XML
-
-
List of Articles
-
Open Access Article
1 - Prevalence and antibiotic resistance of meat of various birds infected with Arcobacter in sales centers of Isfahan province in four seasons of 2018
roya najafi ebrahim rahimi Amir ShakerianThe genus Arcobacter, along with Campylobacter, belongs to the family Campylobacteriaceae. Arcobacteria are gram-negative, non-spore-forming, microaerophilic bacteria that can be distinguished from Campylobacter genus by growing in the presence of oxygen and at low temp MoreThe genus Arcobacter, along with Campylobacter, belongs to the family Campylobacteriaceae. Arcobacteria are gram-negative, non-spore-forming, microaerophilic bacteria that can be distinguished from Campylobacter genus by growing in the presence of oxygen and at low temperatures.These rod-shaped bacteria are S-shaped or spiral. Arcobacter butzleri is the most common species of this genus; it is known as an emerging zoonotic pathogen. The main aim of the present research was to evaluate the prevalence of Arcobacter butzleri in the poultry meat sold in the sales centers of Isfahan province.More specifically, this study examined different types of poultry meat for their potential infection with Arcobacter species. Accordingly , the samples were collected in Isfahan province during four seasons; then they were transferred with ice to the Food Quality Control Laboratory of Islamic Azad University, Shahrekord branch.For the isolation of Arcobacter species, 10 g of each homogenized sample of meat was transferred to Arcobacter selective broth (ASB), which included the selected CAT supplement , and incubated for 2-4 days at 42 ° C; then the blood agar was cultured linearly in specific Arcobacter media. The plates were incubated for 2-4 days at the temperature of 42 ° C under aerobic conditions.Typical colonies were cultured in Brucella Agar medium under the same conditions; then the pure cultures were evaluated in terms of their phenotypic characteristics, catalase activity and nitrate reduction. To confirm the diagnosis and differentiation of Arcobacter species, DNA extraction was carried out by applying a DNA extraction kit Manuscript profile -
Open Access Article
2 - Summer 2022
-
Open Access Article
3 - Effect of Bunium Persicum Essential Oil,, NaCl and , Bile Salts on viability of Lactobacillus-acidophilus (ATCC4356)
Zhaleh KhoshkhoThe viability of Lactobacillus acidophilus in the commercial preparation process and its usage as probiotic Bactria is useful. The main purpose of this paper is to study the effect of Bunium persicum essential oil (BEO) stress, NaCl, bile salts, and their combinations o MoreThe viability of Lactobacillus acidophilus in the commercial preparation process and its usage as probiotic Bactria is useful. The main purpose of this paper is to study the effect of Bunium persicum essential oil (BEO) stress, NaCl, bile salts, and their combinations on the viability of Lactobacillus acidophilus Bactria. In the following, the effect of BEO stress (0.5 percent), Nacl (2 percent), bile salt (0.15 percent) in comparison with the treatment instance was studied on the Lactobacillus acidophilus population in the refrigerator. Furthermore, the data analysis process was done with the two-way ANOVA and comparing the means by LSD method in SAS software version 9. The conclusions have been out coming from three-time tests with the consideration of P<0.05 in all test steps. After four weeks of 4-Celsius degree temperature conditions, the viability of Bactria and PH decreased, while the amount of degradation in the treated sample was less than the index in the stressed samples. Moreover, the amount of acidity raised in all samples and this growth in stressed samples was more than a treatment sample (P< 0.05). As a conclusion, using BEO with NaCl and bile salt in the densities below of MIC can lead to the viability of Bactria till the end of 14th-day maintenance in the probiotic product. Manuscript profile -
Open Access Article
4 - Study the antimicrobial effects of ethanolic extract of Thymus vulgaris on foodborne bacteria in vitro
Meysam Alimoradi Mehrdad Ataie Kachoie Fariborz MoattarThymus vulgaris is a plant of family Lamiaceae that has high antimicrobial properties. The present study was performed to evaluate the antimicrobial effects of the ethanolic extract of Thymus vulgaris on food-borne bacteria. Aerial part of Thymus vulgaris was used to pr MoreThymus vulgaris is a plant of family Lamiaceae that has high antimicrobial properties. The present study was performed to evaluate the antimicrobial effects of the ethanolic extract of Thymus vulgaris on food-borne bacteria. Aerial part of Thymus vulgaris was used to prepare methanolic extract. The diameter of the growth inhibition zone of bacteria was assessed using disk diffusion. Minimum inhibitory concentration (MIC) and Minimum Bacterial Concentration (MBC) of ethanolic extract of Thymus vulgaris were evaluated on the target bacteria using ELISA plate. The diameter of the growth inhibition zone of bacteria against the ethanolic extract of Thymus vulgaris ranged from 7.87±0.61 to 16.99±0.37 mm. Application of 50 mg/ml concentration of ethanolic extract of Thymus