Listeria monocytogenes

Open Access Article

1 - Investigating the prevalence of resistance genes in Listeria monocytogenes isolated from food samples
Mohammad Reza Saebi Fahimeh nourbakhsh Hossein Khodabandeh shahraki

Listeria monocytogenes (L. monocytogenes) is a type of pathogenic bacteria that causes listeriosis infection. This facultative anaerobic bacterium is able to survive in the presence and absence of oxygen and is the cause of a wide range of diseases in humans and animals More

Listeria monocytogenes (L. monocytogenes) is a type of pathogenic bacteria that causes listeriosis infection. This facultative anaerobic bacterium is able to survive in the presence and absence of oxygen and is the cause of a wide range of diseases in humans and animals. Consumption of contaminated dairy products, meat and vegetables is the most important source of contamination. There are limited studies of the antibiotic resistance of Listeria monocytogenes species. Therefore, this study aims to evaluate the frequency and level of resistance in the evaluated samples. In this descriptive cross-sectional study, 150 different samples were randomly collected from different regions of Isfahan province. The samples included 60 samples of meat, 40 samples of dairy products (including milk, cheese, etc.) and 50 samples of vegetables (including leek, watercress, radish and basil). The serotyping of the isolated strains was done using the commercial O and H antisera of Listeria monocytogenes and according to the manufacturer's instructions, using slide agglutination method and antibiotic resistance evaluation. Standard PCR method was used to detect ermA, ermB, strA, tetS, tetA and ermC genes in the strains. Based on the serological reaction, somatic antigens O and flagella H of Listeria monocytogenes with the corresponding antisera, most Listeria species (70%) belong to serotype 1.2a and the rest from serotype 1.2b (19%) and 4b (11 %) They were. The results of the microbial investigation showed that the highest drug resistance was related to streptomycin (89%) and the lowest drug resistance in the evaluated isolates was related to ampicillin (14%) and chloramphenicol (13%). The most evaluated genes were related to strA gene and ermA gene, with frequencies of 79.8% and 65.4%, respectively. The prevalence of other Listeria monocytogenes genes evaluated in this study included tetA (17%), tetS (2.5%), ermB (10.7%) and ermC (2.1%). Manuscript profile

Open Access Article

2 - Study of Listeria monocytogenes strains’ prevalence in some local fish market of Shahr-e Kord, Iran
Esmail Pirali Kheirabadi Seyed Pezhman Hosseini Shekarabi

Contamination of seafood products to pathoges like Listeria is a for consumers. The aim of this study was to evaluate the field prevalence and typing of Listeria monocytogenes in the local fish market of Shahr-e Kord, Iran. Antibiotic susceptibility of the isolated List More

Contamination of seafood products to pathoges like Listeria is a for consumers. The aim of this study was to evaluate the field prevalence and typing of Listeria monocytogenes in the local fish market of Shahr-e Kord, Iran. Antibiotic susceptibility of the isolated Listeria was also carried out by the antibiotic disc diffusion method using 13 different antibiotics. A total of 100 rainbow trout were randomly collected from retail market in Shahr-e Kord for examination of the prevalence and typing of Listeria species using biochemical methods. Four isolates of Listeria were identified; One isolate of L. inovaii and 3 islolates of L. monocytogenes. All four isolates showed the highest antibiotic sensitivity (100 % susceptibility) to five antibiotics including cotrimoxazole, tetracycline, gentamicin, erythromosine, and vancomycin. However, all strains showed resistance to oxacillin. As L. monocytogenesis is of the most important pathogens in humans, fish contamination to different species of Listeria could be a potential risk and requires more attention. Manuscript profile

Open Access Article

3 - Detection and Determination of the Frequency of Listeria monocytogenes in Food samples by Molecular analysis of the hlyA gene
S. Ashrafi N. Bahmani F. Keshavarzi

10.30495/jftn.2023.72669.11245

Introduction: Listeriolysin (LLO) is a sulfhydryl-activated hemolysin bound to cholesterol, that is encoded by the hlyA gene of Listeria monocytogenes. The aim of this study was to detect and determine the frequency of Listeria monocytogenes in food samples through mole More

Introduction: Listeriolysin (LLO) is a sulfhydryl-activated hemolysin bound to cholesterol, that is encoded by the hlyA gene of Listeria monocytogenes. The aim of this study was to detect and determine the frequency of Listeria monocytogenes in food samples through molecular analysis of the hlyA gene.Materials and Methods: The present study was a descriptive cross-sectional assay conducted from the middle of October 2018 to the end of January of the same year. One hundred sixty-five samples of different types of meat, vegetables, hamburgers and shrimps were examined. Samples were randomly collected from restaurants, delicatessens, prepared food places, and different supermarkets. All samples were analyzed with phenotypic and biochemical tests. The samples suspected of Listeria monocytogenes, after DNA extraction, were evaluated by PCR with specific primers for the hlyA gene. Then the data and results were evaluated with using SPSS v.23 software and Excel software.Results: Phenotypic methods (microbial culture and biochemical investigation) confirmed 18 positive samples for Listeria monocytogenes, of which 10 (6.06%) samples were positive for Listeria monocytogenes by PCR of the hlyA gene. The rate of contamination in pounded kebab was 37.9%, chicken meat 4.4%, local hamburger 16.66% and lettuce 13.33%. Compared to other studies, these findings were more prevalent in some foods (e.g., local hamburger) and less in others (e.g., chicken meat).Conclusion: The use of biochemical methods for determining the frequency of Listeria monocytogenes in food samples in Sanandaj had a high percentage of false positives. Manuscript profile

Open Access Article

4 - The Antimicrobial Effect of Chitosan Nano Particles Film on Two Species of Food Bacteria
P. Rezaee روحا Kasra Kermanshahi

Introduction: Unbiodegradable materials used in food packaging and the microbial contaminants derived from this might create problems in our society. The application of biodegradable material that might have antimicrobial activity is desirable and is considered a priori More

