, but the properties must be modified. Gelatine film containing 1% CAO had the best physical, mechanical and antimicrobial properties. Manuscript profile -
Open Access Article
75 - Antioxidant properties and antimicrobial activity of Prosopis farcta root extract on foodborne bacteria
H. Aziznia J. Keramat صبیحه Soleimanian zadPlants are valuable sources of different antioxidant and antimicrobial compounds. The composition and structure of the functional groups of essential oils and extracts play an important role in their antioxidant and antimicrobial activity. Prosopis farcta is a spice of MorePlants are valuable sources of different antioxidant and antimicrobial compounds. The composition and structure of the functional groups of essential oils and extracts play an important role in their antioxidant and antimicrobial activity. Prosopis farcta is a spice of Leguminosae family and Mimosoideae subfamily, an aborigine to the dry and semi-dried regions of Asia, Africa and America. In this study, the root extract of Prosopis farcta prepared by the soxhlet extractor and ethanol 70% solvent. The extraction yield was estimated at 1.85%. Total phenol and flavonoids compounds were calculated 178.14 ± 0.17 mg Gallic acid/g and 94.33 ± 0.77 mg Quercetin/g, respectively. Phenolic compounds comprised 11.98% of the total chemical composition of the extract. IC50 for root extract and BHT (synthetic antioxidant) was determined 2.45 μg/ml and 1.98 μg/ml, respectively. In the β-carotene-linoleic acid system, the average antioxidant activity reported 39.25% for root extract and 57.13% for the BHT. Among 250, 500 and 1000 ppm concentrations of the extract, 1000 ppm showed the best antioxidant effect in soybean oil for 12 days storage at 50°C. Staphylococcus aureus (with MIC 200 µg/ml and MBC 350 µg/ml) showed the most sensitivity in comparison with the two gram-negative bacteria, Salmonella Typhimurium and Escherichia coli. The results of this study showed that the hydro-ethanolic root extract of Prosopis farcta had suitable antimicrobial and antioxidant activity. Manuscript profile -
Open Access Article
76 - Evaluation of antioxidant and antimicrobial properties of )Echinacea purpurea( extract and identification of extract compounds by gas chromatography-mass spectrometry (GC-MS)
M. Ghaedan N. Zamindar M. Goli N. Ghasemi SeproThis study aimed to investigate the antioxidant and antimicrobial properties of Echinacea purpurea, which is used as an antiseptic, immune system booster, antimicrobial, antioxidant and anti-poisoning herbaceous plant. Antioxidant and antimicrobial properties, MoreThis study aimed to investigate the antioxidant and antimicrobial properties of Echinacea purpurea, which is used as an antiseptic, immune system booster, antimicrobial, antioxidant and anti-poisoning herbaceous plant. Antioxidant and antimicrobial properties, total phenolic compounds and DPPH radical scavenging of the extract were measured. Antimicrobial properties of the extract were assessed using well-diffusion method. The components of the extract were identified using gas chromatography-mass spectrometry (GC MS). The data were analyzed using SPSS and Duncan's statistical test. The plant extract showed high level of phenolic compounds. The DPPH radical scavenging of the extract was tested at concentrations of 0.22, 0.25, 0.27, 0.31, 0.35, 0.41, 0.50 g/ml. The concentration of IC50 indicated sufficient inhibitory potential of the extract. The diameter (means ± SD) of the growth inhibition zone for Staphylococcus aureus, Escherichia coli and Aspergillus niger was estimated at 4.67 ± 1.24, 3.11 ± 0.84 and 1.78 ± 0.89 mm, respectively. Based on well-diffusion assay, S. aureus was found more sensitive than E. coli, and E. coli was more sensitive than A. niger. Using the gas chromatography-mass spectrometry, 81.38% of the total extract compounds were identified. Important components of the extract consisted of germacrene D (21.67%), paracetamol (5.53%), Bernoulli (4.58%), respectively. The results showed that Echinacea extract had excellent antioxidant and antimicrobial effects. Manuscript profile -
Open Access Article
77 - Potassium sorbate reduction in sponge cake with herbal extracts and evaluation of its properties
A. Badali A. JavadiDue to the adverse effects of chemical preservatives and the negative attitude of consumers, the use of herbal essential oils as natural preservatives has attracted particular attention. In this regard, 11 cake samples containing 0 to 1000 mg/kg extracts of ginger, cinn MoreDue to the adverse effects of chemical preservatives and the negative attitude of consumers, the use of herbal essential oils as natural preservatives has attracted particular attention. In this regard, 11 cake samples containing 0 to 1000 mg/kg extracts of ginger, cinnamon and cardamom solely or in combination were prepared and compared during 3 storage time (production day (day 1), day 30 and day 60) with two control samples with (positive control) and without (negative control) potassium sorbate in terms of chemical, microbial and sensory properties. Results showed that in all samples pH decreased with time and acidic and peroxide values increased (P< 0.05). Microbial examination of the samples showed the amount of mold and yeast increased during storage time (P< 0.05). In terms of sensory properties, all samples were highly scored on the first day of production, however the sensory scores decreased during storage period. According to the results, using an equal combination of cardamom, ginger and cinnamon extracts (sample 3 containing cardamom and cinnamon and sample 4 with equal content of three extracts) were able to increase the shelf life and maintain the sensory properties of the cakes without the use of chemical preservatives. Manuscript profile -
Open Access Article
78 - Effect of Oliveria Decumbens essential oils on microbial characteristics of hamburger
Z. Ghorbani N. Zamindar M. Jelvan M. Golabadi< p >The use of herbal essential oils can inhibit bacterial growth and proliferation. In this investigation, the chemical composition and antimicrobial effects of essential oils of Oliveria decumbens on culture media and hamburger have been studied. GC/M More< p >The use of herbal essential oils can inhibit bacterial growth and proliferation. In this investigation, the chemical composition and antimicrobial effects of essential oils of Oliveria decumbens on culture media and hamburger have been studied. GC/MS analysis identified 12 components in the essential oils of Oliveria decumbens. The antimicrobial activity of the essential oils was investigated against Staphylococcus aureus and Escherichia coli using disc diffusion and well plate method. The experiment was a factorial form of a completely randomized design with 3 levels in essential oil concentration (0, 0.32, and 1.25 µl/g) in 4 periods (1, 20, 40, and 60 days) with 3 replications on S. aureus, E. coli, mold, yeast and total count in hamburger. The most important identified compounds in the essential oils of Oliveria decumbens were phenolic and aldehyde compounds. Minimum inhibition concentrations of Oliveria decumbens essential oils against S. aureus and E. coli were 0.32 µl/ml and 0.625 µl/ml, respectively. Besides, the number of microorganisms decreased with increasing concentrations of essential oils of Oliveria decumbens. There was no significant difference between the control and concentrations of 0.32 µl/g in E. coli count but in all types of microorganisms, the highest decrease was observed in the concentration of 1.25 µl/g (p < /em> Manuscript profile -
Open Access Article
79 - Identification of enterocins and in vitro characterization of their antimicrobial and anticancer activity in Enterococcus strains isolated from traditional fermented products
Faezeh Salek Hamid Mirzaei Jalil Khandaghi Afshin Javadi Yosef NamiReplacing naturally occurring bioactive substances, such as microbial metabolites, with synthetic preservative compounds, and getting access to their beneficial properties, is expanding every day. Meanwhile, the focus of many researchers' attention has been on bacterioc MoreReplacing naturally occurring bioactive substances, such as microbial metabolites, with synthetic preservative compounds, and getting access to their beneficial properties, is expanding every day. Meanwhile, the focus of many researchers' attention has been on bacteriocins, which have multiple physiological functions, including antibacterial and anticancer properties. In this study, the enterocin production genes of Enterococcus isolates from two fermented dairy products (Motal cheese and Tarkhineh) were tracked using a molecular method. The kinetics of bacteriocin-like substance production, as well as its stability (at high temperatures, different pH levels, and under different enzyme influences), were investigated. The antagonistic (well diffusion method) and anticancer (with MTT colorimetric technique) characteristics of enterocins generated by these isolates were also investigated in two human cancer cell lines. The results of the PCR test showed that isolates of Enterococcus faecium AUT-7KB and Enterococcus faecalis KUMS-T48 contained all three studied enterocin production genes, and the bacteriocin-like metabolites of these strains had strong antagonistic effects on Listeria monocytogenes and Bacillus cereus. These metabolites had high stability at different pH and became ineffective under the influence of protease enzymes (trypsin and pepsin), which indicated their protein nature. Additionally, metabolites of the KUMS-T48 isolate showed strong cytotoxic effects on two cancer cell lines, AGS and HT-29. According to the findings of the current research, enterocins of the E. faecalis KUMS-T48 strain are suggested as a suitable candidate for further anti-tumor studies, such as in vivo studies, after complete investigations of safety aspects. Manuscript profile -
Open Access Article
80 - Investigation of the physico-chemical and antimicrobial properties of propolis extract as a natural food preservative
ّF. Ghavidel H. Jafarizadeh-Malmiri A. Javadi N. AnarjanAs a natural bioproduct obtained from beekeeping, propolis with its antimicrobial and antioxidative properties can be used in food production. The purpose of this study is to evaluate the application of propolis as a preservative in food. After preparation of propolis, MoreAs a natural bioproduct obtained from beekeeping, propolis with its antimicrobial and antioxidative properties can be used in food production. The purpose of this study is to evaluate the application of propolis as a preservative in food. After preparation of propolis, the physicochemical properties of raw propolis and extract were evaluated with three repetitions. the results showed that amount of waxes, moisture, fat, protein, ash and pH of raw propolis were 35.10±0.89%, 4.2±0.14%, 64.78± 0.22%, 2.30± 0.13%, 1.25± 0.25%, 4.89 ± 0.06 respectively. Obtained results of propolis extract analysis indicated that antioxidant activity, turbidity, pH, brix, total phenolic and flavonoids content of the prepared propolis ethanolic extract were 94.5±0.02%, 1.091±0.03%a.u., 5.9±0/12 and 18.5±0.07 Bx°, 91.12±0.09 mg/g (gallic acid) and 87.94±0.03 mg/g (quercetin), respectively. The extracted propolis extract was evaluated by Gas chromatography / mass spectrometry to identify chemical compounds. The results showed that the main constituents of propolis extract were included Pinostrobinchalcone (22.90%), Pinocembrin (6.14%), 2,1,3-Benzothiadiazole (5.76%), Tectochrycin (4.83%), Phenethylalcohol (4.25%), Oleic acid (3.10%). Evaluation of antimicrobial activity of propolis extract showed that the prepared extract had high antibacterial activity against Staphylococcus aureus while it was ineffective on Escherichia coli. The extract also inhibited the growth of Aspergillus fluvus fungus, unlike the control sample, so that its mycelia diameter was reached 7 mm after 8 days incubation. In general, the results showed that the produced propolis extract can be used as an effective natural preservative in foods. Manuscript profile -
Open Access Article
81 - Antimicrobial effects of lactic acid bacteria with probiotic ability isolated from traditional yogurts on foodborne pathogenic microorganisms
M. Sadeghi A. Tarinejad M. A. Hejazi Y. NamiThe growth of contaminating microorganisms in food products causes serious diseases in humans, and the use of lactic acid bacteria to control these contaminants is a promising and developing method. In this study, lactic acid bacteria were isolated from traditional yogu MoreThe growth of contaminating microorganisms in food products causes serious diseases in humans, and the use of lactic acid bacteria to control these contaminants is a promising and developing method. In this study, lactic acid bacteria were isolated from traditional yogurts and a total of 140 isolates were isolated. The probiotic potential of these lactic acid bacteria including resistance to acidic conditions, resistance to bile salts and sensitivity to ten important antibiotics were investigated and then bacteria with probiotic potential were used to inhibit food pathogens. The measured halo diameters of the bacterial medium were measured and recorded, and the antimicrobial properties of the strains were compared. ARDRA technique was also used to identify the strains. The results showed that all strains have excellent tolerance to pH 2.5 and bile salt of 0.3% and isolates Numbers 12, 100 and 102 are recognized as the most resistant strains against studied food pathogens. Findings from this study showed that yogurt samples are an important source for the production of bacteria with probiotic potential with antimicrobial activity and can be used against food pathogens. Manuscript profile -
Open Access Article
82 - Evaluation of antimicrobial effects of aqueous and alcoholic extracts of Spirulina platensis in UF white cheese
E. Zanganeh H. Mirzaei S.M. Jafari M. R. Afshar Mogaddam َA. JavadiOne of the most widely used cheeses in Iran is UF white cheese. The presence of nutrients in different types of cheese, make that susceptible to the growth of various microorganisms. Therefore, the use of preservatives to maintain the quality of this high-consumption da MoreOne of the most widely used cheeses in Iran is UF white cheese. The presence of nutrients in different types of cheese, make that susceptible to the growth of various microorganisms. Therefore, the use of preservatives to maintain the quality of this high-consumption dairy product is inevitable. In this study, the effect of concentrations of 0.3 and 0.5% aqueous and methanolic extracts of Spirulina platensis on growth inhibition of Listeria monocytogenes, Staphylococcus aureus and Escherichia coli and also the effect of these extracts in preventing the growth of A.flavus in the UF white cheese has been investigated during 60 days of storage of the product in the refrigerator. In addition, the effect of extracts on texture, taste, color and overall acceptance of the product was evaluated. According to the results, a significant inhibitory effect (p≤0.05) of concentrations of 0.3 and 0.5 aqueous and methanolic extracts was observed on Listeria monocytogenes and Escherichia coli from the 15th day of the storage period and on Staphylococcus aureus from the 30th day of the storage period. The antibacterial effect of 0.5 concentration of methanolic extract was significantly higher than other treatments (p≤0.05). All treatments had a significant antifungal effect on Aspergillus flavus strains (p≤0.05). According to the results of the present research, the used extracts did not have a significant adverse effect on the sensory characteristics of the cheese samples, although the concentration of 0.5 methanolic extract insignificantly decreased the quality of the sensory characteristics of the cheese samples. In general, it can be said that concentrations of 0.5 aqueous extract and 0.3 concentration of methanolic extract of Spirulina platensis can be used as natural preservatives in UF white cheese. Manuscript profile -
Open Access Article
83 - Investigation some of the chemical and antimicrobial properties of laurus nobilis essential oil
Farideh Ghaderi Amir Shakerian zohreh Mashak Ebrahim Rahimi Mahdi JafariDry leaf of trees, their powder and essential oil are used as flavoring agents in the food industry, and due to their antimicrobial and antioxidant properties, they increases the shelf life of food. The aim of this research is to investigate the chemical and antimicrobi MoreDry leaf of trees, their powder and essential oil are used as flavoring agents in the food industry, and due to their antimicrobial and antioxidant properties, they increases the shelf life of food. The aim of this research is to investigate the chemical and antimicrobial properties of bay leaf essential oil. Extraction of essential oils was done by clevenger method. The results showed that the amount of carbohydrate, total fat and protein in the leaves were 2.93, 6.51 and 6.33 grams per 100 grams of dry matter, respectively, and antioxidant activity, total phenol and flavonoid content of the prepared essential oil, respectively, with IC50 of the number is 0.91 micrograms per milliliter, 0.65 m/g of gallic acid and 0.07 m/g of quercetin. The results of GC-MS showed that the main components of the essential oil include cineole (33.63 %), camphene (19.76 %), eugenol (11.73 %), terpineol (5.30 %), alphapinene (45.45 %.), Sabnin (3.42%), alpha-terpineol (0.45%) and methyl eugenol (0.33%). The evaluation of the antimicrobial activity of laurus nobilis essential oil indicates that the produced essential oil had antimicrobial activity against Staphylococcus aureus with a halo diameter of 14.60 ± 0.47 mm, while it was effective against Escherichia coli and Salmonella bacteria, respectively. It had less effect with halo diameter of 10.60 ± 0.47 and 9.60 ± 0.47 mm. Manuscript profile -
Open Access Article
84 - Investigating the antimicrobial properties of edible film of polylactic acid-Iranian tragacanth gum along with the essential oil of Zygophyllum eichwaldi on the microbial spoilage of ostrich thigh meat at refrigerator temperature
Azin Kiakojori peiman ariaii Roya Bagheri Mahro EsmaeiliThe present study was conducted with the aim of investigating the effects of PLA- Iranian tragacanth gum composite film with Zygophyllum eichwaldi essential oil on the microbial characteristics of ostrich thigh meat stored at 4°C. For this purpose, first, 5 film tre MoreThe present study was conducted with the aim of investigating the effects of PLA- Iranian tragacanth gum composite film with Zygophyllum eichwaldi essential oil on the microbial characteristics of ostrich thigh meat stored at 4°C. For this purpose, first, 5 film treatments including: PLA, PLA+ gum and PLA+ gum with concentrations of 0.5%, 1.5% and 1.5% essential oil to determine the microbial properties of the film and then the effect of the mentioned films along with the control treatment (without film) was evaluated on the microbial factor (total bacterial count, psychrophilic bacteria, Enterobacteriaceae and lactic acid) in the ostrich fillet samples tested on days 0, 4, 8, 12 and 16 and the resulting data were SPSS software was analyzed. According to the results, adding essential oil to the film increased the antimicrobial activity of the films, and with increasing concentration, better results were observed (P<0.05) and the antimicrobial activity against gram-positive bacteria was higher than gram-negative bacteria. The results of the second part of this study showed that the samples packed with composite films containing essential oil showed lower (P<0.05) microbial changes than the control samples without essential oil during the study, and the film containing 0.5 and 1% of essential oil had the best effects compared to other groups (P<0.05). Therefore, the use of PLA- Iranian tragacanth gum film containing Zygophyllum eichwaldi essential oil in the packaging of ostrich meat has a very favorable effect in controlling the microbial characteristics effective in meat spoilage and increases its shelf life. Manuscript profile -
