Investigating the antioxidant properties of grape seed extracts of Iranian varieties and evaluating the antimicrobial effect on pathogenic pathogens isolated from poultry
Subject Areas : Food Microbial Contamination
Sayed Ahmad Nourbakhsh
1
,
Ebrahim Rahimi
2
1 - Department of Food Hygiene, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2 - Department of Food Hygiene, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Keywords: انگور, آنتیاکسیدان, کروماتوگرافی مایع با کارایی بالا, ویژگی ضدمیکروبی,
Abstract :
The aim of the present study is to investigate the antioxidant and antimicrobial properties of hydro alcoholic and aqueous extracts of grape seed varieties on Gram-positive and Gram-negative microorganisms isolated from poultry meat. 94 poultry meat samples including chicken, quail, turkey and ostrich were randomly collected from sales centers of Isfahan province. Microorganisms were identified by biochemical tests. Aqueous and hydro alcoholic extracts of grape seeds of black alfi, black gohari, black shahani, Rish-Baba, Shasat-arus and Rooster tail varieties were prepared by soaking method. Antimicrobial properties of hydro alcoholic and aqueous extracts of grape seeds were investigated by disk diffusion and MIC (minimum inhibitory concentration) methods. The antioxidant property of the extracts was evaluated by DPPH method and the IC50 value was calculated. The phenolic compounds present in aqueous and hydro alcoholic extracts of grape seeds were measured by high-performance liquid chromatography. The frequency of Arcobacter butzleri, Campylobacter jejuni, Listeria monocytogenes and Staphylococcus aureus was 11.45%, 17.70%, 1.04% and 16.66%, respectively. The antimicrobial effect of aqueous and alcoholic extracts of grape seed was higher on gram positive bacteria in both methods. Alcoholic extracts had more antimicrobial effect than aqueous extracts, Rish-Baba grape showed the most antimicrobial effect. The highest percentage of DPPH in hydro alcoholic extracts is related to black alfi and Rish-Baba grape seed extracts, and in aqueous extracts of black alfi and black shahani grape seeds.
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