List of Articles Elahe Tajbakhsh

• Open Access Article
  • Abstract Page
  • Full-Text
  
  1 - Typing of the Acinetobacter baumannii strains isolated from row meat of poultry and livestock using Multi Locus Sequence Typing (MLST)
  Marziyeh Tavakol Hassan Momtaz Parviz Mohajeri Leili Shokoohizadeh Elahe Tajbakhsh
  Acinetobacter baumannii (A. baumannii) strains with multiple drug resistance are mainly opportunistic pathogens in the development of hospital infections and as an emerging contaminant in livestock-based foods. The aim of this study was to determine the antibiotic resis More

  Acinetobacter baumannii (A. baumannii) strains with multiple drug resistance are mainly opportunistic pathogens in the development of hospital infections and as an emerging contaminant in livestock-based foods. The aim of this study was to determine the antibiotic resistance pattern and genotyping of this bacterium strains in raw meat of poultry and livestock. 22 strains isolated from raw meat were tested by multi-locus sequence typing and simple disk diffusion methods. The highest antimicrobial resistance was observed to tetracycline with 90.9% and the least antibiotic resistance was azithromycin and imipenem with 9.09%. Five strains were identified as non-typing isolates in 22 isolates of A. baumannii. Five genetic profiles (Sequence Types=ST) including ST15, ST10, ST12, ST25, ST25 were identified. Identifying the acceptable level of genetic variation among isolates using the MLST technique indicates that this method is considered as a useful method in the study and typing of Acinetobacter spp. strains and can be strains isolated from different origins in different groups. In this study, it was found that by sequencing of house-keeping genes, it is possible to typing of Acinetobacter spp. strains, and this amount of polymorphism indicates that this technique is a useful method for analyzing the genetic diversity of A. baumannii strains is a source of animal origin. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  2 - Prevalens of entrocins produced by Entrococcus fecalis isolated from dairy products in Shahrekord city
  Elaheh Barzam الهه تاج بخش Ebrahim Rahimi
  Bacteriocins are proteins produced by bacteria especially Acid lactic bacteria having antimicrobial characteristics and used for food preservation. In recent years, especial considerations have been taken to detection of acid lactic bacteria and Bacteriocins in food ind More

  Bacteriocins are proteins produced by bacteria especially Acid lactic bacteria having antimicrobial characteristics and used for food preservation. In recent years, especial considerations have been taken to detection of acid lactic bacteria and Bacteriocins in food industries to use them as food preservatives. In this study, 120 samples of different traditional dairy products were examined to investigate genes of Enterocin, Entrain A, Enterocin P and Enterocin AS-48. After separation and detection of bacteria in presence of special primers related to Enterocingenes, their frequencies were studied. Among 120 samples, 44 (36.36%) were contaminated to Enterococcusfireclays. The gene related to EnterocinA in 14 isolates (31/81%), the gene related to Enterocin P in 13 isolates (29/54%), the gene related to EnterocinAs-48 in 9 isolates (20/45%), the simultaneous presence of the gene related to EnterocinA and P in 2 isolates (4/54%), the gene related to Enterocins A and As-48 in 7 isolates (15/09%) and the gene related to Enterocins A, P and As-48 in 5 isolates (11/36%) were observed. According to the presence of a lot of Enterocingenes in Enterococcusfaecalis separated from dairy products, doing research on antimicrobial characteristics of Enterocins produced by this bacterium is of great necessity.  Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  3 - Identification of virulence genes in serotype O:3 Yersinia enterocolitica isolated from turkey meat by PCR method in Shahrekord
  Atieh Hadad elahe tajbakhsh Maryam Reiysi
  Nowadays are being used PCR-based methods in order to detect Yersinia enterocolitica strains and plasmids and chromosomal virulence genes. This method able to provide a rapid and reliable characterization. In this study, 300 samples of turkey meat were collected randoml More

  Nowadays are being used PCR-based methods in order to detect Yersinia enterocolitica strains and plasmids and chromosomal virulence genes. This method able to provide a rapid and reliable characterization. In this study, 300 samples of turkey meat were collected randomly from market in Shahrekord. The isolates were identified using PCR test, And the virulence genes of the isolates were detected. According to the results, 55 serotypes O:3 were identified, and the prevalence of virulence genes were as follows: yadA (32.72%), inv (100%), ail (38.18%), ystA (40%), and virF (27.27%). Due to the increased consumption of turkey meat in the country and the possibility of Y. enterocolitica transfer to humans, it is recommended to carefully observe storage principles, avoid use of undercooked turkey meat and also applying PCR test for direct identification of contamination in food as a quick, accurate and specific technique is recommended. Key words: PCR method, Turkey meat, Virulence genes, Yersinia enterocolitica Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  4 - Prevalence of antibiotic resistance genes in Entrococcus faecalis isolated from meat in Shahrekord
  Hamed Karimian الهه Tajbakhsh ebrahim rahimi
    In the past two decades, Enterococci resistant to some antibiotics has gained widespread that it would cause complications in the treatment of infections caused by these bacteria. Since meat as a foodstuff consumed in Iran is common and easily contaminated wi More

