Therapeutic effect of cold plasma on burnt skin in adult mice

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Shadi Dorehlo ¹ , simin mohamadi Gorji ² , Nasim Hayati Rodbari ³

1 - Biology Depaetment, Islamic Azad University Science and Research Branch, Tehran, Iran
2 - Biology Depaetment, Islamic Azad University of Sari, Sari, Iran.
3 - Biology Department, Islamic Azad University Science and Research Branch, Tehran, Iran

Received: 2019-10-05 Accepted : 2020-12-24 Published : 2020-08-22

Keywords: Wound healing, Cold plasma, Burned Skin,

Abstract :

Burn wounds significantly heal the patient and these wounds are prone to infection with electrical energy and scar formation. The use of CO2 laser or nitrogen plasma along with the old methods of sterilization and whisper dressing to accelerate the process of blood clotting and wound healing. It may repair the natural process and be more effective than other treatments. Therefore, the aim of this study was to evaluate the effect of cold plasma on heat burn wound on the skin of adult Syrian mice in vivo. 24 adult male mice were randomly divided into control, fuel control, sham and experimental groups. After anesthesia and under sterile conditions, a circular wound with a diameter of 8 mm was created on the back of the mice's neck through hot body contact and caused burns as a zero day. Mice in experimental groups 1 to 3 were then treated daily for 30, 60 and 120 seconds, respectively, by cold or plasma irradiation of helium gas for one week. At the end of the treatment period, the sample bed was tested. The samples were tagged in Bowen and after molding with paraffin, 5 micron thick sections were prepared and stained with hematoxylin and stained. Wound healing is achieved by examining parameters such as the thickness of the stratum corneum, epidermis, dermis, hypodermis, the number of hair follicles and the diameter and number of blood vessels in the skin. Macroscopic examination of the wound site showed that the team of mice with cold plasma reduced the diameter of the burned area. Also, the thickness of skin layers (horny layers, epidermis, dermis and hypodermis) in experimental groups increased significantly (P <0.001) compared to the control group of fuel, as well as the number (P <0.001) and diameter of vessels. Dermal (P <0.01) was also increased compared to the control group of the fuel group. Wound area also decreased significantly (p <0.001) compared to the control group of fuel on the seventh day. The results showed that cold plasma irradiation of helium gas is effective in all performance indicators and by inducing it during burns can help accelerate wound healing.