Oxidative stress

Open Access Article

1 - The effect of the different concentrations of aqueous extracts of Origanum vulgaris in subacute damage of oxidative stress caused by cadmium in kidney of rat
Raeeszadeh, M., Mortazavi, P., Khademi, N., Falah, M.M. .

Due to kidney damage cadmium, this study was an attempt to investigate the protective effect of aqueous extracts of marjoram cadmium in kidney. 30 male Wistar rats divided into 5 equal groups randomly: control and treatment groups 1 to 4 (T1-T4), respectively. T1 group More

Due to kidney damage cadmium, this study was an attempt to investigate the protective effect of aqueous extracts of marjoram cadmium in kidney. 30 male Wistar rats divided into 5 equal groups randomly: control and treatment groups 1 to 4 (T1-T4), respectively. T1 group was administrated at 2 mg/kg cadmium chloride intraperitoneal and T2, T3 and T4 groups in addition to cadmium aqueous extract of marjoram at a dose of 125, 250, 500 mg/kg, respectively. On the last day, the animals were weighed and blood samples for serum preparation were isolated. So that the animals were euthanized with overdose of thiopental. The right kidney was used to measure malondialdehyde and the left one was fixed in formalin-buffer 10% to pathologic studies. The results showed that the T1 group had the lowest average body weight. The different weight between the T1 group and control, 250 and 500 mg/kg of extract was significant. The MDA difference between T1 Group, T4, and control group was significant (P=0.041).  The TCA concentration in T4 group was the highest of 1198.91 ± 51.45 and in T1 was the lowest average 796.41 ± 40.76 µmol/ml and the difference was significant (P=0.036). The pathological damage was graded with the degeneration of tubules of kidney, necrosis and accumulation of inflammatory cells. The high to low scoring damage was found in Group T1, T2, T3, C and T4 respectively. According to the findings, 500 mg/kg of O. vulgaris extract can prevent kidney damage caused by cadmium chloride in rats. Manuscript profile

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2 - The effect of turmeric (Curcuma longa) powder on liver function, serum levels of pro-inflammatory cytokines, and antioxidant status biomarkers in male Japanese quails fed a cadmium-contaminated diet
, O Karimi , M.R Mofdi , M. Bitaraf Sani

Environmental pollution by cadmium (Cd) is increasing. The entry of this heavy toxic metal into the food chain is a serious danger to human and animal populations. This study aims to evaluate the effect of turmeric (Curcuma longa) powder (TP) containing 2.96% of total c More

Environmental pollution by cadmium (Cd) is increasing. The entry of this heavy toxic metal into the food chain is a serious danger to human and animal populations. This study aims to evaluate the effect of turmeric (Curcuma longa) powder (TP) containing 2.96% of total curcuminoides (TCM) in modulating the adverse impacts of Cd in Japanese quail. 180 22-day-old male Japanese quails were randomly divided into six groups for 23 days and fed a basal diet (BD) supplemented with 3 gr/kg TP (containing 88.18 mg of TCM), 5 mg/kg TP (containing 148 mg of TCM), 100 mg/kg Cd, 3 mg/kg TP plus 100 mg/kg Cd, and 5 gr/kg TP and 100 mg/kg Cd. The addition of TP to the Cd-contaminated diet reduced serum levels of pro-inflammatory cytokines TNF-αand IL-6, improved total antioxidant capacity, increased SOD and CAT activity, as well as decreasing MDA concentration. The TP supplementation significantly (p<0.05) reduced the harmful effects of Cd on body weight loss, relative liver weight gain, and total protein concentration, besides increasing serum levels of ALT, AST, and ALP enzymes. The findings of this study suggest that TP may reduce the toxic effects of Cd in Japanese quail by improving antioxidant status and reducing the activity of pro-inflammatory cytokines. The addition of 5 g/kg TP to the Cd-contaminated diet results in more antioxidant and anti-inflammatory properties than 3 g/kg TP. Manuscript profile

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3 - Study of the effect of nerolidol on Alzheimer's disease in Wistar rats
پیمان Taheri پریچهره Yaghmaei زهرا Hajebrahimi کاظم Parivar

10.30495/jcp.2022.20567

Alzheimer's disease is the most important cause of dementia in human societies and the elderly population. One of the most important symptoms of this disease is amyloid plaques in the hippocampus. Inflammation and oxidative stress play an important role in the developme More

Alzheimer's disease is the most important cause of dementia in human societies and the elderly population. One of the most important symptoms of this disease is amyloid plaques in the hippocampus. Inflammation and oxidative stress play an important role in the development of Alzheimer's disease. Nerolidol is one of the many metabolites of plants and has antioxidant and anti-inflammatory properties. Therefore, the aim of this study was to evaluate the effect of nerolidol on beta amyloid-induced model of Alzheimer's in rats. 48 male Wistar rats randomly divided into 8 groups including control, sham, streptozotocin induced model of Alzheimer's, Alzheimer's and drug solvent, Alzheimer's and treatment with donepezil, Alzheimer's and treatment with nrolidol dose 50, Alzheimer's and treatment with nrolidol dose 100 and protection groups (pre-treatment with nerolidol before Alzheimer's induction). Rats weight, catalase activity, and amount of amyloid plaque were measured. Histological studies were performed by fluorescent staining. Streptozotocin increased amyloid plaques, and decreased rat's weight and catalase activity. Donepezil and nerolidol, especially at doses of 100 and in the protective group, reduced the symptoms of Alzheimer's disease through weight gain, increased catalase activity, and decreased amyloid plaques. These findings show that nerolidol can be effective in improving Alzheimer's disease and its damage, and may possibly be effective in preventing Alzheimer's in people with a family history of Alzheimer's. Manuscript profile

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4 - The Effect of Blackberry Extract on the Total Antioxidant Capacity and Lipid Peroxidation after Acute Resistance Exercise in Obese Men
Sirvan Atashak Abdollah Niloofari Kamal Azizbaygi

Introduction: Overweight and obesity are major risk factors for cardiovascular morbidity and mortality, that is associated with increased oxidative stress statues. Moreover, despite of many studies that indicated regular exercise training is associated with numerous hea More

Introduction: Overweight and obesity are major risk factors for cardiovascular morbidity and mortality, that is associated with increased oxidative stress statues. Moreover, despite of many studies that indicated regular exercise training is associated with numerous health benefits, it has been shown that acute exercise might give rise to increased oxidative stress. Therefore, natural supplementation might attenuate oxidative stress in exercise. The present study is conducted to assess the effect of blackberry extract on the total antioxidant capacity and lipid peroxidation after acute resistance exercise in obese men.Materials and Methods: In a randomized double-blind design, twenty obese men (BMIS 30) were assigned into one of two placebo (n = 10) and supplement (n = 10) groups. Supplement group received 100 mg blackberry daily for seven days while other group consumed placebo.All subjects underwent resistance exercise that included nine exercises with 60% 1-RM. Venous blood samples to determine plasma malondialdehyde (MDA) as an index to lipid peroxidation and total antioxidant capacity were obtained one week prior to the exercise, immediately before the exercise and 24 hours after the exercise.Results: The results indicated that MDA concentration significantly increased after resistance exercise in placebo group as compared to the supplement group (P<0.05). Moreover, the mean of total antioxidant capacity remained unchanged in both groups in immediately preexercise and 24 h post exercise (P>0.005).Conclusion: The results of this study indicated that acute resistance exercise give rise to increased MDA concentration in obese men. The application of blackberry extract employed as an antioxidant supplement might ameliorate this marker and prevent the oxidative stress damage. Manuscript profile

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5 - The effect of Anthracene on oxidative stress factors in Medicago sativa L.
Maryam Khoshsokhan mozaffar leyla Jafari Elahe Vatankhah

Polycyclic Aromatic Hydrocarbons (PAHs) are one of the tensions that inhibit plant growth and create imbalance between the production of Reactive Oxygen Species and antioxidant defense activities of the plant. Anthracene is the main pollutants in the petrochemical indus More

Polycyclic Aromatic Hydrocarbons (PAHs) are one of the tensions that inhibit plant growth and create imbalance between the production of Reactive Oxygen Species and antioxidant defense activities of the plant. Anthracene is the main pollutants in the petrochemical industry, which is located on the list of toxic pollutants, US Environmental Protection Agency (USEPA). In this study is to evaluate the effect of anthracene on some parameters of oxidative stress in Medicago sativa after 12 days of treatment with anthracene (2, 4 mmol per liter) in hydroponics, shoot and root tissues of the plants were harvested and used to measure biochemical parameters. The activity of superoxide dismutase(SOD), polyphenol oxidase(PPO), glutathione transferase(GST), peroxidase(PO), malondialdehyde and enzyme ascorbate peroxidase(APX) in shoot and root, as well as the activity of chlorophyll b and a the shoot was determined. The results showed that The high activities of SOD, APX, SPO, PPO and GST on the shoot of 4 mM anthracene is significant. Anthracene treatment, decreased MDA levels in shoot significantly. It is also associated with increased concentrations of chlorophyll a and b. Also increases the activity of the enzyme polyphenol oxidase is the result of the production of phenolic compounds in the plant. Manuscript profile

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6 - Arq ajwain protects nonalcoholic fatty liver disease on high-fat diet-induced obese rat
Md Rafiul Haque Md Afroz Ahmad Kamran Ashraf Md Habban Akhter Mohammad Jameel Abuzer Ali Wasim Akhtar Monika Dhaka Kavita Rana

10.30495/tpr.2021.1940654.1221

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7 - Ameliorative nature of flavonoid naringin: A comprehensive review of antitoxic effects
Zorawar Singh Shivam Sharma Adeshwinder Kaur

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8 - Cymbopogon citratus Stapf (DC) extract attenuates gasoline vapour-induced low-triiodothyronine syndrome, oxidative stress and lipid peroxidation in rats
Christopher Edet Ekpenyong Utibe Essiet

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9 - The Protective Effects of Royal Jelly on the Histomorphometric ,Hormonal Alternation and Oxidative Stress in Ofloxacin Treated Male Rat
Ghodrat Ebadi Manas

Inroduction & Objective: Royal jelly is a compound that is produced by the worker honey bees. This compound has antioxidant activity and protective effects against oxidative stress. The aim of this study were to determine protective role of royal jelly on decrease side More

Inroduction & Objective: Royal jelly is a compound that is produced by the worker honey bees. This compound has antioxidant activity and protective effects against oxidative stress. The aim of this study were to determine protective role of royal jelly on decrease side effects of ofloxacin and return reproductive function near normal rang in ofloxacin treated male ratMaterial and Methods:In this experimental work used 32 male albino rats that each has body weight between 160-18o gand accidentally divided to 4 groups: ofloxacin, royal jelly , ofloxacin with royal jelly and control. Each group included 8 rats. Dosage for ofloxacin group was 216 mg/kg.BW ofloxacin and for royal jelly group was 100 mg/kg.BW royal jelly. Royal Jelly with ofloxacin group received 216 mg/kg ofloxacin and100 mg/kg royal jelly. Control group received distilled water at dose of 216 mg/kg.BW. All groups received dose - related daily for 28 days orally. Data analyzed by one-way ANOVA and used Turkey HSD Post-test to compare the significance of difference. Results: After exposure ofloxacin results revealed thatdiameter of seminiferous tubules, thickness of epithelium seminiferous tubules, weight testes,FSH, LH, testosterone, TTM ,TAOCand RI in compared to control significance decline,but distance between seminiferous tubule ,TDI , SI, MDA and NO significanceincreased. All parameters were improved to near normal rang level after exposure royal jelly.Conclusion:This investigation revealed that ofloxacin has negative effect on histomorphometry and reproductive function in mature male rats,but at the same time if you use royal jelly it is protects against ofloxacin and retune to normal rang. Manuscript profile

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10 - Effect of a course of aerobic exercise combined with vitamin C supplementation on leptin receptor and oxidative stress in the heart tissue of female rats with Alzheimer's
Asiye Seyyed Mazieh Fallahiyan Forough Tajalli Seyedeh Ommolbanin Ghasemian

Background & Aim: Antioxidant enzymes serve as the primary defense against the onslaught of various reactive oxygen radicals. Antioxidants mitigate or prevent oxidative stress by interacting with free radicals and active species. The present study aimed to determine the More

Background & Aim: Antioxidant enzymes serve as the primary defense against the onslaught of various reactive oxygen radicals. Antioxidants mitigate or prevent oxidative stress by interacting with free radicals and active species. The present study aimed to determine the effect of a course of aerobic exercise comined with vitamin C supplementation on leptin receptor, visceral fat weight, body mass index (BMI), and antioxidant enzymes in the brain tissue of rats with Alzheimer's. Materials & Methods: In this experimental study, 45 elderly female rats were randomly assigned to six groups, including controls with Alzheimer's disease, vitamin C supplementation, aerobic exercise, aerobic exercise + vitamin C, and healthy controls. Leptin, visceral fat weight, and BMI after the intervention, as well as malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) indices, were measured after heart tissue extraction. Results: In the intervention groups, leptin receptor values were significantly higher (P=0.001), while visceral fat weight and BMI were lower than in the healthy control group (P=0.001). After eight weeks of aerobic exercise and vitamin C supplementation, MDA levels decreased significantly in the exercise+supplement group (P<0.05) and values of CAT and SOD were significantly increased compared to other groups (P<0.05). Conclusion: It seems that aerobic exercise combined with vitamin C supplements increases leptin receptor expression and decreases visceral fat weight and BMI in rats with Alzheimer's disease. Moreover, it decreases MDA levels and increases CAT and SOD levels. Manuscript profile

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11 - Evaluation of sperm parameters and oxidative stress level in asthenoteratozoospermia men by N-acetylcysteine treatment
rahil jannatifar kazem parivar Mohammad Hossein Nasr-Esfahani nasim hayati roodbari

Background Infertile men have higher levels of semen reactive oxygen species (ROS) than fertile men. High levels of semen ROS can cause sperm dysfunction, sperm DNA damage and reduced male reproductive potential. This study investigated the effects of supplementation wi More

Background Infertile men have higher levels of semen reactive oxygen species (ROS) than fertile men. High levels of semen ROS can cause sperm dysfunction, sperm DNA damage and reduced male reproductive potential. This study investigated the effects of supplementation with N-acetyl-cysteine (NAC) on sperm parameters; chromatin integrity and level of stress oxidative (ROS) in infertile men.Methods The study was carried out in the unit of ACECR Infertility Research Center, Qom, Iran. The patients consisted of 50 infertile men with asthenoteratozoospermia who received NAC (600 mg/d) orally for 3 months, after which they were compared with pre-treatment status. Sperm parameters according WHO (World Health Organization) guidelines, protamine deficiency (chromomycin A3 staining), sperm DNA damage (TUNEL assay), percentage and intensity of ROS (DCFH-DA staining) were assessed in infertile men.Results After NAC treatment, patients' sperm parameters improved (P Manuscript profile

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12 - The evaluation and comparison of metabolic and oxidative stress status in dairy cows before and after parturition
Maryam Maryam Karimi-Dehkord Forough Mohammadi Majid Gholami-Ahangarn

Inroduction & Objective: In the recent years, due to the role of oxidative stress in the pathogenesis of various diseases, oxidative stress markers have become a useful tool in the prevention and treatment of various diseases. Regarding the importance of dairy cattl More

Inroduction & Objective: In the recent years, due to the role of oxidative stress in the pathogenesis of various diseases, oxidative stress markers have become a useful tool in the prevention and treatment of various diseases. Regarding the importance of dairy cattle transition period and little research on the state of oxidative stress during the transition period, the aim of this study was to investigate the status of oxidative stress during the transition period using Malondialdehyde (MDA), glutathione (GSH) and total antioxidant capacity (GSH) two weeks before and after parturition in Holstein dairy cows in Shahrekord dairy farms. Materials and Methods: 42 dairy were selected from one of the industrial dairy farms in Shahrekord. Blood samples were taken two times (two weeks before and after pregnancy) and was transferred to the laboratory for measurement of Malondialdehyde, glutathione and total antioxidant capacity. T-test was used to compare the mean of each of these parameters before and after pregnancy. Results: The mean serum Malondialdehyde level after parturition in comparison to before parturition increased, although it was not statistically significant (P>0/05). The mean glutathione and total antioxidant capacity of the blood after delivery in comparison with the prepartum were significantly decreased (P<0/05). Also, the levels of superoxide dismutase, catalase and glutathione peroxidase enzymes decreased significantly after delivery compared to before delivery (P <0.05). Conclusion: According to the results of this study, it can be concluded that dairy cows are exposed to oxidative stress during the transition period, although they do not show clinical symptoms. As a result, it is important to pay attention to the use of antioxidants during the transition period. . Manuscript profile

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13 - The effect of Endurance Training on Oxidative Stress Induced by Ischemia in Male Rat Heart
ali ojaghi farshad ghazalian Tohid Vahdatpour Hossein Abednatanzi Reza badalzadeh

Inroduction & Objective: Regular exercise reduces the incidence of cardiovascular diseases. In this context, choosing the type and intensity of training as a suitable model for further effectiveness is essential. The aim of this study was to investigate the effect o More

Inroduction & Objective: Regular exercise reduces the incidence of cardiovascular diseases. In this context, choosing the type and intensity of training as a suitable model for further effectiveness is essential. The aim of this study was to investigate the effect of 8 weeks of endurance training on oxidative stress induced by ischemia in male rats.Materials and Methods: In this study, thirty two male Wistar rats in the weight range of 220± 20g were used. After one week of adaptation to the new environment, mice were randomly divided into four groups (n=8): 1- Control group, 2- Isoproterenol group (ISO), 3- Endurance exercise group (EnEx) 4- Endurance exercise group + Isoproterenol (EnEx + ISO). EnEx and EnEx + ISO groups were undergoing endurance training for 8 weeks. At the end of the eighth week of training, Isoproterenol (85 mg / kg) was administered intraperitoneally for 2 consecutive days. Then, the activities of superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT) and malondialdehyde (MDA) were measured with special kits. Results: Results showed that cardiac ischemia significantly decreased antioxidant enzymes and increased lipid peroxidation (MDA) as compared to control and exercise groups, while eight weeks of endurance training before ischemia increased antioxidant enzymes activity and decreased the level of MDA in comparison to ischemia group.Conclusion: The results of this study showed that endurance training can have a therapeutic effect against ischemia-related injuries by reducing oxidative stress and diminish ischemic heart disorders. Manuscript profile

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14 - Effects of Aerobic Exercise and Ethanolic Extract of Purslane Seed on Markers of Oxidative Stress and DNA Damage in lung tissue of Rats Poisoned with Hydrogen Peroxide
Shiva Bahram vash shams parvin farzanegi Mohammad Ali Azarbayjani

Background and aims: The lung is one of the important tissues of the body and is constantly in contact with the highest oxygen pressure and air pollution, which is very sensitive to the production of free radicals due to the low capacity of antioxidant enzymes. The aim More

