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Open Access Article
1 - Antibiotic resistance in patients with diabetic foot ulcers
Shima ShantiaeeDiabetes mellitus is a growing problem in today's modern societies. It is difficult to estimate the total number of people suffering from the disease. Approximately 20% of diabetic patients develop wound infections during their life Which in the absence of effective tre MoreDiabetes mellitus is a growing problem in today's modern societies. It is difficult to estimate the total number of people suffering from the disease. Approximately 20% of diabetic patients develop wound infections during their life Which in the absence of effective treatment can disrupt the quality of life of these people. On the other hand, treatment of this complication is very costly. DFIs diabetic foot infections are one of the most important public health issues and the identification of microorganisms that cause microbial infections An antibiotic is good for finding an appropriate treatment. Meanwhile, many reports have shown that antibiotic resistance is rising dramatically. Therefore, early diagnosis of lesions and the rapid onset of antimicrobial treatment are essential for controlling infection and preventing complications and improving the quality of life. An antibiotic susceptibility test is needed to manage infection, which can help in choosing the best treatment options. Manuscript profile -
Open Access Article
2 - مقایسه اثر کاپتوپریل و اومپرازول بر زخم دئودنوم ناشی از سیستئامین در موش صحرایی
فیروزه سقایی مرتضی ثمینی ایرج کریمی عابد کوهپایه -
Open Access Article
3 - بررسی زخم معده در اسب عرب به روش آندوسکوپی
دکترعلی حسن پور دکتر محمد قلی نادعلیان دکتر محمد رضا مخبر دزفولی -
Open Access Article
4 - Evaluation of antifungal activity of topical Rosmarinus efficinalis essential oil on infected cutaneous wound with Candida albicans in rats: Histopathological study
Nejati, H., Farahpour, M.R.*, Neiriz Naghadehi, M. .Today, due to side effects and development of drug resistance to Azol drugs and its derivatives,which used for treatment of Candida infections in either topical or oral forms, led to the use ofbiological materials. One of medicinal herb have been used more frequently, t MoreToday, due to side effects and development of drug resistance to Azol drugs and its derivatives,which used for treatment of Candida infections in either topical or oral forms, led to the use ofbiological materials. One of medicinal herb have been used more frequently, the past to thepresent, is Rosemary (Rosmarinus officinalis L.). In this study on 45 male Wistar-albino rats(weight 210±10 g), after general anesthesia, and a wound square with dimensions 1/5 in the 1/5cm area between the shoulder, immediately was applied to the wound 0.1 ml of the suspensioncontaining 1/5×107 CFU Candida albicans yeast. Then tested in three groups of 15 rats each(control, topical ointment containing 1.5% and 3% Rosmarinus officinalis oil) were randomlydistributed into 5 subgroups of 3 rats each (sample groups on different days) groups. End ofdays 4th, 8th, 12th, 16th and 20th from wounds of different groups, in order to histopathology andyeast counts by a special punch biopsy specimen. Investigation showed that the use of topical1.5 and 3 percent of Rosmarinus essential oil compared with the control group cause thedecreased substantially in infection rates and increased volume production of collagen and islined. According to the finding results, wound healing is better at 3% Rosmarinus officinalisessential oil ointment treated group, compared to lower treatment dose and control groups. Manuscript profile -
Open Access Article
5 - Histopathological evaluation of the effect of Mentha piperita essential oil on cutaneous wound healing in rats infected with C. albicans
N. Farhangi Ghaleh Joughi, M.R. Farahpour, M. Neiriz Naghadeh,Wound infections caused by Candida albicans has grown substantially in recent years.Expensive medications, side effects, and particularly, the development of drug resistance, led tothe use of biological materials may be considered as an alternative solution. Studies sho MoreWound infections caused by Candida albicans has grown substantially in recent years.Expensive medications, side effects, and particularly, the development of drug resistance, led tothe use of biological materials may be considered as an alternative solution. Studies show thatMentha piperita essential oil contains flavonoids and menthol and hence it has antibacterialproperties. We used 100 male Wistar rats (weight 195-205 g). One square surgical wound withdimensions of 1.5×1.5 cm were performed on the back of each animal and immediately becameinfected whit 0.1 ml of 1.5×107 CFU Candida albicans suspension. Then the rats were dividedinto 4 groups (control, placebo, treatments 1.5% and 3%) each with 25 rats and randomlydistributed into 5 subgroups each whit 5 rats (sample groups on different days). Wound healingactivity was performed by histological studies and yeast counts at the end of 4th, 8th, 12th, 16thand 20th days after surgery. According to the pathological findings, Mentha piperita essential oiltopically, significantly reduces inflammation and migration of leukocytes, and also significantlyincrease in vascular regeneration, epithelialization and migration of fibroblasts, comparedcontrol group. Mentha piperita essential oil, especially in higher doses (3%), increasedpathological factors affecting wound healing in rats was infected with Candida albicans. Manuscript profile -
Open Access Article
6 - Comparative study on geometric and histopathologic effects of polar, semi polar and non-polar fractions Artemisia absinthium extract in rat
Rezaei, A., Mohajeri, D., Ahmadizadeh, Ch., Jalilzadeh, M. .Recovery of Scars is a treatment challenge in some diseases and chronic disorders. For this reason, new compounds are used for rapid recovery of Scars and conglutination. It is expected that Artemisia absinthium as a galenical and herbal drug, has rapid recovery effects MoreRecovery of Scars is a treatment challenge in some diseases and chronic disorders. For this reason, new compounds are used for rapid recovery of Scars and conglutination. It is expected that Artemisia absinthium as a galenical and herbal drug, has rapid recovery effects in scars and sores because has anti- inflammation effects, activation of fibroblast cells and also antihyaluronidase effects cause the rapid recovery of sores. In this study, effects of Artemisia absinthium on recovery of sores as polar, semi polar and non-polar extractions of it are investigated on the rat as a second recovery. After anesthesia, with use of biopsy punch, Created circular sores with full thickness on the 70 female rats and recovery process were investigated in 5 groups. Drug administration and sores measures performed with analyzing of digital Scans, once a day for 21 days. For microscopic observations, gathered Samplings form this tissue in the 0, 3, 7, 14, 21 days, and microscopic symbols are ranged as edema factors and swelling reactions, hyperemia and bleeding, fibroblast, of Coverage tissue, torsion of sores and maturation of granular tissue. After histopathology and Calculation of recovery of sores scale for each drug, the finding results analyzed with SPSS software 17 versions. On the basis of geometric findings of recovery period, observed that semi polar extract of Artemisia absinthium has maximum Contraction of sores and control group has the least contraction of sores. Also, based on the histopathology results, total recovery in this group is better than other groups. In the second and third week. Recovered tissue has better organization than the other groups. Manuscript profile -
Open Access Article
7 - Study of effect of olive oil on re-epithelialization of epithelial tissue in excision wound healing model in rats
Abooei Mehrizi, M., Eidi A., Mortazavi, P. .Olive oil has been used for medical purposes. It has beneficial effects on cardiovascular and metabolic diseases, inflammatory and autoimmune diseases. The objective of this study was to find out the efficacy of topical administration of olive oil on dermal wound healin MoreOlive oil has been used for medical purposes. It has beneficial effects on cardiovascular and metabolic diseases, inflammatory and autoimmune diseases. The objective of this study was to find out the efficacy of topical administration of olive oil on dermal wound healing in rats. After creating full-thickness skin wounds on the back of 60 male Wistar rats, rats randomly divided into six groups, as control, sham, treatment and standard. Control group was intact. Sham group received eucerin as vehicle. Treated group received the olive oil at doses 2, 5 and 10%, dissolved in eucerin. Standard group were treated with phenytoin ointment 1%. Wound healing rats were calculated on days 3, 7, 11, 14, 18 and 21 post-wounding and the wound tissues were harvested at 3, 7, 14 and 21 days for histological studies. All of rats were sacrificed, and the skin around incision area was dissected and stained by Masson’s trichrome methods for tissue analysis. The results indicated a significant decrease in the diameter of wound in the treated and standard groups comparing to the control groups. A significant increase in the rate of re-epithelialization was observed at 14 days in the treated and standard groups compared to the control group. The results demonstrate that olive oil significantly accelerates cutaneous wound healing and its effect may be due to the increased re-epithelialization. It was concluded that it can be considered as a therapeutic agent for wound healing Manuscript profile -
Open Access Article
8 - Effect Fenugreek seeds hydroethanolic extract on a full-thickness wound healing in Streptozotocin-induced diabetic mice
Farahpour, M.R., Aghaei, M. .Disturbance in wound healing process is considered to be one of the most serious complications of diabetes, because it significantly increases the susceptibility of patient wounds to be infected. Fenugreek is an annual plant which its leaves and seeds used extensively i MoreDisturbance in wound healing process is considered to be one of the most serious complications of diabetes, because it significantly increases the susceptibility of patient wounds to be infected. Fenugreek is an annual plant which its leaves and seeds used extensively in foods and has advantages to human health. Due to prevalence rate and side effects of diabetes, Fenugreek seed extract is one of medicinal herb have been used more frequently. The present study was performed to investigate the effects of using fenugreek seed hydroethanolic extract on wound healing process in the 54 mice model of diabetes type 2. One single full-thickness excisional wound was created with 6-mm punch biopsy on back of each mice. All animal, accidentally, divided in three groups: Control group, 10% and 5% fenugreek seed hydroethanolic extract treatment groups. In terms of microscopic, the rate of tissue edema, neo-vascularization, fibroblast infiltration, collagen deposition and re- epithelialization were evaluated. Results revealed that the edema score significantly decreased (P<0.05) in both treatment groups compared to the control group, whereas the neo-vascularization, fibroblast infiltration, collagen deposition and re- epithelialization significantly increased (P<0.05) in both treatment groups compared to the control group. These results showed that topical administration of fenugreek seed hydroethanolic extract promote skin full-thickness excision wound healing process in diabetic animal model. Manuscript profile -
Open Access Article
9 - Study of the effect of supplement of soy isoflavone on the healing process of cutaneous wound in ovariectomized cats
, M Gholami , HR Moslemi , A Javaheri Vayeghan رضا Narenji Sani,Sterilization surgery in dogs and cats is a common surgical procedure in veterinary medicine, which can be done in two ways: Ovariohysterectomy (OHE) and Ovariectomy (OVE). Since some diets in cats contain lots of Genistein and Daidzein, studying of the effect of soy is MoreSterilization surgery in dogs and cats is a common surgical procedure in veterinary medicine, which can be done in two ways: Ovariohysterectomy (OHE) and Ovariectomy (OVE). Since some diets in cats contain lots of Genistein and Daidzein, studying of the effect of soy isoflavone on the wound healing process is essential. In this study, 10 cats of the DSH, 1 year old, were used. OVE was done by midline approach. After surgery, cats divided into two groups including isoflavone and placebo. The isoflavone group received 50 mg of oral isoflavone daily. In the control group, placebo was used. After 14 and 28 days, biopsy of the surgical wound was taken for histopathological examination to evaluate the amount of collagen, angiogenesis and fibroblasts. Based on the findings, On Day 14 after injury, wound micrographs showed that there was no significant difference between treatment and control groups (p < /em> ≥ 0.05). Furthermore, on day 28 after the injury, the healing rate in the treatment group was higher than control group significantly (P≤0.05). In summary, results of this study showed that the application of supplement of soy isoflavone could improve the healing process of cutaneous wound in ovariectomized cats. Manuscript profile -
Open Access Article
10 - Investigating the effect of Scenedesmus acuminates algae extract on healing wounds infected with Staphylococcus aureus in a rat model
هومن Akasheh, علیرضا Jahandideh امیر اقبال Khajerahimi شاپور Kakoolaki سعید HesarakiThe purpose of this study is to use a seaweed called Scenedesmus acuminates to reduce infection and heal wounds. Sixty rats were divided into four treatment groups, and sampling was done on the third, seventh, and fourteenth days after wound formation. No treatment was MoreThe purpose of this study is to use a seaweed called Scenedesmus acuminates to reduce infection and heal wounds. Sixty rats were divided into four treatment groups, and sampling was done on the third, seventh, and fourteenth days after wound formation. No treatment was done on the first group, the control group. The second group was treated with 25% zinc oxide as the drug of choice. The third and fourth groups were treated with 3% and 7% Scenedesmus acuminates seaweed ointment. The back wounds of mice were infected with 50 microliters of 2x108 CFU/mL Staphylococcus containing suspension. Histopathology and immunohistochemistry (CD68) were performed to compare the number of macrophages on tissue samples. On the third day after wounding, macrophages (CD68) were present in the algae group by 7% more than in the other groups. On the seventh day, fibroplasia and collagen synthesis in the algae group was 7% better than in the other two groups. On the 14th day, the accumulation of new blood vessels in the algae was 7% more than the others. The best result regarding the CD68 (macrophage) molecule expression level was seen in the 7% algae group on the 7th and 14th days. On the third, seventh, and fourteenth days, when macrophages are helpful, algae groups were better than others. The use of algae ointment can reduce the bacterial load of the wound and is better than the administration of zinc oxide. Manuscript profile -
Open Access Article
11 - Investigating the regenerative effects of PRP and polycaprolactone-hydroxyapatite zeolite nanocomposites on wound healing after tooth extraction
علیرضا Sagart علیرضا Jahandideh احمد Asghari ابوالفضل Akbarzadeh پژمان MortazaviWound healing after a tooth extraction is a complex process that helps repair wounds. Wound dressings are helpful for better healing. These agents should have the least inflammatory stimulation and the most antimicrobial properties. This study tried to investigate the e MoreWound healing after a tooth extraction is a complex process that helps repair wounds. Wound dressings are helpful for better healing. These agents should have the least inflammatory stimulation and the most antimicrobial properties. This study tried to investigate the effect of polycaprolactone-hydroxyapatite zeolite nanocomposites on the healing processes of the mucosa covering the alveoli after extraction. Fifteen healthy cats with lower premolar teeth caries were determined, and tooth extraction was performed. A biopsy sample was taken from the mucosa of the tooth extraction site on the 10th day after surgery. Premolar teeth of all 15 cats were extracted, and three groups were grouped as control, PRP, and nano polycaprolactone-hydroxyapatite zeolite. Histopathological analysis showed that unlike the control group and PRP, the nano group had a much better trend compared to the control group and the PRP group, with less presence of inflammatory cells, complete irregular epithelial tissue, more fleshy bud tissue, and more fibrosis. Polycaprolactone-hydroxyapatite zeolite nanocomposite accelerates wound healing after tooth extraction. Manuscript profile -
Open Access Article
12 - Protective effects of phoenixin 14 from the pathological point of view in experimental duodenal ulcer induced by indomethacin in rats
یاسر Zandeh‑Rahimi نگار Panahi سعید Hesaraki S.H Shirazi‑BeheshtihaPhoenixin-14 (PNX-14) is a newly identified neuropeptide with potential anti-inflammatory effects in the gastrointestinal tract. This study evaluated the protective effect of PNX-14 against the formation of duodenal ulcers caused by experimental indomethacin (IND). MorePhoenixin-14 (PNX-14) is a newly identified neuropeptide with potential anti-inflammatory effects in the gastrointestinal tract. This study evaluated the protective effect of PNX-14 against the formation of duodenal ulcers caused by experimental indomethacin (IND). Thirty-two male Sprague-Dawley rats were randomly assigned to four groups. They include negative control, IND (7.5 mg/kg subcutaneous indomethacin), FAM (7.5 mg/kg subcutaneous indomethacin followed by 40 mg /kg famotidine intraperitoneal), and PNX-14 (7.5 mg/kg subcutaneous indomethacin followed by 50 μg/kg intraperitoneal PNX-14). Outcome measures included macroscopic assessment of duodenal lesions and histopathological parameters. Selected parts of the duodenum were removed and placed in 10% formalin, and hematoxylin and eosin staining were prepared. The macroscopic grade of duodenal lesions in the PNX-14 group was significantly smaller than the IND group (P< 0.001). The components of tissue pathology lesions were significantly increased in the IND group. The wound's diameter and depth and the inflammation intensity in the PNX-14 group were significantly smaller than in the IND group (P<0.001). PNX-14 was superior to the FAM group in reducing inflammation. PNX-14 showed significant protective effects against IND-induced duodenal ulcer formation. These results show a promising therapeutic outcome for PNX-14 in treating inflammatory disorders of the gastrointestinal tract. Manuscript profile -
