Journal of Food Microbiology: 2 (2)

Open Access Article

1 - Inhibitory effect of Allium sativum L. extract on saprophytic and toxigenic fungi isolated from mayonnaise
Mohammadali Zia Mohammad Goli Ardeshir Ziaee

For its flavoring, prophylactic, and medicinal qualities, Allium sativum is one of the most common plants used in foods. Food poisoning is caused by fungi that grow on foods. The goal of this study was to see whether Allium sativum extracts had antifungal efficacy again More

For its flavoring, prophylactic, and medicinal qualities, Allium sativum is one of the most common plants used in foods. Food poisoning is caused by fungi that grow on foods. The goal of this study was to see whether Allium sativum extracts had antifungal efficacy against saprophytic and toxigenic fungi isolated from contaminated mayonnaise sauce. The mayonnaise-grown fungi were moved to sabouraud's dextrose agar supplemented with chloramphenicol, and macroscopic and microscopic characteristics were used to identify them. The extracts' inhibitory effects against all of the targeted strains studied in the experiment were measured using disc and well diffusion techniques, as well as the minimum inhibitory concentration of each extract. Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus, Penicillium sp., Mucor sp., Rhizopus sp., Cladosporium sp., Fusarium sp., Alternaria sp., and Geotrichum sp. were found. The aqueous extract with the greatest antifungal activity was collected at a concentration of 300 g/ml. Fusarium (33.2 mm) and A. niger (25.2 mm) had the highest and lowest inhibition zones, respectively. Aquatic extract's MIC was estimated to be 350 g/ml for A. niger, A. fumigatus, A. flavus, Mucor sp., Rhizopus sp., Penicillium sp., and Alternaria sp., and 300 g/ml for Cladosporium sp., Fusarium sp., and Geotrichum sp. The MIC of methanol and ethanol extracts was greater than that of aqueous extracts. Manuscript profile

Open Access Article

2 - Summer 2021

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Open Access Article

3 - Source of the Escherichia Coli O157: H7 Outbreak in Yazd Students -2017
ali akbar tajfirouzeh SEYYED MOHAMMAD HOSSEIN HOSSEINI seyyed mohammad javad nezamalhosseini mohsen mehdizadeh shahi HAMID AGHABAGHERI mohammad hassan lotfi seyyed alireza pourmazar alireza talebi

The outbreak of E.coli O157: H7 has been confirmed in human specimens in the spring of 1396 in one of the universities of Yazd. This study was designed to determine the source of infection. This is a case-control study in which 80 patients and 80 healthy students were e More

The outbreak of E.coli O157: H7 has been confirmed in human specimens in the spring of 1396 in one of the universities of Yazd. This study was designed to determine the source of infection. This is a case-control study in which 80 patients and 80 healthy students were examined. The formal validity and content of the questionnaire used was confirmed by the experts and the outbreak team. For data analysis, binary logistic regression and odds ratios with 95% confidence intervals were by SPSS-19 software. In this study, cantaloupe syrup with the odds ratio of 47.37 had the highest factor in the incidence of disease. In the next stage, dinner and rice dinner with a chance ratio of 4.25 and 3.12 respectively, remained significantly in the model. Due to the high chance of cantaloupe syrup in this study and the available records such as laboratory confirmation of the presence of Escherichia coli O157: H7 in human samples and the report of the outbreak team on the preparation of cantaloupe syrup using a sitting meat grinder and also confirm similar studies in Possibility of presence Escherichia coli in raw foods such as meat, it can be concluded that the source of this outbreak was cantaloupe syrup contaminated with Escherichia coli O157: H7 via meat grinder.Keywords: Outbreak, Escherichia coli, Shiga toxin, O157: H7, Yazd. Manuscript profile

Open Access Article

4 - Optimization of the Extraction Process of Aqueous and Alcoholic (Ethanol) Extracts of Allium Ampelloprasuml Plant by Response Surface Methodology and Its Antioxidant Effect on Soybean Oil Stability over Shelf Life
الهام Azadfar Maryam Sabetghadam زهره Bahrami بیتا Beyzaei

Plants contain phenolic compounds ranging from simple molecules such as phenolic acids to highly polymerized molecules such as tannins Phenols and polyphenols in plant tissues have gained increasing scientific interest because of their potential beneficial effects on hu More