Background and aims: The lung is one of the important tissues of the body and is constantly in contact with the highest oxygen pressure and air pollution, which is very sensitive to the production of free radicals due to the low capacity of antioxidant enzymes. The aim of the present study was to evaluateeffects of aerobic exercise and ethanolic extract of purslane seed on ATP, O-6-MethylguanineDNA methyltransferase (MGMT), malondialdehyde (MDA) and prooxidant-antioxidant balance(PAB) levels in the lung tissue of rats poisoned with hydrogen peroxide. Materials & Methods: In this experimental trial, 72 male Wistar rats were randomly divided into nine groups: (1) control + H2O2 , (2) aerobic exercise, (3) aerobic exercise and 50 mg/kg purslane seed extract, (4) aerobic exercise and 200 mg/kg purslane seed extract, (5) aerobic exercise and 400 mg/kg purslane seed extract, (6) 50 mg/kg purslane seed extract, (7) 200 mg/kg purslane seed extract, (8) 400 mg/kg purslane seed extract, and (9) healthy control. Oxidative stress was induced by intraperitoneal injection of 1 mmol/kg hydrogen peroxide three times a week for eight weeks. Aerobic exercise was performed three sessions a week for eight weeks, and the purslane seed extract was intraperitoneally injected daily at the mentioned doses.Results: Aerobic exercise and purslane seed extract alone or combined significantly increased ATP, MGMT and significantly reduced MDA and PAB levels in lung tissue of rats exposed to hydrogen peroxide (P<0.05). Moreover, the effect of purslane seed extract was dose dependent. Conclusion: It seems that aerobic exercise together with consumption of purslane seeds each alone and in combination have interactive effects in reducing oxidative stress, repairing DNA and improving mitochondrial function in the lung tissue of rats poisoned with H2O2. Manuscript profile

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15 - Investigating the effect of Eclipta prostrata hydroalcoholic extract on motor activity, avoidance memory and oxidative stress in an animal model of Parkinson's disease in adult male rats
shahrbano alamirostami Maryam Rafieirad

Background and Aims: Parkinson's disease is associated with motor and cognitive disorders, so we investigated the administration of Eclipta prostrata hydroalcoholic extract on learning, memory, motor activity and oxidative stress in Parkinson's model.Materials and metho More

Background and Aims: Parkinson's disease is associated with motor and cognitive disorders, so we investigated the administration of Eclipta prostrata hydroalcoholic extract on learning, memory, motor activity and oxidative stress in Parkinson's model.Materials and methods: 50 adult Wistar rats were divided into five groups: control group, parkinsonian group and three groups treated with eucalyptus perstrata extract in three different concentrations of 50, 100 and 200 mg/kg. Induction of Parkinson's model was done by injecting 6-hydroxydopamine (6-OHDA). One day after the last gavage, motor tests were done. The shuttle box test was used to evaluate learning and avoidance memory. Oxidative stress was evaluated by malondialdehyde, glutathione peroxidase and thiol content.Results: 7 days after the lesion in the MFB, after the administration of apomorphine, the rats turned 360 degrees in the right direction at a rate of more than 10 revolutions per minute. In the movement tests of the parkinsonian group, maintaining balance in rotarod (p<0.001), catalepsy (p<0.001), muscle stiffness (p<0.001), stride length (p<0.001) and avoidance memory (p<0.001) showed a significant difference to the control group. Also, Eclipta prostrata extract significantly improved all kinds of movement disorders caused by Parkinson's disease, and in doses of 50, 100, and 200, it improved memory in Parkinsonian rats (p<0.001). Also, the extract significantly increased the amount of thiol (p<0.001). and glutathione peroxidase (p<0.001) and decreased MDA in hippocampus and striatum tissue (p<0.001).Conclusion: In general, we showed that the hydroalcoholic extract of Eclipta prostrata administered in the animal model of Parkinson's has a favorable effect on memory, learning, motor activity and oxidative stress of the brain. Manuscript profile

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16 - Effect of regular exercise with different intensities on oxidized LDL levels in obese men
Mohadeseh Nematollahzadeh mahani

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17 - Spark Motor Program reduced oxidative stress in boys with Down syndrome
Atefeh Jan Mohammadi Mehrzad Moghadasi

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18 - The effect of selenium nanoparticles on DNA oxidative damage, oxidative stress parameters and ovarian tissue structure in an animal model of polycystic ovary syndrome
Mohammad Amin Edalatmanesh Samaneh Rafiei

Polycystic ovary syndrome (PCOS) is associated with hormonal-metabolic disorders, oxidative stress and ovulation disorders. The present study evaluated the effect of selenium nanoparticles (SeNPs) on the activity of antioxidant enzymes and ovarian tissue structure and o More

Polycystic ovary syndrome (PCOS) is associated with hormonal-metabolic disorders, oxidative stress and ovulation disorders. The present study evaluated the effect of selenium nanoparticles (SeNPs) on the activity of antioxidant enzymes and ovarian tissue structure and oxidative DNA damage in the polycystic ovary syndrome (PCOS) model. 32 female Wistar rats were divided into 4 control groups, PCOS, PCOS+SeNPs0.1 and PCOS+SeNPs0.1. Polycystic ovary syndrome was induced by a single intramuscular injection of estradiol valerate (4 mg/kg) and SeNPs with doses of 0.1 and 0.2 mg/kg were administered orally for 14 days. At the end, the tissue level of superoxide dismutase (SOD), catalase (CAT) enzymes, content of malondialdehyde (MDA) and 8-hydroxydeoxyguanosine (8-OHdG) in the ovarian tissue was measured by ELISA technique and the ovarian tissue structure was examined histopathologically. A significant decrease in the number of follicular cysts, 8-OHdG and MDA levels along with a significant increase in tissue levels of SOD and CAT enzymes were seen in the SeNPs treatment groups compared to the PCOS group. In fact, SeNPs with antioxidant function improved ovarian tissue structure in polycystic ovary syndrome model. Manuscript profile

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19 - A review of oxidative stress in diabetic patients with the approach of exercise training
Pouria Ahmadi Mehran Ghahramani

10.30495/pah.2023.1988357.1043

Introduction: The consequence of the oxidative stress process is the overproduction of free radicals, which are toxic products of oxygen metabolism and cause damage to different part of the cell, such as DNA and membrane and play a major role in the pathogenesis of diab More

Introduction: The consequence of the oxidative stress process is the overproduction of free radicals, which are toxic products of oxygen metabolism and cause damage to different part of the cell, such as DNA and membrane and play a major role in the pathogenesis of diabetes. This article examines information about the role of oxidative stress in diabetic patients and the effect of exercise training on this process in this type of diseases. Methods: To carry out the present research, from the keywords found in more than 43 valid researches in various specialized sites, such as: Google scholar, Science Direct and articles in the journals: Experimental diabetes research, Med Sci Monit, MDPI which were previously in this field such as oxidative stress, ROS and…were done. Findings: The production of reactive oxygen species and lipid peroxidation increases in diabetic patients, the studies in this research show that exercise training can increase the activity of antioxidant systems. Exercise helps to restraint the effects of diabetes in cell damage. Conclusion: Exercise training cause to control oxidative stress in patients with diabetes and contrast with over levels of its. Manuscript profile

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20 - The effect of incremental aerobic exercise after injection of bone marrow-derived stem cells on some indicators of oxidative stress and inflammatory of liver tissue in rats model of fatty liver
hamed Namdar Seyed Abdollah Hashemvarzi Abdorreza Jafari Chashmi

10.30495/nssem.2024.2001663.1018

Introduction: Non-alcoholic fatty liver disease is one of the most common liver diseases of this century, which is associated with changes in inflammatory cytokines and increased oxidative stress.The purpose of this research was to investigate the effect of an increasin More

Introduction: Non-alcoholic fatty liver disease is one of the most common liver diseases of this century, which is associated with changes in inflammatory cytokines and increased oxidative stress.The purpose of this research was to investigate the effect of an increasing aerobic exercise period after bone marrow stem cell injection on some indicators of oxidative and inflammatory stress of liver tissue in rats model of fatty liver. Methods: 60 adult male Wistar rats were divided into two main groups: control (6 rats) and patients with fatty liver (54 rats). The control group used standard rodent food for 6 weeks, while the animals in the diseased groups used high-fat food to induce fatty liver disease. At the end of the sixth week, to confirm the development of fatty liver disease, blood was randomly drawn from the tails of 3 mice and ALT and AST enzymes were measured. Then the animals of the patient group were randomly divided into 5 groups: Saline, Fatty liver, Exercise, Cell, Exercise + Cell. About 105 cells in 2 microliters of medium were injected into the cell receiving groups for each rat through the tail vein. The training program consisted of 8 weeks of incremental treadmill running.Results: The levels of CAT and SOD increased in the treated groups, but the level of the inflammatory index TNF-α decreased, but these changes were significant only in the groups of exercise and exercise + cells compared to the fatty liver group.Conclusion: Incremental aerobic training after the injection of bone marrow-derived stem cells increased the antioxidant levels of CAT and SOD and decreased the inflammatory index of TNF-α in mice with fatty liver. Therefore, it seems that this method can improve liver condition and be considered as a therapeutic solution. Manuscript profile

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21 - Study on the effects of ethanolic extract of broccoli on oxidative stress induced by acetaminophen in rat kidney
Hossein Jeddi داریوش مهاجری

Acetaminophen overdose causes kidney injury. The aim of the present study was to evaluate the protective effect of ethanolic extract of Brassica oleracea L. var. italica against acetaminophen -induced damage in rat kidney. Male Wistar rats were randomly assigned into 4 More

Acetaminophen overdose causes kidney injury. The aim of the present study was to evaluate the protective effect of ethanolic extract of Brassica oleracea L. var. italica against acetaminophen -induced damage in rat kidney. Male Wistar rats were randomly assigned into 4 groups of 8 animals each. Groups I (control) and II received intraperitoneal injections of normal saline (10 ml/kg) and ethanolic extract of broccoli (200 mg/kg) every other day for 14 days. Group Ш received a single intraperitoneal injection of acetaminophen (750 mg/kg). Group IV received ethanolic extract of broccoli similar to group Ш after administration of a single intraperitoneal injection of acetaminophen (750 mg/kg). At the end of experiment, serum levels of urea, uric acid and creatinine were assessed. Moreover, histopathological observations were also conducted to assess the degree of renal tissue injury. Product of lipid peroxidation (malondialdehyde) and activities of superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase were assayed in kidney homogenates. In acetaminophen-treated rats, ethanolic extract of broccoli significantly decreased serum urea, uric acid and creatinine and renal tissue peroxidation levels and elevated the values of anti-oxidants. Histopathologically, ethanolic extract of broccoli ameliorated acetaminophen-induced kidney injury. The results of this study indicated that ethanolic extract of broccoli exerts a protective effect against acetaminophen induced renotoxicity in rats which might be related to its anti-oxidant potential. Manuscript profile

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22 - The status of oxidative stress and some trace elements, hematological and serum biochemical indices in dairy cows with left displacement of the abomasum
Kambiz Kiani Monfared Gholam Reza Mohammadi Seyed alireza Taghavi razavizadeh Mohammad Heidarpour

Left displacement of the abomasum (LDA) is one of the most important production disorders. In order to compare the status of some hematological and serum biochemical indices, trace elements and oxidative stress of cows with and without LDA, blood samples were taken from More

Left displacement of the abomasum (LDA) is one of the most important production disorders. In order to compare the status of some hematological and serum biochemical indices, trace elements and oxidative stress of cows with and without LDA, blood samples were taken from 30 dairy cows with LDA and 30 healthy cows in early lactation period. Haematological parameters, total protein, fibrinogen, Ca, Mg, bilirubin, uric acid, albumin, cholesterol, triglyceride, urea, creatinine and AST (Aspartate aminotransferase), ALP (Alkaline phosphatase), GGT (Gamma- glutamyl transferase), CPK (Creatine phosphokinase) enzymes, Fe, Zn, Cu, Se, MDA and total antioxidant status were measured. Although the results showed significant differences in PCV, RBC, MCHC, lymphocyte, monocyte, fibrinogen, bilirubin, albumin, cholesterol, calcium, uric acid, AST and ALP enzymes between the two groups (p<0.05), but the occurrence of oxidative stress and changes in the levels of trace elements were not significant between the LDA affected and healthy cows. In LDA affected cattle, significant increase of PCV might be due to dehydration while increasing of monocyte and decreasing of lymphocyte counts could also be due to stressful condition or probable infections in early lactation. Anorexia, food deprivation and withdrawal of calcium from milk in early lactation may play a role in decreasing of albumin, cholesterol and calcium. Liver lipidosis and fasting along with biliary duct obstruction may be effective on increasing AST activity and bilirubin respectively in cows with LDA. It seems that early diagnosis and treatment of LDA can prevent the occurrence of sever changes in oxidative stress status and homeostasis of cow’s body. Manuscript profile

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23 - The association of some blood metabolites and NF-κB gene expression with subclinical ketosis in Holstein dairy cows during the transition period
Saeideh Moradi Gholamali moghaddam Samad lotfollahzade Raziallah Jafari Abbas Rafat

10.30495/jvcp.2022.1937013.1316

Subclinical ketosis is one of the most important metabolic diseases which irreversible economic effects on the dairy industry. Subclinical ketosis has been associated with increases reactive oxygen species and oxidative stress in cells. Since the nuclear factor kappa-B More

Subclinical ketosis is one of the most important metabolic diseases which irreversible economic effects on the dairy industry. Subclinical ketosis has been associated with increases reactive oxygen species and oxidative stress in cells. Since the nuclear factor kappa-B is known as a fast cellular response factor to endogenous stress; this study was conducted to evaluate relative gene expression and some blood metabolites in dairy cattle during the transition period were performed. Blood samples (5 mL) were obtained from the tail vein of 100 cows with fourth and fifth lactation one week pre-parturition and one week post-parturition. In the present study, It was found positive correlations between plasma NEFA concentrations in the pre-parturition period and BHBA, GGT, MDA, and NF-κB and negative correlations between plasma NEFA concentration and HDL. As well as, the positive correlations between BHBA plasma concentration and NEFA , ALP, GGT, MDA and NF-κB and negative correlations between plasma BHBA concentration and HDL in the post-parturition period. Also while the cut-off point BHBA≤1.2(Health) and BHBA≥ 1.2(mmol/l)(subclinical ketosis or disease) in the post-parturition period was designated, it was observed that NEFA(p<.0001), BHBA(p<.0001), MDA(p<.0001), GGT(p<.0001) and ALP(p=0.03) were gradually increased, and HDL(p= <.0001) gradually decreased in disease group. The results showed that the activity of NF-κB (p<.0001) was gradually increased in the disease group. Also results showed that increased NEFAs and BHBA increased inflammation response through activating the NF-κB pathway and oxidative stress. Manuscript profile

Open Access Article

24 - Effect of astaxanthin on testicular damage induced by cadmium chloride in adult male Wistar rats
Mahvash Hafez-Amini Akram Eidi Pejman Mortazavi Fattah Sotoodehnejadnematalahi

10.30495/jvcp.2022.1942072.1330

The protective role of astaxanthin on human health, including the prevention of cardiovascular disease, prevention of cell aging, refers to the very strong antioxidant properties of this substance. Cadmium is one of the pollutants in ecosystems. This heavy metal acts as More

The protective role of astaxanthin on human health, including the prevention of cardiovascular disease, prevention of cell aging, refers to the very strong antioxidant properties of this substance. Cadmium is one of the pollutants in ecosystems. This heavy metal acts as a toxin on the reproductive system and destroys the process of spermatogenesis in testicular tissue. The aim of this study was to evaluate the effect of astaxanthin on cadmium chloride induced testicular damage in adult male Wistar rats. In this study, 45 rats were randomly divided into 9 groups. The healthy control group did not receive any treatment. Control group, physiological serum, infertile control group, cadmium chloride (1 mg / kg body weight) by intraperitoneal injection, astaxanthin receiving groups (5, 10 and 20 mg / kg body weight) by gavage and groups They received cadmium chloride and astaxanthin (5, 10 and 20 mg / kg) at the same time. Test samples from testicular tissue homogenates were used to measure oxidative stress parameters. The parameters were analyzed by photometric method with ELISA reader and microplate reader. The results showed that astaxanthin (10 and 20 mg / kg body weight) increased the antioxidant enzymes SOD, GPX and CAT and astaxanthin (10 mg / kg body weight) decreased MDA. Thus, astaxanthin may act as an antioxidant in protecting the rat testis against oxidative stress induced by cadmium chloride. Manuscript profile

Open Access Article

25 - Assessment of oxidative stress indexes and BCS in clinical mastitis cows in comparison with healthy cows
Maryam Karimi Dehkordi Seyedeh Ommolbanin Ghasemian

10.30495/jvcp.2022.1906232.1277

Mastitis, as one of the most important diseases in cattle, is the cause of the greatest economic loss in the dairy industry. The occurrence of mastitis is associated with the development of immune responses and an increase in the level of reactive oxygen species (ROS). More

Mastitis, as one of the most important diseases in cattle, is the cause of the greatest economic loss in the dairy industry. The occurrence of mastitis is associated with the development of immune responses and an increase in the level of reactive oxygen species (ROS). The production of high amounts of ROS and the lack of optimal amounts of antioxidant compounds are associated with oxidative stress. The aim of this study was to evaluate the status of oxidative stress indices in cows with clinical mastitis. In this study, two groups of cows were studied: healthy cows and cows with clinical mastitis. The body condition score (BCS) of the cows was assessed at the time of blood sampling.Total antioxidant capacity and selenium concentration were significantly higher in healthy cows than sick cows. Healthy cows had lower malondialdehyde concentrations than sick cows, although this difference was not statistically significant. When the cows were divided into three groups according to body score, the amount of glutathione peroxidase in cows with high body scores was significantly lower than the other two groups. The amount of malondialdehyde and the number of milk somatic cells in cows with high and moderate body scores were significantly higher than the other group.The results show that there are changes in the antioxidant defense of cows with mastitis that lead to oxidative damage, so the use of antioxidants is necessary to control mastitis. Obese cows are also more sensitive to oxidative stress. Manuscript profile

Open Access Article

26 - Protective effect of coenzyme Q10 supplementation on the oxidative stress and histological injury caused by nicotine in the ovary of rats
Shahnaz Yousefizadeh Ali Louei monfared Yahya Mohammadi

10.30495/jvcp.2023.1978256.1393

As a poison, nicotine leaves irreparable effects on various tissues of the body, including the reproductive system. Coenzyme Q10 has a tremendous effect in raising the body's antioxidant level and preventing poisoning. In the current study, the protective effect of coen More

As a poison, nicotine leaves irreparable effects on various tissues of the body, including the reproductive system. Coenzyme Q10 has a tremendous effect in raising the body's antioxidant level and preventing poisoning. In the current study, the protective effect of coenzyme Q10 supplementation on the changes induced by nicotine in the rat ovary was investigated. For this purpose, 32 adult female Wistar rats were randomly divided into 4 groups of 8. The rats of the first group (control) were only fed with the usual diet, but the animals of the second group were treated with nicotine at the rate of 0.2 mg/kg, the rats of the third group were treated with coenzyme Q10 at the rate of 75 mg/kg and the animals of the fourth group were treated simultaneously using nicotine and coenzyme Q10 (with the same amounts) through gavage. At the end of the study period (45 days), the animals were euthanised and blood samples were taken for hormonal and antioxidant tests. Also, ovarian tissue was sampled for histological studies. In animals treated with nicotine, there was an increase in follicular atresia, tissue damage, an increase in malondialdehyde and nitric oxide, along with a decrease in the number of ovarian follicles, a decrease in the amount of estrogen and follicle stimulating hormones, and the activity of superoxide dismutase, glutathione peroxidase and catalase. But in animals receiving nicotine and coenzyme Q10 at the same time, there was a significant improvement in ovarian function and structure. This study shows coenzyme Q10 can play a protective role against oxidative stress and tissue changes caused by nicotine in the ovaries of rats with the mechanism of balancing the antioxidant status and reproductive hormones. Manuscript profile

