Investing effect of Malva , Thymus , Viola and Pinus, Extracts on Candida Glabrata in the In vitro and Animal model.

Subject Areas :

Seyedeh maryam  Moosavinejad ¹ (Department of Microbiology , Faculty of Basic and medical Sciences, Zanjan Branch, Islamic Azad University, Zanjan, Iran)
shahrzad  Nasiri Semnani ² (Department of Microbiology , Faculty of Basic and medical Sciences, Zanjan Branch, Islamic Azad University, Zanjan, Iran.)
Rasol  Shikri ³ (Department of Microbiology , Faculty of Basic and medical Sciences, Zanjan Branch, Islamic Azad University, Zanjan, Iran.)
Reza  Shapouri ⁴ (Department of Microbiology , Faculty of Basic and medical Sciences, Zanjan Branch, Islamic Azad University, Zanjan, Iran.)

Received: 2019-09-23 Accepted : 2019-10-22 Published : 2019-11-22

Keywords:

Abstract :

Inroduction & Objective:The skin infected wound and wounds caused by burn are very epidemic in society, and these kind wounds rate treatment in effective to alleviate the patint, applying the herbal drugs with less complications to treat the wounds. Considering this significance , surveying Investing effect of Malva , Thymus , Viola and Pinus, Extracts on Candida Glabrata in the In vitro and Animal model. Material and Method:In this study, hydroalcholic extracts of Malva , Thymus , Viola and Pinus were prepared in the laboratory. Then, the MIC and MLC of the extract were determined by dilution method in the broth. In the study of the animal model, the bacteria were first inoculated with a concentration of (5×106CFU/ML) to the wound site on Syrian mice. After 24 hours, an ointment prepared with MLC concentration was prepared from extracts was used to treat burns and infections withCandida Glabrata. In the animal model, the extracts of have antimicrobial activity against growth of Candida Glabrata. Results: In this study, The MIC and MLC of the extracts model, the microbia were first inoculated with a concentration of (5×106CFU/ML) to the wound site on Syrian mice. After 24 hours, an ointment prepared with MBC concentration was prepared from extracts of the nettle, watercress and burdock root for 1 g of silver sulfadiazine and was used to treat burns and infections with Candida Glabrata. In the animal model, the extracts antimicrobial activity against growth of Candida Glabrata.In this case, the extract of Pinus and Viola have growth inhibitory Candida Glabrata activity stronger than Malva , Thymus extract. Conclusion: According to the findings of this study, it can be concluded that the extract of these 4 plants on an animal model has antimicrobial and healing effects on Candida Glabrata. It can be used as an anti-inflammatory agent orointment microbia to be raised.

References:

-اکبری، سپیده. 1385. بررسی اثرات ضد قارچی عصاره­های گیاهی آویشن(Thymus vulgaris L.) و مرزنجوش (Origanum vulgare L.) علیه ایزوله های بالینی کاندیدا آلبیکنس مقاوم و حساس به فلوکونازول، فصلنامه گیاهان دارویی، صص 53-62.

2-زارعی، م.، مدنی، ا. 1397. تاثیر عصاره اتانولی گیاه توکریوم پولیوم بر کلونیزاسیون کاندیدا گلابراتا در کبد و طحال و کلیه ها. مجله علمی-پژوهشی دانشگاه علوم پزشکی سبزوار، 25(1): 151-165.

3-میرآبادی، آ.، قمی، د. 1397. ارزیابی اثرات ضد قارچی اسانس روغنی گیاه سیر (Allium sstadium) بر کاندیدا آلبیکنس، کاندیدا گلابراتا و کاندیدا تروپیکالیس در شرایط آزمایشگاهی. فصلنامه بیولوژی کاربردی، 7(28) 11-19.

4.Bineshian, F.(2015). Identification of Candida species using MP65 gene and evaluation of the Candida albicans MP65 gene expression in BALB/C Mice. Jundishapur journal of microbiology, 8(5);134-141.

5.Bergner P. (2001).Treatment garlic. London, UK: Thorsons; 2001.36-45.

6.Choi.J,Kim.W., P. J.y.,Chrong.H.S. (2016). The beneficial effects of Pinus densiflora needls on skin health. Biotechnol.Lett, 44(2); 208-217.

7.Craik, D.J. (1999). Plant cyclotides: a unique family of cyclic and knotted proteins that defines the cyclic cystine knot structural motif. Journal of molecular biology, 294(5);1336-1327.

 

8.Darwish, RM., Aburgia, TA. (2011). Antimicrobial activity of some medicinal plants against different candida species. Jordan Journal of Pharmaceutical Science. 1; 70-80.

9.Dauqan, E.MA., Thamer, F.h., Naji, Kh. M., Alshaibi, Y. (2017). Different extraction methods and antioxidant properties of thyme(Thymus vulganis L.) herb. International journal of chemical Science, 1(2);110-116.

