Evaluation of the chemical composition,antimicrobial and antioxidant of Potentilla recta extracts

Subject Areas : Food Microbial Contamination

hojjat azad ¹ , Tooraj Mehdizadeh ²

1 - دانشجوی کارشناسی ارشد میکروبیولوژی،گروه زیست‌شناسی،دانشکده علوم پایه،واحد ارومیه،دانشگاه آزاد اسلامی،ارومیه،ایران
2 - گروه بهداشت و کنترل کیفی مواد غذایی دانشکده دامپزشکی دانشگاه ارومیه

Received: 2017-04-20 Accepted : 2017-06-22 Published : 2017-08-23

Keywords: Staphylococcus aureus, Antioxidant, Escherichia coli, Antibacterial, Listeria monocytogenes, Potentilla recta,

Abstract :

The use of herbs has long been common in many societies. The importance of medicinal plants, with the intensification of the therapeutic effect, is that in many cases it can also avoid the toxicity and adverse effects of medication. In this study Potentilla recta, one of the Iran and Azerbaijan region-native medicinal plants, was studied in terms of chemical composition, antimicrobial and antioxidant effect. After collecting, drying and milling of plants, the methanol, ethanol and ethyl acetate extracts obtained by maceration method. To identify the constituents of the extracts, gas chromatography connected tomass spectrometry was conducted. The DPPH radical scavenging method used to measure the antioxidant potential and the results was reported based on the IC50 index. After determine MIC and MBC using broth micro dilution method, agar well diffusion assay conducted to assess the antimicrobial effect of extracts. Data were analyzed by one-way analysis of variance (ANOVA) and means were compared using Duncan test at PL.monocytogenes microbial growth inhibition zone area (p <0.05). All prepared extracts had the ability to scavenging radical 1, 1-Diphenyl-2-picrylhydrazyl and the highest antioxidant potency after 30 minutes related to methanol extract. Most chemical compounds in methanol, ethanol and ethylacetate extracts, were respectively: methyllinoleate and ethyllinoleate. This study showed that the methanol extract of Potentilla recta has great antibacterial and antioxidant properties and with more research can be used in various applications.