List of Articles Ebrahim Rahimi

• Open Access Article
  • Abstract Page
  • Full-Text
  
  1 - Isolation of Alicyclobacillus from fruits juice in Iran
  Ebrahim Rahimi Abbas Doosti Sara Dehghan Nejad
  Heat-loving bacteria Bacillus Alicyclobacilluschanging flavor juices and ready to use in recent years in a wide range of large amounts of fruit juice was corrupt world, although there is no previous report indicating its pathogenesis. This research was done to study con More

  Heat-loving bacteria Bacillus Alicyclobacilluschanging flavor juices and ready to use in recent years in a wide range of large amounts of fruit juice was corrupt world, although there is no previous report indicating its pathogenesis. This research was done to study contamination of packaged juice with the bacteria. A total of 300 samples of pomegranate juice, apple juice, orange juice, mangoes juice, pineapple juice, grape juice (from different companies) at Esfahan was collected and studied for Alicyclobacillus contamination. The samples were tested using culture, biochemical tests and PCR. According to the results, 13 samples (4.33%) were contaminated with Alicyclobacillus. The highest level of contamination among samples was found in pomegranate juice and orange juice (13.3%). Contamination in cranberry juice, mixed species and other samples was 10, and 6.66 %. No contamination was found the other species. Regarding to the obtained results, prevention of bacterial growth especially during storage time and detection of bacteria after pasteurization is necessary. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  2 - A review of Pseudomonas aeruginosa in the environment: pathogenesis, antibiotic resistance, genetic diversity, and removal ways from water treatment
  Ghasem Ghorbani Ebrahim Rahimi Amir Shakerian
  10.30495/jfm.2023.1974180.1798
  Pseudomonas aeruginosa as a waterborne pathogen is a growing concern to public health sectors. Many sources of environmental water could potentially be acting as a reservoir for potentially pathogenic strains of P. aeruginosa. Due to the fact that P. aeruginosa is an op More

  Pseudomonas aeruginosa as a waterborne pathogen is a growing concern to public health sectors. Many sources of environmental water could potentially be acting as a reservoir for potentially pathogenic strains of P. aeruginosa. Due to the fact that P. aeruginosa is an opportunistic pathogen and causes serious problems, it seems necessary to know the pathogenesis, antibiotic resistance, genetic diversity and methods of removing it from drinking water. The pathogenesis of P. aeruginosa has been extensively studied and proven to be a multifactorial process, mediated by quorum sensing. P. aeruginosa possess two quorum sensing systems, las and rhl that facilitate cell to cell communication through production of signalling molecules termed autoinducers to target specific receptors for activation. P. aeruginosa is intrinsically resistant to various antibiotics due to a low permeability in the outer membrane, which acts as a selective barrier. However, this bacterium is a highly diverse pathogen that is capable of adaptation to the surrounding environment. When subjected to antibiotic selective pressure, the induced response facilitates bacterial survival and develops antibiotic resistance. literature suggests that the large genome size and genome complexity are responsible for the ability of this bacterium to adapt and thrive in a diverse range of environments. The presence and persistence of these bacteria in environmental waters may pose a great risk to the public health and requires further work to fully characterize and quantify the input of MDR P. aeruginosa strains from the hospitals compared with those originating from the general community or other wastewater related sources. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  3 - Prevalence and antimicrobial resistance of Listeria species isolated from filleted Argyrosomus hololepidotus, Scomberomorus commerson and Alburnus spp.
  Ebrahim Rahimi Mohammad Javad Jahanmard Sohrab safari Mahsa Ansari Zeinab Torki baghbadorani
  Listeriosis is one of the most important food-borne diseases caused by Listeria species, especially L. monocytogenes.  The objective of this study was to determine the prevalence and antimicrobial resistance of Listeria species isolated from three types of fish fil More

