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      • Open Access Article

        1 - Evaluation of Escherichia coli population in intestine and meat of broilers by cinnamon and thyme supplement in chicken diet
        Ahmad Zeinodini Majid Gholami-Ahangaran Ebrahim Rahimi
        For evaluation and comparing effect of thyme and cinnamon on carcass contamination, 135 broiler chicks divided to 3 groups by 3 replicates. All chickens reared under same condition and received 0.5 percent thyme and 0.5 percent cinnamon on basic diet, continuously. Cont More
        For evaluation and comparing effect of thyme and cinnamon on carcass contamination, 135 broiler chicks divided to 3 groups by 3 replicates. All chickens reared under same condition and received 0.5 percent thyme and 0.5 percent cinnamon on basic diet, continuously. Control chickens received no supplement in basic diet. In 42 days old, chickens were slaughtered and breast muscle was sampled for Escherichia coli (E.coli) detection. Cecal content were sampled for E.Coli colony counting, by conventional bacteriologic methods. Results showed that administration of 0.5 percent thyme can reduce carcass contamination and E.coli population in cecal content, significantly. There was no significant difference between control chickens and chickens received cinnamon for percentage of E.coli detection on carcass and cecal Ecoli population. all studied indices. According to results of this study, continuous consumption of 0.5 percent of thyme can improve hygienic quality in broiler chickens while this effect not expected for cinnamon. Manuscript profile
      • Open Access Article

        2 - The study of Escherichia coli contamination rate in meats of chickens without antibiotic and conventional
        saeed Dehghani Majid Gholami-Ahangaran Ebrahim Rahimi
        The residue of antibiotics and other chemical compounds in chicken meat is one of main concerns in consumers of this protein source. In this research, for study of correlation between antibiotic consumption and chicken meat contamination, in summer of 2014, 10 chicken f More
        The residue of antibiotics and other chemical compounds in chicken meat is one of main concerns in consumers of this protein source. In this research, for study of correlation between antibiotic consumption and chicken meat contamination, in summer of 2014, 10 chicken farms in Isfahan province that reared under conventional condition and 5 chicken farms that reared broilers under without antibiotic consumption were monitored from start of growing period. At end of growing period, in slaughter stage, one piece of breast muscle and cecum content were sampled. The percentage of carcass contamination and number of Escherichia coli (E.coli) in cecal content were determined in chickens reared under conventional and without antibiotic condition. Also, the shigatoxogenic E.coli was examined by PCR.Furthermore, the carcass contamination and E.coli colony count in cecal content in chickens with or without colibacillosis background were determined. The results showed that the chicken meat contamination to E.coli and the E.coli population in cecal content in chickens reared under conventional condition were not significant different from chickens reared without antibiotic, while these indices in chickens with colibacillosis history were significant higher than chickens without colibacillosis history. In this study the shigatoxogenic E.coli was not detected. In overall, this study revealed microbial infection in chicken farms that lead to antibiotic consumption could decrease hygienic quality of chicken meat. Therefore, in addition to controlling of withdrawal time for antibiotic consumption, the withdrawal time for infections incidence must be observed for decrease the risk of transmission of pathogens along the food chain of human. Manuscript profile
      • Open Access Article

        3 - The comparing of antibiotic resistance pattern in Escherichia coli isolates from chicken meat that reared under conventional and without antibiotic condition
        Mohammadreza Shahiri Majid Gholami-Ahangaran Ebrahim Rahimi
        The using of antibiotics in chicken production is one of main concerns in consumers of this protein source that usually these compounds administrated for antimicrobial effect and control of microbial infections in poultry production. For comparing the antibiotic resista More
        The using of antibiotics in chicken production is one of main concerns in consumers of this protein source that usually these compounds administrated for antimicrobial effect and control of microbial infections in poultry production. For comparing the antibiotic resistance pattern in Escherichia coli (E. coli) that isolated from different growing conditions (conventional and without antibiotic), the E. coli strains were isolated from chicken meat and cecal content of chickens that were reared under conventional and without antibiotic conditions, in Isfahan province. After purification of E.coli strains on specific bacterial culture, and approve of bacterial strains according to biochemical tests, the isolates were cultured on Mueller-Hinton culture. The antibiotic resistance pattern was examined by disc-diffusion agar test. The results showed that the antibiotic resistance to different medicinal and veterinary antibiotics groups has occurred in two different growing conditions but the antibiotic resistance was lower in without antibiotic than conventional growing condition. Manuscript profile
      • Open Access Article

        4 - Comparison of Nanosil and hydrogen peroxide in reducing of Escherchia coli population on egg shell surface
        Majid Gholami-Ahangaran Mohsen Jafarian-Dehkordi Mazyar Haj-salehi
        Abstract In this study the efficiency of two commercial anti-bacterial compounds comprising hydrogen peroxide and Nanosil D2 for reducing of bacterial population on egg shell surface was compared. Thus, 90 eggs were prepared and divided into 3 groups. In first group, Na More
        Abstract In this study the efficiency of two commercial anti-bacterial compounds comprising hydrogen peroxide and Nanosil D2 for reducing of bacterial population on egg shell surface was compared. Thus, 90 eggs were prepared and divided into 3 groups. In first group, Nanosil, in second group, hydrogen peroxide and in third group sterile water (as control) were sprayed on outer surface of egg shell. After 30 minutes, crushed egg shells were cultured. After preparation of serial dilution, Escherichia coli colonies were identified and counted according to usual bacteriological procedures. The results revealed there are no significant differences in amount of E. coli colonies between eggs exposed to Nanosil and hydrogen peroxide. The eggs exposed to Nanosil and hydrogen peroxide showed significant lower infectivity comparing to the control group. Thus, it seems that Nanosil can be used as a disinfectant of eggs. Manuscript profile