The Study on antibiotic resistance pattern of Cronobacter sakazakii strains isolated from infant formula and baby food types

Subject Areas : Food Microbial Contamination

Samira  abbasi bafetrat ¹ (M.S. Graduated of Food Science and Technology, Faculty of Agriculture, Isfahan(Khorasgan) Branch, Islamic Azad University, Isfahan, Iran)
Mohammad  Goli ² (Department of Food Science and Technology, Faculty of Agriculture, Isfahan(Khorasgan) Branch, Islamic Azad University, Isfahan, Iran)
Hasan  Momtaz ³ (Department of Microbiology, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran)

Received: 2016-06-14 Accepted : 2017-02-05 Published : 2017-02-19

Keywords: Cronobacter sakazakii, Baby food, Infant formula, Antibiotic resistance pattern,

Abstract :

Cronobacter sakazakii is one of the main pathogens that transmitted by feeding infants by contaminated infant formula and baby-food. This bacterium is one of the major causes of mortality, digestive diseases and meningitis in newborns. This study was performed in order to detect microbiological and molecular contamination by Cronobacter sakazakii and study antibiotic resistance pattern of bacterial isolates. Two hundred samples of infant formula and baby food from deferent brands were collected and after microbial detection, bacteria’s isolates were detected, using PCR method. Then antibiotic resistance pattern of isolates were evaluated using disk diffusion method. Three samples from total 100 baby food (3%) and 5 samples from total 100 infant formula (5%) had Cronobacter sakazaki infection. A, B and C brands from infant formula and A, D brands from baby food were contaminated with Cronobacter sakazakii. B and C brands from infant formula (10% each of them) and A brand from baby food (8%) were infected with Cronobacter sakazakii. Isolates from Cronobacter sakazakii which isolate from infant formula and baby food demonstrated most Sensitivity to ciprofloxacin (20.38), meropenem (19.83) and imipenem (19.63) and most resistance to amoxicillin, ampicillin, tigecycline, ticarcillin, aztreonam and ceftazidime. Careful inspection on the raw material quality of infant formula and baby food as well as, exact supervision on production procedure, sanitation in addition to use natural antimicrobial can reduce Prevalence rate of this bacteria.