Prevalence and antimicrobial susceptibility of Listeria monocytogenes in Koozeh cheeses of Urmia retails
Subject Areas :
Food Science and Technology
B. Abbasinejad
1
,
مسلم Neyriz-Nagadehi
2
,
Nagi Taher Talatappeh
3
1 - Graduated Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
2 - Assistance Professor of Food Hygiene and Quality Control Department, Urmia Branch, Islamic Azad University, Urmia, Iran
3 - Assistance Professor of Microbiology Department, Urmia Branch, Islamic Azad University, Urmia, Iran
Received: 2014-02-08
Accepted : 2015-06-23
Published : 2015-05-22
Keywords:
Listeria monocytogenes,
Antimicrobial susceptibility,
Koozeh cheese,
Urmia,
Abstract :
Koozeh cheese is of the local traditional cheeses of Azerbaijan and Kurdistan areas of Iran, and this cheese has high consumption among the people of these regions. Listeria monocytogenes is a food borne pathogen, and because of its growth at refrigeration temperatures has great concern in public health. In the present research, prevalence rate of L. monocytogenes in Koozeh cheeses of Urmia retails was surveyed. Moreover, antimicrobial susceptibility of the isolates was studied. One hundred samples of Koozeh cheese were collected randomly from Urmia retails in the spring of 2013. Samples were cultured in L-PALCAM Listeria selective enrichment broth with supplement. The enriched samples were transferred to PALCAM Listeria selective agar with antibiotics. For the identification of Listeria species, specific biochemical tests were performed. Antibiotic susceptibility of L. monocytogenes isolates was determined by disk diffusion method using penicillin G, chloramphenicol, ceftriaxone, erythromycin, tetracycline, and gentamicin. Among the 100 tested samples, L. monocytogenes was isolated from 3 of the samples. Antibiogram results showed that all L. monocytogenes isolates were susceptible to the tested antibiotics. However, tetracycline, penicillin and erythromycin were shown larger diameter of inhibition zone than the others. Although the prevalence rate of L. monocytogenes in Koozeh cheeses was low, its capability to growth in refrigerator conditions along with the pathogenicity of the bacterium to humans, hygienic production and distribution of these cheeses are recommended.
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Arsalan, S. and Ozdemir, F. (2008). Prevalence and antimicrobial resistance of Listeria spp. in homemade white cheese. Food Control, 19: 360–363.
Brooks, G.F., Carroll, K.C., Butel, J.C., Morse, S.A. and Mietzaner, T.A. (2013). Jawetz, Melnick & Adelberg’s Medical Microbilogy. 25th Edition McGraw-Hill Companiesm, pp. 187–195.
Carminati, D., Perrone, A., Giraffa, G., Neviani, E. and Mucchetti, G. (2004). Characterization of Listeria monocytogenes strains isolated from Gorgonzola cheese rinds. Food Microbiology, 21: 801–807.
Clinical and Laboratory Standards Institue (CLSI). (2011). Performance Standards for Antimicrobial Susceptibility Testing; Twenty First Informational Supplement. Document M100S21. Clinical and Laboratory Standards Institute, Wayne, PA, USA.
Comi, G., Cantoni, C., Valenti, M. and Civilini, M. (1990). Listeria species in Italian cheese. Microbiologie, Aliments, Nutrition, 8: 377–382.
Edalatian, M.R., Habibi- Najafi, M.B., Mortazavi, S.A., Alegría, A., Nassiri, M.R., Bassami, M.R., et al. (2012). Microbial diversity of the traditional Iranian cheeses Lighvan and Koozeh, as revealed by polyphasic culturing and culture-independent approaches. Dairy Science and Technology, 92: 75–90.
Fallah, A.A., Saei-Dehkordi, S.S. and Mahzounieh, M. (2013). Occurrence and antibiotic resistance profiles of Listeria monocytogenes isolated from seafood products and market and processing environments in Iran. Food Control, 34: 630-636.
Food and Drug Adminstration (FDA). (2011). Bacteriological Analytical Manual (BAM): Detection and Enumeration of Listeria monocytogenes in Foods. U.S. Food & Drug Administration, available online at: http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm071400.htm
Gillespie, S.H. and Hawkey, P.M. (Editors). (2006). Listeria and Erysipelothrix spp. in Principles and Practice of Clinical Bacteriology. 2nd Edition, John Wiley & Sons, Ltd, pp. 126-137.
Harakeh, S., Saleh, I., Zouhairi, O., Baydoun, E., Barbour, E. and Alwan, N. (2009). Antimicrobial resistance of Listeria monocytogenes isolated from dairy-based food products. Science of the Total Environment, 407: 4022–4027.
Jamali, H., Radmehr, B. and Lin-Thong, K. (2013). Prevalence, characterisation, and antimicrobial resistance of Listeria species and Listeria monocytogenes isolates from raw milk in farm bulk tanks. Food Control, 34: 121-125.
Korsak, D., Borek, A., Daniluk, S., Grabowsk, A. and Pappelbaum, K. (2012). Antimicrobial susceptibilities of Listeria monocytogenes strains isolated from food and food processing environment in Poland. International Journal of Food Microbiology, 158: 203–208.
Loncarevic, S., Danielsson-Tham, M. and Tham, W. (1995). Occurrence of Listeria monocytogenes in soft and semi-soft cheeses in retail outlets in Sweden. International Journal of Food Microbiology, 26: 245–250.
Pintado, C.M.B.S., Oliveira, A., Pampulha, M.E. and Ferreira, M.A.S.S. (2005). Prevalence and characterization of Listeria monocytogenes isolated from soft cheese. Food Microbiology, 22: 79–85.
Rudolf, M. and Scherer, S. (2001). High incidence of Listeria monocytogenes in European red smear cheese. International Journal of Food Microbiology, 63: 91–98.
Radostits, O.M., Gay, C.C., Hinchcliff, K.W. and Constable, P.D. (2006). Veterinary Medicine. 10th Edition, Saunders Elsevier, pp. 805-810.
Robinson, R.K. (Editor). (2002). Microbiology of Soft Cheeses in Dairy Microbiology Handbook. 3rd Edition, Jon and Wiley and Sons, Inc., New York, pp. 479-510.
Terplan, G., Schoen, R., Springmeyer, W., Degle, I. and Becker, H. (1986). Occurence, behaviour and significance of Listeria in milk and dairy products. Archiv für Lebensmittelhygiene, 37: 131–137.
Vanderzant, C. and Splittstoesser, D.F. (Editors). (1992). Listeria in Compendium of Methods for the Microbiological Examination of Foods. American Public Health Association, pp. 637-658.
Washington, W.J. and Stephen, A. (2006). Koneman’s Color Atlas and Textbook of Diagnostic Microbiology, 6th Edition, Lippinvott Williams & Wilkins, Philadelphia, pp. 765-773.
Yan, H., Neogi, S.B., Mo, Z., Guan, W., Shend, Z., Zhang, S., et al. (2010). Prevalence and characterization of antimicrobial resistance of foodborne Listeria monocytogenes isolates in Hebei province of Northern China, 2005–2007. International Journal of Food Microbiology, 144: 310–316.
Yu, T. and Jiang, X. (2014). Prevalence and characterization of Listeria monocytogenes isolated from retail food in Henan, China. Food Control, 37: 228-231.
