THE EFFECT OF D-GALACTOSE INDUCED MICE AGING ON CEREBELLAR TISSUE
Subject Areas : Histology
1 - Division of Pharmacology and Toxicology, Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Keywords: Aging, D- galactose, Mouse, Oxidative stress, Cerebellum,
Abstract :
Cerebellum is an important area of hind brain that plays key role in balancing, controlling excitement & subtle acts. Studies have shown that prolonged use of de-galactose in mice leads to morphological & functional disorders of brain like normal aging symptoms. The study aimed to evaluate the possible changes of cerebellar cortex tissue, ROS test of active oxygen species of cerebellum tissue following aging induction by D-galactose in house mouse (Mus musculus). According to study aim, age-old modeling was performed on all house mouse. Accordingly, subjects were randomly assigned in two groups: control + saline (S + C) & aging group (de-galactose). De-galactose & saline solution were used in order to create an aging model (sodium chloride 0.9%). The cerebellum samples were out by sectional thickness of 5 microns after preparation & tissue passage stages & were stained by hematoxylin-eosin & finally were examined under optical Nikon microscope. Quantitative variables were analyzed using T-test & SPSS software. The most variations were observed in Purkinje cells in observations on cerebellum tissue samples of aged-induced group by D-galactose. The arrangement & placement of cells were destroyed. The position of nuclei in cell was not central. Purkinje cells had different morphological forms under aging induction. Necrosis, chromatolysis & picnosis were observed. According to results, di-galactose (aging induction) causes pathological changes in cerebellar cortex, especially in porkinje.