Open Access Article
13 - بررسی هیستولوژیک اثرات گیاه بابا آدم بر التیام زخم های جلدی در رت
رضا صداقت فرج الله ادیب هاشمی محمدرضا محمدی ملایری علیرضا مهاجرانی سید امین لطفی -
Open Access Article
14 - مقایسه اثر موضعی اسانس دو گیاه نعناع فلفلی (Mentha piperita) و رزماری (Rosmarinus officinalis) بر زخم عفونی شده با کاندیدا آلبیکنس (Candida albicans)در موش صحرایی
محمد رضا فرهپور احمد اصغری نازیلا فرهنگی قلعه جوقی حسین نجانی -
Open Access Article
15 - Effect of hydroalchoholic extract of Fraxinus extract in incisional wound healing in rats
narges nazari نسرین حیدریهPato physiological process of wound healing involves the interaction of cellular and biochemical processes. According to Ash anti-inflammatory effect Fraxinus extract the aim of this study was to evaluate the effect of Fraxinus extract ointment incisional wound healing MorePato physiological process of wound healing involves the interaction of cellular and biochemical processes. According to Ash anti-inflammatory effect Fraxinus extract the aim of this study was to evaluate the effect of Fraxinus extract ointment incisional wound healing in rats can be. Method: Ash fruits and leafy branches after identification by the university herbarium, extract by Soxhlet extraction using rotary powder was prepared.Then ointment 70% and 35% as a percentage of the volume using eucerin (ointment base) and the dried extract was created.To induce colitis in male Wistar rats first (g200 ± 230) were anesthetized with ether and In the middle of incisional wound to the back of the animals during 2 (cm) and the thickness of the dermis was created. Rats in four groups (n = 8), no treatment (negative control), eucerin treatment (positive control), treated with ointment were 35% and 70% Fraxinus extract .Treatment every other day for . 14 days was done Before treatment, the wound was photographed by a digital camera and then with the help of AutoCAD software and the percentage of wound healing, wound surface area was determined.Data analyzed by SPSS software and ANOVA and Tukey test were analyzed.The results showed ointment 35% and 70% Ash significant increase in the percentage of wound healing compared to the control group were positive.Result :Fraxinus extract extract is accelerated wound healing in rats. Manuscript profile -
Open Access Article
16 - A comparative study of the effects of hydroalcoholic extracts of purslane (Portulaca oleracea L.) and henna leaves (Lawsonia inermis L.) on wound healing in male Wistar rats
Fatemeh JamallouObjectives: One of the medicinal plants known in traditional medicine, henna with the scientific name Lawsonia inermis L. comes from the Lythraceae family and purslane with the scientific name Portulaca oleracea L. comes from the Portulacaceae family. According to the c MoreObjectives: One of the medicinal plants known in traditional medicine, henna with the scientific name Lawsonia inermis L. comes from the Lythraceae family and purslane with the scientific name Portulaca oleracea L. comes from the Portulacaceae family. According to the compounds present in these plants, many properties such as antimicrobial, anti-inflammatory, wound healing, trypsin inhibitory and antioxidant properties have been reported for them.Materials and Methods: This study was conducted on 24 male rats in the weight range of 200±20 grams, which were divided into 4 groups of six. Wounds 2 cm long were created on the back skin of mice: the first group was kept without treatment as a control, the second group was treated with Esrin (negative control) and the other two groups were treated with ointments made of henna and purslane with a concentration of 50% by weight. -Weight was used in the daily basis of Esrin. The area of the wound was measured daily from the second to the fourteenth day with Autocade software. The data were analyzed using SPSS software at a significance level of p<0.05.Findings: The decrease in wound area was significantly higher in all groups treated with purslane and henna. Purslane showed a better effect.Conclusion: Henna and purslane ointment can be used as effective substances in the treatment of skin wounds by significantly reducing the healing time and wound area. Manuscript profile -
Open Access Article
17 - Khaghani and Folk Beliefs About Animals
Hossein Zanganeh Iraj MehrakiKhaghani is one of the high level poets of Persian poetry and prose in the sixth century AH. He has been a man who has aristocracy on various topics, and he refers in his poems to various sciences. From the sociological point of view of literature, in which, nowadays co MoreKhaghani is one of the high level poets of Persian poetry and prose in the sixth century AH. He has been a man who has aristocracy on various topics, and he refers in his poems to various sciences. From the sociological point of view of literature, in which, nowadays considered by literary critics, Khaghani's poetry is full of social and political issues. One component of the sociology of literature is popular culture, or in other words, folklore. Khaghani's poetical work is full of folk beliefs of his era. Popular beliefs about animals can be found in the subset of popular culture. Surprisingly, some of these beliefs are applicable even now among the populace and properties.In this essay, the authors have attempted to examine, through a descriptive-analytical method, a section of Folklore in Khaghani's poetical work and to elucidate Khaghani's view of some superstitious material of his era. Manuscript profile -
Open Access Article
18 - Study Effects Of Hydroalcoholic Extract Of Medicago sativa in the Treatment Of Acetic Acid Induced Gastric Ulcer in Rats
Mojdeh Samari Mehdi Rahnema Shahrzad Nasiri Semnani Ali ShahnavazInroduction and Objective:Peptic ulcer, from histologicalpointisknownasmucosalnecrosisfactor. The purpose of this study was to investigate the effect of Alfalfa hydroalcoholicvegetivearial organs extract on gastric ulcer induced by acetic acid in Rat.Material and Method MoreInroduction and Objective:Peptic ulcer, from histologicalpointisknownasmucosalnecrosisfactor. The purpose of this study was to investigate the effect of Alfalfa hydroalcoholicvegetivearial organs extract on gastric ulcer induced by acetic acid in Rat.Material and Methods: In this experimental study, 60 wistar rats weighting approximately (200-250g) were randomly divided into 4 groups; control, sham(normal salin), experiment 1 (extract of dose 250 mg/kg body weight) and experiment 2 (extract of dose 500 mg/kg body weight). Rats were deprived of food and then underwent surgeryand gastric wasby injecting60% acetic acid after 4,7,10,14 days gavage, the rats' stomach out and then measuring the wound and fixed with 10% formalin, the passage of the tissue section was stained And the number of neutrophils, macrophages and fibroblasts were counted.Results:Results showed that the hydroalcoholic extract of Alfalfacaused a significant increase in gastric ulcer healing in experimental groups 1 and 2 (PConclusion:Alfalfahydroalcoholic vegetive arial organs extract is effective in the treatment of peptic ulcer induced by acetic acid. Manuscript profile -
Open Access Article
19 - Study Effects Of Hydroalcoholic Extract Of Melisa Officinalis in the Treatment Of Acetic Acid Induced Gastric Ulcer in Rats
تکرخ اشتری توان دشتی مهدی رهنما شهرزاد نصیری سمنانیInroduction and Objective:Peptic ulcer involved mucous extras and good heal under mucous in the wall of stomach. This decease usually is from the most known digest deceases, the plant drugs as compare with the chemical drugs had lower side effects and in contrast of che MoreInroduction and Objective:Peptic ulcer involved mucous extras and good heal under mucous in the wall of stomach. This decease usually is from the most known digest deceases, the plant drugs as compare with the chemical drugs had lower side effects and in contrast of chemical drugs that maybe had interfere with another compounds of body or produced a poison in the body, hasn’t interfere effects. Melisa officinalis have biologic effects such as calmness and antioxidant effects. In this research studied the effects of hydroalcoholic nectar of leaf ofMelisa officinalis plant over the peptic ulcer result of acid acetic on the RatMaterial and Methods: In this experimental study prepared hydraulic nectar of melisa officinalis plant. 60heads of female mousse in weight of 250-200 divided accidently to four 15groups, control group, Sham group (normal receiver of Salin) experiment1 group (extract of does 200 mg/kg) experiment2 group (extract of does 400 mg/kg). Mousse suffered form peptic ulcer through injection of acid acetic. After a day the rats treated for 14days through nectar process. Then the sample of stomach in the forth, tenth seventh and fourteen days excited and after measuring the sizes of wounds and fitting with formalin 10% , passage stages, performed tissue cut and coloring method and finally investigated the histology parameters.Results:the results showed that the nectar of Melisa officinalis result in meaningful increase of improving the wound in the experiment group 1 and 2 as compare with Sham and control groups, in the experiment group 2 improving the wound as compare with the experiment group 1 had meaningful increase. The inflammation factors and fibroblasts showed the meaningful decrease as compare with control and Sham group and showed meaningful reduction in the next days and the experiment group 2 showed meaningful reduction from froth day.Conclusion:Melisa officinalishas high recover ability in treatment of peptic ulcer result of acid acetic Manuscript profile -
Open Access Article
20 - Collagen Turnover in Healing of Cutaneous Wounds by Aloe vera Fresh Gel in Mouse: Involvement of Fibroblast Growth Factor Receptor
Mehran Arabi homeira JafarzadehInroduction & Objective: Using herbal products like Aloe vera (Aloe barbadensis Miller) plays an important role in wound healing. This study aimed to evalutea the curative influence of Aloe vera (AV) fresh gel on healing of open cutaneous wounds in mouse. Material MoreInroduction & Objective: Using herbal products like Aloe vera (Aloe barbadensis Miller) plays an important role in wound healing. This study aimed to evalutea the curative influence of Aloe vera (AV) fresh gel on healing of open cutaneous wounds in mouse. Material and Method: Mice were 1) pseudo-control (sham): wound treated with physiological serum; 2) wound treated with 2 gr AV fresh gel. Two equal full-thickness skin wounds were made on vertebral column in the sacral region. On 8th and 16th post wounding day, skin sampling for the purpose of gene expression of fibroblast growth factor receptor (FGF-R) under RT-PCR evaluation was taken place. Urine samples were used to determine the concentration of hydroxy proline (HP) as a marker of collagen turnover in wounds, using HP assay kit. Results: AV fresh gel could up-regulate the expression of FGF-R gene as compared to negative and pseudo-controls (P<0.05). In addition, HP concentration was incresed in AV gel-treated groups comparing to others. Conclusion: We revealed that AV fresh gel was capable of healing wounds via elevation in collagen turnover and represents a good candidate for a wound healing process in human and animals. Manuscript profile -
Open Access Article
21 - Green synthesis of zinc nanoparticles and investigation of changes in some biochemical parameters of blood and its effect on wound healing caused by burns in laboratory Wistar male rats
Zahra Sinaee pour Fard Nooshin Naghsh Nasrin Yazdanpanahi Gholam Reza AmiriIntroduction & Objective: Currently, zinc and its compounds are used as a suitable material for the treatment of infection in burns, open wounds and chronic wounds. This investigation, was designed and carried out with the aim the local effect of green zinc nanopart MoreIntroduction & Objective: Currently, zinc and its compounds are used as a suitable material for the treatment of infection in burns, open wounds and chronic wounds. This investigation, was designed and carried out with the aim the local effect of green zinc nanoparticles on blood biochemical parameters and skin tissue repair during wound healing in laboratory rat. Materials and methods: In this experimental study, 42 male Wistar rats were divided into 7 groups of 6. Then, by creating a 2 cm burn on the back of the rats, treatment was immediately performed with zinc nanoparticles ointment (1%, 4%, 16%) and zinc oxide ointment and pure Vaseline for 21 days. After sampling from the percentage of wound repair in the skin of rats was calculated. Similarly, blood sampling was done from the hearts of rats and serum was used to check biochemical parameters. Results: The percentage of wound healing in the 4% ointment group and zinc oxide ointment was significantly higher than the control group. In addition, all the concentrations of ointment were safe in terms of their effect on liver enzymes. Conclution: The results of investigation showed that the ointment of 4% zinc nanoparticles was the best treatment group, which accelerated the repair of skin wounds. Based on the results of the present experimental study, probably the ointment produced by the identified green nanoparticles could be suggested as a strong repair agent in skin lesions naturally. Manuscript profile -
Open Access Article
22 - The healing effect of earthworm’s hydro-alcoholic body extract on skin wound in goldfish (Carassius auratus gibelio)
Mehran Arabi AliReza ShamsAbadiBackground & aim: Wound healing is a complex programmed sequence of cellular and molecular processes. The objective of the present study was to evaluate the ability of earthworm’s hydro-alcoholic body extract (EBE) to heal the experimental skin wound in goldfi MoreBackground & aim: Wound healing is a complex programmed sequence of cellular and molecular processes. The objective of the present study was to evaluate the ability of earthworm’s hydro-alcoholic body extract (EBE) to heal the experimental skin wound in goldfish (Carassius auratus gibelio).Materials and Methods: Fish (independent triplicate, 14/group), for 14 days, were divided into 5 groups 1) negative control (Unwounded); 2) pseudo-control (sham): wound treated with EBE carrier (mixture of castor oil and white vaseline); 3) Positive control: wound treated with sodium phenytoin 1%; 4) 1 mg/gr EBE-treated wound, 5) 10 mg/gr EBE-treated wound. In this study, macroscopic and microscopic evaluations and collagen turnover (Hydroxyproline content) in the skin, along with determination of lysozyme activity in blood sera were carried out. Results: In fish in group 4 (1 mg/gr EBE-treated wound), an increase in the epithelium diameter, good presence and appropriate synthesis of collagen with and low edema in dermis; increased hydroxyproline content; and elevated blood lysozyme activity were observed comparing to other groups.Conclusion: Our results revealed that application of 1 mg/gr EBE might be as an effective medicinal material in the process of animal wound healing particularly in aquatics. Manuscript profile -
Open Access Article
23 - Effect of hydro-alcoholic extract of barberries (Berberis vulgaris) on wound healing process in rats
Saman Ahani Fariborz Moayer Nafiseh Khosravi DehaghiBackground and aim: Barberries are well known for their anti-inflammatory properties. The extract of this fruit may help accelerate epithelial tissue recovery; therefore, it could be considered as a potential substitute for synthetic medications. The current study aims MoreBackground and aim: Barberries are well known for their anti-inflammatory properties. The extract of this fruit may help accelerate epithelial tissue recovery; therefore, it could be considered as a potential substitute for synthetic medications. The current study aims to examine the impact of barberries hydro-alcoholic extract on open wound healing pathologic parameters and macroscopic size.Materials and methods: An incision was made in the lumbar region of twelve male rats under anesthesia. They were subsequently divided into treatment and control groups (n=6, each) and were treated with hydro-alcoholic extract of barberries-mixed with eucerin oil, and eucerin oil respectively for twenty-one days; i.e., once every 12 hours. In order to evaluate macroscopic changes in wounds, all rats were photographed daily. Furthermore, pathologic samples were obtained on the 1st, 3rd, 5th, 7th, 14th, and 21st days to investigate the parameters affecting wound healing.Results: The findings show that applying 150 mg/kg hydro-alcoholic extract of barberries has a significant effect on open wound healing in rats. The extract boosted angiogenesis, granulation tissue, epithelial thickness, fibroblast cells, and collagen production in the treatment group while dramatically reducing the macroscopic scar size and epithelial gap. Eventually, the healing skin was thoroughly replaced by normal skin tissue. Conclusion: Hydro-alcoholic extract of barberries (Berberis vulgaris) significantly accelerates the healing process of open wounds in male rats. Manuscript profile -
Open Access Article
24 - Investing effect of Malva , Thymus , Viola and Pinus, Extracts on Candida Glabrata in the In vitro and Animal model.
