Optimization of the Extraction Process of Aqueous and Alcoholic (Ethanol) Extracts of Allium Ampelloprasuml Plant by Response Surface Methodology and Its Antioxidant Effect on Soybean Oil Stability over Shelf Life
Subject Areas : Food Microbial Contaminationالهام Azadfar 1 , Maryam Sabetghadam 2 , زهره Bahrami 3 , بیتا Beyzaei 4
1 - Ph.D. student in Food Science and Engineering, Young and Elite Researchers Club, Sabzevar
Branch, Islamic Azad University, Sabzevar, Iran
2 - Ph.D. student in Food Science and Engineering, Young and Elite Researchers Club, Sabzevar
Branch, Islamic Azad University, Sabzevar, Iran
3 - Expert in Food Industry, Sabzevar Branch, Islamic Azad University, Sabzevar, Iran
4 - Expert in Food Industry, Sabzevar Branch, Islamic Azad University, Sabzevar, Iran
Keywords: Response Surface, Peroxide, Allium ampelloprasumL, Radical scavenging power,
Abstract :
Plants contain phenolic compounds ranging from simple molecules such as phenolic acids to highly polymerized molecules such as tannins Phenols and polyphenols in plant tissues have gained increasing scientific interest because of their potential beneficial effects on human health..In this study, Allium ampelloprasum L plant was prepared and homogenized from Sabzevar. The extraction was performed by the ultrasound extraction method using ethanol and water solvent. The amount of and antioxidant compounds of extracts was investigated by and DPPH methods, respectively. The results of the optimization process have shown that the optimum conditions of alcoholic extraction were determined as 17.81 minutes with the concentration of 800. Also, the optimum conditions for the aqueous extraction were determined as the concentration of 800 ppm and the time of 13.52 minutes. According to the optimization results, the extraction amount of antioxidant compounds in aqueous and alcoholic extractions were reported as 33.893-33.499, respectively. The results of the oxidative stability of oil have shown that the peroxide and thiobarbituric acid index in water and alcoholic extraction were 1.938-3.158 and 0.173-0.337, respectively.
اسماعیل زاده کناری، ر.، و مهدی پور، س. (1391). اثرآنتی اکسیدانی عصاره متانولی پوست کیوی درپایداری سازی روغن آفتاب گردان. پژوهشهای علوم و صنایع غذایی ایران، جلد8، شماره 2، صفحه250-245.
افرازه، ز.، بلندی، م.، خورشیدی، م.، و محمدی نافچی، ع. (1393). بررسی فعالیت آنتی اکسیدانی عصارههای آبی و الکلی (متانول، اتانول) گلبرگ زعفران، زراعت و فناوری زعفران، جلد2، شماره 3، صفحه 236-231.
فیاض مهر، ب.، و آصفی، ن. (1391). تأثیر امواج فراصوت بر مقدار و ظرفیت آنتی اکسیدانی لیکوپن استخراج شده از تفاله گوجه فرنگی. پژوهشهای صنایع غذایی، جلد22، شماره2، صفحه 248-242.
Abootalebian. M. Keramat. J. Kadivar. M. Ahmadi. F. and Abdinian. M. 2016. Comparison of total phenolic and antioxidant activity of different Mentha spicata and M. longifolia accessions. Annals of Agricultural Sciences, 61 (2), 175-179
Agregán. R. Lorenzo. J. M. Munekata. P. E. Dominguez. R. Carballo. J. and Franco. D. 2016. Assessment of the antioxidant activity of Bifurcaria bifurcata aqueous extract on canola oil. Effect of extract concentration on the oxidation stability and volatile compound generation during oil storage. Food Research International. In press
Bagheri. AR. 2006. Fundamentals of Plant Tissue Culture (eds. R. L. Piric). Mashhad: Ferdosi University of Mashhad.
Ben Salem. M. Affes. H. Athmouni. K. Ksouda. K. Dhouibi. R. Sahnoun. Z. Hami, S. and Zeghal. K. M. 2017. Chemicals Compositions, Antioxidant and Anti-Inflammatory Activity of Cynara scolymus Leaves Extracts, and Analysis of Major Bioactive Polyphenols by HPLC. Evidence-Based Complementary and Alternative Medicine.
