Effect of P-cymene on Chronic Immobilization-induced Oxidative Stress in Testis of Adult Male Rats
Subject Areas : Journal of Animal Biology
Yasaman Peirovy
1
(Nanobiotechnology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran)
masoumeh aslerousta
2
(Department of Physiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran)
Keywords: P-cymene, Testis, Oxidative stress, Chronic immobilization, Rat,
Abstract :
Chronic immobility is considered a stress that damages various body organs by inducing oxidative stress. In the study, the effect of the monoterpene p-cymene on oxidative stress induced by chronic immobility was investigated in the testes of adult male Wistar rats. Forty rats were divided into four groups (10 rats each) control, cymene, stress, and stress-cymene. The stress and stress-cymene groups were placed in the restrainer for 2.5 hours every day for 14 consecutive days. Cymene and stress-cymene groups also received p-Simon (50 mg/kg) intraperitoneally during the same period. At the end of the course, the levels of malondialdehyde, glutathione, the activity of superoxide dismutase, and glutathione peroxidase enzymes were measured in the testicles of the animals. The level of malondialdehyde in the stress-cymene group was significantly lower (P < 0.01), while the level of glutathione was higher in this group compared to the stress group (P < 0.05). The activity of superoxide dismutase and glutathione peroxidase showed a significant increase in the stress-cymene group compared to the stress group (P < 0.001). It is concluded that p-cymene can reduce the oxidative stress caused by chronic immobility in the testes of rats and possibly has therapeutic potential for testicular dysfunction.
1. Abouzaripour M., Daneshi E., Amiri F., Naseri S. 2023. Investigating the effect of Nigella sativa on the testicular function of first-generation offspring of mice treated with titanium oxide nanoparticles. Avicenna Journal of Phytomedicine, 13(5):541-550.
2. Al-Damegh M.A. 2014. Stress-induced changes in testosterone secretion in male rats: role of oxidative stress and modulation by antioxidants. Open Journal of Animal Sciences, 4(02):70-78.
3. Arabloei Sani M., Yaghmaei P., Hajebrahimi Z., Hayati Roodbari N. 2022. Therapeutic effect of P-cymene on lipid profile, liver enzyme, and Akt/Mtor pathway in streptozotocin-induced diabetes mellitus in wistar rats. Journal of Obesity, 2022:1015669-1015685.
4. Badade Z.G., More K., Narshetty J. 2011. Oxidative stress adversely affects spermatogenesis in male infertility. Biomedical Research, 22(3): 319-322.
5. Balahbib A., El Omari N., Hachlafi N.E., Lakhdar F., El Menyiy N., Salhi N., Mrabti H.N., Bakrim S., Zengin G., Bouyahya A. 2021. Health beneficial and pharmacological properties of p-cymene. Food and Chemical Toxicology, 153: 112259-112271.
6. Chen L., Zhao L., Zhang C., Lan Z. 2014. Protective effect of p-cymene on lipopolysaccharide-induced acute lung injury in mice. Inflammation, 37:358-364.
7. de Oliveira T.M., de Carvalho R.B., da Costa I.H., de Oliveira G.A., de Souza A.A., de Lima S.G., de Freitas R.M.. 2015. Evaluation of p-cymene, a natural antioxidant. Pharmaceutical Biology, 53(3):423-428.
8. de Santana M.F., Guimarães A.G., Chaves D.O., Silva J.C., Bonjardim L.R., Lucca Júnior W.D., Ferro J.N., Barreto E.D., Santos F.E., Soares M.B., Villarreal C.F. 2015. The anti-hyperalgesic and anti-inflammatory profiles of p-cymene: Evidence for the involvement of opioid system and cytokines. Pharmaceutical Biology, 53(11):1583-1590.
9. Dutta S., Sengupta P., Slama P., Roychoudhury S. 2021. Oxidative stress, testicular inflammatory pathways, and male reproduction. International Journal of Molecular Sciences, 22(18):10043.