    In the past two decades, Enterococci resistant to some antibiotics has gained widespread that it would cause complications in the treatment of infections caused by these bacteria. Since meat as a foodstuff consumed in Iran is common and easily contaminated with bacteria therefore, we decided to detect frequency of antibiotic resistance genes in Enterococcus faecalis isolated from meat in Shahrekord. This cross sectional study was conducted on 104 samples of meat in 2016 in Shahrekord. Isolation of bacteria using by biochemical methods and confirmed with detection of 16srDNA gene, and antibiotic resistance genes isolates using by Kirby-Bauer method and molecular method for detection of tetM, ant (2'''')-I, erm B and aac (6'')/aph (2'') genes. Most bacterial resistance to streptomycin (2/95%) and sensitivity to vancomycin (100%) were estimated. Prevalence of ermB gene reported 76.9% and prevalence of aac (6'')/aph (2'') gene reported 40% in E. faecalis isolated. The results indicate that is not resistance to vancomycin in E. faecalis isolated from meat in Shahrekord, but high resistance to Streptomycin, Cefotaxime, Meropenem, Erythromycin, Tetracycline were found also found that ermB gene in E. faecalis high prevalence of antibiotic-resistant gene is the most abundant strength. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  5 - Identification of Methicillin-Resistant Staphylococcus epidermidis (MRSE) Strains Associated with Food Poisoning Outbreaks in Clinical Samples
  Parisa Behshod Elahe Tajbakhsh Hassan Momtaz
  Methicillin-Resistant Staphylococcus epidermidis is an important pathogen that causes infectious diseases whose treatment is extremely formidable. Staphylococcus epidermidis enterotoxins with effects on intestinal epithelial cells can are be causing Create food poisonin More

  Methicillin-Resistant Staphylococcus epidermidis is an important pathogen that causes infectious diseases whose treatment is extremely formidable. Staphylococcus epidermidis enterotoxins with effects on intestinal epithelial cells can are be causing Create food poisoning in people. The aim of current study is to the identification of MRSE strains associated with food poisoning outbreaks in Isfahan. During six- months, 60 clinical specimens to isolated from strains of Staphylococcus epidermidis were screened. Following identification strains, MRSE isolates were isolated by PCR method and, and then antibiotic resistance pattern of them was determined by Kirby – Bauer method. The presence of the sea, seb, sed and, sei genes was analyzed by PCR. 45 isolates of Staphylococcus epidermidis were isolated from 60 samples, 30 isolate (66.6 percent) were MRSE. MRSE isolates exhibited the highest rates of resistance to penicillin (80 percent), and cefoxitin (56.6 percent), while they showed the lowest resistance to levofloxacin (13.3 percent), and rifampicin (6.6 percent). The prevalence rate of Moreover, the frequency of enterotoxin genes sea, seb, sed and, sei was 60 percent, 63.3 percent, 13.3 percent and, 76.6 percent respectively, in the isolate. In this study, high percentage of MRSE isolates were antibiotic resistant and produced enterotoxin. Considering that these toxins are superantigen and can more intense the complications of clinical and nosocomial infections, detecting and rapid treatment of these infections are essential. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  6 - Genotyping of Escherichia coli O157: H7 strains isolated from raw ruminant and poultry meat samples by RAPD-PCR
  Mandana Lotfi Hassan Momtaz Elahe Tajbakhsh
  Escherichia coli O157:H7 is one of the most important enteric pathogenic strains that is usually transmitted to humans through contaminated water and food. These strains cause hemorrhagic colitis, HUS syndrome, cytopenic purpura thrombosis, and, in some cases, death. Th More