Seyedeh maryam Moosavinejad shahrzad Nasiri Semnani Rasol Shikri Reza ShapouriInroduction & Objective:The skin infected wound and wounds caused by burn are very epidemic in society, and these kind wounds rate treatment in effective to alleviate the patint, applying the herbal drugs with less complications to treat the wounds. Considering this MoreInroduction & Objective:The skin infected wound and wounds caused by burn are very epidemic in society, and these kind wounds rate treatment in effective to alleviate the patint, applying the herbal drugs with less complications to treat the wounds. Considering this significance , surveying Investing effect of Malva , Thymus , Viola and Pinus, Extracts on Candida Glabrata in the In vitro and Animal model. Material and Method:In this study, hydroalcholic extracts of Malva , Thymus , Viola and Pinus were prepared in the laboratory. Then, the MIC and MLC of the extract were determined by dilution method in the broth. In the study of the animal model, the bacteria were first inoculated with a concentration of (5×106CFU/ML) to the wound site on Syrian mice. After 24 hours, an ointment prepared with MLC concentration was prepared from extracts was used to treat burns and infections withCandida Glabrata. In the animal model, the extracts of have antimicrobial activity against growth of Candida Glabrata. Results: In this study, The MIC and MLC of the extracts model, the microbia were first inoculated with a concentration of (5×106CFU/ML) to the wound site on Syrian mice. After 24 hours, an ointment prepared with MBC concentration was prepared from extracts of the nettle, watercress and burdock root for 1 g of silver sulfadiazine and was used to treat burns and infections with Candida Glabrata. In the animal model, the extracts antimicrobial activity against growth of Candida Glabrata.In this case, the extract of Pinus and Viola have growth inhibitory Candida Glabrata activity stronger than Malva , Thymus extract. Conclusion: According to the findings of this study, it can be concluded that the extract of these 4 plants on an animal model has antimicrobial and healing effects on Candida Glabrata. It can be used as an anti-inflammatory agent orointment microbia to be raised. Manuscript profile -
Open Access Article
25 - Antifungal activity of nine plant extracts on tobacco Sore Shin disease (Rhizoctonia solani)
Sayed Afshin Sajjadi Gholam Reza Moradi Hoda Assemi Farhad Naghizadeh Faramarz Rostami Mohammad Akbarzadeh Mohammad Reza Najafi Zeinolabedin ShahadatimoghaddamTobacco Sore Shin disease, Rhizoctonia solani is one of the important agent with worldwide and causes yield losses in tobacco growing countries. This research has conducted with two targets, thestudy of antifungal activity of plant extracts on this pathogen MoreTobacco Sore Shin disease, Rhizoctonia solani is one of the important agent with worldwide and causes yield losses in tobacco growing countries. This research has conducted with two targets, thestudy of antifungal activity of plant extracts on this pathogen and screened the best solvent to extract. In this study, crude extracts of 9 plant species including: Catmint (Nepeta Cataria), tobacco (Nicotiana tabacum), thyme (Thymus pubescens) , Fennel (Foeniculum vulgar), Nepeta (Mentha pulegium) , hyssop (Hyssopus officinalis), Blam (Melissa angustifolious), Salvia (Salvia verticilata) and Badrashbi (Dracocephalum kotschyi) by using solvents, water, acetone , hexane, ethanol , and methanol were obtained. Antifungal activity of this compound, were tested using poisoned food method with concenterations of 0.1000 and 2000 ppm invitro landifious. Results indicated that crude extracts of Tobacco, Catmint, Thyme, Fennel, Hyssop and badrashbi have remarkable antifungal activity. Methanol was the best solvent to extract these compounds. Minimum inhibitory concentration of methanol extract of Tobacco, Catmint, Thyme, Fennel, badrashbi, and Hyssop were equal to 1.5, 1.5, 2, 3, 3, and 2.5 mg/ml, respectively when on this fungal pathogens. Therefore, it is likely possible to use plant extracts of these plants, especially N. cataria, N. tabacum and T. pubescens, for the control of tobacco sore shine disease. Manuscript profile -
Open Access Article
26 - Poems Collection Structures of "Hanjarey Zakhmi Taghazol (The Sore Larynx Lyricism)" of Hussein Monzavi
zivar dehghani shams- al -hajiyeh ardalani seyed ahmad kazerooniRepetition and circle are the main axes of the formation of musical elements. This pattern is also found in the musical system of modern poetry and covers three inner, outer and aside music areas. Based on these patterns, Monzavi has created the music of his poems. This MoreRepetition and circle are the main axes of the formation of musical elements. This pattern is also found in the musical system of modern poetry and covers three inner, outer and aside music areas. Based on these patterns, Monzavi has created the music of his poems. This study aims to examine the music creator arrays in sonnets of the collection "HANJAREY ZAKHMI TAGHAZOL" (sore throat lyricism) and the method of using these elements and also their function in Monzavi poems has been shown. The findings of this research suggest that Monzavi use different ways for the musical richness of his own lyrics including: pay attention to the diversity of prosodic rhythms, employing useful rhythms, create proper meaning and thematic with the rhythm of prosody, pun utilizing, repetition, contrast, rhymes musical function that make his poems unison, totally, Monzavi poem musicality elements classified in four categories: Outer music, aside music, internal music and spiritual music. Manuscript profile -
Open Access Article
27 - Comparing the effectiveness of cognitive therapy based on mindfulness and life therapy on cognitive flexibility and psychological capital in patients with dyspepsia without ulcers
Roghaieh Cheraghpoor Ramazan hasanzadeh Ghodratollah AbassiComparing the effectiveness of cognitive therapy based on mindfulness and life therapy on cognitive flexibility and psychological capital in patients with dyspepsia without ulcers یافتهها: نتایج نشان داد اثر اصلی گروه شناخت درمانی مبتنی بر ذهن آگاهی و زندگی درمانی معنا MoreComparing the effectiveness of cognitive therapy based on mindfulness and life therapy on cognitive flexibility and psychological capital in patients with dyspepsia without ulcers یافتهها: نتایج نشان داد اثر اصلی گروه شناخت درمانی مبتنی بر ذهن آگاهی و زندگی درمانی معنادار بود. با توجه به مجذر اتا میتوان گفت که 53 درصد تغییرات انعطافپذیری شناختی و 44 درصد تغییرات ناشی از اثر دو مداخله بود. همچنین بین میزان تاثیر دو روش شناخت درمانی مبتنی بر ذهن آگاهی و زندگی درمانی اختلاف معناداری وجود داشت. شناخت درمانی مبتنی بر ذهن آگاهی بر انعطاف پذیری شناختی و سرمایه روانشناختی تاثیر بیشتری داشت (01/0 >P). نتیجهگیری: با توجه به یافتهها نتیجه گرفته میشود شناخت درمانی مبتنی بر ذهن آگاهی و زندگی درمانی میتوانند بر بهبود بر انعطاف پذیری شناختی و سرمایه روانشناختی بیماران مبتلا به سوءهاضمه بدون زخم مؤثر واقع شوند. واژگان کلیدی: انعطافپذیری شناختی، سوءهاضمه بدون زخم، زندگی درمانی، سرمایههای روانشناختی، شناختدرمانی، ذهن-آگاهی. Manuscript profile -
Open Access Article
28 - Comparing the Effectiveness of Mindfulness-Based Cognitive Therapy and Life Therapy on Cognitive Flexibility and Psychological Capital in Patients with Dyspepsia Without Ulcers
Roghayeh Cheraghpour Khonakhdar Ramzan Hasanzadeh Ghodratollah AbbasiPatients experiencing dyspepsia without ulcers often encounter psychological challenges necessitating therapeutic interventions. This semi-experimental study aimed to compare the effectiveness of cognitive therapy based on mindfulness and life therapy on cognitive flexi MorePatients experiencing dyspepsia without ulcers often encounter psychological challenges necessitating therapeutic interventions. This semi-experimental study aimed to compare the effectiveness of cognitive therapy based on mindfulness and life therapy on cognitive flexibility and psychological capital in such patients. The research employed a pre-test and post-test design with a control group. The statistical population comprised individuals aged 25 to 50 diagnosed with indigestion without ulcers at Tuba Gastroenterology clinics in Sari and Ghaemshahr cities in 2022. A total of 45 participants were selected through available sampling, with 15 each in the experimental groups (mindfulness-based cognitive therapy and life therapy) and the control group. The experimental groups underwent eight sessions of therapy once a week for 90 minutes. Data were collected using the cognitive flexibility questionnaire by Dennis and Vanderwal (2010) and the psychological capital questionnaire by Loutans and Olive (2007) at pre-test, post-test, and a two-month follow-up. Mixed analysis of variance and Benferroni's post hoc test were applied for data analysis in SPSS version 24 software. Results indicated a significant main effect of both cognitive therapy groups, with 53% of cognitive flexibility and 44% of changes attributed to the interventions. Furthermore, a significant difference was observed between the effectiveness of mindfulness-based cognitive therapy and life therapy, with the former showing a greater impact on cognitive flexibility and psychological capital (P < 0.01). The findings suggest that both cognitive therapies based on mindfulness and life therapy can effectively enhance cognitive flexibility and psychological capital in patients with dyspepsia without ulcers.Keywords: Cognitive Flexibility, Dyspepsia without Ulcers, Life Therapy, Psychological Capital, Cognitive Therapy, Mindfulness.1. Manuscript profile -
Open Access Article
29 - The effect of combining mord (Myrtus communis) leaf extract and olive oil (Olea europaea) in comparison with 1% silver sulfadiazine cream on skin wound healing in Balb/c mice
Farkhondeh Nemati Ramin Ataee Fatemeh Gorji Seyed Taleb Houseini Amin Lotfvarzi Ali Bagheri hashem abadMord (Myrtus communis) is a plant with antibacterial and anti-inflammatory properties. Ointment of ethanolic extract of the leaves of the mord was prepared in olive oil in a ratio of 1: 1. Wounds developed in the full thickness of the skin in the dorsal region of the mi MoreMord (Myrtus communis) is a plant with antibacterial and anti-inflammatory properties. Ointment of ethanolic extract of the leaves of the mord was prepared in olive oil in a ratio of 1: 1. Wounds developed in the full thickness of the skin in the dorsal region of the mice. The mice were then divided into control and experimental groups. The control group did not receive any treatment and the experimental group, which included the group receiving the combination of mord leaf extract and olive oil and the group receiving 1% silver sulfadiazine cream, were treated once a day. Finally, the percentage of recovery, average skin thickness, diameter of hair follicles, collagen formation, and angiogenesis were examined on the fifth, tenth, and fifteenth days. Data were analyzed using a one-way ANOVA test using SPSS software version 22. The results showed that the treatment with the combination of ethanolic extract of mord leaf and olive oil led to a significant increase in wound healing percentage, skin thickness, hair follicle diameter, compared to 1% silver sulfadiazine cream. There was also an increase in collagen formation and angiogenesis. These results have been a reason for accelerating the wound healing process in the samples treated with a combination of mord extract and olive oil. The results of this study showed that the use of mord leaf extract and olive oil accelerates the transition from the inflammatory stage to the stage of fibroblast hyperplasia due to the increase in collagen synthesis and blood vessels in this stage. Manuscript profile -
Open Access Article
30 - Investigating the constituent chemical compounds and pharmacological effects of the medicinal plant Tanacetum parthenium
Hojjat Eghbal Mehdi Ahmadi SabeghAs living organisms, plants produce secondary metabolites as a means of adapting to various ecological conditions and phenomena around them in order to preserve themselves and future generations. Chamomile is a perennial and aromatic plant from the compositae family, wh MoreAs living organisms, plants produce secondary metabolites as a means of adapting to various ecological conditions and phenomena around them in order to preserve themselves and future generations. Chamomile is a perennial and aromatic plant from the compositae family, whose height reaches about 15-60 cm. With yellowish-green leaves and yellow flowers, the dried leaves or its aerial parts have been used in the treatment of headaches, menstrual irregularities, insect bites, inflammations and wounds, and fever since ancient times. The general name of this plant is actually derived from the modified Latin word febrifugia, and despite the fact that different therapeutic doses of cow chamomile plant have beneficial effects in the treatment of separate groups of patients, the usefulness of its clinical effects is not a matter of consensus. Cow chamomile is available as fresh leaves, powdered and dried leaves, capsules and tablets, liquid extract, standardized dry extract, crystals and edible drops. The variety of cautionary predictions associated with study design, either the appropriate concentration of the substance or the duration of evaluation from previous clinical studies, make it difficult to obtain the set of beneficial effects of this herb. Despite the different reports of the usefulness of this plant in patients who are looking for a suitable alternative to prevent migraines. Manuscript profile -
Open Access Article
31 - The effect of cold argon plasma in atmospheric pressure on increasing blood coagulation speed and full-thickness cutaneous wound healing in rats
mahsa Ale-Ebrahim Elham Janani Pejman MortazaviIn recent years, cold plasma in atmospheric pressure is used as a promising new therapeutic strategy in medicine for cessation of bleeding and skin wound healing. The aim of the present study was to evaluate the histopathological effect of cold argon plasma in atmospher MoreIn recent years, cold plasma in atmospheric pressure is used as a promising new therapeutic strategy in medicine for cessation of bleeding and skin wound healing. The aim of the present study was to evaluate the histopathological effect of cold argon plasma in atmospheric pressure on full-thickness cutaneous wound healing and increasing the speed of blood coagulation in rats. Forty-eight mature male rats were divided into two control and treatment groups. Under anesthesia, a 15 mm diameter skin wound was created on the back of the rats and blood coagulation time was measured. In treatment group immediately after the injury, the skin of rats was radiated by plasma once for 30 seconds and simultaneously blood coagulation time was measured. Then, control and treatment group rats were euthanized by ether at 0, 7 and 21 days after the injury respectively. The wound and surrounding healthy skin were removed and after hematoxylin-eosine staining, histopathological examination with light microscope was performed. Plasma radiation increased the speed of blood coagulation in skin wounds under in vivo conditions. Based on histopathological results, the process of full thickness cutaneous wound healing was significantly (p<0.05) better in plasma treated rats. Therefore, cold argon plasma can be used to increase the speed of blood coagulation and improve cutaneous wound healing. Manuscript profile -
Open Access Article
32 - Macroscopic evaluation of the effect of platelet rich fibrin on second intention cutaneous wound healing in the dog
Nosratollah Changizi داوود kazemiPlatelet rich fibrin is a platelet concentrate which could influence wound healing due to the release of growth factors. The purpose of this study was macroscopic evaluation of the effect of topical platelet rich fibrin on second intention cutaneous wound healing. For t MorePlatelet rich fibrin is a platelet concentrate which could influence wound healing due to the release of growth factors. The purpose of this study was macroscopic evaluation of the effect of topical platelet rich fibrin on second intention cutaneous wound healing. For this purpose, 14 adult male dogs were used. Four full thickness 2.5 × 2.5 cm wounds were created on the back of each animal either side of the vertebral column. The left and right side wounds were used as treatment and control respectively. Postoperatively, the percentage of wound contraction, epithelialization and wound healing were calculated on days 7, 14, 21 and 28 and the means were compared between the two groups using the independent sample t-test at 95% significance level. The results indicated that on day 28, percentage of wound contraction was insignificantly lower in the treatment group (44.03±6.15 vs. 49.26±10.09) while epithelialization was insignificantly higher (85.90±10.32 vs. 66.24±13.45) in comparison to the control group. Also the percentage of wound healing was significantly (p=0.03) higher in the treatment group compared with the control group (92.55±5.14 vs. 83.85±3.69). Overall, the results indicated that platelet rich fibrin improves macroscopic aspects of second intention cutaneous wound healing. Manuscript profile -
Open Access Article
33 - Experimental evaluation of Folic acid effect on Corneal burn ulcer healing in new Zealand white rabbit
Amirhossein Mahlojiyan alireza jahandideh Ahmad Asghari pezhman mortezaviBackground and Purpose: Corneal ulcer is one of the most common eye diseases, which can be caused by trauma, chemical agents, and some bacteria, viruses, and chlamydia. Treatment of corneal ulcer has been always a concern in ophthalmology. This concern is due to the maj MoreBackground and Purpose: Corneal ulcer is one of the most common eye diseases, which can be caused by trauma, chemical agents, and some bacteria, viruses, and chlamydia. Treatment of corneal ulcer has been always a concern in ophthalmology. This concern is due to the major role of cornea in providing and preserving normal vision. Alkali-induced corneal ulcer creates an intense inflammatory reaction to traumatic injuries and this intense inflammation can inhibit the natural epithelial growth and cause fibrosis or scar on cornea.Cornea is an organ in the eye that creates a smooth and clear surface and so, it provides the possibility of light regularly passing through the eye. Any injury of this layer eliminates its transparency and protective capacity. Cornea has few cells and no vessels. Various bacterial, viral, parasitic, and fungal agents can cause severe infections in cornea. After the occurrence of corneal injury, the cells surrounding the injured area including epithelial, stromal, and endothelial cells try to improve the injury by cell division and calling the immune system cells such as neutrophils, macrophages, and monocytes; as a result of this process, the injured area becomes inflamed and edematous. In most cases, due to basic membrane decay and leakage of proteinase, epithelial cells will not be able to provide the connections required for retrieving the layers. So, the distance between epithelial cells increase and the bond between them become weak. As a result of corneal ulcer, increased activity of destructive proteinases and decreased activity of proteases leads to rapid detachment of collagen and other extracellular components of corneal matrix. Significant increase of reactive oxygen species (ROS), increased oxidative condition, and decreased components of antioxidant system occur following corneal injury. In histological studies, various cells of immune system including neutrophils, lymphocytes, monocytes, and macrophages are found in cornea. Increased reactive oxygen species (ROS), increased oxidative stress, and decreased components of antioxidant system are observed in pathologic conditions and in the case of corneal injury. Various studies have reported the role of antioxidants in prevention and treatment of corneal ulcer. The recent studies have indicated that folic acid restores decreased enzymatic and non-enzymatic antioxidants. also, its improving effect is due to inhibition of reactive oxygen species (ROS). Materials and Methods: This study was carried out to investigate the effects of folic acid on corneal burn ulcer healing in 20 adult New Zealand white rabbits in similar weight and age range. The pure folic acid powder needed for this study was purchased from Sigma-USA Co. After general anesthesia, corneal ulcer was induced in the left eye of all rabbits and immediately fluoresce in staining was done to ensure that all of the ulcers were identical in size (6 mm). The rabbits were then divided into four equal groups including three experimental groups and a control group. After the surgery, the experimental groups (first to third groups) were gavaged with 2, 5 and 10 mg/kg body weight folic acid every day for 21 days. Histopathology At the end of the third week, the animals were anesthetized and the eye balls were harvested. After the eyeballs were isolated, the specimens were placed in 10% formalin. After the specimens were fixed in the laboratory, the corneal ulcer was isolated from eyeball. After preparation, the paraffin block sections of 4 microns were prepared and stained using Masson's trichrome staining method. In histopathological grading, the indices affecting wound healing, including angiogenesis, the absence of epithelial layers, corneal edema (stromal edema), irregularities in collagen filaments, and presence or absence of inflammatory cells were investigated. Results and conclusion: The histopathological studies showed that vascularization, inflammation, and corneal matrix edema were significantly reduced, but the epithelium of cornea was increased in folic acid treated rabbits compared to control group (p < /p> Manuscript profile -