Open Access Article

27 - Comparison of oxidative stress between Cytauxzoon feli infected and healthy cats
Kimia Zafari Seyedeh Ommolbanin Ghasemian

10.30495/jvcp.2024.1984795.1413

Cytauxzoonosis is an emerging infectious disease that affects feral as well as domestic cats. This disease is caused by apicomplexan protozoan parasites belonging to the genus Cytauxzoon. Evaluation of oxidative stress indicators can clarify the adverse effects of Cytau More

Cytauxzoonosis is an emerging infectious disease that affects feral as well as domestic cats. This disease is caused by apicomplexan protozoan parasites belonging to the genus Cytauxzoon. Evaluation of oxidative stress indicators can clarify the adverse effects of Cytauxzoon¬ felis parasite on domestic cats. The present study aimed to investigate the prevalence of C. ¬felis parasite in cats of Shahrekord, Iran and to evaluate oxidative stress indicators in cats infected with C. ¬felis and compare it with healthy cats. This experimental study was conducted on blood samples of 100 cats that were referred to Shahrekord Veterinary Laboratory. Blood samples were taken in two separate test tubes for molecular analysis and determination of antioxidant parameters including catalase and lipid peroxidation. After collecting the samples, the PCR test was performed and oxidative stress markers were measured. The results of the present study showed that 3% of examined cats were infected with C. ¬felis, and the level of catalase enzyme in the cats infected with C. ¬felis was reduced by 9% compared to the healthy group, which was statistically significant (p=0.004). Moreover, lipid peroxidation level was significantly increased in cats infected with C.¬ felis compared to the parasite-free group (p=0.021). The findings of the present study showed that the prevalence of C.¬ felis parasite in cats was 3%. A decrease in the level of catalase enzyme and an increase in lipid peroxidation were observed in cats infected with C.¬ felis compared to cats free of the parasite. Manuscript profile

Open Access Article

28 - Comparative effect of different concentrations of hydro-ethanolic extract of chamomile on freeze-thawn semen quality of rams
hossein daghighkia فاطمه Sadeghi Sadegh Abad مرضیه Ebrahimi فرهاد Samadian

Oxidative stress during freeze-thawing process causes reduction in motility, viability, membrane functions and finally sperm fertility. The aim of this study was to determine the effect of Chamomile extract as natural antioxidant on quality of cryopreserved ram sperm. I More

Oxidative stress during freeze-thawing process causes reduction in motility, viability, membrane functions and finally sperm fertility. The aim of this study was to determine the effect of Chamomile extract as natural antioxidant on quality of cryopreserved ram sperm. In this study semen was collected from five mature rams twice a week using an artificial vagina and the ejaculates were pooled equally in order to eliminate the individual effects. Different levels of extract of Chamomile (0, 50, 66.66, 100 and 200 ml/dl of diluent solution) were added to diluent based tris-egg yolk. After cooling, filling and sealing of the samples, they were frozen with nitrogen vapor and immersed in liquid nitrogen and were stored until evaluation time. After thawing, results showed that addition of 66.66 ml/dl of extract increased total motility, progressive motility, curvilinear velocity, straight–line velocity, linearity, viability and plasma membrane integrity of sperm compared to the control and other treatment groups (p<0.05). Also addition of 200 ml/dl extract had significant negative effects on motility parameters, viability and plasma membrane integrity of sperm (p<0.05). Inclusion of different doses of Chamomile extract caused no significant reduction on malondialdehyde concentration in comparison to control but this reduction was significant at 66.66 ml/dl in comparison to 100 and 200 ml/dl of extract. Conflict of interest: None declared. Manuscript profile

Open Access Article

29 - Study on protective effect of Naringenin (Citrus flavonone) on incipient diabetic hepatopathy in alloxan-induced diabetic rats
daryoush mohajeri ghafour mousavi ramin kaffashielahi mehrdad neshatgharamaleki

Abstract    Diabetes mellitus is a metabolic disorder and its incidence is considered to be high all over the world. Hepatic insufficiency is one of the most important consequences in this disease. A multitude of drugs has been described for the treatment of More

Abstract    Diabetes mellitus is a metabolic disorder and its incidence is considered to be high all over the world. Hepatic insufficiency is one of the most important consequences in this disease. A multitude of drugs has been described for the treatment of diabetes throughout the world. The aim of the present study was to assess the protective effect of Naringenin on early liver injury in alloxan-induced diabetic rats. Forty male Wistar rats were randomly assigned into 4 different groups of 10 rats each, including healthy control rats, normal healthy rats receiving Naringenin (50 mg/kg), diabetic rats and diabetic rats receiving Naringenin (50 mg/kg). Diabetes was induced with a single injection of alloxan (120 mg/kg i.p.). Naringenin groups received the drug daily for 3 weeks through gavage. At the end of the experiment, levels of liver function marker enzymes AST (Aspartate aminotransferase), ALT (Alanine aminotransferase) and ALP (Alkaline Phosphatase), TB (Total Bilirubin), Alb (Albumin) and TP (Total Proteins) were assessed in serum. Product of lipid peroxidation (Malondialdehyde; MDA), activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and glutathione reductase (GR) were also assayed in liver homogenate to evaluate antioxidant activity. Moreover, histopathological observations were made to assess the degree of hepatic injury. In alloxanized diabetic rats, Naringenin significantly decreased the levels of serum biomarkers of hepatic injury and TB, and elevated the levels of Alb and TP. Furthermore, Naringenin significantly decreased the lipid peroxidation and elevated the levels of antioxidant enzymes in these rats. Histopathological changes were in agreement with biochemical findings. The findings of this study indicated that Naringenin due to its antioxidant activities protects rats liver from early diabetic hepatopathy. Manuscript profile

Open Access Article

30 - Effects of silver nanoparticles synthesized through chemical reduction on plasma superoxide dismutase and glutathione peroxidase enzymes in rat model
elham ghavidelaghdam mohamad narimanirad alireza lotfi

Abstract    With possibility of synthesis of silver nanoparticles in citrate-coated form and via chemical method, the aim of this study was synthesis of silver nanoparticles by chemical reduction method and investigation of the impact of nanoparticles on super More

Abstract    With possibility of synthesis of silver nanoparticles in citrate-coated form and via chemical method, the aim of this study was synthesis of silver nanoparticles by chemical reduction method and investigation of the impact of nanoparticles on superoxide dismutase and glutathione peroxidase enzymes in an animal model. Silver nanoparticles were synthesized by chemical reduction with a mixture of distilled water and sodium borohydrate and adding silver nitrate and sodium citrate to the obtained solution. In vivo study was conducted using 40 adult male rats with an average weight of 100 grams. Animals were divided into four groups, as control, placebo, and treatment groups receiving silver nanoparticle solutions (100 and 200 mg/kg, respectively). The treatment groups received silver nanoparticle solutions (100 and 200 mg/kg) on the first and the seventh days of experiment intraperitoneally. Oxidative effects of injected high concentrations of silver nanoparticles (200 mg/kg) lead to mortality in the experimental animals. Infusion of silver nanoparticles at concentrations of 100 and 200 milligrams per kilogram of body weight decreased the activity of plasma superoxide dismutase (SOD) and glutathione peroxidase (GPx). This reduction was significantly higher (p<0.01) at the dose of 200 mg/kg. In conclusion, studies on animal models showed that the concentrations of 100 and 200 milligrams per kilogram of body weight of silver nanoparticles synthesized by chemical reduction method decreases the activity of antioxidant enzymes, eventually leading to mortality. Manuscript profile

Open Access Article

31 - The effects of aqueous extract of white tea on serum antioxidant enzymes in rats exposed to arsenic
mohammadhassan rasoulifard felor zargari

Oxidative stress is a condition is which the biological system's ability to detoxify and eliminate harmful effects of free radicals is not sufficient and oxidative damages to cells or tissues  leads to the development of diseases such as cancer, arteriosclerosis an More

Oxidative stress is a condition is which the biological system's ability to detoxify and eliminate harmful effects of free radicals is not sufficient and oxidative damages to cells or tissues  leads to the development of diseases such as cancer, arteriosclerosis and degenerative changes. Phenolic compounds due to their high antioxidant capacity, have an important role in health and increase the antioxidant defense against oxidative stress. The aim of this study was to evaluate the effect of aqueous extract of white tea on status of antioxidant enzymes (SOD, CAT and GPx), MDA (malondialdehyde) and TAC (total antioxidant capacity) in rats treated with sodium arsenite. In this study, 32 adult male rats weighing 200-250 g were used in four groups of eight. The first group included healthy normal rats (control group), the second group of rats were treated with sodium arsenite (100 ppm in drinking water) the third group of rats were treated with aqueous extract of white tea at a concentration of 1/5%, via gavage, the fourth group of rats were treated with aqueous extract of white tea (1/5%) via gavage with sodium arsenite (100 ppm in drinking water). The rats were killed at the end of the 28th day of treatment and blood samples were collected and the antioxidant enzymes of CAT (catalase), SOD (superoxide dismutase), GPx (glutathione peroxidase), and MDA and TAC were measured. The results indicate that the aqueous extract of white tea significantly increased the activities of SOD, GPx, CAT and TAC and decreased   MDA concentration (p<0.05). The results showed consummation of white tea decreased the oxidative stress of arsenic by increasing the activity of antioxidant enzymes and potentiation of antioxidant defense system.   Manuscript profile

Open Access Article

32 - Effect of Spirulina Supplementation and Aerobic Training on Oxidative Stress in Overweight Elderly Men
Mostafa Babaei Ahmad Abdi Asieh Abbassi Daloii Javad Mehrabani

10.30495/varzesh.2022.1953480.1032

Background: Aging and overweight are associated with an increase in oxidative stress indices. The aim of this study was to evaluate the effect of Spirulina supplementation (SP) with aerobic exercise (AT) on oxidative stress in overweight elderly men.Methods: In this cli More

Background: Aging and overweight are associated with an increase in oxidative stress indices. The aim of this study was to evaluate the effect of Spirulina supplementation (SP) with aerobic exercise (AT) on oxidative stress in overweight elderly men.Methods: In this clinical trial study, 40 overweight adult men (age 57.41±4.82 years, Body mass index: BMI 27.88±2.21 kg/m2) were selected from Bandar-e-Anzali and randomly allocated into four groups; including Overweight (OW), Overweight-Aerobic Training (OWAT), Overweight-Spirulina (OWSP) and Overweight-Aerobic Training -Spirulina (OWATSP). The OWSP and OWATSP groups were provided with two 500 mg SP tablets daily in the morning and evening. Training groups participated in an aerobic exercise program for eight weeks, five sessions per week (with an intensity of 65 to 85% of peak heart rate, 40 minutes). Data were analyzed using ANCOVA at a significance level of P<0.05.Results: It was shown that AT and SP increased SOD (P=0.001 and P=0.011), GPX (P=0.0001 and P=0.0001) and CAT (P=0.005 and P=0.018). Simultaneous intervention of AT and SP also had a positive and strengthening effect on increasing SOD (P=0.00001), GPX (P=0.00001) and CAT (P=0.0001). MDA also had a significant decrease in OWAT (P = 0.001), OWSP (P = 0.001) and OWATSP (P = 0.0001) groups.Conclusion: AT and SP could improve the oxidative stress in overweight adult men, by altering the levels of SOD, GPX, CAT and MDA. Nevertheless, the effect of the combination of exercise and supplementation was greater. Manuscript profile

Open Access Article

33 - The Effect of Salvia Extract Supplementation on Inflammation, Oxidative Stress, Muscle Injury, and Pain Following Exhaustive an Eccentric Exercise in non-Athlete Women
zahra koohestani Mohammad-Ali Kohanpour Craig Duncan sara naeimi

10.30495/varzesh.2022.1967784.1042

Introduction: Delayed onset muscle soreness is one of the problems in people starting exercise and researchers are looking for ways to reduce it. The aim of the present study was to investigate the effect of a period of salvia extract supplementation on the indicators o More

Introduction: Delayed onset muscle soreness is one of the problems in people starting exercise and researchers are looking for ways to reduce it. The aim of the present study was to investigate the effect of a period of salvia extract supplementation on the indicators of delayed onset muscle soreness following exhaustive eccentric exercise in non-athlete women.Materials and Methods: 20 non-athlete women aged 20 to 30 years were randomly divided into two groups of placebo and salvia extract. On the pre-test day, both groups participated in an exhaustive eccentric activity. Then, for two weeks, the supplement group received salvia extract, and the placebo group received flour powder (200 mg/kg daily with similar capsules). After two weeks, both groups participated in the same physical activity similar to the pre-test (post-test). Blood samples were taken before and 24 hours after both activities and muscle pain were measured. CK, LDH, GPX, SOD, MDA, TNF-α, IL-1β, CRP, and lactate levels were measured for each blood sample. To examine the changes in the variables, an analysis of variance mixed with repeated measures and an independent t-test at the level of P≤0.05 were used.Findings: The results showed that muscle pain and levels of CK, LDH, GPX, MDA, TNF-α, IL-1β, CRP, and lactate increased significantly after eccentric exhaustion activity, but their increase was significantly lower in the salvia extract group than in the placebo group (P=0.036, P=0.043, P=0.009, P=0.001, P=0.006, P=0.002, P=0.007, P=0.005, and P=0.011, respectively).Conclusion: Short-term salvia extract supplementation may reduce the rate of increase in delayed onset muscle soreness following an exhaustive eccentric exercise due to flavonoids and phenolic compounds. Manuscript profile

Open Access Article

34 - The Effect of Beetroot Supplementation on Oxidative Stress and Delayed Onset Muscle Soreness after an Exhaustive Eccentric Exercise in Competitive Soccer Players
Mohammad-Ali Kohanpour Mohammad Reza Pourbahram Zahra Koohestani Sini Sara Naeimi Mahnaz Seifi

10.30495/varzesh.2023.1979591.1051

Introduction: The aim of the present study was to investigate the effect of beetroot supplementation on oxidative stress and delayed onset muscle soreness after an exhaustive eccentric exercise in competitive soccer players. Materials and Methods: 20 male competitive fo More

Introduction: The aim of the present study was to investigate the effect of beetroot supplementation on oxidative stress and delayed onset muscle soreness after an exhaustive eccentric exercise in competitive soccer players. Materials and Methods: 20 male competitive football players of Mashhad, aged between 17 and 19 years, were randomly assigned to placebo and beetroot groups. On the pre-test day, both groups participated in an exhaustive eccentric exercise. Then, for 2 weeks, the supplement group received beetroot extract and the placebo group received flour powder (200 mg daily dose with identical capsules). After two weeks, both groups again participated in the same physical activity as the pre-test (post-test). Before and 24 hours after both activities, blood samples were taken and muscle pain was measured. CK, LDH, GPX, SOD and MDA levels were measured for each blood sample.Findings: The results showed that muscle pain and levels of CK, LDH, GPX and MDA increased significantly after eccentric exercise, but their increase in the beetroot group was significantly lower than the placebo group (P<0.05).Conclusion: Probably, the short-term supplementation of beet reduces the rate of delayed onset muscle soreness following a bout of eccentric resistance activity due to its antioxidant substances. Manuscript profile

Open Access Article

35 - The Effect of Eight Weeks of High Intensity Interval Training with Citrus Aurantium Extract Consumption on Oxidative Stress and Antioxidant Levels of Soleus Muscle in Elderly Rats
Maryam Kheirdeh Mehdi Noura Rasoul Jamali Fashi Abdolali Rakhshanizadeh

10.30495/varzesh.2020.677910

Abstract Introduction: Aging is a biological process that causes muscle dysfunction and muscle atrophy by increasing oxidative stress. Although the role of exercise and some herbs in improving muscle metabolism has been identified, the effect of high intensity interval More

Abstract Introduction: Aging is a biological process that causes muscle dysfunction and muscle atrophy by increasing oxidative stress. Although the role of exercise and some herbs in improving muscle metabolism has been identified, the effect of high intensity interval training (HIIT) and Citrus aurantium (CA) consumption on oxidative stress in muscle tissue following aging is unknown. Therefore, the present study aimed to investigate the effect of eight weeks of HIIT with CA consumption on glutathione peroxidase (GPx), malondialdehyde (MDA) and carbonyl protein (PC) levels in the soleus muscle tissue of elderly rats. Methods: In this experimental study, 35 elderly rats with the age range of 14 to 18 months, and approximate weight of 270 to 320 g were randomly divided into (1) control (C), (2) sham (Sh), (3) HIIT, (4) CA and (5) HIIT+CA groups. Groups 3 and 5 performed HIIT for eight weeks, five sessions per week (at 85-110% VO2max and speed of 15-25 m/min). Also, groups 4 and 5 received 300 mg/kg/day Citrus aurantium extract peritoneally for eight weeks. To analyze the data, one-way analysis of variance with Tukey's post- hoc test was performed in Graph Pad Prism 8.3.0 software (P≥0.05). Results: HIIT increased GPx and decreased PC (P≥0.05). CA consumption and HIIT and CA interaction increased GPx and decreased MDA and PC in the soleus muscle tissue of elderly rats (P≥ 0.05). Conclusion: It seems that HIIT and CA consumption separately and synergistically have a beneficial effect on reducing oxidative stress and increasing antioxidant activity, however, further studies in this regard seem necessary. Keywords: Aging, Citrus Aurantium, Antioxidant, High Intensity Interval Training (HIIT), Muscle, Oxidative Stress   Extended Abstract Introduction Aging as a biological phenomenon causes muscle atrophy by decreasing mitochondrial function, increasing reactive oxygen/nitrogen species (RONS), decreasing superoxide dismutase (SOD), glutathione peroxidase (GPx), increasing malondialdehyde (MDA) and carbonyl protein (PC) (1). But regular exercise increases the expression of antioxidants in skeletal muscles by increasing mitochondrial biogenesis and protein kinases. High and moderate intensity training also increase SOD, GPx and PC. Aerobic, anaerobic and combined training increase MDA, GPx, SOD, and glutathione reductase (GR) (3, 7, 8) as well. Also, consumption of medicinal plants such as Citrus aurantium (CA) increases mitochondrial biogenesisand sirtuin 1. It reduces inflammatory factors, and has favorable effects on the elderly rats (9, 11). Due to the unknown effect of HIIT and CA consumption on antioxidant enzymes and oxidative stress in muscle tissue, the present study aimed to investigate the effect of eight weeks of HIIT and CA consumption on oxidative stress and antioxidant levels in the soleus muscle of elderly rats.   Methods In this experimental study, 35 elderly rats with the age range of 14 to 18 months, and approximate weight of 270 to 320 g were randomly assigned to (1) control (C), (2) sham (Sh), (3) HIIT, (4) CA and (5) HIIT + CA groups. Groups 3 and 5 performed HIIT for eight weeks, five sessions per week (at 85-110% VO2max and speed of 25-25 m/min) (12). Also, groups 4 and 5 received 300 mg/kg/day Citrus aurantium extract peritoneally for eight weeks (13). 48 hours after the last training session and by using a combination of ketamine and xylazine, the rats were anesthetized and their soleus muscles were extracted by the laboratory experts. To measure MDA levels, MDA measuring kit made by ZellBio GmbH, Ulm, with a sensitivity of 0.1 micromolar was used, and PC surfaces were measured by spectrophotometry using PC measuring kit made by Kiazist Iran company with a sensitivity of nanomoles per milligram. Also, GPx levels were measured using Nagpix ™ Glutathione Peroxidase kit using BMG Labtech Ltd., Aylesbury, UK. One-way analysis of variance with Tukey's post hoc test was used to analyze the results. Data analysis and plotting of research charts were performed using Graph Pad Prism 8.3.0 software (P≥0.05).   Results The mean and standard deviation of GPx, MDA and PC levels in the research groups are presented in Figures 1 to 3. The results of one-way analysis of variance showed a significant difference in GPx (P=0.001), MDA (P=0.001) and PC (P=0.001) levels in the muscle tissue of elderly rats in the research groups. The results of Tukey’s post- hoc test showed that GPx levels in the C and Sh groups were not significantly different (P=0.57), but in the HIIT (P=0.001), CA (P=0.001) and HIIT + CA (P=0.001) groups were significantly higher than the C group. In the HIIT (P=0.001), CA (P=0.001) and HIIT + CA (P=0.001) groups, the levels were significantly higher than the Sh group. No significant difference was observed in the CA and HIIT groups (P=0.99), but in the HIIT+CA group, the levels were significantly higher than the HIIT (P=0.001) and CA (P=0.001) groups (Figure 1). MDA levels in the C and Sh groups were not significantly different (P=0.99), but in the CA (P=0.02) and HIIT+CA (P=0.001) groups, they were significantly lower than the C group; also in the CA (P=0.007) and HIIT+CA (P=0.001) groups, they were significantly lower than the Sh group. MDA levels in the HIIT+CA group were significantly lower than the HIIT (P=0.001) and CA (P=0.003) groups (Figure 2).     PC levels in the C and Sh groups were not significantly different (P=0.23), however, in the HIIT (P=0.001), CA (P=0.001) and HIIT+CA (P=0.001) groups, they were significantly lower than the C and Sh groups. There was no significant difference between the CA (P=0.99) and HIIT+CA (P=0.91) groups compared to the HIIT group; Also, PC levels in the HIIT+CA group were not significantly different from the CA group (P=0.93) (Figure 3).   Discussion The results showed that HIIT increased GPx and decreased PC levels in the soleus muscle of elderly rats. HIIT with the mechanism of instant response to oxidative stress (OS) induced by exercise modulates cell redox increases protein kinases, the expression of respiratory transcription proteins (NRF1/2) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1), and modulates nuclear factor-κB (NF-κB). It also increases SOD and GPx expression and decreases PC and MDA (8, 14); Moreover, consumption of CA and HIIT + CA with the mechanism of increasing the expression of forkhead box O (FOXO) protein, activation of PI3K pathway, AKT1/2, IGF-1, increasing of PGC-1α, modulation of NRF1/2, NF-κB, increasing of protein synthesis and antioxidants enzymes in mitochondria and cytosol increases GPx and decreases MDA and PC in the soleus muscle tissue of elderly rats (9, 16).   Conclusion It seems that HIIT and CA consumption separately and synergistically have a beneficial effect on reducing oxidative stress and increasing antioxidant activity, however, further studies in this regard seem necessary. Manuscript profile

