Effects of different concentrations of lead on some biochemical and physiological parameters of rice (Oryza sativa L.)
Subject Areas : GeneticJavad Salvati 1 , Hormoz Fallah 2 , yousof niknejad 3 , Davood Barari Tari 4
1 - Department of Agriculture, Ayatollah Amoly Branch, Islamic Azad University, Amol, Iran
2 - Department of Agronomy, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
3 - Department of Agronomy, Ayatollah Amoli Branch, Islamic Azad University, Amol, IRAN
4 - Asistant professor of Agrotechnology Department, Amol Branch, Islamic Azad University, Amol, Iran.
Keywords: Lead stress, Rice, Proline metabolism, Oxidative stress, Chlorophyll metabolism,
Abstract :
Lead (Pb) not only negatively alters plant growth and yield but may also have potentially toxic risks to human health. In the present study, the effects of different concentrations of Pb (150 and 300 μM) on growth, physiological and biochemical attributes of rice under hydroponic conditions were investigated. The results showed that Pb treatments with negative effects on chlorophyll metabolism reduced photosynthetic pigments and, consequently, diminished the growth and biomass of rice plants. Pb stress induced oxidative stress and damage to bio-membranes by increasing the accumulation of hydrogen peroxide and methylglyoxal. The activity of antioxidant enzymes and glyoxalase cycle was upregulated in rice leaves under Pb toxicity. Pb treatments altered the levels of non-enzymatic antioxidant compounds (glutathione (GSH) and ascorbic acid (ASA)) in the leaves of rice plants by reducing the ratios of reduced ASA to oxidized ASA and reduced GSH to oxidized GSH compared to control plants. Therefore, concentrations of 150 and 300 μM Pb in the soil can induce negative effects on important physiological and metabolic processes of rice, which reduce plant growth and biomass.
Ahmad, P., Alam, P., Balawi, T.H., Altalayan, F.H., Ahanger, M.A. and Ashraf, M. (2020). Sodium nitroprusside (SNP) improves tolerance to arsenic (As) toxicity in Vicia faba through the modifications of biochemical attributes, antioxidants, ascorbate-glutathione cycle and glyoxalase cycle. Chemosphere. 244: 125480
Alexieva, V., Sergiev, I., Mapelli, S. and Karanov, E. (2001). The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. Plant, Cell & Environment. 24: 1337-44.
Ali, B., Xu, X., Gill, R.A., Yang, S., Ali, S., Tahir, M. and Zhou, W. (2014). Promotive role of 5-aminolevulinic acid on mineral nutrients and antioxidative defense system under lead toxicity in Brassica napus. Industrial Crops and Products. 52: 617–626.
Anjum, S.A., Ashraf, U., Khan, I., Tanveer, M., Ali, M., Hussain, I. and Wang L.C. (2016). Chromium and aluminum phytotoxicity in maize: morphophysiological responses and metal uptake. Clean Soil Air Water. 44: 1–10.
Ashraf, U., Kanu, A.S., Deng, Q., Mo, Z., Pan, S., Tian, H. and Tang, X. (2017). Lead (Pb) toxicity; physio-biochemical mechanisms, grain yield, quality, and pb distribution proportions in scented rice. Frontiers in Plant Science. 8: 259.
Ashraf, U., Kanu, A.S., Mo, Z.W., Hussain, S., Anjum, S.A., Khan, I., Abbas, R.N. and Tang, X. (2015). Lead toxicity in rice; effects, mechanisms and mitigation strategies-a mini review. Environmental Science and Pollution Research. 22: 18318–18332.
Bali, S., Kaur, P., Kohli, S.K., Ohri, P., Thukral, A.K., Bhardwaj, R., Wijaya, L., Alyemeni, M.N. and Ahmad, P. (2018). Jasmonic acid induced changes in physio-biochemical attributes and ascorbate-glutathione pathway in Lycopersicon esculentum under lead stress at different growth stages. Science of the Total Environment. 645: 1344–1360
Bates, L.S., Waldern, R.P. and Tear, I.D. (1973). Rapid determination of free proline for water stress studies. Plant and Soil. 39: 205-207.
Beauchamp, C. and Fridovich, I. (1971). Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry. 44: 276-287.
Charest, C. and Phan, C.T. (1990). Cold-acclimation of wheat (Triticum Aestivum) - properties of enzymes involved in proline metabolism. Physiologia Plantarum. 80: 159–168.
Clemens, S. (2006). Toxic metal accumulation, response to exposure and mechanism of tolerance in plants. Biochimie. 88: 1707–1719.
Costa, M.L., Civello, P.M., Chaves, A.R. and Martínez, G.A. (2005). Effect of ethephon and 6–benzylaminopurine on chlorophyll degrading enzymes and a peroxidase–linked chlorophyll bleaching during post–harvest senescence of broccoli (Brassica oleracea L.) at 20 C. Postharvest Biology and Technology. 35(2): 191–9.