Open Access Article
85 - A comparative study of the antimicrobial properties of nisin and natamycin in pasteurized liquid egg white and increasing its shelf life
Elham Yaghma Mohammad Ali Torbati Afshin JavadiThis study aims to compare the antimicrobial properties of nisin and natamycin in pasteurized liquid egg whites. After breaking and processing the eggs and performing operations such as filtration and homogenization, the egg white liquid undergoes pasteurization at 56-5 MoreThis study aims to compare the antimicrobial properties of nisin and natamycin in pasteurized liquid egg whites. After breaking and processing the eggs and performing operations such as filtration and homogenization, the egg white liquid undergoes pasteurization at 56-57 °C for 10 minutes with a flow rate of 1800 m3/s. Following pasteurization, the liquid is mixed with 0.08% and 0.1% solutions of nisin and natamycin in packaging tanks. Microbial tests are conducted at 0, 7, 14, and 30 days of storage. Based on the results, the antimicrobial effect of both natural preservatives nisin and natamycin in the microbial tests of total count, coliform, mold, and yeast have a significant difference at the probability level of 5%. In the total count test, 0.1% nisin on the seventh day exhibits the lowest total count (1.71 log CFU/gr), while the control sample on the 30th day shows the highest (4/49 log CFU/gr). For natamycin, 0.1% on the seventh day and 0.08% on the seventh day have the lowest counts (2.63 log CFU/gr and 2.67 log CFU/gr) respectively, and the control sample on the 30th day has the highest (4.5 log CFU/gr). No microorganism growth is observed in samples containing nisin and natamycin for coliform, mold, yeast, Escherichia coli, Staphylococcus aureus, and salmonella tests. Nisin demonstrates a more intense reduction in total count compared to natamycin, indicating superior antimicrobial properties and increased shelf life over time. Manuscript profile -
Open Access Article
86 - Antimicrobial effect of essential oils: a systematic review
احسان Sadeghi عبداله Dargahi احمد Mohammadi فاطمه Asadi سمیه SahraeeRegarding the harmful effects of chemical foods preservatives on human body, it is crucial to find out safe antimicrobials among essential oils and herbs. This study aimed to address the effects of different essential oils on various bacterial species through a systemat MoreRegarding the harmful effects of chemical foods preservatives on human body, it is crucial to find out safe antimicrobials among essential oils and herbs. This study aimed to address the effects of different essential oils on various bacterial species through a systematic review. A wide range of published papers in national and international data bases have been searched for the relevant articles. For this reason, the keywords used in searching were: "essential oils in food", "antimicrobial effect" and "vegetable oils". Among 462 retrieved articles, 76 papers were selected for further reviewing based on their title and abstracts. Based on results, the antimicrobial effects of different essential oils on pathogenic and spoilage organisms were compared. Moreover, the most effective as well as the least effective essential oils on microbial growth were identified. It was concluded that essential oils are more effective on gram positive bacteria rather than gram negatives. Besides, it was evident that some essential oils negatively affected the useful organisms such as lactobacilli. Manuscript profile -
Open Access Article
87 - Sumac (Rhus coriaria L.) essential oil composition and its antimicrobial effect on Salmonella typhimurium
بهراد Radmehr کاوه Khamda افشین Rajabi KhoramiConsumers' interest over natural preservatives and their application as food ingredients is growing, which encourage complementary researches on various natural antimicrobials such as plant essential oils. Sumac (Rhus coriaria L.), is a plant that is used as a flavourin MoreConsumers' interest over natural preservatives and their application as food ingredients is growing, which encourage complementary researches on various natural antimicrobials such as plant essential oils. Sumac (Rhus coriaria L.), is a plant that is used as a flavouring agent in Iranian traditional foods. Therefore, in this study Sumac essential oil composition and antimicrobial activity was evaluated. Sumac ssential oil was extracted with distillation and analyzed by gas chromatography method. Antimicrobial effect of Sumac essential oil against Salmonella typhimurium, that is considers as one of important pathogen in food, was evaluated. Antimicrobial effect of essential oil was evaluated by measuring turbidity as a result of bacterial growth at a broth media by Bioscreen C instrument at 35°C. Result showed that the most important fraction of essential oil that may have antimicrobial effect is Caryophyllen. Although Sumac essential oil had bacteriostatic effect at low concentration of 30 ppm, it showed bactericidal activity at high concentration of 1000 ppm. It could be concluded that, Sumac essential oil by its antimicrobial fractions can be used to control the growth of Salmonella typhimurium in food stuff. Manuscript profile -
Open Access Article
88 - Effect of Aloe vera gel on antimicrobial and sensory properties of ultra-filtered white cheese
K. Sajadi S. BahramianAloe vera gel contains a blend of carbohydrates, enzymes, vitamins and minerals, and has antimicrobial and anti-oxidant properties. In this study, after extraction and homogenization of Aloe vera gel, the effect of various concentrations (0.5, 1, 2, 5, 10 and 15%) of th MoreAloe vera gel contains a blend of carbohydrates, enzymes, vitamins and minerals, and has antimicrobial and anti-oxidant properties. In this study, after extraction and homogenization of Aloe vera gel, the effect of various concentrations (0.5, 1, 2, 5, 10 and 15%) of the gel was investigated on flavor microbial flora (total microbial count and mesophilic lactic acid bacteria) and also its inhibitory effect on Penicillium citrinum (PTCC 5304) in cheese. Results of sensory evaluation showed that cheeses produced with the concentrations of 0.5 and 1% gel had the highest acceptance. Moreover, it was revealed that in the control sample the number of total count and lactic acid bacteria increased in from 1 to 3 months of storage; meanwhile in the gel-containing samples the microbial populations reduced during the same period.In addition, Aloe vera gel at the concentration of 15% caused 37.3% inhibition of P. citrinum in. It was concluded that some concentration of Aloe vera gel could retard the growth of P. citrinum without sensory defects. Manuscript profile -
Open Access Article
89 - Prevalence and antimicrobial susceptibility of Listeria monocytogenes in Koozeh cheeses of Urmia retails
B. Abbasinejad مسلم Neyriz-Nagadehi Nagi Taher Talatappeh Koozeh cheese is of the local traditional cheeses of Azerbaijan and Kurdistan areas of Iran, and this cheese has high consumption among the people of these regions. Listeria monocytogenes is a food borne pathogen, and because of its growth at refrigeration More Koozeh cheese is of the local traditional cheeses of Azerbaijan and Kurdistan areas of Iran, and this cheese has high consumption among the people of these regions. Listeria monocytogenes is a food borne pathogen, and because of its growth at refrigeration temperatures has great concern in public health. In the present research, prevalence rate of L. monocytogenes in Koozeh cheeses of Urmia retails was surveyed. Moreover, antimicrobial susceptibility of the isolates was studied. One hundred samples of Koozeh cheese were collected randomly from Urmia retails in the spring of 2013. Samples were cultured in L-PALCAM Listeria selective enrichment broth with supplement. The enriched samples were transferred to PALCAM Listeria selective agar with antibiotics. For the identification of Listeria species, specific biochemical tests were performed. Antibiotic susceptibility of L. monocytogenes isolates was determined by disk diffusion method using penicillin G, chloramphenicol, ceftriaxone, erythromycin, tetracycline, and gentamicin. Among the 100 tested samples, L. monocytogenes was isolated from 3 of the samples. Antibiogram results showed that all L. monocytogenes isolates were susceptible to the tested antibiotics. However, tetracycline, penicillin and erythromycin were shown larger diameter of inhibition zone than the others. Although the prevalence rate of L. monocytogenes in Koozeh cheeses was low, its capability to growth in refrigerator conditions along with the pathogenicity of the bacterium to humans, hygienic production and distribution of these cheeses are recommended. Manuscript profile -
Open Access Article
90 - Comparison of antibacterial activity of four cultivars of olive (Olea europaea) leaf extract on Bacillus cereus
مریم Abbasvali محمود Esmaeili Koutamehr حمدالله Moshtaghi M.H EskandariIn recent years, plant extracts have been used as antimicrobial agents. One of these extracts is olive leaf extract, which has antimicrobial properties due to its phenolic compounds. In current study the leaf extract of four olive (Olea europaea) cultivars (Shiraz, Zard MoreIn recent years, plant extracts have been used as antimicrobial agents. One of these extracts is olive leaf extract, which has antimicrobial properties due to its phenolic compounds. In current study the leaf extract of four olive (Olea europaea) cultivars (Shiraz, Zard, Roghani and Dezfool) was extracted by different solvents (acetone, methanol and ethanol) using microwave-assisted extraction method. Then the antibacterial activity of the extracts was assessed on Bacillus cereus. The antibacterial activity of the extracts was determined using the standard and micro broth-dilution methods. All experiments were carried out in triplicate. Minimum inhibitory concentration (MIC) of eleven extracts was estimated as 20 mg/ml, and only MIC of the methanolic extract of Roghani cultivar was 10 mg/ml. Bacterial growth curve of B. cereus in the presence of olive leaf extracts was drawn after 24 h incubation at 37 °C and the mean percentage of growth inhibition of each extract was measured after 24 hours. The results showed that in comparison with the other cultivars, the methanolic extract of Roghani cultivar with the percentage of growth inhibition of 91.3% and 87.8% (in the concentrations of 10 and 20 mg/ml, respectively), demonstrated stronger antibacterial effect on B. cereus. This study indicated that olive leaf extract from inexpensive agricultural waste might be a valuable bioactive source with antibacterial activity, and seem to be applicable as a safe food additive. Manuscript profile -
Open Access Article
91 - Antimicrobial properties of clove essential oil on raw hamburger during storage in freezer
S.E Hoseini SH Shabani فاطمه Delfan Azari Providing safe foods resistant to pathogens as well as replacing chemical preservatives with natural compounds including essential oils has attracted great attention in current studies. The purpose of this study was to investigate the antimicrobial properti More Providing safe foods resistant to pathogens as well as replacing chemical preservatives with natural compounds including essential oils has attracted great attention in current studies. The purpose of this study was to investigate the antimicrobial properties of clove’s essential oil on hamburger. For this reason, clove essential oil was extracted by Clevenger apparatus method. Minimum Inhibitory Concentration (MIC) of Staphylococcus aureus, Escherichia coli, Candida albicans and Aspergillus niger was measured. The antimicrobial effect of the essence in the concentrations of 0.0015%, 0.01%, 0.1% and 0.15% was examined on hamburger. The samples were examined at 0, 7, 30, 60 and 90 days of cold storage. Sensory characteristics of the samples were assessed through Hedonic method. MICs resulted from the test for S. aureus, E. coli, C. albicans and A. niger were 0.1%, 0.065%, 0.03% and 0.01% (V/V), respectively. The results of microbial examinations indicated that with the increasing of the concentration of clove oil essence and also with the progression of storage time, the microbial load was gradually decreased. According to the sensory assays conducted by the consumers, low concentrations of 0.0015% and maximum of 0.01% were found desirable. Manuscript profile -
Open Access Article
92 - Effect of active vitamin D3 on the expression of antimicrobial peptide genes in experimental calf pneumonia
Parisa asgharpour Zohreh Eftekhari Mohammad Gholi Nadealian Gholamreza Nikhbakht Borujeni Mohammad Reza Mokhber DezfouliThe outbreak of calf pneumonia in herds occurs due to different pathogens. Vitamin D3 displays a regulatory effect on different cells, especially its pro-differential biological function and stimulates antimicrobial peptides. In the present study, the effects of vitamin MoreThe outbreak of calf pneumonia in herds occurs due to different pathogens. Vitamin D3 displays a regulatory effect on different cells, especially its pro-differential biological function and stimulates antimicrobial peptides. In the present study, the effects of vitamin D3 on the expression of antimicrobial peptides in experimental pasteurellosis in calves were assessed. Ten Holstein calves (2-4 months old) were randomly divided into two groups. Prepared Pasteurella multocida (3×109 CFU/mL) was inoculated in the trachea of all calves and Vitamin D3 was injected only in the treatment group following confirmation of pneumonia. Blood samples and broncho-alveolar lavage fluids were obtained from both groups at different time intervals and the peripheral blood mononuclear cells were isolated to evaluate the antimicrobial peptides gene expression. The expression of CD4, Cathelicidin, and Defensins genes was measured in vitro conditions following the addition of 10-6, 10-7 and 10-8 molar of vitamin D3. Defensins and CD4 gene expressions revealed a significant difference in the two groups, at the 48, 72, 96, and 120 hours after the challenge time, while the Cathelicidin gene was not expressed in both experimental groups. Surprisingly, increased expression of Cathelicidin, Defensins, and CD4 was observed at a concentration of 10-6 M of vitamin D3. According to the results of this study, vitamin D3 had positive effects on the immune system which was observed as significant improvement in the clinical symptoms of treated calves. The expression of lung-related Defensins and CD4 in vivo and in vitro approved the immunomodulatory effects of vitamin D3 but the gene expression of Cathelicidin is probably related to prescribing higher doses of vitamin D3. Manuscript profile -
Open Access Article
93 - Some effects of different habitats on antimicrobial activities of (Urtica dioica L.)
elham mogheyseh مه لقا Ghorbanli,Different habitats don’t have the same effects on the growth of plants and different climates can influence the rate of efficient compounds of plants. So, we make a decision for researching about Urtica dioica L. that it’s a medicinal plant and in traditiona MoreDifferent habitats don’t have the same effects on the growth of plants and different climates can influence the rate of efficient compounds of plants. So, we make a decision for researching about Urtica dioica L. that it’s a medicinal plant and in traditional medicine, it has been used for cure of many diseases. During the reproductive period of stinging nettle the sample of this plant was collected from four regions of Gorgan city situated in Golestan province. The ethanol extract of different organs of U. dioica L. was extracted and effected on three bacteries, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans, by using MIC and MBC method. The results of this study showed that ethanol extract has antibacterial and antifungal activity. Prepared extract from high altitude had more antimicrobial effects. It was clear that leaf had most efficient extract among these four evaluated organg (MIC: 62.5mg/ml) and in most cases, stem extract didn’t have antimicrobial effect or its effect was little. But E. coli excluded of this rule and its root extract always was the most efficient extract in all of the altitudes and had the best inhibitory and fatality (MIC 7.8 mg/ml, MBC 15.62 (mg/ml) effects. Manuscript profile -
Open Access Article
94 - Evaluation of phytochemical, cytotoxic, antioxidant and antibacterial activity of of Melissa officinalis L. from Marivan region
Morteza Yazdani fereshteh jookar kashi Zeinab Toluei Akram Rahimi-MoghaddamConsidering the adverse effects of synthetic antioxidants, antibiotics, and anti-cancer drugs on human health and increasing the antibiotic resistance of pathogens, it is a necessity to find safe alternative sources for these compounds. For this purpose, Melissa officin MoreConsidering the adverse effects of synthetic antioxidants, antibiotics, and anti-cancer drugs on human health and increasing the antibiotic resistance of pathogens, it is a necessity to find safe alternative sources for these compounds. For this purpose, Melissa officinalis L. was collected from Marivan, Kurdestan province, and its chemical compositions and bioactivities were determined. Aerial parts of self-sowing M. officinalis were harvested during the flowering stage at 1320 meters above the sea level in 2016 summer. Using solvent-free microwave extraction methodessential oil of M. Officinalis was obtained and their component was identified by gas chromatography/mass spectrometry. The antioxidant and cytotoxicity activity of the essential oil were determined via β–carotene bleaching assay and brine shrimp lethality test, respectively. The antibacterial activity of the essential oil was evaluated by agar well diffusion method and minimum inhibitory concentrationdetermination against various types of standard and clinical microbial strains.Extraction yield of the essential oil was 1.18%, and the main componentswere geraniol (30.39%) and geranial (26.19%).Based on the results of β–carotene bleaching assay, inhibition of linoleic acid oxidation by the essential oil and BHT were 72.48% and 96.48%, respectively.Using the brine shrimp lethality test, LC50 of theessential oil and vincristine sulfate were obtained 62.76 μg/ml and 0.751 μg/ml, respectively.Thus, this study revealed that essential oil of M. officinalis had good antimicrobial activity against testedmicroorganisms, especially Gram-positive bacteria.In addition, it showed significant antioxidant, antibacterial and anticancer activities, so it seems that essential oil of M. officinalis may have potential use as an alternative to synthetic antioxidants, antibiotics and anticancer drugs. Manuscript profile -
Open Access Article
95 - Phytochemical, antioxidant and antibacterial properties in different concentrations of Physalis alkekengi L. extract