vulgaris caused the highest diameter of the growth inhibition zone of Pseudomonas aeruginosa (11.38±0.43 mm), Escherichia coli (12.00±0.35 mm), Staphylococcus aureus (16.33±0.69 mm) and Listeria monocytogenes (16.99±0.37 mm). The antimicrobial effects of ethanolic extract of Thymus vulgaris were dose-dependent (P <0.05). The diameter of growth inhibition zone of 50 mg/ml of ethanolic extract of Thymus vulgaris on Pseudomonas aeruginosa, Staphylococcus aureus and Listeria monocytogenes was higher than all antibiotics (P <0.05). The lowest MIC and MBC values were obtained for Staphylococcus aureus (4 and 6 mg/ml, respectively) and Listeria monocytogenes (4 and 6 mg/ml, respectively). Thymus vulgaris ethanolic extract can be used as a combination with antimicrobial properties in food. Manuscript profile -
Open Access Article
5 - Prevalence of Cysticercus bovis infection in cattle slaughtered in Isfahan and Shahrekord slaughterhouses by molecular and microscopic methods
Seyed Reza Hosseini Shahin NejatCysticercus bovis is a neonatal stage of stenosis called Taenia saginata. Laboratory diagnosis is based on the appearance of the parasite and the microscopic examination of the pathology. Misdiagnosis occurs when other parasites, such as Sarcocystis, are present or when MoreCysticercus bovis is a neonatal stage of stenosis called Taenia saginata. Laboratory diagnosis is based on the appearance of the parasite and the microscopic examination of the pathology. Misdiagnosis occurs when other parasites, such as Sarcocystis, are present or when the parasite is metamorphosed and calcified. Therefore, more accurate tests are necessary to diagnose the parasite. In this study, observational and cross-sectional analysis and diagnosis were performed using PCR and primer method based on mitochondrial cytochrome c oxidase gene. To investigate the occurrence of cows infected with Cysticercus bovis in Isfahan and Shahrekord, 384 samples of thigh and heart muscle tissues of cows slaughtered in Isfahan and Shahrekord slaughterhouses were tested for Cysticercus bovis infection by molecular methods. Based on the tests performed, 3.64% of the samples were infected with Cysticercus bovis. The results of this study showed that infection of bovine carcasses with Cysticercus bovis is significant and this could increase the incidence of adult worms in the human population. Manuscript profile -
Open Access Article
6 - Evaluation of Tetracycline Antibiotic Level in Raw Milk using Infrared Fourier Transform Spectrum
Ali Ghanbari Afshin Akhoondzadeh-BastiDue to the consumption of milk in the daily diet of humans as one of the main food groups, the health of this product is of particular importance. In this study, FTNIR and FTMID were used to evaluate the amount of tetracycline in raw milk and compared with the reference MoreDue to the consumption of milk in the daily diet of humans as one of the main food groups, the health of this product is of particular importance. In this study, FTNIR and FTMID were used to evaluate the amount of tetracycline in raw milk and compared with the reference HPLC method. Forty milk samples were prepared with different concentrations of tetracycline 0, 50, 100 and 150 ppb. Also, by comparing the control sample with the samples containing tetracycline, the most changes in the near-infrared spectrum occur in the wavelength range of 5200 to 5000 and 7000 to 6800 and the changes of the middle infrared spectrum occur in the wavelength range of 700 to 600, 1700 to 1500 and 3400 to 3200 cm-1. Using these predictions, the amount of tetracycline was predicted by NIR and MIR methods with correlation coefficients of 0.99 and 0.90 and RMSEP error rates of 18.60 and 20.06, respectively. Although the correlation coefficient of both methods is high, but the error rate of the prediction method is high for both methods, especially the MIR method. But the results of the NIR spectrum are closer to the reference method (HPLC) and can replace this method. Due to the lower error rate of NIR and the advantages of this identification method compared to High Performance Liquid Chromatography, this method can be introduced as a suitable alternative to destructive methods of measuring tetracycline in milk. Manuscript profile -
Open Access Article
7 - Evaluation of probiotic properties of Enterococcus faecalis and Enterococcus faecium species isolated from Pousti cheese of Gilan province in 2020
