Investing effect of Malva , Thymus , Viola and Pinus, Extracts on Candida Glabrata in the In vitro and Animal model.

Subject Areas :

Seyedeh maryam Moosavinejad ¹ , shahrzad Nasiri Semnani ² , Rasol Shikri ³ , Reza Shapouri ⁴

1 - Department of Microbiology , Faculty of Basic and medical Sciences, Zanjan Branch, Islamic Azad University, Zanjan, Iran
2 - Department of Microbiology , Faculty of Basic and medical Sciences, Zanjan Branch, Islamic Azad University, Zanjan, Iran.
3 - Department of Microbiology , Faculty of Basic and medical Sciences, Zanjan Branch, Islamic Azad University, Zanjan, Iran.
4 - Department of Microbiology , Faculty of Basic and medical Sciences, Zanjan Branch, Islamic Azad University, Zanjan, Iran.

Received: 2019-09-23 Accepted : 2019-10-22 Published : 2019-11-22

Keywords:

Abstract :

Inroduction & Objective:The skin infected wound and wounds caused by burn are very epidemic in society, and these kind wounds rate treatment in effective to alleviate the patint, applying the herbal drugs with less complications to treat the wounds. Considering this significance , surveying Investing effect of Malva , Thymus , Viola and Pinus, Extracts on Candida Glabrata in the In vitro and Animal model. Material and Method:In this study, hydroalcholic extracts of Malva , Thymus , Viola and Pinus were prepared in the laboratory. Then, the MIC and MLC of the extract were determined by dilution method in the broth. In the study of the animal model, the bacteria were first inoculated with a concentration of (5×106CFU/ML) to the wound site on Syrian mice. After 24 hours, an ointment prepared with MLC concentration was prepared from extracts was used to treat burns and infections withCandida Glabrata. In the animal model, the extracts of have antimicrobial activity against growth of Candida Glabrata. Results: In this study, The MIC and MLC of the extracts model, the microbia were first inoculated with a concentration of (5×106CFU/ML) to the wound site on Syrian mice. After 24 hours, an ointment prepared with MBC concentration was prepared from extracts of the nettle, watercress and burdock root for 1 g of silver sulfadiazine and was used to treat burns and infections with Candida Glabrata. In the animal model, the extracts antimicrobial activity against growth of Candida Glabrata.In this case, the extract of Pinus and Viola have growth inhibitory Candida Glabrata activity stronger than Malva , Thymus extract. Conclusion: According to the findings of this study, it can be concluded that the extract of these 4 plants on an animal model has antimicrobial and healing effects on Candida Glabrata. It can be used as an anti-inflammatory agent orointment microbia to be raised.

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