Macroscopic evaluation of the effect of platelet rich fibrin on second intention cutaneous wound healing in the dog
Subject Areas :
Veterinary Clinical Pathology
Nosratollah Changizi
1
,
داوود kazemi
2
1 - Graduate of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
2 - Assistant Professor, Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
Received: 2017-07-27
Accepted : 2017-12-04
Published : 2018-01-21
Keywords:
Wound healing,
Dog,
Platelet rich fibrin,
Wound contraction,
Epithelialization,
Abstract :
Platelet rich fibrin is a platelet concentrate which could influence wound healing due to the release of growth factors. The purpose of this study was macroscopic evaluation of the effect of topical platelet rich fibrin on second intention cutaneous wound healing. For this purpose, 14 adult male dogs were used. Four full thickness 2.5 × 2.5 cm wounds were created on the back of each animal either side of the vertebral column. The left and right side wounds were used as treatment and control respectively. Postoperatively, the percentage of wound contraction, epithelialization and wound healing were calculated on days 7, 14, 21 and 28 and the means were compared between the two groups using the independent sample t-test at 95% significance level. The results indicated that on day 28, percentage of wound contraction was insignificantly lower in the treatment group (44.03±6.15 vs. 49.26±10.09) while epithelialization was insignificantly higher (85.90±10.32 vs. 66.24±13.45) in comparison to the control group. Also the percentage of wound healing was significantly (p=0.03) higher in the treatment group compared with the control group (92.55±5.14 vs. 83.85±3.69). Overall, the results indicated that platelet rich fibrin improves macroscopic aspects of second intention cutaneous wound healing.
References:
Barrientos, S., Brem, H., Stojadinovic, O. and Tomic‐Canic, M. (2014). Clinical application of growth factors and cytokines in wound healing. Wound Repair and Regeneration, 22(5): 569-578.
Bohling, M.W., Henderson, R.A., Swaim, S.F., Kincaid, S.A. and Wright, J.C. (2004). Cutaneous wound healing in the cat: a macroscopic description and comparison with cutaneous wound healing in the dog. Veterinary Surgery, 33(6): 579-587.
Borena, B.M., Martens, A., Broeckx, S.Y., Meyer, E., Chiers, K., Duchateau, L., et al. (2015). Regenerative skin wound healing in mammals: state-of-the-art on growth factor and stem cell based treatments. Cellular Physiology and Biochemistry, 36(1): 1-23.
Broughton, G.II, Janis, J.E. and Attinger, C.E. (2006). Wound healing: an overview. Plastic and Reconstructive Surgery, 117(7): 1e-32e.
Choukroun, J., Adda, F., Schoeffler, C. and Vervelle, A. (2001). An opportunity in perio-implantology: the PRF. Implantodontie, 42: 55-62.
Chignon-Sicard, B., Georgiou, C.A., Fontas, E., David, S., Dumas, P., Ihrai, T., et al. (2012). Efficacy of leukocyte-and platelet-rich fibrin in wound healing: a randomized controlled clinical trial. Plastic and Reconstructive Surgery, 130(6): 819e-829e.
Desai, C.B., Mahindra, U.R., Kini, Y.K. and Bakshi, M.K. (2013). Use of platelet-rich fibrin over skin wounds: modified secondary intention healing. Journal of Cutaneous and Aesthetic Surgery, 6(1): 35-37.
Dohan, D.M., Choukroun, J., Diss, A., Dohan, S.L., Dohan, A.J.J., Mouhyi, J., et al. (2006). Platelet rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology, 101: e37-e44.
Dohan Ehrenfest, D.M., de Peppo, G.M., Doglioli, P. and Sammartino, G. (2009). Slow release of growth factors and thrombospondin-1 in Choukroun’s platelet-rich fibrin (PRF): a gold standard to achieve for all surgical platelet concentrates technologies. Growth Factors, 27: 63-69.
Dohan Ehrenfest, D.M., Lemo, N., Jimbo, R. and Sammartino, G. (2010). Selecting a relevant animal model for testing the in vivo effects of Choukroun's Platelet-Rich Fibrin (PRF): Rabbit tricks and traps. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology, 110: 414-416.
Dohan Ehrenfest, D.M., Rasmusson, L. and Albrektsson, T. (2008). Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte-and platelet-rich fibrin (L-PRF). Trends in Biotechnology, 27(3): 158-167.
Enoch, S. and Leaper, D.J. (2008). Basic science of wound healing. Surgery (Oxford), 26(2): 31-37.
Ghamsari, S.M., Dehghan, M.M., Rassoli, A. and Nowrouzian, I. (2001). Clinical evaluation of chitin and chitosan effects on lower limbs open wound healing in horses. Journal of the Faculty of Veterinary Medicine, University of Tehran, 56(2): 1-7. [In Persian]
Halloran, C.M. and Slavin, J.P. (2002). Pathophysiology of wound healing. Surgery (Oxford), 20(5): i-v.
Lee, H.W., Reddy, M.S., Geurs, N., Palcanis, K.G., Lemons, J.E., Rahemtulla, F.G., et al. (2008). Efficacy of platelet-rich plasma on wound healing in rabbits. Journal of Periodontology, 79(4): 691-696.
Pinto, N.R., Ubilla, M., Zamora, Y., Del Rio, V., Dohan Ehrenfest, D.M. and Quirynen, M. (2017). Leucocyte-and platelet-rich fibrin (L-PRF) as a regenerative medicine strategy for the treatment of refractory leg ulcers: a prospective cohort study. Platelets, 1-8.
