The Effect of Hydroalcoholic Extract Internal Layer of Iranian Oak (Quercus persica) on the Process of Wound Healing and Collagen Synthesis in Wistar Rats

Subject Areas : Journal of Animal Biology

Azadeh  Kakoie ¹ (Department of Biology, Payame Noor University, Esfahan, Iran)
Akbar  karimi ² (Department of Biology, Payame Noor University, Esfahan, Iran)
shima  toghiani ³ (Department of Biology, Payame Noor University, Esfahan, Iran)

Received: 2019-06-10 Accepted : 2019-12-05 Published : 2019-11-22

Keywords: Rat, Wound healing, Quercus persica, collagen synthesis,

Abstract :

Due to their lower complications than chemical compounds, herbal medicines have received attention from doctors. In this study, Internal Layer of  Persian Oak (Quercus persica), which is used in traditional medicine for chronic dermatologic disorders and eczema, was utilized to investigate cutaneous ulcer healing. Thirty adult male Wistar rats in 5 groups were treated while kept under normal laboratory conditions by creating a wound with dimensions of 1.5 × 1.5 cm2 and a depth of 2 mm in the back. The control group, the sham group, and the experimental groups consist of hydro-alcoholic extract of Internal Layer of Quercus persica at a dose of 250 mg / kg, hydro-alcoholic extract of Quercus persica at a dose of 500 mg / kg, and hydro-alcoholic extract of Quercus persica extract at a dose of 1000 mg / kg, respectively. Topical treatment of ulcers was performed in a 10-day interval every other day, and on the 6th, 8th and 10th days, tissue samples were prepared from the scars. After samples were washed, they were fixed in 10% formalin and hematoxylin-eosin staining of samples, epidermal thickness, wound healing percent, and collagen density were assessed and data were analyzed using SPSS software. In the experimental groups, there was a significant increase (at the level of p < 0.05 and p < 0.01) in the epidermal thickness and the wound healing percent, indicating the significant effect of Internal Layer of Iranian oak Jaft extract on the wound reconstruction, but no significant effect was observed on collagenization in this study Internal Layer of Iranian oak hydroalcoholic extract may have an effective role in accelerating skin wounds healing.

References:

1. Azizi S.H., Ghasemi Pirbalouti, A., Amirmohammadi, M. 2014. Effect of Hydro – alcoholic extract of persian oak (Quercus brantii) in experimentally gastric ulcer. Iranian Journal of Parmaceutieal Research, 13(3): 967-974.

2. Bajalan I. 2013. Identification of Gentisic acid in cupules of oak (Quercus brantii var. persica). Journal of Novel Applied Sciences, 2(8): 253-255.

3. Bajalan I. 2013. Leaves of oak (Quercus branti var. persica) as a rich source of phenolic compounds. International Journal of Farming and Allied Sciences, 2(19): 734-736.

4. Bajalan I. Javadian, M. Zarinkoob, S. Dalvand, H. 2014. Antibacterial Activity of the Extract of Oak (Quercus persica) Fruits. Bulletin of Environment, Pharmacology and Life Sciences, 3(5): 62-65.

5. Belghith S.I., Abidi H., Trabelsi-Ayadi, M., Cherif, J.K. 2015. Study of physicochemicals characteristics and antioxidant capacity of cork oak acorns (Quercus suber L.) grown in three regions in Tunisia. Journal of Applied Pharmaceutical Science, 5(04): 026-032.

6. 12. Ebrahimi A., Khayami M., Nejati V., 2011. Evaluation of the antibacterial and wound healing activity of Quercus persica. International Research Journal of Applied and Basic sciences, 2(8): 328-333.

7. Ghaderi Ghahfarokhi M., Alami M, Sadeghi Mahoonak A.R., Azizi M.H., Ghorbani M. 2012. Effects of phenolic compounds extraction from acorn fruit´s (Quercus branti var persica Lindl.) with different solvents on antioxidant activity in oxidative stability of sunflower oil. Iranian Journal of Medicinal and Aromatic Plants, 28(1): 59-72.

8. Ghorbani Ranjbary A.,  Varzandian S., Zarei A.,  Asmarian S.H.,  Jouibar F. 2015. Investigation of Hydralcoholic Extract of Silybum Marianum on Open Wound Healing in Mice. Journal of Babol University of Medical Sciences, 16(5): 35-41.

9. Heydari M. Poorbabaei, H. Rostami, T. Begim Faghir, M, 2013. Plant species in oak (Quercus brantii Lindl.) understory and their relationship with physical and chemical properties of soil in different altitude classes in the Arghavan valley protected area, Iran. Caspian Journal of Environmental Sciences, 11(1): 97-110. 

10. Jafary A.A.,  Latifi, M., Shohrati A.M.,  Haji Hosseini R.,  Salesi M. 2014. The Effect of Scrophularia striata Extracts on Wound Healing of Mice. Armaghane Danesh Journal of Yasuj University of Medical Sciences, 18(3): 194-209.

11. Karimi A. 2014. Effects of Tragopogon graminifolius on skin wound healing in lewis rats. Journal of Ilam University of Medical Sciences, 22(5) :160-167.

12. Li K., Diao Y., Zhang H., Wang S.H. 2011. Tanin extracts from immature fruits of Terminalia chebula Fructus Retz. promote cutaneous wound healing in rats. BMC Complementary and Alternative Medicine, 11:86-89.

13. Moti F., Heydari Nasrabadi M., Shiravi A., Zahedi F. 2012.The effect of ethanolic extract of Quercus persica on gastric ulcer healing in rats. Journal of Animal Biology, 6(3): 71-80.

14. Natarajan V., Krithica N., Madhan B., Sehgal P.K. 2012. preparation and properties of tannic acid cross- linked collagen scaffold and its application in wound healing. Journal of Biomedical Material Research Part B, 101: 560-567.

15. Nazari  H., Jahanjo N.,  Safarieh M., Taherian M., Khaleghian A., Vafaei  A., Bandegi  A.,  Amrollahi  H., Mohamadi  A. 2011.  The Effect of Avena sativa Alcoholic and Aqueous Extract on the Wound Healing Aand Skin Inflamation. Journal of Urmia University of Medical Sciences, 22(5): 467-473.

16. Nikrooze L, Jafari Barmak M, Naghmachi M, Ghafarian shirazi H, Dehghani N. 2013. Study of Jaft Aqueous Extract and silver sulfadiazine on burn healing in male rat. Armaghane Danesh Journal of Yasuj University of Medical Sciences, 18(2) :107-114.

17. Patil, SM. Saini, R. (2012). Investigation of wound healing activity of Ethyl Acetate extract of flowers of calotropis gigantean. International Journal of Pharmaceutical, Chemical and Biological Sciences, 2(2): 134-137.

18. Sedaghat R., Ghosian Moghadam M.H., Naseri M., Davati A. 2011. Histological evaluation of the anti-inflammatory effects of Alkanna tinctoria on the cutaneous wounds healing in rat. Journal of Hormozgan University of Medical Sciences, 14(4): 281-289.

19. Shiravi, A., Alebouyeh M., Hojati V., Akbari H. 2011. The effect of extract of henna leaves (Lawsonia inermis) on skin wound healing in wistar rats. Journal of Animal Biology, 3(4): 45-51.