Study of two human infectious bacteria in the kapoors(common carp) intestine in province khorasan razavi

Subject Areas : Journal of Animal Biology

امیر  صفاری ¹ (دانشجوی دکترای دامپزشکی و عضو باشگاه پژوهشگران جوان دانشگاه آزاد اسلامی واحد گرمسار)
محمدحسین  غفوریان ² (دانشجوی دکترای دامپزشکی و عضو باشگاه پژوهشگران جوان دانشگاه آزاد اسلامی واحد گرمسار)

Received: 2014-06-23 Accepted : 2017-05-21 Published : 2009-10-23

Keywords: Khorasan Razavi, Listeria monocytogenes, E.coli, cyprinus carpoie,

Abstract :

Incidence of food andndash;borne diseases caused by marine product has been increased in recent years. the aim of this study is to evaluate the late of contamination to listeria monocytogenes and E.coli o157H7. In fishes of Khorasan Razavibefore marketing.Forty fish samples were taken from 10hatchery then alimentary tract of fishes were cultured in related medias. Isolation of listeria monocytogenes was done by cold- Enrichment method for seven days in 4oc in L.E.B. and suspected colonies transferred in to oxford agar .first identification of this bacteria was done by morphological and biochemical rests. Among suspiuons colonies, 5% were positive for listeria monocytogenes. Isolation of E. coli O157H7 in this regard first the digestion of the fisher were taken out in aseptic condition and after mixing that with brian heart infusion broth wich is a general rich environment after 24 hours selected rich environment of E.Coli , M.Cbroth ,being added , after growth of colony related to E.Coli , from E.M.B typic colony ,peptone water ,B.G broth , T.S.I being added. among suspicions colonies 75% rate of colony suspicious samples of E. coli infection were positive Gypsy and none to serotype O157H7 observe . These rates of contamination might refer to high pollution of pisiculture pools and low temperaturein Khorasan Razavi province