Introduction: Unbiodegradable materials used in food packaging and the microbial contaminants derived from this might create problems in our society. The application of biodegradable material that might have antimicrobial activity is desirable and is considered a priority for the researchers. Chitosan is a biopolymer that eliminates these problems. Materials and Methods: In this study, chitosan nanoparticles and chitosan films were prepared and physical and chemical characteristics of these were determined and the antimicrobial effects against Staphylococcus aureus PTCC 1431 and Listeria monocytogenesusing different methods were assessed. Results: The size of chitosan nanoparticle was 273 nm with MIC and MBC of 3.5  for these bacteria. Synthezied chitosan film with 43.69 ± 2.4 mm of thickness, 1% of moisture content and 52% film solubility caused a reduction of 100% in the number of food pathogenic bacteria in 24 hoursat 30°C. Conclusion: Since chitosan is a biodegradable polymer and has antimicrobial activity in the form of chitosan nanoparticle and film against food pathogenic bacteria (Staphylococcus aureus PTCC 1431 and Listeria monocytogenes), it might be employed in the packaging industry particularly food.   Manuscript profile

Open Access Article

5 - The Effect of Sodium Caseinate Films Incorporated with Lactobacillus. acidophilus and Lactobacillus. casei Bacteria to Control Listeria monocytogenes Inoculated Silver Carp (Hypophthalmichthys molitrix) Fillet
S. M. Ojagh B. Shabanpour M. Kordjazi E. Abdolahzadeh M. Gharaei

Introduction: Casein-based edible films due to high nutritional value have potential toprotect the food products. The purpose of this investigation is to prepare sodium caseinatefilm containing Lactobacillus acidophilus and Lactobacillus casei and determine theantibacte More

Introduction: Casein-based edible films due to high nutritional value have potential toprotect the food products. The purpose of this investigation is to prepare sodium caseinatefilm containing Lactobacillus acidophilus and Lactobacillus casei and determine theantibacterial activity of the film against Listeria monocytogenes inoculated into the silver carpfillet.Materials and Methods: In this survey, Lactobacillus acidophilus and Lactobacillus caseibacteria in the form of solution were added to sodium caseinate film and dried at 40°C for 24hours. The effect of the film to control Listeria monocytogenes was determined on silver carpfillets and culture media during 9 days at 96 hour intervals. The viability of the LAB bacteriawithin 12 days in culture medium and fish fillet were studied at 96 hours intervals. The effectsof incorporated bacteria on the physical and mechanical properties of the film were alsoexamined.Results: The viability of lactic acid bacteria in fillets was increased. Both bacteria causedreduction in the Listeria count as compared to the control group. Lactic acid bacteria had asignificant effect on the solubility percentage, moisture, L index and color difference,however there were not significant differences in tensile strength and elongation percentage.Conclusion: Due to a significant decrease in L. monocytogenes viable count on culturemedia, it seems that the incorporation of LAB cells into films could be a useful method tocontrol food pathogens. Manuscript profile

Open Access Article

6 - Evaluating the growth potential of Listeria monocytogenes in Ready To Eat Vegetables
S. Shoja Gharehbagh A. Akhondzadeh Basti A. Khanjari A. Misaghi

10.30495/jfbt.2022.61634.10274

Manuscript profile

Open Access Article

7 - Identification of Listeria monocytogenes from milk by cultured and PCR gene actA
Mastaneh Gholami Mohsen Zargar soheil Aghaei

Foodstuffs are exposed to microbial contamination in different ways. Also، many pathogenic microbes are transferred to human beings thorough food. Listeria monocytogenes is a pathogenic microbe which is transferred to man via foods such as raw milk, vegetables especiall More

Foodstuffs are exposed to microbial contamination in different ways. Also، many pathogenic microbes are transferred to human beings thorough food. Listeria monocytogenes is a pathogenic microbe which is transferred to man via foods such as raw milk, vegetables especially cabbage. It can cause diseases involving meningitis, conjunctivitis, septicemia, mastitis and abortion. Regarding different reports on contamination of milk and its products by listeria monocytogenes in developing countries involving Iran، this study tries to examine contamination of milk and its products to this bacterium in Qom province. Different studies have proved the presence of pathogenic genes in listeria monocytogenes including actA، prF، plcA، plcB، hlyA and mpl. Because this bacterium has actin polymerization ability، it is able to move intracellularly and intercellularly، escape phagolysosome، resist humoral immune defense and eventually replicate in the host cell. actA protein is very important in controlling the interaction of actin filaments in peripheral cytoplasm of the host cell and causes the mobility of listeria monocytogenes. This study examines 100 samples including 100 raw milk samples by the isolation and molecular characterization of listeria monocytogenes. By enrichment and culture methods in selective agar plates، biochemical methods، serological confirmation and eventually by using CHORM agar and molecular confirmation based on actA genes، %3 of listeria monocytogenes contamination was confirmed and the presence of these genes was identified in this isolated strains Key words: listeria monocytogenes. actA gene، PCR، culture method. Manuscript profile

Open Access Article

8 - study the effect of silver nanoparticles against to biofilm in listeria monocytogenes
razieh ahmadinasab soheil Aghaei Mohammad Ali Gasemzadeh

Listeria monocytogenes has been isolated from many foods and may cause meningitis, septicemia and abortion in pregnant women. L. monocytogenes forms biofilm on many food contact surface materials and medical devices. Biofilm formation by this bacterium on many surfaces, More

Listeria monocytogenes has been isolated from many foods and may cause meningitis, septicemia and abortion in pregnant women. L. monocytogenes forms biofilm on many food contact surface materials and medical devices. Biofilm formation by this bacterium on many surfaces, is one of the most important problems in the food industry. Metal nanoparticles are effective antimicrobial compounds for controlling and removing of bacterial biofilms from non-biomaterial surfaces. The main purpose of this investigation was synthesis of silver iodide nanoparticles in-vitro condition and evaluation of their antimicrobial properties for inhibition of biofilm formation and biofilm eradication of Listeria monocytogenes(ATCC1298). Method: In this study nano-particles of silver iodide were synthesized respectively sonochemically and were confirmed using UV-Vis ,FTIR, EDX and Scanning Electron Microscopic procedures. Antimicrobial and antibiofilm properties of synthesized nanoparticles were determined using agar well diffusion and 96 well microplate dilution methods respectively. Results: Synthesized Silver nanoparticles had circular structure with 30 nm size. Antibiofilm activity and biofilm eradication of Iodide silver nanoparticles were obtained at 5 and 100 µg/ml respectively. Discussion: Based on obtained results, silver iodide nanoparticles can be used as the effective antimicrobial agents for inhibiting of biofilm formation of Listeria monocytogenes in the food industry. Manuscript profile

