Study of the antioxidant and antimicrobial effects of the ethanolic extract of Eucalyptus camaldulensis Dehnh against infectious bacteria isolated from clinical and animal sources
Subject Areas : Journal of Comparative PathobiologyMahdavi, S., Haj Azimian, S., Isa Zadeh, A.R., Babash pour, M., Shishehgar, R. . 1
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Keywords: Eucalyptus camaldulensis Dehnh, Ethanolic Extract, Antimicrobial, antioxidant,
Abstract :
Due to the increasing resistance of pathogenic bacteria to common antibiotics and antimicrobial agents, researchers are finding antimicrobial agents with plant origin as alternative drugs. Antioxidants are the main factors in neutralizing free radicals and prevent the spread of chronic diseases and destruction of many foods. After preparing the ethanolic extract from leaves of Eucalyptus camaldulensis Dehnh by maceration method, the antimicrobial effect of it was determined on Staphylococcus aureus and Escherichia coli isolated from women’s urogenital tract infections and food stuff and Salmonella pullorum and Salmonella gallinarum isolated from birds cases by minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) methods. The antioxidant and reducing power effects of ethanolic extract of Eucalyptus camaldulensis Dehnh were studied by DPPH(2,2-diphenyl-1-picryl-hydrazyl-hydrate) assay. The least to most antimicrobial effects of ethanolic extract of Eucalyptus camaldulensis Dehnh were seen against Salmonella pullorum, Escherichia coli, Staphylococcus aureus and Salmonellagallinarum, respectively. Antioxidant effect and reducing power of ethanolic extract of Eucalyptus camaldulensis Dehnh were recorded less than BHT (Butylatedhydroxytoluene) in equal concentration (p<0.05). Ethanolic extract of Eucalyptus camaldulensisDehnh leaves in high concentration has antimicrobial, antioxidant and reducing power, so it could be used to pharmaceutical industry for chemotherapy and disinfectants in control of human and animal diseases and as food preservatives. The aim of this investigation is study of the antioxidant and antimicrobial effects of the ethanolic extract of Eucalyptus camaldulensis Dehnh against infectious bacteria isolated from clinical and animal sources.