10. Hall J.E., Hall M.E. 2006. Guyton and Hall textbook of medical physiology e-Book. 11th ed. Elsevier Health Sciences, Philadelphia.
11. Ighodaro O.M., Akinloye O.A. 2018. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria Journal of Medicine, 54(4):287-293.
12. Jaggi A.S., Bhatia N., Kumar N., Singh N., Anand P., Dhawan R. 2011. A review on animal models for screening potential anti-stress agents. Neurological Sciences, 32:993-1005.
13. Joo J.S., Park K., Ahn K.Y., Park Y.I. 2006. The Effect of Long-term Immobilization Stress on Spermatogenesis and Testosterone Production. Korean Journal of Urology, 47(11): 1197-1203.
14. Karna K.K., Soni K.K., You J.H., Choi N.Y., Kim H.K., Kim C.Y., Lee S.W., Shin Y.S., Park J.K. 2020. MOTILIPERM ameliorates immobilization stress-induced testicular dysfunction via inhibition of oxidative stress and modulation of the Nrf2/HO-1 pathway in SD rats. International Journal of Molecular Sciences, 21(13):4750.
15. Kazemi S., Safari S., Komaki S., Karimi S.A., Golipoor Z., Komaki A. 2023. The effects of carvacrol and p‐cymene on Aβ1‐42‐induced long‐term potentiation deficit in male rats. CNS Neuroscience and Therapeutics, 30(3):e14459.
16. Kurkowska W., Bogacz A., Janiszewska M., Gabryś E., Tiszler M., Bellanti F., Kasperczyk S., Machoń-Grecka A., Dobrakowski M., Kasperczyk A. 2020. Oxidative stress is associated with reduced sperm motility in normal semen. American Journal of Men's Health, 14(5): 1557988320939731.
17. Li X., Zhang B., Yang H., Zhang L., Lou H., Zheng S. 2024. The emergence of natural products as potential therapeutics for male infertility. Andrology, 2024:1-18.
18. Lowry O., Rosebrough N., Farr A., Randall R. 1951. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193(1): 265-275.
19. Mehfooz A., Wei Q., Zheng K., Fadlalla M.B., Maltasic G., Shi F. 2018. Protective roles of Rutin against restraint stress on spermatogenesis in testes of adult mice. Tissue and Cell, 50:133-143.
20. Noh S., Go A., Kim D.B., Park M., Jeon H.W., Kim B. 2020. Role of antioxidant natural products in management of infertility: a review of their medicinal potential. Antioxidants, 9(10): 957.
21. Olave F.A., Aguayo F.I., Román-Albasini L., Corrales W.A., Silva J.P., González P.I., Lagos S., García M.A., Alarcón-Mardones M., Rojas P.S., Xu X. 2022. Chronic restraint stress produces sex-specific behavioral and molecular outcomes in the dorsal and ventral rat hippocampus. Neurobiology of Stress, 17: 100440.
22. Shareef S.H., Al-Medhtiy M.H., Ibrahim I.A., Alzahrani A.R., Jabbar A.A., Galali Y., Agha N.F., Aziz P.Y., Thabit M.A., Agha D.N., Salehen N.A. 2022. Gastroprophylactic effects of p-cymene in ethanol-induced gastric ulcer in rats. Processes, 10(7):1314-1333.
23. Tsigos C., Kyrou I., Kassi E. 2000. Stress: Endocrine Physiology and Pathophysiology. Endotext [Internet].
24. Turner T.T., Lysiak J.J. 2008. Oxidative stress: a common factor in testicular dysfunction. Journal of Andrology, 29(5):488-498.
25. Yadav A., Yadav K., Rajpoot A., Lal B., Mishra R.K. 2022. Sub‐chronic restraint stress exposure in adult rats: An insight into possible inhibitory mechanism on testicular function in relation to germ cell dynamics. Andrologia, 54(11):e14575-e14591.