  Listeriosis is one of the most important food-borne diseases caused by Listeria species, especially L. monocytogenes.  The objective of this study was to determine the prevalence and antimicrobial resistance of Listeria species isolated from three types of fish filet in Isfahan and Bandaranzali. From August 2009 to April 2011, a total of 120 samples of Argyrosomus hololepidotus (n= 90), Scomberomorus commerson (n=80) and Alburnus spp (n=70). Fish were obtained from randomly selected retail stores in Isfahan and Bandaranzali cities and were evaluated for the presence of Listeria spp. using standard cultural and PCR methods. Then antibiogram tests were carried out for determination of antimicrobial resistance. Seven (8.8%) and 6 (15%) of smoked and salted fish samples were positive for Listeria spp. respectively. L. monocytogenes, L. innocua and L. seeligari were isolated from 2.5, 6.7 and 1.6% of fish samples. Overall, 9 of 13 Listeria isolates (69.2%) were resistant to one or more antimicrobial agents. Resistance to tetracycline (53.8%) and tetracycline (30.8%) were the most common finding. All of the isolates were susceptible to gentamicin, vancomycin and chloramphenicol. The results of this study indicate the potential risk of infection with Listeria in people consuming raw or undercooked smoked and salted fish. Also, the results obtained in this study indicated the need for appropriate surveillance and epidemiological monitoring strategy to control the development of resistance.  Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  4 - Salmonella contamination of camel meat in various stages of destruction in Isfahan and Chaharmahal va Bakhtiari
  Ebrahim Rahimi Amir Shakeriyan
  Salmonella is a food borne pathogens and pose a public health problem in the world counts. Food poisoning caused by this pathogen with symptoms such as gastroenteritis, diarrhea, abdominal cramps, and fever enteric (typhoid fever) occurs. This study aimed to isolate and More

  Salmonella is a food borne pathogens and pose a public health problem in the world counts. Food poisoning caused by this pathogen with symptoms such as gastroenteritis, diarrhea, abdominal cramps, and fever enteric (typhoid fever) occurs. This study aimed to isolate and identify Salmonella typhimurium in various stages of destruction was carried camel meat. For this purpose, a total of 150 samples of camel meat in various stages of destruction (after peeled, after unloading viscera after the wash), (each 50 samples) in return when summer 1395 to winter 1395 the slaughterhouse city Ben Chaharmahal and Bakhtiari Province and the city of Najaf Abad and Esfahan Golden city, were taken. The results showed that 22 of 150 samples of camel meat as species of Salmonella were detected. Using PCR showed that of the 22 positive samples, 3 (2%) Salmonella typhimurium and 19% (6/12) were other species. From the 50 samples collected after unloading viscera 11 positive cases were detected in 2 (4%) are Salmonella typhimurium. After washing the other 50 samples were collected 4 cases (8%) is related to Salmonella spp and Salmonella typhimurium serotypes 4 none of it. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  5 - Evaluation of Escherichia coli population in intestine and meat of broilers by cinnamon and thyme supplement in chicken diet
  Ahmad Zeinodini Majid Gholami-Ahangaran Ebrahim Rahimi
  For evaluation and comparing effect of thyme and cinnamon on carcass contamination, 135 broiler chicks divided to 3 groups by 3 replicates. All chickens reared under same condition and received 0.5 percent thyme and 0.5 percent cinnamon on basic diet, continuously. Cont More

  For evaluation and comparing effect of thyme and cinnamon on carcass contamination, 135 broiler chicks divided to 3 groups by 3 replicates. All chickens reared under same condition and received 0.5 percent thyme and 0.5 percent cinnamon on basic diet, continuously. Control chickens received no supplement in basic diet. In 42 days old, chickens were slaughtered and breast muscle was sampled for Escherichia coli (E.coli) detection. Cecal content were sampled for E.Coli colony counting, by conventional bacteriologic methods. Results showed that administration of 0.5 percent thyme can reduce carcass contamination and E.coli population in cecal content, significantly. There was no significant difference between control chickens and chickens received cinnamon for percentage of E.coli detection on carcass and cecal Ecoli population. all studied indices. According to results of this study, continuous consumption of 0.5 percent of thyme can improve hygienic quality in broiler chickens while this effect not expected for cinnamon. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  6 - The growth modelling of Escherichia coli in commercial chicken meat extract affecting by temperature and pH factors
  Anna Ghaseminezhad Ali fazara Ebrahim Rahimi
  The purpose of this study is to provide a predictive model of Escherichia coli by selective growth factors such as heat storage (8 ° and 37 ° C) and PH (4, 5 and 6) in the extract was chicken business. For this purpose, a commercial extract of chicken prepared u More