Open Access Article

36 - Comparison of the effect of silicon and nano-silicon on some biochemical and photosynthetic traits of Zea mays L. under salinity stress
Mahbobeh Zarooshan Ahmad Abdilzade Hamid Reza Sadeghipour Pooyan Mehrabanjoubani

Salinity stress is one of the factors that threatens the growth and yield of corn. The effects of silicon or nanosilicon on salinity alleviation have been reported in some plants. The effects of foliar application of silicon (inorganic and nanopatricle) on mitigation of More

Salinity stress is one of the factors that threatens the growth and yield of corn. The effects of silicon or nanosilicon on salinity alleviation have been reported in some plants. The effects of foliar application of silicon (inorganic and nanopatricle) on mitigation of the effects of salinity stress on some photosynthetic and biochemical parameters were studied in corn. Experiments were conducted in a completely randomized design with factorial arrangement in a pot culture. The first factor was salinity at two levels including 0 and 100 mM NaCl and the second factor was silicon at three levels including control (without silicon) and 2 mM potassium silicate and 2 mM nanosilicon (SiO2). Under salinity stress, the fresh and dry weights of shoots and roots, the chlorophylls and carotenoids contents, and the amount of soluble protein decreased significantly compared to the control. In contrast, the amount of hydrogen peroxide and malondialdehyde increased in these plants. Also, the photosyntheic rate and water use efficiency of plants decreased under salinity. The application of silicon and nanosilicon improved the growth of plants under salinity and increased the photosynthetic rate and the amount of photosynthetic pigments. In addition, severe reduction in transpiration under silicon application compared to nanosilicon resulted in the increased water use efficiency in this treatment. Silicon application also increased the activity of soluble peroxidase enzyme in plants under salinity, a result which was not observed with nanosilicon application. This resulted in a further decrease in hydrogen peroxide and lipid peroxidation of plants under salinity treated with silicon compared to nanosilicon, which showed a further decrease in oxidative stress in this treatment. These findings indicated that stress reduction and growth improvement of the plants under salinity with silicon application were more than the treatment with nanosilicon. Manuscript profile

Open Access Article

37 - Changes in germination indices and the activity of antioxidant system of cottonseed (Gossypium hirsutum L.) during deterioration
Farshid Ghaderifar omid sancholi Hamidreza sadeghipour

10.30495/iper.2021.679513

Seeds deteriorate and become aged during storage so that the rate of this process depends on the temperature and seed moisture content. This experiment was conducted to study changes in the activity of antioxidant system of cottonseeds during deterioration. An Accelerat More

Seeds deteriorate and become aged during storage so that the rate of this process depends on the temperature and seed moisture content. This experiment was conducted to study changes in the activity of antioxidant system of cottonseeds during deterioration. An Accelerated aging test was used to create different levels of deterioration. Cottonseeds were incubated at 43 ℃ for 0, 48, 96, 144, and 192 hours and 100% relative humidity. Results showed that the membrane electrical conductivity, lipid peroxidation, and hydrogen peroxide increased with lengthening of deterioration periods. Increase in hydrogen peroxide was accompanied with decreased activity of catalase and the increased activity and content of peroxidase and ascorbic acid, respectively, which indicates declined activity of catalase due to aging as compared with peroxidase activity and ascorbic acid content. Also, with an increase in the period of deterioration, percent, rate, and uniformity of germination reduced. In general, the study indicated that the oxidative stress due to the accumulation of reactive oxygen species and the reduction of the antioxidant system is one of the main reasons for cottonseed viability loss during storage. Manuscript profile

Open Access Article

38 - The effects of gibberellic acid on certain physiological parameters in alfalfa (Medicago sativa L.) under cadmium stress.
Farzaneh Najafi Zeinab Taghizadeh

10.30495/iper.2022.690252

Alfalfa is one of the most important forage corps in the world. The Medicago plant is a major genus from the fabaceae.High concentration of heavy metals in soil is one of the most important problems in the environment . Cadmium is a heavy metal that can cause oxidative More

Alfalfa is one of the most important forage corps in the world. The Medicago plant is a major genus from the fabaceae.High concentration of heavy metals in soil is one of the most important problems in the environment . Cadmium is a heavy metal that can cause oxidative stress in plant cells. In this research effects of different concentrations of cadmium (0 , 25 and 50 µM) and gibberellic acid (0 , 5 and 10 µM) were investigated on certain physiological parameters in Medicago sativa L. Alfalfa seeds were sterilized and cultured in pots containing sand which were irrigated with Hoagland nutrient solution. plants were treated with cadmium chloride and gibberellin 22 - old day.after twenty two days Plants were harvested to assay some physiological parameters. All treatments were conducted with four replications. Experiment results showed that increasing cadmium chloride concentration in Hoagland nutrient solution, decreased growth parameters and pigment contents and GPX activity.But , but proline contents, SOD and CAT activties in roots and leaves of plants increased. by additionof gibberellin concentrations. . It is concluded that gibberellic acid could alleviate the adverse effects of stress cadmium chloride in Medicago sativa L. Manuscript profile

Open Access Article

39 - Effect of salicylic acid in alleviating Ni-induced oxidative stress in wheat (Triticum aestivum L.) at germination stage
A.R Jafari, R.A Khavarinejad حمید Fahimi

The effects of salicylic acid (SA) in alleviating Ni-induced oxidative stress in wheat (Triticum aestivum L.) at germination stage were studied and the changes in radicle and plumule growth (fresh and dry weight and their lengths) were investigated. Exposure to 800&micr More

The effects of salicylic acid (SA) in alleviating Ni-induced oxidative stress in wheat (Triticum aestivum L.) at germination stage were studied and the changes in radicle and plumule growth (fresh and dry weight and their lengths) were investigated. Exposure to 800µM Ni significantly decreased radicle and plumule growth, and activities of catalase (CAT) and peroxidase (POD) enzymes but increased the concentration of malondialdehyde (MDA). However, treatment with 100µM SA enhanced the activities of these enzymes in plumule tissues but lowered the concentration of MDA in the Ni-stressed wheat compared with the Ni treatment alone. Treatment with SA alleviated the Ni-induced inhibition of radicle and plumule growth.The results showed that treatment with SA enhanced the antioxidant defense activities in Ni-stressed wheat, thus alleviating Ni-induced oxidative damage and enhancing Ni tolerance. Manuscript profile

Open Access Article

40 - The effecte of eight weeks of resistance training with royal jelly on the pathaphysiological changes in the hippocampal tissue of Alzheimer's rats
Leila Mokhtari Tahereh Bagherpour Nematollah Nemati

Abstract Introduction: Oxidative stress plays role in the progression of Alzheimer's disease (AD).exercise and antioxidants on neuronal health have effect . the effect of eight weeks of resistance training (RT) along with royal jelly (RJ) on Malondialdehyde (MDA) More

Abstract Introduction: Oxidative stress plays role in the progression of Alzheimer's disease (AD).exercise and antioxidants on neuronal health have effect . the effect of eight weeks of resistance training (RT) along with royal jelly (RJ) on Malondialdehyde (MDA), Protein Carbonylate (PC) and the pathological changes in the hippocampal tissue of Alzheimer's rats treated with trimethyltin (TMT)has been determined. Methods: In this experimental study, 42 male Sprague-Dawley rats treated with 8 mg/kg TMT were divided into TMT, Sham, RJ100, RJ200, RT, RT+RJ100 and RT+RJ200 groups. Supplemental groups received RJ with doses of 100 and 200 mg/kg/day as peritoneal injection, and the increasing RT protocol was performed for 8 weeks, 3 sessions a week with an intensity of 30 to 100% of the weight. Results: MDA, PC in TMT group were significantly higher than HC group. But MDA and PC in RT+RJ200 group were lower than TMT group (P≤0.05). Also, PC in RT group was significantly lower than TMT. MDA in RJ200 group were lower than TMT; PC values in RJ100 group was significantly lower than TMT (P≤0.05). The reducing effect of PC in RJ100 group was more favorable than RJ200 (P≤0.05). Also, the effect of reducing MDA in the RT+RJ200 group was more favorable than the RT+RJ100 group (P≤0.05). Conclusion:the resistance training and royal jelly have favorable effects on reducing oxidative stress. the simultaneous effect of these two interventions, especially with a higher dose, has a more favorable effect on reducing oxidative stress in the hippocampus tissue in AD modeling conditions. Manuscript profile

Open Access Article

41 - The Effect of Oxidative Stress on Proteomics of Listeria monocytogenes PTCC 1297
Samaneh Kazemi Manoosh Zandehdel

10.30495/bioem.2022.696575

Listeria monocytogenes is a ubiquitous Gram-positive food-borne human bacterial pathogen that can cause listeriosis. This disease is fatal with a high rate of hospitalization (>90%). This study aims to determine the effect of hydrogen peroxide (H2O2) on L. monocytoge More

Listeria monocytogenes is a ubiquitous Gram-positive food-borne human bacterial pathogen that can cause listeriosis. This disease is fatal with a high rate of hospitalization (>90%). This study aims to determine the effect of hydrogen peroxide (H2O2) on L. monocytogenes PTCC 1297 proteomics. Bacterial cells exposed to gradually increasing sub-lethal concentrations of oxidative stress: 0.06, 0.3, 0.6, and 1.5 % of H2O2. Changes in the protein profile of cells exposed to H2O2 and control (non-adapted cells) were determined by isoelectric focusing (IEF) and sodium dodecyl sulfate– polyacrylamide gel electrophoresis (SDS-PAGE). Protein spots are visualized by staining with colloidal Coomassie Brilliant Blue. Based on the obtained results, one thousand four hundred spots were identified on the gels. Out of these points, 85 spots were reproducibly detected with the help of software and eye confirmation. After analyzing these points, 9 spots showed the most significant changes. These points had appropriate density and indicated the response of bacteria to stress conditions. It can be concluded that the structure of the proteome of L. monocytogenes PTCC 1297 changes when faced with oxidative stress. Nine protein spots on the gel were found to have substantial variations after protein spot analysis. These sites show how bacteria react under stress. Some of these spots' expressions had increased, while others had decreased. Manuscript profile

Open Access Article

42 - اثر الاژیک اسید بر استرس اکسیداتیو ناشی از ایسکمی/هیپوپرفیوژن مغزی در موش صحرایی نر
مریم رفیعی راد خدیجه قاسم زاده دهکردی

مقدمه و هدف: رادیکال های آزاد در طی فرآیندهای ایسکمی تولید می شوند. رادیکال های آزاد در بیماری های عصبی نقش دارند و آنتی اکسیدان ها نقش محافظتی دارند. الاژیک اسید یک ترکیب پلی فنلی با خواص آنتی اکسیدانی است که در میوه هایی مانند انار، زغال اخته و انواع توت یافت می شود. More

مقدمه و هدف: رادیکال های آزاد در طی فرآیندهای ایسکمی تولید می شوند. رادیکال های آزاد در بیماری های عصبی نقش دارند و آنتی اکسیدان ها نقش محافظتی دارند. الاژیک اسید یک ترکیب پلی فنلی با خواص آنتی اکسیدانی است که در میوه هایی مانند انار، زغال اخته و انواع توت یافت می شود. این مطالعه با هدف بررسی اثر 14 روز تجویز خوراکی الاژیک اسید 50 میلی گرم بر کیلوگرم بر شاخص های استرس اکسیداتیو مغز پس از انسداد دائمی دو طرفه شریان کاروتید مشترک و یا ایسکمی/ هیپوپرفوزیون در موش‌های صحرایی نر بالغ انجام‌گرفت. مواد و روش‌ها: تعداد 30 سر موش به گروه های آزمون و کنترل تقسیم شدند. برای ایجاد ایسکمی در موش صحرایی، شریان های کاروتید عمومی بوسیله ابزارهای بخیه ی پوستی با دو گره محکم در حول رگ (بالا و پایین) مسدود و سپس شریان ها به طور کامل از وسط قطع گردیدند. سپس مغز موش‌ها جهت ارزیابی میزان پراکسیداسیون لیپیدی و اندازه گیری میزان گروه های تیول(-SH)  استخراج شدند. نتایج و بحث: نتایج تحقیق حاضر نشان داد که میزان مالون دی آلدئید و تیول در گروه ایسکمی نسبت به گروه کنترل افزایش معنی داری داشته است و میزان مالون دی آلدئید و تیول در گروه ایسکمی دریافت کننده الاژیک اسید نسبت به گروه ایسکمی کاهش معنی داری داشت. توصیه کاربردی/صنعتی: الاژیک اسید با خاصیت آنتی اکسیدانی قوی توانسته‌است اثرات ایسکمی ازجمله تولید رادیکال‌های آزاد را بهبود ببخشد. Manuscript profile

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43 - پتانسیل عصاره Aju Mbaise (AME) بر بهبود استرس اکسیداتیو و آسیب کبدی ناشی از دوتاستراید در موش صحرایی
رابرت اوروکو الیشا اوگو پل نیوجی-آنیالوو ایکنا اوبیورو چینومسو هارون اوبینا امبا

10.30495/medherb.2023.700160

Background & Aim: Aju Mbaise is a polyherbal extract with nutraceutical properties that helps to replenish the volume of blood lost during childbirth and improves breast milk secretion and the general wellbeing of the mother. This study evaluated the ameli More

Background & Aim: Aju Mbaise is a polyherbal extract with nutraceutical properties that helps to replenish the volume of blood lost during childbirth and improves breast milk secretion and the general wellbeing of the mother. This study evaluated the ameliorative potentials of Aju Mbaise extract (AME) on Dutasteride-induced oxidative stress and hepatic injury in rats. Twenty-one rats were used to assess the acute toxicity of AME.Experimental: The study for the hepatoprotective effects of AME had five groups of rats, including normal control, Dutasteride only, AME only, Dutasteride + AME (500 mg/kg) and Dutasteride+ AME (1000 mg/kg).Results: The acute toxicity result showed that AME is relatively safe for consumption. Dutasteride caused significant elevation of liver marker enzymes, including aspartate transaminase (AST), alanine transaminase (ALT), transaminase (AST), alkaline phosphatase (ALP), total bilirubin, malondialdehyde (MDA) and significantly reduced catalase, superoxide dismutase (SOD), glutathione peroxidase (GPx), reduced glutathione (GSH), total proteins, albumin, and globulin levels in the rats received only Dutasteride. In contrast, Dutasteride induced rats treated with AME showed a significant decline in the AST, ALT, ALP, MDA, and bilirubin and significantly increased SOD, GSH, GPx, total proteins, albumin, and globulin levels compared to Dutasteride induced untreated rats. The AME-treated rats showed normal liver histo-architecture, unlike the Dutasteride-induced untreated rats that showed mild to moderate vacuolar degeneration of the hepatocytes.Recommended applications/industries: The findings show that AME ameliorates Dutasteride caused rats oxidative stress and hepatic injury. Manuscript profile

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44 - بررسی سم شناسی عصاره اتانولی دانه Citrus paradisi Macfad (گریپ فروت) بر وضعیت اکسیداتیو ، عملکرد اندام و وضعیت بافت در موش صحرایی ویستار
گودویل اودوم اومینی یمیتان دانیال اوبوت جون ادوبانگ نکچی اونیکو امانوئل ابانگو ساندی سویمی

10.30495/medherb.2021.681956

Background & Aim:Citrus paradisi Macfad (Rutaceae) seed extract (CPE) is used in folkloric medicine for the management of diabetes, blood deficiencies and as immune booster, which may require long term usage. This study aimed to evaluate the toxicity profile of etha More

Background & Aim:Citrus paradisi Macfad (Rutaceae) seed extract (CPE) is used in folkloric medicine for the management of diabetes, blood deficiencies and as immune booster, which may require long term usage. This study aimed to evaluate the toxicity profile of ethanol seed extract of grapefruit in adult Wistar rats in order to determine its safety profile in whole organism’s systems. Experimental: Acute toxicity study was conducted using OECD–425 procedure. Subchronic toxicity study (90 days) was conducted using 40 adult male Wistar rats, randomly divided into four groups (10/group) and orally-treated daily, thus: Group I-Control (normal saline, 10 ml/kg), Groups II-IV received CPE (4, 40 and 400) mg/kg body weight, respectively. On the 92nd day, animals (6/group) were euthanized under diethyl ether anaesthesia and sacrificed. Vital organs were eviscerated, blotted, weighed and stored for oxidative stress measurement; some samples of the organs were fixed in formalin for histopathological examination. Other animals (4/group) were retained for reversibility studies. Results: Results showed significant increase and decrease in weights of the kidneys and spleen, respectively. Significant increase in malondialdehyde level and decreases in superoxide dismutase, glutathione and catalase activities were recorded. Histopathology of the kidney, liver, and lungs showed some degree of pathologies. Reversibility studies showed reversal of test effects on extract discontinuation. Despite the diverse biological usefulness of ethanol seed extract of C. paradisi (CPE), it may also induce an array of toxicities especially on long term use. Recommended applications/industries: Contrary to the tradomedical claims that CPE is absolutely safe, the study revealed that CPE may induce oxidative stress and organ toxicity especially on long term use. It is imperative the plant seed extract and its derivatives be used with utmost caution, and where possible be avoided.   Manuscript profile