Dutilleul, C., Driscoll, S., Cornic, G., De Paepe, R., Foyer, C.H. and Noctor, G. (2003). Functional mitochondrial complex I is required by tobacco leaves for optimal photosynthetic performance in photorespiratory conditions and during transients. Plant Physiology. 131: 264–275.
Garg, N. and Manchanda, G. (2009). ROS generation in plants: boon or bane? Plant Biosystems. 143: 8–96.
Gerami, M., Ghorbani, A. and Karimi, S. (2018). Role of salicylic acid pretreatment in alleviating cadmium-induced toxicity in Salvia officinalis L. Iranian Journal of Plant Biology. 10(1): 81–95.
Ghasemi-Omran, V.O., Ghorbani, A. and Sajjadi-Otaghsara, S.A. (2021). Melatonin alleviates NaCl-induced damage by regulating ionic homeostasis, antioxidant system, redox homeostasis, and expression of steviol glycosides-related biosynthetic genes in in vitro cultured Stevia rebaudiana Bertoni. In Vitro Cellular and Developmental Biology. 57: 319–331.
Ghorbani, A., Ghasemi Omran, V.O., Razavi, S.M., Pirdashti, H. and Ranjbar, M. (2019a). Piriformospora indica confers salinity tolerance on tomato (Lycopersicon esculentum Mill.) through amelioration of nutrient accumulation, K+/Na+ homeostasis and water status. Plant Cell Reports. 38: 1151–1163.
Ghorbani, A., Pishkar, L., Roodbari, N., Pehlivan, N. and Wu, C. (2021). Nitric oxide could allay arsenic phytotoxicity in tomato (Solanum lycopersicum L.) by modulating photosynthetic pigments, phytochelatin metabolism, molecular redox status and arsenic sequestration. Plant Physiology and Biochemistry. 167: 337–348.
Ghorbani, A., Razavi, S.M., Ghasemi Omran, V. and Pirdeshti, H. (2019b). Effects of endophyte fungi symbiosis on some physiological parameters of tomato plants under 10 day long salinity stress. Journal of Plant Process and Function. 7(27): 193–208.
Ghorbani, A., Razavi, S.M., Ghasemi Omran, V.O. and Pirdashti, H. (2018a). Piriformospora indica alleviates salinity by boosting redox poise and antioxidative potential of tomato. Russian Journal of Plant Physiology. 65: 898–907.
Ghorbani, A., Razavi, S.M., Ghasemi Omran, V.O. and Pirdashti, H. (2018b). Piriformospora indica inoculation alleviates the adverse effect of NaCl stress on growth, gas exchange and chlorophyll fluorescence in tomato (Solanum lycopersicum L.). Plant Biology 20: 729–736.
Ghorbani, A., Tafteh, M., Roudbari, N., Pishkar, L., Zhang, W. and Wu, C. (2020). Piriformospora indica augments arsenic tolerance in rice (Oryza sativa) by immobilizing arsenic in roots and improving iron translocation to shoots. Ecotoxicology and Environmental Safety. 209: 111793.
Ghorbani, A., Zarinkamar, F. and Fallah, A. (2009). The effect of cold stress on the morphologic and physiologic characters of tow rice varieties in seedling stage. Journal of Crop Breeding. 1: 50–66.
Ghorbani, A., Zarinkamar, F. and Fallah, A. (2011). Effect of cold stress on the anatomy and morphology of the tolerant and sensitive cultivars of rice during germination. Journal of Cell & Tissue. 2(3): 235–244.
Gill, M. (2014). Heavy metal stress in plants: a review. International Journal of Advanced Research. 2(6): 1043-1055.
Hall, J.L. (2002) Cellular mechanisms for heavy metal detoxification and tolerance. Journal of Experimental Botany. 53: 1–11
Hasan, M.K., Ahammed, G.J., Yin, L., Shi, K., Xia, X., Zhou, Y., Yu, J. and Zhou, J. (2015). Melatonin mitigates cadmium phytotoxicity through modulation of phytochelatins biosynthesis, vacuolar sequestration, and antioxidant potential in Solanum lycopersicum L. Frontiers in Plant Science. 6: 601
Hasanuzzaman, M., Hossain, M.A. and Fujita, M. (2011). Nitric oxide modulates antioxidant defense and methylglyoxal detoxification system and reduces salinity induced damage in wheat seedling. Plant Biotechnology Reports. 5: 353–365.
Heath, R.L. and Packer, L. (1968). Photoperoxidation in isolated chloroplasts. Archives of Biochemistry and Biophysics. 125: 189–198.
