List of Articles

• Open Access Article
  • Abstract Page
  • Full-Text
  
  1 - Identification of virulence genes in serotype O:3 Yersinia enterocolitica isolated from turkey meat by PCR method in Shahrekord
  Atieh Hadad elahe tajbakhsh Maryam Reiysi
  Nowadays are being used PCR-based methods in order to detect Yersinia enterocolitica strains and plasmids and chromosomal virulence genes. This method able to provide a rapid and reliable characterization. In this study, 300 samples of turkey meat were collected randoml More

  Nowadays are being used PCR-based methods in order to detect Yersinia enterocolitica strains and plasmids and chromosomal virulence genes. This method able to provide a rapid and reliable characterization. In this study, 300 samples of turkey meat were collected randomly from market in Shahrekord. The isolates were identified using PCR test, And the virulence genes of the isolates were detected. According to the results, 55 serotypes O:3 were identified, and the prevalence of virulence genes were as follows: yadA (32.72%), inv (100%), ail (38.18%), ystA (40%), and virF (27.27%). Due to the increased consumption of turkey meat in the country and the possibility of Y. enterocolitica transfer to humans, it is recommended to carefully observe storage principles, avoid use of undercooked turkey meat and also applying PCR test for direct identification of contamination in food as a quick, accurate and specific technique is recommended. Key words: PCR method, Turkey meat, Virulence genes, Yersinia enterocolitica Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  2 - The comparing of antibiotic resistance pattern in Escherichia coli isolates from chicken meat that reared under conventional and without antibiotic condition
  Mohammadreza Shahiri Majid Gholami-Ahangaran Ebrahim Rahimi
  The using of antibiotics in chicken production is one of main concerns in consumers of this protein source that usually these compounds administrated for antimicrobial effect and control of microbial infections in poultry production. For comparing the antibiotic resista More

  The using of antibiotics in chicken production is one of main concerns in consumers of this protein source that usually these compounds administrated for antimicrobial effect and control of microbial infections in poultry production. For comparing the antibiotic resistance pattern in Escherichia coli (E. coli) that isolated from different growing conditions (conventional and without antibiotic), the E. coli strains were isolated from chicken meat and cecal content of chickens that were reared under conventional and without antibiotic conditions, in Isfahan province. After purification of E.coli strains on specific bacterial culture, and approve of bacterial strains according to biochemical tests, the isolates were cultured on Mueller-Hinton culture. The antibiotic resistance pattern was examined by disc-diffusion agar test. The results showed that the antibiotic resistance to different medicinal and veterinary antibiotics groups has occurred in two different growing conditions but the antibiotic resistance was lower in without antibiotic than conventional growing condition. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  3 - Investigating effect of pH and concentration of strawberry juice on the viability of Lactobacillus casei and Lactobacillus plantarum
  Tahereh Berimavandi Vajiheh Fadaei Noghani
  In this studyresearch, probiotic strawberry juice was produced using probiotic bacteria Lactobacillus casei and Lactobacillus plantarum and four factors brix (9,11,13), pH (3,4), time (0,7,14,21,28) and bacteria (Lactobacillus casei and Lactobacillus plantarum) were stu More

  In this studyresearch, probiotic strawberry juice was produced using probiotic bacteria Lactobacillus casei and Lactobacillus plantarum and four factors brix (9,11,13), pH (3,4), time (0,7,14,21,28) and bacteria (Lactobacillus casei and Lactobacillus plantarum) were studied using factorial design in a completely randomized factorial design; and some physicochemical properties (pH, acidity, vitamin C and soluble solids) and probiotic bacterial population of probiotic strawberry juice were measured. The result showed that during storage time, pH, vitamin C, total soluble solids and bacterial population decreased (p<0.05) and acidity increased (p<0.05). With increasing the brix, acidity, soluble solids and bacterial population increased (p<0.05) and vitamin C decreased (p<0.05). With increasing pH, Vitamin C and bacterial population increased (p<0.05) and acidity decreased (p<0.05). The results showed Lactobacillus casei was higher; also, the highest viability of probiotic bacteria in pH = 4, brix= 13 and storage time zero was observed. Keywords: Strawberry, Lactobacillus casei, Lactobacillus plantarum, probiotic juice Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  4 - شناسنامه علمی شماره
  
  Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  5 - Effect of lemon grass extract on shelf life and quality properties of minced beef during stored at 4°C
  Mahnaz Hashemiravan Mehrdad Karimi majd Ali Reza Shahab Lavasani
  Abstract The use of natural extracts, are suitable as a source of antioxidants, to improve the quality of beef is increasing. In this study, extracts of lemon grass after the preparation and evaluation and formulation of Beef with MBC and inhibitory concentrations of 2 More

  Abstract The use of natural extracts, are suitable as a source of antioxidants, to improve the quality of beef is increasing. In this study, extracts of lemon grass after the preparation and evaluation and formulation of Beef with MBC and inhibitory concentrations of 250, 500 and 1000 ppm concentrations of MIC and MBC on days zero, 10th, 20th and 30th maintenance was evaluated. Properties were evaluated total count of microorganisms, Escherichia coli population count, pH, sensory analysis (taste, texture, chewiness and overall acceptability), colorimetry (index of brightness, red, yellow), the moisture percentage of total volatile nitrogen the results showed that by increasing the use of lemon grass extract microbial index and total volatile nitrogen significantly reduced during storage. But indicators of brightness and reduce redness and yellowness index is also significantly reduced. Significantly increase the moisture content and the characteristics of taste and texture, chewiness and also features in amounts of 500 ppm lemon grass were prpoposed. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  6 - Production of beta-galactosidase from whey using Kluyveromyces marxianus
  nahid ebrahimi Fatemeh Nejati
  Beta-galactosidase is one of the most important industrial enzymes capable of decomposing lactose into glucose and fructose. In this study, two strains of Kluyveromyces marxianus (GY101 and BY101) were investigated for the production of the β-galactosidase from whe More

  Beta-galactosidase is one of the most important industrial enzymes capable of decomposing lactose into glucose and fructose. In this study, two strains of Kluyveromyces marxianus (GY101 and BY101) were investigated for the production of the β-galactosidase from whey as a culture media. Whey was inoculated with each strain and examined for enzyme production with aeration at 100 rpm for 144 hours. In order to increase the enzyme production, the effect of adding yeast extract (0.2 and 0.5%), magnesium sulfate (0.5%) and manganese sulfate (0.5%) at 24, 48, 96, 120 and 144 hours at 30°C and 37°C were investigated and the enzyme activity was measured. The highest enzyme production after 144 hours at 30°C with GY101 and BY101 strains by adding yeast extract (0.5%) were 2.25 and 2.18 U/ml, and with magnesium sulfate added 2.12 and 1.94 U/ml, respectively. According to the results of this study, whey cheese is an appropriate environment for the production of the β-galactosidase enzyme using Kluyveromyces marxianus with the presence of yeast extract and magnesium and manganese sulfate as supplements. The best temperature for the production of the enzyme in both strains was 30°C, and the addition of mineral supplements and yeast extract had a positive effect on the production of β-galactosidase. While the GY101 strain was more suitable than BY101 and magnesium sulfate was more suitable than manganese sulfate, and the enzyme activity increased with increasing yeast extract concentration. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  7 - Prevalence and antimicrobial resistance pattern of Escherichia coli O157: H7 isolated from avian eggs
  mehdi moradi sarmeidani mohsen fardemadi Ebrahim Rahimi abdolkarim zamani moghadam
  Escherichia coli (E. coli) is one of the most common intestinal pathogenic bacteria that cause various infections among men and animals. E. coli O157:H7 is one of the major serotypes which is placed in the group of Enterohemorrhagic E. coli or EHEC. The purpose of this More