Open Access Article
34 - Antibacterial effect of Capparis spinosa (Capparis spinosa) and Pistacia atlantica (Pistacia atlantica) extracts on growth of Escherichia coli in vitro and in vivo
Mohammad Sajad Ansari Cheharsughi Asiye Ahmadi-Dastgerdi Majid Gholami-AhangaranEscherichia coli is the cause of many surgical wound infections.This study was designed to investigate the antibacterial effects of hydroalcoholic extract of Capparis spinosa and pistacia atlantica on E. coli in surgical wounds using a rat model. The antimicrobial activ MoreEscherichia coli is the cause of many surgical wound infections.This study was designed to investigate the antibacterial effects of hydroalcoholic extract of Capparis spinosa and pistacia atlantica on E. coli in surgical wounds using a rat model. The antimicrobial activity of Capparis spinosa and pistacia altantica extract was first determined by paper disk diffusion method to determine Minimum Inhibitory Concentration (MIC) and also Minimum Bactericidal Concentration (MBC). Then, 30 male Sprague-Dawley rats were randomly divided into three treatment groups. A circular incision was made on the dorsal inter-scapular region of each rat. Then, rats were inoculated topically with 1.5×108 CFU of E. coli at the site of skin wounds. The extracts of Capparis spinosa and Pistacia atlantica was applied to wounds twice a day during the experiment. Animals of the control group were left untreated. The results showed that the extract of Caparis spinosa and Pistacia atlantica had antimicrobial effects against Escherichia coli. The antimicrobial activity of Capparis spinosa extract against E. coli was higher than the Pistacia atlantica extract (The MIC and MBC values). Also there was a significant difference in the rats treated with the extracts in comparison to the control group. It can be concluded that the extracts of these plants have antimicrobial properties and inhibit the growth of Escherichia coli in infected wounds and thus can accelerate the wound healing process. Conflict of interest: None declared. Manuscript profile -
Open Access Article
35 - Effect of Malva sylvestris hydroethanolic leaf extract on the healing of full-thickness, excisional skin wounds in the rat
mohammadreza Farahpour saro Sedaghat The healing of full-thickness wounds has always attracted attention due to disruption of the healing process that leads to prolonged recovery period. Therefore, the quest for finding clinically effective wound-healing agents is important in the medical mana More The healing of full-thickness wounds has always attracted attention due to disruption of the healing process that leads to prolonged recovery period. Therefore, the quest for finding clinically effective wound-healing agents is important in the medical management of full-thickness wounds. The present study was conducted to investigate the effects of Malva sylvestris hydroethanolic leaf extract on wound healing process in 54 male rats. Two standardized circular surgical full-thickness excisional wounds with a diameter of 7mm were created in the dorsal skin of vats in all three groups. The rats in the two treatment groups were treated with ointments containing 1.5% and 3% of the extract mixed with yellow soft paraffin, whereas the rats in the control group were only treated with yellow soft paraffin. Treatments were applied immediately after wounding and also for 16 consecutive days post-wounding. Wound-healing was assessed microscopically in terms of the initiation of neo-dermal tissue deposition and wound closure over a 16-day period. Significant increase in wound contraction rate was seen in the treatment groups, especially the 3% group, compared to the control group (P<0.05). Moreover, the inflammatory cell score showed a decrease, whereas new vessel formation, fibroblast distribution, collagen production and epidermis thickness increased in Malva sylvestris hydroethanolic leaf extract-treated animals. These results suggest that the topical application of Malva sylvestris hydroethanolic leaf extract has beneficial effects on full-thickness excisional wound healing. Manuscript profile -
Open Access Article
36 - A survey on the rate of abomasal ulcers of local cattle slaughtered in Noor city abattoir
seyyed mohsen mousavi saeed azimpour seyyed mohammad hosseini Abomasal ulcers are usually seen in dairy cattle with high production following the stress of parturition. Primary and secondary abomasal ulcers are seen in cows and suckling calves. Some ulcers are life-threatening, but the majority are without any clinica More Abomasal ulcers are usually seen in dairy cattle with high production following the stress of parturition. Primary and secondary abomasal ulcers are seen in cows and suckling calves. Some ulcers are life-threatening, but the majority are without any clinical signs and only diagnosed in slaughterhouse. The purpose of this study was to determine the occurrence of abomasal ulcers in local cattle slaughtered at noor city abattoir. For this purpose, the abomasum of 200 cows (92 bulls and 108 cows) aged 2.5 to 11 years (5.21±2.5) were investigated randomly after slaughter. For accurate diagnosis and grading of the lesions, tissue samples were prepared for histopathological investigation. Six cases (3%) of the examined abomasa had various types of abomasal ulcers. In this study, abomasal ulcer type 1 was observed with the highest frequency (3 cases). Type 2 and type 3 were seen in 2 and 1 case respectively. There was no type 4 abomasal ulcer. The highest number of ulcers was seen in pylorus and cardia. The highest percentage of abomasal ulcer was observed in cattle older than 9 years (4.77%). Significant difference was observed in the occurrence of abomasal ulcers in different ages (p<0.05). There was no significant association between sex and the incidence of abomasal ulcers. Manuscript profile -
Open Access Article
37 - The effect of topical application of autologous blood serum and platelet rich plasma mixture on clinical vision improvement after induction of experimental corneal stromal ulcers in rabbit
رامین Kaffash Elahi S.J Aledavood علی Rezaei Corneal ulcers which are mostly due to trauma are among the most common ocular disorders of animals that if not cured properly, might progress to blindness. In this research, the effects of platelet derived rich plasma (PRP) combined with autologous More Corneal ulcers which are mostly due to trauma are among the most common ocular disorders of animals that if not cured properly, might progress to blindness. In this research, the effects of platelet derived rich plasma (PRP) combined with autologous blood serum on vision improvement time (attainment of maximum corneal clarity) following creation of deep corneal stromal ulcer was evaluated. For this purpose, 25 adult male New Zealand which rabbits which were allocated to three groups of positive control (10 rabbits), negative control (5 rabbits) and treatment (10 rabbits) were used. In the control corneal regions of each animal, a deep stromal ulcer (extending from the corneal epithelium to the last stromal layer) with a diameter of 4mm was created by means of a probe. Then in the treatment group, pre-prepared PRP was placed immediately on the ulcer and autologous serum drop was administered as 2 drop, 3 times per day for 35 days. In the positive control group Chloramphenicol, Ciprofloxacin and Naphazoline ophthalmic drops and in the negative control group only distilled water were used in the same manner. During the 35 days of the study period, the visual status of the rabbits was evaluated daily. Based on clinical examinations, the treatment group achieved ideal vision significantly faster than the negative and positive control groups and the created ulcer had healed with the least amount of scar production (maximum light penetration from the cornea). Based on the results of this study, the used of PRP is recommended in the treatment of superficial to deep corneal ulcers to obtain faster heading, better vision improvement and least scar production. Manuscript profile -
Open Access Article
38 - Assessment of the effect of co- administration of Aloe vera gel and Fenugreek seed hydroethanolic extract on the improvement of full-thickness excisional skin wound healing in diabetic mice
mohamadreza farahpour mohammad Aghaei -
Open Access Article
39 - The effect of different concentrations of bee propolis on skin wound healing and immune response and survival of Common carp (Cyprinus carpio)
nasrin choubkar abbasali sari alireza bolandnazar hashem heshmati forough mohammadi nastaran shahbazian amirmohammad emamirad payam yari Antibiotics use to increase the immune and wound healing in many animals. But due to the residual effects of a drug, Antibiotics and used to increase the immune response and propolis wound healing is aquadic animals. But due to their residual, researchers a More Antibiotics use to increase the immune and wound healing in many animals. But due to the residual effects of a drug, Antibiotics and used to increase the immune response and propolis wound healing is aquadic animals. But due to their residual, researchers are looking to replace them with natural materials. One of these natural materials that has many health benefits is bee propolis. The aim of this study was to evaluate the effect of different concentrations of propolis on wound healing and immune system response in common carp (Cyprinus carpio). Propolis extracts were prepared from Kashan Barij essence with different concentrations of 0 , 2 , 5 and 10% with carrier substances (to dissolve the propolis in water), and were evaluated on wound healing and immune response and survival of common carp on a 21 –day cycle of short baths once a day . The results showed that, compared with the control group (0%), the use of bee propolis in concentration of 2% has statistically significant difference on wound healing and immune system response in common carp. The use of higher concentrations of propolis healed wound, but increased the number of blood cells (red blood cells, neutrophils, and eosinophils) and increased mortality of fish, so it is better to use lower concentrations of propolis in fish. The use bee propolis as additive in water is effective in low concentrations and stimulates the immune system. Manuscript profile -
Open Access Article
40 - Evaluation of the combined effect of St John's wort hydroethanolic flower extract and flaxseed oil on skin wound healing -in rats-
mohammadreza farahpour The use of biological substances is among the methods utilized to increase the rate and quality of wound healing. This experiment was designed to investigate the effects of co-administration of St John's wort hydroethanolic extract and flaxseed oil for acce More The use of biological substances is among the methods utilized to increase the rate and quality of wound healing. This experiment was designed to investigate the effects of co-administration of St John's wort hydroethanolic extract and flaxseed oil for acceleration of wound healing based on their therapeutic properties. The study was conducted on 72 male Wistar rats. Experimental wounds measuring 7 mm in diameter was created on both sides of animals then the animals treated with ointments once a day for 2 consecutive weeks. Wound area was determined on days 2, 4, 6, 8, 10, 12 and 14 using a transparent paper. Tissue samples were collected on days 3, 7 and 14 for histopathological studies. According to the data, hydroethanolic extract of St John's wort and flaxseed oil ointment decreased wound area and distribution of polymorphonuclear immune cells in wound area as well as increase in new vessel formation and fibroplasia (P<0.05). Furthermore, wound healing process was better in group treated with hydroethanolic extract of St John's wort and flaxseed oil ointment compared to the other groups. Manuscript profile -
Open Access Article
41 - Histometric and histopathological evaluation of the effect of Sertraline following cutaneous surgical trauma in the rat
سیامک Reyhani Rad داریوش Mohajeri Gh Mousavi جواد Mahmoudi علی Rezaie سعید YazdchiSelective serotonin reuptake inhibitors (SSRIs) are medications prescribed for the treatment of mood disorders including anxiety and depression. These drugs can prevent the development of inflammation and modulate interleukin and interferon production. It can be assumed MoreSelective serotonin reuptake inhibitors (SSRIs) are medications prescribed for the treatment of mood disorders including anxiety and depression. These drugs can prevent the development of inflammation and modulate interleukin and interferon production. It can be assumed from these features that the use of SSRIs could have a significant effect on wound healing. The aim of this experimental investigation was to evaluate the effect of sertraline on dermal wound healing in an animal model. In 60 Wistar rats, two circular wounds were made using a biopsy punch on the back of each animal. Rats were assigned to five groups of twelve animals each: Control group; Placebo group and experimental groups of the low, moderate and high dosage of sertraline ointment. Four rats of each group were sacrificed at 3, 7 and 15 days after operation. The shapes of the wounds were copied onto a transparent sheet, the figures were scanned, and the wound areas were calculated using scion image software and statistically analyzed. For histopathological studies, regenerated tissues were cut in the form of square pieces along with normal skin and stained with hematoxylin and eosin. The results show that sertraline treatment significantly reduced mean wound length. Morphmetrically, there were statistically significant differences between control, Placebo and experimental groups and there were significant histopathological differences between control, Placebo and experimental groups at the end of the study. The results of this study demonstrated that topical use of sertraline significantly improves healing of cutaneous wounds in the rat. Manuscript profile -
Open Access Article
42 - Effect of topical combination of flaxseed oil and alcoholic extract of Ceylon cinnamon in full-thickness surgical wound healing in a rabbit model
mohammadreza Farahpour majid ghafouriThe aim of the current study was to evaluate of wound healing activity of flaxseed oil and its combination with Cinnamon hydroalcoholic extract on excision of wound healing model in rabbit. Sixty New Zealand white rabbits were randomly allocated into 4 experimental grou MoreThe aim of the current study was to evaluate of wound healing activity of flaxseed oil and its combination with Cinnamon hydroalcoholic extract on excision of wound healing model in rabbit. Sixty New Zealand white rabbits were randomly allocated into 4 experimental groups. Two full-thickness skin excisional wounds were created on thoracolumbar region on each side of the dorsal midline in each rabbit. Rabbits in group 1 did not receive any treatments. In group 2, rabbits received placebo ointment; in group 3, animals were treated with flaxseed oil 2% ointment. In group 4, rabbits received ointment containing a combination of flaxseed 2%+Cinnamon hydroalcoholic extract 3%. Wound area (mm2) was determined on days 4, 8, 12, 16 and 20 using a transparent paper. Tissue samples were collected on days 3, 7, 14 and 20 for histopathological studies with Masson trichrome staining. According to the results, ointment containing a combination of flaxseed oil 2%+Cinnamon hydroalcoholic extract 3% significantly decreased wound area on all days after wounding in comporison with the control (p<0.05). Also, this combination ointment caused a significantly improved fibroblast distribution in treated animals compared to other groups (p<0.05). Our data showed that co-administration of flaxseed oil 2%+Cinnamon hydroalcoholic extract 3% ointment had beneficial effects on wound healing. Manuscript profile -
Open Access Article
43 - Evaluation of wound healing activity of Echinophora platyloba extract on experimental full thickness skin wound in the rat
ahmad asghari maryam Kardooni There are some specific plants used in traditional medicine, one of which is Echinophora platyloba. This study was conducted to evaluate the effects of platyloba (Echinophora platyloba) on the experimental full-thickness wound healing in rats. In this study More There are some specific plants used in traditional medicine, one of which is Echinophora platyloba. This study was conducted to evaluate the effects of platyloba (Echinophora platyloba) on the experimental full-thickness wound healing in rats. In this study 80 adult male Wistar rats were used. They were randomly divided into four groups including control, placebo, therapeutic ointment 1.5% and 3%. Animals were anesthetized and scrubbed from the shoulder to the ilium. Then, a circular wound was made with a diameter of 15 mm on the back between scapula and ischial tuberosity. The control group received no medication, while the placebo group received eucerin, the third and fourth groups received 1.5 and 3% Echinophora platyloba ointment locally. Histological samples were taken on days 3, 7, 14 and 21 in each group, then all samples were sent to the laboratory and processed with H&E staining method. Histopathological evaluations showed better healing results in those groups which had received Echinophora platyloba extract in comparison to other groups. Moreover, wound healing was significantly better in these groups (p<0.05). In conclusion, the results obtained from this study indicate that Echinophora platyloba extract had positive effect on wound healing in rat. Manuscript profile -
Open Access Article
44 - A Review of the Therapeutic Effects of Chamomile(Matricaria Chamomile) in Traditional and Modern Medicine