simin Arian Seyedeh Fatemeh Habib HosseiniWith the increasing resistance of bacteria to antibiotics and their side effects, the use of medicinal plants in the treatment of bacterial infections is increasing. The aim of this study was to evaluate the phytochemical, antioxidant, and antibacterial properties of di MoreWith the increasing resistance of bacteria to antibiotics and their side effects, the use of medicinal plants in the treatment of bacterial infections is increasing. The aim of this study was to evaluate the phytochemical, antioxidant, and antibacterial properties of different concentrations of methanolic extract of Physalis alkekngi. For this purpose, the calyx and fruit of the P. alkekengi L. were collected from an area near the city of Tonekabon in the summer of 2019 from a height of 120 meters and the antimicrobial activity of the extracts on pathogenic bacteria was determined by disk diffusion methods, MIC, and MBC. Total phenol and flavonoid contents were measured by folin-ciocalteu reagent and aluminum chloride colorimetric method, respectively. In addition, total anthocyanin was measured by spectrophotometry as well as antioxidant activity of extract using DPPH method. Based on the results, the highest total phenol content at a concentration 50 mg-1 ml of calyx extract was 32.92±1.738 mgEGA g-1 DW. Also, the content of flavonoid and anthocyanin compounds in the calyx extract was more than that of the fruit extract. Based on the results of antioxidant activity also showed that the highest DPPH radical inhibitory effect were obtained at concentrations of 100 and 150 mg/ml of calyx extract 51.43% and 51.58%, respectively. In addition, calyx and fruit extracts showed significant inhibitory activity against the studied bacteria as the highest inhibitory effect on Staphylococcus aureus was obtained at a concentration of 1000 mg -1ml of calyx and fruit extracts. The results of this study indicated that the calyx and fruit extracts of P. alkekengi are rich in antioxidant compounds, especially phenolic compounds, and the antimicrobial activity of the extracts showed a significant positive correlation with total phenol content. Therefore, the antimicrobial properties of the calyx and fruit extracts may be attributed to their phenolic compounds and these extracts can be recommended for medicinal applications. Manuscript profile -
Open Access Article
96 - Essential oil composition and antimicrobial activity of arial parts of Lippia citriodora H.B.K from Khuzestan at two phases: before and after blooming
Zahra ZareLippia citriodora H.B.K. is belongs to Verbenaceae family, which has imported to Iran for food and drug consumptions, and today it is cultivating in different parts of the country. In this study the essential oil composition and antimicrobial activity of Lippia citriodo MoreLippia citriodora H.B.K. is belongs to Verbenaceae family, which has imported to Iran for food and drug consumptions, and today it is cultivating in different parts of the country. In this study the essential oil composition and antimicrobial activity of Lippia citriodora methanolic extracts have been studied before and after blooming. The arial parts of the plant before and after blooming were collected from the field in Khuzestan in June 2011. And were dried in laboratory conditions. The Essential oils were isolated by hydro distillation (Clevenger apparatus) and were analyzed by using (GC) and (GC - MS). The methanolic extracts were obtained by percolation method. and the antimicrobial activity of the plant extracts was measured by disk diffusion method and minimum inhibitory concentration against four Gram- positive bacteria, four gram- negative bacteria and two fungi. The main essential oil compounds of arial parts of the plant before blooming were Geraniol (35/78%) and Zingiberene (19/9%) and the main essential oil compounds of arial parts after blooming were Davanone (36/4%) and p-Cymene (16/5%). Both extracts showed significant antimicrobial activity. The antifungal activity of the extracts was more than antibacterial activity. Gram- positive bacteria was more sensitive than Gram- negative bacteria. In addition, while concentrations of the extracts were increasing, the antimicrobial activities were increased in all microorganisms. There was no significant difference between amount of antimicrobial activity of extracts before and after blooming. Manuscript profile -
Open Access Article
97 - Evaluation of phytochemical and antimicrobial activity of the essential oils from different organs of Ferula tabasensis Rech. f. in the natural habitats of Yazd
Razieh MohebatNowadays, considering the side effects of synthetic antibiotics and the increase in drug resistance, it is a necessity to replace them by natural compounds as safe sources. For this purpose, Studies have been carried out on the medicinal properties of essential oils of MoreNowadays, considering the side effects of synthetic antibiotics and the increase in drug resistance, it is a necessity to replace them by natural compounds as safe sources. For this purpose, Studies have been carried out on the medicinal properties of essential oils of different Ferula L. species, but there was no report Ferula tabasensis Rech. f. as an Iranian endemic species. In this research, the leaves and flowers of Ferula tabasensis were harvested from its main habitat (between Kharanaq and Saghand – elevation 1570 m) at the full-flowering stage in the spring of 2021. The essential oils were extracted by hydro distillation method (Clevenger apparatus). GC and GC/MS were used to analyze chemical constituents. The antimicrobial activity of essential oil was evaluated by minimum inhibitory concentration (MIC) determination by broth dilution method, and the growth inhibitory zone diameter (mm) was also measured. The yields of essential oils from the leaves and flowers of the plant were 0.3% and 0.5%, respectively. The results showed that bornyl acetate (34.2-35%) and α-pinene (12.2% and 8.4%) were identified as major compounds of essential oil from leaves and flowers. Also, citronellol (15.5%) is another main constituent found of essential oil from flowers of Ferula tabasensis. Both essential oils had good antimicrobial activity against the tested microorganisms, except E. coli. It seems that antibacterial activity can be mostly attributed to the main compounds of essential oils from the leaves and flowers of the plant, so they may have potential use as alternatives to synthetic antibiotics. Manuscript profile -
Open Access Article
98 - Evaluation of phytochemical, antibacterial, and antifungal properties of Thymus daenensis and Thymus fedtschenkoi from west of Iran
Hasan Mumivand Parisa Khanizadeh Hamid Hassaneian KhoshroIn this study, the essential oils of Thymus daenensis and Thymus fedtschenkoi were investigated in terms of phytochemical, antibacterial, and antifungal properties. After identifying the essential compounds of these two species of thyme, their antimicrobial properties a MoreIn this study, the essential oils of Thymus daenensis and Thymus fedtschenkoi were investigated in terms of phytochemical, antibacterial, and antifungal properties. After identifying the essential compounds of these two species of thyme, their antimicrobial properties against Gram-positive bacteria (Bacillus cereus, Staphylococcus aureus, and Enterococcus faecium), Gram-negative bacteria (Escherichia coli, Salmonella enteritidis), and fungus (Candida albicans) were investigated. For this purpose, the flowering branches of T. daenensis and T. fedtschenkoi were collected at full flowering stage from west of Iran (Arak and Hamedan, respectively) in June. Then, the constituents of the essential oils were identified by gas chromatography-mass spectrometry (GC/MS). To determine the sensitivity of microbial agents to the essential oil of these plants, disk diffusion and microdilution methods were used. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the essential oils of both plants were determined against bacteria and fungi. Based on the results, the essential oil content of T. daenensis (3.4%) was more than that of T. fedtschenkoi (2.9%). The main compositions of the essential oils of T. fedtschenkoi and T. daenensis were linalool (83.1%) and thymol (73.9%), respectively. The essential oils of both species had significant antibacterial and antifungal effects albeit with different levels. In general, the antibacterial activity of T. fedtschenkoi essential oil was higher than that of T. daenensis, especially in the case of gram-positive bacteria. On the other hand, the antibacterial activity of T. daenensis essential oil against E. coli (gram negative) was more than that of T. fedtschenkoi. Antifungal activity of T. daenensis essential oil was higher than that of T. fedtschenkoi against C. albicans. The essential oil of T. fedtschenkoi was rich in linalool, and the higher antimicrobial activity of this species could be attributed to the high percentage of this compound in comparison with T. daenensis. Manuscript profile -
Open Access Article
99 - Evaluation of thermal and antimicrobial behavior of Montmorillonite nanoclay modified with 2-Mercaptobenzothiazole
Milad Edraki Davood Zaarei -
Open Access Article
100 - Preparation of antibacterial coating film using ZnO nanoparticles and epoxy resin
Shakiba Amirsoleimani hamid reza ghorbani -
Open Access Article
101 - Parameters Affecting the Biosynthesis of Gold Nanoparticles Using the Aquatic Extract of Scrophularia striata and their Antibacterial Properties
Yousef Naserzadeh Niloufar Mahmoudi Elena Pakina Imbia Marie Wase Mohamad Heydari Alfred Khodaverdian -
Open Access Article
102 - The preparation of chitosan-Ag nanocomposite for food packaging
Shirin Fahimi Hamid Reza Ghorbani -
Open Access Article
103 - Investigation of Antimicrobial and Genotoxic Effects of Fe2O3, NiO and CoO NPs Synthesized by Green Synthesis
Hayrunnisa Nadaroglu Azize Alaylı Selçuk Çeker Hatice Ogutcu Guleray Agar -
Open Access Article
104 - Biofouling of Reverse Osmosis Membranes in a Petrochemical Complex: Possibility of Anti-Microbial Coating Based on Initial Experimental Results: A Case Study
Kiana AlasvandAbstractIn this root cause analysis, biofouling of membranes in the reverse osmosis (RO) unit of a Petrochemical Complex is studied. The overall cause of getting biofouling was found to be inappropriate water treatment of Pre-RO systems. More specifically, the undertrea MoreAbstractIn this root cause analysis, biofouling of membranes in the reverse osmosis (RO) unit of a Petrochemical Complex is studied. The overall cause of getting biofouling was found to be inappropriate water treatment of Pre-RO systems. More specifically, the undertreatment of water by sand filters, pressurized filters, and cartridges caused increased microbial contamination on RO membranes, leading to biofouling. Biodegradation of polymers is a mechanism by which the act of microorganisms will cause loss of mechanical integrity in such materials. It is important to understand the mechanism and look for ways by which such a mechanism can be controlled. The following were advised to control biofouling in the RO system of this Petrochemical complex 1. Chemical washing of sand filters as pressurized filters as well as RO membranes, 2. Application of UV or removal of chemical chlorination and installation of non-oxidizing biocides after chlorination,3. Replacement of Phosphorus-containing anticalins instead of non-phosphorus sealants,4. Application of superhydrophobic anti-microbial coatings on membranes Manuscript profile -
Open Access Article
105 - Ag/TiO2/Fe3O4@MWCNTs MNCs promoted green synthesis of new spironinhydrine derivatives
Narjes Haerizadeh Anvar Mirzae Atefeh Navabi -
Open Access Article
106 - Ag/TiO2/Fe3O4@MWCNTs MNCs promoted green synthesis of new spironinhydrine derivatives
Morad Avarsaji Zinatossadat Hossaini Ali Varasteh Moradi Hamid Reza Jalilian Ramin Zafar Mehrabian -
Open Access Article
107 - Investigating the possibility of using basil ethanolic extract to control Salmonella Typhimurium in mayonnaise
zahra latifi Sodabeh Moieni Jafar Mohammadzadeh Milany Tara Ghorbani Pir Shahid Parya Rahnama leila Roozbeh NasiraieSalmonella is one of the important food borne diseases, worldwide. Egg and meat are among the most important incriminated foods to cause salmonella infection in Iran. This study was designed to investigate possible inhibitory effects of ethanolic extract of basil MoreSalmonella is one of the important food borne diseases, worldwide. Egg and meat are among the most important incriminated foods to cause salmonella infection in Iran. This study was designed to investigate possible inhibitory effects of ethanolic extract of basil on Salmonella in mayonnaise sauce. Salmonella is killed with baking food but there is problem with foods like sauce which contain raw egg and is not used any procedure for baking them while producing. The purpose of this project was to investigate the possibility to use ethanol extract of basil to control Salmonella Typhimurium in sauce. In the study, first of all the anti-bacterial property of ethanol extract of basil was tested. So, by appearing result test for final investigation densities of extract was purposed 0.25, 0.5 and 0.75%. Then anti-bacterial property of ethanol extract of basil on growth of salmonella in mayonnaise sauce for a period of 30 days in condition of 25oc and 4oc was investigated.The results of statistical studies showed that the three concentrations studied significantly (p <0.05) compared to the control sample reduced the growth of Salmonella Typhimurium during storage and with increasing the concentration of the extract increased the antibacterial activity of the extract so that the extract Basil with a concentration of 7500 ppm was significantly lower than other treatments in most cases (p<0.05). Sensory evaluation test was performed by 5-point hedonic method and the results of sensory analysis of appearance and color in different treatments were not significantly different from each other. Regarding the flavour, texture and total acceptability of the sauce, the lowest sensory score was related to the treatment with a concentration of 0.75%. Based on the obtained results, the amount of bacteria in both temperatures decreased in all treatments and this decrease was little in the control treatment and its antibacterial activity increased with increasing the concentration of the extract. Manuscript profile -
Open Access Article
108 - Study the antimicrobial effects of the essential oils of Origanum vulgare, Mentha piperita and Carum carvi on the bacteria isolates from food stuffs
Mehrdad Ataie KachoueiThe need to replace synthetic preservatives with a natural resource has caused the food manufacturers to use from the medicinal plants as a reservoir. The present study was carried out to evaluate the antimicrobial effects of essential oils extracted from Origanum vulga MoreThe need to replace synthetic preservatives with a natural resource has caused the food manufacturers to use from the medicinal plants as a reservoir. The present study was carried out to evaluate the antimicrobial effects of essential oils extracted from Origanum vulgare, Mentha piperita and Carum carvi on the Escherichia coli, Staphylococcus aureus and Salmonella typhimurium isolated from food products. After preparation of medicinal plants, essence extraction was done using the Clevenger device and antimicrobial effects of essences were evaluated on Escherichia coli, Staphylococcus aureus and Salmonella typhimurium isolated from raw milk and meat. Diameter of inhibition zone each essences was determined using the simple disk diffusion method on Mueller-Hinton agar. Bacterial strains isolates of meat were more resistant to all tested essences but the pattern of the effectiveness of essential oils on isolated strains were similar in both sources. Essential oil extracted from Origanum vulgare had the highest antimicrobial effect on S. aureus and the lowest on E. coli, Mentha piperita had the highest antimicrobial effect on E. coli and the lowest on S. aureus and Carum carvi had the highest antimicrobial effect on S. aureus and the lowest on S. typhimurium. According to the antibacterial effects of the mentioned essential oils, the possibility of their application as an antimicrobial preservatives in meat and its products and also dairy products dairy is exist. Manuscript profile -
Open Access Article
109 - Inhibitory effect of Basil (Ocimum basilicum L.) essence on Staphylococcus aureus, Escherichia coli and Rhodotorula rubra
Fareba Bakhshi hamid mirzaii نارملا آصفیApplication of natural preservatives to protect foods from microbial spoilage and to control the occurrence of foodborne pathogens has become an important issue worldwide. The aim of this study was to investigate the inhibitory effect of Basil (Ocimum basilicum L.) esse MoreApplication of natural preservatives to protect foods from microbial spoilage and to control the occurrence of foodborne pathogens has become an important issue worldwide. The aim of this study was to investigate the inhibitory effect of Basil (Ocimum basilicum L.) essence of Staphylococcus aureus, Escherichia coli and Rhodotorula rubra. The basil essence was extracted by hydro-distillation using Clevenger apparatus. The components of the essence was analyzed by GC/MS. Afterwards, using micro-dilution method the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) for S. aureus, E. coli and R. rubra was estimated. Based on results, 43 various components were detected in basil essence, namely benzene (20.97%), E-citral (12.845%), Z-citral (6.137%), methyl chavicol (7.68%) and thymol (7.79%). MIC and MBC for was 0.25 mg/ml for E. coli and R. rubra, meanwhile it was estimated at 0.5 mg/ml for S. aureus. Taking into account the various components of basil essence as well as their antimicrobial properties, it was concluded that, with considering the natural properties of each food type, basil essence can be used as a food preservative.a Manuscript profile -
Open Access Article
110 - Chemical composition and antibacterial activity of the essential oil from Shirazi Turnip root (Brasica rappa L.) in in-vitro conditions
Ali Mohamadi Sani Khalil Behnam Mehrnaz EsmaeilpourThe aim of this study was to evaluate the chemical composition and antimicrobial activity of essential oil of Turnip (Brassica rapa) root in Fars-Iran. The essential oil was obtained by hydro-distillation and analyzed by gas chromatography-mass spectrometry (GC-MS) whic MoreThe aim of this study was to evaluate the chemical composition and antimicrobial activity of essential oil of Turnip (Brassica rapa) root in Fars-Iran. The essential oil was obtained by hydro-distillation and analyzed by gas chromatography-mass spectrometry (GC-MS) which revealed 16 compounds in which Methyl chavicol (32.31 %), Trans anethole (19.58 %), Linalool (15.46 %), α-Pinene (9.28 %), α- Thujone (6.59 %) and β- Pinene (3.34 %) were the main components. The antimicrobial activity was measured by disk-diffusion and micro-dilution method for determination of MIC and MBC. The results showed that the essential oil of turnip has a significant effect in reducing the growth of bacteria have been tested. However the essential oil had the lowest MIC on (MIC=2.5 mg/ml) E. coli but the results showed the highest effects against (MIC=0.625 mg/ml) S. aureus. Results presented here suggest that the essential oils of turnip possesses antibacterial properties, and is therefore a potential source of active ingredients for food industry. Manuscript profile -