  The purpose of this study is to provide a predictive model of Escherichia coli by selective growth factors such as heat storage (8 ° and 37 ° C) and PH (4, 5 and 6) in the extract was chicken business. For this purpose, a commercial extract of chicken prepared using acetic acid and 1/0 normal profit on the desired PH was adjusted. The level of 105 cfu / ml of the extract prepared from bacteria were inoculated. Extract in the temperature of 8 and 37 ° C and at a different time than the samples were measured. . The results are consistent with the expectation that the significant growth in the PH 4 (P <0/05), less than the growth of the PH 5 and 6, respectively. In contrast to the expected reduction in the temperature of 37 ° C and 8 ° C was observed. In other words, the bacteria grow in temperatures of 8 and 37 were the same. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  7 - The study of Iranian food contact plastic wrap contamination with bacteria causing food-borne disease
  Hajieh Ghasemian Safaei Nasrin Mirzaei Ahmand Reza Bahrami Bayan Saeidi Ebrahim Rahimi
  The use of plastic wrap for foodstuff is very common in the world. Plastic wrap is impermeable to air, oils, greases, and more importantly, bacteria which makes it useful for food packaging. Bacillus cereus and Staphylococcus aureus are of important pathogens and the mo More

  The use of plastic wrap for foodstuff is very common in the world. Plastic wrap is impermeable to air, oils, greases, and more importantly, bacteria which makes it useful for food packaging. Bacillus cereus and Staphylococcus aureus are of important pathogens and the most common cause of food-borne disease.Ten Iranian plastic wraps with different brands were chosen and identification was done by biochemical test and serial dilution methods. After microorganism identification, selective and differential medium cultures were used to confirm bacteria. Among ten Iranian plastic wrap; seven samples were devoid of any contamination, while two samples had Bacillus cereus contamination and one plastic wrap sample had Staphylococcus aureus contamination. According to the result of serial dilution, 1.6X104 CFU and 2.1X102 CFU Bacillus cereus and 1.7X101 CFU Staphylococcus aureus was counted. Acceptable microbial limits for Bacillus cereus and Staphylococcus aureus per gram of plastic wrap need to be established by international organizations for standardization. Besides, the presence of Bacillus cereus and Staphylococcus aureus on plastic wrap surface and related disease in food contact plastic wrap needs to have a continuous investigation. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  8 - Isolation of Campylobacter in different processing stage and presentation of poultry carcasses
  amin irannejhad ebrahim rahimi majid gholamiahangaran
  Campylobacter is one of the main potentials for causing diarrhea and bacterial gastroenteritis in humans worldwide. The aim of this study was to determine the prevalence of campylobacter in poultry carcasses during slaughtering process in Najaf abad-Isfahan slaughterhou More

  Campylobacter is one of the main potentials for causing diarrhea and bacterial gastroenteritis in humans worldwide. The aim of this study was to determine the prevalence of campylobacter in poultry carcasses during slaughtering process in Najaf abad-Isfahan slaughterhouse. In summer 2014, 160 samples were collected from chicken carcasses from 5 chicken flock during 8 different stage of slaughtering and analyzed for detecting campylobacter spp. The results showed that 102 samples from 160 samples (%63.75) were positive for campylobacter. The prevalence of campylobacter in different stage were 19 of 20 (%95) in cloaca, 16 of 20 (%80) predefeathering, 12of 20 (%60) post defaethering, 12 of 20 (%60) post evisceration, 14 of 20 (%70) post chilling, 13 of 20 (%65) post packing, 10 of 20 (%50) post deboning and 6 of 20 (%30) post freezing. Also biochemical differentiation of the produced campylobacteria isolates showed that C.jejuni was frequently isolated (%90.2) than C.coli (%9.8). Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  9 - Prevalence and antimicrobial resistance pattern of Escherichia coli O157: H7 isolated from avian eggs
  mehdi moradi sarmeidani mohsen fardemadi Ebrahim Rahimi abdolkarim zamani moghadam
  Escherichia coli (E. coli) is one of the most common intestinal pathogenic bacteria that cause various infections among men and animals. E. coli O157:H7 is one of the major serotypes which is placed in the group of Enterohemorrhagic E. coli or EHEC. The purpose of this More