Open Access Article

9 - Detection of Listeria Monocytogenes in non-Pasteurized Milk in Kerman City by Phenotypic and Molecular Techniques
Somayeh Farahbakhsh Ashraf Kariminik

10.30495/qafj.2021.685560

Listeria monocytogenes is a gram-positive bacterium that causes listeriosis in humans and animals and is present in most foods including dairy and milk. Listeria monocytogenes can cause abortion and mastitis in cattle. In humans, as well as infections in pregnant women, More

Listeria monocytogenes is a gram-positive bacterium that causes listeriosis in humans and animals and is present in most foods including dairy and milk. Listeria monocytogenes can cause abortion and mastitis in cattle. In humans, as well as infections in pregnant women, fetuses, and a newborn baby are seen as meningitis septicemia. Despite these microorganisms in milk can be considered as a health indicator. In this study, 50 samples of raw and unpasteurized milk in the city of Kerman were collected and transferred to the laboratory by observing the cold chain. The culture was performed on a Listeria-specific culture medium and phenotypic identification was performed. For molecular identification, the DNA of bacteria identified by the phenotypic method was extracted using a commercial kit. Listeria monocytogenes was detected by using a special IGF kit to identify this bacterium from the Iranian Gene Fanavaran Company. The PCR product was electrophoresed with 1% gel and specific bands were observed. The results showed that 30 and 27 samples of unpasteurized milk showed contamination against Listeria monocytogenes based on the methods performed, culture, and PCR, respectively. This shows the attention and observance of hygienic conditions during the production and preparation of milk and the necessity of using pasteurized milk. Manuscript profile

Open Access Article

10 - The effect of nisin A and sodium benzoate on behavior of Listeria monosytogenes and some microbial and chemical parameters in silver carp (Hypophtalmichtys molitrix) fillet stored at 4˚C
رضا Safari M.R Saeidi Asl

   The effect of nisin A and sodium benzoate on Listeria monocytogenes, as well as some microbial (mesophilic, psychrotrophic and lactic acid bacteria) and chemical (peroxide and TVN) in silver carp (Hypophtalmichtys molitrix) fillet during storage period (0, More

   The effect of nisin A and sodium benzoate on Listeria monocytogenes, as well as some microbial (mesophilic, psychrotrophic and lactic acid bacteria) and chemical (peroxide and TVN) in silver carp (Hypophtalmichtys molitrix) fillet during storage period (0, 3, 6, 9 and 12 days) at 4˚C were evaluated. For this, Listeria monocytogenes (4 logCFU/g) was inoculated to the fillets and were dipped into 2% sodium benzoate solution for 15 min and left to stand for 10 min at 4˚C. Subsequently nisin A was added to the fillet (0/15 g/kg) and samples were kept at 4˚C while packaged in vacuum condition. The results showed that, application of nisin A and sodium benzoate decrease the number of Listeria monocytogenes from 4/12 to 3/66. However, in control groups the number of bacterium was increased from 4/43 to 5/14. Moreover, the number of mesophilic bacteria in treatment and control groups was increased from 4/39 to 6/79 and 4/48 to 7/93, respectively. The number of psychrotrophic bacteria in treatment and control groups was increased from 4/16 to 6/72 and 4/34 to 7/92, respectively. The similar result was achieved for lactic acid bacteria in which the number of these bacteria was increased from 2/74 to 4/08 and 2/9 to 4/78, respectively. Moreover, different peroxide value and TVN for treatment and control groups was achieved. In conclusion, application of nisin A and sodium benzoate showed different inhibitory effects on Listeria monocytogenes in culture media and silver carp. Manuscript profile

Open Access Article

11 - Effect of different concentrations of sodium chloride on the growth of Staphylococcus aureus and Listeria monocytogenes in salted Silver Carp (Hypophthalmichthys molitrix) fish fillets
نسرین Choobkar افشین آ Akhondzadeh Basti مهدی Soltani A.A Sari A.M Emami Rad منصوره Ghaeni لاله Roomiani

Salting of fish is a traditional method for fish preservation which reduces corruption, increase shelf life and is used in order to have an access to the new markets. In some countries, consuming semi-cooked or raw salted and smoked fish is well-liked. Due to the presen More

Salting of fish is a traditional method for fish preservation which reduces corruption, increase shelf life and is used in order to have an access to the new markets. In some countries, consuming semi-cooked or raw salted and smoked fish is well-liked. Due to the presence of halophilic microorganisms in salted fish, occurrence of food-borne infections is probable. The aim of this study was to investigate the antimicrobial activity of NaCl on Staphlococcus aureus and Listeria monocytogenes in salted silver carp. Effect of different concentrations of NaCl (4, 8, 12 %) on behavior of Staphlococcus aureus and Listeria monocytogenes in 10˚C during 3 weeks (0, 1, 2, 3, 6, 9, 12, 15, 18, 21 days) was determined by evaluation of the bacterial growth in salted fish fillets. Statistical analysis showed that application of different concentrations of NaCl had significant inhibitory effect on the growth of S. aureus and L.monocytogenes in salted fish fillets compared to control group (p<0/05). This study indicated that application of NaCl without any complementary element is not considered as a good preservative for extending the salted fish shelf-life. Consequently using of other natural preservatives along with salting is suggested. Manuscript profile

Open Access Article

12 - In vitro assessment of antimicrobial effect of methanolic extract of Peganum harmala against some important foodborne bacterial pathogens
طیبه zeinali محمد Mohsenzadeh رویا Rezaeian-Doloei رویا Nabipour

