List of Articles Hassan Momtaz

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  1 - Typing of the Acinetobacter baumannii strains isolated from row meat of poultry and livestock using Multi Locus Sequence Typing (MLST)
  Marziyeh Tavakol Hassan Momtaz Parviz Mohajeri Leili Shokoohizadeh Elahe Tajbakhsh
  Acinetobacter baumannii (A. baumannii) strains with multiple drug resistance are mainly opportunistic pathogens in the development of hospital infections and as an emerging contaminant in livestock-based foods. The aim of this study was to determine the antibiotic resis More

  Acinetobacter baumannii (A. baumannii) strains with multiple drug resistance are mainly opportunistic pathogens in the development of hospital infections and as an emerging contaminant in livestock-based foods. The aim of this study was to determine the antibiotic resistance pattern and genotyping of this bacterium strains in raw meat of poultry and livestock. 22 strains isolated from raw meat were tested by multi-locus sequence typing and simple disk diffusion methods. The highest antimicrobial resistance was observed to tetracycline with 90.9% and the least antibiotic resistance was azithromycin and imipenem with 9.09%. Five strains were identified as non-typing isolates in 22 isolates of A. baumannii. Five genetic profiles (Sequence Types=ST) including ST15, ST10, ST12, ST25, ST25 were identified. Identifying the acceptable level of genetic variation among isolates using the MLST technique indicates that this method is considered as a useful method in the study and typing of Acinetobacter spp. strains and can be strains isolated from different origins in different groups. In this study, it was found that by sequencing of house-keeping genes, it is possible to typing of Acinetobacter spp. strains, and this amount of polymorphism indicates that this technique is a useful method for analyzing the genetic diversity of A. baumannii strains is a source of animal origin. Manuscript profile
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  2 - Prevalence and phylogenetic analysis of Listeria monocytogenes isolated from the fillets of two farmed fish in Shahrekord in 2018
  Esmaeil Pirali Khairabadi Seyedh Sedigheh Mousavi Hassan Momtaz Farzaneh Nikokhah Seyed Pejman Hosseini Shekarabi Mehdi Raissy
  Fish and other aquatic animals, directly and indirectly, can be contaminated with Listeria monocytogenes and consequently, fish and their products are considered a carrier for this bacterium. The present study investigated the contamination of farmed rainbow t More

  Fish and other aquatic animals, directly and indirectly, can be contaminated with Listeria monocytogenes and consequently, fish and their products are considered a carrier for this bacterium. The present study investigated the contamination of farmed rainbow trout and common carp with this bacterium as well as the phylogenetics of the isolates. A total of 50 samples of farmed fish were obtained from local fish supply centers in Shahrekord City from July to September 2018. Based on the molecular experiments, 18 (36%) of the 50 studied samples were contaminated with L. monocytogenes as 26% and 10% of the isolates were found in trout and carp, respectively. From 18 isolates, 12 samples (66.66%) belonged to serotype 4b, one belonged to serotype 1/2a and the other 5 samples belonged to serotype 1/2b. According to the results of the present study and regarding the importance of the bacteria in terms of public health, continuous and accurate hygienic monitoring seems to be necessary. Manuscript profile
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  3 - The Study on antibiotic resistance pattern of Cronobacter sakazakii strains isolated from infant formula and baby food types
  Samira abbasi bafetrat Mohammad Goli Hasan Momtaz
  Cronobacter sakazakii is one of the main pathogens that transmitted by feeding infants by contaminated infant formula and baby-food. This bacterium is one of the major causes of mortality, digestive diseases and meningitis in newborns. This study was performed in order More

