Journal of Food Microbiology: 5 (4)

Open Access Article

1 - Study of frequency of NHE complex genes in Bacillus cereus isolated from cow milk in Tabriz city
Taher Rasoulpour Saman Mahdavi

Bacillus cereus is a gram positive and spore producing bacterium that is widely spread in nature. Many of this bacterium strains cause food poisoning with signs of diarrhea and vomiting. Diarrhea-type diseases cause by haemolytic enterotoxins HBL, non haemolytic NHE and More

Bacillus cereus is a gram positive and spore producing bacterium that is widely spread in nature. Many of this bacterium strains cause food poisoning with signs of diarrhea and vomiting. Diarrhea-type diseases cause by haemolytic enterotoxins HBL, non haemolytic NHE and cytotoxin K. Milk is one of the food products that may be contaminated by livestock with mastitis caused by Bacillus cereus. The aim of this research was the study of frequency of NHE complex genes in Bacillus cereus isolated from cow milk in Tabriz city. 42 samples of Bacillus cereus isolated from cow milk after molecular confirmation were investigated for study of frequency of NHE complex genes by use of specific primers by PCR method. Of 42 tested samples, 41 positive cases were detected. Of 41 Bacillus cereus tested, 37 cases (90.2%), 35 cases (85.3%) and 31 cases (75.6%) harbored nheA, nheB and nheC genes, respectively. 30 samples (73.1%) simultaneously contained all three studied genes. In 3 samples (7.3%) of Bacillus cereus bacteria, no NHE complex genes were observed. The simultaneous presence of NHE complex genes in most of tested Bacillus cereus isolates indicates the high virulence of tested bacteria and their high pathogenic potential. Manuscript profile

Open Access Article

2 - Effect of Plant Extracts and Packaging on Control of Microbial Property and Antioxidant Activity of Basil
narjes mansouri Mohammad Moghaddam fatemeh kazemi masomeh bahraini Hussain Aroiee

In order to disinfection and increase the shelf life of vegetables, it is necessary to use natural compounds with suitable packaging. In this study, natural plant compounds were studied in reducing microbial load and their effect, along with two types of zip-plast and c More

In order to disinfection and increase the shelf life of vegetables, it is necessary to use natural compounds with suitable packaging. In this study, natural plant compounds were studied in reducing microbial load and their effect, along with two types of zip-plast and cellophane packaging on total phenolic content and antioxidant activity of basil. Hydro-alcoholic extracts of green tea and Khorasani thyme were prepared. Basil leaves were exposed to concentrations of 10, 20 and 40 percent of the extracts for 15 minutes. Then they were placed in the packaging of cellophane and zip-plast and storage with a relative humidity of 85% and a temperature of 7°C. Reduced bacterial count was measured after basil treatment by the different concentrations mentioned in both extracts. Total phenolic content and antioxidant activity basil were measured during the storage period for four periods. 40% concentration of green tea extract reduced the Enterobacteriaceae by 4.48 logarithms in basil samples. Extract concentration of 40% Khorasan thyme and green tea were able to bring coliform bacteria population to zero. In the survey of total phenolic content of basil during storage showed 10% concentration of Khorasan thyme extract at zip-plast packaging and in antioxidant activity of basil samples, 10% concentration of both extracts in zip-plast packaging had the least changes compared to fresh basil. In addition, green tea and Khorasan thyme extracts as natural compounds with high antioxidant and antimicrobial properties, along with zip-plast packaging, can be considered for increasing the shelf-life and reducing the microbial pollution of basil Manuscript profile

Open Access Article

3 - Using micellar casein concentrate (MCC) on efficiency and physicochemical properties of Iranian white brine cheese
somayeh Aghaei Ali Reza Shahab Lavasani peyman Rajaei

White brined cheese is a very popular traditional cheese which is produced in some parts of Iran, this products is usually made from sheep,s, cow,s milk or mixture of two. Back to past several decades, one of the most challenge was increased yield of cheesemaking accord More