Open Access Article
111 - Prevalence and antimicrobial resistance of Listeria species isolated from filleted Argyrosomus hololepidotus, Scomberomorus commerson and Alburnus spp.
Ebrahim Rahimi Mohammad Javad Jahanmard Sohrab safari Mahsa Ansari Zeinab Torki baghbadoraniListeriosis is one of the most important food-borne diseases caused by Listeria species, especially L. monocytogenes. The objective of this study was to determine the prevalence and antimicrobial resistance of Listeria species isolated from three types of fish fil MoreListeriosis is one of the most important food-borne diseases caused by Listeria species, especially L. monocytogenes. The objective of this study was to determine the prevalence and antimicrobial resistance of Listeria species isolated from three types of fish filet in Isfahan and Bandaranzali. From August 2009 to April 2011, a total of 120 samples of Argyrosomus hololepidotus (n= 90), Scomberomorus commerson (n=80) and Alburnus spp (n=70). Fish were obtained from randomly selected retail stores in Isfahan and Bandaranzali cities and were evaluated for the presence of Listeria spp. using standard cultural and PCR methods. Then antibiogram tests were carried out for determination of antimicrobial resistance. Seven (8.8%) and 6 (15%) of smoked and salted fish samples were positive for Listeria spp. respectively. L. monocytogenes, L. innocua and L. seeligari were isolated from 2.5, 6.7 and 1.6% of fish samples. Overall, 9 of 13 Listeria isolates (69.2%) were resistant to one or more antimicrobial agents. Resistance to tetracycline (53.8%) and tetracycline (30.8%) were the most common finding. All of the isolates were susceptible to gentamicin, vancomycin and chloramphenicol. The results of this study indicate the potential risk of infection with Listeria in people consuming raw or undercooked smoked and salted fish. Also, the results obtained in this study indicated the need for appropriate surveillance and epidemiological monitoring strategy to control the development of resistance. Manuscript profile -
Open Access Article
112 - Antibacterial activity of acetonic, ethanolic and methanolic olive leaf extracts of four Iranian cultivars against Escherichia coli
Maryam Abbasvali Mahmoud Esmaeili Koutamehr Hamdollah Moshtaghi Mohammad EskandariThe aim of this study was to evaluate the antibacterial activity of olive leaf extracts from four important Iranian native cultivars (Dezfol, Roghani, Zard and Shiraz) against E. coli. Acetonic, methanolic and ethanolic olive leaf extracts were prepared using a microwav MoreThe aim of this study was to evaluate the antibacterial activity of olive leaf extracts from four important Iranian native cultivars (Dezfol, Roghani, Zard and Shiraz) against E. coli. Acetonic, methanolic and ethanolic olive leaf extracts were prepared using a microwave. The extracts were concentrated and lyophilized. The growth inhibition effect of different concentrations of extracts was determined using the broth micro-dilution assay. The growth curves of E. coli during 24 h incubation at 37°C were drawn. The area under the bacterial growth curve and the percentage inhibition of the growth were calculated. Extracts with the concentrations of 5 and 10 mg/ml had no considerable inhibition effect on the bacterium. Acetonic extract of Dezfol and Zard cultivars with the concentration of 20 mg/ml inhibited the bacterial growth 93% and 94.9%, respectively and showed significantly stronger inhibition effect in comparison with acetonic extract of Shiraz and Roghani cultivars. Methanolic extract of Roghani cultivar with the bacterial growth inhibition of 93.4% showed significantly stronger effect in comparison with its acethonic and ethanolic extracts. Ethanolic extracts of four studied cultivars showed no antibacterial effect. Among the four important Iranian native olive leaf cultivars, Dezfol and Zard were found to possess the highest antimicrobial activity and aceton was the best solvent. On the basis of these results, it can be concluded that extracts of the Iranian native olive leaf cultivars could be used as a natural antimicrobial agent in food preservative, ointment and drugs. Manuscript profile -
Open Access Article
113 - Molecular identification and evaluation of antimicrobial effects of dominant LAB isolated from Tarkhineh and its bacteriocin-like substances on some foodborne microorganisms
Atena Sarani علیرضا صادقی Morteza Khomeiri Yahya Maghsoudlou Ali Moayedi Maryam EbrahimiIsolation and evaluation of the characteristics of lactic acid bacteria (LAB) from traditional fermented products have always the possibility of exposure to isolates with unique capabilities.In the present study after molecular identification of dominant LAB isolated fr MoreIsolation and evaluation of the characteristics of lactic acid bacteria (LAB) from traditional fermented products have always the possibility of exposure to isolates with unique capabilities.In the present study after molecular identification of dominant LAB isolated from Tarkhineh, antibacterial and antifungal effects of the isolate and its bacteriocin-like substances on some foodborne indicators were investigated in accordance with the reference methods. Sequencing results of polymerase chain reaction led to the identification of Lactococcus lactis as dominant isolate. LAB isolate had proper antibacterial effect but there was no significant difference (P<0.05) between growth inhibitory diameter of S. aureus and E. coli in present of the isolate. Furthermore, the inhibitory effect of the crude supernatant was higher than that of the neutralized and treated supernatants against studied indicator bacteria. The highest reduction in the population of S. aureus (87.91%) was also observed in the presence of crude supernatant obtained from Logarithmic growth phase. The LAB isolate had the higher antifungal effect on A. flavus rather than A. niger, but the growth rate of A. niger compared to A. flavus was remarkably (P<0.05) higher in each of the third to sixth days of incubation in the presence of bacteriocin-like substances. Based on the results of this study, Lactococcus lactis isolate and its bacteriocin-like substrates can be used as starter culture in fermented food processing, or as bio-preservative in food and pharmaceutical industries. Manuscript profile -
Open Access Article
114 - The Effect of Thyme (Zataria multiflora boiss) Essential Oil against Bacterial and Fungal strains in Mayonnaise
Bahareh Hajimohammadi- Telkabadi Asiye Ahmadi-DastgerdiEssential oils are considered as alternative to synthetic preservatives in food products because of their antimicrobial properties. The aim of this study was to identify the main components of thyme (Zataria multiflora boiss) essential oil and to evaluate the antimicrob MoreEssential oils are considered as alternative to synthetic preservatives in food products because of their antimicrobial properties. The aim of this study was to identify the main components of thyme (Zataria multiflora boiss) essential oil and to evaluate the antimicrobial activity of essential oil in vitro and mayonnaise. The essential oil was extracted by distillation and analyzed by gas chromatography. The antimicrobial activity of the essential oil was evaluated by determining the minimum inhibitory concentration and minimum bactericidal concentration. GC analysis of the essential oil resulted in the identification of forty compounds by a high number of monoterpenes such as thymol and carvacrol. The most susceptible pathogens were the fungi (lowest MIC) and the most resistant pathogen to Gram-negative bacteria. This study confirms that the essential oil of thyme possessed antimicrobial properties in vitro. In the second phase, efficiency of essential oil as natural preservative in high fat and low fat mayonnaise kept during storage at 4 oC for 6 months was studied. The results showed that of essential oil had influence against all of the tested microorganisms in mayonnaise and the pathogens did not grow in mayonnaise formulations, whereas in the control mayonnaise all of the pathogens and fungus grew. In conclusion the essential oil of thyme (Zataria multiflora boiss) would lead to control food pathogen and food spoilage organisms as natural food preservative and therefore, it would be used in biotechnological fields as natural preservative ingredients in food industry. Manuscript profile -
Open Access Article
115 - Antimicrobial Effect of Biosynthesized Silver Nanoparticles Using Rosemary (Rosmarinus officinalis) Extract on Some Soft Rot Bacteria in Storage Condition
Esmaiil Torkabadi Zeinab Fotoohiyan fatemeh shahdadiNowadays, use of plants in the synthesis of metal nanoparticles, which have antimicrobial activity, is considered. The aim of present study was evaluating biosynthesis of silver nanoparticles from aqueous extract of rosemary and then investigating the effect of these na MoreNowadays, use of plants in the synthesis of metal nanoparticles, which have antimicrobial activity, is considered. The aim of present study was evaluating biosynthesis of silver nanoparticles from aqueous extract of rosemary and then investigating the effect of these nanoparticles and mancoseb toxin on soft rot bacteria isolated from carrots, potatoes and onions during storage. The biosynthesized silver nanoparticles were analyzed by ultraviolet and FTIR spectroscopy. Soft rot bacteria were isolated and identified using selective and specific culture media from carrots, potatoes and onions. A pathogenic isolate from each sample with the highest pathogenicity was selected for the tests and their minimum inhibitory concentration (MIC) was evaluated against the experimental treatments. The MIC of the pathogen isolates against the 2580 mg/ml of silver nanoparticles and 1000 ppm of mancoseb were 88.88% and 100%, respectively. The mean percentage of vegetable contamination with pathogen isolate in the presence of silver nanoparticles in the third month of storage was 70%, 80% and 70% for onions, potatoes and carrots, respectively, and average percentage of vegetable contamination with pathogenic isolates in the combined control of bacteria with silver nanoparticles and mancoseb toxin at the third month of storage for onion, potato and carrot were 45, 40 and 40%, respectively. According to obtained results, the combined control of biosynthesized silver nanoparticles and Mancoseb can be used to control of soft rot bacteria in carrot, potato and onion. Manuscript profile -
Open Access Article
116 - Comparative Study of Antibacterial Effects of Polar, Semi-polar and non-polar Fractions of Lnula helenium Plant Extract against some food-borne bacteria
Hosein Soleimani behboud jafariToday, chemical drugs are replaced by herbal medicines because of the harmful effects they place on the human body. Although the proportion of herbal medicines to chemical drugs in the world is negligible, the replacement process is followed more quickly. The aim of thi MoreToday, chemical drugs are replaced by herbal medicines because of the harmful effects they place on the human body. Although the proportion of herbal medicines to chemical drugs in the world is negligible, the replacement process is followed more quickly. The aim of this study was to investigate the antibacterial effect of polar, semi-polar and non-polar fractions of Lnula helenium extract on some food-borne bacteria . In this study, Lnula helenium plant was collected from Arak natural areas and then extracts of methanolic, chloroform and hexane of Lnula helenium were obtained. The effect of different concentrations of this extract was investigated. All experiments were carried out using Agar well diffusion and determination of MIC and MBC on standard strains of Pseudomonas aeruginosa, Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Streptococcus mutans. Methanol extract of Lnula helenium(6.25 μg / ml) inhibits the growth of Gram-positive and Gram-negative bacteria. Chloroform extract with minimum concentrations (12.5 mg / ml, 50 and 100%) had lethal effect on Streptococcus mutans, Staphylococcus aureus and Listeria monocytogenes, respectively and hexane extract only affected gram-positive bacteria. The herbal extract can be used as an herbal medicine after completing clinical trials. Keywords: Antimicrobial Effects, Extract, Lnula helenium Manuscript profile -
Open Access Article
117 - Antimicrobial activity of aqueous, alcoholic and buffer extracts of honey-bee propolis on oral-intestinal bacteria
Mahnoush Aboutorab Mohammad Goli Elham KhosraviPropolis plays an antibacterial, anti-fungal, anti-parasitic, antioxidant, anti-inflammatory, anti-caries role due to its plant-derived compounds such as polyphenols and proteins such as 10-HDA. It has long been a therapeutic role for diseases, especially infections. In MorePropolis plays an antibacterial, anti-fungal, anti-parasitic, antioxidant, anti-inflammatory, anti-caries role due to its plant-derived compounds such as polyphenols and proteins such as 10-HDA. It has long been a therapeutic role for diseases, especially infections. In this study antimicrobial activity of alcoholic, aqueous and buffer extract of propolis, on oral bacteria including Streptococcus mutans, Streptococcus epidermidis, Staphylococcus aureus, and gastro-intestinal bacteria including Streptococcus salivarius and Escherichia coli measured using micro broth dilution test for assessing of minimum inhibitory concentration (MIC) and disk diffusion test for assessing of diameter of inhibition zone. The MIC did not differ significantly between the three alcoholic, aqueous and buffer extracts, i.e., the effect of the solvents on the solubility of the antibacterial compounds was approximately the same (P > 0.05). The most resistant gram-positive bacterium to alcoholic and buffer extract, Streptococcus mutans and the most resistant to all extracts, Escherichia coli, were reported. There was no significant difference between the bacterial resistance in the aqueous extract of propolis (P> 0.05). In the disk diffusion test, increasing the concentration of extracts had a significant effect on the bacterial killing rate and was identified as the most susceptible bacterium to the extracts Staphylococcus epidermidis and the most resistant bacterium Staphylococcus aureus. The bacteria were also more sensitive to buffer extract. Manuscript profile -
Open Access Article
118 - Antimicrobial and Antioxidant Effects of Ethanolic Extract of Rosa canina Fruit on Increasing the Shelf Life of Chicken Meat Kept at Rrefrigerator Temperature
Sedigheh Yazdanpanah Fatemeh ShirvaniRosa canina fruit extract with its high content of bioactive compounds such as vitamin C, carotenoids and polyphenolic compounds, can be used as a natural preservative in meat products. The aim of this study was to investigate the antimicrobial and antioxidant activity MoreRosa canina fruit extract with its high content of bioactive compounds such as vitamin C, carotenoids and polyphenolic compounds, can be used as a natural preservative in meat products. The aim of this study was to investigate the antimicrobial and antioxidant activity of Rosa canina fruit extract to improve durability at refrigerator temperature. For this purpose, ethanolic extract of Rosa canina fruit was prepared by microwave at three concentration levels (0.5%, 1% and 2%) and added to chicken samples. An extract-free sample was considered as a control. All specimens were kept at 4 °C for 10 days. Microbial properties including: Total bacterial count, Escherichia coli, Staphylococcus coagulase positive and Salmonella, physicochemical properties including: fat oxidation, free radical inhibition, methemoglobin absorption, pH and volatile nitrogen during the first, fourth, seventh and tenth days were tested. The results showed that Rosa canina Fruit extract significantly reduced the mean (P Manuscript profile -
Open Access Article
119 - Study the antimicrobial effects of ethanolic extract of Thymus vulgaris on foodborne bacteria in vitro
Meysam Alimoradi Mehrdad Ataie Kachoie Fariborz MoattarThymus vulgaris is a plant of family Lamiaceae that has high antimicrobial properties. The present study was performed to evaluate the antimicrobial effects of the ethanolic extract of Thymus vulgaris on food-borne bacteria. Aerial part of Thymus vulgaris was used to pr MoreThymus vulgaris is a plant of family Lamiaceae that has high antimicrobial properties. The present study was performed to evaluate the antimicrobial effects of the ethanolic extract of Thymus vulgaris on food-borne bacteria. Aerial part of Thymus vulgaris was used to prepare methanolic extract. The diameter of the growth inhibition zone of bacteria was assessed using disk diffusion. Minimum inhibitory concentration (MIC) and Minimum Bacterial Concentration (MBC) of ethanolic extract of Thymus vulgaris were evaluated on the target bacteria using ELISA plate. The diameter of the growth inhibition zone of bacteria against the ethanolic extract of Thymus vulgaris ranged from 7.87±0.61 to 16.99±0.37 mm. Application of 50 mg/ml concentration of ethanolic extract of Thymus vulgaris caused the highest diameter of the growth inhibition zone of Pseudomonas aeruginosa (11.38±0.43 mm), Escherichia coli (12.00±0.35 mm), Staphylococcus aureus (16.33±0.69 mm) and Listeria monocytogenes (16.99±0.37 mm). The antimicrobial effects of ethanolic extract of Thymus vulgaris were dose-dependent (P <0.05). The diameter of growth inhibition zone of 50 mg/ml of ethanolic extract of Thymus vulgaris on Pseudomonas aeruginosa, Staphylococcus aureus and Listeria monocytogenes was higher than all antibiotics (P <0.05). The lowest MIC and MBC values were obtained for Staphylococcus aureus (4 and 6 mg/ml, respectively) and Listeria monocytogenes (4 and 6 mg/ml, respectively). Thymus vulgaris ethanolic extract can be used as a combination with antimicrobial properties in food. Manuscript profile -
Open Access Article
120 - Study the antimicrobial effects of methanolic extract of Hypericum perforatum on foodborne bacteria
Leila Ghodrati Mehrdad Ataie Kachoie Sadegh Mousavi-Fard Fariborz MoattarHypericum perforatum is a medicinal plant of the genus Hypercasia that has phenolic and flavonoid compounds with high antimicrobial and antioxidant properties. The present study was performed to evaluate the antimicrobial effects of the methanolic extract of Hypericum p MoreHypericum perforatum is a medicinal plant of the genus Hypercasia that has phenolic and flavonoid compounds with high antimicrobial and antioxidant properties. The present study was performed to evaluate the antimicrobial effects of the methanolic extract of Hypericum perforatum on food-borne bacteria. Aerial part of Hypericum perforatum was prepared from the research farm, and after approval by experts, it was dried and powdered and used to prepare methanolic extract. The diameter of the growth inhibition zone of Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus was assessed using disk diffusion and compared with antibiotics. Minimum inhibitory concentration (MIC) and Minimum Bacterial Concentration (MBC) of methanolic extract of Hypericum perforatum were evaluated on the target bacteria using ELISA plate. The diameter of the growth inhibition zone of bacteria against the methanolic extract of Hypericum perforatum ranged from 9.33±0.45 to 15.28±0.60 mm. Application of 100 mg/ml concentration of methanolic extract of Hypericum perforatum caused the highest diameter of the growth inhibition zone of Pseudomonas aeruginosa (12.27±0.53 mm), Escherichia coli (13.20±0.59 mm) and Staphylococcus aureus (15.28±0.60 mm). The antimicrobial effects of methanolic extract of fenugreek were dose-dependent (P <0.05). The lowest and highest levels of MIC and MBC of methanolic extract of Hypericum perforatum were obtained for Staphylococcus aureus (0.0010 and 0.0019, respectively) and Escherichia coli (0.50 and 1.00, respectively). The diameter of the growth inhibition zone of bacteria treated with methanolic extract of Hypericum perforatum was higher compared to some antibiotic discs. Manuscript profile -