  Cronobacter sakazakii is one of the main pathogens that transmitted by feeding infants by contaminated infant formula and baby-food. This bacterium is one of the major causes of mortality, digestive diseases and meningitis in newborns. This study was performed in order to detect microbiological and molecular contamination by Cronobacter sakazakii and study antibiotic resistance pattern of bacterial isolates. Two hundred samples of infant formula and baby food from deferent brands were collected and after microbial detection, bacteria’s isolates were detected, using PCR method. Then antibiotic resistance pattern of isolates were evaluated using disk diffusion method. Three samples from total 100 baby food (3%) and 5 samples from total 100 infant formula (5%) had Cronobacter sakazaki infection. A, B and C brands from infant formula and A, D brands from baby food were contaminated with Cronobacter sakazakii. B and C brands from infant formula (10% each of them) and A brand from baby food (8%) were infected with Cronobacter sakazakii. Isolates from Cronobacter sakazakii which isolate from infant formula and baby food demonstrated most Sensitivity to ciprofloxacin (20.38), meropenem (19.83) and imipenem (19.63) and most resistance to amoxicillin, ampicillin, tigecycline, ticarcillin, aztreonam and ceftazidime. Careful inspection on the raw material quality of infant formula and baby food as well as, exact supervision on production procedure, sanitation in addition to use natural antimicrobial can reduce Prevalence rate of this bacteria. Manuscript profile
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  4 - SCCmec typing of Staphylococcus aureus strains isolated from milk and dairy products
  Narges Aminifard Hasan Momtaz Zahra Bamzadeh
  Staphylococcus aureus is a major opportunistic pathogen causing various diseases such as pneumonia and urinary tract infections. Indiscriminate and excessive use of antibiotics leads to antibiotic resistance in this bacterium, especially against commonly used antibiotic More

  Staphylococcus aureus is a major opportunistic pathogen causing various diseases such as pneumonia and urinary tract infections. Indiscriminate and excessive use of antibiotics leads to antibiotic resistance in this bacterium, especially against commonly used antibiotics in the treatment. The present study aimed to evaluate the antibiotic resistance pattern of Staphylococcus aureus strains isolated from milk and traditional milk products, especially resistance to methicillin. A total of 403 samples of milk and milk products was examined and collected from distribution centers of traditional and dairy products. Strains isolated were tested by PCR to determine the frequency of mec gene, SCCmec types and genes coding for antibiotic resistance. Antibiotic resistance pattern of isolates was assessed by disk diffusion method. Out of 403 samples, 151 samples (%44.67) were infected with Staphylococcus aureus, and the highest prevalence of infection was found in samples of cow raw milk (%58.55). Only IV type was detected in mecA-positive isolates among SCCmec types; a number of 66 isolates belonged to IVd type, 21 isolates related to IVc type and 13 isolates belonged to IVa type and the significant differences between SCCmec type IVd with two types IVc and IVa was observed. The high prevalence of infection with Staphylococcus aureus in raw milk and traditional milk products and high antibiotic resistance of the isolates to common types of antibiotics in treating human infections are serious warning to the community and it requires hygienic measures and quality control of dairy products more than ever. Manuscript profile
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  5 - Identification of Methicillin-Resistant Staphylococcus epidermidis (MRSE) Strains Associated with Food Poisoning Outbreaks in Clinical Samples
  Parisa Behshod Elahe Tajbakhsh Hassan Momtaz
  Methicillin-Resistant Staphylococcus epidermidis is an important pathogen that causes infectious diseases whose treatment is extremely formidable. Staphylococcus epidermidis enterotoxins with effects on intestinal epithelial cells can are be causing Create food poisonin More

  Methicillin-Resistant Staphylococcus epidermidis is an important pathogen that causes infectious diseases whose treatment is extremely formidable. Staphylococcus epidermidis enterotoxins with effects on intestinal epithelial cells can are be causing Create food poisoning in people. The aim of current study is to the identification of MRSE strains associated with food poisoning outbreaks in Isfahan. During six- months, 60 clinical specimens to isolated from strains of Staphylococcus epidermidis were screened. Following identification strains, MRSE isolates were isolated by PCR method and, and then antibiotic resistance pattern of them was determined by Kirby – Bauer method. The presence of the sea, seb, sed and, sei genes was analyzed by PCR. 45 isolates of Staphylococcus epidermidis were isolated from 60 samples, 30 isolate (66.6 percent) were MRSE. MRSE isolates exhibited the highest rates of resistance to penicillin (80 percent), and cefoxitin (56.6 percent), while they showed the lowest resistance to levofloxacin (13.3 percent), and rifampicin (6.6 percent). The prevalence rate of Moreover, the frequency of enterotoxin genes sea, seb, sed and, sei was 60 percent, 63.3 percent, 13.3 percent and, 76.6 percent respectively, in the isolate. In this study, high percentage of MRSE isolates were antibiotic resistant and produced enterotoxin. Considering that these toxins are superantigen and can more intense the complications of clinical and nosocomial infections, detecting and rapid treatment of these infections are essential. Manuscript profile
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  6 - Genotyping of Escherichia coli O157: H7 strains isolated from raw ruminant and poultry meat samples by RAPD-PCR
  Mandana Lotfi Hassan Momtaz Elahe Tajbakhsh
  Escherichia coli O157:H7 is one of the most important enteric pathogenic strains that is usually transmitted to humans through contaminated water and food. These strains cause hemorrhagic colitis, HUS syndrome, cytopenic purpura thrombosis, and, in some cases, death. Th More