  Escherichia coli (E. coli) is one of the most common intestinal pathogenic bacteria that cause various infections among men and animals. E. coli O157:H7 is one of the major serotypes which is placed in the group of Enterohemorrhagic E. coli or EHEC. The purpose of this study was searching for serotype O157:H7 in samples of domestic birds’ egg in the market by culture method and PCR molecular method and the antibiotic resistance of this bacterium was investigated. Totally, 87 samples of domestic poultry eggs (chicken, quail, pigeons, ostriches) three parts of shell, yolk and white (from each part a separate sample) were examined and after culturing steps, 8 samples were infected with this bacterium. Then, these 8 samples were tested with PCR strain and finally, 4 samples had O157: H7 strain genotype. Also after conducting antibiogram tests to determine the sensitivity toward different antibiotics, the most sensitivity of E.coli was belonged to antibiotics ciprofloxacin, ceftiroxone, enrofloxacin, enrofloxacin and nalidixic acid. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  8 - Antibiotic resistance determination in Escherichia coli isolated from poultry samples enffected Colibacillosis disease from around Kermanshah farms
  Forogh Mohammadi
  Abstract Poultry colibacillosis is one of the most important diseases which is caused by Escherichia coli and cause great economic Damages to chicken farm industry. According to high spread of antibiotic resistance in this bacterium and create resistance strain of Esche More

  Abstract Poultry colibacillosis is one of the most important diseases which is caused by Escherichia coli and cause great economic Damages to chicken farm industry. According to high spread of antibiotic resistance in this bacterium and create resistance strain of Escherichia coli, this study conducted to detect antibiotic resistance profile in Escherichia coli isolated from Colibacillosis in chicken carcasses transferred to Veterinary Diagnosis laboratories in Kermanshah, Iran. Antibiotic resistance profile was studied in 500 isolated of Escherichia coli in spring and summer of 2014 using simple disk diffusion method. The results showed that the first quarter of year had mostly Escherichia coli resistance to Chloretetracycline, Erythromycin, Oxytertracyclin and Colistin and in summer in addition of this four antibiotics, Danfloxacin had high rate of resistance antibiotic. According to the increase spread of antibiotic resistance, it is necessary to prevent of illness entrance to the poultry with proper nutrition principles and hygiene. Timely vaccination can prevent greatly of chicken farm with infectious diseases and follow that, the rate of antibiotic using is low and we can development into products without antibiotics. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  9 - Contamination rate and biofilm formation by psychotrophic bacteria from bulk milk tanks and dairy processing equipments
  Rasoul Rezapour Shahram Hanifian
  Psychorotrophs are the heterogeneous group of bacteria that can grow at refrigerator temperatures. Most of these bacteria produce extracellular proteases and lipases which decompose lipids and proteins in milk that leads to undesirable defect in milk. These defects do n More

  Psychorotrophs are the heterogeneous group of bacteria that can grow at refrigerator temperatures. Most of these bacteria produce extracellular proteases and lipases which decompose lipids and proteins in milk that leads to undesirable defect in milk. These defects do not disappear even after pasteurization and sterilization. This study aimed to investigate the occurrence rate and diversity of psychotropic bacteria in raw milk tankers and dairy processing equipments as well as the biofilm-forming ability of the isolates. A total of 80 samples including 30 samples of raw milk tanks, 30 samples of dairy product processing equipments and 20 samples from different surfaces of the production line were collected. According to the results, 26.25% of the samples were found contaminated with psychrotrophic bacteria. Amongst, 65% of the surface samples of production line, 23.33% of the raw milk tanks and 3.3% of the processing equipment were positive for psychrotrophic bacteria. Out of 31 isolates, Bacillus genus had the highest contamination rate (32.25%), whilst Alcaligenes showed the lowest rate (3.2%). The results of biofilm formation revealed that 21 isolates (67.75%) were able to produce biofilm. It was concluded that in some cases, current CIP procedure is not efficient for the entire removal of biofilm from the processing equipments. It seems that it is necessary to apply complementary physical/chemical approaches along with the current CIP procedure to complete obliteration of biofilms from dairy industry. Manuscript profile