sepideh dolatiIntroduction: Research and study on the therapeutic effects of plants as well as on people's acceptance of these plants has been considered. Chamomile (Matricaria Chamomile) is one of the most widely used plants in traditional medicine. Chamomile is one of the medicinal MoreIntroduction: Research and study on the therapeutic effects of plants as well as on people's acceptance of these plants has been considered. Chamomile (Matricaria Chamomile) is one of the most widely used plants in traditional medicine. Chamomile is one of the medicinal plants for which various effects have been mentioned in traditional and modern medicine. In this article, studies on the therapeutic effects of Chamomile have been reviewed. Materials and methods: In this study, valid scientific articles, since 2000, on the pharmacological effects of Chamomile in Pubmed, ISI Web, Scopus, Google Scholar, SID and Magiran databaseshave been used, and Persian and English studies were intended in the search. Findings: In clinical and experimental studies, the therapeutic effects of this plant on the diseases of the gastrointestinal tract, neuropsychiatry, menstrual problems, anti-inflammatory, antiviral, antimicrobial, antioxidant, antispasmodic, analgesic and anticancer properties and wound healing have been proven. Conclusion: Matricaria Chamomile is recognized worldwide as an important medicinal plant. The various uses of this plant in the treatment of diseases from ancient times and its use in the pharmaceutical industry are the reason for its noticeable worthiness and valuable benefits in the world. Manuscript profile -
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45 - بررسی خاصیت ضد میکروبی عصاره تام و سه فراکشن Platychaeteaucheri (Boiss.) Boiss علیه 5 سویه استاندارد میکروبی و سویه های بالینی اشریشیاکلی
تینا ذبیحی نیک مژده حاکمی-والا فاطمه باقری بجستانیمقدمه و هدف:Platychaeteaucheri (Boiss.) Boiss متعلق به خانوادهAsteraceae بوده و یکی از گیاهان بومی ایران است. هدف از این مطالعه، بررسی خاصیت ضد میکروبی عصاره تام متانولی و سه فراکسیون حاصل از بخش هوایی گلدار Platychaete aicheri بر روی تعدادی باکتری گرم مثبت Moreمقدمه و هدف:Platychaeteaucheri (Boiss.) Boiss متعلق به خانوادهAsteraceae بوده و یکی از گیاهان بومی ایران است. هدف از این مطالعه، بررسی خاصیت ضد میکروبی عصاره تام متانولی و سه فراکسیون حاصل از بخش هوایی گلدار Platychaete aicheri بر روی تعدادی باکتری گرم مثبت و گرم منفی استاندارد، مخمر کاندیدا آلبیکنس و همچنین نمونههای بالینی اشریشیاکلی جدا شده از عفونت ادراری و زخم سوختگی است. روش تحقیق: عصاره تام متانولی Platychaeteaucheri با روش خیساندن تهیه شد. سپس فراکسیونهای کلروفرمی، اتردوپترولی و آبی با استفاده از روش استخراج مایع-مایع بدست آمدند. اثر ضدمیکروبی با روشهای انتشار در چاهک و براث میکرودایلوشن و بر اساس پروتکل CLSI مورد بررسی قرار گرفت. سویههای استاندارد مورد بررسی شامل: اشریشیاکلی (1399 PTCC)، سودوموناس آﺋروژینوزا (1430 PTCC)، استافیلوکوکوس ارﺋوس (1431 PTCC)، باسیلوس سرﺋوس (1247 PTCC) و کاندیدا آلبیکنس (5027 PTCC) بودند. همچنین عصاره و فراکسیونها بر روی سویههای اشریشیاکلی بالینی که از نمونه ادرار بیماران بیمارستان امام خمینی تهران که در سال 2013 و زخمهای سوختگی بیماران بیمارستان شهید مطهری تهران که در سال 2014 جدا شده بودند نیز مورد بررسی قرار گرفتند. نتایج و بحث: عصاره تام و فراکسیونهای کلروفرمی و اتردوپترولی دارای خاصیت ضدمیکروبی متوسط بر روی سودوموناس آﺋروژینوزا و اشریشیاکلی استاندارد با محدوده MIC (mg/ml 42-35) بودند. دامنه MIC برای ایزولههای بالینی اشریشیاکلی در محدوده mg/ml 72-60 بود. فراکسیون آبی فعالیت ضدمیکروبی کمتری نسبت به عصاره تام و سایر فراکسیونها بر روی نمونههای استاندارد و بالینی داشت. بر اساس یافتهها فراکسیونهای اتردوپترولی و کلروفرمی اثر آنتیباکتریال بیشتری نسبت به عصاره تام متانولی و فراکسیون آبی داشتند. پیشنهادهای کاربردی و صنعتی: بر اساس نتایج در صورت عدم سمیّت در مطالعات آتی، این گیاه میتواند به عنوان یک منبع آنتیباکتریال طبیعی در محصولاتی جهت کمک به درمان عفونتهای مجرای ادراری و سوختگی مورد استفاده قرار گیرد. Manuscript profile -
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46 - اثر ترمیم کنندگی زخم معده توسط عصاره ساقه و فراکسیون های Homalium Letestui
لوییس آمازو جواد اکوکون لوکی نوییدامقدمه و هدف: Homalium letestui که به طور سنتی توسط مردم ایبی بیو در جنوب نیجریه برای درمان زخم معده، مالاریا و سایر بیماریها استفاده می شود جهت بررسی ویژگیهای ممانعت کنندگی از ایجاد زخم معده مورد مطالعه قرار گرفت. روش تحقیق: تاثیر عصاره اتانولی ساقه (600-200 میلی Moreمقدمه و هدف: Homalium letestui که به طور سنتی توسط مردم ایبی بیو در جنوب نیجریه برای درمان زخم معده، مالاریا و سایر بیماریها استفاده می شود جهت بررسی ویژگیهای ممانعت کنندگی از ایجاد زخم معده مورد مطالعه قرار گرفت. روش تحقیق: تاثیر عصاره اتانولی ساقه (600-200 میلی گرم در کیلوگرم) و فراکشنها (آبی و دی کلرومتان؛ 400 میلی گرم در کیلوگرم) در زخم القا شده در شرایط آزمایشگاهی مورد مطالعه قرار گرفت. نتایج و بحث: عصاره (600-200 میلی گرم در کیلوگرم) از ایجاد زخم القا شده در مدلهای اتانول، ایندومتاسین، رزرپین و هیستامین به صورت وابسته به دوز ممانعت کرد و درجات ممانعت کنندگی از نظرآماری (p<0.05, 0.01, 0.001) معنی دار بودند. تاثیر عصاره قابل مقایسه با داروهای استاندارد مورد استفاده در مدلهای زخم القا شده توسط ایندومتاسین و اتانول بود و فراکشنهای به دست آمده توسط دی کلرومتان بیشترین فعالیت را داشتند. توصیه های کاربردی/صنعتی: این تحقیق نشان داد که عصاره ساقه Homalium letestui می تواند در درمان زخم موثر باشد. Manuscript profile -
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47 - بررسی هیستومورفومتریک تاثیر صمغ درخت کاج بر ترمیم زخم پوستی موش صحرایی نژاد ویستار
احمد روزبهانی الهام مقتدایی مصطفی نوربخشمقدمه و هدف :از مهمترین خواص درمانی ذکر شده برای گونههای مختلف رده کاج، خاصیت ضد میکروبی، خاصیت ضد التهابی، تسکیندهندگی، برطرف کننده­ی خارش­های پوستی، درمان کننده زخمها، جوشها و بثورات جلدی و بهبود اولسرها و زخمهای مزمن می باشد. در متون طبی کهن ایران از تر Moreمقدمه و هدف :از مهمترین خواص درمانی ذکر شده برای گونههای مختلف رده کاج، خاصیت ضد میکروبی، خاصیت ضد التهابی، تسکیندهندگی، برطرف کننده­ی خارش­های پوستی، درمان کننده زخمها، جوشها و بثورات جلدی و بهبود اولسرها و زخمهای مزمن می باشد. در متون طبی کهن ایران از ترکیبات قسمتهای مختلف انواع رده کاج به خصوص صمغ آن برای درمان زخمهای مزمن استفاده شده است. پژوهش حاضر به منظور بررسی اثر عصاره صمغ درخت کاج تهرانی بر روند ترمیم زخم پوستی در حیوان رت به اجرا درآمد. روش تحقیق : به همین منظور پس از عصارهگیری؛ پماد 40 درصد با پایه اوسرین از این نوع صمغ تهیه شد. پس از آن در مقام مقایسه 24 سر رت نژاد ویستار به 4 گروه 6 تایی تقسیم شدند.گروه دریافت کننده صمغ (گروه تجربی) گروه کنترل مثبت (پماد فنی توئین)،گروه کنترل منفی (گروه اوسرین) و گروه شاهد که تیماری بر روی آنها صورت نگرفت. سپس بر روی ناحیه کمری رت‏ ها زخمی با مساحت حدود 2 در 2 سانتیمتر پس از بیهوشی ایجاد شد. پس از ایجاد زخم عمل ضماد کردن پمادها تا 21 روز پس از برش انجام گردید. نتایج و بحث: در روز 3 ،7 و 21 پس از ایجاد زخم صفات مورفولوژیک و هیستوپاتولوژیک زخمها مورد بررسی قرار گرفتند. نتایج بررسی آماری مساحت زخم در روزهای مختلف حاکی از آن بود که در روزهای مختلف سطح زخم به صورت معناداری کاهش یافت(05/0>p) و مشخص شد که تاثیر فنی توئین و صمغ درخت کاج یکسان میباشد و کاهش زخم در این روش تفاوت معناداری نداشته است. از طرفی کاهش سطح زخم در هر سه روش آزمایش شده از گروه شاهد بیشتر بود. از نظر هیستوپاتولوژیک نیز در گروه صمغ درخت کاج نظم رشتههای کلاژن بهترین شکل و سلولهای التهابی در کمترین میزان خود بودند که احتمالا به دلیل اثر بر روی فیبروبلاستها میباشد. توصیههای کاربردی/صنعتی: با توجه به اثرات مثبت صمغ درخت کاج در التیام زخم می توان از آن در ساخت پمادهای سوختگی استفاده نمود. Manuscript profile -
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48 - اثر عصاره هیدروالکلی آویشن دنایی بر پیشگیری از زخم معده القا شده با اتانول در رت
فیروزه سقایی محمد صالحی محمد جواد نمازی محمد باقریمقدمه و هدف: از جمله بیماری های شایع در میان جوامع زخم معده است. درمانهای رایج این بیماری شامل داروهای کاهنده ترشح اسید هستند که ضمن اثربخشی دارای عوارض جانبی هستند. استفاده از گیاهان دارویی به عنوان کمک درمان این بیماری مورد توجه است. گیاه دارویی آویشن دنایی Thymus dae Moreمقدمه و هدف: از جمله بیماری های شایع در میان جوامع زخم معده است. درمانهای رایج این بیماری شامل داروهای کاهنده ترشح اسید هستند که ضمن اثربخشی دارای عوارض جانبی هستند. استفاده از گیاهان دارویی به عنوان کمک درمان این بیماری مورد توجه است. گیاه دارویی آویشن دنایی Thymus daenensis به دلیل داشتن ترکیبات متعدد من جمله فلاونوییدها می­تواند در پیشگیری از زخم معده موثر باشد. روش تحقیق: برای انجام این مطالعه از 35 موش صحرایی (200-250 گرم)در 7 گروه آزمایشی استفاده شد. گروههای آزمایشی شامل گروه سالین، گروههای دریافت کننده عصاره­ی آویشن دنایی با دوز 500 و 1000 و 1500 میلیگرم/کیلوگرم ، گروه امپرازول با دوز 20 میلیگرم/کیلوگرم، گروه کربوکسی متیل سلولز (حلال داروی امپرازول) به صورت خوراکی وگروه شاهد منفی دریافت کننده سالین خوراکی ، بودند. یک ساعت پس از درمان، با خوراندن 5/0 میلی لیتراتانول خالص به هر حیوان به جز گروه آخر زخم معده القا شد. پس از 4 ساعت، حیوانات بیهوش شدند، از قلب خونگیری صورت گرفت و بافت معده جدا گردید و جهت مطالعات هیستوپاتولو‍‍ژیک و بیوشیمیایی مورد استفاده قرارگرفت. میزان سوپراکسیددیسموتاز (SOD) سرم و میزان مالون دی آلدئید (MDA) بافت معده تعیین شد. نتایج و بحث: نتایج در گروههای مورد آزمایش نشان دهنده کاهش آلسر ایندکس وافزایش درصد مهار زخم و اثر محافظتی عصاره گیاه به صورت وابسته به دوز(در دوز بالاتر هم ارز اومپرازول) است. افزایش معنا دار میزان SOD و کاهش معنادار MDA نیز در اثر تجویز عصاره گیاه و اومپرازول بیانگر اثر مفید عصاره بر پایه آثار آنتی اکسیدان آن(کاهش استرس اکسیداتیو) می­باشد. توصیه کاربردی/ صنعتی: با توجه به نتایج حاصل می توان از آثار آنتی اکسیدان گیاه آویشن دنایی در درمان بیماریهایی که در اثر عوامل اکسیداتیو ایجاد می گردند در کنار داروهای دیگر بهره برد. Manuscript profile -
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49 - گلهای رز ایرانی، یک ضد باکتری طبیعی علیه عامل اصلی عفونت سوختگی پوست، سودوموناس آئروژینوزا
مائده رزقی سید رضا حسینی دوست ژینوس عسگرپناهمقدمه و هدف : رز ایرانی (Rosa foetida) متعلق به تیره گل سرخ می باشد و یکی از گونه های بومی ایرانی به حساب می آید که تاکنون اثرات بیولوژیک گل های آن بررسی نگردیده است. از آنجا که گل های این گیاه در طب سنتی ایران به عنوان مرهم جهت بهبود سوختگی های عفونی پوست به کار می رود Moreمقدمه و هدف : رز ایرانی (Rosa foetida) متعلق به تیره گل سرخ می باشد و یکی از گونه های بومی ایرانی به حساب می آید که تاکنون اثرات بیولوژیک گل های آن بررسی نگردیده است. از آنجا که گل های این گیاه در طب سنتی ایران به عنوان مرهم جهت بهبود سوختگی های عفونی پوست به کار می رود، بر آن شدیم تا برای اولین بار اثر ضد میکروبی عصاره های مختلف گل ها را علیه عامل اصلی عفونت های سوختگی، سودوموناس آئروژینوزا بررسی نماییم. روش تحقیق: بررسی اثر ضد میکروب عصاره ها و تعیین حداقل غلظت مهار رشد باکتری به ترتیب با استفاده از روش چاهک پلیت و روش رقت سازی متوالی در میکروپلیت انجام شد. نتایچ و بحث: تمام عصاره های حاصل از گل ها دارای اثر مهاری بر رشد باکتری بودند که از بین آنها عصاره های آبی و متانولی دارای قوی ترین اثر را نشان دادند. قطر هاله عدم رشد (با غلظت 125 میلی گرم/میلی لیتر) و حداقل غلظت مهار رشد باکتری برای هر دو عصاره فوق نسبتا با اثر داروی استاندارد، ایمیپنم/سیلاستاتین (8/8 میکروگرم/میلی لیتر) یکسان بود. توصیه کاربردی / صنعتی: نتایج مشاهده شده نشان می دهد که گل های رز ایرانی دارای ترکیبات موثره با اثر ضد باکتری علیه سوش های استاندارد و پاتوژن سودوموناس آئروژینوزا جدا شده از پنج بیمار مبتلا به عفونت سوختگی پوست است و کاربرد سنتی این گیاه را در عفونت های سوختگی پوست توجیه می نماید. Manuscript profile -
Open Access Article
50 - بررسی عملکرد رشد، خصوصیات لاشه، کیفیت بستر و وقوع جراحات پا در جوجههای گوشتی رشد یافته در تراکمهای بالای پرورش
د. فرهادی س.م. حسینیبه منظور بررسی اثرات تراکم­های مختلف بر عملکرد، خصوصیات لاشه، کیفیت بستر و وقوع جرحات پا دو آزمایش مکرر با استفاده از 5224 قطعه جوجه گوشتی یکروزه سویه راس 308 انجام گرفت. در هر آزمایش، تعداد 2612 قطعه جوجه گوشتی یکروزه در تراکم­های 16، 18، 20 و 22 قطعه در متر مر Moreبه منظور بررسی اثرات تراکم­های مختلف بر عملکرد، خصوصیات لاشه، کیفیت بستر و وقوع جرحات پا دو آزمایش مکرر با استفاده از 5224 قطعه جوجه گوشتی یکروزه سویه راس 308 انجام گرفت. در هر آزمایش، تعداد 2612 قطعه جوجه گوشتی یکروزه در تراکم­های 16، 18، 20 و 22 قطعه در متر مربع بستر با چهار تکرار به 16 پن آزمایشی مستقر در یک سالن مرغداری با سیستم کنترل شده محیطی اختصاص داده شدند. اطلاعات حاصل از هر دو دوره آزمایش برای تجزیه و تحلیل آماری با یکدیگر ادغام شدند. نتایج نشان داد که با افزایش تراکم در واحد سطح بستر، اضافه وزن بدن به طور معنیداری کاهش یافت (05/0>P). تراکم­های بالاتر از 16 پرنده در هر متر مربع بستر به طور معنی­داری موجب کاهش اضافه وزن بدن در دوره 1 الی 21 روزگی و تراکم بالاتر از 20 پرنده در هر متر مربع بستر در مقایسه با سایر تیمارها به طور معنی­داری موجب کاهش اضافه وزن بدن در دوره 22 الی 42 روزگی و کل دوره آزمایش گردید (05/0>P). وزن افزوده شده نهایی به شکل کیلوگرم وزن زنده در هر متر مربع بستر با افزایش تراکم به طور خطی افزایش یافت (05/0>P). تیمارهای آزمایشی فاقد اثرات معنی­دار بر ضریب تبدیل غذایی، میزان تلفات، شاخص کارایی تولید، صفات مربوط به لاشه (وزن لاشه، اوزان نسبی کبد، چربی حفره بطنی، بورس فابریسیوس و طحال) و اندازهگیری­های مربوط به بستر (رطوبت، pH و سطح آمونیاک) بودند (05/0<P). افزایش تراکم در واحد سطح منجر به افزایش معنی­دار در وقوع زخم کف پا شد (05/0<P)، در حالی که سوختگی مفصل خرگوشی تحت تأثیر افزایش تراکم قرار نگرفت (05/0<P). بر اساس نتایج به دست آمده از آزمایش، نتیجهگیری می­شود که تراکم 20 قطعه در هر متر مربع بستر، یک تراکم مناسب برای پرورش جوجه گوشتی در سالن واجد سیستم کنترل شرایط محیطی می­باشد. Manuscript profile -
Open Access Article
51 - Investigating the Effectiveness of Reality Therapy on Reducing Anxiety Caused by the Superstition of the Scarred Eye in People who refer to the Counseling Center in Mashhad
zahra vafaei jahan Belief in the wound causes people to feel anxious and insecure because they consider it out of their control. In this research, we will examine the effectiveness of reality therapy in reducing anxiety caused by believing in the injured eye. This study was quasi-ex More Belief in the wound causes people to feel anxious and insecure because they consider it out of their control. In this research, we will examine the effectiveness of reality therapy in reducing anxiety caused by believing in the injured eye. This study was quasi-experimental and was conducted using the pre-test and post-test method with the control group. 24 clients of Wafai Psychological Services and Counseling Office were selected by available sampling method and were randomly divided into two experimental and control groups. The measured instruments were the Beck-Rouan Anxiety Assessment Questionnaire. With this questionnaire, the level of anxiety of clients was measured. The results of the multivariate covariance analysis showed that the average anxiety scores of the experimental group in the post-test compared to the pre-test decreased significantly and also decreased significantly compared to the control group. In other words, reality therapy significantly reduced the level of anxiety of clients with superstitious beliefs about sore eyes. Manuscript profile -
Open Access Article
52 - Therapeutic effect of cold plasma on burnt skin in adult mice
Shadi Dorehlo simin mohamadi Gorji Nasim Hayati RodbariBurn wounds significantly heal the patient and these wounds are prone to infection with electrical energy and scar formation. The use of CO2 laser or nitrogen plasma along with the old methods of sterilization and whisper dressing to accelerate the process of blood clot MoreBurn wounds significantly heal the patient and these wounds are prone to infection with electrical energy and scar formation. The use of CO2 laser or nitrogen plasma along with the old methods of sterilization and whisper dressing to accelerate the process of blood clotting and wound healing. It may repair the natural process and be more effective than other treatments. Therefore, the aim of this study was to evaluate the effect of cold plasma on heat burn wound on the skin of adult Syrian mice in vivo. 24 adult male mice were randomly divided into control, fuel control, sham and experimental groups. After anesthesia and under sterile conditions, a circular wound with a diameter of 8 mm was created on the back of the mice's neck through hot body contact and caused burns as a zero day. Mice in experimental groups 1 to 3 were then treated daily for 30, 60 and 120 seconds, respectively, by cold or plasma irradiation of helium gas for one week. At the end of the treatment period, the sample bed was tested. The samples were tagged in Bowen and after molding with paraffin, 5 micron thick sections were prepared and stained with hematoxylin and stained. Wound healing is achieved by examining parameters such as the thickness of the stratum corneum, epidermis, dermis, hypodermis, the number of hair follicles and the diameter and number of blood vessels in the skin. Macroscopic examination of the wound site showed that the team of mice with cold plasma reduced the diameter of the burned area. Also, the thickness of skin layers (horny layers, epidermis, dermis and hypodermis) in experimental groups increased significantly (P <0.001) compared to the control group of fuel, as well as the number (P <0.001) and diameter of vessels. Dermal (P <0.01) was also increased compared to the control group of the fuel group. Wound area also decreased significantly (p <0.001) compared to the control group of fuel on the seventh day. The results showed that cold plasma irradiation of helium gas is effective in all performance indicators and by inducing it during burns can help accelerate wound healing. Manuscript profile -
Open Access Article
53 - Application of Nano Hydrogels In Improving The Performance Of Wound Dressings and Drug Delivery: A Review Study
MohammadHossein Karami Majid Abdouss MohammadReza Kalaee Omid MoradiOne of the most common clinical diseases is skin scarring, which is defined as damage to the structure or integrity of skin tissue. Nanohydrogel can help in sustained release of drug by entrapping drug or loading drugs and thus promote wound healing. A hydrogel is a thr MoreOne of the most common clinical diseases is skin scarring, which is defined as damage to the structure or integrity of skin tissue. Nanohydrogel can help in sustained release of drug by entrapping drug or loading drugs and thus promote wound healing. A hydrogel is a three-dimensional hydrophilic network that is insoluble in water or aqueous solutions and is able to absorb water or other biological fluids that can promote wound healing. Various nano hydrogels have been designed to improve the design of wound dressings, and nano hydrogels are also used in drug release. The advantage of hydrogels over traditional wound dressings is their ability to improve the wound and the wound healing process. Hydrogels meet most criteria for modern wound dressings due to their unique properties. Among the unique properties of nano-hydrogels, we can mention the ability to absorb wound secretions, maintain a moist environment, gas exchange, remove the dressing from the wound surface without pain and replace the dressing for the patient, ease of movement for surgery. In this research, an overview of the use of nano-hydrogels in improving the performance of wound dressings and drug delivery, along with the investigation of antibacterial properties, introduction of commercial wound dressings and wound healing mechanisms, has been discussed. Manuscript profile -
Open Access Article
54 - Design and manufacture of electrospun wound dressing based on polyvinylpyrrolidone fibers modified with aloe vera compounds for the treatment of skin edema
seyedeh Shadi Amini Dehkordi Nargess Aghamohamadi Javad Shabani ShayehIn this research, a new type of wound dressing based on polyvinyl pyrrolidone polymer (PVP) modified by extracted compounds from aloe vera (Av) was prepared by electrospinning method. After achieving optimal preparation conditions using experimental design method, the p MoreIn this research, a new type of wound dressing based on polyvinyl pyrrolidone polymer (PVP) modified by extracted compounds from aloe vera (Av) was prepared by electrospinning method. After achieving optimal preparation conditions using experimental design method, the prepared material was characterized using infrared Fourier transform spectroscopy, scanning electron microscopy, X-ray diffraction, differential scanning calorimetry analysis, and thermogravimetric analysis. The results of the studies showed that by using the optimal conditions, smooth filament of PVP without node with the diameter of about 100 nm can be achieved. PVP-Av and PVP-Av-Film fiber diameter as well as fibers with a special therapeutic coating were about 105 and 120 nm, respectively with a suitable surface to volume ratio. The results of the investigations showed that the use of polymer coating on the wound dressing not only increase its mechanical resistance, but also increase the hydrophilicity of this wound dressing. In addition, the results of biological investigations showed that this wound dressing has very little toxicity for biological cells and can be used to treat skin edema. Manuscript profile -