Burits. M. Bucar. F. 2000. Antioxidant activity of Nigella sativa essential oil. Phytotheraphy Research 14, 323–328.
Chirinos. R. Rogez. H. Campos. D. Pedreschi. R. and Larondelle. Y. 2007. Optimization of extractionconditions of antioxidant phenolic compounds from mashua (Tropaeolum tuberosum Ruız & Pavon) tubers. Separation and Purification Technology 55: 217–2
David Raja. H. and Arockiasamy. DI. 2008. In vitro Propagation of Mentha viridis L. from Nodal and Shoot tip Explants. Plant Tissue Cult & Biotech, 18:1-6.
14-Farhoosh. R. and Moosavi. S.M.R. 2006. Determination of carbonylvalue in rancid oils: a critical reconsideration. J Food Lipids. 13: 298–305.
Ferreres. F. Sousa. C. Valento. P. Seabra. R. M., Pereira. J. A. and Andrade. P. B. 2007. Tronchuda cabbage (Brassica oleracea L. var. costata DC) seeds: phytochemicalcharacterization and antioxidant potential. J Food Chem., 101: 549-558
Ghafoor. K. and Choli, H. 2009. Optimization of ultrasound assisted extraction of phenolic compound and antioxidant fram grape peel through response surface methodology. J Bilogical chemistry 52 (3):295-300.
Gülçın. İ. Oktay. M. Kıreçcı. E. Küfrevıoǧlu. Öİ. 2003. Screening of antioxidant and antimicrobial activities of anise. (Pimpinella anisum L.) seed extracts. Food chem; 83 (3): 371-82.
Hismath. Wan Aida. 2011. Optimization of extraction conditions for phenolic compounds from neem (Azadirachta indica) leaves. J International Food, 18 (3):931-939.
Horwitz. W. 1975. Official methods ofanalysis of the association of official analytical chemist. (AOAC). 13th ed.
Morella. L. and Prado. M.A. 2012. Extraction optimization for antioxidant phenolic compounds in red grape jam using ultrasound with a response surface methodology. Ultrasonics Sonochemistry, 19:1144-1149.
Muret. K. Sevgi. K. Sengul. K. Esra. U. Cemalettin. B. Fedra. V. 2007. Biological activities, chemical composition of three honeys of different types from Anatolia. Food Chem. 100 (2): 526-534.
Negi. P.S. 2012. Review Plant extracts for the control of bacterial growth: Efficacy, stability and safety issues for food application. Int J Food Microbiol; 156: 7-17.
Olugbami. J. O. Gbadegesin. M. A. and Odunola. O. A. 2015. In vitro free radical scavenging and antioxidant properties of ethanol extract of Terminalia glaucescens. Pharmacognosy research, 7 (1), 49-55.
Rostagno. A. Palma. M. and Barroso, C. 2003. Ultrasound assisted extraction of soy isoflavones. J Chromatography A, 1012: 119-128.
Seabury. k. 2002.The effect of antioxidants in preventing farther oxidation in TBA analysis. California state science fair. Project number, Jo404.
Stoilova. I. Krastanov. A. Stoyanova. A. Denev. P. and Gargova. S. 2007. Antioxidant actirity of ginger extract (Zingiber officinale). Food Chem; 102: 764-70.
Suzuki. M. Watanabe. T. Miura. A. Harashima. E. Nakagawa. Y. and Tsuji. K. 2002. An extractionsolvent optimum for analyzing polyphenol contents by Folin-Denis assay. Nippon Shokuhin Kagaku Kaishi 49: 507-511.
Yang. Y. Song. X. Sui. X. Qi, B. Wang. Z. Li, Y. and Jiang. L. 2016. Rosemary extract can be used as a synthetic antioxidant to improve vegetable oil oxidative stability. Industrial Crops and Products, 80, 141-147.
Yassari. S. and Yasari. E. 2013. Effects of extracts of Thompson orange peel on the stability of canola oil. J International of Agriculture and Crop Sciences. 5-5:410-420
Zargari. A. 1996. Medicinal Plants. Tehran: Tehran University Publications: 437.
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