The effect of Holothuria atra body wall extract on pulmonary fibrosis and oxidative stress caused by bleomycin in Wistar rat
Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)
Hassan Mehdizadeh kakaei
1
,
Ali Hosseini Sharifabad
2
,
Afsaneh Yegdaneh
3
,
Adel Mohammad Alipour
4
1 -
2 - , department of pharmacology and toxicology, Isfahan University of Medical Sciences, Isfahan, Iran.
3 -
4 -
Keywords: Bleomycin sulfate, Pulmonary fibrosis, Oxidative stress, Holothuria atra,
Abstract :
Background & Aim: Idiopathic pulmonary fibrosis (IPF) leads to the misplaced deposition of the extracellular matrix and the stiffness parenchyma and the creation of scarring (fibrosis) in the lung. This study was conducted with the aim of investigating the effectiveness of different doses of H.atra body wall extract on bleomycin-induced pulmonary fibrosis. Experimental: The animals were randomly divided into six groups (n = 6). Group 1 (Control) was administered normal saline intratracheally (single dose) on day 1, Group 2 (bleomycin) received single dose of bleomycin (BLM, 7.5 IU/kg) intratracheally and daily doses of normal saline by gavage, Group 3 (pirfenidone) received single dose of BLM (7.5 IU/kg) intratracheally and daily doses of pirfenidone by gavage, Groups (4, 5 and 6) received single dose of BLM (7.5 IU/kg) intratracheally and received daily doses of H. atra extract (50, 100 and 200 mg/kg, respectively). Finally, the rats were euthanized, and the lung tissues were taken out for histological and biochemical assessments. Results: Histological tests showed that bleomycin could induce marked pulmonary fibrosis within two weeks. Administration of hydroalcoholic extract of H. atra body wall reduced these damages in lung tissue in a dose-dependent manner. The best results were obtained for 200 mg/kg per day of H. atra body wall extract. Recommended applications/industries: Based on the present findings, H. atra body wall extract can reduce the toxic effects of bleomycin on lung tissues. Such effects can be attributed to the compounds with anti-inflammatory and antioxidant properties in this plant.
Awad, S.N., Abd El Hafez, M.S., AbdElwahab, M.G., Mady, R., Batiha, G.E.S., Shaheen, H.M. and Ghareeb, D.A. 2023. Anti-inflammatory properties of Holothuria atra extract on lipopolysaccharide induced inflammation of White blood cells. Damanhour Journal of Veterinary Sciences, 9(2) :9-16.
Chandra, R. and Alam, A. 2022. Phytochemical analysis using X-ray diffraction spectroscopy (XRD) and GC-MS analysis of bioactive compounds in Cucumis sativus L. (Angiosperms; Cucurbitaceae). The Scientific Temper, 13(01): 124-131.
Del Rio, D., Stewart, A.J. and Pellegrini, N. 2005.A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutrition, Metabolism and Cardiovascular Diseases, 15(4): 316-328.
Desai, R., Katukuri, N., Kanagala, S.G., Ghadge, N., James, A., Tajdin, B., Nalla, A., Vutukuru, S.D., Kotharu, D.M., Kadiyala, A. and Vyas, A. 2024. Examining prevalence and predictors of pulmonary hypertension in adults with idiopathic pulmonary fibrosis: a population-based analysis in the United States. Journal of Medicine and Life, 17(1):35-39.
Dhaouafi, J., Abidi, A., Nedjar, N., Romdhani, M., Tounsi, H., Sebai, H. and Balti, R. 2023. Protective effect of Tunisian red seaweed (Corallina officinalis) against bleomycin-induced pulmonary fibrosis and oxidative stress in rats. Dose-Response, 21(2):15593258231179906.
Esfahani, M., Rahbar, A.H., Asl, S.S., Bashirian, S., Moeini, E.S.M. and Mehri, F. 2023. The effects of resveratrol on silica-induced lung oxidative stress and inflammation in rat. Safety and Health at Work, 14(1):118-123.
Esmat, A.Y., Said, M.M., Soliman, A.A., El-Masry, K.S. and Badiea, E.A. 2013. Bioactive compounds, antioxidant potential, and hepatoprotective activity of sea cucumber (Holothuria atra) against thioacetamide intoxication in rats. Nutrition, 29(1): 258-267.
Fernandez, I.E. and Eickelberg, O. 2012. New cellular and molecular mechanisms of lung injury and fibrosis in idiopathic pulmonary fibrosis. The Lancet, 380(9842): 680-688.
Ghelani, H., Khursheed, M., Adrian, T.E. and Jan, R.K. 2022. Anti-inflammatory effects of compounds from echinoderms. Marine Drugs, 20(11): 693-700.