Hegedus, A., Erdel, S. and Horvath, G. (2001). Comparative studies of H2O2 detoxifying enzymes in green and greening barley seedlings under Cd stress. Plant Science. 160: 1085–1093.
Jain, M. and Gadre, R. (2004). Inhibition of chlorophyll biosynthesis by mercury in excised etiolated maize leaf segments during greening: effect of 2-oxoglutarate. Indian Journal of Experimental Biology. 42: 419–423.
Kumar, A., Prasad, M.N.V. and Sytar, O. (2012). Lead toxicity, defense strategies and associated indicative biomarkers in Talinum triangulare grown hydroponically. Chemosphere. 89: 1056–1065.
Kupper, H., Kupper, F. and Spiller, M. (1996). Environmental relevance of heavy metal substituted chlorophylls using the example of water plants. Journal of Experimental Botany. 47: 259–266.
Lamhamdi, M., Bakrim, A., Aarab, A., Lafont, R. and Sayah, F. (2011). Effects of lead phytotoxicity on wheat (Triticum aestivum L.) seed germination and seedling growth. Comptes Rendus Biologies. 334: 118–126.
Lichtenthaler, H.K. (1987). Chlorophyll and carotenoids: Pigments of photosynthetic biomembrane. Methods in Enzymology. 148: 350-381.
Mittler, R. (2002). Oxidative stress, antioxidant and stress tolerance. Trends in Plant Science. 7: 841–851.
Mittler, R., Finka, A. and Goloubinoff, P. (2012). How do plants feel the heat? Trends in Biochemical Sciences. 37: 118–125.
Mostofa, M.G., Hossain, M.A., Fujita, M. and Tran, L.S. (2015). Physiological and biochemical mechanisms associated with trehalose-induced copper-stress tolerance in rice. Scientific Reports. 5: 11433.
Namdjoyan, S., Soorki, A.A., Elyasi, N., Kazemi, N. and Simaei, M. (2020). Melatonin alleviates lead-induced oxidative damage in safflower (Carthamus tinctorius L.) seedlings. Ecotoxicology. 29(1): 108–118.
Piechalak, A., Tomaszewska, B., Baralkiewicz, D. and Malecka, A. (2002). Accumulation and detoxification of lead ions in legumes. Phytochemistry. 60: 153–162.
Piotrowska, A., Bajguz, A., Godlewska-Zyłkiewicz, B., Czerpak, R. and Kamińska, M. (2009). Jasmonic acid as modulator of lead toxicity in aquatic plant Wolffia arrhiza (Lemnaceae). Environmental and Experimental Botany. 66: 507–513.
Pourrut, B., Shahid, M., Dumat, C., Winterton, P. and Pinelli, E. (2011). Lead uptake, toxicity, and detoxification in plants. Reviews of Environmental Contamination and Toxicology. 213: 113–136.
Principato, G.B., Rosi, G., Talesa, V., Govannini, E. and Uotila, L. (1987). Purification and characterization of two forms of glyoxalase II from rat liver and brain of Wistar rats. Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 911: 349–355.
Sairam, R.K., Rao, K.V. and Srivastava, G.C. (2002). Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Science. 163: 1037-1046.
Shakoor, M.B., Ali, S., Hameed, A., Farid, M., Hussain, S., Yasmeen, T., Najeeb, U., Bharwana, S.A. and Abbasi, G.H. (2014). Citric acid improves lead (pb) phytoextraction in Brassica napus L. by mitigating pb-induced morphological and biochemical damages. Ecotoxicology and Environmental Safety. 109: 38–47.
Sharma, P. and Dubey, R.S. (2005). Lead toxicity in plants. Brazilian Journal of Plant Physiology. 17: 35–52.
Singh, R., Tripathi, R.D., Dwivedi, S., Kumar, A., Trivedi, P.K. and Chakrabarty D. (2010). Lead bioaccumulation potential of an aquatic macrophyte Najas indica are related to antioxidant system. Bioresource Technology. 101: 3025–3032.
Srivastava, R.K., Pandey, P., Rajpoot, R., Rani, A. and Dubey, R.S. (2014). Cadmium and lead interactive effects on oxidative stress and antioxidative responses in rice seedlings. Protoplasma. 251: 1047–1065.
Sumithra, K., Jutur, P.P., Carmel, B.D. and Reddy, A.R. (2006). Salinity-induced changes in two cultivars of Vigna radiata: responses of antioxidative and proline metabolism. Plant Growth Regulation. 50: 11–22.
Wild, R., Ooi, L., Srikanth, V. and Münch, G. (2012). A quick, convenient and economical method for the reliable determination of methylglyoxal in millimolar concentrations: the N-acetyl-L-cysteine assay. Analytical and Bioanalytical Chemistry. 403: 2577–2581.