Open Access Article
121 - Introducing an optimal mixture of Origanum majorana, Long pepper, and Areca extracts with the highest level of activity against gene expression of A and B Staphylococcus aureus enterotoxins.
Seyyedjavad Naddafi Ali Mohammadi Sani Esmaeil Ataye Salehi Reza KarazhyanWith the growing awareness of the importance of natural extracts with antimicrobial and antioxidant properties, today the food industry is looking for the use these compounds instead of synthetic preservatives. To date, no research has been done to find an optimal mixtu MoreWith the growing awareness of the importance of natural extracts with antimicrobial and antioxidant properties, today the food industry is looking for the use these compounds instead of synthetic preservatives. To date, no research has been done to find an optimal mixture of Origanum majorana, Long pepper, and Areca extracts against the gene expression of A and B enterotoxins of S. aureus. Therefore, in this study, we are seeking to provide a mixture of these extracts with the highest anti-enterotoxigenic activity using real-time PCR and mixture statistical methods. If successful, we introduce these extracts as an antagonist against the growth of pathogenic bacteria and the gene expression of enterotoxin of S. aureus. In the first stage, the antimicrobial activity of Origanum majorana, Long pepper, and Areca extracts was studied, which Origanum majorana had the highest antimicrobial activity among them. On the other hand, among the tested bacteria, gram-positive bacteria were more sensitive than gram-negative bacteria. The tested extracts had a synergistic effect on each other against the gene expression of A and B enterotoxins of S. aureus. The mixture of extracts including 47% Origanum majorana extract, 27% Long pepper extract, and 26% Areca extract showed the highest activity against the gene expression. Manuscript profile -
Open Access Article
122 - Evaluation of contamination of raw milk and traditional butter in Amol city with Listeria monocytogenes and determination of their antibiotic resistance.
Negin Najafi Mahdi Sharifi Soltani Atefeh Bozorgi MakeraniListeria is one of the most important bacteria that contaminates milk and milk products. The main species that causes listeriosis in humans and animals and is transmitted through contaminated food is Listeria monocytogenes. For this purpose, in order to identify Listeri MoreListeria is one of the most important bacteria that contaminates milk and milk products. The main species that causes listeriosis in humans and animals and is transmitted through contaminated food is Listeria monocytogenes. For this purpose, in order to identify Listeria monocytogenes in raw milk and traditional butter, in the spring of 1400, 40 samples of raw milk (20) and traditional butter (20) were collected from traditional dairy stores in Amol city. The samples were immediately transferred to a food microbiology laboratory in cold condition. The samples were first cultured in peptone water enrichment medium and then the enriched samples were transferred to Palkam Listeria selective agar medium with selective Listeria monocytogenes supplementation. Then antimicrobial susceptibility of Listeria monocytogenes isolates was determined by disk diffusion method using the antibiotics ampicillin, gentamicin, erythromycin, chloramphenicol, tetracycline, and ciprofloxacin. From the total samples, the prevalence of Listeria species in raw milk and butter was 35% and 10%, respectively. The results of antibiotic susceptibility and resistance test of raw milk samples showed that they were resistant to ampicillin 85.7% and tetracycline 71.4% and among butter samples only erythromycin was 100% resistant and ampicillin 100% semi-resistant. The mean of the obtained results in the antibiogram test were analyzed with 95% confidence and one-way analysis of variance. The results of the present study indicate the potential risk of infection due to consumption of raw milk and traditional butter to Listeria monocytogenes and raw milk had higher risk than butter. Manuscript profile -
Open Access Article
123 - Evaluation of the antimicrobial activity of Melissa officinalis essential oil on foodborne bacteria
Salman Heidarian Mehrdad Ataie Kachoie Sadegh Mousavi-Fard Fariborz MoattarMelissa officinalis is a medicinal plant with high antimicrobial properties. The present study was performed to evaluate the antimicrobial effects of Melissa officinalis essential oil on food-borne bacteria. Melissa officinalis aerial parts were prepared and after appro MoreMelissa officinalis is a medicinal plant with high antimicrobial properties. The present study was performed to evaluate the antimicrobial effects of Melissa officinalis essential oil on food-borne bacteria. Melissa officinalis aerial parts were prepared and after approval by experts used to prepare essential oils. The diameter of the growth inhibition zone of Listeria monocytogenes, Salmonella typhimurium, Escherichia coli and Staphylococcus aureus was assessed using disk diffusion and compared with antibiotics. The minimum inhibitory concentration (MIC) and the minimum bacterial concentration (MBC) of Melissa officinalis essential oil were evaluated on the target bacteria. Citronellal (14.4%) and Caryophyllene oxide (11%) were the most important compounds identified in Melissa officinalis essential oil. The diameter of the growth inhibition zone of the bacteria against Melissa officinalis essential oil ranged from 3.93±0.42 to 15.23±1.17 mm. Application of 4 mg/ml concentration of Melissa officinalis essential oil caused the largest diameter of the growth inhibition zone of Staphylococcus aureus (14.70±0.27 mm), Escherichia coli (10.33±0.29 mm) and Salmonella typhimurium (10.18±0.52 mm). The antimicrobial effects of Melissa officinalis essential oil were dose-dependent (P <0.05). The lowest and highest levels of MIC and MBC of Melissa officinalis essential oil were obtained for Staphylococcus aureus (1.25 and 2.5, respectively) and Salmonella typhimurium (10 and 20, respectively). The diameter of the growth inhibition zone of bacteria treated with Melissa officinalis essential oil was higher than that of many antibiotics, but lower than that of vancomycin. Melissa officinalis essential oil can be used as a substance with antimicrobial properties, especially in food films. Manuscript profile -
Open Access Article
124 - A review on the use of Essential oils as natural antimicrobial agents in meat and meat products
Seyedeh Khadijeh Ahmadisheikhshabani seyedeh fatemeh ahmadi Azizollah Fallah MehrjardiWith the increase in the amount of food production, the need for maintenance is felt in order to deliver healthy and high-quality food to consumers. Today, extensive research has been done in relation to the use of natural antimicrobial compounds to deal with pathogens MoreWith the increase in the amount of food production, the need for maintenance is felt in order to deliver healthy and high-quality food to consumers. Today, extensive research has been done in relation to the use of natural antimicrobial compounds to deal with pathogens of food origin, and it has been determined that these compounds are a suitable alternative to chemical antimicrobial compounds. Plant essential oils have good antimicrobial properties due to the presence of polyphenolic compounds. Meat is the main source of protein of animal origin, which is considered one of the most perishable foods due to its abundant nutrients. Nowadays, the use of food coatings containing natural antimicrobial and antioxidant agents, including plant essential oils, is one of the new methods of preservation, which has a good potential to prevent pathogenic microorganisms and also increase the shelf life. They have the shelf life of meat products and are able to increase the shelf life and preserve the natural appearance of meat and meat products. It is interesting to note that active films containing essential oils provide an environmentally friendly solution in the meat industry. In general, active food packaging has shown a suitable technology for improving meat quality and reducing waste in the food industry. This study was conducted with the aim of investigating the antimicrobial effects of different plant essential oils and their synergistic effects in meat preservation. Manuscript profile -
Open Access Article
125 - اثر ضدباکتریایی عصاره های اندام هوایی و ریشه چای کوهی (Stachys lavandulifolia Vahl) در دو مرحله رشد
مسعود طاهری سامان مهدوی یوسف مهمان نوازمقدمه و هدف: امروزه جایگزینی داروهای طبیعی و مورد استفاده در طب سنتی به جای داروهای شیمیایی و سنتزی مورد توجه اغلب پژوهشگران قرار گرفته است. هدف از تحقیق حاضر مطالعه اثرات ضدمیکروبی عصاره های آبی، الکلی و متانولی اندام هوایی و ریشه گیاه گل سنبله ای با نام علمی Stachys l Moreمقدمه و هدف: امروزه جایگزینی داروهای طبیعی و مورد استفاده در طب سنتی به جای داروهای شیمیایی و سنتزی مورد توجه اغلب پژوهشگران قرار گرفته است. هدف از تحقیق حاضر مطالعه اثرات ضدمیکروبی عصاره های آبی، الکلی و متانولی اندام هوایی و ریشه گیاه گل سنبله ای با نام علمی Stachys lavandulifolia Vahl در دو مرحله رویشی جمع آوری شده از مرند استان آذربایجان شرقی بود.مواد و روش: فعالیت ضدمیکروبی عصاره های مختلف اندام هوایی و ریشه گیاه گل سنبله ای با نام علمی Stachys lavandulifolia Vahl جمع آوری شده در دو مرحله رویشی علیه Staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 8739), Pseudomonas aeruginosa (ATCC 9027) به روش رقت سازی مورد مطالعه قرار گرفت:نتایج و بحث: نتایج خاصیت ضد میکروبی عصاره گل سنبله ای نشان داد که عصاره آبی گیاه مذکور فاقد اثر ضدمیکروبی در حالی که عصاره های الکلی 80 درصد متانول و اتانول دارای خاصیت ضدمیکروبی موثری بودند. خصوص این اثر ضدباکتریایی بر باکتری های گرم مثبت بیشتر بود. همچنین خاصیت ضدباکتریایی گل آذین گیاه گل سنبله ای بیشتر از اندام ریشه بود.توصیه صنعتی /کابربردی: باتوجه به نتایج حاصل، عصاره الکلی سرشاخه های گل سنبله ای دارای خاصیت ضدبکتریایی قابل ملاحظه ای بود که می توان از آن به عنوان یک ضدمیکروب طبیعی استفاده نمود. Manuscript profile -
Open Access Article
126 - بررسی خاصیت ضد میکروبی عصاره تام و سه فراکشن Platychaeteaucheri (Boiss.) Boiss علیه 5 سویه استاندارد میکروبی و سویه های بالینی اشریشیاکلی
تینا ذبیحی نیک مژده حاکمی-والا فاطمه باقری بجستانیمقدمه و هدف:Platychaeteaucheri (Boiss.) Boiss متعلق به خانوادهAsteraceae بوده و یکی از گیاهان بومی ایران است. هدف از این مطالعه، بررسی خاصیت ضد میکروبی عصاره تام متانولی و سه فراکسیون حاصل از بخش هوایی گلدار Platychaete aicheri بر روی تعدادی باکتری گرم مثبت Moreمقدمه و هدف:Platychaeteaucheri (Boiss.) Boiss متعلق به خانوادهAsteraceae بوده و یکی از گیاهان بومی ایران است. هدف از این مطالعه، بررسی خاصیت ضد میکروبی عصاره تام متانولی و سه فراکسیون حاصل از بخش هوایی گلدار Platychaete aicheri بر روی تعدادی باکتری گرم مثبت و گرم منفی استاندارد، مخمر کاندیدا آلبیکنس و همچنین نمونههای بالینی اشریشیاکلی جدا شده از عفونت ادراری و زخم سوختگی است. روش تحقیق: عصاره تام متانولی Platychaeteaucheri با روش خیساندن تهیه شد. سپس فراکسیونهای کلروفرمی، اتردوپترولی و آبی با استفاده از روش استخراج مایع-مایع بدست آمدند. اثر ضدمیکروبی با روشهای انتشار در چاهک و براث میکرودایلوشن و بر اساس پروتکل CLSI مورد بررسی قرار گرفت. سویههای استاندارد مورد بررسی شامل: اشریشیاکلی (1399 PTCC)، سودوموناس آﺋروژینوزا (1430 PTCC)، استافیلوکوکوس ارﺋوس (1431 PTCC)، باسیلوس سرﺋوس (1247 PTCC) و کاندیدا آلبیکنس (5027 PTCC) بودند. همچنین عصاره و فراکسیونها بر روی سویههای اشریشیاکلی بالینی که از نمونه ادرار بیماران بیمارستان امام خمینی تهران که در سال 2013 و زخمهای سوختگی بیماران بیمارستان شهید مطهری تهران که در سال 2014 جدا شده بودند نیز مورد بررسی قرار گرفتند. نتایج و بحث: عصاره تام و فراکسیونهای کلروفرمی و اتردوپترولی دارای خاصیت ضدمیکروبی متوسط بر روی سودوموناس آﺋروژینوزا و اشریشیاکلی استاندارد با محدوده MIC (mg/ml 42-35) بودند. دامنه MIC برای ایزولههای بالینی اشریشیاکلی در محدوده mg/ml 72-60 بود. فراکسیون آبی فعالیت ضدمیکروبی کمتری نسبت به عصاره تام و سایر فراکسیونها بر روی نمونههای استاندارد و بالینی داشت. بر اساس یافتهها فراکسیونهای اتردوپترولی و کلروفرمی اثر آنتیباکتریال بیشتری نسبت به عصاره تام متانولی و فراکسیون آبی داشتند. پیشنهادهای کاربردی و صنعتی: بر اساس نتایج در صورت عدم سمیّت در مطالعات آتی، این گیاه میتواند به عنوان یک منبع آنتیباکتریال طبیعی در محصولاتی جهت کمک به درمان عفونتهای مجرای ادراری و سوختگی مورد استفاده قرار گیرد. Manuscript profile -
Open Access Article
127 - مطالعه مقایسه ای ترکیبات شیمیایی و خواص ضد باکتریایی روعن های اسانسی مینای ترکمنستانی( Tanacetum turcomanicum (Krash و مینای کپت داغی(Tanacetum walteri (C.Winkl) از استان خراسان شمالی
علی فیروزنیا فهیمه دوست زاده نرگس ربیعی زمینه و هدف: جنس مینا، از تیره­ کاسنی می­ باشد که تعداد 12 گونه از آن مانند مینای کپت داغی انحصاری ایران است. در این تحقیق، ترکیبات شیمیایی و فعالیت ضد­میکروبی اسانس­های مینای ترکمنستانی و مینای کپت داغی مورد مطالعه قرار گرفت. روش تحقیق:ان More زمینه و هدف: جنس مینا، از تیره­ کاسنی می­ باشد که تعداد 12 گونه از آن مانند مینای کپت داغی انحصاری ایران است. در این تحقیق، ترکیبات شیمیایی و فعالیت ضد­میکروبی اسانس­های مینای ترکمنستانی و مینای کپت داغی مورد مطالعه قرار گرفت. روش تحقیق:اندام­های هوایی گیاهان مورد مطالعه در بهار 1395 از استان خراسان شمالی، ایران جمع آوری شد. اسانس­ها به روش تقطیر با آب و با استفاده از دستگاه کلونجر استخراج شدند. شناسایی ترکیبات شیمیایی اسانس توسط دستگاه GC/MS انجام شد. همچنین برای بررسی اثرات ضد میکروبی اسانس بر روی باکتری­های اشرشیا کلی، استافیلوکوکوس اورئوس، باسیلوس آتروفئوس و میکروکوکوس لوتئوس از روش انتشار در آگار استفاده شد. نتایج: راندمان اسانس گونه­ مینای کپت داغی 2/0درصد حجمی/وزنی به دست آمد. در مجموع 84/92 درصد از کل ترکیبات اسانس شامل 43 ترکیب شناسایی گردید. آلفا- پینن (37/22 درصد)، 1و8-سینئول ( 52/12 درصد)، کامفور ( 91/11 درصد) و بتا- توجون (52/4 درصد) به عنوان ترکیبات اصلی شناسایی شدند. از تجزیه­ی اسانس گونه­ی مینای ترکمنستانی،39 ترکیب و 28/89 درصد از کل ترکیبات اسانس با راندمان 3/0 درصد حجمی/ وزنی شناسایی شدند. داوانون (79/20 درصد)، 1و8-سینئول (63/15 درصد)، پاراسیمن (20/10 درصد)، کامفور (14/10 درصد) و بورنئول (95/6 درصد) ترکیبات اصلی اسانس را تشکیل می دادند. بالاترین فعالیت ضد میکروبی برای اسانس­های مینای ترکمنستانی و مینای کپت داغی به ترتیب در برابر باکتری­های میکروکوکوس لوتئوس، باسیلوس آتروفئوس و استافیلوکوکوس اورئوس مشاهده شد. اسانس مینای ترکمنستانی، هیچ اثر معنا داری را در برابر باکتری اشرشیا کلی نشان نداد. توصیه های کاربردی و صنعتی:نتایج نشان می­دهد که تاثیرات محیطی و نوع گونه­ های گیاهی بر مقدار و نوع اجزای فرار اسانس­ها تأثیر می­گذارد. همچنین، اثر ضد باکتریایی نشان داده شده توسط این اسانس­ها پایه ی علمی را فراهم کرده که استفاده از آن­ها در درمان­های دارویی معتبر می ­باشد. جداسازی و خالص سازی ترکیبات گیاهی مختلف، ممکن است ترکیباتی با خاصیت ضد میکروبی بیشتر را فراهم آورد. Manuscript profile -
Open Access Article
128 - فعالیت ضد میکروبی 5 گونه جلبک سبز - آبی و 3 گونه جلبک سبز جمع آوری شده از مشهد و حومه
سمانه رحیمی محمود ذکایی ندا سلطانیمقدمه و هدف: جلبک­ها دارای تنوع وگستردگی فراوانی هستند و روز به روز با توجه به ارزش دارویی و غذایی آن­ها مطالعات فراوانی بر روی خواص آن­ها انجام می شود. در تحقیق حاضر نیز فعالیت ضد میکروبی جلبک­های سبز- آبی و سبز جمع آوری شده از مشهد و حومه، مورد بررسی ق Moreمقدمه و هدف: جلبک­ها دارای تنوع وگستردگی فراوانی هستند و روز به روز با توجه به ارزش دارویی و غذایی آن­ها مطالعات فراوانی بر روی خواص آن­ها انجام می شود. در تحقیق حاضر نیز فعالیت ضد میکروبی جلبک­های سبز- آبی و سبز جمع آوری شده از مشهد و حومه، مورد بررسی قرار گرفتند .روش تحقیق: اثر عصاره­های آبی، اتری و متانولی، گونه های جلبک شناسایی­شده در برابر دو باکتری گرم مثبت و دو باکتری گرم منفی و دو قارچ مطالعه شد.نتایج و بحث: نتایج نشان داد که خاصیت ضدمیکروبی در دو گونه از خانواده­ی Nostocaceae ، یک گونه از خانواده­ی Chroococaceae ، یک گونه از خانواده­ی Heylaceae و یک گونه از خانواده­ی Scytonemataceae قابل تأمّل است. جلبک­های سبزِ شناسایی­شده، به 15 جنس از سه خانواده و پنج راسته متعلق بودند که فعالیت ضدمیکروبی آن­ها متعلق به دو گونه از خانواده­ی Scenedesmaceae و یک گونه از خانواده­ی Zygnemataceae بوده­است. مشاهده­ها حاکی از آن است که باکتری گرم مثبت Bacillus subtilis ، به وسیله­ی دو گونه جلبک سبز- آبی و یک گونه جلبک سبز ، مهار شد و باکتری گرم مثبت Staphylococcus epidermidis ، به وسیله­ی دو گونه از جلبک­های سبز – آبی و یک گونه جلبک سبز مهار شد، در حالی­که باکتری­های گرم منفی، نسبت به جلبک­های موجود، هیچ حساسیتی نشان ندادند. دو سیانو باکتری و یک گونه از جلبک­های سبز شناسایی­شده، رشد قارچ Candida albicans را مهار کردند ولی بر قارچ Candida kefyr هیچ تأثیری دیده نشد. می­توان گفت در این تحقیق باکتری­های گرم مثبت حساس­تر از گرم منفی نشان داده شده­اند.توصیه کاربردی/صنعتی: با توجه به نتایج حاصل از این تحقیق می توان از عصاره برخی از جلبک ها سبز-آبی و سبز جمع آوری شده از مشهد به عنوان ترکیبات ضد باکتریایی طبیعی استفاده کرد. Manuscript profile -
Open Access Article
129 - بررسی خصوصیات فیتوشیمیایی، ترکیبات معدنی و فعالیت های ضد میکروبی عصاره های اتیل استات و اتانولی Vernonia amygdalina و Morinda lucida در برابر برخی میکروارگانیسم های بیماری زا جدا شده بالینی: یک مطالعه مقایسه ای
ساویور یوسین اولواتویین دارمولا اولوابونمی اولوگ بنگا فیدلیکس ایوبامی اولایمی روتیمی ادمیلوا کریستینا آددوئین دارمولاBackground & Aim: Medicinal plants with antimicrobial activities have been used extensively in the West Africa regions. These plants of medicinal important have been proven to be very effective even where treatments with antibiotics failed. This study was aimed to e MoreBackground & Aim: Medicinal plants with antimicrobial activities have been used extensively in the West Africa regions. These plants of medicinal important have been proven to be very effective even where treatments with antibiotics failed. This study was aimed to evaluate the antimicrobial activities of Morinda lucida and Vernonia amygdalina leaves extracts on some pathogenic microorganisms isolated from clinical samples.Experimental: The antimicrobial activities were assayed using the agar well diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were evaluated using standard microbiological techniques. Phytochemical and mineral compositions of all the extracts were determined.Results: Phytochemicals such as saponins, tannins, flavonoids, glycosides, anthraquinone and alkaloids were present in both extracts of the plants with alkaloids having a higher percentage. Minerals such as K, Ca, Fe, Na and Fe were presented in appreciable quantities in both extracts of the plants with the K and Ca levels in the ethyl acetate and ethanol extracts of Morinda lucida significantly higher than that of V. amygdalina, while Na and Fe levels of Vernonia amygdalina was found to be higher than that of Morinda lucida. Heavy metals like Pb, Cd and As were presented in significant quantities in both extracts of the plants. All the extracts exhibited varying degrees of concentration-based antimicrobial activities against the tested pathogens. Both ethanol and ethyl acetate extracts of Morinda lucida showed a high significant antifungal activity against Trichophyton sp and Candida sp while the ethanol extract of Vernonia amygdalina showed a high significant antimicrobial activity against Klebsiella sp and Candida sp.Recommended applications/industries: The study provides information on the antimicrobial activities, phytochemical and mineral components of Morinda lucida and Vernonia amygdalina leaves. Also, it contributes to the development of alternative therapeutic agents against the pathogens tested in this study. Manuscript profile -