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45 - خواص درمانی عصاره متانولی برگ Momordica charantia در سمیت های قلبی ناشی از آلوکسان در موش صحرایی
ساندی افوب آدمولا اویابمی تمیدایو اومواله ادورابنرو آدداپو آبیدون آیودل مومح یاکوبو الوفمی اگانتیبجو آدلو آدداپو

Background & Aim:Momordica charantiais an extensively distributed plant that is broadly prescribed in African medical system for treatment of various ailments. The plant has a comprehensive range of therapeutic uses. The present study reveals the antioxidative and c More

Background & Aim:Momordica charantiais an extensively distributed plant that is broadly prescribed in African medical system for treatment of various ailments. The plant has a comprehensive range of therapeutic uses. The present study reveals the antioxidative and cardioprotective abilities of methanol leaf extract of Momordica charantia (MLEMC) against cardiotoxicity using alloxan-induced animal model. Experimental: The ameliorative effect of the methanol leaf extract of Momordica charantia (MLEMC) was studied in alloxan-induced cardiac injury in 50 rats divided into five groups (A-E) (n =10) i.e. group A control, group B was toxicant group, group C animals received glibenclamide treatment while groups D and E received extracts at 200 and 400 mg/kg doses, respectively, for 28 days. Histopathological changes, serum cardiac injury markers such as myeloperoxidase (MPO) activity, nitric oxide (NO) contents; oxidative status, blood pressure, electrocardiogram, cardiac P38 and CRP were evaluated. Results: The extract-treated group showed a decreased level of oxidant markers such as malondialdehyde(MDA) and hydrogen peroxide(H2O2) but increased levels of protein thiols, non-protein thiols, glutathione (GSH), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and superoxide dismutase (SOD) indicating its anti-oxidant potential. Heart sections revealed mild distortion of the cardiac architecture compared to toxicant group while decreased expression of cardiac P38 and CRP in extract-treated groups was observed. Recommended applications/industries: The plant extract exhibited anti-oxidant and anti-inflammatory effects, thereby displaying cardio-protective property which propose the plant as a good natural source for herbal nutraceuticals. Manuscript profile

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46 - بررسی اثر حفاظتی اسپیرولینا، عصاره چای سبز و ویتامین E در مقابل استرس اکسیداتیو ناشی از کادمیوم بر روی مغز رت
امیر علی شهبازفر سید جمال امامی رضا اسدپور

مقدمه و هدف: امروزه جلبک اسپیرولینا و چای سبز به عنوان یکی از مواد با فواید تغذیه ای فراوان شناخته شده اند. در تحقیق حاضر اثرات حفاظتی این دو ماده در جلوگیری از آسیب اکسیداتیو ناشی از کادمیوم بر روی مغز موش سوری مورد بررسی قرار گرفت و نتایج با اثرات  حفاظتی ویتامین More

مقدمه و هدف: امروزه جلبک اسپیرولینا و چای سبز به عنوان یکی از مواد با فواید تغذیه ای فراوان شناخته شده اند. در تحقیق حاضر اثرات حفاظتی این دو ماده در جلوگیری از آسیب اکسیداتیو ناشی از کادمیوم بر روی مغز موش سوری مورد بررسی قرار گرفت و نتایج با اثرات  حفاظتی ویتامین E به عنوان یک انتی اکسیدان های  قوی و شناخته شده مقایسه شد. روش تحقیق:روش تحقیق: بدین منظور تعداد&lrm;&lrm;&rlm;30&rlm;&lrm;&lrm;سر&lrm;&lrm;رت&lrm;&lrm;ماده&lrm;&lrm;بالغ&lrm;&lrm; &lrm;به طور&lrm;&lrm;مساوی&lrm;&lrm;و&lrm;&lrm;تصادفی&lrm;&lrm;به&lrm;&lrm;پنج&lrm;&lrm;گروه&lrm;&lrm;تقسیم&lrm;&lrm;شدندو به مدت چهار هفته تحت رژیم درمانی قرار گرفتند. کنترل ، کادمیوم (مخلوط با جیره)، کادمیوم و محلول اسپیرولینا (محلول در آب)، کادمیوم وعصاره چای سبز ( محلول در آب)، کادمیوم و ویتامین E ( تزریق داخل صفاقی). در انتهای تحقیق موش ها به روش انسانی کشته شدند و مغز ها جهت تثبیت سازی در فرمالین انداخته شد. هیپوکامپ، caudate putamen، corpus callosum، قشر مخ و مخچه با میکروسکوپ نوری جهت مشاهده و بررسی ضایعات مورد مطالعه قرار گرفتند. نتایج و بحث: تغییرات پاتولوژیکی از قبیل نوروفاژی ، پرخونی، خون ریزی، نکروزو آتروفی نورون ها، کروماتولیز مزکزی، نکروز لایه ای و اسفنجی شدن در نواحی مختلف مغز مشاهده شد. هنگام استفاده از کادمیوم به تنهایی قطر لایه مولکولار و گرانولر مخچه و همچنین اندازه سلول های پورکنژ به طور قابل توجهی کاهش یافت. هنگام استفاده از اسپیرولینا همراه با کادمیوم تغییری در سلول های پورکنژ مشاهده نشد و تغییرات لایه های مخچه بسیار ناچیز بود (P<0.05). هم چنین ، اسپیرولینا شمار سلول های نکروز شده در هیپوکمپ و قشر مخ را به شدت کاهش داد. اسپیرولینا در مقایسه با عصاره چای سبز و ویتامین E اثر حفاظتی قوی تری در مقابل کادمیوم از خود نشان داد. عصاره چای سبز کمترین اثر را داشت. هیپوکمپ بیش از سایر نواحی مورد بررسی دچار ضایعات شده بود. توصیه کاربردی/ صنعتی: نتایج نشان می دهند که اسپیرولینا یک منبع خوب از آنتی اکسیدان با خواصی حتی بهتر از ویتامین E می باشد و می تواند به تنهایی یا همراه با دیگر آنتی اکسیدان ها جهت جلوگیری از آثار مخرب ترکیبات اکسیداتیو مورد استفاده قرار گیرد. Manuscript profile

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47 - فعالیت آنتی اکسیدانی عصاره متانولی Uapacato genensis در موشهای تحت تاثیر Plasmodium berghei NK65
امینا بوسولا اولوروکوبا بن احمد چیدو یحیی محمدثانی

Background & Aim: Oxidative stress has been linked to the development of systemic complications in malaria infection. Recent approaches in treatment of malaria suggest that the control of oxidative stress in infected patients may be of therapeutic advantage. Th More

Background & Aim: Oxidative stress has been linked to the development of systemic complications in malaria infection. Recent approaches in treatment of malaria suggest that the control of oxidative stress in infected patients may be of therapeutic advantage. The stem bark and leaves of Uapaca togoensis are used locally in the treatment of various diseases including malaria. This study aimed to evaluate the antioxidant activities of the methanol stem bark extract of the plant in Plasmodium berghei infected mice. Experimental: Mice were inoculated intraperitoneally with 0.2 ml of parasitized erythrocytes and parasitemia level assessed after 72 h by the preparation of thin blood films stained with Geimsa stain. The mice were divided into five groups of six mice each. Groups I and V were administered with distilled water (10 ml/kg) and chloroquine (5 mg/kg) orally for four days. The extract at doses of 250, 500 and 1,000 mg/kg were orally administered to groups II, III and IV, respectively, for the same period. Mice were sacrificed under light chloroform. Blood was collected by cardiac puncture and centrifuged at 2,500 rpm for 15 minutes to obtain the serum. The serum was then analyzed to determine the levels of glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and lipid peroxidation product (malondialdehyde - MDA). Results: Oral median lethal dose of the methanol stem bark extract of Uapacatogoensis was estimated to be greater than 5,000 mg/kg. Administration of the extract to P. berghei infected mice produced a significant (p<0.05) increase in superoxide dismutase, reduced glutathione and catalase levels in. However, a significant (p<0.05) decrease in lipid peroxidation activity in the parasitized mice was observed. Recommended applications/industries: The plant possesses antioxidant property which can be exploited in the .management of oxidative stress caused by malaria    Manuscript profile

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48 - Toxic Impact of Sub-Chronic Oral Exposure of Cyfluthrin on Antioxidant Status in Buffalo Calves
R. Kaur S. Ahsan-UI-Haq B. Ranjan S. Rampal

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49 - Performance and Oxidative Stress Biomarkers of West African Dwarf Goats Fed Diet Containing Incremental Sodium Humate
T.T. Ikyume A.O. Yusuf A.O. Oni O.S. Sowande S. Ikuejamoye-Omotore S.S. Dansu

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50 - اثرات تغذیه زردچوبه، دارچین، زنجبیل و پودر سیر بر وضعیت آنزیم‌های آنتی‌اکسیدانی و هورمون‌های مؤثر بر متابولیسم انرژی در جوجه‌های گوشتی تحت تنش گرمایی
ع.ا. صادقی م. مقدم

آزمایش حاضر به منظور تعیین اثرات زردچوبه، دارچین، زنجبیل و پودر سیر بر عملکرد، وضعیت آنتی‌اکسیدانی و هورمون‌های تیروئیدی سرم خون در جوجه‌های گوشتی سویه کاب-500 جنس مخلوط انجام گردید. تنش حرارتی در 42-31 روزگی از ساعت 12 صبح الی 16 بعدازظهر به وسیله افزایش طبیعی دمای هوا More

آزمایش حاضر به منظور تعیین اثرات زردچوبه، دارچین، زنجبیل و پودر سیر بر عملکرد، وضعیت آنتی‌اکسیدانی و هورمون‌های تیروئیدی سرم خون در جوجه‌های گوشتی سویه کاب-500 جنس مخلوط انجام گردید. تنش حرارتی در 42-31 روزگی از ساعت 12 صبح الی 16 بعدازظهر به وسیله افزایش طبیعی دمای هوای محیطی ایجاد گردید. نمونه‌های سرم خون از 6 جوجه 42 روزه برای هر تیمار تهیه گردیدند و برای سنجش فعالیت آنتی‌اکسیدانی آنزیم‌های کبدی و هورمون‌های تیروئیدی مورد استفاده قرار گرفتند. همه جوجه‌های گوشتی تغذیه شده با جیره‌های حاوی گیاهان دارویی افزایش وزن روزانه و وزن طحال بیشتری نسبت به تیمار شاهد داشتند. مکمل‌های گیاهان دارویی سبب افزایش فعالیت آنزیم‌های سوپراکسیداز دیسموتاز (SOD) در دارچین، گلوتایتون پراکسیداز (GPX) در زنجبیل، کاتالاز (CAT) در دارچین و پودر سیر، ظرفیت تام آنتی‌اکسیدانی (TAC) در دارچین، آلکالین فسفاتاز (ALP) در پودر سیر و کورتیکواستروئید (CE) در دارچین شدند. همچنین مکمل دارچین سبب کاهش غلظت مالون دی آلدئید (MDA) گردید (05/0>P). غلظت هورمون‌های تیروئیدی (THs) در جوجه‌های گوشتی 42 روزه تغذیه شده با مکمل‌های گیاهان دارویی نسبت به تیمار شاهد افزایش یافت (05/0>P). مکمل‌های زردچوبه و زنجبیل سبب کاهش معنی‌دار ضریب تبدیل غذایی، بهبود عملکرد، و مصرف دارچین سبب افزایش فعالیت آنزیم‌های آنتی‌اکسیدانی گردیدند. همچنین، مصرف زردچوبه و زنجبیل، هورمون‌های تیروئیدی در جوجه‌های گوشتی را افزایش دادند. Manuscript profile

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51 - Morus nigra Fruit Extract Safeguards Frozen Thawed Bovine Sperm Parameters
S. Suleman M.A. Kanwal R. Ali N. Kanwal S. Yasmeen M.Y. Mehmood S. Siddique S.N. Ahmad A. Younis I. Inayat K. Raees Ahmad

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52 - Metal-Induced Oxidative Stress and Cellular Signaling Alteration in Animals
س. لوساکو آ. پریلو

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53 - Effects of Dietary Supplementation of Saccharomyces cerevisiae on Milk Production, Oxidative Stress, and Blood Metabolites of Holstein Dairy Cows during Summer Season
F. Sehati A. Towhidi M. Zhandi M. Ganjkhanlou A.H. Nasiri F. Parnian-Khajehdizaj

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54 - Antioxidant enzyme responses and crop yield of wheat under drought stress and re-watering at vegetative growth period.
Mohsen Saeidi Shiva Ardalani Saeid Jalali-Honarmand Mohammad-Eghbal Ghobadi Majid Abdoli

10.30495/ijpp.2017.539069

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55 - Sodium nitroprusside and salicylic acid decrease antioxidant enzymes activity in soybean
Leila Aalam Mohammad Sedghi Omid Sofalian

10.30495/ijpp.2019.670792

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56 - Influence of salicylic and jasmonic acids on the antioxidant systems of tomato (Solanum lycopersicum cv. Superchief) plants under biotic stresses.
Sahar Gholi-Tolouie Mahdi Davari Nemat Sokhandan-Bashir Mohammad Sedghi

10.30495/ijpp.2018.539110

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57 - Alleviation of oxidative stress induced by drought stress through priming by β-aminobutyric acid (BABA) in Rapeseed (Brassica napus L.) plants.
Neda Mohamadi Amin Baghizadeh Sara Saadatmand Zahra Asrar

10.30495/ijpp.2017.537988

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58 - Effects of priming with salicylic acid on germination traits of Dracocephalum moldavica L. under salinity stress
Fatemeh Shaikh-Abol-hasani Parto Roshandel

10.30495/ijpp.2019.670789

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59 - Physiological and phytochemical changes induced by seed pretreatment with hydrogen peroxide in Artemisia sieberi under salt stress.
Forouzan Azimian Parto Roshandel

10.30495/ijpp.2016.532427

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60 - اثر نانواکسید روی، اسید سالیسیلیک و سدیم نیتروپروساید روی صفات فیزیولوژیکی و آنزیمی بنفشه معطر تحت رژیم‌های آبی مختلف
حمیده باقری داود هاشم‌آبادی بهمن پاسبان اسلام شهرام صداقت حور

 اثرات نانواکسید روی (ZnO)، اسید سالیسیلیک (SA) و سدیم نیتروپروساید (SNP) روی بنفشه معطر در شرایط مختلف آبیاری بهصورت آزمایش فاکتوریل مورد مطالعه قرار گرفت. عوامل عبارت بودند از تنش خشکی در سه سطح شامل آبیاری در 55 (تنش شدید)، 65 (تنش ملایم) و 85 (شاهد) درصد ظ More

 اثرات نانواکسید روی (ZnO)، اسید سالیسیلیک (SA) و سدیم نیتروپروساید (SNP) روی بنفشه معطر در شرایط مختلف آبیاری بهصورت آزمایش فاکتوریل مورد مطالعه قرار گرفت. عوامل عبارت بودند از تنش خشکی در سه سطح شامل آبیاری در 55 (تنش شدید)، 65 (تنش ملایم) و 85 (شاهد) درصد ظرفیت مزرعه (FC) بر اساس تخلیه آب خاک و استفاده برگی ZnO، SA، SNP  و آب مقطر (به عنوان شاهد) در 7 سطح. مقایسه میانگین‌ها نشان داد که بهترین نتایج از اثر متقابل 200 میلی‌گرم در لیتر SA و آبیاری در سطح 85 درصد FC بهدست آمد و این تیمار روی اکثر صفات اثر مثبت داشت. همچنین تیمار 300 میلی‌گرم در لیتر و هر دو سطح SNP نسبت به شاهد و نانواکسید روی بهتر بودند. کمترین هدایت روزنه‌ای در SNP 200 میکرومولار در 55 درصد ظرفیت مزرعه بهدست آمد. کمترین مالوندی آلدئید (MDA) در تیمار 200 میلی‌گرم در لیتر SA و 85 درصد FC حاصل شد. بیشترین مقدار رطوبت نسبی (RWC) در تیمار 1500 میلی‌گرم در لیتر نانواکسید روی و 85 درصد ظرفیت مزرعه دیده شد. گیاهان شاهد در 55 درصد FC بیشترین پرولین (62/46 میکروگرم در گرم وزن تر) را داشتند. بالاترین فعالیت آنزیم پراکسیداز (POD) مربوط به تیمار 1000 میلی‌گرم در لیتر نانو اکسید روی و بیشترین فعالیت آنزیم آسکوربات پراکسیداز (APX) در تیمار 1500 میلی‌گرم در لیتر نانواکسید روی در 55 درصد ظرفیت مزرعه بهدست آمد. Manuscript profile

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61 - Study of the effect of oxidative stress on Nrf-2 cell signaling pathway in mesenchymal stem cells (MSCs)
parisa bostani elnaz khojaste changiz ahmadizadeh

The study of mesenchymal stem cells showed that induction of autophagy in MSCs increased sensitivity to oxidative stress Maharatophagy induced resistance of MSCs to oxidative stress and increased survival of MSCs compared to control cells. The aim of this study was to i More

The study of mesenchymal stem cells showed that induction of autophagy in MSCs increased sensitivity to oxidative stress Maharatophagy induced resistance of MSCs to oxidative stress and increased survival of MSCs compared to control cells. The aim of this study was to investigate the effect of oxidative stress on the Nrf2 cell signaling pathway in mesenchymal stem cells. The present study was conducted at the Pharmaceutical Micro-Technology Research Center of Tabriz Medical Sciences in 2017. In this experimental study, mesenchymal stem cells were cultured in DMEM-F12 medium and after trypsinization, re-culture was performed and the fourth cell passage was selected. Then it was examined using MTT assay method. DNA extraction was performed from treated cells and DNA Ladder assay was performed. After cDNA preparation, the expression of Nrf2 genes in mesenchymal stem cells was measured by real-time PCR. Data analysis was performed using SPSS software. done. The results showed that oxidative stress could activate the Nrf2-Nngoi cell signaling pathway and that stem cells could inhibit stress through this pathway. Therefore, it is necessary to strengthen this pathway through dietary antioxidants and supplements to increase the effectiveness of cell therapy. Manuscript profile

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62 - Bioreduction of environmental Al by Lisianthus plant
Faeze Ghanati Zeynab Hashemi

Aluminum is a trivalent metal which due to its abundance in the earth's crust is one of the major environmental problems.Cation Al3+ in acidic soils is a key factor in the decline of agricultural production. Abundance of Al in water, air and soil around us results in it More