Open Access Article
55 - Struggle for Recognition: The Tragedies of "Rostam and Sohrāb" and "Oedipus the King"
حسین محمدصالحی دارانی The tragedies of "Rostam and Sohrāb" and "Oedipus the King" have certain points in common. There is a "struggle for recognition" in both. According to the legend of Shāhnāmeh, after years without any real knowledge of one another, Rostam and Sohr More The tragedies of "Rostam and Sohrāb" and "Oedipus the King" have certain points in common. There is a "struggle for recognition" in both. According to the legend of Shāhnāmeh, after years without any real knowledge of one another, Rostam and Sohrāb (father and son) face each other in battlefield, on the opposing sides. Rostam does not recognize his own son, and Sohrāb does not know his father. Likewise, in "Oedipus the King", on the road to Thebes, Oedipus meets Laius, his true father. Unaware of each other's identities, they quarrel over whose chariot has right-of-way. Oedipus's pride leads him to murder Laius. Manuscript profile -
Open Access Article
56 - Anti-microbial Effect of Nanoparticles on Non-Helicobacter Pylori Urease Positive Bacteria Isolated from Peptic Ulcer Patients
Jamile Nowrozi Mahtab Golmohamadi Ghadikolaii Farzane Hosaini Shahram Agah Siamak KhaleghiBackground and Objective: Recently the presence of several urease-positive bacteria other than Helicobacter pylori has been reported in gastric ulcer patients. The purpose of this study was the isolation and identification of urease-positive bacteria other than Helicoba MoreBackground and Objective: Recently the presence of several urease-positive bacteria other than Helicobacter pylori has been reported in gastric ulcer patients. The purpose of this study was the isolation and identification of urease-positive bacteria other than Helicobacter pylori in patients with gastric ulcer and at the same time, determining the anti-microbial effects of silver nanoparticles on the isolated bacteria. Materials and Methods: 50 gastric antrum biopsies were collected from patients with gastric ulcer who were admitted to the Rasoul Akram hospital (Tehran) by gastrointestinal specialists. The samples were transferred to the microbiology laboratory by transitive liquid medium. Urease-positive bacteria in the stomach were identified by standard bacteriological methods, including culture-specific and biochemical tests. The antimicrobial effects of the silver nanoparticles on urease-positive bacteria were determined according to minimum inhibitory concentration (MIC) and Minimum bactericidal Concentration (MBC) techniques. Results: The results showed that 42% of collected samples was urease-positive (10% Klebsiella pneumoniae, 10% Staphylococcus aureus, 8% Enterobacter cloace, 6% Enterobacter aglomerans, 4% Klebsiella azaene and 4%Citrobacter frondi). The antimicrobial effects of silver nanoparticles on the isolated bacteria showed 1.56-12.5 MIC and 3.125-25 MBC. Conclusion: Growth of urease-positive bacteria may lead to false positive observation on UBT and rapid urease tests. Therefore, it is better all urease-positive bacteria isolated from stomach to be sent for accurate diagnosis in order to improve the impacts of treatment. Also, in order to avoiding of bacterial resistance to antibiotics, silver nanoparticles are appropriate alternatives. Manuscript profile -
Open Access Article
57 - نشانگان اضطراب و افسردگی در بیماران مبتلا به زخم پپتیک
عباسعلی الهیاری حسین یوسفی کاظم رسول زاده طباطبایی پدرام طباطبایی -
Open Access Article
58 - وقوع ضایعات خورنده و پیشرونده ناحیه پرینه یک راس اسب نر نژاد کرد با انگل اکسیوریس اکوئی(oxyuris equi) در اطراف شهرستان تبریز
سید عبدالحمید حسینی سعید مستوفی محمد طلوعیآلودگی های انگلی یکی از متداولترین مشکلات پرورش اسب در جهان از جمله ایران می باشند. اکسیوریس اکوئی از انگل های گروه نماتودها بوده که دستگاه گوارش و نواحی اطراف مقعد در تک سمی ها درگیر می کند. در این مطالعه وقوع یک فرم غیر معمول اکسیوریازیس در یک اسب بالغ در تبریز گزارش Moreآلودگی های انگلی یکی از متداولترین مشکلات پرورش اسب در جهان از جمله ایران می باشند. اکسیوریس اکوئی از انگل های گروه نماتودها بوده که دستگاه گوارش و نواحی اطراف مقعد در تک سمی ها درگیر می کند. در این مطالعه وقوع یک فرم غیر معمول اکسیوریازیس در یک اسب بالغ در تبریز گزارش می شود. در پائیز سال 1397 یک راس اسب نر با نشانه های خارش و تظاهر برخی ضایعات در نواحی اطراف مقعد در یکی از اسبداریهای شهر تبریز مورد معاینه قرار گرفت. نشانه های حیاتی دام طبیعی بودند. زخم های مذکور در ناحیه پرینه و حتی زیر دم منتشر شده و یک زخم بزرگ پیشرونده و خورنده تشکیل شده بود. پس از جمع آوری نمونه های مدفوع و آزمایشات انگل شناسی تخم ها و حتی لارو انگل اکسیوریس اکوئی مورد مشاهده قرار گرفت. بر اساس مشاهدات بالینی و آزمایشات انگل شناسی درگیری به بیماری اکسیوریازیس مورد تائید قرار گرفت. درمان بیماری با تجویز داروهای ضد انگلی مثل پیرانتل اکسی بندازول و آیورمکتین شروع شد و همچنین ضد عفونی نواحی درگیر محلول های ید دار، تتراسایکلین و ویتامین آ و د انجام گردید. ضایعات مذکور در عرض 20 روز پس از درمان بیش از 90درصد بهبود یافته بودند. بر اساس این گزارش بیماری اکسیوریازیس می تواند در شکل غیر معمول بصورت زخم عمیق خورنده و پیشرونده در ناحیه پرینه حتی در اسب های بالغ ایجاد گردد. Manuscript profile -
Open Access Article
59 - اثربخشی تجویز خوراکی عصاره آبی بذر شنبلیله بر التیام زخم برشی در موشهای رت دیابتی
سید رزگار حسینی محمد رضا فرهپور سکینه نوری سعیدلودیابت، یکی از بیماری های غده ای در جهان است و التیام زخم را به تأخیر می اندازد. شنبلیله، دارای اثرات دارویی است. بنابراین این مطالعه با هدف بررسی کارایی تجویز خوراکی عصاره آبی بذر شنبلیله بر التیام زخم باز در مدل حیوانی آزمایشی دیابت انجام شد. استرپتوزوتوسین برای القای Moreدیابت، یکی از بیماری های غده ای در جهان است و التیام زخم را به تأخیر می اندازد. شنبلیله، دارای اثرات دارویی است. بنابراین این مطالعه با هدف بررسی کارایی تجویز خوراکی عصاره آبی بذر شنبلیله بر التیام زخم باز در مدل حیوانی آزمایشی دیابت انجام شد. استرپتوزوتوسین برای القای دیابت تجویز شد. یک زخم برشی با اندازه 6 میلیمتر روی ناحیه پشتی 24 موش صحرایی ایجاد شد. حیوانات به 4 گروه غیردیابتی، کنترل دیابتی و دیابتی تجویز خوراکی شده با 200 و 400 میلی گرم/کیلوگرم از عصاره شنبلیله تقسیم شدند. اندازه ناحیه زخم، پارامترهای بافت شناسی و غلظت سرمی انسولین و گلوکز خون در روزهای 3، 7 و 16 بررسی شدند. نتایج این مطالعه نشان داد که تجویز عصاره آبی بذر شنبلیله بطور معنی داری ناحیه زخم و غلظت گلوکز را در مقایسه با گروه کنترل از روز 7 کاهش داد (0/05>P). در مجموع، تجویز خوراکی عصاره آبی بذر شنبلیله قند خون را کاهش داده و باعث بهبود التیام زخم در موش های صحرایی دیابتی شد. Manuscript profile -
Open Access Article
60 - Morphological and histopatological study of Pratylenchus pseudocoffeae on Chrysanthemum in Mahallat region
E. Sedaghatfar A. Mohammad Deimi In order to identify the plant parasitic nematodes of Chrysanthemum in greenhouses and open fields in Mahallat region located in Markazi province in Iran, about 67 soil and root samples were collected during 2007 and 2008. The nematodes were ext More In order to identify the plant parasitic nematodes of Chrysanthemum in greenhouses and open fields in Mahallat region located in Markazi province in Iran, about 67 soil and root samples were collected during 2007 and 2008. The nematodes were extracted by centrifugal technique, killed, fixed and transferred to glycerine. Morphological and morphometrical characters were studied by light microscope. In our study, one of the most important nematodes was root-lesion nematode Pratylenchus pseudocoffeae. Root tissues were invaded by the nematode, which caused distinct necrosis and large cavities within cortical parenchyma. All developmental stages were observed in the root tissues. Morphometrics of P. pseudocoffeae is characterised by a labial region with three annuli, vulva located at 79-82% of body, long differentiated post-vulval uterine sac, tail sub hemispherical or bluntly pointed with smooth terminus, presence of males, spermatheca oblong. The species resembles P. coffeae, P. gutierrezi and P. penetrans from which it differs by a number of morphometric features (position of the vulva, the shape of the tail tip and head shape). This is a new occurrence and first record of the species in Iran and becomes the third world record after his original description. Manuscript profile -
Open Access Article
61 - Synthesis and Characterizatin of Polyvinyl Alcohol/Aloe Vera Hydrogel Film by Solvent Casting Method for Wound Dressing Applications
محبوبه محمودی مونا حاجیان رعنا ایمانیIn this study, we have tried to make hydrogel films by polyvinyl alcohol and Aloe vera which used in treat wounds. The films were prepared by solvent casting method with different concentration of aloe vera include 20, 30 and 50%. Some properties of samples such as wate MoreIn this study, we have tried to make hydrogel films by polyvinyl alcohol and Aloe vera which used in treat wounds. The films were prepared by solvent casting method with different concentration of aloe vera include 20, 30 and 50%. Some properties of samples such as water absorption, degradation rate, water vapour transmission rate and mechanical properties were examined. Also, Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) test were carried out in order to identify the bonds and morphological assessment in the samples, respectively. The results showed that with increasing of aloe vera concentration to 30% (PA30), water absorption rate, degradation rate and mechanical properties are increased. In according to SEM investigations, we found with aloe vera increasing, the porosity on surface of samples was increased. In the other hand, water vapour transmission rate have direct comparison with percentage of aloe vera concentration. Finally, cell cytotoxicity and morphology fibroblast cells (L929) attached on the samples surface evaluated by in vitro assay. The results showed that cells proliferated in greater numbers on the PA30 sample compared to other samples. Therefore, our results indicated that polyvinyl alcohol/Aloe vera hydrogel films are a good choice for use in wound dressing. Manuscript profile -
Open Access Article
62 - Restorative Effect of Iranian Probiotic Bacteria Lactobacillus casei on Healing gastric Stomach Ulcers Caused by Acetic Acid in Male Wistar Rats
اصغر سکوتی مهدی رهنما میترا حیدری نصرآبادی مریم ابراهیمی تاج آبادیGastric ulcer is a common disease caused by male nutrition and drug side effects. The treatment with chemical drugs is along with Physiological problems. Problems such as self -immunity has brought many efforts for finding new treatments for Gastric ulcer. Probiotic bac MoreGastric ulcer is a common disease caused by male nutrition and drug side effects. The treatment with chemical drugs is along with Physiological problems. Problems such as self -immunity has brought many efforts for finding new treatments for Gastric ulcer. Probiotic bacteria are the dominant digestive organisms used in the treatment of digestive diseases. One of the most important groups of probiotic bacteria is lactic acid, normally used as the ferment of dairy products The aim of this study is to investigate the restorative effect of probiotic bacteria isolated from native dairy products on healing gastric (stomach) ulcer of male wistar rats. In this study, the mice were randomly divided into three groups including the experimental, control 1 and control 2 groups. After tolerating Twenty-four hours of hunger, mice received surgery and Gastric (stomach) ulcer was created through injection of 12 / 0 ml of acetic acid (60%v/ v). One day after the surgery, the experimental group received 10 andtimes; 1010 cfu / day bacterial solution in one ml of sterilized milk, and control groups were respectively treated by one ml of sterilized milk and 2 ml of water through gavage until the time of dissecting. the mice were killed at 1, 4, 7, 10 and 14 days after building the stomach ulcer. The statistical analysis was performed by SPSS software and test results as Mean andplusmn; SEM were reported. The strains of bacteria Lactobacillus casei have significantly decreased the dimensions (sizes) of gastric (stomach) ulcer of the experimentalgroup than the control group 1 and 2 (p andlt; 0 / 001) and have increased gastric ulcer healing. Thehistological studies on the fourth day showed a significant increase of neutrophils, macrophages and fibroblasts in the experimental group than control groups and on the 7th, 10thand 14thday showed significant reduction of neutrophils and macrophages in the experimental group in comparison with control groups. Fibroblasts were significantly increased in the experimental group on 7thday and decreased in 10thand 14thday in comparison with control groups. The probiotic bacteria Lactobacillus casei have a significant restorative effect on gastric ulcer induced by acetic acid. Manuscript profile -
Open Access Article
63 - Evaluation of the Effect of Sedar (Ziziphus spina-christi) Extract on Biochemical Parameters of Skin Lesion in NMRI Mice
Shokooh Sharbatdar Nasim Hayati Roodbari Parichehr YaghmaeeThe skin is one of the largest organs of the body and acts as a protective barrier against external harmful factors including microorganisms. It can be damaged and sometimes if the damage is too much, the hypoderm is also involved, so more time is needed for restoration MoreThe skin is one of the largest organs of the body and acts as a protective barrier against external harmful factors including microorganisms. It can be damaged and sometimes if the damage is too much, the hypoderm is also involved, so more time is needed for restoration. The purpose of this research is to investigate cedar plant on skin wound healing in NMRI laboratory mice. 24 mice with a weight of approximately 25-35 grams were bought and after wounding the skin on days 3, 6, 12, 21 they were treated with ointment prepared from cedar and oserin. Mice were randomly divided into three groups: sham, experimental 1, experimental 2. These groups were treated with oserin and Cedar ointment with a dose of 2 mg and 4 mg respectively. Wounds were measured after 3 weeks and their tissue was evaluated by hematoxylin-eosin staining. Biochemical indices of SOD, MDA and TAC were also investigated. Data analysis was done by SPSS version 22 software and Tukey's test. The wound size in Cedar 1 and 2 groups had a significant decrease compared to oserin (p < 0.05). The concentration of MDA in cedar groups 1 and 2 had a significant decrease compared to oserin (p < 0.05). In addition, the activity of SOD and TAC were significantly increased in cedar groups 1 and 2 compared to oserin (p < 0.05). Also, there was a significant increase in the thickness of epidermis and dermis in cedar 1 and 2 compared to oserin (p < 0.05). There was a significant increase in the number of fibroblasts and blood vessels in cedar 1 and 2 compared to oserin (p < 0.05). According to the results of the present study, it can be concluded that cedar extract was effective in wound healing by increasing SOD, TAC and decreasing MDA. Manuscript profile -
Open Access Article
64 - The Effect of Ethanolic Extract of Quercus persica on Gastric Ulcer Healing in Rats
فهیمه مطیع میترا حیدری نصرآبادی عبدالحسین شیروی فریما زاهدیToday there is great interest in using traditional medicine for ulcer healing has been increased.Quercus persica is used in Iranian traditional medicine to treat some gastric diseases. The present study was undertaken to evaluate the Quercus persica ethanolic extract fo MoreToday there is great interest in using traditional medicine for ulcer healing has been increased.Quercus persica is used in Iranian traditional medicine to treat some gastric diseases. The present study was undertaken to evaluate the Quercus persica ethanolic extract for gastric ulcer healing in rats. Extractedfrom theskin of oakfruit soakedin90%ethanoland extractswere measuredby therotarydevice. To determine the effect of this extract from Quercus persica, 56 wistar male rats divided into 2 groups; experimental and control (n=7).Gastric ulcers were induced by luminal application of 0.12 ml acetic acid solution (40% v/v). One day after ulcer induction, the experimental groups Ethanolic extract of Quercus persica at concentration 100 mg/kg w.b.and the control groups received 1 ml normal salin through oral gavage for few consecutive days. On days 1, 3, 5 and 7 rats were killed and wound samples were collected for histological and statistical studies. Results analysis by one way Anova demonstrated that ethanolextract ofthe fruitskinof Quercus persica at concentration 100 mg/kg w.b.significantly increased gastric ulcer area compared to control group and decreased ulcer healing. Also, inflammation cells such as neutrophil and macrophage in the third and fifth days in the experimental groups compared with control groups had significantly increased, which is prolonged inflammation stage.On the seventh day, in the experimental groups a significant reduction in the amount of fibroblast was seen that indicates the delay in wound healing and collagen-synthesis process.The current study showedsignificant increase of inflammation and decrease wound healinginrats treated with ethanolextract ofthe fruitskinof Quercus persica at concentration 100 mg/kg w.b.as compared to control group Manuscript profile -
Open Access Article
65 - Effects of Lactobacillus casei Bacteria Killed by Chloroform on Cutaneous Wound Healing in Rats on Days 3 and 7
سیما مشایخ میترا حیدری نصرآبادی ویدا حجتی مریم تاج آبادی ابراهیمیUlcer healing means to retrieve the whole physical internal and external structures which includes the complex interactions between different cells and factors- plenty of researchers has paid so much attention to shorten the time of healing just due to cost reduction an MoreUlcer healing means to retrieve the whole physical internal and external structures which includes the complex interactions between different cells and factors- plenty of researchers has paid so much attention to shorten the time of healing just due to cost reduction and ulcer infection. They applied different ways to gain this goal so the basic aim of this research is to investigate the effects of cultivated Lactobacillas casei by chloroform on the Cutaneous woundhealing- we used 220-280 g rats- the rats were kept in 20andplusmn;2andCcedil; and food and water provided for the entirely then the rats were divided to five experimental- control and negative control groups. A quadrangle wound 1.5andtimes;1.5 was created. The control and experiment groups were treated topically oserin and the oserin contains cultivated Lactobacillus casei by chloroform but the negative control group has received no treatment rats skin ulcers in rats exposed to pathological sampling and statistical and histology surveying. Findings showed that there are significant differences between ulcer healing percentages of control and experimental groups and negative control. In next phases of healing in experimental group-comparing control and negative control group the number of notrofils reduced. According to findings Lactobacillus casei bacteria which cultivated in chloroform significantly result in decreasing inflammation and accelerating ulcer healing in rats. Manuscript profile -
Open Access Article
66 - The Effect of Extract of Henna Leaves (Lawsonia inermis) on Skin Wound Healing in Wistar Rats