Open Access Article
130 - بررسی اثر ضدباکتریایی و فیتوشیمیایی عصاره متانولی و استونی گونههای Cupressus sempervirens و Juniperus excelsa بر برخی بیماریهای مهم غذایی
سیما یزدانی منیر دودی زهرا رضایتمند لادن رحیم زاده ترابیBackground & Aim: The unfettered usage of antibiotics has engendered a mounting resistance of bacteria to these agents, thereby necessitating the discovery and development of novel compounds and medications to a greater extent than previously. The focal point of thi MoreBackground & Aim: The unfettered usage of antibiotics has engendered a mounting resistance of bacteria to these agents, thereby necessitating the discovery and development of novel compounds and medications to a greater extent than previously. The focal point of this research was to explore the chemical constituents of acetone and methanol-extracted samples of Cupressus sempervirens and Juniperus excelsa, as well as their potential inhibitory actions against a prevalent food-borne pathogen.Experimental: This experimental investigation was conducted on standard strains of Staphylococcus aureus (PTCC 1430), Bacillus cereus (PTCC 1431), Listeria monocytogenes (PTCC 1298), Escherichia coli (PTCC 1399), and Shigella dysentery (PTCC 1188). Following the preparation of methanol and acetone extracts derived from Juniperus excelsa and Cupressus sempervirens using Clevenger apparatus, the antimicrobial efficacy was assessed by both qualitative agar well diffusion method and quantitative macrodilution method. The active constituents present in the methanol and acetone extracts of the plants were identified by gas chromatography-mass spectrometry (GC/MS) analytical method. Means were compared with Duncan's test at the 5% probability level.Results: The findings of this investigation pertaining to the antimicrobial potency of the extracts, ascertained via the qualitative agar well diffusion method, indicated its efficacy against Gram-positive strains including S. aureus and B. cereus. The methanol extracts of the J. Excelsa were found to produce smaller inhibition zones on the tested bacteria compared to other plant extracts. The highest sensitivity to the acetone extract of C. sempervirens and J. excelsa observed in S. aureus and B. cereus. The extracts obtained from the two plant did not demonstrate any discernible impact on the Gram-negative bacteria that are commonly associated with foodborne pathogens. The findings obtained through gas chromatography-mass spectrometry (GC/MS) indicated the presence of efficacious components such as Benzene 1,2,4,5-tetramethyl, and Cyclopropane cyclopenta in J. excels extract. The acetone extract of J. excelsa showed more potent antimicrobial constituents than its methanolic counterpart. It is anticipated that in forthcoming times, the acetone extract derived from this botanical specimen may be employed to prevent bacterial-induced foodborne illnesses.Recommended applications/industries: Duo to the existence of a range of bioactive compounds in the acetone extracts obtained from C. sempervirens and J. excels, these extracts have the potential to be used against the development of foodborne infections and diseases caused by bacterial agents. Manuscript profile -
Open Access Article
131 - ترکیبات شیمیایی و فعالیت ضدمیکروبی اسانس بومادران علیه باکتریها و قارچهای بیماریزای شایع در ماهی
میلاد عادل آرمین عابدین امیری مریم دیوبند رضا سفری الهام خلیلیمقدمه و هدف: با افزایش مقاومت عوامل بیماریزا در ماهی نسبت به داورهای شیمیایی، استفاده از گیاهان دارویی با خواص ضد میکروبی ضروری به نظر می­رسد. در این مطالعه ترکیبات شیمیایی بخش­های هوایی اسانس بومادران وحشی پرورش یافته در رشته­کوه­های زاگرس (جنوب غربی ای Moreمقدمه و هدف: با افزایش مقاومت عوامل بیماریزا در ماهی نسبت به داورهای شیمیایی، استفاده از گیاهان دارویی با خواص ضد میکروبی ضروری به نظر می­رسد. در این مطالعه ترکیبات شیمیایی بخش­های هوایی اسانس بومادران وحشی پرورش یافته در رشته­کوه­های زاگرس (جنوب غربی ایران) تعیین و خواص ضد میکروبی آن بر روی باکتری­ها و قارچ­های بیماریزای شایع در ماهی مورد بررسی قرار گرفت. روش تحقیق: در این مطالعه، خواص ضد میکروبی اسانس بومادران علیه پنج باکتری شامل استرپتوکوکوس اینیائی، یرسینیا راکری، ویبریو آنگوئیلاروم، آئروموناس هیدروفیلا و سودوموناس آئروجینوزا و چهار قارچ بیماریزای: ساپرولگنیا، فوزاریوم سولانی، کاندیدا آلبیکنز و آسپرژیلوس فلاوس مورد ارزیابی قرار گرفت. همچنین، ترکیبات تشکیل دهنده اسانس بومادران با استفاده از دستگاه گاز کروماتوگرافی- طیف سنج جرمی (GC/MS) تعیین شد. نتایج و بحث: بازده اسانس جمع آوری شده از استان چهارمحال و بختیاری 63/0% بود. مهمترین ترکیبات تشکیل دهنده اسانس بومادران شامل: 8-1 سینئول (2/25%)، کامفور (9/18%)، لینالول (9/6%)، بارنئول (7/5%) و آرتیمزیا (3/4%) بود. همه میکروارگانیسم­های مورد مطالعه نسبت به اسانس بومادران حساسیت بیشتری را نسبت به آنتی بیوتیک­های کنترل نشان دادند. بشترین خواص ضد باکتری و ضدقارچی بومادران به ترتیب علیه باکتری یرسینیا راکری (با قطر هاله مهار رشد 4/25 میلی­متر) و قارچ کاندیدا آلبیکنز (25 میلی­متر) مشاهده شد. این در حالی است که کمترین حساسیت نسبت به باکتری استرپتوکوکوس اینیائی (4/16 میلی­متر) و قارچ آسپرژیلوس فلاوس (14 میلی­متر) مشاهده شد. علاوه بر این، حداقل غلظت مهار کنندگی رشد (MIC) اسانس مورد مطالعه در محدوده 125 تا 800 میکروگرم/ لیتر تعیین شد که قادر به مهار رشد باکتری­ها و قارچ­های بیماریزای منتخب بود. توصیه کاربردی/صنعتی: نتایج این تحقیق نشان دهنده آن است که اسانس بومادران می­تواند به عنوان یک منبع بالقوه جدید و موثرتر از آنتی بیوتیک­های تجاری در صنعت آبزیان مورد استفاده قرار گیرد. هر چند که، انجام مطالعات بیشتر به منظور خالص سازی، جداسازی و تعیین ترکیبات دارای خواص ضد میکروبی اسانس بومادران ضروری به نظر می­رسد. Manuscript profile -
Open Access Article
132 - ترکیبات شیمیایی و فعالیت ضد میکروبی اسانس (Salvia officinalis L) و (Salvia virgata Jacq)
احمدرضا گلپرور امین هادی پناه محمدمهدی قیصری داوود نادری شیما رحمانیان مجتبی خرمیمقدمه و هدف : (Salvia officinalis L.) و (Salvia virgata Jacq) گیاهان چند ساله و معطر از خانواده نعناع (Lamiaceae) می­باشد. هدف از این تحقیق شناسایی ترکیبات شیمیاییو فعالیت ضد میکروبی دو گونه Salvia بود. روش تحقیق: قسمتهای هوایی S. officinalis و S. virgata به و Moreمقدمه و هدف : (Salvia officinalis L.) و (Salvia virgata Jacq) گیاهان چند ساله و معطر از خانواده نعناع (Lamiaceae) می­باشد. هدف از این تحقیق شناسایی ترکیبات شیمیاییو فعالیت ضد میکروبی دو گونه Salvia بود. روش تحقیق: قسمتهای هوایی S. officinalis و S. virgata به وسیله دستگاه کروماتوگرافی گازی متصل به طیف سنج جرمی (GC/MS) مورد تجزیه و شناسایی قرار گرفت. خاصیت ضد باکتریایی با استفاده از سویه باکتری­های استاندارد شامل Staphylococcus epidermidisوEscherichia coliو قارچ های Alternaria alternataوPenicillium funiculosumدردو روش دیسک دیفیوژن و چاهک گذاریمورد بررسی قرار گرفت. نتایج و بحث: نتایج بدست آمده از این تحقیق نشان داد که 42 ترکیب در اسانس S. officinalis و 29 ترکیب در اسانس S. virgata به ترتیب شناسایی شد. ترکیبات اصلی اسانس S. officinalis شامل α-thujone (18/37%)، 1,8-cineole (71/12%)، β-thujone (10/9%)، camphene (54/5%) و viridiflorol (33/5%) شناسایی شد. و ترکیبات اصلی اسانس S. virgata شامل caryophyllene oxide (23/30%)، β-caryophyllene (63/22%)، sabinene (82/11%)، 1-octan-3-ol (64/6%)، thujene (28/6%) و terpinene-4-ol (25/5%) شناسایی شد. اسانس S. officinalisو S. virgata در روش چاهک گذاری بیشترین ویژگی ضد میکروبی را برای S. epidermidisنشان داده و کمترین اثر را بر E. coli داشت. در روش دیسک دیفیوژن بیشترین ویژگی ضد میکروبی اسانس S. officinalisو S. virgata بر ضد E. coliوP. funiculosum (PTCC NO. 5301) بوده و کمترین اثر را بر A. alternate نشان داد. توصیه کاربردی/صنعتی: در مجموع اسانس S. officinalisخاصیت ضد میکروبی بیشتری نسبت به گیاهS. virgata نشان داد. Manuscript profile -
Open Access Article
133 - عملکرد اجزای اصلی در یک فرمول گیاهی به عنوان ماده محافظ ضدمیکروبی
محدثه محبوبی مریم معمار کرمانی حسن کرباسی زادهمقدمه و هدف: امروزه استفاده از مواد محافظ ضدمیکروبی شیمیایی به خاطر اثرات جانبی چالش برانگیز آن محدود شده است. در این مطالعه ما اثرات ماده محافظ ضدمیکروبی فرآورده با اجزای گیاهی (کرم کودک باریج) را در مقایسه با همان فرمولاسیون با متیل و پروپیل پارابن و یا با پتاسیم سورب Moreمقدمه و هدف: امروزه استفاده از مواد محافظ ضدمیکروبی شیمیایی به خاطر اثرات جانبی چالش برانگیز آن محدود شده است. در این مطالعه ما اثرات ماده محافظ ضدمیکروبی فرآورده با اجزای گیاهی (کرم کودک باریج) را در مقایسه با همان فرمولاسیون با متیل و پروپیل پارابن و یا با پتاسیم سوربات و سدیم بنزوات بررسی کردیم. روش تحقیق: مقایسه اثربخشی سه فرمولاسیون گیاهی حاوی مواد محافظ ضدمیکروبی مختلف در مقابل پاتوژنهای ایکولای، سودوموناس آئروجینوزا، کاندیدا آلبیکانس، آسپرژیلوس نایجر و استافیلوکوکوس اورئوس با استفاده از تست اثربخشی ماده محافظ ضدمیکروبی در طی 28 روز انجام گرفت. نتایج و بحث: کاهش دو لگاریتم در شمارش باکتریایی در روز چهاردهم از شمارش اولیه و عدم رشد تعداد باکتری و قارچ (کپک و مخمر) در روز بیست و هشتم نسبت به روز چهاردهم معیار قابل قبول برای پدیرش اثربخشی ماده محافظ ضدمیکروبی فرآورده های گروه دوم فارماکوپه آمریکا (USP) است. نتایج اثربخشی ماده محافظ ضدمیکروبی نشان داد که اجزای فعال گیاهی کرم کودک باریج معیارهای قابل قبولUSP را به مانند ماده محافظ ضدمیکروبی شیمیایی دارا می باشد. توصیه کاربردی/صنعتی: در طراحی یک فرمول گیاهی جدید ضروری است که اثربخشی فرآورده قبل از اضافه شدن مواد محافظ ضدمیکروبی شیمیایی ارزیابی شود. Manuscript profile -
Open Access Article
134 - بررسی تجزیه فیتوشیمیایی، محتوای فنولی و فعالیت آنتی اکسیدانی عصاره متانولی گیاه Caryopteris odorata D.Don.Robin
آرکانا جوشی آنیل پانت اَوم پراکاش مارکین استوکی والاری ایزیدورBackground & Aim:Caryopteris odorata is an aromatic shrub of family verbenaceae growing in tropical to subtropical regions. Various traditional applications of this plant have been documented guiding us to investigate the pharmacological activities and to further in MoreBackground & Aim:Caryopteris odorata is an aromatic shrub of family verbenaceae growing in tropical to subtropical regions. Various traditional applications of this plant have been documented guiding us to investigate the pharmacological activities and to further investigate the chemical composition. Experimental: Methanolic extract of aerial parts of Caryopteris odorata was investigated for antioxidant activity and the major phyto-constituents and total phenolic content were screened. The antioxidant activity was determined by measuring the scavenging activity using DPPH radical scavenging, NO radical scavenging, so radical scavenging activity, Metal chelating and reducing power assay. The antimicrobial efficacy was determined using agar well diffusion method against gram positive as well as gram negative bacteria. Sensitivity in terms of zones of inhibition and phytochemical composition of the extracts were also determined. In vitro anti-inflammatory activity was evaluated using albumin denaturation method. Diclofenec Sodium was used as a standard drug for the study of anti-inflammatory activity. Results: The results revealed that, methanolic extracts possess broad spectrum antibacterial activity against three microorganisms. Phytochemical analysis revealed the presence of diterpenes carbohydrates, glycosides, sterols and triterpenoids, aliphatic acids , tocopherols and phenolics. The methenolic extract exhibit strong antioxidant activity (IC50 values for DPPH assay 164.60 ±0.37 mg/ml, for metal chelating 280.30 ± 3.28 mg/ml, for reducing assay 80.38 ± 0.79 mg/ml, respectively). The extract also showed in vitro anti-inflammatory activity by inhibiting the heat induced albumin denaturation with IC50 value as 497.22 ± 1.34 mg/ml, respectively. From the result, it is concluded that the C.odorata possess a rich amount of different class of compounds and further the phytochemicals present in the C.odorata extract may be responsible for the antimicrobial, antioxidant and anti-inflammatory activity. Recommended applications/industries: The results obtained from various activities suggest Caryopteris odorata application as natural alternative antioxidant, anti-inflammatory and antimicrobial reagent. Manuscript profile -
Open Access Article
135 - فعالیت ضد میکروبی عصاره استونی Salvia officinalis در برابر جدایه های بیماریزا
قزلباش ق. ر پریشانی م. ر فویوانی م .حمقدمه وهدف: Salvia officinalis به عنوان یک داروی سنتی در درمان برخی از بیماریها در کشورهایی از جمله لبنان، سوریه، اردن و .... شناخته شده است. به علاوه S. officinalis در مطالعات مختلف برای بررسی تاثیرات دارویی مورد آزمایش قرار گرفته است. هدف در این مطالعه برر Moreمقدمه وهدف: Salvia officinalis به عنوان یک داروی سنتی در درمان برخی از بیماریها در کشورهایی از جمله لبنان، سوریه، اردن و .... شناخته شده است. به علاوه S. officinalis در مطالعات مختلف برای بررسی تاثیرات دارویی مورد آزمایش قرار گرفته است. هدف در این مطالعه بررسی فعالیت ضد باکتریایی این گیاه در برابر تعدادی از باکتریها بوده است. روش تحقیق: سه عصاره مختلف ( عصاره آبی، استون و اتانولی) از نظر ویژگیهای ضد باکتری در برابر باسیلوس آنتراسیس، باسیلوس سرئوس، اشریشیاکلی و استافیلوکوکوس اورئوس با روش انتشار دیسکی مورد ارزیابی قرار گرفتند. نتایج و بحث: عصاره های اتانولی تاثیرات ضد باکتریایی متوسطی را نشان دادند در حالیکه عصاره های استونی به خصوص عصاره برگها تاثیر قدرتمندی در این زمینه داشتند. از سوی دیگر عصاره های آبی هیچ فعالیت ضد باکتریایی در برابر باکتریهای مورد آزمایش نشان ندادند. نتایج نشان دهنده فعالیت بازدارندگی عصاره استونی با MIC معادل 10 میلی گرم در میلی لیتر برای باسیلوس آنتراسیس و MIC معادل 30 میلی گرم در میلی لیتر برای استاف اورئوس بود. میکروارگانیسمهای گرم منفی حساسیت بیشتری نسبت به عصاره ها نشان دادند. در مجموع می توان گفت که عصاره های استخراج شده توسط حلالهای آلی به خصوص استون می تواند به عنوان یک ترکیب ضدباکتری مورد استفاده قرار گیرد و نیز به این نکته می توان اشاره کرد که چنانچه قطبیت حلال کاهش می یابد ترکیبات ضد باکتری و بنابراین فعالیت عصاره افزایش نشان می دهد. توصیه های کاربردی/صنعتی: عصارهSalvia officinalisمی تواند به عنوان پماد برای درمان زخم استفاده شود و به عنوان یک داروی جدید در برابر باسیلوس آنتراسیس معرفی شود. Manuscript profile -
Open Access Article
136 - فعالیت ضد باکتری، ضد قارچی و ترکیبات شیمیایی اسانس مرزه تابستانی و مرزه خوزستانی
احمدرضا گلپرور محمد مهدی قیصری امین هادی پناه مجتبی خرمیمقدمه و هدف: هدف از این مطالعه بررسی فعالیت ضد باکتری، ضد قارچی و ترکیبات شیمیایی اسانس مرزه تابستانی (Satureja hortensis L.) و مرزه خوزستانی (Saturejakhuzestanica Jamzad) بوده است. روش تحقیق: فعالیت ضد باکتری و ضد قارچی اسانس بر ضد Staphylococcus aureus  Moreمقدمه و هدف: هدف از این مطالعه بررسی فعالیت ضد باکتری، ضد قارچی و ترکیبات شیمیایی اسانس مرزه تابستانی (Satureja hortensis L.) و مرزه خوزستانی (Saturejakhuzestanica Jamzad) بوده است. روش تحقیق: فعالیت ضد باکتری و ضد قارچی اسانس بر ضد Staphylococcus aureus و Candida albicans به ترتیبآزمایش شد. اسانس قسمتهای هوایی مرزه تابستانی و مرزه خوزستانی به وسیله دستگاه کروماتوگرافی گازی متصل به طیف سنج جرمی (GC/MS) مورد تجزیه و شناسایی قرار گرفت. نتایج و بحث: نتایج بدست آمده از این تحقیق نشان داد که 22 و 21 ترکیب در اسانس مرزه تابستانی و مرزه خوزستانی به ترتیب شناسایی شد. ترکیبات اصلی اسانس مرزه تابستانی شامل کارواکرول (56/36 درصد)، گاما ترپینن (24/75 درصد) و پ-سیمن (5/81 درصد) بوده و ترکیبات اصلی اسانس مرزه خوزستانی شامل کارواکرول (62/69 درصد)، گاما ترپینن (9/25 درصد) و پ-سیمن (8/36 درصد) شناسایی شد. نتایج بدست آمده از فعالیت ضد باکتری و ضد قارچی نشان داد که اسانس هر دو گیاه خاصیت ضد میکروبی دارد. MIC و MBC اسانس گیاه مرزه تابستانی و مرزه خوزستانی بر ضد S. aureus به ترتیب 1 /0 و 0/5 μl/ml و برایC. albicans 0/1 و 0/2 μl/ml تعیین شد. توصیه کاربردی/صنعتی: در مجموع ترکیبات اسانس گیاه مرزه خوزستانی می­تواند خاصیت ضد میکروبی بیشتری در مقایسه با مرزه خوزستانی داشته باشد و برای این منظور مورد استفاده قرار گیرد. Manuscript profile -
Open Access Article
137 - بررسی فعالیت ضد میکروبی اسانس آویشن شیرازی بر روی اسینتو باکتر بومانی جدا شده از نمونه های بالینی
خدیجه رضایی کیخواهی غلامرضا باقری مهدی حسن شاهیان سعیده سعیدیمقدمه و هدف: هدف از این مطالعه بررسی فعالیت ضد میکروبی اسانس آویشن شیرازی بر روی اسینتو باکتر بومانی جدا شده از نمونه های بالینی است. روش تحقیق: دوازده سویه اسینتو باکتر بومانی از بیمارستان های شهرستان زابل جداسازی شد. اسانس آویشن شیرازی با استفاده از دستگاه کلونجر بدس Moreمقدمه و هدف: هدف از این مطالعه بررسی فعالیت ضد میکروبی اسانس آویشن شیرازی بر روی اسینتو باکتر بومانی جدا شده از نمونه های بالینی است. روش تحقیق: دوازده سویه اسینتو باکتر بومانی از بیمارستان های شهرستان زابل جداسازی شد. اسانس آویشن شیرازی با استفاده از دستگاه کلونجر بدست آمد، در نهایت حداقل غلظت مهار کنندگی و حداقل غلظت کشندگی اسانس در برابر باکتریهای ذکرشده با روش میکرودایلوشن تعیین گردید. نتایج و بحث: نتایج حاصل از این مطالعه نشان داد که کمترین غلظت مهار کنندگی در برابر باکتری ها برابر با 31/0 میلی گرم بر میلی لیتر است و تنها یک سویه از باکتری در این غلظت مهار شده است. بیشترین غلظت مهار کننندگی برابر با 10 میلی گرم بر میلی لیتر بوده و یک سویه نیز در این غلظت مهار شده است. توصیه کاربردی و صنعتی: نتایج نشان داد که اسانس با افزایش غلظت اثرات ضد میکروبی آن افزایش پیدا می کند، اسانس آویشن فعالیت ضد میکروبی خوبی حتی در غلظت های پایین از خود نشان داد. با کاربرد اسانس آویشن شیرازی علیه باکتری های بیماریزا می توان به یک ماده ضد میکروبی خوب بدون اثر جانبی دست پیدا کرد. Manuscript profile -
Open Access Article
138 - Efficient Epoxidation of Alkenes using New Organometallic Catalysts ((E)-2,6-dimethoxy-4-((2-(5-methyl-1,3,4-thiadiazol-2-yl)hydrazono)methyl)phenol M: Cr, Fe, Co, Cu): An Antimicrobial and Theoretical Study of Catalyst
Rasha Mahmood Mohammed Abowd Asmaa Sabti -
Open Access Article
139 - سطح بالای موننسین برای گاوهای شیری در اواسط شیردهی:قابلیت هضم مواد مغذی، تخمیر شکمبه و سنتز پروتئین میکروبی
جی.ر. گاندرا جی.ای. فریتاس جونیور م. ماتورانا فیلهو ر.و. بارلتا ل.ن. رنو سی.اس. تاکیا ف.پ. رنوهدف از این مطالعه بررسی قابلیت هضم مواد غذایی، تخمیر شکمبه و سنتز پروتئین میکروبی در گاوهای اواسط دوره شیردهی بود که با سطح بالای موننسین تغذیه شدند. دوازده گاو هلشتاین به چهار مربع لاتین 3 × 3 توزیع شدند و به تیمار های: شاهد (CON)، موننسین 24 (M24، افزودن 24 میلی Moreهدف از این مطالعه بررسی قابلیت هضم مواد غذایی، تخمیر شکمبه و سنتز پروتئین میکروبی در گاوهای اواسط دوره شیردهی بود که با سطح بالای موننسین تغذیه شدند. دوازده گاو هلشتاین به چهار مربع لاتین 3 × 3 توزیع شدند و به تیمار های: شاهد (CON)، موننسین 24 (M24، افزودن 24 میلی­گرم موننسین/کیلوگرم ماده خشک جیره) و موننسین 48 (M48، افزودن 48 میلی­گرم موننسین/کیلوگرم ماده خشک جیره) تقسیم شدند. سطوح موننسین در جیره به طور خطی سبب کاهش مصرف ماده خشک بدون تغییر در قابلیت هضم ظاهری کل مواد مغذی شد. موننسین به طور خطی pH مایع شکمبه و غلظت استات را افزایش داد. موننسین به طور درجه دوم کل اسیدهای چرب با زنجیره کوتاه شکمبه، غلظت پروپیونات، استات و تولید پروپیونات و نسبت استات به پروپیونات را تحت تأثیر قرار داد. علاوه بر این، موننسین به طور خطی بازده سنتز پروتئین میکروبی را افزایش داد. Manuscript profile -
Open Access Article
140 - سلامت و عملکرد جوجه های گوشتی در پاسخ به شنبلیله و رازیانه
ع.ا. ساکی م. کلانتر نیستانکی ع. رحمت نژاد ف. میرزاآقاتباراثرات پودر شنبلیله و رازیانه بر فعالیت ضد میکروبی و عملکرد جوجه­های گوشتی مورد مطالعه قرار گرفت. بر اساس یک طرح کاملا تصادفی، 240 قطعه جوجه گوشتی (راس 308) به 4 تیمار و 4 تکرار اختصاص داده شد. تیمارها شامل کنترل (T1)، کنترل بعلاوه 5/0 درصد شنبلیله (T2)، کنترل بعلاوه Moreاثرات پودر شنبلیله و رازیانه بر فعالیت ضد میکروبی و عملکرد جوجه­های گوشتی مورد مطالعه قرار گرفت. بر اساس یک طرح کاملا تصادفی، 240 قطعه جوجه گوشتی (راس 308) به 4 تیمار و 4 تکرار اختصاص داده شد. تیمارها شامل کنترل (T1)، کنترل بعلاوه 5/0 درصد شنبلیله (T2)، کنترل بعلاوه 5/0 درصد رازیانه (T3) و کنترل بعلاوه 25/0 درصد شنبلیله و 25/0 درصد رازیانه (T4) بودند. جیره­ها در طول یک دوره 1 تا 42 روزه به پرندگان تغذیه شدند. نیمارهای حاوی گیاهان دارویی به طور معنی­داری عملکرد بهتری در مقایسه با گروه کنترل داشتند (05/0>P). همه تیمارها در مقایسه با گروه کنترل منجر به کاهش تعداد کل باکتریها شدند (05/0>P). تست حضور سالمونلا در همه نمونه­های منفی بود. وزن و طول روده کوچک و بازده لاشه در پرندگان تغذیه شده با گیاهان دارویی بیشتر از گروه کنترل بود (05/0>P). بنابراین استفاده از گیاهان داریی میتواند موجب بهبود عملکرد و شرایط سلامت پرنده گردد. Manuscript profile -