  Escherichia coli O157:H7 is one of the most important enteric pathogenic strains that is usually transmitted to humans through contaminated water and food. These strains cause hemorrhagic colitis, HUS syndrome, cytopenic purpura thrombosis, and, in some cases, death. The main repository of these bacteria is ruminants. The aim of this study was genetic classification (genotyping) of isolates of this strain isolated from raw meat and poultry by RAPD-PCR method. 344 samples of ruminants and poultry were collected from fresh meat supply centers in Isfahan and Shahrekord. Genetic classification of O157:H7 isolates was performed by the RAPD-PCR method. Of 344 raw meat samples studied, 202 Escherichia coli isolates (58.7%) were isolated. The rate of O157: H7 strain was 17.8% (36 samples). Genetic classification of Escherichia coli O157:H7 isolates showed 9 different profiles among these 36 isolates and found an affinity of 43.7 to 100% among the isolates. This study showed high molecular similarity between E. coli O157: H7 strains isolated from different animals in Isfahan and Shahrekord. It was also found that RAPD-PCR is a simple, fast, and inexpensive method for describing the genetic diversity of different E. coli strains including strain O157:H7. Manuscript profile
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  7 - Occurrence and antibiotic resistance of Enterococcus faecalis strains isolated from fish and shrimp caught from the Persian Gulf
  Neda Noroozi Hassan Momtaz Elahe Tajbakhsh
  10.30495/jfm.2022.1938722.1730
  The present study was performed to evaluate the occurrence and pattern of antibiotic resistance of Enterococcus faecalis strains isolated from fish and shrimp samples caught from the Persian Gulf. A total of 240 seafood samples caught from the Persian Gulf, including 12 More

  The present study was performed to evaluate the occurrence and pattern of antibiotic resistance of Enterococcus faecalis strains isolated from fish and shrimp samples caught from the Persian Gulf. A total of 240 seafood samples caught from the Persian Gulf, including 120 fish and 120 shrimp samples were collected from Bushehr province. The presence of E. faecalis was confirmed using microbial culture and biochemical tests. The pattern of antibiotic resistance of E. faecalis isolates was evaluated using Disc Diffusion Method. Fifty-four out of 240 (22.50%) samples were contaminated with E. faecalis. The contamination of E. faecalis in fish and shrimp samples were 30% and 15 percent, respectively. E. faecalis strains isolated from fish and shrimp samples had the highest resistance to gentamicin (100%), tetracycline (100%), erythromycin (100%), cefazolin (90.74%) and trimethoprim-sulfamethoxazole (88.88%) antibiotics. Resistance to chloramphenicol was not observed in any of the isolates. The results of the study showed that fish and shrimp can be considered as possible sources of antibiotic-resistant E. faecalis. Therefore, complete cooking of seafood before consumption, observance of hygiene in fishing and sale centers and prescribing antibiotics according to the results of the disk diffusion test can prevent gastrointestinal infections caused by antibiotic-resistant strains of E. faecalis. Manuscript profile
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  8 - Detection and antibiotic resistance pattern of Listeria monocytogenes strains isolated from curd and cheese
  Rahil Kiyanpour Berjoee Hassan Momtaz Lida Lotfollahi zahra bamzaheh
  10.30495/jfm.2022.1943942.1741
  Listeria monocytogenes is a foodborne pathogen that causes listeriosis in humans and animals. Microbial contamination of food usually leads to widespread food poisoning in the form of widespread epidemics in the region, which is very significant in terms of public healt More