عبدالحسین شیروی مهدی آل بویه ویدا حجتی حسن اکبریWound healingis one of the most basic human issues that are facing the beginning of creation. So trying to find the ingredients in the least adverse impact on the healing of human aspirations, especially in the use of medicinal plants can be important. Henna is native t MoreWound healingis one of the most basic human issues that are facing the beginning of creation. So trying to find the ingredients in the least adverse impact on the healing of human aspirations, especially in the use of medicinal plants can be important. Henna is native to Mediterranean regions, the Near East and India.Hana in Iran in areas south of the country, including Balochistan, Bam Nrmashyr grows. Mostpartsof matureleaves ofhennausedit.Since extracts of Henna (Lawsonia inermis L.) have anti-inflammatory and disinfectant effects, in this research, the effect of hydroalcoholic extract of this plant has been investigated on wound healing. After collecting and drying plant leaves, the hydroalcoholic extract soaking method were prepared and concentrated.Study of 48 male rats Wistar rats weighing 200-210 g were used in the range.Ulcers 3 cm in length on the back of mice were Haayjad and and then were divided into three groups of sixteen: The first group was kept as control without treatment, the second group of Eucerin(negative control), Group III of the ointment made from the henna plant extract based Eucerinwas used once a day. To study wound healing every day was measured during wound And the percentage of wound healing in different groups during all days of treatment based on one way ANOVA and Tukey test were compared. To histological studies, on days 3, 7, 14 and 21 of each group samples of full thickness wounds were removed.Average time of survey complete wound healing in group 3 (treated with henna extract) was lower than of a group 1 (no treatment) and group 2 (treated with Eucerin). Results indicate that the treatment group has the best effect in wound healing in comparison with other groups. It is possible that the components of henna have anti-inflammatory and antibacterial effects, and reduces inflammation, edema and bleeding and also stimulate collagen formation, faster wound contraction, angiogenesis and vasodilatation. Manuscript profile -
Open Access Article
67 - The Investigation of silymarin effect on colon ulcer induced acetic acid in mice Balb/C
وحید حمایت خواه جهرمی حبیب الله جوهریColonulcer is an inflammatory chronic colon disease and free radicals play an important role in its pathogenesis. Free radicals increase the severity of the disease by decreasing the antioxidants. Silymarin is a flavonoid component that itand#39;s extracted of Silybum m MoreColonulcer is an inflammatory chronic colon disease and free radicals play an important role in its pathogenesis. Free radicals increase the severity of the disease by decreasing the antioxidants. Silymarin is a flavonoid component that itand#39;s extracted of Silybum marianum. Silymarin contains antioxidants and inhibits the inflammatory reactions. The aim of this research is the investigation of anti- inflammatory effect of silymarin in the treatment of colon ulcer induced acetic acid in mice Balb/C spicies.In this study, 32 mice are divided into 4 groups (n=8).These groups include:control group with no colitis report, the Sham group with colitis report that are not treated and the groups with colitis report that received 10 20 mg/kg B.W. of silymarin orally.The treatment groups received silymarin daily, for 1 week after having the disease. The drugs are fed to the mice per oral using gaster tubes. To induce colitis, 1 ml of acetic acid (%4) is injected into the rectum. In the one week after induction of colitis, the animals sacrificed and the damages of the colon investigated in Morris and Murthy methods. The result of this study shown acetic acid causes severe inflammation and damages of the colon. Because colit ulcerative is an acute disease so, use of silymarin is effective in improving of the inflammation and repair of the colon ulcer induced acetic acid. So, use of silymarin can be considered as a way for the treatment. Manuscript profile -
Open Access Article
68 - The Effects of Chitosan/Polyvinilalchol Nanofibers Scaffold on Skin Burns in Adult Male NMRI Mouse
لیلا ریاحی نسیم حیاتی رودباری سهیلا سلحشور کردستانی کاظم پریورTo repair the wounds of burns a wide range of research including the use of bio-scaffolds due to ability to achieve the desired characteristics has been done. According to favorable biological properties of chitosan such as biocompatibility, antimicrobial properties and MoreTo repair the wounds of burns a wide range of research including the use of bio-scaffolds due to ability to achieve the desired characteristics has been done. According to favorable biological properties of chitosan such as biocompatibility, antimicrobial properties and the ability to improve wound healing in biomedical applications, and also due to biocompatibility and lack of stimulation of the immune system of polyvinyl alcohol, the authors decided to investigate the in-vivo effect of Nanofiber scaffolds on the mix of these two polymers on damaged skin.30 NMRI male mice were randomly divided in to a control group and three experimental groups (Scaffolding chitosan: polyvinylalcohol ratio of 0.100, 20.80, 30.70).Under sterile conditions and anesthesia, a 1 cm in diameter and full thickness skin wounds was created on the back of each mouse. Electrospinning nanofiber scaffolds were applied as wound dressing to cover the burn. The burn area was sterile every day and scaffolding was dressing on the wound. After 21 days histological studies have been done on the wound. The results showeda significant meaningful reduction in wound diameter in the experimental group in comparison to control group(p Also increase in the rate of wound shrinkage has been seen in samples containing scaffolds compared to control samples. Manuscript profile -
Open Access Article
69 - The Effect of Hydroalcoholic Extract Internal Layer of Iranian Oak (Quercus persica) on the Process of Wound Healing and Collagen Synthesis in Wistar Rats
Azadeh Kakoie Akbar karimi shima toghianiDue to their lower complications than chemical compounds, herbal medicines have received attention from doctors. In this study, Internal Layer of Persian Oak (Quercus persica), which is used in traditional medicine for chronic dermatologic disorders and eczema, wa MoreDue to their lower complications than chemical compounds, herbal medicines have received attention from doctors. In this study, Internal Layer of Persian Oak (Quercus persica), which is used in traditional medicine for chronic dermatologic disorders and eczema, was utilized to investigate cutaneous ulcer healing. Thirty adult male Wistar rats in 5 groups were treated while kept under normal laboratory conditions by creating a wound with dimensions of 1.5 × 1.5 cm2 and a depth of 2 mm in the back. The control group, the sham group, and the experimental groups consist of hydro-alcoholic extract of Internal Layer of Quercus persica at a dose of 250 mg / kg, hydro-alcoholic extract of Quercus persica at a dose of 500 mg / kg, and hydro-alcoholic extract of Quercus persica extract at a dose of 1000 mg / kg, respectively. Topical treatment of ulcers was performed in a 10-day interval every other day, and on the 6th, 8th and 10th days, tissue samples were prepared from the scars. After samples were washed, they were fixed in 10% formalin and hematoxylin-eosin staining of samples, epidermal thickness, wound healing percent, and collagen density were assessed and data were analyzed using SPSS software. In the experimental groups, there was a significant increase (at the level of p < 0.05 and p < 0.01) in the epidermal thickness and the wound healing percent, indicating the significant effect of Internal Layer of Iranian oak Jaft extract on the wound reconstruction, but no significant effect was observed on collagenization in this study Internal Layer of Iranian oak hydroalcoholic extract may have an effective role in accelerating skin wounds healing. Manuscript profile -
Open Access Article
70 - The Effect of Injection of Fibroblast Cells Isolated from Foreskin on Histological Improvement of Diabetic Wound in Animal Model
Abbas Zabihi Rahim Ahmadi Atefe DehghaniDespite a number of experimental studies, the healing effects of stem cells on chronic wounds still face serious challenges. The present study investigated the effect of injection of fibroblasts isolated from foreskin on the histological improvement of diabetic wounds i MoreDespite a number of experimental studies, the healing effects of stem cells on chronic wounds still face serious challenges. The present study investigated the effect of injection of fibroblasts isolated from foreskin on the histological improvement of diabetic wounds in male rats. 24 male Wistar rats were divided into control and treatment groups during this experimental laboratory study. Diabetes was induced in control and treatment groups using streptozotocin. Using a biopsy punch, a wound was created in the dorsal region of the animals Foreskin derived fibroblasts were injected into the dermis layer in the treatment group. On days 7, 14, and 21 after treatment, the wound healing was evaluated using morphologic observation, histological examination through hematoxylin- eosin staining, and measuring the wound size by Image j. On day 14 after treatment, the wound area was significantly smaller in the treated group than the control group (P<0.001). Histological examination showed that the skin thickness significantly increased in the treatment group on days 14 and 21 compared with control group (P<0.01). Besides, no morphologic complications were observed in the skin tissue following the injection of fibroblast cells. Our findings indicated that fibroblast cells are capable of accelerating the process of diabetic wound healing without morphologic complications in the skin tissue; according to which, the foreskin derived fibroblast cells can be used in the field of cell therapy. Manuscript profile -
Open Access Article
71 - Recent Biotechnological Advances in the Preparation of Wound Dressings for Diabetics
Sayed Rasoul Zaker Shahrbanoo Jafari Rahman EmamzadehIn the last two decades, given the importance of biotechnological drugs and their important role in the treatment of chronic wounds, studies have predicted that the market for advanced wound care targeting surgical wounds and chronic wounds is likely to reach $15-22 bil MoreIn the last two decades, given the importance of biotechnological drugs and their important role in the treatment of chronic wounds, studies have predicted that the market for advanced wound care targeting surgical wounds and chronic wounds is likely to reach $15-22 billion by 2024. Therefore, in the present study, the recent advances in biotechnology in the field of preparing wound dressings from compounds obtained from plants and animals for people with diabetic foot ulcers have been discussed. In this research, the methods and approaches of delivery of therapeutic agents for the treatment of DFUs and in fact the recent results of in vitro and in vivo studies, emphasizing the unique potential of wound dressings based on natural polymers in the treatment of DFUs, have been collected and analyzed. This expansion is expected to be driven by advances in technology, increased incidence of chronic wounds, and an increasing elderly population. A review of the challenges ahead in the treatment of chronic wounds reveals the inadequacy of the effectiveness of some wound care products, suggesting that families and governments must contend with medical challenges for longer periods. Diabetic foot ulcer (DFIU) is a chronic ulcer that can cause severe infections and even amputation. Biomaterials that are currently used as matrices for wound healing can be classified into natural and synthetic types according to their origin. Natural biomaterials such as chitosan, alginate, cellulose, hyaluronic acid, silk fibroin, and collagen have been widely recommended for diabetic foot wound healing due to their easy degradability, remarkable biocompatibility, reduced inflammatory responses, and non-immunogenic nature. Biotechnology guarantees the possibility of using natural biomaterials to develop advanced wound dressings with appropriate and patient-friendly therapeutic results. Manuscript profile -
Open Access Article
72 - Evaluation of the Effect of Ferula gummosa Extract on Biochemical Parameters of a Skin Wound in NMRI Mice
Parisa Seifali Parichehreh Yaghmaei Nasim Hayati RoodbariWound healing is one of the important problems in medicine. Therefore, this study aimed to investigate the effect of Ferula gummosa extract on skin wound healing in NMRI mice. 24 NMRI mice with skin ulcers, were treated with Ferula gummosa ointment on days 3, 6,12, and MoreWound healing is one of the important problems in medicine. Therefore, this study aimed to investigate the effect of Ferula gummosa extract on skin wound healing in NMRI mice. 24 NMRI mice with skin ulcers, were treated with Ferula gummosa ointment on days 3, 6,12, and 21, and randomly divided into three eucerin groups (daily recipient eucerin ointment), the experimental group1(daily treatment with Ferula gummosa ointment at a dose of 2 mg) and the experimental group 2 (daily treatment with Ferula gummosa ointment at a dose of 4 mg and the ulcers were measured with a calliper and the biochemical indexes of superoxide dismutase (SOD), malondialdehyde (MDA) and total antioxidant capacity (TAC) in tissue, were measured. Then, tissue sections were prepared from the wound and evaluated histopathologically by hematoxylin-eosin staining. The data were analyzed by SPSS 22 software and the Tukey test at a significance level of p < 0.05 and the histograms were drawn in excel. wound size in the Ferula gummosa groups compared to the Eucerin group showed a significant decrease (p < 0.05). The concentration of MDA in groups the Ferula gummosa significantly decreased time-dependent compared with the eucerin group (p < 0.05). SOD activity and TAC concentration also showed a significant increase in time-dependent in groups Ferula gummosa compared to the eucerin group (p < 0.05). Based on histopathological findings, epidermal and dermal thickness increased significantly in groups Barijeh compared to eucerin group (p < 0.05), and the number of fibroblasts and blood vessels in the two groups of Barijeh in a time-dependent manner compared to the Eucerin group showed a significant increase (p < 0.05). According to the results, it was shown that Ferula gummosa is effective in wound healing and repair by increasing SOD and TAC activity and decreasing MDA concentration, as well as histopathological results. Therefore, the findings of this study showed a new perspective on this herbal medicine for us wound healing. . Manuscript profile -
Open Access Article
73 - Protective Effect of Magnesium Sulfate against Experimental Models of Gastric Ulcer in NMRI Mice
Roya Rostami Akram Eidi Pejman Mortazavi Shahrebano OryanGastric ulcers are common digestive disorders involving stomach mucosal lesions with worldwide prevalence. There are different reasons for peptic ulcers. One common reason among west societies is ethanol consumption. The aim of the present study is pretreatment by magne MoreGastric ulcers are common digestive disorders involving stomach mucosal lesions with worldwide prevalence. There are different reasons for peptic ulcers. One common reason among west societies is ethanol consumption. The aim of the present study is pretreatment by magnesium sulfate. In this study 66 male NMRI mice were randomly divided into 11 groups and the administered mice were followed up daily for 15 days. These groups include control group (intact), control ulcer group (ethanol 10 mg/kg), standard group (omeprazole 40 mg/kg and ethanol), experimental groups (magnesium sulfate 50, 100, 200, 300 mg/kg), and experimental ulcer groups (magnesium sulfate 50, 100, 200, 300 mg/kg and ethanol). At the end of the relevant period, the levels of oxidative stress parameters were biochemically evaluated. The results of this study revealed that the activity of catalase, glutathione peroxidase and superoxide dismutase in control ulcer group significantly decreased compared to the control group. Oral administration of magnesium sulfate produced no significant effect on catalase, glutathione peroxidase and superoxide dismutase activities in intact animals. Animals in experimental ulcer groups represented a great increase in catalase, glutathione peroxidase, and superoxide dismutase levels over the treatment by magnesium sulfate. Moreover, our results showed that the amount of malondialdehyde in control ulcer group was significantly increased compared to the control group. Pre-treatment with magnesium sulfate caused no significant change on the amount of malondialdehyde in control group, while the level of malondialdehyde was considerably decreased in experimental ulcer groups. Furthermore, our results demonstrated that a pretreatment with MgSO4 could notably reduce oxidative stress and gastric lesions. Manuscript profile -
Open Access Article
74 - Identification Plant Parasitic Nematodes of Pistachio Orchards in Semnan province
Mahdi Mohammadi Moghadam Mohammad Niknam Masomeh Haghdel Mahdi NaeimiIdentification of alive Pathogenic factors and their successful control is one of the important challenges in pistachio production. In this research, 126 soil and root samples were collected from different pistachio growing areas of Semnan province in order to investiga MoreIdentification of alive Pathogenic factors and their successful control is one of the important challenges in pistachio production. In this research, 126 soil and root samples were collected from different pistachio growing areas of Semnan province in order to investigate and identify harmful plant nematodes. After transferring the samples to the laboratory, the nematodes were extracted by sieving and centrifugation, fixed using the completed degrease method and transferred to glycerin. Then, permanent microscopic slides were prepared from the nematodes separated by sex. After microscopic studies, morphological and morphometric characteristics were determined and identified using reliable scientific sources. In this study, 14 genera and species of plant harmful nematodes including Boleodorus thylactus, Criconemoides sp, Helicotylenchus dihystera, Helicotylenchus pseudorubustus, Irantylenchus sp, Meloidogyne incognita, Meloidogyne javanica, Pratylenchus thornei, Tylenchus devanei, Tylenchorhynchus sp, Rotylenchus sp, Zygotylenchus guevarai, Paratylenchus sp, Xiphinema sp was identified. Among the identified nematodes, the highest frequency of the population belonged to the two species M. incognita and M. javanica, Root-knot nematodes. These two types of nematodes were present in 35% of the collected samples. After Root-knot nematodes, the nematode Z. guevarai had the highest population density among the samples. This nematode has been reported as one of the internal migrant nematodes of the roots of dicotyledonous plants. But in this study, it was only separated from the soil. Investigating the damage caused by Root-knot nematode and Z. guevarai in pistachio orchards is one of the research priorities in Semnan province, and it is necessary to carry out more comprehensive investigations in relation to them. Manuscript profile -
Open Access Article
75 - An Investigation into the Effect of Nigella Sativa Oil on the Treatment of Gastric Ulcer Induced by Acetic Acid in Male Rats
