Antimicrobial activity of bacteriocin of lactic acid bacteria against betalactamase producing bacteria and evaluation of synergistic effect by some antibiotics

Subject Areas : microbiology

reyhaneh shirkavand ¹ , Fatemeh Noorbakhsh ² , sahar Honarmand Jahromi ³

1 - Department of Microbiology, Biological Science College, Varamin-pishva branch, Islamic Azad University,Varamin-Pishva,Iran
2 - Department of Microbiology, Biological Science College, Varamin-pishva branch,Islamic Azad University,Varamin-Pishva,Iran
3 - Department of Microbiology, Biological Science College, Varamin-pishva branch, Islamic Azad University,Varamin-Pishva,Iran

Received: 2024-06-18 Accepted : 2024-07-29 Published : 2025-02-08

Keywords: antimicrobial activity, bacteriocin, lactic acid bacteria, beta-lactamase, antibiotic,

Abstract :

Introduction: The purpose of this study was to investigate the antimicrobial activity of bacteriocins produced by lactic acid bacteria against beta-lactamase producing bacteria and to evaluate its synergism effect with some antibiotics. Method: In this study, bacteriocin was isolated from Lactobacillus acidophilus strains, and then the effect of produced bacteriocin against 3 pathogenic bacterial strains, Escherichia coli, Acinetobacter baumannii, and Pseudomonas aeruginosa was investigated by serial dilution method. The effectiveness of bacteriocin extract alone and in combination with commercial antibiotics against clinical pathogens was determined using the disk diffusion method in three replicates. Results: The results showed that all Pseudomonas aeruginosa and Acinetobacter baumannii strains were beta-lactamase positive. This study showed a statistically significant difference in bacteriocin-antibiotic synergism activity for ceftazidime, levofloxacin, and piperacillin in Pseudomonas aeruginosa strains and in Acinetobacter baumannii strains for tetracycline, gentamicin, and ceftazidime. Conclusion: Lactobacillus acidophilus bacteriocin along with some antibiotics showed synergism effect in beta-lactamase producing strains of Pseudomonas aeruginosa, Acinetobacter baumannii and Escherichia coli.

References:

1- Bush K, Bradford PA. β-Lactams and β-lactamase inhibitors: an overview. Cold Spring Harbor perspectives in medicine. 2016;6 (8).
2- Elander RP. Industrial production of beta-lactam antibiotics. Appl Microbiol Biotechnol. 2003;61(5-6):385-92.
3- Houbraken J, Frisvad JC, Samson RA. Fleming's penicillin producing strain is not Penicillium chrysogenum but P. rubens. IMA Fungus. 2011;2(1):87-95.
4- Tamma PD, Doi Y, Bonomo RA, Johnson JK, Simner PJ, RA ARLGTPDYB. A primer on AmpC β-lactamases: necessary knowledge for an increasingly multidrug-resistant world. Clinical Infectious Diseases. 2019;69(8):1446-55.
5- de Melo Pereira GV, de Oliveira Coelho B, Júnior AIM, Thomaz-Soccol V, Soccol CR. How to select a probiotic? A review and update of methods and criteria. Biotechnology advances. 2018;36(8):2060-76.
6- Cremon C, Barbaro MR, Ventura M, Barbara G. Pre-and probiotic overview. Current opinion in pharmacology. 2018;43:87-92.
7- Telhig S, Ben Said L, Zirah S, Fliss I, Rebuffat S. Bacteriocins to thwart bacterial resistance in gram negative bacteria. Frontiers in microbiology. 2020;11:586433.
8- Molujin AM, Abbasiliasi S, Nurdin A, Lee P-C, Gansau JA, Jawan R. Bacteriocins as potential therapeutic approaches in the treatment of various cancers: A review of in vitro studies. Cancers. 2022;14(19):4758.
9- Teame T, Wang A, Xie M, Zhang Z, Yang Y, Ding Q, et al. Paraprobiotics and postbiotics of probiotic Lactobacilli, their positive effects on the host and action mechanisms: A review. Frontiers in nutrition. 2020; 7:570344.
10- Kumariya R, Garsa AK, Rajput Y, Sood S, Akhtar N, Patel S. Bacteriocins: Classification, synthesis, mechanism of action and resistance development in food spoilage causing bacteria. Microbial pathogenesis. 2019;128:171-7.
11- Kashyap ML, Ganji S, Nakra NK, Kamanna VS. Niacin for treatment of nonalcoholic fatty liver disease (NAFLD): novel use for an old drug? Journal of clinical lipidology. 2019;13(6):873-9.
12- Zamfir M, Callewaert R, Cornea PC, Savu L, Vatafu I, De Vuyst L. Purification and characterization of a bacteriocin produced by Lactobacillus acidophilus IBB 801. Journal of Applied Microbiology. 1999;87(6):923-31.
13- Birla S, Tiwari V, Gade A, Ingle A, Yadav A, Rai M. Fabrication of silver nanoparticles by Phoma glomerata and its combined effect against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. Letters in Applied Microbiology. 2009;48(2):173-9.
14- Wang X, Zhang H, Chen X. Drug resistance and combating drug resistance in cancer. Cancer Drug Resistance. 2019;2(2):141.
15- Montalbán-López M, Cebrián R, Galera R, Mingorance L, Martín-Platero AM, Valdivia E, et al. Synergy of the Bacteriocin AS-48 and antibiotics against uropathogenic Enterococci. Antibiotics. 2020;9(9):567.
16- Rishi P, Preet Singh A, Garg N, Rishi M. Evaluation of nisin–β-lactam antibiotics against clinical strains of Salmonella enterica serovar Typhi. The Journal of antibiotics. 2014;67(12):807-11.
17- Cotter PD, Ross RP, Hill C. Bacteriocins—a viable alternative to antibiotics? Nature Reviews Microbiology. 2013;11(2):95-105.
18- Gut IM, Blanke SR, Van Der Donk WA. Mechanism of inhibition of Bacillus anthracis spore outgrowth by the lantibiotic nisin. ACS chemical biology. 2011;6(7):744-52.
19- Grazia SE, Sumayyah S, Haiti FS, Sahlan M, Heng NC, Malik A. Bacteriocin-like inhibitory substance (BLIS) activity of Streptococcus macedonicus MBF10-2 and its synergistic action in combination with antibiotics. Asian Pacific journal of tropical medicine. 2017;10(12):1140-5.
20- Biswas K, Upadhayay S, Rapsang GF, Joshi SR. Antibacterial and synergistic activity against β-Lactamase-producing nosocomial bacteria by bacteriocin of LAB isolated from lesser known traditionally fermented products of India. HAYATI Journal of Biosciences. 2017;24(2):87-95.
21- Singh AP, Prabha V, Rishi P. Value addition in the efficacy of conventional antibiotics by nisin against Salmonella. PLoS One. 2013;8(10):e76844.
22- Medeiros AA. Evolution and dissemination of β-lactamases accelerated by generations of β-lactam antibiotics. Clinical Infectious Diseases. 1997;24(Supplement_1):S19-S45.
23- Ulvatne H, Karoliussen S, Stiberg T, Rekdal Ø, Svendsen JS. Short antibacterial peptides and erythromycin act synergically against Escherichia coli. Journal of Antimicrobial Chemotherapy. 2001;48(2):203-8.
24- Ahmad V, Khan MS, Jamal QMS, Alzohairy MA, Al Karaawi MA, Siddiqui MU. Antimicrobial potential of bacteriocins: in therapy, agriculture and food preservation. International journal of antimicrobial agents. 2017;49(1):1-11.