Plants contain phenolic compounds ranging from simple molecules such as phenolic acids to highly polymerized molecules such as tannins Phenols and polyphenols in plant tissues have gained increasing scientific interest because of their potential beneficial effects on human health..In this study, Allium ampelloprasum L plant was prepared and homogenized from Sabzevar. The extraction was performed by the ultrasound extraction method using ethanol and water solvent. The amount of and antioxidant compounds of extracts was investigated by and DPPH methods, respectively. The results of the optimization process have shown that the optimum conditions of alcoholic extraction were determined as 17.81 minutes with the concentration of 800. Also, the optimum conditions for the aqueous extraction were determined as the concentration of 800 ppm and the time of 13.52 minutes. According to the optimization results, the extraction amount of antioxidant compounds in aqueous and alcoholic extractions were reported as 33.893-33.499, respectively. The results of the oxidative stability of oil have shown that the peroxide and thiobarbituric acid index in water and alcoholic extraction were 1.938-3.158 and 0.173-0.337, respectively. Manuscript profile

Open Access Article

5 - Comparison of antibiotic resistance and virulence factors in Enterococcus faecalis isolates isolated from animal and human sources
Mahmoud Shahveh Elahe Tajbakhsh Hassan Momtaz Reza Ranjbar

Enterococci are an important and diverse group of bacteria that are known to be resistant to most antibiotics used to treat diseases. In this cross-sectional study, 104 samples of red meat and 1000 urine samples suspected of urinary tract infection in the border city of More

Enterococci are an important and diverse group of bacteria that are known to be resistant to most antibiotics used to treat diseases. In this cross-sectional study, 104 samples of red meat and 1000 urine samples suspected of urinary tract infection in the border city of Kermanshah were examined for Enterococcus faecalis. First, the samples were approved by biochemical and molecular methods, then in order to evaluate their ability to produce biofilm, Microtiter Plate method was used and their sensitivity to antibiotics was also determined by Kirby-Bayer method. Enterococcus faecalis infection in human samples and red meat samples was reported to be 5% and 40.38% respectively. In the strains isolated from red meat samples, the highest resistance was reported to be to Streptomycin while the lowest resistance was to Vancomycin. In the human isolate samples, the highest resistance was reported to be to Co-trimoxazole ,while the lowest resistance was to Nitrofurantoin . In strains isolated from red meat, ebp A, ebp B and ebp C were reported to be 71.43%, 59.52% and 64.28% respectively. No statistically significant relationship was observed between biofilm production and ebp genes in these isolates. However, in strains isolated from urine, a significant relationship was detected between ebp genes and biofilm production. Similarly, it was reported that there was no statistically significant relationship between the meat type and the virulence gene type. But, the findings of the study showed a significant relationship between the frequency of efa A, gel E, ace and esp genes. Manuscript profile

Open Access Article

6 - Evaluation of health status, chemical parameters and organoleptic properties of some nomadic dairy products in Chaharmahal and Bakhtiari province
mohsen bagheri ahmadreza shaviklo

This study aimed to determine the health status, chemical parameters and organoleptic properties of some nomadic dairy products in Chaharmahal and Bakhtiari province. Referring to the nomads of the province, three products including Curd, Gharaghorot and Gee were sample More

This study aimed to determine the health status, chemical parameters and organoleptic properties of some nomadic dairy products in Chaharmahal and Bakhtiari province. Referring to the nomads of the province, three products including Curd, Gharaghorot and Gee were sampled. Sensory experimentation included perfume, flavors, texture, color and overall acceptance. The amount of protein, fat, salt, moisture, ash and some minerals and the E.Coli, Staphylococcus aureus, coliforms, mold and yeast contamination and total microorganisms count in the samples were studied. The amount of protein(P Manuscript profile

Open Access Article

7 - Evaluation of survival of probiotic bacterium Lactobacillus acidophilus (La-5) microencapsulated in synbiotic ketchup sauce and simulated gastric and intestinal environment
mohsen vazifedoost

Tomato ketchup is a widely used product in the world and in Iran, therefore, preparing symbiotic products from it will promote health. In this study, a completely randomized design and response surface method (rotating central composite design) and Design Expert softwar More