  Escherichia coli (E. coli) is one of the most common intestinal pathogenic bacteria that cause various infections among men and animals. E. coli O157:H7 is one of the major serotypes which is placed in the group of Enterohemorrhagic E. coli or EHEC. The purpose of this study was searching for serotype O157:H7 in samples of domestic birds’ egg in the market by culture method and PCR molecular method and the antibiotic resistance of this bacterium was investigated. Totally, 87 samples of domestic poultry eggs (chicken, quail, pigeons, ostriches) three parts of shell, yolk and white (from each part a separate sample) were examined and after culturing steps, 8 samples were infected with this bacterium. Then, these 8 samples were tested with PCR strain and finally, 4 samples had O157: H7 strain genotype. Also after conducting antibiogram tests to determine the sensitivity toward different antibiotics, the most sensitivity of E.coli was belonged to antibiotics ciprofloxacin, ceftiroxone, enrofloxacin, enrofloxacin and nalidixic acid. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  10 - The comparing of antibiotic resistance pattern in Escherichia coli isolates from chicken meat that reared under conventional and without antibiotic condition
  Mohammadreza Shahiri Majid Gholami-Ahangaran Ebrahim Rahimi
  The using of antibiotics in chicken production is one of main concerns in consumers of this protein source that usually these compounds administrated for antimicrobial effect and control of microbial infections in poultry production. For comparing the antibiotic resista More

  The using of antibiotics in chicken production is one of main concerns in consumers of this protein source that usually these compounds administrated for antimicrobial effect and control of microbial infections in poultry production. For comparing the antibiotic resistance pattern in Escherichia coli (E. coli) that isolated from different growing conditions (conventional and without antibiotic), the E. coli strains were isolated from chicken meat and cecal content of chickens that were reared under conventional and without antibiotic conditions, in Isfahan province. After purification of E.coli strains on specific bacterial culture, and approve of bacterial strains according to biochemical tests, the isolates were cultured on Mueller-Hinton culture. The antibiotic resistance pattern was examined by disc-diffusion agar test. The results showed that the antibiotic resistance to different medicinal and veterinary antibiotics groups has occurred in two different growing conditions but the antibiotic resistance was lower in without antibiotic than conventional growing condition. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  11 - Prevalence of antibiotic resistance genes in Entrococcus faecalis isolated from meat in Shahrekord
  Hamed Karimian الهه Tajbakhsh ebrahim rahimi
    In the past two decades, Enterococci resistant to some antibiotics has gained widespread that it would cause complications in the treatment of infections caused by these bacteria. Since meat as a foodstuff consumed in Iran is common and easily contaminated wi More