Foodborne bacterial pathogens play an important role in food infections/intoxications in human population. With ever increasing the number of antibiotic-resistant bacterial strains, there is an attempt to use the antimicrobial properties of herbs. Peganum harmala is a m More

Foodborne bacterial pathogens play an important role in food infections/intoxications in human population. With ever increasing the number of antibiotic-resistant bacterial strains, there is an attempt to use the antimicrobial properties of herbs. Peganum harmala is a medicinal plant of Iraniantraditional medicine which was used as an antiseptic in the past. Amongthe foodborne bacterial pathogens, Escherichia coli O157:H7, Salmonella Typhimurium and Listeria monocytogenes are considered as the most important and hazardous pathogens. The aim of this study was to evaluate the antibacterial effect of methanolic extract of Peganum harmala against these bacteria in vitro. The minimum inhibitory concentration (MIC) and minimum bactericidal concentrations (MBC) of methanolic extract of Peganum harmala was determined against three foodborne bacterial pathogens by micro-dilution method in Muller-Hinton broth. According to the results, MIC for E. coli O157:H7 and S. Typhimurium was 1.56 mg/ml. In the case of L. monocytogenes, it was estimated at 0.78 mg/ml. Moreover, results revealed that MBC for these organisms was similar to MIC concentrations. Regarding the results, Peganum harmala can be used as an ingredient in the formula of the disinfectants applied in the food systems.  Manuscript profile

Open Access Article

13 - Prevalence and antimicrobial susceptibility of Listeria monocytogenes in Koozeh cheeses of Urmia retails
B. Abbasinejad مسلم Neyriz-Nagadehi Nagi Taher Talatappeh

   Koozeh cheese is of the local traditional cheeses of Azerbaijan and Kurdistan areas of Iran, and this cheese has high consumption among the people of these regions. Listeria monocytogenes is a food borne pathogen, and because of its growth at refrigeration More

   Koozeh cheese is of the local traditional cheeses of Azerbaijan and Kurdistan areas of Iran, and this cheese has high consumption among the people of these regions. Listeria monocytogenes is a food borne pathogen, and because of its growth at refrigeration temperatures has great concern in public health. In the present research, prevalence rate of L. monocytogenes in Koozeh cheeses of Urmia retails was surveyed. Moreover, antimicrobial susceptibility of the isolates was studied. One hundred samples of Koozeh cheese were collected randomly from Urmia retails in the spring of 2013. Samples were cultured in L-PALCAM Listeria selective enrichment broth with supplement. The enriched samples were transferred to PALCAM Listeria selective agar with antibiotics. For the identification of Listeria species, specific biochemical tests were performed. Antibiotic susceptibility of L. monocytogenes isolates was determined by disk diffusion method using penicillin G, chloramphenicol, ceftriaxone, erythromycin, tetracycline, and gentamicin. Among the 100 tested samples, L. monocytogenes was isolated from 3 of the samples. Antibiogram results showed that all L. monocytogenes isolates were susceptible to the tested antibiotics. However, tetracycline, penicillin and erythromycin were shown larger diameter of inhibition zone than the others. Although the prevalence rate of L. monocytogenes in Koozeh cheeses was low, its capability to growth in refrigerator conditions along with the pathogenicity of the bacterium to humans, hygienic production and distribution of these cheeses are recommended. Manuscript profile

Open Access Article

14 - Effects of chiller on listeria monocytogenes contamination of poultry carcasses in industrial slaughterhouses of Western-Azarbaijan province
هیوا Karimi darhabi افشین Basti.A

Listeria spp. Has been isolated from poultry , red  meat products and fish in many countries around the world. Listeria monocytogenes has been isolated from water and ice used for relating freshness of food. Chiller water could possibly increase the microbial conta More

Listeria spp. Has been isolated from poultry , red  meat products and fish in many countries around the world. Listeria monocytogenes has been isolated from water and ice used for relating freshness of food. Chiller water could possibly increase the microbial contamination of poultry carcasses in slaughter-house. Therefore, the aim of this study was to investigate the effects of water chiller on Listeria monocytogenes contamination of poultry carcasses before and after chilling process in four industrial slaughter house of western Azarbaijan province was investigated. 180 poultry carcasses from 4 industerial slaughter house in western azarbaijan was investigated for Listeria monocytogenes befor and after chilling process using the modified Canadian version. All of the isolated belongs to the serogroup 1 and 4. Paired – samples T test indicated significant difference (P<0.05) between the contamination levels before and after chilling process. Manuscript profile

Open Access Article

15 - Effect of ginseng hydro-alcoholic extract along with aerobic exercise on antioxidant levels and inflammatory factors of cardiac tissue in male rat model of endocarditis caused by Listeria Monocytogenes (ATCC: 19114)
Seyed Zanyar Athari Younes Anzabi Zahra Karamouz Mir Alireza Nourazar

10.30495/jvcp.2022.1960383.1371

Endocarditis is a rare but serious infection following listeriosis. Ginseng extract has immunomodulatory effects. Regular exercise reduces the risk of chronic metabolic and cardiopulmonary diseases through its anti-inflammatory effects. This study aimed to evaluate the More

Endocarditis is a rare but serious infection following listeriosis. Ginseng extract has immunomodulatory effects. Regular exercise reduces the risk of chronic metabolic and cardiopulmonary diseases through its anti-inflammatory effects. This study aimed to evaluate the effect of ginseng extract against experimental endocarditis with Listeria monocytogenes in rats and the effect of aerobic exercise on improving its antimicrobial properties. For this purpose, 36 rats weighing 250±20 gr were randomly divided into six groups of 6, including sham, patient (receiving bacterial suspension), ampicillin treatment (15 mg/kg, s.c. for one month), ginseng treatment (0.025 mg/kg, i.p. for one month), aerobic exercise (60% of maximal oxygen consumption for one month) and ginseng+exercise (treated similarly to ginseng treatment and aerobic exercise groups). At the end of the study and after sacrifice, the levels of Total Antioxidant Capacity (TAC), Superoxide dismutase (SOD), Glutathione Peroxidase (GPx), and Paraoxonase-1 (PON-1), as well as the concentration of cardiac tissue cytokines including Interleukine-1 (IL-1), IL-6, IL-8, and TNF-α, were measured. Data were analyzed by ANOVA and Tukey post hoc test, and the significance level was considered as p<0.05. Tissue levels of antioxidants were significantly reduced in the patient group, while their levels were significantly increased in the ginseng+exercise group (p<0.05). On the other hand, tissue levels of IL-1, IL-6, IL-8, and TNF-α increased in the patient group, while their levels decreased significantly in the ginseng+exercise group (p<0.05). This study showed that hydro-alcoholic extract of ginseng along with aerobic exercise has a better effect than either of them alone against experimental endocarditis caused by listeria monocytogenesis. Manuscript profile