  Escherichia coli O157:H7 is one of the most important enteric pathogenic strains that is usually transmitted to humans through contaminated water and food. These strains cause hemorrhagic colitis, HUS syndrome, cytopenic purpura thrombosis, and, in some cases, death. The main repository of these bacteria is ruminants. The aim of this study was genetic classification (genotyping) of isolates of this strain isolated from raw meat and poultry by RAPD-PCR method. 344 samples of ruminants and poultry were collected from fresh meat supply centers in Isfahan and Shahrekord. Genetic classification of O157:H7 isolates was performed by the RAPD-PCR method. Of 344 raw meat samples studied, 202 Escherichia coli isolates (58.7%) were isolated. The rate of O157: H7 strain was 17.8% (36 samples). Genetic classification of Escherichia coli O157:H7 isolates showed 9 different profiles among these 36 isolates and found an affinity of 43.7 to 100% among the isolates. This study showed high molecular similarity between E. coli O157: H7 strains isolated from different animals in Isfahan and Shahrekord. It was also found that RAPD-PCR is a simple, fast, and inexpensive method for describing the genetic diversity of different E. coli strains including strain O157:H7. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  7 - Occurrence and antibiotic resistance of Enterococcus faecalis strains isolated from fish and shrimp caught from the Persian Gulf
  Neda Noroozi Hassan Momtaz Elahe Tajbakhsh
  10.30495/jfm.2022.1938722.1730
  The present study was performed to evaluate the occurrence and pattern of antibiotic resistance of Enterococcus faecalis strains isolated from fish and shrimp samples caught from the Persian Gulf. A total of 240 seafood samples caught from the Persian Gulf, including 12 More

  The present study was performed to evaluate the occurrence and pattern of antibiotic resistance of Enterococcus faecalis strains isolated from fish and shrimp samples caught from the Persian Gulf. A total of 240 seafood samples caught from the Persian Gulf, including 120 fish and 120 shrimp samples were collected from Bushehr province. The presence of E. faecalis was confirmed using microbial culture and biochemical tests. The pattern of antibiotic resistance of E. faecalis isolates was evaluated using Disc Diffusion Method. Fifty-four out of 240 (22.50%) samples were contaminated with E. faecalis. The contamination of E. faecalis in fish and shrimp samples were 30% and 15 percent, respectively. E. faecalis strains isolated from fish and shrimp samples had the highest resistance to gentamicin (100%), tetracycline (100%), erythromycin (100%), cefazolin (90.74%) and trimethoprim-sulfamethoxazole (88.88%) antibiotics. Resistance to chloramphenicol was not observed in any of the isolates. The results of the study showed that fish and shrimp can be considered as possible sources of antibiotic-resistant E. faecalis. Therefore, complete cooking of seafood before consumption, observance of hygiene in fishing and sale centers and prescribing antibiotics according to the results of the disk diffusion test can prevent gastrointestinal infections caused by antibiotic-resistant strains of E. faecalis. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  8 - Prevalence of virulence factors in Enterococus faecalis isolated from hamburger
  Razeyeh Mehdeyan الهه Tajbakhsh zahra bamzaheh
  10.30495/jfm.2021.683624
  Enterococci are microorganisms that are mainly found in the intestines. Their high presence in food can be a cause of fecal contamination and they are isolated from various foods such as: vegetables, meat, etc. Among the food, hamburger is one of the products that is us More

  Enterococci are microorganisms that are mainly found in the intestines. Their high presence in food can be a cause of fecal contamination and they are isolated from various foods such as: vegetables, meat, etc. Among the food, hamburger is one of the products that is used by hand and widely by people. In this study, 50 hamburger samples were examined for biochemical and molecular methods for the presence of Enterococcus faecalis. In order to detect virulence genes in the presence of specific primer pairs, PCR reaction temperature program was performed. In this study, out of 50 samples, 33 samples (66%) were infected with E. faecalis. Of 33 E. faecalis isolates isolated from hamburgers, efaA reported in 8 isolates (24.24%), cylA reported in 15 isolates (45.45%), gel E reported in 9 isolates (27.27%), esp reported in 2 isolates (6.06%), agg reported in 2 isolates (6.06%), and aca, asa and cyl B were not observed in any of the isolates. Enterococci and coliforms are considered as two important health indicators. Considering that the high presence of enterococci in food can be a reason for fecal contamination, the results of this study indicate high contamination of burgers offered in Shahrekord city. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  9 - Comparison of antibiotic resistance and virulence factors in Enterococcus faecalis isolates isolated from animal and human sources
  Mahmoud Shahveh Elahe Tajbakhsh Hassan Momtaz Reza Ranjbar
  Enterococci are an important and diverse group of bacteria that are known to be resistant to most antibiotics used to treat diseases. In this cross-sectional study, 104 samples of red meat and 1000 urine samples suspected of urinary tract infection in the border city of More

