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      • Open Access Article

        1 - Study on the overview on food borne bacteria in foodstuffs with animal origin in Iran; Part two: meat and meat products
        S.S Shekarforoush نوردهر Rokni گیتی Karim S.M Razavi Rohani S.M.M Kiaie مریم Abbasvali
           The present study was aimed to review the contamination of meat and meat products with pathogenic bacteria in Iran. Little information is available about the contamination of meat and meat products with Bacillus cereus, because of the low contamination rate More
           The present study was aimed to review the contamination of meat and meat products with pathogenic bacteria in Iran. Little information is available about the contamination of meat and meat products with Bacillus cereus, because of the low contamination rate with the microorganism. The situation is about the same with Brucella, as the microorganism can hardly be seen in the muscles. However, not too many information is available on the contamination with Campylobacter jejuni, even though, the contamination of chicken meat with the campylobacter was addressed. Clostridium butolinum was the main discriminated bacterium to contaminate fish and its products and canned meats. Studies revealed that the big portions of meat were less likely contaminated with E. coli compared to the minced meat. Our study showed that the cross contamination of sausage is often occurred after the production chain. The situation was possibly attributed to the improvement of hygienic conditions of slaughter-houses and meat industries. Limited information was found on the contamination with Listeria monocytogenes and available data indicate that the microorganism can be present in meat and meat products. The most important factor for prevention of contamination is restricted inspection of slaughtered animals before slaughter and omission of diseased animals. Much information was accessible on the cross contamination with Salmonella in Iran and elsewhere. Salmonellosis is not common in the slaughtered animals despite its epidemiological and public health issues. The problem was also associated with the restricted inspection. Study about the staphylococcal contamination of meat was proportionately numerous. The contamination was mostly occurred in the minced Kebab in the warm seasons of the year. Generally, a low percentage of such contaminations were found in the meat products and sausage, in the surveys. Manuscript profile
      • Open Access Article

        2 - Study on the overview on foodborne bacteria in foodstuffs with animal origin in Iran; Part one: milk and dairy products
        S.S Shekarforoush گیتی Karim S.M Razavi Rohani S.M.M Kiaie نوردهر Rokni مریم Abbasvali
           In this study contamination of milk and dairy products with pathogenic bacteria in Iran has been reviewed. There are many surveys in some cases, while there is no information about some pathogenic bacteria in milk or very few data is available. It should be More
           In this study contamination of milk and dairy products with pathogenic bacteria in Iran has been reviewed. There are many surveys in some cases, while there is no information about some pathogenic bacteria in milk or very few data is available. It should be point out that the results come from the cross sectional surveys are needed for further epidemiological and experimental studies. In general the contamination of milk and dairy products has decreased in the course of time as the production methods and producer’s knowledge increased. On the other hand application of new and precise methods of detection and investigation of bacteria lead to excess detection of pathogenic microorganisms in milk. Therefore regular continuation of cross sectional studies along with using   precise and sensitive methods of sampling and analysis of bacteria are necessitated. Besides making available of needed data about the contamination of milk and milk products with pathogenic bacteria, this information make enable to adopt the effective preventive precautions for elimination or reduction of contamination. Manuscript profile
      • Open Access Article