Aluminum is a trivalent metal which due to its abundance in the earth's crust is one of the major environmental problems.Cation Al3+ in acidic soils is a key factor in the decline of agricultural production. Abundance of Al in water, air and soil around us results in its uptake and accumulation in the body. Aluminum consumption in the form of antacids and drugs with aluminum hydroxide, Al presence in drinking water and food consumption for baking powder or cheeses is very common. On the other hand may also cause diseases such as Alzheimer's, osteoporosis, calcium deficiency, acute neurological disease, anemia, headache, decreased kidney function and bladder, slurred speech and loss of memory.  Identifying plants bearing the ability to absorb high amounts of Al and continuous growth without toxicity can be considered as an effective step in refining the soil and water from this element. The aim of this study is the investigation on the Al uptake capacity of Lisianthus aluminum in a hydroponic system. For this purpose, Lisianthus seedlings in hydroponic culture were treated with 400, 600, 800 and 1200 µM of Al for 10 days. The results showed no significant increase in superoxide anion and hydrogen peroxide as an indicator of stress in the roots. As well as the punctual increase iron regenerative power (FRAP) in the group treated with aluminum compared to the control group suggesting a high antioxidant power Lisianthus against aluminum. . The results suggest that Lisianthus can be used to reduce the concentration of Al in the environment.   Manuscript profile

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63 - Comparison of the effect of phenanthrene and pyrene on germination, seedling growth and antioxidant enzymes activity on wheat (Triticum aestivum L.)
Ayyoub Sobhani Seyed Yahya Salehi Lisar Ali Movafeghi

The aim of this study was to evaluate the effects of phenanthrene and pyrene as two polycyclic aromatic hydrocarbon on germination, growth and some biochemical indices in bread wheat seedlings. Wheat seedlings were cultivated hydroponically and treated by 50, 100, 150 a More

The aim of this study was to evaluate the effects of phenanthrene and pyrene as two polycyclic aromatic hydrocarbon on germination, growth and some biochemical indices in bread wheat seedlings. Wheat seedlings were cultivated hydroponically and treated by 50, 100, 150 and 200 µM of phenanthrene and pyrene for 7 days. Activity of the superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX), as well as H2O2, MDA and photosynthetic pigments content, were measured. The results showed that both pollutants reduced germination percentage, so that the lowest germination percentage was phenanthrene (54.44%) at 200 μm. Phenanthrene more than pyrene reduced the content of photosynthetic pigmentation, the lowest percentage of chlorophyll a (51.54%), chlorophyll b (48.25%) and carotenoids (63.42%) were related to phenanthrene treatment (200 μM). Both pollutants significantly increased the content of hydrogen peroxide (7 times) and malondialdehyde (1.5 times). Increasing the concentration of both pollutants resulted in a significant decrease (up to 70%) in the activity of the three APX, POD and SOD enzymes, but only phenanthrene significantly reduced (49.12%) activity of the catalase. These results indicated that induction of oxidative stress is one of the main reasons for lower plant growth rate and other harmful outcomes in PAHs contaminated environments. Manuscript profile

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64 - Antioxidant enzyme activities and fermentation metabolism in the root of three wheat promising lines under waterlogging stress
Freshteh alizade vaskasi hemmatollah Pirdashti Ali Cherati_Araei Sara Saadatmand

Waterlogging stress has negative effects on the growth and yield of wheat plants, which recognizes the defense mechanism of the plant against waterlogging, can be valuable. In order to study the response of three wheat genotypes to different levels of waterlogging stres More

Waterlogging stress has negative effects on the growth and yield of wheat plants, which recognizes the defense mechanism of the plant against waterlogging, can be valuable. In order to study the response of three wheat genotypes to different levels of waterlogging stress, a factorial experiment was conducted based on a completely randomized design with five replications in Gharakhil (Ghaemshahr) Agricultural Station. The objective of this study was to investigate the effects of waterlogging (0, 7, 14 and 21 day) at tillering (ZG21) and stem elongation (ZG31) stages on the growth, total chlorophyll, proline, malondialdehyde (MDA), H2O2, activity of antioxidant enzymes, and enzymes of fermentation pathway in root of three wheat promising lines (N-93-19, N-93-9 and N-92-9). The results showed that waterlogging stress in both growth stages reduced the total chlorophyll and growth of all three genotypes, however, the highest contents of total chlorophyll and shoot and root dry weight were observed in N-92-9 genotype. Increased waterlogging stress increased the contents of proline, MDA, H2O2, activity of superoxide dismutase, catalase, alcohol dehydrogenase and lactate dehydrogenase and decreased peroxidase activity of root of genotypes in both growth stages compared to control treatments. The results of this experiment that N-92-9 genotype had better response than other two genotypes in all studied traits under waterlogging conditions and was introduced as a flood tolerant genotype. Manuscript profile

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65 - ان استیل سیتئین آمید و سیستم عصبی مرکزی: مطالعات انسانی و مدل های حیوانی
واحد ابراهیمی زعفر قلی نژاد

10.30495/jbcvm.2023.1978678.1036

ساختار مغز اولین سد در برابر دارورسانی موثر در درمان اختلالات عصبی است. استرس اکسیداتیو یک شرایط مهم در آسیب زایی اختلالات عصبی است-N .استیل سیستئین آمید (NACA)،  یک مشتق چربی دوست تر –N استیل سیستئین (NAC) که قابلیت زیادی برای درمان اختلال مبتنی بر اکسیداتیو More

ساختار مغز اولین سد در برابر دارورسانی موثر در درمان اختلالات عصبی است. استرس اکسیداتیو یک شرایط مهم در آسیب زایی اختلالات عصبی است-N .استیل سیستئین آمید (NACA)،  یک مشتق چربی دوست تر –N استیل سیستئین (NAC) که قابلیت زیادی برای درمان اختلال مبتنی بر اکسیداتیو به ویژه در مغز نشان داده است.  در این مطالعه، 83 مقاله مورد ارزیابی قرار گرفت  که  در عنوان آنها کلید واژه   N –"استیل سیستئین آمید" وجود داشت. مرور مقالات نشان داد  NACA دارای اثر محافظتی در برابر سمیت سموم و داروها است. بیشتر مقالات گزارش کردند که NACA  یک گزینه درمانی عالی برای آب مروارید و اختلالات چشمی است. مشکل هماتولوژیک و اختلالات مرتبط با کلیه هدف NACA است. سپس، ما بر روی کاربرد  NACA در سیستم عصبی تمرکز کردیم. به خوبی مستند شده است که اثرات زیستی NACA توسط خواص آنتی اکسیدانی به واسطه گروه -SH وساطتت می شود. زیست دسترسی و قدرت عمل NACA   به طور قابل توجهی بالاتر از NAC است. ساختار لیپوفیلیک سبب ایجاد نتایج امیدوارکننده  NACA در درمان اختلالات مغزی شامل تروما، سموم و سمیت انتقال دهنده های عصبی شده است. Manuscript profile

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66 - Induction of Oxidative Stress and Anatomical Changes by Polycyclic Aromatic Hydrocarbons in Medicago sativa L.
Leyla Jafari Maryam Khoshsokhan-Mozaffar Elahe vatankhah

10.22034/jchr.2018.544199

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67 - Subchronic Toxicity of a Terbufos-based Pesticide (Counter 15FC) in Adult Male Rats
Danielle Zali Chedjeu Faustin Pascal Manfo Tsague Edouard Akono Nantia Denis Zofou Jules Clement Nguedia Assob

10.22034/jchr.2021.1903415.1152

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68 - The Impact of Artemisia absinthium Hydro-ethanolic Extract on Oxidative Stress in the Brain in a Seizure Model Induced by Pentylenetetrazole in Mice
Roghayeh Rashidi Mohammad Reza Mahdinezhad Mohammad-Ali Sobhanifar Narges Marefati Hassan Rakhshandeh Mahmoud Hosseini

10.22034/jchr.2022.1935870.1364

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69 - The Study of Antioxidant and Cellular Toxicity Effects of Methanol, Ethyl Acetate, Aqueous and n-Hexane Extracts of Symphytum Kurdicum Plant
Shiva Khalil_moghaddam Atousa Aliahmadi Nastaran Jalilian Mohamad Aref Tabad

10.22034/jchr.2020.1902349.1147

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70 - Investigation of Seed Germination, Early Growth and Physio-Biochemical Parameters of Canola Seedling Exposed to Co3O4 Engineered Nanoparticles
Malihe Jahani Ramazan Ali Khavari-Nejad Homa Mahmoodzadeh Sara Saadatmand

10.22034/jchr.2020.1891185.1092

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71 - Evaluation of Blood Biochemical Parameters and Oxidative Stress Biomarkers in Common Carp (Cyprinus carpio) Exposed to Deltamethrin
Mehdi Banaei Mohsen Forouzanfar Mojtaba Jafarinia

10.22034/jchr.2022.1945633.1463

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72 - The Effects of Two Weeks Quercetin Supplementation in Adolescent Swimmer Girls on The Oxidative Stress and Inflammatory Mediator’s Response to Acute Swimming Session
Masoumeh Gholaman Maryam Khodabakhsh Nokola Zahra Ghorbani Ganjeh Shokoufeh Sokhanvardastjerdi Mandana Gholami Maryam Kasiri

10.22034/jchr.2021.1934089.1344

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73 - The Neurotoxic Impact of Formulated Pyrethroid Insecticide on the Substantia Nigra of Adult Wistar Rat
Princewill udodi Edwin NNADI Damian Ezejindu Emeka OKAFOR Ifechukwu Obiesie Charles Oyinbo Darlington Akukwu Godwin Uloneme

10.22034/jchr.2022.1938288.1392

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74 - The Influence of Zinc Oxide Nanoparticles on Blood Markers in Domestic Pigeons (Columba livia)
Mehran Arabi Hamid-Reza Naseri

10.22034/jchr.2021.1937480.1379

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75 - Oxidative Stress Responses and Toxicity of Green Synthesized Silver Nanoparticles (AgNPs) on Basil (Ocimum basilicum) Seedlings
Ali Zareii Hossein Abbaspour Maryam Peyvandi Ahmad Majd

10.22034/jchr.2023.1978538.1673

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76 - The Effect of Rosa Damascena Extract on Diazinon Toxicity in Mice
Oveys Pourmahdi Tohid Moradi Gardeshi Ali Amirkhani Majid Gholami-Ahangaran

10.22034/jchr.2021.1941985.1422

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77 - Patho-biochemical Markers of Renal Toxicity in Homing Pigeons (Columba Livia f. Urbana) Induced by Nano-ZnO Particles
Mehran Arabi Hamid-Reza Naseri

10.22034/JCHR.2024.1088022

Nanotechnology, the main technology in the twenty-first century, is the perception and control of matter at the dimensions 1-100 nanometers which revolutionized many aspects of modern life. Birds have not been used as commonly animal models in experimental toxicology bu More

Nanotechnology, the main technology in the twenty-first century, is the perception and control of matter at the dimensions 1-100 nanometers which revolutionized many aspects of modern life. Birds have not been used as commonly animal models in experimental toxicology but have proven useful in monitoring of environmental quality. This study aimed to evaluate zinc oxide nanoparticle (nano-ZnO) toxicity on kidneys in the homing pigeons (Columba livia f. urbana). The experimental groups orally received doses of 0, 30, 50, and 75 mg kg-1 b.w. of nano-ZnO (1 ml per bird, everyday) for time periods of 7 and 14 days. The lipid peroxidation (MDA/LPO) content, catalase (CAT) activity, and carbonyl protein (CP) content were increased and the total antioxidant capacity (TAC) level was lowered in kidney samples. The plasma levels of uric acid, urea, and creatinine were also slightly elevated (p>0.05). Histopathological examinations showed glomerular nanoparticle aggregation and tubular necrosis lines in the kidney parenchyma. In brief, nano-ZnO affected kidney function and structure in homing pigeons through the induction of oxidative stress. Manuscript profile

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78 - The Oxidative Stress State in Diabetes Mellitus Type 2 Patients with Different Medications Types
Israa Harjan Mohsen Mohammed Abed Jawad Abed J. Kadhim Mona N. Al-Terehi

10.22034/jchr.2022.690774

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79 - Evaluation of the Effect of Sedar (Ziziphus spina-christi) Extract on Biochemical Parameters of Skin Lesion in NMRI Mice
Shokooh Sharbatdar Nasim Hayati Roodbari Parichehr Yaghmaee

10.22034/ascij.2022.1965430.1416

The skin is one of the largest organs of the body and acts as a protective barrier against external harmful factors including microorganisms. It can be damaged and sometimes if the damage is too much, the hypoderm is also involved, so more time is needed for restoration More

The skin is one of the largest organs of the body and acts as a protective barrier against external harmful factors including microorganisms. It can be damaged and sometimes if the damage is too much, the hypoderm is also involved, so more time is needed for restoration. The purpose of this research is to investigate cedar plant on skin wound healing in NMRI laboratory mice. 24 mice with a weight of approximately 25-35 grams were bought and after wounding the skin on days 3, 6, 12, 21 they were treated with ointment prepared from cedar and oserin. Mice were randomly divided into three groups: sham, experimental 1, experimental 2. These groups were treated with oserin and Cedar ointment with a dose of 2 mg and 4 mg respectively. Wounds were measured after 3 weeks and their tissue was evaluated by hematoxylin-eosin staining. Biochemical indices of SOD, MDA and TAC were also investigated. Data analysis was done by SPSS version 22 software and Tukey's test. The wound size in Cedar 1 and 2 groups had a significant decrease compared to oserin (p < 0.05). The concentration of MDA in cedar groups 1 and 2 had a significant decrease compared to oserin (p < 0.05). In addition, the activity of SOD and TAC were significantly increased in cedar groups 1 and 2 compared to oserin (p < 0.05). Also, there was a significant increase in the thickness of epidermis and dermis in cedar 1 and 2 compared to oserin (p < 0.05). There was a significant increase in the number of fibroblasts and blood vessels in cedar 1 and 2 compared to oserin (p < 0.05). According to the results of the present study, it can be concluded that cedar extract was effective in wound healing by increasing SOD, TAC and decreasing MDA. Manuscript profile

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80 - The Effects of Hydroalcoholic Extract of Salvia officinalis on Liver Tissue and Cu, Fe, Zn and Mg Elements in Male Rats
شهرزاد خاکپور مریم خسروی معصومه میرزائی محیا نجاری

The imbalance between the production of free radicals and antioxidant can be the cause of oxidative stress.Oxidative stress induces different diseases and tissue damages. Isoniazid has been used for the purpose of producing free radicals. In the present study, we invest More

The imbalance between the production of free radicals and antioxidant can be the cause of oxidative stress.Oxidative stress induces different diseases and tissue damages. Isoniazid has been used for the purpose of producing free radicals. In the present study, we investigated the effects of Salvia officinalis hydroalcoholic extract on blood elements such as Fe, Mg, Zn and Cu. In this study, 63 male rats were used and divided in 9 groups; in each group 7 rats were included. Isoniazid 50mg/kg was orally administered to rats continuously once per day for 28 days using a feeding atraumatic needle. The experimental groups received for 28 days,Salvia officinalis hydroalcoholic extract with the doses 100, 250 and 400 mg/kg intraperitoneally, and also some experimental groups were coadministered with different doses of the extract and isoniazid; the results of serum Fe, Mg, Zn and Cu were compared with the control and sham groups, as well. Also, the animal livers of all groups were separated and dissected for the histopathological studies. Zn and Cu were increased significantly in the treated-groups with Salvia officinalis, but the levels of Fe and Mg showed a significant decrease in the experimental groups treated with all the doses of Salvia officinalis hydroalcoholic extract. It seems that tannin and bitter compounds of Salvia officinalis hydroalcoholic extract are the main agents to alternate the blood element levels. Manuscript profile

Open Access Article

81 - The Effect of Magnesium Oxide Nanoparticles on Oxidative Stress in a Parkinson's Model of Male Rats
Hoda Ghorbani moghaddam Akram Eidi Pejman Mortazavi Shahrbanoo Oryan

10.22034/ascij.2022.1958259.1392

Since limited studies have evaluated the antioxidant effects of magnesium oxide nanoparticles on Parkinson's disease, the aim of this study is to investigate the effect of magnesium oxide nanoparticles (MON) on oxidative stress in the Parkinson's model in mice. In this More

Since limited studies have evaluated the antioxidant effects of magnesium oxide nanoparticles on Parkinson's disease, the aim of this study is to investigate the effect of magnesium oxide nanoparticles (MON) on oxidative stress in the Parkinson's model in mice. In this experimental study, 54 adult male rats were divided into nine groups of six, including: healthy control group, parkinsonian control group receiving 6-hydroxydopamine in the lateral ventricle, sham group receiving normal saline and healthy experimental group receiving magnesium oxide nanoparticles in doses of 2.5, 5 and 10 mg/kg and experimental Parkinson's groups that in addition to inducing Parkinson's, received magnesium oxide nanoparticles in doses of 2.5, 5 and 10 mg/kg. Administration of nanoparticles was intraperitoneal for 30 days. After that, oxidative stress parameters MDA, CAT and SOD were measured in the brain tissue. The results of the present study showed that treatment with magnesium oxide nanoparticles significantly reduced the amount of oxidative stress parameters in the brain tissue (p < 0.05). Magnesium oxide nanoparticle treatment in doses of 5 and 10 mg/kg decreased MDA in parkinsonian groups compared to parkinsonian control animals. Also, the treatment of magnesium oxide nanoparticles in doses of 5 and 10 mg/kg in the parkinsonian group caused a significant increase in the activity of SOD and CAT enzymes compared to parkinsonian control animals. As a result, it can be said that magnesium oxide nanoparticle can play a promising role with its effectiveness in reducing oxidative stress processes in Parkinson's model. Manuscript profile

Open Access Article

82 - The Effects of Symbiotic on Oxidative Stress Reduction in Broilers with Pulmonary Hypertension Syndrome
Hamed Zarei Abbass Fereydoon Kolahi

Pulmonary Hypertension Syndrome (PHS), characterized by increased lung pressure in arteries, dilatation, and hypertrophy of the right ventricle, is correlated with the high cardiac output and enhanced blood pressure in the lungs to more blood oxygenation, which ultimate More

Pulmonary Hypertension Syndrome (PHS), characterized by increased lung pressure in arteries, dilatation, and hypertrophy of the right ventricle, is correlated with the high cardiac output and enhanced blood pressure in the lungs to more blood oxygenation, which ultimately leads to ascites. The accumulation of free radicals and increased oxidative stress result in both lipid peroxidation, as well as the increased levels of heterophil to lymphocytes. By having antioxidant properties, the synbiotic can significantly reduce oxidative stress. Therefore, in the present study, we aimed to investigate the therapeutic effects of synbiotic on pulmonary hypertension in broilers. Groups were categorized into; Sham (broilers with a basic diet in the whole period of the test), Control (recipient of basic diet+ triiodothyronine hormone (T3) at 1.5 mg/kg doses added to their diet from seven days of age), Treatment with synbiotic (birds with basic diet + T3 at 1.5 mg/kg dose added to their diet from seven days of age + synbiotic (0.1% dose) from day one). At 35 and 49 days of age, 9 birds from each group (3 broilers from each replicate) were randomly selected, and after getting the blood samples, heterophil to lymphocyte rate and lipid peroxidation was assessed. To evaluate pulmonary hypertension, cardiac weight index was also reported due to the right ventricular weight to the total weight of both ventricles (RV/TV). RV/TV rate at all ages was decreased in the synbiotic broilers; however, this was statistically significant at 49 days of age (p ≤ 0.05). Compared to the control group, lipid peroxidation levels in synbiotic group at all ages were reduced which was significant at 35 and 49 days of age (p < 0.05). Furthermore, Heterophil to lymphocytes rates was decreased in the synbiotic group compared to the control group in all ages, but this decrease was more significant at 49 days of age (p < 0.05). Regarding the obtained results, symbiotic, for their antioxidant properties, can significantly reduce the amount of oxidative stress result from pulmonary hypertension syndrome. Manuscript profile