Open Access Article
141 - اثر تیمار کوتاه مدت با نانو ذرات نقره و محلول گلجایی ساکارز روی پیری گل و برگ شاخه بریده رز
خدیجه آل کثیر رحیم نقش بند حسنی علیرضا مطلبی آذراین آزمایش برای ارزیابی اثر تیمار کوتاه مدت نانو ذرات نقره در غلظت­ های صفر، 4، 25، 75 و 125 میلی­ گرم در لیتر روی گل شاخه بریدنی رز رقم ʼهای اند مجیکʻ بدنبال نگهداری در محلول ساکارز با غلظت­ های صفر، 2 و 3 درصد از نظر عمر گلجایی و برخی از صفات فیزیولوژیکی پ Moreاین آزمایش برای ارزیابی اثر تیمار کوتاه مدت نانو ذرات نقره در غلظت­ های صفر، 4، 25، 75 و 125 میلی­ گرم در لیتر روی گل شاخه بریدنی رز رقم ʼهای اند مجیکʻ بدنبال نگهداری در محلول ساکارز با غلظت­ های صفر، 2 و 3 درصد از نظر عمر گلجایی و برخی از صفات فیزیولوژیکی پس از برداشت آن انجام شد. این تحقیق به شکل آزمایش فاکتوریل در قالب طرح کاملا تصادفی با سه تکرار برای هر تیمار اجرا شد. آزمایش تحت شرایط دمای 2 ± 23 درجه سانتی­گراد، رطوبت نسبی 5±60 درصد و با شدت نوری محیط 12 میکرو مول در مترمربع درثانیه به مدت 12 ساعت توسط لامپ­ های فلورسنت انجام شد. نتایج نشان داد که تیمار 125 میلی­ گرم در لیتر نانو ذرات نقره همرا با ساکارز دو درصد و بدون ساکارز بیشترین تاثیر را در کنترل باکتری­ های انتهای ساقه را داشت. تیمار کوتاه مدت نانو ذرات نقره در کلیه غلظت­ ها سبب بهبود جذب محلول، حفظ رطوبت نسبی شاخه، بهبود باز شدن گل و شاخص فلورسانس کلروفیل برگ (Fv/Fm) در مقایسه با تیمار شاهد گردید. بیشترین میزان باز شدگی گل با درشت­ترین اندازه گل در تیمار 75 میلی­ گرم در لیتر نانوذرات همراه با ساکارز 2 درصد در روز نهم عمر گلجایی مشاهده گردید. کمترین میزان عمر گلجایی در تیمارهای آب مقطر به تنهایی و همراه با ساکارز بود. بیشترین میزان عمر گلجایی در تیمارهای 75 و 125 میلی­ گرم در لیتر نانو ذرات نقره بدون ساکارز و با ساکارز در مقایسه با سایر تیمارها مشاهده گردید. Manuscript profile -
Open Access Article
142 - بررسی اثر نانوسیلور، نانوسیل و پراکسید هیدروژن بر عمر پس از برداشت گل بریده رز (Rosa hybrida)
مونا شادباش فتح اله کشاورزشالبه منظور بررسی اثر نانوسیلور (5، 10 و 20 میلی­گرم در لیتر)، پراکسید هیدروژن (200، 400 و 600 میکرو مولار) و نانوسیل (200 ، 400 و 600 میکرو مولار) به عنوان ترکیبات ضدمیکروبی در محلول گلجایی گل بریده رز رقم "گراند پرس آنجلا"، آزمایشی با 10 تیمار و 3 تکرار در قالب طرح ک Moreبه منظور بررسی اثر نانوسیلور (5، 10 و 20 میلی­گرم در لیتر)، پراکسید هیدروژن (200، 400 و 600 میکرو مولار) و نانوسیل (200 ، 400 و 600 میکرو مولار) به عنوان ترکیبات ضدمیکروبی در محلول گلجایی گل بریده رز رقم "گراند پرس آنجلا"، آزمایشی با 10 تیمار و 3 تکرار در قالب طرح کاملا تصادفی انجام شد. گل­های بریده رز بصورت ممتد با این ترکیبات تیمار شدند. نتایج بیانگر تاثیر معنی­دار و مثبت نانوسیل بر صفات اندازه­گیری شده بود. بطوریکه بیشترین عمر گلجایی (16/13 روز)، بیشترین جذب محلول (943/0 میلی­لیتر در هر گرم وزن تر)، کمترین کاهش وزن تر (31/6 گرم)، کمترین باکتری ته ساقه (Log 10 CFU ml-1 67/87) و بیشترین کاروتنوئید گلبرگ (61/17 میکروگرم در هر گرم وزن تر) متعلق به تیمار 400 میکرومولار نانوسیل بود. بیشترین فعالیت آنزیم POD برای دو تیمار 5 میلی گرم در­لیتر نانوسیلور (94/3 نانومول در هر گرم وزن تر) و 400 میکرومولار نانوسیل (86/3 نانومول در هر گرم وزن تر) ثبت شد. غلظت­های مختلف نانوسیلور در حفظ عمرگلجایی و صفات وابسته به آن موفق نبودند که دلیل آن می­تواند استفاده ممتد از این ترکیبات باشد. غلظت­های مختلف پراکسیدهیدروژن نسبت به شاهد در صفات مورد ارزیابی برتر بودند. بطورکلی تیمار 400 میکرومولار نانوسیل به دلیل اینکه در تمامی صفات ارزیابی شده، جزو بهترین تیمارها بود و همچنین بیشترین عمر گلجایی را داشت، به­عنوان تیمار مناسبی جهت نگهداری طولانی مدت گل بریده رز توصیه می­شود. Manuscript profile -
Open Access Article
143 - اشکال محصولات زیست فعال گل زینتی- دارویی پریوش با خواص درمانی امیدبخش: یک مطالعه جامع از گسترش داروها بر پایه طبیعی
سوماشکارا راجاشکارا یوتپال باروپریوش (خانواده خرزهره) معمولا تحت عنوان "گل نامیرا"، "گل مرگ"، "بنفشه جادوگر" و "سمبل دوستی" نامیده می ­شود. این گیاه یک علف هرز شناخته شده در درمان عوارض گوناگون است. از اهداف این مطالعه، دستیابی به کاربردهای فایتوشیمی، فعالیت­ های دارویی، اتنوفارماکولوژی، تشخی Moreپریوش (خانواده خرزهره) معمولا تحت عنوان "گل نامیرا"، "گل مرگ"، "بنفشه جادوگر" و "سمبل دوستی" نامیده می ­شود. این گیاه یک علف هرز شناخته شده در درمان عوارض گوناگون است. از اهداف این مطالعه، دستیابی به کاربردهای فایتوشیمی، فعالیت­ های دارویی، اتنوفارماکولوژی، تشخیص، ترکیبات شیمیایی و زیستی گیاه پریوش است که باعث افزایش مدارک علمی در مورد خواص اتنوبوتانیکی این گیاه شده و به تشخیص خلاءهای موجود کمک کرده و نیازهای تحقیقاتی آینده را مشخص می­ کند. بیشتر استفاده ­های سنتی و سیستمیک که از عصاره این گیاه می­ شود، با مطالعات علمی ضد میکروبی، ضد سرطان، ضد قند خون، ضد سرطان خون، آنتی اکسیدانی، شیمی درمانی، التیام زخم، تولید نانوذرات، محصولات نانو و غیره به تایید رسیده است. محصولات جداسازی و خالص مثل آلکالوئیدهای ایندول ترپنوئیدی (TIA) شامل: آجمالایسین، آنهیدرووینبلاستین، کاتارانتین، سرپنتین، ویندولین، وینبلاستین، وینکریستین و ویندولینین قبلا تهیه، تایید و فعالیت آن­ ها به اثبات رسده است. این مقاله مروری به بررسی فعالیت ­های فایتوشیمیایی،اتنوفارکوماکولوژیکی، فارماکولوژیکی و زیستی گیاه پریوش می­ پردازند که مدرک معتبری در رابطه با داروهای تجاری باشد که با تحقیقات بیشتر بتوان داروهای مناسب­تری برای انواع درمان­ ها تولید کرد. Manuscript profile -
Open Access Article
144 - An Efficient In Vitro Propagation, Antioxidant and Antimicrobial Activities of Aphyllorchis Montana (Reichenb.f.)
Ganesan MahendranAn in vitro plant regeneration protocol was successfully established in Aphyllorchis Montana , a saprophytic achlorophyllous orchid by culturing immature seeds. Among the six basal media evaluated for seed germination, BM-TM medium was found to be the best followed by K MoreAn in vitro plant regeneration protocol was successfully established in Aphyllorchis Montana , a saprophytic achlorophyllous orchid by culturing immature seeds. Among the six basal media evaluated for seed germination, BM-TM medium was found to be the best followed by KC medium. After 40 days, all the media turned brown and the growths of the protocorms were arrested. Activated charcoal, 1 g/l in half strength BM-TM was found to be suitable for further development of protocorms. Half strength BM-TM medium was supplemented with different growth regulators either individually or in combinations for multiplication of shoots. Of the five cytokinins tested, TDZ at 6.8 μM was found to be most effective for multiple shoot induction yielding 17.24 ± 0.27 shoots after 10 weeks of culture. Addition of low concentration of NAA (1.3 μM) in MS medium supplemented with the cytokinin TDZ (6.8 μM) favoured shoot multiplication. A mean number of 27.56 ± 0.54 shoots with 3.92 ± 0.11 number of roots were produced per explant. The response of the seed derived protocorm to the different types of organic additives viz., peptone and yeast extract and coconut water was also evaluated. The addition of these organic additives to the medium containing TDZ enhanced the number of shoot regeneration. The plantlets were acclimatized in plastic pots containing sterilized vermiculite. The survival rate was 100 % when maintained in the culture room condition (25 ± 2 °C). Screening of the antibacterial, antioxidant activity and estimation of total phenolics and flavonoid content of methanolic extracts of micropropagated plants were also carried out and compared with that of the wild-grown plants. In all the tests, methanolic extract from wild-grown plants showed higher antioxidant, antimicrobial activity, total phenolics and flavonoid content than in vitro propagated plants. Manuscript profile -
Open Access Article
145 - Synthesis, physico-chemical properties and anti-bacterial activity of alginate nanogel encapsulated Artemisia diffusa extract
Farzaneh Shamsi Ali Asghar Bagheri Amir MirzaieOne of the new fields in nanotechnology is the use of nanogels to improve the release of drug and increase its antimicrobial effects. The aim of this study was to evaluate the antimicrobial effects of Artemisia diffusa extract encapsulated in hyaluronic acid nanogels ag MoreOne of the new fields in nanotechnology is the use of nanogels to improve the release of drug and increase its antimicrobial effects. The aim of this study was to evaluate the antimicrobial effects of Artemisia diffusa extract encapsulated in hyaluronic acid nanogels against the pathogenic bacteria including Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Pseudomonas aeruginosa. In this study, A. diffusa extract was first obtained by maceration method using aqueous solvent. The extract was then encapsulated in hyaluronic acid nanogels and the nanogels prepared were confirmed by scanning electron microscopy (SEM), Dynamic light scattering (DLS) and Fourier-transform infrared spectroscopy (FTIR) methods. The release of the extract was also investigated at different times, temperatures and pHs. Finally, the antimicrobial effects of nanogels encapsulated extract and free extract against the pathogenic bacteria were investigated by the lowest inhibitory concentration (MIC) method. The results showed that the synthesized nanogels had a spherical structure and an average size of 85 nm. FTIR results also showed that the extract was trapped within the nanogel structure. The release rate of the extract had a good pattern. Antimicrobial results showed that the nanogels encapsulated extract had lower MIC than the extract. Based on the findings of this study, it can be concluded that hyaluronic acid nanogels encapsulated extract have significant antimicrobial effects and it can be used in the pharmaceutical industry in the future with more studies. Manuscript profile -
Open Access Article
146 - Investigating the antimicrobial effects of textiles impregnated with ginger plant extract on some pathogenic microbes
Hamidreza Karimi Mohhamadreza Basiri Zahra DarabIn this article the antimicrobial effects and activity of the polypropylene fabrics used in medical textiles impregnated with ginger plant extract with the scientific name Zingiber officinale Rosc from the ginger family were investigated. For this purpose the fabrics ar MoreIn this article the antimicrobial effects and activity of the polypropylene fabrics used in medical textiles impregnated with ginger plant extract with the scientific name Zingiber officinale Rosc from the ginger family were investigated. For this purpose the fabrics are impregnated with aqueous, methanol and ethyl acetate extracts of ginger, and then the antimicrobial effect of the prepared fabrics on the microbes S. aureus, C. albicans, B. cereus, E. aerogenes, E. coli, B. subtilis, P. vulgaris were investigated. For anti-microbial tests, the growth inhibition halo diameter measurement method and disk blank were used. Each experiment had three simultaneous repetitions. For each repetition four petri dishes were considered. Statistical analysis of the results was done by comparing the mean of repetitions (Duncan's test). The results showed that ginger extracts have an inhibitory effect on all the microorganisms used in this research, and it can be considered an effective plant against microbes. Manuscript profile -
Open Access Article
147 - Evaluation of antifungal and antibacterial properties of palladium (II) Schiff base complex against common pathogenic strains between humans and aquatic
Mohammadreza Moghaddam-manesh Somaye Shahriari Sara HosseinzadeganMetal complexes containing Schiff base ligand are compounds that have biological effects such as antifungal, antibacterial, anti-malarial, anti-inflammatory, anti-viral and anti-fever properties. These compounds are also used in various industries. In this study, Pallad MoreMetal complexes containing Schiff base ligand are compounds that have biological effects such as antifungal, antibacterial, anti-malarial, anti-inflammatory, anti-viral and anti-fever properties. These compounds are also used in various industries. In this study, Palladium (II) Schiff base complex with the proposed formula C14H24N4O2Cl2Pd was synthesized by the method reported in Zabol University and used to investigate the antimicrobial effects. Antimicrobial activity such as antibacterial activity against pathogenic strains common between aquatic and human inclusive Loctococcus garvieae and Edwardsiella tarda strains and antifungal activity inclusive Aspergillus fumigatus and Candida albicans were performed according to CLSI standard and the results were reported based on inhibition zone diameter (IZD), minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFC). Based on the results, the studied complex showed MIC concentrations of 32 μg/ml and 64 μg/ml on the Edwardsiella tarda and Loctococcus garvieae, respectively. The MIC concentrations on Aspergillus fumigatus and Candida albicans, respectively, 512 μg/ml and 1024 μg/ml were observed. The results of this study showed that the effect of Pd (II) Schiff base complex has strong antimicrobial properties and has a better effect than some commercial drugs. Manuscript profile -
Open Access Article
148 - بررسی خاصیت سد خونی و ضد باکتریایی منسوجات با پوشش فلوئوروکربن و جنتامایسین
نیلوفر رفیع زاده زعیم کاوه کرمی -
Open Access Article
149 - In vitro production of recombinant ergokonin protein and its application in the polylactic acid film to control Aspergillus niger and Penicillium notatum
Mohammad javad Akbarian Meymand Arash BabaeiBackground & Objectives: Fungal is one of the most important causes of food spoilage. The use of antimicrobial active packaging increases food safety and shelf life. Hence, this study aimed to produce antifungal compounds (Ergoconin) and its application in the polyl MoreBackground & Objectives: Fungal is one of the most important causes of food spoilage. The use of antimicrobial active packaging increases food safety and shelf life. Hence, this study aimed to produce antifungal compounds (Ergoconin) and its application in the polylactic acid film to reduce the fungal contamination of Aspergillus niger and Penicillium notatum. Materials & Methods: To produce the Ergoconin recombinant protein, the target gene was entered into the pET21 expression vector, and the recombinant vector was transferred to Escherichia coli BL21 (DE3) as host of expression. Gene expression was evaluated by SDS-PAGE, western blot and dot blot. To purify the recombinant protein, a chromatography column was used. Poly-lactic acid bioactive films were prepared by casting method and adding different amounts of Ergoconin (0, 0.25, 0.5, and 0.75%) to 4% wt Poly-lactic acid solution. To investigate the antifungal effect of the poly-lactic acid film, Disc diffusion was used. Results: Ergokonin gene was replicated by specific primers. The expression of recombinant protein was performed by SDS-PAGE and confirmed by dot and western blot. Ergoconin compound had an antifungal effect on both fungi. The inhibition zone diameter for both fungi increased with increasing Ergoconin concentration. The antimicrobial film had a more antifungal effect on Penicillium notatum. Conclusion: Ergoconin destroys fungi by preventing the synthesis of glucan in the wall of fungi. Therefore, Poly-lactic acid film containing Ergoconin can be used as antimicrobial active packaging to increase food safety. Manuscript profile -
Open Access Article
150 - Study on antibacterial effect of marjoram (Origanum vulgare L) essential oil on bacteria causing citrus canker
Maryam Shahivand Mojtaba Rezaei Ahmadabadi Eidi Bazgir Rostam Yazdani BiokiBackground & Objectives: Citrus bacterial canker (CBC) disease is one of the main citrus diseases that is caused by Xanthomonas citri subsp. citri. The disease has been reported from several citrus- growing regions of Iran. Due to the high loss incurred by the disea MoreBackground & Objectives: Citrus bacterial canker (CBC) disease is one of the main citrus diseases that is caused by Xanthomonas citri subsp. citri. The disease has been reported from several citrus- growing regions of Iran. Due to the high loss incurred by the disease, its control is very much needed. One of the new methods to control the disease is the use of plant essential oils. This study was aimed to evaluate the effect of Marjoram essential oil on the activity of bacteria causing citrus canker. Materials & Methods: In this experimental study, CBC- causing bacteria were isolated from infected Poldokhtar citrus gardens, and subsequently identified by phenotypic and biochemical tests, along with specific primers. The essential oil was extracted using a Clevenger extraction device. Antimicrobial effect of the essential oil was evaluated using disc diffusion method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the essential oil were investigated, as well. Then, the effect of different concentrations of marigold essential oil was studied. Following Marjoram essential oil preparation, it was injected into a Gas-Chromatography Mass-Spectrometry (GC-MS) to determine the composition. Results: MIC and MBC of essential oil were observed as 3.5 µl/ml, and 4 µl/ml, respectively. The most effective concentration for disease prevention on cut leaves were 4 µl/ml , and 4.5 µl/ml, which reduced disease incidence by 62.5% and 53.12 % on water agar test, and trays method. Conclusion: It is concluded that marjoram essential oil can be used as a potential compound to control citrus bacterial canker disease. Manuscript profile -
Open Access Article
151 - Evaluation of antimicrobial potentiality and type-I polyketide synthase and nonribosomal peptide synthetase biosynthetic genes from some marine actinomycetes
Fatemeh shayesteh Nur Syuhana Binti Zakaria Gires Usup Asmat AhmadBackground and Objectives: Actinomycetes are commonly known as exceptionally prolific source of secondary metabolites with diverse biological activities. The aim of this study was to identify some actinomycete isolates from Malaysia marine environment and evaluate for t MoreBackground and Objectives: Actinomycetes are commonly known as exceptionally prolific source of secondary metabolites with diverse biological activities. The aim of this study was to identify some actinomycete isolates from Malaysia marine environment and evaluate for type-I polyketide synthase (PKS-I) and nonribosomal peptide synthetase (NRPS) genes as well as antimicrobial activity. Materials and Methods: Selected isolates were identified based on their morphology and molecular properties. PKS-I and NRPS genes were detected using specific primers and the potential of their antimicrobial activity was investigated by disc diffusion method.Results: The isolates varied morphologically on the basis of colony morphology, spore chain shape, aerial and substrate mycelium formation. Based on 16S rRNA gene sequences analysis, isolates Sdstm3k, Sdtm108 and Sdts4 were highly similar to Streptomyces sp. (95 %), whereas isolates Bvpd17e and SctgJI demonstrated highest similarity to Micrococcus sp. M2-19 (99 %) and Micrococcus leteus (95 %) respectively. While isolate Sdsb2k1a and Sdts46 were unidentified. The detection of PKS-I and NRPS genes revealed that only isolates SctgJI and Sdsb2k1a had both genes. Isolates Streptomyces sp. Sdst3k1 and Streptomyces sp. Sdts4 demonstrated the strongest and broadest spectrum of antimicrobial activity against 10 human pathogens tested. Conclusion: The present study indicated that actinomycetes isolated from marine environment in Malaysia can be a good source of the discovery of new bioactive compounds. Manuscript profile -
Open Access Article
152 - The predatory potential of Bdellovibrio bacteriovorus against clinically isolated pathogens with extensively drug-resistance (XDR)