Tomato ketchup is a widely used product in the world and in Iran, therefore, preparing symbiotic products from it will promote health. In this study, a completely randomized design and response surface method (rotating central composite design) and Design Expert software (version 7) and Duncan test were used to compare the means. According to the results, the best treatments among the sauce samples in terms of survival of the probiotic bacterium Lactobacillus acidophilus after 30 days of storage, the sample contained 2% lactulose and 2.5% fiber (whether encapsulated or free bacteria). Bacterial residue at the end of storage period was 4.09 *107 cfu/ml in the sauce sample containing encapsulated bacterium and 1.19 * 107 cfu/ml in the sauce sample containing free bacterium. Electron imaging of optimal specimens showed that dimensions of encapsulated bacterium were larger than those of the sauce containing the free bacteria. The transfer of optimal samples to the simulated environment of the stomach and intestines indicated that the sample of the sauce contained encapsulated bacteria in the formulation of which 2.5% fiber and 2% lactulose were used, having a significant difference (p Manuscript profile

Open Access Article

8 - Prevalence and antibiotic resistance profile of enterotoxin-producing S. aureus isolated from creamy pastries
Zahra Hashemi Mojtaba Bonyadian Hamdallah Moshtaghi

The present study aimed to determine the prevalence of enterotoxin-producing S.aureus in creamy pastries presented in confectionery in Shahrekord and to determine their antibiotic resistance. One hundred fifty samples were randomly selected from creamy pastries. Microbi More

The present study aimed to determine the prevalence of enterotoxin-producing S.aureus in creamy pastries presented in confectionery in Shahrekord and to determine their antibiotic resistance. One hundred fifty samples were randomly selected from creamy pastries. Microbial tests for isolation of S. aureus were performed by culturing in a nutrient broth and then cultured on a Beards Parker agar and suspected colonies were confirmed by standard complementary tests including gram staining, catalase test, and coagulase. Isolates were tested for presence of enterotoxin genes (A, B, C, D) by Multiplex PCR. Antibiotic resistance was determined by the disk diffusion method. Overall, 38 (25.3%) samples were contaminated with S. aureus. 21 isolates (28%) and 17 isolates (22.7%) were related to summer and winter samples respectively. Thirteen isolates (29%) of S. aureus contained enterotoxin genes. 11 isolates (84. 61%) contained SEA, and 2 isolates (5.3%) contained the SEC gene. Totally, 9 (70%) enterotoxigenic isolates were related to summer and 30% to winter samples. None of the isolates had SEB, SED genes. Four (30%) enterotoxigenic isolates related to creamy pastry and 9 (70%) to creamy bread. Antibiogram test showed that the highest sensitivity to sulfamethoxazole (97.36%) and erythromycin (63.15%). Also, all enterotoxigenic isolates were resistant to penicillin and 95% with SEA gene were resistant to erythromycin and ciprofloxacin.The results of present study revealed that contamination of creamy pastries with S. aureus capable of producing enterotoxin and resistance to some antibiotics. Manuscript profile

Open Access Article

9 - The antimicrobial effect of basil seed mucilage-ZnO nanocomposite coating on the quality of cheddar cheese during ripening
Sahar Kheirkhah Foghara sara jafarian Shahin Zomorodi Leila Roozbeh Asghar Khosrowshahi asl

Cheddar cheese is a hard type of cheese. It is coated traditionally by a special wax. Commercial coating materials which are used for coating are non-edible as a result they are undesirable due to environmental pollution and allergies caused in some consumers. Today, bi More

Cheddar cheese is a hard type of cheese. It is coated traditionally by a special wax. Commercial coating materials which are used for coating are non-edible as a result they are undesirable due to environmental pollution and allergies caused in some consumers. Today, biodegradable films are receiving more attention. In this study, the effect of edible coating of basil seed mucilage containing zinc oxide nanoparticles (ZnO-NP) in three levels of zero, 0.25 and 0.5% on the quality properties of cheddar cheese was investigated during ripening for 90 days. The results of experiments showed that at the end of ripening, the highest amount of salt and fat and the lowest amount of moisture were related to the sample with mucilage coating containing 0.5% of ZnO-NP. Also, during ripening, in all samples the number of non-starter lactic acid bacteria increased, but the number of starter bacteria increased first and then decreased (p < 0.01). Also, in all treatments, the total number of mesophilic aerobic bacteria, Pseudomonas, mold and yeast increased significantly during storage, which was the lowest in samples with coating containing ZnO-NP (p < 0.01). In addition, ZnO-NP coatings prevented mold growth on the surface of cheddar cheese samples. According to the results obtained of this study, basil seed mucilage containing 0.5% ZnO-NP can be used as a coating in cheddar cheese without any adverse effect on the flavor of cheese. Manuscript profile

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