The Effect of Ibuprofen on Sperm Parameters, Oxidative Stress and Histology of Mice Testis

Subject Areas : Journal of Animal Biology

Safoura Shafigh Jazi ¹ , Niloofar Sadeghi ² , Dina Zohrabi ³ , Marzieh Tavalaee ⁴ , Mohammad Hossein Nasr-Esfahani ⁵

1 - Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran. Department of Biology, Faculty of Science, NourDanesh Institute of Higher Education, Isfahan, Iran.
2 - Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
3 - Department of Biology, Faculty of Science, NourDanesh Institute of Higher Education, Isfahan, Iran
4 - Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
5 - Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran

Received: 2021-05-15 Accepted : 2021-09-11 Published : 2022-05-22

Keywords: Oxidative stress, Ibuprofen, Sperm parameters,

Abstract :

Recently, the application of drugs administered for reasons other than male infertility may have profound effect on fertility potential. Ibuprofen, as one of the non-steroidal anti-inflammatory drugs can possibly have a negative effect on the spermatogenesis process which could be dose-dependent. This side effect is likely acquired through oxidative stress. Therefore, in this study, we assessed the effect of different doses of Ibuprofen on spermatogenesis, sperm parameters, and oxidative stress. In this study, 15 male NMRI mice were daily gavaged by different doses of Ibuprofen (0, 5, 25, 50, and 100 mg/kg) for one month. Then, morphometric parameters of testis, sperm concentration, motility and oxidative stress were assessed. Finally, the data were analyzed using SPSS software and ANOVA test. Different doses of Ibuprofen had no effect on Johnson score and sperm concentration as compared to the control group. However, sperm motility was insignificantly reduced only at a dose of 100 mg / kg. In addition, the level of oxidative stress in all doses of Ibuprofen increased compared to the control group and, this increase was only significant at the dose of 100 mg/kg (P=0.03). Based on the results of this study, continuous administration of Ibuprofen for one month has no significant effect on spermatogenesis and sperm parameters but significantly increase ROS production, the effect of which on sperm chromatin integrity, for longer period remain a concern, which needs more studies in the future.  

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