  Listeria monocytogenes is a foodborne pathogen that causes listeriosis in humans and animals. Microbial contamination of food usually leads to widespread food poisoning in the form of widespread epidemics in the region, which is very significant in terms of public health and is one of the most important issues in developing countries. The aim of this study was to determine the contamination of Listeria monocytogenes in dairy products using culture method and final confirmation by PCR method. In this study, 150 different dairy samples offered in the market were purchased randomly. The samples were transferred to the laboratory under hygienic conditions and examined. In addition to culture medium experiments, positive samples were evaluated for final confirmation and identification of the pathogen by molecular PCR.14 (9.33%) positive samples including 6 samples of white cheese (4%), 4 samples of cream cheese (2.6%), and 3 samples of curd (2%) were positive for contamination. Antimicrobial susceptibility to 16 antibiotics was highly sensitive to clindamycin (47.37%). It is noteworthy that it was resistant to several drugs.The presence of Listeria monocytogenes in dairy products (cheese, cream cheese, and whey) was proven. Based on the results, people who consume contaminated dairy products are at potential risk of listeriosis. As a result, food safety authorities must establish an effective standard for examining the presence of Listeria in food. Manuscript profile
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  9 - Comparison of antibiotic resistance and virulence factors in Enterococcus faecalis isolates isolated from animal and human sources
  Mahmoud Shahveh Elahe Tajbakhsh Hassan Momtaz Reza Ranjbar
  Enterococci are an important and diverse group of bacteria that are known to be resistant to most antibiotics used to treat diseases. In this cross-sectional study, 104 samples of red meat and 1000 urine samples suspected of urinary tract infection in the border city of More

  Enterococci are an important and diverse group of bacteria that are known to be resistant to most antibiotics used to treat diseases. In this cross-sectional study, 104 samples of red meat and 1000 urine samples suspected of urinary tract infection in the border city of Kermanshah were examined for Enterococcus faecalis. First, the samples were approved by biochemical and molecular methods, then in order to evaluate their ability to produce biofilm, Microtiter Plate method was used and their sensitivity to antibiotics was also determined by Kirby-Bayer method. Enterococcus faecalis infection in human samples and red meat samples was reported to be 5% and 40.38% respectively. In the strains isolated from red meat samples, the highest resistance was reported to be to Streptomycin while the lowest resistance was to Vancomycin. In the human isolate samples, the highest resistance was reported to be to Co-trimoxazole ,while the lowest resistance was to Nitrofurantoin . In strains isolated from red meat, ebp A, ebp B and ebp C were reported to be 71.43%, 59.52% and 64.28% respectively. No statistically significant relationship was observed between biofilm production and ebp genes in these isolates. However, in strains isolated from urine, a significant relationship was detected between ebp genes and biofilm production. Similarly, it was reported that there was no statistically significant relationship between the meat type and the virulence gene type. But, the findings of the study showed a significant relationship between the frequency of efa A, gel E, ace and esp genes. Manuscript profile
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  10 - Frequency of binding genes in Staphylococcus aureus isolated from dairy products in Shahrekord. Binding genes of Staphylococcus aureus in dairy products
  Rasul Pajohesh Elahe Tajbakhsh Hassan Momtaz Ebrahim Rahimi
  10.30495/jfm.2022.1971880.1795
  Staphylococcus aureus is an opportunistic pathogen in humans and animals, which can cause contaminating dairy products. In this research, 250 traditional dairy products, including butter, yogurt, cheese, curd, and cream, were prepared from different parts of Shahrekord More