    In the past two decades, Enterococci resistant to some antibiotics has gained widespread that it would cause complications in the treatment of infections caused by these bacteria. Since meat as a foodstuff consumed in Iran is common and easily contaminated with bacteria therefore, we decided to detect frequency of antibiotic resistance genes in Enterococcus faecalis isolated from meat in Shahrekord. This cross sectional study was conducted on 104 samples of meat in 2016 in Shahrekord. Isolation of bacteria using by biochemical methods and confirmed with detection of 16srDNA gene, and antibiotic resistance genes isolates using by Kirby-Bauer method and molecular method for detection of tetM, ant (2'''')-I, erm B and aac (6'')/aph (2'') genes. Most bacterial resistance to streptomycin (2/95%) and sensitivity to vancomycin (100%) were estimated. Prevalence of ermB gene reported 76.9% and prevalence of aac (6'')/aph (2'') gene reported 40% in E. faecalis isolated. The results indicate that is not resistance to vancomycin in E. faecalis isolated from meat in Shahrekord, but high resistance to Streptomycin, Cefotaxime, Meropenem, Erythromycin, Tetracycline were found also found that ermB gene in E. faecalis high prevalence of antibiotic-resistant gene is the most abundant strength. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  12 - Isolation of Salmonella typhimurium and Salmonella enteritidis in bison milk and dairy products and bison meat by cultural and PCR method
  Sanaz Alizadeh Hassan Momtaz Ebrahim Rahimi
  This study aims at investigating the outbreak of Salmonella and Disease-Agent Genes Pf Salmonella interitidis and Salmonella typhimurium separted from bison milk and its products and bison meat in Khuzestan province- Mollasani town. On the More

  This study aims at investigating the outbreak of Salmonella and Disease-Agent Genes Pf Salmonella interitidis and Salmonella typhimurium separted from bison milk and its products and bison meat in Khuzestan province- Mollasani town. On the Whole, 210 samples of meat, milk and its products in Esfand 94 and Farvardin 95 have been collected from Mollasani town. Samples have been analyzed for the presence of S. interitidis and S. typhimurium and Disease- Agent Genes. The outbreak of Salmonella in the studied sample was 2.8%. Highest out breaks of contamination were respectively in cheese sample 10%, cream 7.14% and 2.5%. No Salmonella was detected in meat, yogurt, butter and rice pudding. The separated Salmonella in the sample were S. interitidis5% and S. typhimurium 2.5%. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  13 - Study the prevalence rate and antibiotic resistance pattern of the Staphylococcus aureus strains isolated from different types of vegetables and salads in Chaharmahal VA Bakhtiari province in Spring, 2017
  Manouchehr Momeni shahraki Amir Shakerian Ebrahim Rahimi
  Staphylococcus aureus is one of the main causative agents of food poisoning in human. Occurrence of severe antibiotic resistances has increased the importance of this bacterium. The present study was carried out to assess the prevalence rate and antibiotic resistance pa More

  Staphylococcus aureus is one of the main causative agents of food poisoning in human. Occurrence of severe antibiotic resistances has increased the importance of this bacterium. The present study was carried out to assess the prevalence rate and antibiotic resistance pattern of S. aureus strains isolated from vegetable and salad samples in Chaharmahal Va Bakhtiari Province. In this study, 485 samples of vegetable and salad were collected and immediately transferred to the laboratory. Samples were evaluated by microbial culture and the isolated S. aureus strains were analyzed for the antibiotic resistance pattern using the disk diffusion method. The prevalence rate of S. aureus in vegetable and salad samples were 16% and 11.91%, respectively. The highest (26.50%) and the lowest (6.72%) contamination rates were observed in Lordegan and Shahrekord cities. A Significant statistical difference was observed for the prevalence of S. aureus between vegetable and salad samples (P<0.05). S. aureus strains isolated from vegetable and salad samples had the highest prevalence of resistance against penicillin (95.58%), tetracycline (88.23%), ampicillin (79.41%), erythromycin (72.05%) and methicillin (70.58%) antibiotics. The lowest prevalence of antibiotic resistance was reported against imipenem (7.35%), chloramphenicol (22.05%), cotrimoxazol (26.47%) and co-amxiclave (29.41%). Judicious prescription of antibiotics according to the results of the disk diffusion method can reduce the risk of resistant S. aureus in vegetable and salad. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  14 - Study the frequency of enterotoxin encoding genes and antibiotic resistance pattern of methicillin-resistant Staphylococcus aureus isolated from vegetable and salad samples in Chaharmahal Va Bakhtiari province
  Manouchehr Momeni shahraki Amir Shakerian Ebrahim Rahimi Farhad Safarpoor Dehkordi
  Methicillin-resistant Staphylococcus aureus (S. aureus) is one of the main emerging causative agents of antibiotic-resistant food poisoning in humans. The bacterium also has the ability to the production of heat resistant enterotoxin. The present study was done to asses More

