بررسی تجزیه فیتوشیمیایی، محتوای فنولی و فعالیت آنتی اکسیدانی عصاره متانولی گیاه Caryopteris odorata D.Don.Robin

Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

آرکانا جوشی ¹ , آنیل پانت ² , اَوم پراکاش ³ , مارکین استوکی ⁴ , والاری ایزیدور ⁵

1 - گروه شیمی ، دانشکده علوم پایه و علوم انسانی ، دانشگاه کشاورزی و فناوری، ایالات متحده، اوتاراکند ، هند؛
2 - گروه شیمی ، دانشکده علوم پایه و علوم انسانی ، دانشگاه کشاورزی و فناوری، ایالات متحده، اوتاراکند ، هند؛
3 - گروه شیمی ، دانشکده علوم پایه و علوم انسانی ، دانشگاه کشاورزی و فناوری، ایالات متحده، اوتاراکند ، هند؛
4 - دانشکده جنگلداری، دانشگاه صنعتی، لهستان؛
5 - دانشکده جنگلداری، دانشگاه صنعتی، لهستان؛

Received: 2018-04-21 Accepted : 2019-07-02 Published : 2019-04-01

Keywords: Antioxidant, Antimicrobial, phytochemicals, Caryopteris odorata,

Abstract :

Background & Aim:Caryopteris odorata is an aromatic shrub of family verbenaceae growing in tropical to subtropical regions. Various traditional applications of this plant have been documented guiding us to investigate the pharmacological activities and to further investigate the chemical composition. Experimental: Methanolic extract of aerial parts of Caryopteris odorata was investigated for antioxidant activity and the major phyto-constituents and total phenolic content were screened. The antioxidant activity was determined by measuring the scavenging activity using DPPH radical scavenging, NO radical scavenging, so radical scavenging activity, Metal chelating and reducing power assay. The antimicrobial efficacy was determined using agar well diffusion method against gram positive as well as gram negative bacteria. Sensitivity in terms of zones of inhibition and phytochemical composition of the extracts were also determined. In vitro anti-inflammatory activity was evaluated using albumin denaturation method. Diclofenec Sodium was used as a standard drug for the study of anti-inflammatory activity. Results: The results revealed that, methanolic extracts possess broad spectrum antibacterial activity against three microorganisms. Phytochemical analysis revealed the presence of diterpenes carbohydrates, glycosides, sterols and triterpenoids, aliphatic acids , tocopherols and phenolics. The methenolic extract exhibit strong antioxidant activity (IC50 values for DPPH assay 164.60 ±0.37 mg/ml, for metal chelating 280.30 ± 3.28 mg/ml, for reducing assay 80.38 ± 0.79 mg/ml, respectively). The extract also showed in vitro anti-inflammatory activity by inhibiting the heat induced albumin denaturation with IC50 value as 497.22 ± 1.34 mg/ml, respectively. From the result, it is concluded that the C.odorata possess a rich amount of different class of compounds and further the phytochemicals present in the C.odorata extract may be responsible for the antimicrobial, antioxidant and anti-inflammatory activity. Recommended applications/industries: The results obtained from various activities suggest Caryopteris odorata application as natural alternative antioxidant, anti-inflammatory and antimicrobial reagent.

References:

Aberoumand, A. and Deokule, S.S. 2008. Comparison of phenolic compounds of some edible plants of Iran and India. Pakistan Journal of Nutrition, 7(4): 582-585.

Ajaib, M., Khan, Z., Khan, N. and Wahab, M. 2010. Ethnobotanical studies on useful shrubs of district Kotli, Azad Jammu & Kashmir, Pakistan. Pakistan Journal of Botany, 42(3): 1407-1415.

Arya, N., Prakash, O., Verma, A.K., Vivekanand and Pant, A.K. 2015. Variation in antioxidant potential of Curcuma longa L. collected from different ecological niches of western Himalayan region. International Journal of Pharmacy and Pharmaceutical Sciences, 7(7), 85-90.

Blois, M.S. 1958. Antioxidant determinations by the use of a stable free radical. Nature, 181: 1199-1200.

Liu, F. and Ng, T.B. 2000. Antioxidative and free radical scavenging activities of selected medicinal herbs. Life sciences, 66(8): 725-735.

Liu, H., Qiu, N., Ding, H. and Yao, R. 2008. Polyphenols contents and antioxidant capacity of 68 Chinese herbals suitable for medical or food uses. Food Research International, 41(4): 363-370.

Naskar, S., Islam, A., Mazumder, U.K., Saha, P., Haldar, P.K. and Gupta, M. 2010. In vitro and In vivo antioxidant potential of hydromethanolic extract of Phoenix dactylifera fruits. Journal of Scientific Research, 2: 144-157.

Pal, J., Singh, S.P., Prakash, O., Batra, M., Pant, A.K. and Mathela, C.S. 2011. Hepatoprotective and antioxidant activity of Zingiber chrysanthum Rosec. rhizomes. Asian Journal of Traditional Medicines, 6(6): 242-251.

Robak, J. and Gryglewski, R.J. 1988. Flavonoids are scavengers of superoxide anions. Biochemical pharmacology, 37(5): 837-841.

Sethi, S., Prakash, O., and Pant, A.K. 2015. Antioxidant Potential and Total Phenolic Content of Essential Oil and Various Extracts from Alpinia malaccensis (Burm. f.) Roscoe. Asian Journal of Chemistry, 27(11): 4085-4088.

Shahzadi, T., Abbasi, M.A., Riaz, T., Rehman, A.U., Siddiqui, S.Z. and Ajaib, M. 2011. Caryopteris odorata: A rich source of antioxidants for protection against chronic diseases and food products. Journal of the Chilean Chemical Society, 56(2): 678-681.

Shahzadi, T., Abbas, M.A., Aziz-Ur-Rehman, Riaz, T., Khan, K.M., Ahmad, V.U. and Ajaib, M. 2012. Characterization of chemical isolates of Caryopteris odorata. Journal of the Chemical Society of Pakistan, 34(2): 442-447.

Shetty, K., Curtis, O.F., Levin, R.E., Wikowsky, R. and Ang, W. 1995. Prevention of verification associated with in vitro shoot culture of oregano (Origanum vulgare) by pseudomonas spp. Journal of Plant Physiology, 147: 447-451.

Tundis, R., Loizzo, M.R., Menichini, F., Statti, G.A. and Menichini, F. 2008. Biological and pharmacological activities of iridoids: recent developments. Mini reviews in medicinal chemistry, 8(4): 399-420.

Wei, F., Jinglou, C., Yaling, C., Yongfang, L., Liming, C. and Lei, P. 2010. Antioxidant, free radical scavenging, anti-inflammatory and hepatoprotective potential of the extract from Parathelypteris nipponica Ching. Journal of Ethnopharmacology, 130: 521–528.

Woisky, R. and Salatino, A. 1998. Analysis of propolis: some parameters and procedures for chemical quality control. Journal of Apicultural Research, 37: 99-105.

Zhao, D.P., Matsunami, K. and Otsuka, H. 2009. Iridoid glucoside, (3R)-oct-1-en-3-ol glycosides, and phenylethanoid from the aerial parts of Caryopteris incana. Journal of Natural Product, 63: 241–247.