White brined cheese is a very popular traditional cheese which is produced in some parts of Iran, this products is usually made from sheep,s, cow,s milk or mixture of two. Back to past several decades, one of the most challenge was increased yield of cheesemaking according to improved quality including chemical composition and sensory properties. So, the aims of this study were to determine physicochemical and sensory properties of white brined cheese supplemented by Micellar Casein Concentrate (MCC). Five treatments were prepared by adding 0,1,2,3 and 4% of MCC to milk used for cheesemaking and physicochemical properties (pH, acidity(dornic), dry matter%, moisture%, ash%, protein%, fat% and yield of cheesemaking) also sensory properties(flavor, color, texture and acceptability scores) were measured over 30 days of ripening period. Statistical analysis for comparison of data was Completely Randomized Design(CRD). Results showed that in parallel to dry matter%, moisture content of all treatments were decreased during 30 days of ripening period and parallel to pH, acidity of all treatments increased until end of ripening period also ash% of all treatments increased during 30 days of ripening period and the highest yield of cheesemaking , protein% and fat% were attributed to T4(containig 4% of MCC). The highest scores for flavor, color, texture and acceptability were attributed to T4(containig 4% of MCC). According to above mentioned, T1 was the best treatment among others and had high similarity to control sample with due attention to physicochemical and sensory properties. Manuscript profile

Open Access Article

4 - Scientific- Winter 1398

Manuscript profile

Open Access Article

5 - Evaluation of Medicinal infusions signs in cattle slaughtered at the abattoir Sari
mohsen yadollahi Nasrollah Vahedi Nouri Yazdan Khodabakhsh

Summary: Today, Antibiotics are mainly used to treat and also stimulate the growth of livestock in the diet. In the event of uncontrolled consumption of drugs especially antibiotics and lack of observance Contraception time (Withdrawal time), Causes harmful effects such More

Summary: Today, Antibiotics are mainly used to treat and also stimulate the growth of livestock in the diet. In the event of uncontrolled consumption of drugs especially antibiotics and lack of observance Contraception time (Withdrawal time), Causes harmful effects such as: Bacterial resistance, allergic reactions, poisoning, Carcinogenicity and disturb the natural microflora of the intestines in the body of the consumer. This study was conducted to determine the rate of injection of drugs and its effects on cows' carcasses. This study is descriptive-cross sectional. Within six months, From June to December 2016, a total of 8630 cow carcasses were surveyed from Sari industrial slaughterhouse. For this purpose, carcasses were examined (Effects of Injection, Smell the drug odor) daily at the slaughterhouse. Of the 8630 carcasses examined, 1,500 carcasses (17.38 %) had injectable effects. The results of the research show that there the difference between males and females in terms of the effects of drug injections is statistically significant in both summer and autumn (P&lsaquo;0.05).But between summer and autumn, the difference is not statistically significant(P&rsaquo;0.05). Manuscript profile

Open Access Article

6 - Study of the effect of incorporation of Lactococcus lactis on the shelf life of rainbow trout fillet in glacial condition
Asad Abbaspour Anbi Vadood Razavilar Moslem Neyriz Naghadehi Yoseif Ali Asadpour Osalou

The present study was conducted to evaluate the effect of Lactococcus lactis and its supernatant inclusion in order to increase the shelf-life of rainbow trout fillets stored in the refrigerator. Forty-five pieces of cultured rainbow trout with a total weight of 30 kg w More

The present study was conducted to evaluate the effect of Lactococcus lactis and its supernatant inclusion in order to increase the shelf-life of rainbow trout fillets stored in the refrigerator. Forty-five pieces of cultured rainbow trout with a total weight of 30 kg were prepared and were immersed in solutions of L. lactis with a dilution of 106 CFUml-1 and its supernatant with percentages of 30 and 60. In order to monitor the corruption procedure of fillet samples, chemical, microbial and sensory factors were analyzed on days 0, 5, 10 and 15. Control fillet samples had the highest and samples of fillets treated with pure bacteria contained the lowest number of microorganisms (P ≤0.05). The mean log of the number of psychrophilic, psychotrophic, mesophilic bacteria, yeast and mold per each gram of fillet was significantly lower than that of control (P ≤0.05). The effect of lactic acid bacteria and supernatant on the reduction of thiobarbituric acid and total volatile nitrogen at the end of storage time was significant (P ≤0.05) but were not significant on pH changes (P >0.05). The use of pure bacteria increased the points given to sensory factors. Samples treated with pure bacteria and supernatants were in good condition in term of taste at the end of the maintenance period while other treatments were unusable. Considering the results, L. lactis and its supernatant is suggested improving the shelf life of rainbow trout fillets. Manuscript profile