Salman Odooli Rasoul Roghanian Giti Emtiazi Milad Mohkam Younes GhasemiBackground & Objectives: Bdellovibrio-and-like organisms (BALOs) are a group of predatory bacteria which invade other Gram-negative bacterial cells for growth. The bacteriolytic nature of Bdellovibrios make them one of the promising alternatives for conventional ant MoreBackground & Objectives: Bdellovibrio-and-like organisms (BALOs) are a group of predatory bacteria which invade other Gram-negative bacterial cells for growth. The bacteriolytic nature of Bdellovibrios make them one of the promising alternatives for conventional antibiotics. In this study, the isolation and molecular identification of Bdellovibrio bacteriovorus strain SOIR-1 was described. The antibiotic resistance pattern of some clinically isolated Gram-negative pathogens was determined, and the predatory potency of SOIR-1 toward them was evaluated. Material & Methods: Double-layer agar technique, transmission electron microscopy, and PCR targeting the Bdellovibrios-specific hit locus were used for the isolation, morphological investigation, and molecular identification of SOIR-1, respectively. Following the antibiotic resistance profile determination of clinical isolates, the bacteriolytic activity of SOIR-1 against them was evaluated through the plaque formation assay and lysis analysis in the broth co-cultures. Results: SOIR-1 was identified as a strain of Bdellovibrios bacteriovorus through the transmission electron microscopy examination and specific PCR detection. All clinical isolates showed the properties of extensively drug-resistant (XDR) and typical Bdellovibrios plaques were developed on their lawns of cells. The SOIR-1 had the highest and lowest predation efficiency among the clinical isolates toward Acinetobacter baumannii (84.33%) and Pseudomonas aeruginosa-369 (55.16%), respectively. Conclusion: This study highlights the great potential of SOIR-1 to prey and lyse XDR pathogens, regardless of their antimicrobial resistance state. So, B. bacteriovorus can be considered as a living antibiotic in the cases of infections caused by multidrug-resistant bacteria. Manuscript profile -
Open Access Article
153 - Spirulina: A healthy green sun with bioactive properties
Bahareh Nowruzi Mahnaz Jafari shaghayegh babaie Atena Motamedi AmirAli AnvarSpirulina is a photosynthetic cyanophyte that can grow in high intensity sunlight and very alkaline conditions and high temperatures. This algae contains vitamin supplements in the diet. Spirulina will be introduced as a complete food source in the near future. In fact, MoreSpirulina is a photosynthetic cyanophyte that can grow in high intensity sunlight and very alkaline conditions and high temperatures. This algae contains vitamin supplements in the diet. Spirulina will be introduced as a complete food source in the near future. In fact, one of the most important problems in the food industry is the use of synthetic preservatives and synthetic food additives that increase the risk of cancer. Therefore, efforts are being made around the world today to isolate new and safe antioxidants from natural sources. Among these, the natural products (secondary metabolites) of cyanobacteria are an important source of new drug compounds. Natural products not only have medicinal value themselves, but are also used as building models to create synthetic analogues. The chemical composition of Spirulina includes protein (70-55%), carbohydrates (25-25%), essential fatty acids (18%), vitamins, minerals and pigments such as carotene, chlorophyll a and phycocyanin. Because there is great potential for exploiting this algae and turning it into a dietary supplement in the production of a variety of food products such as cookies and biscuits, ice cream and cream cheese, and that in a small volume of this algae, it can be used as a golden key in nutrition. In this article, considering the potential potential of Cyanobacterium Spirulina, the morphological structure, life cycle, nutritional composition, antiviral activity, anti-cancer, anti-inflammatory, safety, anti-nephrotoxicity properties, increased visual acuity, weight loss and Blood lipids are treated. Obviously, the introduction of valuable properties of cyanobacteria Spirulina can be a suitable substitute for many antimicrobial compounds and synthetic antioxidants that not only pose no risk to the consumer, but can also improve consumer health. On the other hand, since the present study reviews the latest findings on cyanobacteria Spirulina, it is hoped that this study could pave the way for the introduction of edible microalgae with healing properties that can be used in the food industry. Manuscript profile -
Open Access Article
154 - Antimicrobial peptides of haloarchaea: Properties and applications of halocin
soheila Abbasi Giti EmtiaziThe high incidence of drug-resistant bacteria is currently a major global health concern, which, of course, calls for an immediate search for new antimicrobial mechanisms. Halocins synthesized by haloarchaea are usually stable at extremophile conditions and these featur MoreThe high incidence of drug-resistant bacteria is currently a major global health concern, which, of course, calls for an immediate search for new antimicrobial mechanisms. Halocins synthesized by haloarchaea are usually stable at extremophile conditions and these features have given them great potential in the field of biotechnology. In the present study, literature search was performed based on search of antimicrobial peptides derived from archaea, in several online research tools, such as Pubmed Medline, Scopus, Google scholar, Elsevier databases, Irandoc, Iranmedex, Magiran, SID and MEDLIB limited to the articles published between 1992 to 2021.The reason for the use of archaea antimicrobial compounds can be due to the mechanism of action and accuracy in identifying the target molecule, which has been proven in studies. But the production of pure halocin is very difficult due to the difficult techniques for culturing archaea and purification the active compounds produced.Antimicrobial peptides are attractive targets for drug development. Among the important uses of halocins, we can mention antimicrobial activity, preservative for salty food products, protection of tanned skin, prevention of heart damage, anti-cancer activity and as a tool for DNA absorption. Manuscript profile -
Open Access Article
155 - Synthesis of silver nanoparticles from Ephedra intermedia extract and evaluation of antibacterial and antioxidant properties
Mina Tetrontan Seyed Mohammad Mahdi Hamdi Maryam Ebrahimi TajabadiBackground & Objective: Ephedra intermedia species from the Ephedraceae family is a shrubby plant and is considered among the primitive plants. The aim of this study is to synthesize silver nanoparticles from the extract of this species in order to investigate its antim MoreBackground & Objective: Ephedra intermedia species from the Ephedraceae family is a shrubby plant and is considered among the primitive plants. The aim of this study is to synthesize silver nanoparticles from the extract of this species in order to investigate its antimicrobial and antioxidant effects. Materials and methods: First, methanolic extract was prepared and silver nanoparticles were synthesized using silver salt. A spectrophotometric device was used to verify silver nanoparticles and a scanning electron microscope was used to check its dimensions and shape. FTIR analysis was used to investigate the possible organic compounds involved in the synthesis of nanoparticles, and to determine the concentration of nanoparticles, the analysis was performed by AAS and the antioxidant properties were evaluated by the DPPH method. In order to evaluate the antimicrobial activity, MBC and MIC and disking method were used. Results: The nanoparticles produced were spherical and in the range of 30-89 nm, and the most effective group of agents that played a role in its production were the hydroxyl group (O-H) and alkene compounds (C=C), and the concentration of biosynthetic nanoparticles was 2.25 mg/liter indicates a high concentration of synthesized nanowires. The results of MIC and MBC tests were the same and its concentration was 2000 μg/ml. Conclusion: The results of this research showed that the biosynthetic nanoparticle obtained from Ephedra Intermedia is more effective in inhibiting the growth of bacteria than the commercial nanoparticle, so it can be used as an alternative in pharmaceutical, medical and disinfectant applications. Manuscript profile -
Open Access Article
156 - The Inhibitory Effect of Doxycycline- Silver Nanoparticle Conjugate on Brucella melitensis
Mohsen Ajalli Mojtaba Salouti Hamed Alizadeh Zahra Heydari Hossein Hamzehei Aram AlizadehBackground and Objective: Brucellosis is a zoonotic disease caused by Brucella bacteria. According to recent studies, antimicrobial activity of silver nanoparticle was approved. The aim of this study was to investigate the antimicrobial activity of Doxycycline - silver MoreBackground and Objective: Brucellosis is a zoonotic disease caused by Brucella bacteria. According to recent studies, antimicrobial activity of silver nanoparticle was approved. The aim of this study was to investigate the antimicrobial activity of Doxycycline - silver nanoparticles conjugate against Brucella meltensis 16M. Material and Methods: After preparing the doxycycline-silver nanoparticle conjugate, its antimicrobial activity against Brucella meltensis 16M was determined by Well Diffusion Agar method in Muller Hintone Agar media. Also, Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Doxycycline-silver nanoparticle conjugate was determined by Macrodilution method in Muller Hintone Broth media. Finally, antibacterial effect of the nanoparticle was assayed in animal model. Results: The results showed that Doxycycline-silver nanoparticle conjugate has antimicrobial activity against Brucella melitensis 16M in laboratory condition. In mouse model, the conjugate of Doxycycline-silver nanoparticle could decrease effectively the Brucella melitensis load in liver. Conclusion: This study demonstrated that Doxycycline-silver nanoparticle conjugate has synergistic effect on Brucella melitensis 16M and can be useful in treatment of brucellosis. Manuscript profile -
Open Access Article
157 - Preparation and Characterization of Novel Bionanocomposite Based on Tapioca Starch/Gelatin/Nanorod-rich ZnO: Towards Finding Antimicrobial Coating for Nuts
M. M. Marvizadeh A. Mohammadi Nafchi M. Jokar -
Open Access Article
158 - Anti-inflammatory and antimicrobial studies of biosensitive Knoevenagel condensate β-ketoanilide Schiff base and its Co(II), Ni(II), Cu(II) and Zn(II) complexes
Natarajan Raman Ramaraj Jeyamurugan Jeyasekaran Joseph -
Open Access Article
159 - Studies on inclusion complexes of 2-[Substituted arylideamino]-1, 3, 4-thiadiazino [6,5b]indole with β- Cyclodextrin
Sunakar Panda Jagat Tripathy -
Open Access Article
160 - DNA interaction and antimicrobial studies of novel copper (II) complex having ternary Schiff base
Natarajan Raman Thanasekaran Baskaran Abraham Selvan Ramaraj Jeyamurugan -
Open Access Article
161 - Thermodynamic, spectral and antimicrobial activity of inclusion complexes of acridone and its oxime with β-cyclodextrin
Swapna Sankar Nayak Sunakar Panda -
Open Access Article
162 - Spectroscopic evidence of Cu(II) and Zn(II) complexes having amino acid based Schiff base: A special emphasis on in vitro antimicrobial, DNA binding and cleavage studies
Natarajan Raman Abraham Selvan Arunagiri Sakthivel -
Open Access Article
163 - Biologically vital metal-based antimicrobial active mixed ligand complexes: synthesis, characterization, DNA binding and cleavage studies
Natarajan Raman Ramaraj Jeyamurugan Arumhgam Sakthivel Rajendran Antony -
Open Access Article
164 - A Review of the Chemical Composition of Essential Oils of Thymus Species in Iran
AHMAD REZA GOLPARVAR AMIN HADIPANAH -
Open Access Article
165 - Contents of Aerial Parts of Salvia leriifolia Benth
Zahra Hosseinpoor Mohsen Abadi Behnam Mahdavi Esmaeil Rezaei-Seresht -
Open Access Article
166 - Biological Activity and Efficient Synthesis of 3, 4-Dihydropyrimidin-2-(1H)-one/thione Derivatives
Farhad Hatamjafari -
Open Access Article
167 - The Novel Synthesis Route for 3-Poly (vinylbenzyl)-5,5-Dimethylimidazolidine-2,4-dione Nanofibers and Study of Its Antibacterial Properties
Bozorgmehr Maddah -
Open Access Article
168 - Evaluation of Antimicrobial Effect of Cinnamomum verum Methanolic Extract and Essential Oil: A Study on Bio-preservative in Ketchup Sauce
Anoosheh Sharifan Mona Shafiee Akram Tabatabaee -
Open Access Article
169 - The Effects of ZnOnanorodson the Characteristics of Sago Starch Biodegradable Films
R. Alebooyeh A. MohammadiNafchi M. Jokr -
Open Access Article
170 - Antimicrobial and Barrier Properties of Bovine Gelatin Films Reinforced by Nano TiO2
R. Nassiri A. MohammadiNafchi -
Open Access Article
171 - Antimicrobial, Physicochemical, Mechanical, and Barrier Properties of Tapioca Starch Films Incorporated with Eucalyptus Extract
M. Rojhan L. Nouri -
Open Access Article
172 - Isolation of Cerebroside from Gynura procumbens Leaves and Biological Activities of the Leaves Extracts
Md. Saddam Hossain Md. Maniruzzaman Md. Minzanur Chowdhuary Junaid Ahmed Md. Mizanur Badal Mohammad Yousuf -
Open Access Article
173 - Active Packaging Film Based on Lysozyme/Polyvinyl Alcohol / Alyssum Homalocarpum Seeds Gum
Anna Abdolshahi Leila Monjazeb Marvdashti Bahare Salehi Filippo Maggi Javad Sharifi-Rad -
Open Access Article
174 - Fabrication of Cassava Starch/Mentha piperita Essential Oil Biodegradable Film with Enhanced Antibacterial Properties
Mohammad Mehdi Marvizadeh Arash Tajik Vajihesadat Moosavian Nazila Oladzadabbasabadi Abdorreza Mohammadi Nafchi -
Open Access Article
175 - Evaluation of Antimicrobial Activity of Hydroalcoholic Extracts from Different Parts of Ferula assafoetida L. in Three Regions of South Khorasan Province
Batool Jalili Sakineh Saeidi-sar Nahid Masoudian Asghar Zarban Mohammad Hasan Namaei -
Open Access Article
176 - Synthesis and Antimicrobial Activity of 5-chloro-1-ethyl-2-methylimidazole-4-sulfonyl-8-Quinolinoxide
Oluwaseyi Ovonramwen Bodunde Owolabi Amowie Philip Oviawe Abiodun Falodun -
Open Access Article
177 - Elaeagnus angustifolia L. Whole Fruit Ethanolic Extract: Phytochemical Composition and Antimicrobial Effects
Leila Monjazeb Marvdashti Maryam Mohammadbeigi Samaneh Arab Arezoo Ebrahimi Arezou Rezaei Anna Abdolshahi -
Open Access Article
178 - Effect of Linear and Cyclic Lysine-Lysine-Tryptophan- Tryptophan -Lysine-Phenylalanine Antimicrobial Peptide on Sodium Dodecyl Sulfate Micelle as Cell Membrane Mimetic: Molecular Dynamics Simulation Study
S. Hassan Mortazavi Mohammad Reza Bozorgmehr Mohammad Momen Heravi -
Open Access Article
179 - The Effects of Nano Zinc Oxide Shape on Optical Characteristics of Tapioca Starch Films and In Vitro Escherichia coli Microbial Growth Kinetics
Naser Tamimi Abdorreza Mohammadi Nafchi Hamid Hashemi Moghadam Homa Baghai -
Open Access Article
180 - Enhancing the Antimicrobial Properties Copper Oxide Shell with the Magnetic Mesoporous Core- Shell
Seyed Kamal Rajabi Shabnam Sohrabnezhad -
Open Access Article
181 - Antibacterial and Antioxidant Properties of Colorant Extracted from Red Onion Skin
Sara Moosazad Peyman Ghajarbeigi Razzagh Mahmoudi Saeed Shahsavari Roghayeh Vahidi Ali Soltani -
Open Access Article
182 - Assesment of Antimicrobial Resistance Modes in Children with Pyelonephritis
Hulal Saleh Sahib Falah Mahdi Al-Khafaji Ahmed Ali Obaid -
Open Access Article
183 - Isolation and characterization of antimicrobial activities of native actinomycete strains from agricultural soils in Guilan province
Elham Amiri Mirsassan Mirpour Khosro Essazadeh Behnam RastiActinomycetes are known as the largest reservoir of natural antibiotics in the world. For this reason, due to their ability to produce various antibiotics and other compounds of therapeutic importance, they are considered the golden microorganisms of the 21st century. T MoreActinomycetes are known as the largest reservoir of natural antibiotics in the world. For this reason, due to their ability to produce various antibiotics and other compounds of therapeutic importance, they are considered the golden microorganisms of the 21st century. The purpose of this research is the isolation and molecular identification of actinomycetes with antimicrobial properties from agricultural soils in the native areas of Guilan province. Soil samples were collected from the southwestern agricultural areas of Guilan province. Serial dilution was used to isolate actinomycetes. Then the morphological, physiological, and biochemical identification of the samples was done and finally, the molecular identification of the isolates was done using 16S rRNA sequencing and phylogenetic analysis. Antimicrobial activity was investigated against pathogenic microorganisms. A total of 14 isolates were identified.2 isolates with more antimicrobial properties were selected. Based on the results of phylogenetic studies and 16S rRNA sequencing, Amycolatopsis roodepoortensis strain EA7 with 99.63% confidence, and Streptomyces microflaveus strain EA6 with 93.92% confidence were identified. The isolated bacteria had more antimicrobial activity against Gram-positive pathogenic microorganisms Staphylococcus aureus and standard sample Staphylococcus aureus PTCC 1112. This research is the first report on the identification of actinomycetes with antimicrobial properties in the agricultural soils of the southwestern regions of Guilan province located in the Alborz mountains. The identification of the rare strain of Amycolatopsis roodepoortensis strain EA7 from the northern regions of Iran makes the soils of these regions very valuable. Manuscript profile -
Open Access Article
184 - Characterization of hydrothermally grown pure and graphene added samarium oxide nano-structures as a potential antimicrobial agent
Abbas Bagheri Khatibani Somayeh Saadat Niavol samaneh Rasouli Jamnani Hosein Milani MoghaddamSeductive and impressive applications of samarium oxide based materials in semiconductor science, created an interest to synthesize and study their physical properties. Therefore, after preparing pure samarium oxide and graphene/samarium oxide powders through hydrotherm MoreSeductive and impressive applications of samarium oxide based materials in semiconductor science, created an interest to synthesize and study their physical properties. Therefore, after preparing pure samarium oxide and graphene/samarium oxide powders through hydrothermal technique; with use of X-Ray diffraction (XRD), field emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, and UV-Visible spectrophotometry; structural, optical, and morphological properties of the samples have been evaluated. Using XRD, lattice constants and some related structural parameters were determined. Variation of optical band gap has been calculated too, with aiding absorbance, The XRD data revealed prevailed cubic samarium oxide phase in both samples, even though a sharp diffraction peak of graphene was attended in graphene/samarium oxide sample. SEM, Raman, and FTIR analysis showed the effects of graphene addition on main lattice of samarium oxide. Furthermore, the decrease of optical band gap due to graphene was also notable. Manuscript profile
Sanad
Sanad is a platform for managing Azad University publications
Links
Related Centers
Technical Support
Official pages
The rights to this website are owned by the Raimag Press Management System.
Copyright © 2021-2024