  Staphylococcus aureus is an opportunistic pathogen in humans and animals, which can cause contaminating dairy products. In this research, 250 traditional dairy products, including butter, yogurt, cheese, curd, and cream, were prepared from different parts of Shahrekord city and transported to the microbiology laboratory of Shahrekord Azad University in sterile containers with ice. The isolation of Staphylococcus aureus bacteria was done according to standard protocols and molecular methods. Genetic evaluation of biofilm was done by PCR method to detect the most common biofilm coding genes (icaA, icaB, icaC, icaD, bap, fnbA, fnbB, clfB, clfA). From the total of 250 examined samples, 50 samples (20%) were positive for Staphylococcus aureus. Contamination was reported in 26% of curd, 24% of butter, 22% of cheese, 16% of cream, and 12% of yogurt. The frequency of the mentioned genes was reported as 74%, 68%, 64%, 76%, 30%, 96%, 90%, 32% and 28% respectively. In the microtiter plate method, biofilm reaction was observed in 30 isolates (60%), strong biofilm was observed in 20 isolates (66.66%), and weak biofilm was observed in 10 isolates (33.33%). No biofilm reaction was observed in 5 isolates (16.66%). Based on Fisher's exact test, a statistically significant relationship was reported between the studied genes and strong and moderate biofilm reaction. Manuscript profile
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  11 - Determining antibiotic resistance pattern in Enterobacter cloacae strains isolated from checken and sheep meat in Shahrekord city
  elahe barzam الهه تاج بخش Hassan Momtaz
  Usually, contaminated food is one of the main causes of human infections, and in this case, poultry meat and sheep are considered as the main causes. Enterobacter cloacae strains, having different virulence factors and multiple antibiotic resistance are mainly considere More

  Usually, contaminated food is one of the main causes of human infections, and in this case, poultry meat and sheep are considered as the main causes. Enterobacter cloacae strains, having different virulence factors and multiple antibiotic resistance are mainly considered as an opportunistic pathogen. In this research, the isolation of Enterobacter cloacae from chicken and sheep meat samples in Shahrekord in 2019 was done by microbial and molecular methods. Antibiotic resistance pattern was determined by disc diffusion method and microtitre plate method was used to check biofilm production. The ability to produce broad-spectrum β-lactamase enzymes was investigated through phenotypic and genotypic methods. Out of 384 examined samples, Enterobacter cloacae were identified in 25 samples (6.51%) which also confirmed in the presence of the hsp60 in molecular analysis. Among these, 18 samples were related to chicken meat (72%) and 7 samples (28%) were related to sheep meat. The highest antibiotic resistance to cotrimoxazole and cefotaxime was reported in 20 isolates (80%) and the lowest resistance to nitrofuranthein was reported in 15 isolates (23.8%). In microtiter method. 15 isolates (60%) showed strong biofilm reaction, 10 isolates (40%) showed moderate biofilm reaction. The present study indicates that ESBL-producing Enterobacter cloacae strains have a relatively high prevalence. The increase in the number of these strains is often caused by the irrational prescription of antibiotics, which requires the use of new antimicrobial agents,. Manuscript profile
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  12 - Application of ERIC-PCR method for genetic classification of campylobacter strains isolated from raw milk
  GholamReza Banisharif Mohammad Hosein Marhamatizadeh Hassan Momtaz
  Introduction and purpose: Campylobacter species are one of the most important pathogens causing bacterial gastroenteritis, which are generally transmitted through food of animal origin. The present study was conducted with the aim of genetic classification of Campylobac More

  Introduction and purpose: Campylobacter species are one of the most important pathogens causing bacterial gastroenteritis, which are generally transmitted through food of animal origin. The present study was conducted with the aim of genetic classification of Campylobacter isolated from raw milk of cows, sheep and goats in Chaharmahal and Bakhtiari province. Methods: 43 isolates of Campylobacter isolated from raw milk of cows, sheep and goats in Chaharmahal and Bakhtiari provinces were selected and confirmed by ERIC-PCR method. Results: The studied isolates revealed banding patterns ranging from 100 to 2000 open pairs, which were further classified into 5 main profiles with a similarity coefficient of simple matching at a similarity level above 80%. Except for 100% affinity which was observed in 1 case, other isolates had genetic affinity between 54% and 98%. Conclusion: The placement of the studied isolates in several subgroups showed the acceptable discrimination power of the ERIC-PCR technique in Campylobacter genotyping and the presence of different sources of contamination of dairy products with this pathogen. ERIC-PCR method is a simple, fast and low-cost method to describe the genetic diversity of different Campylobacter strains, including Campylobacter jejuni and coli strains. Manuscript profile