Open Access Article

83 - The Evaluation of Serum Lipids and Oxidative Stress Indices Following Propofol Administration in Broilers
Maryam Karimi Dehkordi Majid Gholami-Ahangaran Pardis Banimehdi

The oxidative stress is one of the side effects of anesthesia. This study aimed to evaluate the changes in oxidative stress indices following propofol administration in broilers. For this purpose, 90 Ross broiler chicks were divided into two main and control groups. Ane More

The oxidative stress is one of the side effects of anesthesia. This study aimed to evaluate the changes in oxidative stress indices following propofol administration in broilers. For this purpose, 90 Ross broiler chicks were divided into two main and control groups. Anesthesia was induced by the injection of 2 mg propofol per kg of bird weight, intra-bone injection to induce anesthesia, and 0.5 mg propofol per kg bird weight to continuous anesthesia. To evaluate the serum lipids and oxidative stress indices (catalase activity, malondialdehyde concentration, and total antioxidant capacity), blood sampling was performed before anesthesia, during anesthesia, and 24 hours after the anesthesia. The results showed that the activity of serum catalase in 24 h after anesthesia (129.5 ± 6.69) was significantly higher than control chickens and its level during anesthesia (112.9 ±5.04), and before anesthesia (120.9 ± 4.21). The malondialdehyde concentration did not increase during anesthesia (34.3 ± 1.54), whereas it significantly increased 24 h after anesthesia (42.8 ± 4.28). Total antioxidant capacity significantly decreased 24 h after anesthesia (19.2 ± 1.67) compared to before (25.9 ± 1.27) and during anesthesia (27.4 ± 1.37). Total antioxidant capacity significantly increased before and during anesthesia compared to 24 h after anesthesia. Serum lipid indices did not change during the experiment. In general, this study's results showed that although using propofol in broiler chickens during anesthesia can effectively neutralize free radicals and prevent the side effects of oxidative stress, after anesthesia, oxidative stress indices increase. Manuscript profile

Open Access Article

84 - The Effect of Short-term Spirulina Supplementation on Oxidative Stress Status in Obese Men Following Resistance Exercise
Amin Rashid Lamir Javad Norouzi Babak Hooshmand moghadam

This study aimed to evaluate the effect of spirulina supplementation on oxidative stress markers (TAC and MDA) in the obese men after a resistance exercise session. In this study, 20 obese men were randomly divided into two groups of experimental (n = 10) and control (n More

This study aimed to evaluate the effect of spirulina supplementation on oxidative stress markers (TAC and MDA) in the obese men after a resistance exercise session. In this study, 20 obese men were randomly divided into two groups of experimental (n = 10) and control (n = 10). The experimental group received 2 spirulina tablets (500 mg) daily for two weeks, and the control group received the same amount of placebo. After two weeks of the supplementation, the individuals from both groups participated in a resistance exercise session. Blood samples were collected in three steps; before supplementation, before, and 24 hours after resistance exercise. Results showed that the TAC and MDA concentration of the biomarker in the experimental group were respectively increased and decreased significantly in the control group over time (p ≤ 0.05). This study showed that the supplementation with Spirulina could increase the plasma antioxidant capacity which reduced the lipid peroxidation in obese men after the resistance exercise. Manuscript profile

Open Access Article

85 - The Effect of Herniarin on Oxidative Stress in the Hippocampus in Streptozotocin-induced Diabetic Rats
Zahra Shaibani

10.22034/ascij.2021.684776

Diabetes increases the risk of central nervous system disorders such as stroke, seizures, dementia, and cognitive impairment. The herniarin has a phenolic compound and it is a powerful antioxidant whose efficacy has been reported in neurodegenerative disorders in recent More

Diabetes increases the risk of central nervous system disorders such as stroke, seizures, dementia, and cognitive impairment. The herniarin has a phenolic compound and it is a powerful antioxidant whose efficacy has been reported in neurodegenerative disorders in recent studies. Moreover, it has been shown that hyperglycemia induces spontaneous oxidation of glucose through enzymatic and non-enzymatic processes, leading to oxidative stress by stimulating the production of active oxygen and nitrogen components. In this study, 40 male Wistar rats were randomly selected and divided into four groups: control, diabetic, and diabetic treated with 150 and 300 mg/kg hernia. Diabetes was induced by intraperitoneal injection of streptozotocin (STZ) at a dose of 60 mg/kg. One week after streptozotocin injection, treatment with herniarin at 300 and 150 mg/kg was performed orally for two weeks. Blood glucose was measured after STZ injection by blood sampling from the caudal vein. Lipid peroxidation, thiol levels, and glutathione peroxidase activity were measured as indicators of oxidative stress in the hippocampus. Finally, the data of the groups were analyzed using SPSS statistical software and one-way ANOVA and Tukey tests. After induction of diabetes, an increase in lipid peroxidation, a decrease in thiol, and a decrease in glutathione peroxidase activity was observed in the hippocampus of diabetic rats compared to the control group (p < 0.001). Daily doses of 150 and 300 mg/kg Herniarin improved oxidative stress in the brains of diabetic rats. The present study showed that treatment with herniarin significantly reduced oxidative stress in the hippocampus of STZ diabetic rats. Manuscript profile

Open Access Article

86 - Evaluation of the Effect of Ferula gummosa Extract on Biochemical Parameters of a Skin Wound in NMRI Mice
Parisa Seifali Parichehreh Yaghmaei Nasim Hayati Roodbari

10.22034/ascij.2022.1964577.1414

Wound healing is one of the important problems in medicine. Therefore, this study aimed to investigate the effect of Ferula gummosa extract on skin wound healing in NMRI mice. 24 NMRI mice with skin ulcers, were treated with Ferula gummosa ointment on days 3, 6,12, and More

Wound healing is one of the important problems in medicine. Therefore, this study aimed to investigate the effect of Ferula gummosa extract on skin wound healing in NMRI mice. 24 NMRI mice with skin ulcers, were treated with Ferula gummosa ointment on days 3, 6,12, and 21, and randomly divided into three eucerin groups (daily recipient eucerin ointment), the experimental group1(daily treatment with Ferula gummosa ointment at a dose of 2 mg) and the experimental group 2 (daily treatment with Ferula gummosa ointment at a dose of 4 mg and the ulcers were measured with a calliper and the biochemical indexes of superoxide dismutase (SOD), malondialdehyde (MDA) and total antioxidant capacity (TAC) in tissue, were measured. Then, tissue sections were prepared from the wound and evaluated histopathologically by hematoxylin-eosin staining. The data were analyzed by SPSS 22 software and the Tukey test at a significance level of p < 0.05 and the histograms were drawn in excel. wound size in the Ferula gummosa groups compared to the Eucerin group showed a significant decrease (p < 0.05). The concentration of MDA in groups the Ferula gummosa significantly decreased time-dependent compared with the eucerin group (p < 0.05). SOD activity and TAC concentration also showed a significant increase in time-dependent in groups Ferula gummosa compared to the eucerin group (p < 0.05). Based on histopathological findings, epidermal and dermal thickness increased significantly in groups Barijeh compared to eucerin group (p < 0.05), and the number of fibroblasts and blood vessels in the two groups of Barijeh in a time-dependent manner compared to the Eucerin group showed a significant increase (p < 0.05). According to the results, it was shown that Ferula gummosa is effective in wound healing and repair by increasing SOD and TAC activity and decreasing MDA concentration, as well as histopathological results. Therefore, the findings of this study showed a new perspective on this herbal medicine for us wound healing. . Manuscript profile

Open Access Article

87 - Antioxidant Effects of 6-gingerol on Serum Levels of Liver Enzymes and Oxidative Stress-induced Markers with Gold Nanoparticles in Rat Liver Tissue
BibiFatemeh Fatemi Gholamhassan Vaezi Shahram Sharafi Raheleh Rahbarian

10.22034/ascij.2021.687845

Gold nanoparticles disturb the physiological processes of the liver tissue by affecting the body’s biological system. Given gingerol's antioxidant properties, researchers wanted to see how it affected the levels of liver enzymes and oxidative stress indices in the More

Gold nanoparticles disturb the physiological processes of the liver tissue by affecting the body’s biological system. Given gingerol's antioxidant properties, researchers wanted to see how it affected the levels of liver enzymes and oxidative stress indices in the liver tissue of rats exposed to gold nanoparticles.This experimental research was conducted on 32 male Wistar rats, divided into four equal groups of control, gold nanoparticle (one administration of 0.5 ml gold nanoparticle, 200 ppm and 60 nanometers), gold nanoparticles + 50 mg/kg concentration of gingerol, and gold nanoparticles + 100 mg/kg gingerol. At the end of treatment period, the serum level of ALT, AST, GGT, ALP, and level of antioxidant enzymes of SOD < GST, and CAT, and levels of MDA and HOdG-8 in the liver tissue were measured using the ELISA method. In this study, rats receiving gold nanoparticles with concentrations of 50 and 100 mg/kg gingerol had significantly higher levels of SOD, GST, and CAT enzymes in their liver tissue than the dose-dependent group receiving gold nanoparticles. The serum levels of ALT, AST, GGT, and ALP, on the other hand, were reduced. Furthermore, the levels of MDA and HOdG-8 in rat liver tissue were found to be significantly lower (P <0.001).According to the results of study, gingerol improved the oxidative stress indices in liver tissue and decreased the serum level of liver enzymes. Therefore, this compound can be exploited to reduce DNA oxidative stress and lipid peroxidation in liver tissue. Manuscript profile

Open Access Article

88 - The Effect of Ibuprofen on Sperm Parameters, Oxidative Stress and Histology of Mice Testis
Safoura Shafigh Jazi Niloofar Sadeghi Dina Zohrabi Marzieh Tavalaee Mohammad Hossein Nasr-Esfahani

10.22034/ascij.2022.1940492.1306

Recently, the application of drugs administered for reasons other than male infertility may have profound effect on fertility potential. Ibuprofen, as one of the non-steroidal anti-inflammatory drugs can possibly have a negative effect on the spermatogenesis process whi More

Recently, the application of drugs administered for reasons other than male infertility may have profound effect on fertility potential. Ibuprofen, as one of the non-steroidal anti-inflammatory drugs can possibly have a negative effect on the spermatogenesis process which could be dose-dependent. This side effect is likely acquired through oxidative stress. Therefore, in this study, we assessed the effect of different doses of Ibuprofen on spermatogenesis, sperm parameters, and oxidative stress. In this study, 15 male NMRI mice were daily gavaged by different doses of Ibuprofen (0, 5, 25, 50, and 100 mg/kg) for one month. Then, morphometric parameters of testis, sperm concentration, motility and oxidative stress were assessed. Finally, the data were analyzed using SPSS software and ANOVA test. Different doses of Ibuprofen had no effect on Johnson score and sperm concentration as compared to the control group. However, sperm motility was insignificantly reduced only at a dose of 100 mg / kg. In addition, the level of oxidative stress in all doses of Ibuprofen increased compared to the control group and, this increase was only significant at the dose of 100 mg/kg (P=0.03). Based on the results of this study, continuous administration of Ibuprofen for one month has no significant effect on spermatogenesis and sperm parameters but significantly increase ROS production, the effect of which on sperm chromatin integrity, for longer period remain a concern, which needs more studies in the future.   Manuscript profile

Open Access Article

89 - The Antioxidant Effect of N-Acetylcysteine on the Expression of SOD and HOG Genes in Human Dermal Fibroblast in High Glucose State
Bahareh Safavi Zeinab Piravar Mina Ramezani

10.22034/ascij.2022.1949310.1353

Wound healing in diabetic patients is delayed, because of oxidative stress. This study aims at investigating the molecular changes in Human Dermal Fibroblasts (HDFs) in a high-glucose state and improving the effect of N-Acetyl-L-Cysteine (NAC) and gene expression of oxi More

Wound healing in diabetic patients is delayed, because of oxidative stress. This study aims at investigating the molecular changes in Human Dermal Fibroblasts (HDFs) in a high-glucose state and improving the effect of N-Acetyl-L-Cysteine (NAC) and gene expression of oxidative genes Super-Oxide Dismutase (SOD) and Heme Oxygenase1 (HO1). HDFs were cultured in 5.5, 25, 50, and 75 mM glucose concentrations for 72 hours. Cell proliferation was examined via 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) assays. Oxidative stress markers of SOD and HO1 were quantified with real-time Polymerase Chain Reaction (PCR). The antioxidant effect of NAC on 1 mM was examined to evaluate oxidative markers in the glucose effects on the HDFs. The MTT assay revealed a decline in cell viability in 50 and 75 mM glucose concentrations. mRNA level of SOD and HO1 was upregulated. The antioxidant addition of NAC reduced the inhibitory effect of the high-glucose state on the proliferation of the HDFs. A high-glucose state impairs the in vitro proliferation and migration of HDFs and may, therefore, induce increased oxidative stress and cellular dysfunction. The antioxidant effect of NAC ameliorates the damaging impact of a high-glucose state. Manuscript profile

Open Access Article

90 - The Effect of Oleuropein on Chronic Stress-induced Anxiety: Possible Role of Oxidative Stress and Serum Corticosterone
Maede Jafari Zahra Hushmandi Gelavij Mahmoodi

10.22034/ascij.2022.1936837.1281

Chronic stress is associated with the pathophysiology of various psychiatric disorders, including anxiety disorders and depression. The aim of the present study was to evaluate the effectiveness of oleuropein on anxiety in mice under chronic stress. In order to induce c More

Chronic stress is associated with the pathophysiology of various psychiatric disorders, including anxiety disorders and depression. The aim of the present study was to evaluate the effectiveness of oleuropein on anxiety in mice under chronic stress. In order to induce chronic stress (CRS), rats were subjected to immobility stress for 2 hours daily in the Restrainer device for two weeks and then to electric shock with a power of half milliampere for two minutes. Rats under chronic stress were divided into five groups, including control (receiver of drug solvent), treatment (oleuropein at doses of 7.5, 15, 30 mg/kg) and positive control (diazepam). In CRS rats, oleuropein at a dose of 30 mg/kg increased the number of entries and the time spent in the open arms of the EPM (p < 0.05). The administration of oleuropein in CRS rats at a dose of 7.5 mg/kg decreased the frequency of being on the edge in the open page test (p < 0.05). Administering different doses of oleuropein decreased lipid peroxidation and increased antioxidant capacity of brain and serum in CRS rats (p < 0.05). Oleuropein also decreased serum corticosterone in CRS rats. The effectiveness of oleuropein on anxiety behaviors in mice under chronic stress is through reducing oxidative stress. . Manuscript profile

Open Access Article

91 - Investigating the Different Antioxidant Effect of Turmeric and Melatonin in Three Tissues of Brain, Liver and Kidney under the Conditions of Social Stress
Irandokht Zeynaei Shahrbanoo Oryan Mohammadreza Vaez Mahdavi Akram Eidi

10.22034/ascij.2023.1964029.1406

The purpose of this research is to investigate the antioxidant effect of turmeric and melatonin in three tissues of brain, liver and kidney under the same stressful conditions.For this purpose, 40 male Wistar rats were kept in different conditions to induce the desired More

The purpose of this research is to investigate the antioxidant effect of turmeric and melatonin in three tissues of brain, liver and kidney under the same stressful conditions.For this purpose, 40 male Wistar rats were kept in different conditions to induce the desired stresses.Stresses include: food restriction, change of roommate and observation in conditions of having and not having turmeric and melatonin antioxidants.The rats were kept for 10 weeks in the defined conditions for each group, and after the completion of the period, the level of glutathione present in the homogenate of the brain, liver and kidney tissues was evaluated.At first, to ensure the induction of stress, the amount of glutathione and malondialdehyde was measured and compared to the control group.The result indicated the increase of glutathione and malondialdehyde and in fact the induction of stress.The general result indicates the effectiveness and protective effect of turmeric and melatonin in inhibiting oxidative stress in all three mentioned tissues, but this effectiveness is not seen in all three tissues with the same intensity.Contrary to the fact that turmeric was able to reduce the level of malondialdehyde and prove its antioxidant role in all three tissues of the brain, liver and kidney, it could not show its antioxidant role well in the liver tissue and the amount of glutathione did not decrease.Unlike turmeric, melatonin was able to show its antioxidant role in liver tissue cells better than in brain and kidney tissue, so that it was able to reduce the level of glutathione in these two tissues.Therefore, antioxidants probably have different functions in different tissues. . Manuscript profile

Open Access Article

92 - The Effect of Intraperitoneal Injection of Carvacrol Administration on Lipopolysaccharides-Induced Renal Oxidative Stress in Male Rats
Alireza Mortazavi Hossain Mohammad Pourkargar Farimah Beheshti Gholamhasan Vaezi Mahmoud Hosseini

10.22034/ascij.2023.1978481.1461

Carvacrol is a natural monoterpene phenol which retains significant antibacterial, antifungal, anti-cancer and anti-inflammatory activity. However, the effect of carvacrol on oxidative stress in lipopolysaccharide-induced acute kidney injury has not yet been reported. T More

Carvacrol is a natural monoterpene phenol which retains significant antibacterial, antifungal, anti-cancer and anti-inflammatory activity. However, the effect of carvacrol on oxidative stress in lipopolysaccharide-induced acute kidney injury has not yet been reported. The present study aimed to investigate the protective effects of carvacrol on lipopolysaccharides-induced renal oxidative stress and inflammatory responses in male rats. Male Wistar rats (weighing 200 to 250 g, n=7 each group) were divided into the following groups: 1) control, 2) LPS, 3) LPS+carvacrol 25 mg/kg, 4) LPS+carvacrol 50 mg/kg and 5) LPS+carvacrol 100 mg/kg. For inducing acute kidney injury, 1 mg/kg LPS was injected intraperitoneally for 2 weeks. Carvacrol was administered intraperitoneally 30 minutes prior LPS injection. After preparation of renal homogenates, levels of inflammatory cytokines IL-1β and oxidative stress indexes (malondialdehyde, total thiol, catalase and Superoxide dismutase activity) were detected by ELISA and spectrophotometric methods, respectively. Our results showed that LPS injection increased malondialdehyde and IL-1β while, it reduced total thiol, catalase and SOD activity in the renal tissue. But, pretreatment with carvacrol not only decreased malondialdehyde and IL-1β levels but also increased total thiol, catalase and SOD activity. Simple regression analysis revealed that MDA was positively correlated with IL-1β level). However, there was a significant negative correlation between MDA level and total thiol, SOD and CAT activities. Our results showed that carvacrol protects the kidney by reducing IL-1β and preventing oxidative stress in the renal tissue.enal tissue. Manuscript profile

Open Access Article

93 - Protective Effect of Coenzyme Q10 on Rat Sperm and Testicular Tissue after Exposure to Diazinon
Sima Ebadi Naftchali Ramezan Khanbabaei Abasali Dehpour Jouybari Roya Bishekolaei Esmaeil Fattahi

10.22034/ascij.2023.1991460.1512

Diazinon (DZN) is an organophosphate insecticide that causes a wide range of pathological effects on the male reproductive system, disturbances in sperm production and quality, and fertility problems. This study investigated the protective effect of coenzyme Q10 treatme More