Open Access Article

16 - Evaluation of polyclonal antibody production level against Listeria monocytogenes in animal model
Yasin Bagheri Mansour Khakpour Hamid Zihag Alireza Barati

10.30495/jvcp.2019.669210

Listeria monocytogenes is a short, gram-positive, spore-free bacillus and facultative intracellular parasite, which is transmitted through contaminated vegetables, milk, cheese and meat to humans. Immunoglobulin Y (IgY) obtained from the egg yolk of immunized hens is a More

Listeria monocytogenes is a short, gram-positive, spore-free bacillus and facultative intracellular parasite, which is transmitted through contaminated vegetables, milk, cheese and meat to humans. Immunoglobulin Y (IgY) obtained from the egg yolk of immunized hens is a great and cheap source of polyclonal antibodies. These antibodies can be an appropriate replacement for antibodies produced in other laboratory animals. In recent years, IgY has been used widely in medical research for diagnosis, prevention and treatment of diseases. In current study, 12 Hy- line laying hens were divided into 2 groups of control (2 hens) and treatment (10 hens) and the treatment group was immunized by triple injection of Listeria monocytogenes antigens once a week. Then, after collecting the eggs and measuring total protein and yolk globulins at weeks 9,10 and 11 after the first injection, it was observed that there was no significant difference in both parameters at 9 and 10 weeks, while they showed a significant difference compared to the control group (p < /em><0.05). However, the amount of globulin and total protein of yolk in the eleventh week was significantly reduced although no significant difference was observed with the controls. Accordingly, it could be concluded that immunization of hens by antigens prepared from Listeria monocytogenes resulted in increased yolk globulin levels in the ninth and tenth weeks after injection therefore egg yolk can be used as a source for producing polyclonal antibodies against Listeria monocytogenes.          Manuscript profile

Open Access Article

17 - Case report of concurrent occurrence of coenurosis and listerial encephalitis in a sheep
Sirous Sadeghian Chaleshtori Mostafa Abdollahi Sara Shokrpoor Iraj Ashrafi-Tamai Maryam Hashemian

10.30495/jvcp.2020.1894379.1259

Coenurosis and Listerial encephalitis are two diseases of the central nervous system in sheep. In September 2018, an 18-month-old male sheep was referred to the Tehran University Veterinary Hospital with complaints of dullness, depression and anorexia. In observation an More

Coenurosis and Listerial encephalitis are two diseases of the central nervous system in sheep. In September 2018, an 18-month-old male sheep was referred to the Tehran University Veterinary Hospital with complaints of dullness, depression and anorexia. In observation and clinical examination within 1 hour, signs of dullness, depression, body temperature of 39°C , unilateral left sided blindness, clonic seizure, head deviation to the left, stiffness of the limbs, circling in a circular path and head tilt were recorded. After clinical examination, the patient underwent hematological laboratory tests and cerebrospinal fluid analysis, and autopsy under sterile conditions. Neutrophilic-eosinophilic pleocytosis was present in the cytological examination of cerebrospinal fluid and in autopsy, a protoscolex containing cyst was observed in the affected brain. The scolexes were examined by PCR (Polymerase Chain Reaction), which confirmed the presence of Coenuosis cerebralis. The brain sample was then subjected to bacterial culture (with cold enrichment) and histopathological examination. The presence of Listeria monocytogenes bacteria was identified during biochemical and PCR investigations. In histopathological study of brain tissue, meningoencephalitis and mononuclear cell infiltration around the cyst were observed. After bacterial isolation, the antibiotic susceptibility of isolates was determined with the highest susceptibility recorded to penicillin. In the survey of relevant herd status, the consumption of low quality silage with an acidity of 5.8 and the presence of 15 stray dogs in the pasture were recorded. Manuscript profile

Open Access Article

18 - The Effect of Oxidative Stress on Proteomics of Listeria monocytogenes PTCC 1297
Samaneh Kazemi Manoosh Zandehdel

10.30495/bioem.2022.696575

Listeria monocytogenes is a ubiquitous Gram-positive food-borne human bacterial pathogen that can cause listeriosis. This disease is fatal with a high rate of hospitalization (>90%). This study aims to determine the effect of hydrogen peroxide (H2O2) on L. monocytoge More

Listeria monocytogenes is a ubiquitous Gram-positive food-borne human bacterial pathogen that can cause listeriosis. This disease is fatal with a high rate of hospitalization (>90%). This study aims to determine the effect of hydrogen peroxide (H2O2) on L. monocytogenes PTCC 1297 proteomics. Bacterial cells exposed to gradually increasing sub-lethal concentrations of oxidative stress: 0.06, 0.3, 0.6, and 1.5 % of H2O2. Changes in the protein profile of cells exposed to H2O2 and control (non-adapted cells) were determined by isoelectric focusing (IEF) and sodium dodecyl sulfate– polyacrylamide gel electrophoresis (SDS-PAGE). Protein spots are visualized by staining with colloidal Coomassie Brilliant Blue. Based on the obtained results, one thousand four hundred spots were identified on the gels. Out of these points, 85 spots were reproducibly detected with the help of software and eye confirmation. After analyzing these points, 9 spots showed the most significant changes. These points had appropriate density and indicated the response of bacteria to stress conditions. It can be concluded that the structure of the proteome of L. monocytogenes PTCC 1297 changes when faced with oxidative stress. Nine protein spots on the gel were found to have substantial variations after protein spot analysis. These sites show how bacteria react under stress. Some of these spots' expressions had increased, while others had decreased. Manuscript profile