        3 - Investigation of antibacterial effects of ethanolic extract of Sumac (Rhus coriaria L.) against Escherichia coli in vitro
        حمدالله Moshtaghi مریم Abbasvali الناز Mohammadi A.R Safian میلاد Adel
           The antibacterial effect of etbhanolic extract of Sumac (Rhus coriaria L.) was investigated quantitatively and qualitatively on Escherichia coli. The results of well diffusion test showed that extracts of Sumac in concentration of 0.5%, 1%, 2.5% and 5% coul More
           The antibacterial effect of etbhanolic extract of Sumac (Rhus coriaria L.) was investigated quantitatively and qualitatively on Escherichia coli. The results of well diffusion test showed that extracts of Sumac in concentration of 0.5%, 1%, 2.5% and 5% could inhibited E. coli. In this study it was shown that MIC of the alcoholic extract of Sumac against E. coli was 6.25 mg/ml and its MBC against this bacterium was 50 mg/ml. The results from evaluation of the antibacterial effects of the Sumac revealed that at 4 and 15 °C, the growth of E. coli in test tubes containing meat extracts has increased Throughout the 48 h of incubation period. Results showed that the growth of this bacteria in different concentration of Sumac extract as decreased in the both tested temperatures in comparison to time zero (p<0.05). Furthermore, there was a significant difference in the number of microorganism at various times between control and experimental groups in both tested temperatures (p<0.05).  Manuscript profile
      • Open Access Article

        4 - Study on the overview on food borne bacteria in food with animal origin in Iran; Part three: seafood
        S.S Shekarforoush S.M Razavi Rohani گیتی karim S.M.M Kiaie نوردهر Rokni مریم Abbasvali
        The current retrospective study was focused on the contamination of seafood in Iran during the years 1999 to 2012. The isolates were Clostridium botulinum, Clostridium perferingense, Escherichia coli, Listeria monocytogenes, Salmonella, Shigella, Staphylococcus aureus a More
        The current retrospective study was focused on the contamination of seafood in Iran during the years 1999 to 2012. The isolates were Clostridium botulinum, Clostridium perferingense, Escherichia coli, Listeria monocytogenes, Salmonella, Shigella, Staphylococcus aureus and Vibrio parahemolyticus. C. botulinum type E was the most prevalent type of bacteria in the fisheries products, responsible for the most cases of botulinal food poisoning. The presence of coliforms and E. coli in the seafood was due to environmental pollution. Additionally, in the processed food, the contamination of potable water and poor handling of the products are the main sources. L. monocytogenes was isolated from fresh, frozen and processed food. The microorganism is able to grow in the refrigeration condition and multiply in the processed food. The temperature of cold smoking (20-30 ºC) cannot stop growing of Listeria. High prevalence of Salmonella in water and fisheries product was mainly because of the low environmental sanitation and various geographical conditions. However, Compost fertilizer and flowing of the swages through the farms were considered as the major source of contamination. S. aureus is not a typical microorganism of the fisheries farms but may contaminate them during the processing or in-appropriate handling of the products. V. Parahemolyticus is a normal habitant organism of the pelagic area. The organism usually find in the warm water and presents in the tropical conditions. Different species of Vibrio may contaminate the salty water in the warm seasons and so contaminate the aquatics farms in these areas. In general, the prevalence of vibriosis was associated with the consumption of semi-cooked food stuff and/or the secondary contamination of the processed ones. Manuscript profile
      • Open Access Article

        5 - Comparison of antibacterial activity of four cultivars of olive (Olea europaea) leaf extract on Bacillus cereus
        مریم Abbasvali محمود Esmaeili Koutamehr حمدالله Moshtaghi M.H Eskandari
        In recent years, plant extracts have been used as antimicrobial agents. One of these extracts is olive leaf extract, which has antimicrobial properties due to its phenolic compounds. In current study the leaf extract of four olive (Olea europaea) cultivars (Shiraz, Zard More
        In recent years, plant extracts have been used as antimicrobial agents. One of these extracts is olive leaf extract, which has antimicrobial properties due to its phenolic compounds. In current study the leaf extract of four olive (Olea europaea) cultivars (Shiraz, Zard, Roghani and Dezfool) was extracted by different solvents (acetone, methanol and ethanol) using microwave-assisted extraction method. Then the antibacterial activity of the extracts was assessed on Bacillus cereus. The antibacterial activity of the extracts was determined using the standard and micro broth-dilution methods. All experiments were carried out in triplicate. Minimum inhibitory concentration (MIC) of eleven extracts was estimated as 20 mg/ml, and only MIC of the methanolic extract of Roghani cultivar was 10 mg/ml. Bacterial growth curve of B. cereus in the presence of olive leaf extracts was drawn after 24 h incubation at 37 °C and the mean percentage of growth inhibition of each extract was measured after 24 hours. The results showed that in comparison with the other cultivars, the methanolic extract of Roghani cultivar with the percentage of growth inhibition of 91.3% and 87.8% (in the concentrations of 10 and 20 mg/ml, respectively), demonstrated stronger antibacterial effect on B. cereus. This study indicated that olive leaf extract from inexpensive agricultural waste might be a valuable bioactive source with antibacterial activity, and seem to be applicable as a safe food additive.  Manuscript profile
      • Open Access Article

