Usually, contaminated food is one of the main causes of human infections, and in this case, poultry meat and sheep are considered as the main causes. Enterobacter cloacae strains, having different virulence factors and multiple antibiotic resistance are mainly considere
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Usually, contaminated food is one of the main causes of human infections, and in this case, poultry meat and sheep are considered as the main causes. Enterobacter cloacae strains, having different virulence factors and multiple antibiotic resistance are mainly considered as an opportunistic pathogen. In this research, the isolation of Enterobacter cloacae from chicken and sheep meat samples in Shahrekord in 2019 was done by microbial and molecular methods. Antibiotic resistance pattern was determined by disc diffusion method and microtitre plate method was used to check biofilm production. The ability to produce broad-spectrum β-lactamase enzymes was investigated through phenotypic and genotypic methods. Out of 384 examined samples, Enterobacter cloacae were identified in 25 samples (6.51%) which also confirmed in the presence of the hsp60 in molecular analysis. Among these, 18 samples were related to chicken meat (72%) and 7 samples (28%) were related to sheep meat. The highest antibiotic resistance to cotrimoxazole and cefotaxime was reported in 20 isolates (80%) and the lowest resistance to nitrofuranthein was reported in 15 isolates (23.8%). In microtiter method. 15 isolates (60%) showed strong biofilm reaction, 10 isolates (40%) showed moderate biofilm reaction. The present study indicates that ESBL-producing Enterobacter cloacae strains have a relatively high prevalence. The increase in the number of these strains is often caused by the irrational prescription of antibiotics, which requires the use of new antimicrobial agents,.
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