Open Access Article

19 - Evaluation of the chemical composition,antimicrobial and antioxidant of Potentilla recta extracts
hojjat azad Tooraj Mehdizadeh

The use of herbs has long been common in many societies. The importance of medicinal plants, with the intensification of the therapeutic effect, is that in many cases it can also avoid the toxicity and adverse effects of medication. In this study Potentilla recta, one o More

The use of herbs has long been common in many societies. The importance of medicinal plants, with the intensification of the therapeutic effect, is that in many cases it can also avoid the toxicity and adverse effects of medication. In this study Potentilla recta, one of the Iran and Azerbaijan region-native medicinal plants, was studied in terms of chemical composition, antimicrobial and antioxidant effect. After collecting, drying and milling of plants, the methanol, ethanol and ethyl acetate extracts obtained by maceration method. To identify the constituents of the extracts, gas chromatography connected tomass spectrometry was conducted. The DPPH radical scavenging method used to measure the antioxidant potential and the results was reported based on the IC50 index. After determine MIC and MBC using broth micro dilution method, agar well diffusion assay conducted to assess the antimicrobial effect of extracts. Data were analyzed by one-way analysis of variance (ANOVA) and means were compared using Duncan test at PL.monocytogenes microbial growth inhibition zone area (p <0.05). All prepared extracts had the ability to scavenging radical 1, 1-Diphenyl-2-picrylhydrazyl and the highest antioxidant potency after 30 minutes related to methanol extract. Most chemical compounds in methanol, ethanol and ethylacetate extracts, were respectively: methyllinoleate and ethyllinoleate. This study showed that the methanol extract of Potentilla recta has great antibacterial and antioxidant properties and with more research can be used in various applications. Manuscript profile

Open Access Article

20 - Bactericidal effect of sodium dodecyl sulfate (SDS) on some foodborne pathogens in ambient and refrigerator temperature
Siavash Maktabi Mehdi Zarei Roya Rostami

Sodium dodecyl sulfate (SDS) is one of the strong alkaline sulfates germicidal which is used in health and beauty purposes. In this study, the effect of minimum bactericidal concentration of SDS on 4 major foodborne pathogens including Escherichia coli, Salmonella typhi More

Sodium dodecyl sulfate (SDS) is one of the strong alkaline sulfates germicidal which is used in health and beauty purposes. In this study, the effect of minimum bactericidal concentration of SDS on 4 major foodborne pathogens including Escherichia coli, Salmonella typhimurium, Staphylococcus aureus and Listeria monocytogenes in normal saline was studied. Different concentration of SDS was made and based on standard methods the level of MIC and MBC for all strains was measured. Then the least bactericidal concentration of SDS on viability of strains in 4 and 25°C in different time's periods was studied. MIC and MBC results showed that E. coli and S. typhimurium have a similar sensitivity to SDS; meanwhile L. monocytogenes is so sensitive to SDS among the studied strains. The rate of viability of the strains is much higher in cold condition than in ambient temperature. SDS has an effective role in reducing the population of studied bacteria in different temperatures and time. Raising the temperature increases the antibacterial effect of the SDS. Also Listeria monocytogenes is very more sensitive to SDS in comparison to other studied bacteria. So according to the results of the study, sodium dodecyl sulfate could be useful for reducing population of pathogenic bacteria especially Listeria monocytogenes on surfaces, foodstuffs and equipment. Manuscript profile

Open Access Article

21 - Detection and antibiotic resistance pattern of Listeria monocytogenes strains isolated from curd and cheese
Rahil Kiyanpour Berjoee Hassan Momtaz Lida Lotfollahi zahra bamzaheh

10.30495/jfm.2022.1943942.1741

Listeria monocytogenes is a foodborne pathogen that causes listeriosis in humans and animals. Microbial contamination of food usually leads to widespread food poisoning in the form of widespread epidemics in the region, which is very significant in terms of public healt More

Listeria monocytogenes is a foodborne pathogen that causes listeriosis in humans and animals. Microbial contamination of food usually leads to widespread food poisoning in the form of widespread epidemics in the region, which is very significant in terms of public health and is one of the most important issues in developing countries. The aim of this study was to determine the contamination of Listeria monocytogenes in dairy products using culture method and final confirmation by PCR method. In this study, 150 different dairy samples offered in the market were purchased randomly. The samples were transferred to the laboratory under hygienic conditions and examined. In addition to culture medium experiments, positive samples were evaluated for final confirmation and identification of the pathogen by molecular PCR.14 (9.33%) positive samples including 6 samples of white cheese (4%), 4 samples of cream cheese (2.6%), and 3 samples of curd (2%) were positive for contamination. Antimicrobial susceptibility to 16 antibiotics was highly sensitive to clindamycin (47.37%). It is noteworthy that it was resistant to several drugs.The presence of Listeria monocytogenes in dairy products (cheese, cream cheese, and whey) was proven. Based on the results, people who consume contaminated dairy products are at potential risk of listeriosis. As a result, food safety authorities must establish an effective standard for examining the presence of Listeria in food. Manuscript profile

Open Access Article

22 - Evaluation of contamination of raw milk and traditional butter in Amol city with Listeria monocytogenes and determination of their antibiotic resistance.
Negin Najafi Mahdi Sharifi Soltani Atefeh Bozorgi Makerani

10.30495/jfm.2022.1945672.1748

Listeria is one of the most important bacteria that contaminates milk and milk products. The main species that causes listeriosis in humans and animals and is transmitted through contaminated food is Listeria monocytogenes. For this purpose, in order to identify Listeri More