Glass, D.S., Grossfeld, D., Renna, H.A., Agarwala, P., Spiegler, P., DeLeon, J. and Reiss, A.B. 2022. Idiopathic pulmonary fibrosis: Current and future treatment. The Clinical Respiratory Journal, 16(2): 84-96.
Hemmati, A.A., Jalali, A. and Keshavarz, P., 2018.Effect of chamomile hydroalcoholic extract on Bleomycin-induced pulmonary fibrosis in rat. Tanaffos, 17(4): 264.
Hosseini-Sharifabad, A., Sadraei, H., Hashemnia, M., Sajjadi, S.E. and Mirdamadi, Z. 2021. Effect of hydroalcoholic and aqueous extracts of Dracocephalumkotschyi on bleomycin induced pulmonary fibrosis. Journal of Herbmed Pharmacology, 10(2): 209-217.
Khotimchenko, Y. 2018. Pharmacological potential of sea cucumbers. International Journal of Molecular Sciences, 19(5): 1342.
Kim, H.J., Perlman, D. and Tomic, R. 2015.Natural history of idiopathic pulmonary fibrosis. Respiratory Medicine, 109(6):661-670.
Mahlooji, M.A., Heshmati, A., Kheiripour, N., Ghasemi, H., Asl, S.S., Solgi, G., Ranjbar, A. and Hosseini, A. 2022. Evaluation of protective effects of curcumin and nanocurcumin on aluminium phosphide induced subacute lung injury in rats: modulation of oxidative stress through SIRT1/FOXO3 signalling pathway. Drug Research, 72(02):100-108.
Meyer, K.C. and Decker, C.A. 2017. Role of pirfenidone in the management of pulmonary fibrosis. Therapeutics and Clinical Risk Management, 13: 427-437.
Nishikawa, Y., Furukawa, A., Shiga, I., Muroi, Y., Ishii, T., Hongo, Y., Takahashi, S., Sugawara, T., Koshino, H. and Ohnishi, M. 2015. Cytoprotective effects of lysophospholipids from sea cucumber Holothuria atra. PLoS One, 10(8):e0135701.
Otoupalova, E., Smith, S., Cheng, G. and Thannickal, V.J. 2011. Oxidative stress in pulmonary fibrosis. Comprehensive Physiology, 10(2): 509-547.
Peikert, T., Daniels, C.E., Beebe, T.J., Meyer, K.C. and Ryu, J.H. 2008. Assessment of current practice in the diagnosis and therapy of idiopathic pulmonary fibrosis. Respiratory Medicine, 102(9): 1342-1348.
Puspitasari, Y.E., Tuenter, E., Foubert, K., Herawati, H., Hariati, A.M., Aulanni’am, A.A., Pieters, L., De Bruyne, T. and Hermans, N. 2023.Saponin and fatty acid profiling of the sea cucumber Holothuria atra, α-glucosidase inhibitory activity and the identification of a novel triterpene glycoside. Nutrients, 15(4):1033.
Safaeian, L., Jafarian, A., Rabani, M., Mirmohammad, S.H., Torabinia, N. and ALAVI, S. 2009. The effect of AT1 receptor blockade on bax and bcl-2 expression in bleomycin-induced pulmonary fibrosis. Daru. 17 (1): 53-58.
Savin, I.A., Zenkova, M.A. and Sen’kova, A.V. 2022. Pulmonary fibrosis as a result of acute lung inflammation: molecular mechanisms, relevant in vivo models, prognostic and therapeutic approaches. International Journal of Molecular Sciences, 23(23): 14959.
Tolle, L.B., Southern, B.D., Culver, D.A. and Horowitz, J.C. 2018. Idiopathic pulmonary fibrosis: What primary care physicians need to know. Cleveland Clinic Journal Medicine, 85(5): 377-386.
Woo, S.P. 2018. Systematic Studies on Sea Cucumbers of the Family Stichopodidae (Echinodermata: Holothuroidea). Doctoral dissertation. Hokkaido University.
Yang, J., Cui, Y. and Kolb, M. 2009. How useful is traditional herbal medicine for pulmonary fibrosis?. Respirology, 14(8): 1082-1091.
Zaafan, M.A., Zaki, H.F., El-Brairy, A.I. and Kenawy, S.A. 2016. Pyrrolidinedithiocarbamate attenuates bleomycin-induced pulmonary fibrosis in rats: modulation of oxidative stress, fibrosis, and inflammatory parameters. Experimental Lung Research, 42(8-10): 408-416.