  Enterococci are an important and diverse group of bacteria that are known to be resistant to most antibiotics used to treat diseases. In this cross-sectional study, 104 samples of red meat and 1000 urine samples suspected of urinary tract infection in the border city of Kermanshah were examined for Enterococcus faecalis. First, the samples were approved by biochemical and molecular methods, then in order to evaluate their ability to produce biofilm, Microtiter Plate method was used and their sensitivity to antibiotics was also determined by Kirby-Bayer method. Enterococcus faecalis infection in human samples and red meat samples was reported to be 5% and 40.38% respectively. In the strains isolated from red meat samples, the highest resistance was reported to be to Streptomycin while the lowest resistance was to Vancomycin. In the human isolate samples, the highest resistance was reported to be to Co-trimoxazole ,while the lowest resistance was to Nitrofurantoin . In strains isolated from red meat, ebp A, ebp B and ebp C were reported to be 71.43%, 59.52% and 64.28% respectively. No statistically significant relationship was observed between biofilm production and ebp genes in these isolates. However, in strains isolated from urine, a significant relationship was detected between ebp genes and biofilm production. Similarly, it was reported that there was no statistically significant relationship between the meat type and the virulence gene type. But, the findings of the study showed a significant relationship between the frequency of efa A, gel E, ace and esp genes. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  10 - Frequency of binding genes in Staphylococcus aureus isolated from dairy products in Shahrekord. Binding genes of Staphylococcus aureus in dairy products
  Rasul Pajohesh Elahe Tajbakhsh Hassan Momtaz Ebrahim Rahimi
  10.30495/jfm.2022.1971880.1795
  Staphylococcus aureus is an opportunistic pathogen in humans and animals, which can cause contaminating dairy products. In this research, 250 traditional dairy products, including butter, yogurt, cheese, curd, and cream, were prepared from different parts of Shahrekord More

  Staphylococcus aureus is an opportunistic pathogen in humans and animals, which can cause contaminating dairy products. In this research, 250 traditional dairy products, including butter, yogurt, cheese, curd, and cream, were prepared from different parts of Shahrekord city and transported to the microbiology laboratory of Shahrekord Azad University in sterile containers with ice. The isolation of Staphylococcus aureus bacteria was done according to standard protocols and molecular methods. Genetic evaluation of biofilm was done by PCR method to detect the most common biofilm coding genes (icaA, icaB, icaC, icaD, bap, fnbA, fnbB, clfB, clfA). From the total of 250 examined samples, 50 samples (20%) were positive for Staphylococcus aureus. Contamination was reported in 26% of curd, 24% of butter, 22% of cheese, 16% of cream, and 12% of yogurt. The frequency of the mentioned genes was reported as 74%, 68%, 64%, 76%, 30%, 96%, 90%, 32% and 28% respectively. In the microtiter plate method, biofilm reaction was observed in 30 isolates (60%), strong biofilm was observed in 20 isolates (66.66%), and weak biofilm was observed in 10 isolates (33.33%). No biofilm reaction was observed in 5 isolates (16.66%). Based on Fisher's exact test, a statistically significant relationship was reported between the studied genes and strong and moderate biofilm reaction. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  11 - Determining antibiotic resistance pattern in Enterobacter cloacae strains isolated from checken and sheep meat in Shahrekord city
  elahe barzam الهه تاج بخش Hassan Momtaz
  Usually, contaminated food is one of the main causes of human infections, and in this case, poultry meat and sheep are considered as the main causes. Enterobacter cloacae strains, having different virulence factors and multiple antibiotic resistance are mainly considere More

  Usually, contaminated food is one of the main causes of human infections, and in this case, poultry meat and sheep are considered as the main causes. Enterobacter cloacae strains, having different virulence factors and multiple antibiotic resistance are mainly considered as an opportunistic pathogen. In this research, the isolation of Enterobacter cloacae from chicken and sheep meat samples in Shahrekord in 2019 was done by microbial and molecular methods. Antibiotic resistance pattern was determined by disc diffusion method and microtitre plate method was used to check biofilm production. The ability to produce broad-spectrum β-lactamase enzymes was investigated through phenotypic and genotypic methods. Out of 384 examined samples, Enterobacter cloacae were identified in 25 samples (6.51%) which also confirmed in the presence of the hsp60 in molecular analysis. Among these, 18 samples were related to chicken meat (72%) and 7 samples (28%) were related to sheep meat. The highest antibiotic resistance to cotrimoxazole and cefotaxime was reported in 20 isolates (80%) and the lowest resistance to nitrofuranthein was reported in 15 isolates (23.8%). In microtiter method. 15 isolates (60%) showed strong biofilm reaction, 10 isolates (40%) showed moderate biofilm reaction. The present study indicates that ESBL-producing Enterobacter cloacae strains have a relatively high prevalence. The increase in the number of these strains is often caused by the irrational prescription of antibiotics, which requires the use of new antimicrobial agents,. Manuscript profile