Listeria is one of the most important bacteria that contaminates milk and milk products. The main species that causes listeriosis in humans and animals and is transmitted through contaminated food is Listeria monocytogenes. For this purpose, in order to identify Listeria monocytogenes in raw milk and traditional butter, in the spring of 1400, 40 samples of raw milk (20) and traditional butter (20) were collected from traditional dairy stores in Amol city. The samples were immediately transferred to a food microbiology laboratory in cold condition. The samples were first cultured in peptone water enrichment medium and then the enriched samples were transferred to Palkam Listeria selective agar medium with selective Listeria monocytogenes supplementation. Then antimicrobial susceptibility of Listeria monocytogenes isolates was determined by disk diffusion method using the antibiotics ampicillin, gentamicin, erythromycin, chloramphenicol, tetracycline, and ciprofloxacin. From the total samples, the prevalence of Listeria species in raw milk and butter was 35% and 10%, respectively. The results of antibiotic susceptibility and resistance test of raw milk samples showed that they were resistant to ampicillin 85.7% and tetracycline 71.4% and among butter samples only erythromycin was 100% resistant and ampicillin 100% semi-resistant. The mean of the obtained results in the antibiogram test were analyzed with 95% confidence and one-way analysis of variance. The results of the present study indicate the potential risk of infection due to consumption of raw milk and traditional butter to Listeria monocytogenes and raw milk had higher risk than butter. Manuscript profile

Open Access Article

23 - Study of the frequency of hlyAgene in Listeria monocytogenesisolated from Freshvegetables using PCR
Hossein Momeni Ali Sharifzadeh Masoumeh Bashiri

Abstract Listeria monocytogenesis an important psychotropic foodborne pathogen which may exist in fresh vegetables. Vegetables and their derivatives show a potential role in the transmission of Listeria to humans and epidemy of listeriosis.The objective of this study w More

Abstract Listeria monocytogenesis an important psychotropic foodborne pathogen which may exist in fresh vegetables. Vegetables and their derivatives show a potential role in the transmission of Listeria to humans and epidemy of listeriosis.The objective of this study was to assess the prevalence of L. monocytogenesand to find out the frequency of hlyAgene in raw vegetables in Shahrekord, Iran. This cross-sectional study was carried out on 145 samples collected from vegetables market outlets in Shahrekord. Isolation of L. monocytogeneswas performed using specific medium. PCR was performed using hlyAgene specific primers. Also, all of the 145 samples were tested directly using PCR Totally, 56% of the samples were positive for Listeria in culture method. However, based on PCR, 56% out of 145 samples were infected to Listeria. Furthermore, hlyAgene was found in 67% of Listeria. The results indicate that the frequency of L. monocytogenesin the vegetablessamples of this region is relatively high. Manuscript profile

Open Access Article

24 - مقایسه اثر تزریق و خوراکی گیاه آنیسون بر روی موشهای سوری نژاد Bal B/c مبتلا به بیماری لیستریوز
امین هاشم پور محمد حسین صادقی زالی صابر یوسفی

Background & Aim: Anise studies have demonstrated different properties such as anti-cancer, anti-fungal,   antibacterial, antioxidant, strengthen the liver, gastrointestinal tract,   wounds heal and strengthen the nerve cells. Outbreaks of listeriosis More

Background & Aim: Anise studies have demonstrated different properties such as anti-cancer, anti-fungal,   antibacterial, antioxidant, strengthen the liver, gastrointestinal tract,   wounds heal and strengthen the nerve cells. Outbreaks of listeriosis in the   general population are 7.0 per 100,000 populations. Pregnant women, infants,   the elderly and immunocompromised patients or those weaknesses comprise most cases. The aim of the present study was to investigate the   effect of intravenous and oral administration of anise herb to BALB/c race of mice with listeriosis.   Experimental: 2 units of listeria monocytogenes were prepared in 0.5 Mc Farland concentrations   and injected to BALB/c trace mouse intraperitoneal. Before and after   treatment, the mice were examined for agglutination test. Results:  The results of this study indicated that the effect of oral treatment with aqueous extract is better than injection of essential oil of anise.   Recommended applications/industries:  According to results of the present study, anise aqueous extract was recommended as a potential antibacterial agent against listeria monocytogenes. Manuscript profile

Open Access Article

25 - The frequency of ctpA gene in Listeria monocytogenes isolated from poultry using PCR
Soghra Maghsoudi Abbas Doosti Hashem Nayeri Mohamad Chehelgerdi

Background and Objectives: Listeria monocytogenes is able to cause infections in humans and around 50 species of animals. Meats and their derivatives show a potential role in the transmission of Listeria to humans  and epidemy of listeriosis. The aim of this study More

Background and Objectives: Listeria monocytogenes is able to cause infections in humans and around 50 species of animals. Meats and their derivatives show a potential role in the transmission of Listeria to humans  and epidemy of listeriosis. The aim of this study was to determine the contamination of poultry meats whit L. monocytogenes and to find out the frequency of ctpA gene. Materials and Methods: This cross-sectional study was carried out on 410 samples collected from poultry, including 110 fecal samples and 100 samples from each of liver, spleen and brain samples. Isolation of L. monocytogenes was performed using specific medium. PCR was performed using L. monocytogenes 16S rRNA and ctpA gene specific primers. Also, all of the 410 samples were tested directly using PCR. Results: Totally, 20.24% of the samples were positive for Listeria in culture method. The presence of Listeria in fecal, liver, spleen and brain specimens were 36.30%, 19%, 19% and 12%, respectively. However, based on PCR, 25.12% out of 410 samples were infected to Listeria. Furthermore, ctpA gene was found in 34.95% of Listeria. Conclusion: The results indicate that the frequency of L. monocytogenes in the poultry samples of this region is relatively high. PCR techniques make it possible to determine the L. monocytogenes infection directly in fresh samples.   Manuscript profile

Open Access Article

26 - Detection of actA gene in Listeria monocytogenes isolated from dairy products
Jamileh Norowzi Soheila Moradi Bidhendi Marzieh Shafiee

Background and Objectives: Listeria is a gram-positive facultative intracellular bacteria. The actA is one of the most important genes in this bacterium, which involves in bacterial movement in the host cell and so in its pathogenesis. The purpose of this study is to ev More