10.Dousti, B. (2018). Comparison of the antifungal effects of various extracts of Onosma chlorotricum on Candida albicans and Candida glaberata with two antibiotics fluconazole and nystatin. Yafte, 20(1); 54-62.

11.Dhuley, J.N. (1999). This article has been retracted Hamycin treatment ofcandidiasis in normal and diabetic rats. FEMS Immunology & Medical Microbiology, 26(2); 175-180.

12.Edmondson, M. (2011). Uncontrolled, open-label, pilot study of tea tree (Melaleuca alternifolia) oil solution in the decolonisation of MRSA positive wounds and its influence on wound healing. Biotechnol.Lett., 24(3);142-151.

13.Fraser, V.J. (1992). Candidemia in a tertiary care hospital: epidemiology, risk factors, and predictors of mortality. Clinical Infectious Diseases, 15(3); 414-421.

14.Hajyani, S., Modaresi, M., Madani, M. (2015). Effect of Malva sylvestris L. extract on blood cell parameters in mice with Candida albicans infection. Der Pharma Chemica, 7(5); 302-305.

15.Ghalem, B. R., Mohamed, B. (2009). Essential oil from gum of Pistacia atlantica Desf. screening of antimicrobial activity. African Journal of Pharmacy and Pharmacology, 3(3); 087-091.

16.Gautam, S.S., Kumar,S. (2012). The antibacterial and phytochemical aspects of Viola odorata Linn. extracts against respiratory tract pathogens. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 82(4); 567-572.

17.Gustafsson, E. (2009). Mathematical modelling of the regulation of spa (protein A) transcription in Staphylococcus aureus. International Journal of Medical Microbiology, 299(1); 65-74.

18.Karaman, S., Digrak, M. (2001). Antibacterial and antifungal activity of the essential oils of tymus revolutus celak from turkey. J.Ehnopharamacology, 76; 183-186.

19.Kovalik. A.O., Biselto, P. (2014). Effects of an orabase for mulation with ethanolic exctra of Malva sylvestris L. in oral wound healing in rats. Journal of Medicinal Food, 1-4.

20.Lindholm, P. (2002). Cyclotides: a novel type of cytotoxic agents 1 PL and UG contributed equally to this manuscript. Molecular cancer therapeutics, 1(6); 365-369.

21.Marotti, M., Piccaglia, R., Giovanelli, E. (1994). Effects of planting time and mineral fertilization on Pep permint essential oil composition and its biological activity. Flovour and Fregrance Journal. 9(3); 125-129.

22.Mustafa, A., Ali, M. (2011). New steroidal lactones and homo mono terpenic glucoside from fruits of Malva sylvestris L. J Acta Pol Pharm, 68(3); 393-401.

23.Mousavi, S.A. (2007). Rapid molecular diagnosis for-candida species using PCR-RFLP. Biotechnology, 6(4); 583-587.

24.Nikzad, H., Atlasi, M. A., Naseri Esfahani, A.H., Naderian, H., Nikzad, M. (2010). Effect of Arnebia leaf on the healing process of rat's second degree burn Feyz. Journal of Kashan University of Medical Sciences, 14(2); 99-106.

25.Panah, H., Bigdeli, M.R., Esmaili, M.A. (2018). Inhibitory effect of Viola odorata exteract on tumor growth and metastasis in 4T1 breast cancer model. IJPR., 17(1); 276-291.

26.Roberts, I., F. Saunders., Boulnois, G. (1989). Bacterial capsules and interactions with complement and phagocytes, Portland Press Limited.

27.Sawhney, AN., Khan, MR., Ndalio, G., Nkunya, M.H.H., Wevers, H. (1987). Studies on the rationale of African traditional medicine. Pak. J. Sci. Ind. Res., 21(5-6); 193-6.

28.Seneviratne, C.J. (2010). Proteomics of drug resistance in Candida glabrata biofilms. Proteomics, 10(7); 1444-1454.

29.Shoham, S., Levitz, S.M. (2005). The immune response to fungal infections. British journal of haematology, 129(5); 569-582.

30.Stuyt, R.J. (2004). Recombinant interleukin-18 protects against disseminated Candida albicans infection in mice. The Journal of infectious diseases, 189(8); 1524-1527.

31.Taheri, J.B. (2018). Study of aqueous and alcoholic extract of the Melissa officinalis effecton Candida albicans, Candida glabrata and Candida krusei. Journal Mil Med, 19(5); 505-512.

32.Vacheva-Dobrevski, R., Kovachev, S., Nacheva, A., Stoev, S., Vasilev, N. (2003). Comparative study of itraconazole and fluconazole therapy in vaginal candidosis. Akush Ginekol, 43(1); 20-3.

33.Tohidi, M., Khayami, M., Nejati, V., Meftahizade, H. (2011). Evaluation of antibacterial activity and wound healing of Pistacia atlantica and Pistacia khinjuk. Journal of Medicinal Plants Research, 5(17); 4310-4314.