Nasrollah Aali Jalil KhandaghiPousti cheese is a traditional dairy product in different parts of our country. Enterococci are among the predominant bacteria in raw milk cheeses, such as pousti cheeses, which are even used in cheese as a starter or probiotic. The aim of this study was to isolate and MorePousti cheese is a traditional dairy product in different parts of our country. Enterococci are among the predominant bacteria in raw milk cheeses, such as pousti cheeses, which are even used in cheese as a starter or probiotic. The aim of this study was to isolate and identify Enterococcus faecalis and Enterococcus faecium strains with probiotic potential from Pousti cheeses in Gilan province. For this purpose, after isolation and detection of enterococci by phenotypic and biochemical methods, Survival under in-vitro conditions simulating the human GI tract (acid, pepsin, bile and pancreatin), antagonistic properties of isolates and their Auto-aggregation properties, and Co-aggregation with Escherichia coli and Salmonella enterica were evaluated. The results showed the presence of enterococci in 23 cheese samples (76.6%) which were confirmed by confirmational and biochemical tests as 29 isolates of Enterococcus faecium (50%) and 22 isolates of Enterococcus faecalis (37.9%). Totally, 14 samples (27.4%) were able to survive in the presence of acid and pepsin and were selected for the further steps. Finally, 12 isolates well tolerated the presence of bile and pancreatin and their antimicrobial and aggregation properties were evaluated. The isolates showed significant anti-listeria effects and also had a high auto-aggregation power (53.95%) and their co-aggregation effect was higher with Salmonella enterica than Escherichia coli. From the results it can be concluded that some native isolates of Enterococcus faecalis and Enterococcus faecium in Pousti cheese have good potential for use as probiotics. Manuscript profile -
Open Access Article
8 - Evaluation of the effect of Salvia chorassanica essential oil nanoemulsion on reducing mold decay of strawberry fruit after harvest
Mohsen Vazifedoost Azam mehraban zohreh didar Mohammad Hossein Haddadkhodaparast Masoumeh Mehraban Sang AtashIn recent years, due to the harmful effects of toxins and chemical preservatives on human health, the tendency to use natural antimicrobial compounds such as essential oils have been increased. In this study, nanoemulsion of Salvia chorassanica essential oil was prepare MoreIn recent years, due to the harmful effects of toxins and chemical preservatives on human health, the tendency to use natural antimicrobial compounds such as essential oils have been increased. In this study, nanoemulsion of Salvia chorassanica essential oil was prepared by ultrasound method and its antifungal activity against three molds of strawberry rot (A. niger, B. cinere and R. stolonifer) was investigated and compared with thiabendazole fungicide. In addition, the potential of these compounds to reduce mold decay of strawberry fruit during 12 days refrigerated after coating was evaluated. The results showed that the nanoemulsion of essential oil had more antifungal activity and the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of the essential oil nanoemulsion were lower than the thiabendazole. Nanoemulsion treatment of essential oil at a concentration of 12.5 µl / ml on the surface of strawberries was able to delay the growth of A. niger spores for 6 days and B.cinere ، R. stolonifer spores for 9 days. Also, application of nanoemulsion coating of S. chorasanica essential oil and thiabendazole fungicide with a concentration of 25 µl / ml on strawberry surface, unlike control samples without coating, were showed no mold growth until the end of the 12th day. Therefore, nanoemulsion of S. chorasanica essential oil can be suggested as a suitable alternative to the fungicide thiabendazole. Manuscript profile -
Open Access Article
9 - Effect of Olive Leaf and Oregano supplement on performance, oxidation and microbial flora of breast broiler chickens
Neda Sadeghi ronizi seamak parsaei vahid mohammadiThe purpose of this experiment was to investigate the effect of olive leaf and oregano supplement on performance, oxidation and microbial flora of breast broiler chickens. For this, 400 one-day-old Ross 308 broilers were used in a completely randomized design with 5 tre MoreThe purpose of this experiment was to investigate the effect of olive leaf and oregano supplement on performance, oxidation and microbial flora of breast broiler chickens. For this, 400 one-day-old Ross 308 broilers were used in a completely randomized design with 5 treatments and 4 replications. Treatments include; control, 5 g olive leaf, 5 g oregano, 5 g mixture of olive leaf and oregano and 50 g erythromycin . At 42 day of age, four birds from each treatment were randomly selected and euthanized by cervical dislocation. Finally, carcass characteristics were evaluated. To determine oxidation by measuring the concentration of malondialdehyde and for the number of breast meat bacteria per unit colony formation per gram of meat, samples were extracted from the breast tissue of the chickens and stored at 4 ° C. The results showed that a significant reduction in FCR was observed in the birds fed ERY compared to Control group (P < 0.04). Relative breast weight in control birds was low compared to other treatments (P <0.002). Lipid oxidation showed a significant difference between treatments on days 4, 6, 8, 10 and 12 during storage in the refrigerator (P < 0.05). Also, the erythromycin bacteria in breast meat up to day 8 of storage in the refrigerator was affected by the experimental treatments (P <0.05). Overall, the use of a mixture of 5 g of olive leaf and oregano improves the quantity and quality of broiler chickens by reducing the oxidation and TVC bacteria. Manuscript profile
-
The rights to this website are owned by the Raimag Press Management System.
Copyright © 2021-2024