  Methicillin-resistant Staphylococcus aureus (S. aureus) is one of the main emerging causative agents of antibiotic-resistant food poisoning in humans. The bacterium also has the ability to the production of heat resistant enterotoxin. The present study was done to assess the prevalence rate, antibiotic resistance pattern, and frequency of enterotoxin encoding genes in methicillin-resistant S. aureus strains isolated from vegetable and salad samples. In this study, 485 samples of vegetables and salad were collected and immediately transferred to the laboratory. The microbial culture was used to isolate S. aureus, and methicillin-resistant strains were approved using cefoxitin and oxacillin disks. Antibiotic resistance patterns and frequency of enterotoxin encoding genes were analyzed using disk diffusion and PCR methods, respectively. The prevalence rate of methicillin-resistant S. aureus in vegetable and salad samples were 7.2% and 8.51%, respectively. Methicillin-resistant S. aureus strains had the highest antibiotic resistance against penicillin (100%), co-amoxiclav (100%), ampicillin (100%) and ceftriaxone (100%) antibiotics. The prevalence of resistance against imipenem (10.52%) and chloramphenicol (23.68%) was lower than other antibiotics. SEA (63.15%) and SEB (52.26%) were the most frequently detected enterotoxins in methicillin-resistant S. aureus strains. The simultaneous presence of multiple enterotoxin encoding genes and multiple resistance against several antibiotics in methicillin-resistant S. aureus strains isolated from vegetable, and salad samples represent an important health-related concern in this kind of food samples. Preventing uncontrolled administration of antibiotics can reduce the risk of enterotoxigenic methicillin-resistant S. aureus enterotoxin in vegetables and salads. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  15 - Study the prevalence and antibiotic resistance of Escherichia coli O157 strains isolated from poultry meat in Isfahan in the summer of 2019
  reza mousavi Ebrahim Rahimi Amir Shakerian
  10.30495/jfm.2021.683628
  Poultry meat is considered as one of the sources of Escherichia coli O157. The present study was performed to evaluate the prevalence and antibiotic resistance of Escherichia coli O157 isolates of poultry meat samples presented in Isfahan. In total, 500 meat samples wer More

  Poultry meat is considered as one of the sources of Escherichia coli O157. The present study was performed to evaluate the prevalence and antibiotic resistance of Escherichia coli O157 isolates of poultry meat samples presented in Isfahan. In total, 500 meat samples were collected from diverse poultry samples supplied in Isfahan and evaluated for the presence of Escherichia coli O157 using microbial culture. Suspicious isolates were confirmed by PCR test. The pattern of antibiotic resistance of isolates was investigated using the antibiotic disk diffusion test. Forty-four out of a total of 500 samples (8.80%) were contaminated with Escherichia coli O157. The highest level of contamination was related to duck meat (16%) and the lowest was related to quail meat (3%). There was a statistically significant difference of P Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  16 - Genotypic and Phenotypic Pattern of Antibiotic Resistance of Acinetobacter baumannii Isolated from Traditional Butter and Cream in Isfahan
  Nahal Salimi Mohammad Ahmadi Ebrahim Rahimi
  10.30495/jfm.2022.683636
  Acinetobacter species are saprophytic and have emerged as an important nosocomial pathogen. In this study, 100 samples of traditional butter and cream, were evaluated for the presence of the A. baumannii. The A. baumannii isolates were genotyped based on virulence genes More