Open Access Article

7 - Effect of myofibrillarProtein in combination with Zataria multiflora essential oil on increasing the shelflife of fish fillet of Otolithes ruber
fatemeh fathimoghadam laleh roomiani

In this study, the effect of myofibrilar protein with the Zataria essential oil was investigated in three concentrations of 0.5, 1 and 1.5% on the shelf life of fish fillets in a period of 15 days at a refrigerated temperature. According to the analysis of essential oil More

In this study, the effect of myofibrilar protein with the Zataria essential oil was investigated in three concentrations of 0.5, 1 and 1.5% on the shelf life of fish fillets in a period of 15 days at a refrigerated temperature. According to the analysis of essential oil, the dominant components were carrocolol (46.82%), thymol (18.34%) and linalool (12.71%). There was no significant difference between the treatments with control and treatment of myofibrilear protein film and essential oil of Shirzi. During 15 days of storage, the parameters of TBA (Thiobarbituric Acid), PV (Peroxide), FFA (Free Fatty Acids), TVB-N (Total Volatile Nitrogen Bases) showed an increase in the concentration of thyme essential oil in myofibrilear film decreased these parameters . Accordingly, the myofibricular protein film with 1.5% essential oil of essential oil of thyme with a significant difference compared with the control had the highest efficiency in increasing the shelf life of the fish fillet. Color index L * and a * did not change significantly during storage (P Manuscript profile

Open Access Article

8 - High tetracycline resistance alarm in Iran
sahar nouri Mohammad Nodargah

The bacterial resistance to antibiotics have become a worldwide health concern. Tetracycline has not been excluded, its low price and broad-spectrum antimicrobial agent activity have led to high consumption in the aspects of health care and food hygiene. Escherichia col More

The bacterial resistance to antibiotics have become a worldwide health concern. Tetracycline has not been excluded, its low price and broad-spectrum antimicrobial agent activity have led to high consumption in the aspects of health care and food hygiene. Escherichia coli is an indicator of bacterial species in food, human, and livestock health, which has led to a good model for studying antibiotic resistance. The purpose of this study was to evaluate the E.coli strains tetracycline resistance status in food, human and animal populations of Iran up to 2018. The discussion about food is important as a common area of human, animal populations and environment and also as one of the resistant strain transmission factors. A survey of the study’s results showed that the average resistance to Tetracycline in poultry with Colibacillosis 86/74 percent, human isolates 64/11 percent and food isolates are 60/9 percent. Regarding this level of resistance, it is recommended that the distribution and consumption of tetracycline in Iran should be discontinue for a period of time in order to reduce the population of resistant strains by other antibiotics and methods. The molecular epidemiology and affecting factors of resistance creation should be evaluate. Manuscript profile

Open Access Article

9 - Fish-borne parasites: A review on the reports from Iran
Hossein Momeni Mehdi Raissy Masoumeh Bashiri Maryam Barzegar Mahsa Ansari

A high number of parasites can infect fish, and some can be transmitted to humans. Human or fish-eating mammals can be the main host of these parasites. Today, more than 40 species of fish-borne parasites have been identified, causing disease in humans. These parasites More

A high number of parasites can infect fish, and some can be transmitted to humans. Human or fish-eating mammals can be the main host of these parasites. Today, more than 40 species of fish-borne parasites have been identified, causing disease in humans. These parasites can be classified as protozoa, trematode, Cestoda, Nematoda and Acanthocephala. Infection in humans usually occurs following the consumption of larvae-infected meat. The possibility and the rate of infection directly relate to different criteria, including the fish consumption rate, level of hygiene, feeding habits, and methods of cooking or processing. In this article, having discussed the fish-borne parasites, the reports of zoonotic parasites from fish in Iran have been discussed. Manuscript profile

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