Preparation of new biocompatible polymeic nanofibers containing Daphne macronata plant extract and evaluation of its antimicrobial activity
Subject Areas : Environmental pollutions (water, soil and air)Laleh Safavinia 1 , Mohammad Reza Akhgar 2 , Batool Tahamipour 3 , Sayed Ali Ahmadi 4
1 - Department of Chemistry, Faculty of Science, Kerman Branch, Islamic Azad University, Kerman, Iran.
2 - Young Researchers and Elite Club, Sirjan Branch, Islamic Azad University, Sirjan, Iran. *(Corresponding Authors)
3 - Young Researchers and Elite Club, Sirjan Branch, Islamic Azad University, Sirjan, Iran
4 - Department of Chemistry, Faculty of Science, Kerman Branch, Islamic Azad University, Kerman, Iran.
Keywords: Daphne mucronata, Extract, Polyvinyl alcohol, Nanofibers, Antimicrobial.,
Abstract :
Background & Objective: Daphne macronata is one of the species of Daphne genus in Iran that has unique biological properties and has been used as a medicinal plant for a long time. In this study, biocompatible polymer nanofibers from polyvinyl alcohol and Daphne macronata plant extract were prepared as an antimicrobial coating with high efficiency by electrospinning method and its antimicrobial properties on gram-positive, gram-negative and the fungus was examined.
Material and Methodology: In this study, a polymer solution with weight / weight of 12% polyvinyl alcohol polymer was prepared and 2 mL of plant extract was added to 10 mL of polymer solution. In order to prepare nanofibers, the optimal conditions of the electrospinning device including voltage of 17 kV, solution injection rate of 3 mL / h, nozzle head to collector distance of 7 cm and collector speed of 100 rpm were set.
Findings: After preparing the polymer layer, the morphology and diameter of the nanofibers were determined by scanning electron microscopy and the average diameter of the nanofibers prepared under optimal conditions was 80 nm. Examination of the antimicrobial activity of this coating showed that this structure shows a significant antimicrobial effect on bacteria, so that the highest antimicrobial effect was observed on the gram-positive bacterium Streptococcus pyogenes with a growth inhibition zone diameter of 14 mm.
Discussion and conclusion: The nanocoatings obtained in this study can be used as an antimicrobial coating in the biological and medical fields for packaging or wound dressing due to their biocompatible, non-toxic, inexpensive and high efficiency structure.
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