Antioxidant enzyme activities and fermentation metabolism in the root of three wheat promising lines under waterlogging stress

Subject Areas : Journal of Plant Ecophysiology

Freshteh alizade vaskasi ¹ , hemmatollah Pirdashti ² , Ali Cherati_Araei ³ , Sara Saadatmand ⁴

1 - Department of Biology, Basic Science Campus, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Agronomy, Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari agricultural science and natural resources university, Sari, Iran
3 - Agricultural and Natural Resources Research Center of Mazandaran, Iran
4 - Department of Biology, Basic Science Campus, Science and Research Branch, Islamic Azad University, Tehran, Iran

Received: 2018-12-24 Accepted : 2019-05-11 Published : 2020-09-22

Keywords: Proline, Oxidative stress, Lactate dehydrogenase, Antioxidant enzymes, Alcohol dehydrogenase,

Abstract :

Waterlogging stress has negative effects on the growth and yield of wheat plants, which recognizes the defense mechanism of the plant against waterlogging, can be valuable. In order to study the response of three wheat genotypes to different levels of waterlogging stress, a factorial experiment was conducted based on a completely randomized design with five replications in Gharakhil (Ghaemshahr) Agricultural Station. The objective of this study was to investigate the effects of waterlogging (0, 7, 14 and 21 day) at tillering (ZG21) and stem elongation (ZG31) stages on the growth, total chlorophyll, proline, malondialdehyde (MDA), H2O2, activity of antioxidant enzymes, and enzymes of fermentation pathway in root of three wheat promising lines (N-93-19, N-93-9 and N-92-9). The results showed that waterlogging stress in both growth stages reduced the total chlorophyll and growth of all three genotypes, however, the highest contents of total chlorophyll and shoot and root dry weight were observed in N-92-9 genotype. Increased waterlogging stress increased the contents of proline, MDA, H2O2, activity of superoxide dismutase, catalase, alcohol dehydrogenase and lactate dehydrogenase and decreased peroxidase activity of root of genotypes in both growth stages compared to control treatments. The results of this experiment that N-92-9 genotype had better response than other two genotypes in all studied traits under waterlogging conditions and was introduced as a flood tolerant genotype.

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