  Acinetobacter species are saprophytic and have emerged as an important nosocomial pathogen. In this study, 100 samples of traditional butter and cream, were evaluated for the presence of the A. baumannii. The A. baumannii isolates were genotyped based on virulence genes and phenotype according to antibiotic resistance patterns. The results showed that from 50 samples of butter and cream, 2 samples (4%) and 1 sample (2%) were contaminated with A. baumannii. Antibiotic resistance examination showed that all isolates were resistant to the antibiotics of meropenem, imipenem, chloramphenicol, methicillin, carbapenem and fusidic acid. The most abundant genes encoding antibiotic resistance in A. baumannii strains were tetA, tetB, dfrA1, aac (3) -IV, sul1, cnf2, csgA, jurA, citm, blasHV, aadA1 and Aac3IV. The results also showed that the most abundant virulent genes in A. baumannii strains that detected from traditional milk and dairy products were fimH, papC, Pai and kpsmTII, respectively. It is recommended to use a preventive method to reduce or eradicate A. baumannii from the human food chain and to prevent the spread of infection. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  17 - Prevalence of Pseudomnas aeruginosa in various species of shrimps of Isfahan and Chabahar in summer and autumn and the effect of season and place of supply on it
  Seyedmajid Hashemi Ebrahim Rahimi Seyed Amirali Anvar Hamed Ahari Maryam Ataee
  10.30495/jfm.2022.1955955.1772
  Pseudomonas aeruginosa a potential risk factor for food poisoning especially in aquatic foods like shrimp. In order to determine and compare the rate of infection in different species of shrimp in two seasons of summer and autumn in Isfahan and Chabahar cities, the abdo More

  Pseudomonas aeruginosa a potential risk factor for food poisoning especially in aquatic foods like shrimp. In order to determine and compare the rate of infection in different species of shrimp in two seasons of summer and autumn in Isfahan and Chabahar cities, the abdominal muscle of 35 shrimps in each season of each city were sampled. Samples were homogenized in peptone water medium, then incubated and cultured in PCA medium. Suspected colonies were isolated and then confirmatory tests of Pseudomonas aeruginosa and confirmation of the presence of nanI, the specific gene of Pseudomonas aeruginosa by PCR.In summer, in Isfahan and Chabahar, 48.5% and 25.7% were infected respectively. In autumn, the prevalence was 22.9% for Isfahan and 20% for Chabahar, respectively. In Isfahan, the highest rate of infection was related to Metapenaueus affinis and the lowest to Penaeus semisulcatus in summer. In autumn, the highest number of infected samples was related to Penaeus merguiensis and the lowest to Penaeus semisulcatus. In Chabahar city, in both seasons, the most infected samples were related to Metapenaeus affinis, and the lowest to Panaeus semisulcatus and in autumn was related to both Penaeus semisulcatus and Litopenaeus vannamei.There was a high prevalence of this bacterium in samples of different shrimp species in both seasons and both cities, which indicates a high risk of food poisoning of Pseudomonas aeruginosa due to improper consumption of shrimp. Cold chain observance during storage, transportation and sell of shrimp will be very effective in controlling this problem. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  18 - Frequency of binding genes in Staphylococcus aureus isolated from dairy products in Shahrekord. Binding genes of Staphylococcus aureus in dairy products
  Rasul Pajohesh Elahe Tajbakhsh Hassan Momtaz Ebrahim Rahimi
  10.30495/jfm.2022.1971880.1795
  Staphylococcus aureus is an opportunistic pathogen in humans and animals, which can cause contaminating dairy products. In this research, 250 traditional dairy products, including butter, yogurt, cheese, curd, and cream, were prepared from different parts of Shahrekord More