Reinke, J.M. and Sorg, H. (2012). Wound repair and regeneration. European Surgical Research, 49(1): 35-43.
Robson, M.C., Steed, D.L. and Franz, M.G. (2001). Wound healing: biologic features and approaches to maximize healing trajectories. Current Problems in Surgery, 38(2): 72-140.
Singer, A.J. and Clark, R.A. (1999). Cutaneous wound healing. New England Journal of Medicine, 341(10): 738-746.
Sorg, H., Tilkorn, D.J., Hager, S., Hauser, J. and Mirastschijski, U. (2017). Skin wound healing: an update on the current knowledge and concepts. European Surgical Research, 58(1-2): 81-94.
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Barrientos, S., Brem, H., Stojadinovic, O. and Tomic‐Canic, M. (2014). Clinical application of growth factors and cytokines in wound healing. Wound Repair and Regeneration, 22(5): 569-578.
Bohling, M.W., Henderson, R.A., Swaim, S.F., Kincaid, S.A. and Wright, J.C. (2004). Cutaneous wound healing in the cat: a macroscopic description and comparison with cutaneous wound healing in the dog. Veterinary Surgery, 33(6): 579-587.
Borena, B.M., Martens, A., Broeckx, S.Y., Meyer, E., Chiers, K., Duchateau, L., et al. (2015). Regenerative skin wound healing in mammals: state-of-the-art on growth factor and stem cell based treatments. Cellular Physiology and Biochemistry, 36(1): 1-23.
Broughton, G.II, Janis, J.E. and Attinger, C.E. (2006). Wound healing: an overview. Plastic and Reconstructive Surgery, 117(7): 1e-32e.
Choukroun, J., Adda, F., Schoeffler, C. and Vervelle, A. (2001). An opportunity in perio-implantology: the PRF. Implantodontie, 42: 55-62.
Chignon-Sicard, B., Georgiou, C.A., Fontas, E., David, S., Dumas, P., Ihrai, T., et al. (2012). Efficacy of leukocyte-and platelet-rich fibrin in wound healing: a randomized controlled clinical trial. Plastic and Reconstructive Surgery, 130(6): 819e-829e.
Desai, C.B., Mahindra, U.R., Kini, Y.K. and Bakshi, M.K. (2013). Use of platelet-rich fibrin over skin wounds: modified secondary intention healing. Journal of Cutaneous and Aesthetic Surgery, 6(1): 35-37.
Dohan, D.M., Choukroun, J., Diss, A., Dohan, S.L., Dohan, A.J.J., Mouhyi, J., et al. (2006). Platelet rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology, 101: e37-e44.
Dohan Ehrenfest, D.M., de Peppo, G.M., Doglioli, P. and Sammartino, G. (2009). Slow release of growth factors and thrombospondin-1 in Choukroun’s platelet-rich fibrin (PRF): a gold standard to achieve for all surgical platelet concentrates technologies. Growth Factors, 27: 63-69.
Dohan Ehrenfest, D.M., Lemo, N., Jimbo, R. and Sammartino, G. (2010). Selecting a relevant animal model for testing the in vivo effects of Choukroun's Platelet-Rich Fibrin (PRF): Rabbit tricks and traps. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology, 110: 414-416.
Dohan Ehrenfest, D.M., Rasmusson, L. and Albrektsson, T. (2008). Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte-and platelet-rich fibrin (L-PRF). Trends in Biotechnology, 27(3): 158-167.
Enoch, S. and Leaper, D.J. (2008). Basic science of wound healing. Surgery (Oxford), 26(2): 31-37.
Ghamsari, S.M., Dehghan, M.M., Rassoli, A. and Nowrouzian, I. (2001). Clinical evaluation of chitin and chitosan effects on lower limbs open wound healing in horses. Journal of the Faculty of Veterinary Medicine, University of Tehran, 56(2): 1-7. [In Persian]
Halloran, C.M. and Slavin, J.P. (2002). Pathophysiology of wound healing. Surgery (Oxford), 20(5): i-v.
Lee, H.W., Reddy, M.S., Geurs, N., Palcanis, K.G., Lemons, J.E., Rahemtulla, F.G., et al. (2008). Efficacy of platelet-rich plasma on wound healing in rabbits. Journal of Periodontology, 79(4): 691-696.
Pinto, N.R., Ubilla, M., Zamora, Y., Del Rio, V., Dohan Ehrenfest, D.M. and Quirynen, M. (2017). Leucocyte-and platelet-rich fibrin (L-PRF) as a regenerative medicine strategy for the treatment of refractory leg ulcers: a prospective cohort study. Platelets, 1-8.
Reinke, J.M. and Sorg, H. (2012). Wound repair and regeneration. European Surgical Research, 49(1): 35-43.
Robson, M.C., Steed, D.L. and Franz, M.G. (2001). Wound healing: biologic features and approaches to maximize healing trajectories. Current Problems in Surgery, 38(2): 72-140.
Singer, A.J. and Clark, R.A. (1999). Cutaneous wound healing. New England Journal of Medicine, 341(10): 738-746.
Sorg, H., Tilkorn, D.J., Hager, S., Hauser, J. and Mirastschijski, U. (2017). Skin wound healing: an update on the current knowledge and concepts. European Surgical Research, 58(1-2): 81-94.