Mahdieh AzarshabGastric ulcer or gastritis is a benign disease in the digestive system which is caused by a small ulcer in the inner lining and gastric mucosa. Among gastric ulcer characteristics is the deep ulcer that passes through the gastric mucosa. Almost all benign gastric ulcers MoreGastric ulcer or gastritis is a benign disease in the digestive system which is caused by a small ulcer in the inner lining and gastric mucosa. Among gastric ulcer characteristics is the deep ulcer that passes through the gastric mucosa. Almost all benign gastric ulcers are located in the anthrome (the lower stomach area). One of the methods to treat gastric ulcer is the use of medicinal herbs. Among effective medicinal herbs in treating gastric ulcer is Nigella sativa. The most important application of Nigella sativa is in inflammation reduction and wound healing acceleration. Substances such as linoleic oil, thymoquinone and antioxidants are obtained from nigella sativa. The rats were fed using a standard laboratory diet. Prior to surgery, the rats were deprived of food for 48 hours, and no water was given to them two hours before surgery. Gastric ulcer was generated by injecting acetic acid to stomach lining where there were no vessels. The oil at different doses was given to rats through gavage tube one day after gastric ulcer was generated. The rats were killed on days 4, 7, 10, and 14, and their stomachs were removed and the ulcer dimensions were calculated in terms of m2. Moreover, after cutting and providing lam, in order to conduct histological studies, samples were stained using hematoxylin and eosin and were examined by microscope. The obtained results showed that the percentage of wound healing in Nigella sativa at three doses (50, 100, 200 mg/kg) on days 4, 7, 10, and 14 had a significant increase in comparison to sham-operated and control groups (P<5%). Due to the presence of compounds such as terpenes, flavonoids, vitamins A, B, and C, and the anti-inflammatory and antioxidant properties in Nigella sativa, it facilitates the gastric ulcer healing, protects stomach tissue, and reduces gastric inflammation and ulcer, and has a greater restorative effect on gastric ulcer caused by acetic acid. Manuscript profile -
Open Access Article
76 - The Effects of Oil Extracted of Ricinus communis (Castor Oil Plant) on the Treatment of Gastritic Ulcer Induced by Acetic Acid in Male Rats
M. Azarshab gholamhassan vaezi M. RahnamaGastric ulcer is a benign disease in the digestion system caused by a small ulcer in the mucus layer of stomach. One of the ways to cure ulcer is using medicinal herbs. The medicinal herb, helpful in curing the gastric, is Ricinus communis. Its oil is antibacterial, ant MoreGastric ulcer is a benign disease in the digestion system caused by a small ulcer in the mucus layer of stomach. One of the ways to cure ulcer is using medicinal herbs. The medicinal herb, helpful in curing the gastric, is Ricinus communis. Its oil is antibacterial, antivirus and antifungal. It contains ricin oil, antioxidant, tanen and vitamin A, E and C that reduces inflammation of the ulcer. In this investigation, 60 male rats weighing 240-280 gr were provided. They were divided into 8 groups randomly, each including 12 rats. Control, sham and experiment group 1, 2, 3 with 50, 100, 200 mg/kg for Ricinus rats were not fed 48 h, 2h given water before surgery. Gastric was caused by injecting acetic acid to where there were no vessels. One day after causing the gastric, oil was given to the rats through gavage tube with different doses. The rats were killed in the day 4, 7, 10 and 14, and their stomachs were extracted and the dimensions of ulcer calculated based on millimeter square. After cutting samples and providing Lam with hematoxylin and eosin stain the percentage of recovery in oil of Ricinus in three doses (50, 100, 200 mg/kg) in the day 4, 7, 10 and 14 had a meaningful increase in comparison with Sham and control group (P<0.05). Manuscript profile -
Open Access Article
77 - The Effect of Cornus mas Extract on the Wound Healing of the Diabetic Male Wistar Rats
S. Alhooei-Nazari A. Shiravi Vida HojatiDiabetes is one of the reasons for delayed wound healing; as the fruit of blueberries, Cornus mas, possesses high content of antioxidants, therefore, this study aims to investigate the extract of this fruit on wound healing in a male rat of Wistar strain affected by dia MoreDiabetes is one of the reasons for delayed wound healing; as the fruit of blueberries, Cornus mas, possesses high content of antioxidants, therefore, this study aims to investigate the extract of this fruit on wound healing in a male rat of Wistar strain affected by diabetes.In this study, 48 male rats of Wistar strain were divided into 4 groups of 12. After affecting these groups to diabetes (except control group), there was created a wound at the back of the rat in all 4 groups; the length of the wounds in the groups were investigated up to 21 days; (1) control group: with no blueberries extract and ocerin treated on their wounds, called as healthy group; (2) Shem Group: they include a group of diabetics rats who were treated by ocerin (twice per day); (3) Experimental Group 1: they included a group of diabetics rats who received no ocerin and extract; (4) Experimental Group 2: they include the diabetics group who treated by the ointment of blueberries extract (twice a day).Results of comparing the mean of wounds length in the investigated groups indicate that the experimental group 2 had significant reduction up to p ≤ 0.01 than Shem and experimental1 groups. By reducing the blood glucose and modifying some biochemical factors, blueberries makes treatment effect on diabetes. According to the results of this study, blueberries ointment may accelerate skin wound healing in the healthy and diabetic samples. Manuscript profile -
Open Access Article
78 - Effects of Alcoholic Extract of Artemisia inflorescence on Wound Healing of Diabetic Wistar Rats
F. Harasani Gh. Vaezi Vida HojatiSkin ulcers and diabetic foot ulcers are an important issue in medicine. Diabetes is one of the most common metabolic diseases of the endocrine system. Artemisia pharmacological, particularly effects on diabetes. The aim of this study was to compare the healing effects MoreSkin ulcers and diabetic foot ulcers are an important issue in medicine. Diabetes is one of the most common metabolic diseases of the endocrine system. Artemisia pharmacological, particularly effects on diabetes. The aim of this study was to compare the healing effects of alcoholic extract of Artemisia in the process of healing and skin wounds in normal and diabetic rats is quickly closing. Overall, 20 adult male Wistar rats were used. The rats were divided into four groups of five controls (without diabetes and treatment), sham (diabetes treatment Eucerin), Experimental 1 (diabetes without treatment), Experimental 2 (diabetes treated with alcoholic extract of Artemisia) were divided. Three groups of rats were made diabetic using STZ. Over 2 cm wound on the skin of spinous were created in all groups. The experimental group and the experimental group 2 with wormwood extract 1 Eucerin day with a dose of 100 mg Barba topical treatment over a period of 21 d, respectively. Wound healing was evaluated by macroscopic and microscopic. Wound healing in experimental groups 1 and 2 was treated with than the alcoholic extract of Artemisia control group showed the highest rate. Wormwood extract leads to topical wound healing in diabetic rats and material contained in Artemisia cause, faster wound contraction, and the healing. Manuscript profile -
Open Access Article
79 - Comparative study of Teucrium extract and chlorhexidine in the healing and treatment of experimental oral ulcers
Pejman Nazem zomorodi loghman Akradi Amir Farhad Kochak Asa’adiBackground and Aim: The presence of any type of lesion in the mouth, such as injuries, sores or oral pests, in addition to causing pain in the area can cause the animal to not want to receive food as result, reduces weight and production. Teucrium is one of the medicina MoreBackground and Aim: The presence of any type of lesion in the mouth, such as injuries, sores or oral pests, in addition to causing pain in the area can cause the animal to not want to receive food as result, reduces weight and production. Teucrium is one of the medicinal plants that have effective healing properties in healing the mucosa. Therefore, the aim of this study was to compare comparative plant extract of Teucrium and chlorhexidine in the healing of experimental oral ulcers. Materials and Methods: In this experimental study, 36 New Zealand male rabbits were randomly divided into 3 equal groups: control without treatment (C), treatment group with 0.2% chlorhexidine (T1) and treatment group with hydroalcoholic extract of Teucrium (T2). In the beginning after anesthesia, the animals were placed in a supine position on the left side and the mouth of the animals was opened as much as possible by the mouth opener. Then, wound with a diameter of 3 mm was made on the gingival mucosa. Twice a day, the right gingival lesion was impregnated with a swab soaked in chlorhexidine solution (T1 group) and hydroalcoholic extract of Teucrium (T2 group). On days 1, 3, 7, and 14, the gums were sampled and the histopathological criteria for inflammatory cell infiltration, collagen filament arrangement, fibroblast cell presence, vascular formation, and epithelial tissue rearrangement were assessed. Also, the macroscopic diameter of the gingival tissue wound was measured on the mentioned days.Results: In T1 and T2 groups, the criteria for inflammatory cell infiltration, arrangement of collagen and vascular formation and rearrangement of epithelial tissue on days 7 and 14 showed a significant change compared to C group (P < 0.05). In the C group, the criteria of inflammatory cell infiltration increased during the study. In T1 and T2 groups, the macroscopic diameter of gingival tissue ulcer showed a significant decrease compared to C group (P < 0.05).Conclusion: This study showed that Teucrium extract can accelerate experimental wound healing. Manuscript profile -
Open Access Article
80 - Clinical and microscopic study of the effect of different concentrations of cardamom extract (Elettaria cardamomum) on the healing of skin wounds in diabetic rabbits with alloxan
Fatemeh Tavakoli Rad loghman Akradi Mahdieh RaeeszadehIn this study, 30 male rabbits weighing 900 - 1000 g were randomly divided into 6 groups (positive control, negative control and test). After general anesthesia using a combination injection of ketamine 50 mg / kg and xylazine 5 mg / kg intraperitoneally to create ulcer MoreIn this study, 30 male rabbits weighing 900 - 1000 g were randomly divided into 6 groups (positive control, negative control and test). After general anesthesia using a combination injection of ketamine 50 mg / kg and xylazine 5 mg / kg intraperitoneally to create ulcers in the rabbit skin a 10 mm circular punch was made. The wounds was measured with the aid of millimeter papers on the first, third, sixth, ninth, twelfth, fifteenth, eighteenth, and twenty-first days after surgery. This measurement is made at a specific time of day by a specific person. Complete recovery time was measured in negative, positive control and test samples. Then, on day 21, the wound was sampled. The specimens were subjected to histopathological studies after fixation of dewatering and staining.According to the results, there was no significant difference between control group and T1 group (P <0.05), but there was a significant difference between control group and T2, T3, T4, T5 group (P <0.05). The highest difference was between T5 and control group. It can be said that methanolic extract of cardamom at a dose of 400 mg / kg topical and feeding on cardamom powder pellets was more effective than all groups. Also, migration of epithelial tissue and reduction of wound openings in group T5 on days 15 to 21 were higher than other groups. Also, cardamom powder as the last plate of food significantly decreased glucose in diabetic groups (P <0.05). Cardamom lowers blood sugar by antioxidants such as flanoids. According to the results of this study, cardamom extract has a high ability to heal full-thickness wounds compared to chemical drugs. Manuscript profile -
Open Access Article
81 - Effect of Ketamine-Xylazine repetitive administration on open wound healing in rabbits- A clinical and histopathological evaluation
M. Abarkar S. M Foroud فریبرز Moayer مصطفی Darestani FarahaniOpen wound medication needs repeated chemical restriction for changing bandages in small animal medicine.combination of ketamine-xylazine is one of the most popular chemical restrain agents which decreases heart and respiratory rate and increase blood glucose.no previou MoreOpen wound medication needs repeated chemical restriction for changing bandages in small animal medicine.combination of ketamine-xylazine is one of the most popular chemical restrain agents which decreases heart and respiratory rate and increase blood glucose.no previous study was seen in literature review about systemic effects of this drug combination on wound healing.An experimental study was designed on twelve New Zealand white rabbits with spuare shape incisional wounds in the back region during the 21 day trial.rabbits were divided into two equal groups and bandage change was performed every 48 hours for both groups,one anesthetized with ketamine-xylazine combination during wound medication and other just physically restrained.heart and respiratory rate,O2 saturation of hemoglobin,and blood glucose were ,easured and recorded during each wound bandage.histopathological and macromorphological evaluations were accomplished by taking tissue sampling and photography of wounds.total wound,granulation and epithelial tissue area were measured by computer analysis for two groups as macromorphological evaluation. the data was anslyzed using SPSS(Version 16.00). heart and respiratory rates were decreased in rabbits which anesthetized during bandage change(p<0.05).but no significant difference was seen in O2 satruration of hemoglobin, and macromorphological measurmentes between the two groups.In descriptive and numerical comparisons between the groups no difference was seen in histopatological evaluation.the results of this study indicated that repeated anesthesia with ketamine-xylazine combination had no detrimental effect on wound healing. Manuscript profile -
Open Access Article
82 - Comparison of therapeutic effects of Platelet Rich Plasma Gel and Phenytoin on healing of full thickness skin wound in New Zealand white rabbit
, M Darestani Farahaani M. E Najafzadeh Khoei, فریبرز Moayer,Platelets Rich Plasma (PRP) technique, was introduced in 1987 and now is widely in use amongst in skin wound treatment . This study has been compared the effects of platelet-rich plasma and phenytoin on experimental fullthickness wounds in rabbits. A total of 15 health MorePlatelets Rich Plasma (PRP) technique, was introduced in 1987 and now is widely in use amongst in skin wound treatment . This study has been compared the effects of platelet-rich plasma and phenytoin on experimental fullthickness wounds in rabbits. A total of 15 healthy, adult male New Zealand rabbits with 1300 g (100 ± g) weight and 5 months (1 ± months) old age, were randomly divided in5 equal groups and were kept in individual cages. Heterologous PRP used in this study, was obtained via the collection of 10 mL blood from a mature New Zealand White male rabbits and centrifuged by two-steps procedure. Anesthesia were induced in rabbits by injecting of appropriate doses of xylazine and ketamine via the IM route , then performed pre-operative consideration ,and fnally a full-thickness wound with dimension of 1.5 × 1.5 cm created on both side of chest in all rabbits . Right and left wounds covered up with four drops of heterologous PRP and 0.5 Cm3 of phenytoin sodium (cream1%), respectively. During the 21-day period of the study, photographic images prepared from all wounds daily and then analyzed of wounds area and healing process, by Scion Image software. Under appropriate anesthesia in days of 3,5,7,14,21 after wound creation, histopathologic specimens were obtained from groups 1-5, respectively. The obtained data were analyzed in U MANN-Withney and T-Test. It was found different signifcance between days 14 to 21 only in wounds area and volume of granulation tissue in wounds treated with PRP v/s wounds treated with phenytoin sodium and there was no signifcant difference in other criteria’s (P <0.05). Manuscript profile -
Open Access Article
83 - Comparison of the two treatment protocols for wound myiasis in dogs referred to veterinary teaching hospital
S. Mohit Maf, S.Sh.R Shojaei,Wound discharge and necrotized tissue are attractive for flies to lay their eggs on wounds. Open wound myiasis occurs when the eggs convert to larva and they start to feed on debris and discharges of the wound. Complete removal of larva and controlling the infection ar MoreWound discharge and necrotized tissue are attractive for flies to lay their eggs on wounds. Open wound myiasis occurs when the eggs convert to larva and they start to feed on debris and discharges of the wound. Complete removal of larva and controlling the infection are the important steps for wound myiasis treatment. At least three days of hatching is required for converting the eggs to larva. Re-infestation of wounds may occur, even in a fly free environment, as the flies lay their eggs on different days. This study was conducted to compare the effectiveness of mechanical removal of maggots with or without application of Permethrin 5% Cream on 8 dogs suffering from wound myiasis. To evaluate the effectiveness of the two treatment protocols, re-infestation and wound healing were monitored on third, sixth and ninth days post treatment. Results showed that, although Permethrin 5% cream will prevent the re-infestation of wounds by larva, but have some adverse effects on wound healing. In comparison, mechanical removal of the larva without application of Permetrin will not prevent the re–infestation while provide an optimal wound healing process. According to the results, a minimum of two examinations with three days interval is necessary for the assurance of elimination of all larvas for wound myiasis treatment. Manuscript profile -
Open Access Article
84 - Histopathological effects of flowering shoot hydroethanol extracts of Hypericumperforatum on experimental full-thickness skin wound healing in rats
, M AsghariehAhary M.R Farahpour,Wound healing has always been of great concern for the surgeon and therefore using different effective biologicalsubstances of either plant or animal origin for a faster and more effcient wound healing (repair) has been considered.The aim of this study was to investigat MoreWound healing has always been of great concern for the surgeon and therefore using different effective biologicalsubstances of either plant or animal origin for a faster and more effcient wound healing (repair) has been considered.The aim of this study was to investigate the accelerating effects of Hypericumperforatumhydroethanolextracton open wound healing in rats. Study design: thirty-six adult male Wistar-albino rats (weight 200±10 g) wererandomly allocated to three groups (one control and two treatment groups). Two seven-mm, circular, fullthicknesses, skin wounds were created on the back of each rat (2 on the left and 2 on the right side). In the controlgroup, wounds were treated by an ointment with no extract. The treatment groups were treated by standardizedointment containing 1% and 2% Hypericumperforatum extract. For histopathological studies, the 3 groups weresubdivided into 4 subgroups with 4 rats in each. Samples were taken from the groups 1 to 4 on days 3, 7, 14, and21, respectively. After taking the sample, the rats were euthanized. The sequential wound biopsies were evaluatedhistopathologically for their heamorrage,neutrophils and lymphocyte, new vascularization and re epithelialization.According to the results of this study, the ointment with 2% extract was much better and slightly better than thecontrol and 1% extract ointments, respectively. Manuscript profile
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