  Staphylococcus aureus is an opportunistic pathogen in humans and animals, which can cause contaminating dairy products. In this research, 250 traditional dairy products, including butter, yogurt, cheese, curd, and cream, were prepared from different parts of Shahrekord city and transported to the microbiology laboratory of Shahrekord Azad University in sterile containers with ice. The isolation of Staphylococcus aureus bacteria was done according to standard protocols and molecular methods. Genetic evaluation of biofilm was done by PCR method to detect the most common biofilm coding genes (icaA, icaB, icaC, icaD, bap, fnbA, fnbB, clfB, clfA). From the total of 250 examined samples, 50 samples (20%) were positive for Staphylococcus aureus. Contamination was reported in 26% of curd, 24% of butter, 22% of cheese, 16% of cream, and 12% of yogurt. The frequency of the mentioned genes was reported as 74%, 68%, 64%, 76%, 30%, 96%, 90%, 32% and 28% respectively. In the microtiter plate method, biofilm reaction was observed in 30 isolates (60%), strong biofilm was observed in 20 isolates (66.66%), and weak biofilm was observed in 10 isolates (33.33%). No biofilm reaction was observed in 5 isolates (16.66%). Based on Fisher's exact test, a statistically significant relationship was reported between the studied genes and strong and moderate biofilm reaction. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  19 - Investigating the antioxidant properties of grape seed extracts of Iranian varieties and evaluating the antimicrobial effect on pathogenic pathogens isolated from poultry
  Sayed Ahmad Nourbakhsh Ebrahim Rahimi
  10.30495/jfm.2023.1991519.1826
  The aim of the present study is to investigate the antioxidant and antimicrobial properties of hydro alcoholic and aqueous extracts of grape seed varieties on Gram-positive and Gram-negative microorganisms isolated from poultry meat. 94 poultry meat samples including ch More

  The aim of the present study is to investigate the antioxidant and antimicrobial properties of hydro alcoholic and aqueous extracts of grape seed varieties on Gram-positive and Gram-negative microorganisms isolated from poultry meat. 94 poultry meat samples including chicken, quail, turkey and ostrich were randomly collected from sales centers of Isfahan province. Microorganisms were identified by biochemical tests. Aqueous and hydro alcoholic extracts of grape seeds of black alfi, black gohari, black shahani, Rish-Baba, Shasat-arus and Rooster tail varieties were prepared by soaking method. Antimicrobial properties of hydro alcoholic and aqueous extracts of grape seeds were investigated by disk diffusion and MIC (minimum inhibitory concentration) methods. The antioxidant property of the extracts was evaluated by DPPH method and the IC50 value was calculated. The phenolic compounds present in aqueous and hydro alcoholic extracts of grape seeds were measured by high-performance liquid chromatography. The frequency of Arcobacter butzleri, Campylobacter jejuni, Listeria monocytogenes and Staphylococcus aureus was 11.45%, 17.70%, 1.04% and 16.66%, respectively. The antimicrobial effect of aqueous and alcoholic extracts of grape seed was higher on gram positive bacteria in both methods. Alcoholic extracts had more antimicrobial effect than aqueous extracts, Rish-Baba grape showed the most antimicrobial effect. The highest percentage of DPPH in hydro alcoholic extracts is related to black alfi and Rish-Baba grape seed extracts, and in aqueous extracts of black alfi and black shahani grape seeds. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  20 - Prevalence of Staphylococcus aureus in meat and meat products
  Fatemeh Nonahal Ebrahim Rahimi Esmail Ataie Salehi
  Staphylococcus aureus as the second or third important food-borne pathogen all around the world. Ability of development and production of heat-stable enterotoxin in a wide range of the food made  Staphylococcus has one of the most important factors causing the food More

  Staphylococcus aureus as the second or third important food-borne pathogen all around the world. Ability of development and production of heat-stable enterotoxin in a wide range of the food made  Staphylococcus has one of the most important factors causing the food poisoning. This study was done to investigates the prevalence of Staphylococcus in meat and  meat products  in  Esfahan. From summer to winter 2012, 450 samples including  raw beef (n=80), minced meat (n=80), lamb (n=80), goat (n=80), camel (n=50), hamburger (n=40), kebab (n=40) were randomly collected from butcheries and supermarkets  in Isfahan, and analyzed for the presence of S. aureus. Totally, 234 (55/6 %) S. aureus were detected in meat and meat products. The mean count of S. aureus in positive samples was 8/3×102. The results indicated that although the percentage of contaminated  samples was high, The number of bacteria presented in the samples is low with no potential risk for public health. To effectively manage the food production for decreasing diseases more epidemiologic investigations about S. aureus toxication are needed. Manuscript profile