Background and Objectives: Listeria is a gram-positive facultative intracellular bacteria. The actA is one of the most important genes in this bacterium, which involves in bacterial movement in the host cell and so in its pathogenesis. The purpose of this study is to evaluate the actA gene in Listeria monocytogenes strains isolated from dairy products. Materials and Methods: This cross sectional study was performed on 70 samples of dairy products collected from Tehran and Babolsar, Iran from June to August 1391. The samples were grown onto BHI agar and Mueller agar. A PCR approach was used to detect the presence of the actA gene in the isolated Listeria species. Also, the isolated were grown into TSA containing 0.0015% Congo red in order to determine the invasive properties of L. monocytogenes. Results: This study showed contamination of the milk, cheese and soft cheese samples with L. monocytogenes (10 cases), L. innocua (4 cases), L. welshimeri (2 cases) and L. seeligeri (1 case). Furthermore, no yogurt and butter samples were contaminated with Listeria. Although all of these isolates contained actA gene in their genome, only 14% of the strains isolated from vegetables were positive for this gene. A total of 10 cases of isolated L. monocytogenes, 100% of the clinical strains, 70% of the strain food and 100% of standard strains purchased from Razi Institute were positive for Congo red phenotype. Conclusion: According to the results obtained in this study, detection of actA gene based on PCR can be used as an alternative approach for identification of pathogenic L. monocytogenes in samples without culture method. Also, due to the widespread use of dairy by individuals, it seems necessary to reduce bacterial contamination monitoring the production process, transport and distribute this material. Manuscript profile

Open Access Article

27 - ردیاپی پادتن ضد لیستریا مونوسیتوژنز با روش لاتکس اگلوتیناسیون در اسبان جنوب شرق ایران
پونا فرامرزپور احسان اله سخائی مهدی گلچین بلال صادقی

لیستریوز یک بیماری مشترک میان انسان و طیف وسیعی از دامهای اهلی، وحشی و پرندگان می باشد. هدف از انجام مطالعه ی حاضر ردیاپی پادتن ضد لیستریا مونوسیتوژنز با روش لاتکس اگلوتیناسیون در اسبان جنوب شرق ایران می باشد. برای این منظور تعداد 163 نمونه سرم از اسب های به ظاهر سالم ب More

لیستریوز یک بیماری مشترک میان انسان و طیف وسیعی از دامهای اهلی، وحشی و پرندگان می باشد. هدف از انجام مطالعه ی حاضر ردیاپی پادتن ضد لیستریا مونوسیتوژنز با روش لاتکس اگلوتیناسیون در اسبان جنوب شرق ایران می باشد. برای این منظور تعداد 163 نمونه سرم از اسب های به ظاهر سالم باشگاه های سوارکاری استان های یزد و کرمان تهیه شد. نتایج مطالعه حاکی از حضور پادتن در 34 نمونه از مجموع 163 سرم  مورد بررسی  (85/20 درصد) می باشد. بر این اساس به نظر می رسد که لاتکس اگلوتیناسیون آزمایش مناسبی برای غربالگری اولیه به منظور تشخیص بیماری می باشد Manuscript profile

Open Access Article

28 - Study of two human infectious bacteria in the kapoors(common carp) intestine in province khorasan razavi
امیر صفاری محمدحسین غفوریان

Incidence of food andndash;borne diseases caused by marine product has been increased in recent years. the aim of this study is to evaluate the late of contamination to listeria monocytogenes and E.coli o157H7. In fishes of Khorasan Razavibefore marketing.Forty fish sam More

Incidence of food andndash;borne diseases caused by marine product has been increased in recent years. the aim of this study is to evaluate the late of contamination to listeria monocytogenes and E.coli o157H7. In fishes of Khorasan Razavibefore marketing.Forty fish samples were taken from 10hatchery then alimentary tract of fishes were cultured in related medias. Isolation of listeria monocytogenes was done by cold- Enrichment method for seven days in 4oc in L.E.B. and suspected colonies transferred in to oxford agar .first identification of this bacteria was done by morphological and biochemical rests. Among suspiuons colonies, 5% were positive for listeria monocytogenes. Isolation of E. coli O157H7 in this regard first the digestion of the fisher were taken out in aseptic condition and after mixing that with brian heart infusion broth wich is a general rich environment after 24 hours selected rich environment of E.Coli , M.Cbroth ,being added , after growth of colony related to E.Coli , from E.M.B typic colony ,peptone water ,B.G broth , T.S.I being added. among suspicions colonies 75% rate of colony suspicious samples of E. coli infection were positive Gypsy and none to serotype O157H7 observe . These rates of contamination might refer to high pollution of pisiculture pools and low temperaturein Khorasan Razavi province Manuscript profile

Open Access Article

29 - Pathogenicity and Spontaneous Abortion caused by Listeria monocytogenes: a brief report
Manoosh Zandehdel Samaneh Kazemi Amirreza Hajati Ziabari

Listeria monocytogenes, as one of the foodborne pathogens, is a causative agent of listeriosis. Listeria is ubiquitous in the environment and can produce biofilms in the food production environment and thus contaminate ready-to-eat (RTE) products, which are typically co More

Listeria monocytogenes, as one of the foodborne pathogens, is a causative agent of listeriosis. Listeria is ubiquitous in the environment and can produce biofilms in the food production environment and thus contaminate ready-to-eat (RTE) products, which are typically consumed raw or without further processing. L. monocytogenes is an adaptable environmental bacterium. One of the most important characteristics of L. monocytogenes is its cold tolerance and resistance to high salt concentrations and low pH. On the other hand, this bacterium is an important pathogen in pregnant women, neonates, elderly individuals, immunocompromised individuals and patients with cancer. Spontaneous abortion is the loss of a fetus before the 20th week of pregnancy, when occurring naturally without any surgical or phar¬maceutical intervention. Based on previous studies, it appears that L. monocytogenes incidence is high among pregnant women. It can be concluded that, pregnant women and their health care providers should be informed about listeriosis during pregnancy. Manuscript profile

List of Articles Listeria monocytogenes

Sanad

Links

Related Centers

Technical Support

Official pages