        6 - Study on the overview on foodborne bacteria in food with animal origin in Iran; Part four: Poultry and egg
        S.S Shekarforoush S.M.M Kiaie گیتی Karim S.M Razavi Rohani نوردهر Rokni مریم Abbasvali
           In this study, the contamination of poultry meat and eggs to pathogenic bacteria was investigated in Iran, for over three decades. Health assessment of these two products is highly important due to their high level of consumption and consequent health risks More
           In this study, the contamination of poultry meat and eggs to pathogenic bacteria was investigated in Iran, for over three decades. Health assessment of these two products is highly important due to their high level of consumption and consequent health risks of food borne diseases such as salmonellosis. Many reports indicate the presence of majority of pathogenic bacteria such as campylobacter, staphylococci, salmonellas, etc. in the poultry abattoirs across the country. It seems that most of these contaminations had been occurred through cross contamination during slaughtering or other stages of processing. Although many bacterial contaminations have been reported in eggs (e.g., streptococci, staphylococci, or Escherichia coli), most of studies have focused on salmonella as eggs are among the major transmission routes of this bacterium. In several suveys, salmonella has been isolated from the shell of the eggs of native breeds. According to the studies, campylobacter contamination has not been reported. Moreober, occurrence rate with other bacteria species was very low. It seems that due to the high proportions of chicken meat and eggs in the food basket of community, even low levels of contamination could be of great concern. It was concluded that maintaining of hygienic conditions and improving the knowledge of producers in the food production chain could be effective in reducing the overall contamination of pathogenic bacterial. Manuscript profile
      • Open Access Article

        7 - Analysis of chemical properties and determination of two preservatives in industrial Dough consumed in Shiraz
        M. Abbasvali H. Taleb Najafabadi A. Falah Mehrjerdi
        This study was conducted to determine the quantity of sodium benzoate and potassium sorbate in 61 industrial dough samples (16 different brands with a different production date) in Shiraz market. Moreover, chemical characteristics of the samples (pH, SNF, fat and salt c More
        This study was conducted to determine the quantity of sodium benzoate and potassium sorbate in 61 industrial dough samples (16 different brands with a different production date) in Shiraz market. Moreover, chemical characteristics of the samples (pH, SNF, fat and salt content) were determined. The mean concentrations of potassium sorbate and sodium benzoate were 31.84 and 31.83 mg/kg, respectively. Based on Iranian National Standard (No 2453), none of these preservatives are permitted to be used in the dough. According to the results, 73.8% and 85.3% of the samples contained potassium sorbate and sodium benzoate, respectively and consequently inappropriate for consumption. Moreover, pH, salt, fat and SNF contents in 100%, 95.1%, 70.5% and 100% of the samples were found in the acceptable limit, respectively. Among the samples, 11.5% had potassium sorbate and 23% had sodium benzoate, and 62.3% contained both of the preservatives. There was no significant correlation between the concentration of sodium benzoate and the shelf life of the samples. However, there was a significant correlation between the concentration of potassium sorbate and the shelf life (r= 0.37, p= 0.001). Despite the Iranian National Standard legislation, presence of these chemical preservatives in dough could be a public health concern. Manuscript profile