Diazinon (DZN) is an organophosphate insecticide that causes a wide range of pathological effects on the male reproductive system, disturbances in sperm production and quality, and fertility problems. This study investigated the protective effect of coenzyme Q10 treatment on sperm quality, oxidant/antioxidant system, and histopathology of testicular tissue in male rats after exposure to diazinon. This experimental study was carried out on 16 adult male Wistar albino rats with an approximate weight of 150 to 230 gr, which were obtained from the animal care center.The animals were randomly divided into four groups (n=4) and were tested by intraperitoneal injection for 30 days which including the diazinon exposure group (50 mg/kg of diazinon dissolved in sesame oil intraperitoneally injected), the coenzyme Q10 and diazinon exposure group (50 mg/kg of diazinon and 10 mg/kg of coenzyme Q10 dissolved in sesame oil intraperitoneally injected), group of coenzyme Q10 (10 mg/kg of coenzyme Q10 dissolved in sesame oil intraperitoneally injected), and the control group (1.5 mg/kg of sesame oil intraperitoneally injected). After treating coenzyme Q10 with diazinon, a significant increase in the number and motility of sperm and a decrease in sperm mortality were observed (P<0/05). Exposure to diazinon with coenzyme Q10 treatment was able to decrease the level of malondialdehyde (MDA) and increase the activity of superoxide dismutase (SOD) compared to the diazinon group (P<0/05). Simultaneous exposure to diazinon and coenzyme Q10 treatment led to a decrease in testicular histopathological damage after exposure to diazinon. It seems that coenzyme Q10 can have a good protective effect against oxidative damage in the male reproductive system caused by exposure to diazinon. Manuscript profile

Open Access Article

94 - Evaluating the Effect of Coenzyme Q10 (CoQ10) on Fertility, Depression, and Anxiety in Male Rats
Maryam Sharafi chie Ramin Hajikhani Jalal Solati Maryam khosravi

Quenzim Q10 (CoQ10) is a benzoquinone compound soluble in androgen lipid found in most tissues of the body. CoQ10 has a potent antioxidant activity and neutralizes free radicals. The aim of this study was to investigate the effect of CoQ10 as a potent antioxidant and fr More

Quenzim Q10 (CoQ10) is a benzoquinone compound soluble in androgen lipid found in most tissues of the body. CoQ10 has a potent antioxidant activity and neutralizes free radicals. The aim of this study was to investigate the effect of CoQ10 as a potent antioxidant and free radicals neutralizer on reproductive disorders, sexual behaviors, anxiety, and depression in male rats. In this study, small male rats (weight range of30-35 g) were used. At first, the animals under study passed a two-week treatment period. The adult male rats were treated with doses of 200, 300, 400 mg/kg of CoQ10 for two weeks. Then the effects of the given treatment were studied in the physiologic section. After the end of the treatment period, the sexual behaviors were assessed in the control group and treated groups by sexual behavior test. Then, the anxiety and depression levels in adult male rats were assessed using EPM and forced swimming tests, respectively. The findings of this study showed that the prescription of CoQ10 alone did not have a significant effect on sexual behaviors, depression, and anxiety in male rats. Manuscript profile

Open Access Article

95 - Effect of Subacute Toxicity Nano Zinc Oxide (ZnO NPs) on Oxidative Stress Enzymes of Roach (Rutilus rutilus caspicus)
K. Karimzadeh A. Zahmatkesh E. Sharifi

Today, the excessive use of zinc oxide nanoparticles (ZnO NPs) has led to concerns about the potential environmental hazards caused by the presence of these particles in aquatic ecosystems. Therefore, the aim of this study was to investigate the toxic effects of zinc ox More

Today, the excessive use of zinc oxide nanoparticles (ZnO NPs) has led to concerns about the potential environmental hazards caused by the presence of these particles in aquatic ecosystems. Therefore, the aim of this study was to investigate the toxic effects of zinc oxide nanoparticles (0.02, 0.05 and 0.1 mg/L) on the oxidative stress enzymes in brain tissue of roach during a period of 7 days. After homogenization of the brain tissue, the activity of oxidative stress enzymes such as superoxide dismutase (SOD), glutathione-S transferase (GST), catalase (CAT), glutathione (GSH) and malondialdehyde (MDA) was determined using biochemical methods. The SOD, CAT and GST activities were significantly increased by exposure to 0.1 mg/ml zinc nanoparticles compared with other concentrations in brain tissue of roach (P<0.05). However, the amount of glutathione decreased with increasing exposure dose. In amount of malondialdehyde dose-dependent manner was observed, since the maximum concentration was recorded at 0.1 mg/ml of nanoparticles (4.5 ± 5.3 nmol/g wet). The subacute toxicity of nanoparticles leads to the induction of free radical and oxidative stress in brain tissue of roach. The increase in the activity of antioxidant enzymes causes antioxidant defense system activation for scavenger in free radicals. Manuscript profile

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96 - The Effect of Skullcap Supplementation after a Session of Simulated Karate Activity on Some Antioxidant Indicators, Oxidative Stress and Selected Muscle Damage in Elite Karate Athletes
Mohammad Reza Fazli Reza Gharakhanlou Mohammad Shariatzadeh Joneydi

Karate is considered as one of the most intense and challenging exercise due to its intensity and types of activities. In these activities, the body is inflicted by the number of injuries and its antioxidant capacity is disturbed. Therefore, the present study was aimed More

Karate is considered as one of the most intense and challenging exercise due to its intensity and types of activities. In these activities, the body is inflicted by the number of injuries and its antioxidant capacity is disturbed. Therefore, the present study was aimed at assessing the responses of muscle damage markers, oxidative stress and plasma antioxidant enzymes to the simulated karate activities and supplementation of skullcap plant in the elite karate athletes. Twenty-four eligible elite karate athletes were randomly assigned to two groups, consisting of placebo-activity group (n=12) and skullcap-activity group (n=12). The considered activities were based on the World and European Karate Championships programs. First, blood samples were taken from research groups at the rest and then desired activities were performed by athletes. Next, the second blood sampling was taken, immediately after the activity and after that, the subjects in placebo and skullcap groups received 500 mg of placebo and skullcap capsules, respectively. The third blood samples were taken one hour after that and the fourth blood sampling was two hours after taking the supplement. Finally, plasma was removed from the blood and CK, LDH, MDA, GPx, TAC, and SOD indicators were measured.Results showed that the indicators of muscle damage and oxidative stress significantly increased (p < 0.001) immediately after simulated karate activates and decreased during the recovery period as well, while these changes were higher in supplement group (p < 0.05). Furthermore, antioxidant indicators have significantly increased after the activities, and this increase was at a higher level (p < 0.05) throughout the recovery period in the supplement group. The results of the present study showed that skullcap supplementation increased antioxidant indicators in the recovery period and decreased muscle and oxidative damage factors. Therefore, this plant can be recommended as a supplement to reduce damages, stress, and fatigue for karate athletes. Manuscript profile

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97 - Oxidative Stress Enzymes and Liver Tissue Changes of Male Rats under the Influence of Dust in Different Cities of Khuzestan Province
Najmeh Soleimani Mohammad Reza Dayer Tayebeh Mohammadi Hassan Faridnouri

The city of Ahvaz is considered the most polluted city in the world by blowing dust from neighboring countries which makes this study important. The results show that the smaller the particles, the more permeable they become and pass through the respiratory filters and More

The city of Ahvaz is considered the most polluted city in the world by blowing dust from neighboring countries which makes this study important. The results show that the smaller the particles, the more permeable they become and pass through the respiratory filters and causing disease. In this study, the effect of fine particulate matters in different cities of Khuzestan province of Iran was compared on the liver histology and oxidative stress enzymes of male rats. 30 adult male rats were divided into six equal groups including control, Ahvaz, Ramhormoz, Andimeshk, Abadan, and Susangard which were treated for two months. The results of serum oxidative stress enzyme activity increased only for the glutathione peroxidase in Ramhormoz and Abadan groups. The histological examination of liver revealed bleeding, mild hyperemia, and changes in hepatocyte cells. The nuclear area in central vein region, hepatocyte area in portal, and vein regions showed significant changes. Moreover, kupffer cell number of the portal region significantly increased in Ahvaz, Abadan, and Andimeshk groups. Therefore, due to different heavy metals composition, fine dusts have different effects on blood and liver parameters which depend on their amount and duration of exposure.   Manuscript profile

Open Access Article

98 - The Health-Related Biological Effects of Date Palm Pollen (DPP) along with Physical Activity: A narrative review
Soheil Abdollahi mohammad ali azarbayjani Maghsoud Peeri saleh Rahmati-Ahmadabad

Date palm pollen (DDP), which is found in the male date tree, actually fertilizes the female date tree, which is necessary for the growth of date fruit. It has long been used in traditional medicine as a medicinal plant with fertility effects and increased libido. The i More

Date palm pollen (DDP), which is found in the male date tree, actually fertilizes the female date tree, which is necessary for the growth of date fruit. It has long been used in traditional medicine as a medicinal plant with fertility effects and increased libido. The information obtained from the studies shows that this pollen, due to its antioxidant and gonadotropin-stimulating compounds, can increase fertility and sexual potency in both women and men with two main mechanisms. The first mechanism is this effect on the stimulation of gonadotrophic hormones and the second is a reduction of oxidative stress in the testicular tissue and consequently a protective effect on the testicular tissue. Due to the presence of antioxidant compounds, the consumption of this pollen also applies its health-enhancing effects to other tissues such as the liver, intestines, and heart. In recent years, researchers have paid attention to this pollen as an important factor to strengthen the health-enhancing effects of physical activity (PA), and researchers have investigated the simultaneous effect of this pollen and PA. Despite the few studies done, it seems that this pollen can enhance the antioxidant and anti-inflammatory effects of regular PAs. Nevertheless, it seems necessary to further studies in this area. Manuscript profile

Open Access Article

99 - The effect of salicylic and jasmonic acid on antioxidant enzymes activity, soluble carbohydrate and proline in Artichoke (Cynara Scolymus L.) under salt stress conditions
S.N Seyedalikhani A.R Pazoki

In order to investigate The effect of salicylic and jasmonic acid on antioxidant enzymesactivity, soluble carbohydrate and proline in artichoke (Cynara Scolymus L.) under salt stress conditions, a research was conducted in 2014 at Islamic Azad University, Yadgar-e-Imam More

In order to investigate The effect of salicylic and jasmonic acid on antioxidant enzymesactivity, soluble carbohydrate and proline in artichoke (Cynara Scolymus L.) under salt stress conditions, a research was conducted in 2014 at Islamic Azad University, Yadgar-e-Imam (RAH) Shahre Rey Branch. The experiment was done as factorial based on completely random design with four replications, the first factor salinity stress in four levels 0, 25, 60, and 95 mM, the second factor in two levels no consumption and consumption of 0.7 mM salicylic acid and the third factor no consumption and consumption of 100 µm jasmonic acid were considered. The results showed that all three main effects of salinity stress, salicylic acid and jasmonic acid consumption on antioxidant enzymes activity, soluble carbohydrate and proline content were significant at the 1% probability level, so in salinity stress conditions, the enzyme activity showed a significant increase and the highest level of activity was gained in 95 mM sodium chloride and the lowest one was related to the non applying of salt stress. Also, under the conditions of salicylic acid and jasmonic acid foliar application, the activity level of antioxidant enzymes and soluble carbohydrate and proline content increased and as the result the resistance of artichoke resistance to salt stress improved. Manuscript profile

Open Access Article

100 - Determination of oxidative stress indicator in dairy cattle with subclinical mastitis
ام البنین GHasemian karik SH Safi

  The most important and common disease among dairy cows is mastitis and it is still a big challenge for the dairy industry all over the world.Oxidative stress induced by reactive oxygen species(ROS) is a major cause of different diseases especially mastitis in ca More

  The most important and common disease among dairy cows is mastitis and it is still a big challenge for the dairy industry all over the world.Oxidative stress induced by reactive oxygen species(ROS) is a major cause of different diseases especially mastitis in cattle.there are few studies on the role of ROS in occurrence of subclinical mastitis.the objective of the persent study was to evaluate the changes in plasma malondialdehyde concentrations as a marker of lipid peroxidation of red blood cells and its relationship with somatic cell count(SCC) in milk as the marker of mammary inflammation in cattle.Heparinized blood and milk samples were collected from 45 healthy cows and 45 cows with subclinical mastitis from dairy farms in Tehran province,Iran.No significant difference (p>0.05) was seen between concentrations of malondialdehyde in the studied groups.there was a significant difference (p<0.001) between SCCs in the healthy cows with subclinical mastitis.the concentration of plasma malondialdehyde was higher in cows with subclinical mastitis which indicates mild increase in production of ROS in response to invading pathogens.     Manuscript profile

Open Access Article

101 - Protective Effect of Coenzyme Q10 and Vitamin C on Cysteamine�Induced Lipid Peroxidation
سروش Almasi بابک Rezvanjoo S.H Shirazibeheshtiha علی Namvaran AbbasAbad مهدی khosravi

Benefts of antioxidant supplementation in various disorders through reducing free radicals and improving organsperformance have been reported. The aim of this study was to investigate the effects of coenzyme Q10 and vitaminC on Cysteamine-induced lipid peroxidation and More

Benefts of antioxidant supplementation in various disorders through reducing free radicals and improving organsperformance have been reported. The aim of this study was to investigate the effects of coenzyme Q10 and vitaminC on Cysteamine-induced lipid peroxidation and oxidative stress.This experimental interventional study was conducted on male Wistar rats. Animals were divided into four groups(six rats) randomly. Groups were treated as; group 1 (Normal saline), Group 2 (Cysteamine), Group 3 (vitaminC plus Cysteamine), Group 4 (coenzyme Q10 plus Cysteamine). 24 hours after the last injection, rats wereanesthetized and sampled for investigations. Welch’s and Kruskal-Wallis tests were used for analyzing data and P< 0.05 was set the signifcance level.The results of this study indicate that injection of cysteamine signifcantly (P < 0.05) decreased glutathioneperoxidase activity compared with control group. Pretreatment with vitamin C signifcantly (P < 0.05) increasedglutathione peroxidase activity compared with cysteamine group. Pretreatment with coenzyme Q10 increasedglutathione peroxidase activity (P <0.001) and superoxide dismutase (P <0.05) signifcantly compared withcysteamine group.Based on the results of this study, coenzyme Q10 and vitamin C can be used in reducing oxidative stress inducedcysteamine. Manuscript profile

Open Access Article

102 - An investigation into the effect of Morus nigra L. leaf extract on Parkinson’s disease symptoms and oxidative stress markers in male rats
فریبرز Moayer آریا Badiei A.R SHaghayegh فرهاد Mosa khani

  Parkinson,s disease (PD) is a progressive neurodegenerative disorder.in this disease activity of dopaminergic neurons in the substantia nigra and dopamine level in the striatum.there is much evidence in effect bof oxidative stress as pathogen agents of parkinson More

  Parkinson,s disease (PD) is a progressive neurodegenerative disorder.in this disease activity of dopaminergic neurons in the substantia nigra and dopamine level in the striatum.there is much evidence in effect bof oxidative stress as pathogen agents of parkinson,s disease progression.angiotensin II activates NADPH depending oxidases and produce superoxides formation.morus nigar L.extract shows good angiotensin converting enzyme(ACE) inhibitory effects,in vitro.the aim of this study is to study the antioxidant effects of morus nigar L.leaf extract on parkinson,s disease symptoms and oxidative stress markers.48 male rats divided randomly into four groups(n=12 each):1) sham,(2) toxin, (3) captopril, (4) treatment.rate in sham group were used as normal controls.rats in toxin group were injected with 6-OHDA. captopril group were injected i.p. with captopril (5mg/kg) daily 6 days before and 4 &24 hrs after the injection of 6-OHDA and treatment group were injected i.p. with morus nigra L.(10mg/kg) extract daily 6 days before the injection of 6-OHDA in the substantia nigra and 4 &24 hrs after it.muscle stiffness,rotation test and histological test were assessed in 6 rats of any groups after 2 weeks.protein oxidation,lipid peroxidation and ACE activity were assessed in brain in 6 rats of any groups 24 hrs,after the 6-OHDA injection.the results suggested that the use of aqueous extract of morus nigra can be useful in reduction of parkinson,s disease symptoms and reduce oxidative stress markers and dopaminergic neuronal death.     Manuscript profile

Open Access Article

103 - immunohistochemical evaluation in testicular tissue of male rats following PM2.5 particle inhalation
elnaz noshadirad kazem parivar Saeed Motesaddi Zarandi Pejman Mortazavi batool Gorbani yekta

Background & Aim: Exposure to suspended particles (PM2.5) and gaseous air pollution poses a serious threat to spermatogenesis in men. However, the specific molecular mechanism behind this remains largely unknown. This study aims to investigate the impact of PM2.5 and ga More

Background & Aim: Exposure to suspended particles (PM2.5) and gaseous air pollution poses a serious threat to spermatogenesis in men. However, the specific molecular mechanism behind this remains largely unknown. This study aims to investigate the impact of PM2.5 and gas pollution on various aspects including the oxidant/antioxidant system, oxidative stress and also the changes in the expression level of aquaporin protein through immunohistochemistry and western blotting in testicular tissue were investigated. Materials & Methods: A total of 36 male Wistar rats were randomly divided into three groups: a control group exposed to standard air conditions, a group exposed to gas pollutants alone (Gas), and a group exposed to both gas pollutants and PM2.5 (Gas+PM2.5). All groups were subjected to pollutant exposure for three months, four days a week, and five hours a day. Results: The findings revealed that co-exposure to gaseous pollutants and PM2.5 led to increased concentration of Malondialdehyde (MDA), as well as decreased in aquaporin 9 protein in immunohistochemistry and western blot, compared to the control group. Conclusion: Therefore, exposure to PM2.5 and gaseous pollutants probably provokes oxidative stress in the testis, which leads to the reduction of aquaporin 9 protein through the activation of signaling pathways. Thus, PM2.5 pollution appears to play a crucial role in infertility by disrupting spermatogenesis. Manuscript profile

Open Access Article

104 - Effects of different concentrations of lead on some biochemical and physiological parameters of rice (Oryza sativa L.)
Javad Salvati Hormoz Fallah yousof niknejad Davood Barari Tari

Lead (Pb) not only negatively alters plant growth and yield but may also have potentially toxic risks to human health. In the present study, the effects of different concentrations of Pb (150 and 300 μM) on growth, physiological and biochemical attributes of rice under More

Lead (Pb) not only negatively alters plant growth and yield but may also have potentially toxic risks to human health. In the present study, the effects of different concentrations of Pb (150 and 300 μM) on growth, physiological and biochemical attributes of rice under hydroponic conditions were investigated. The results showed that Pb treatments with negative effects on chlorophyll metabolism reduced photosynthetic pigments and, consequently, diminished the growth and biomass of rice plants. Pb stress induced oxidative stress and damage to bio-membranes by increasing the accumulation of hydrogen peroxide and methylglyoxal. The activity of antioxidant enzymes and glyoxalase cycle was upregulated in rice leaves under Pb toxicity. Pb treatments altered the levels of non-enzymatic antioxidant compounds (glutathione (GSH) and ascorbic acid (ASA)) in the leaves of rice plants by reducing the ratios of reduced ASA to oxidized ASA and reduced GSH to oxidized GSH compared to control plants. Therefore, concentrations of 150 and 300 μM Pb in the soil can induce negative effects on important physiological and metabolic processes of rice, which reduce plant growth and biomass. Manuscript profile

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