Evaluation of the combined effect of St John's wort hydroethanolic flower extract and flaxseed oil on skin wound healing -in rats-
Subject Areas :
Veterinary Clinical Pathology
mohammadreza farahpour
1
1 - دانشگاه آزاد اسلامی، واحد ارومیه، استادیار گروه علوم درمانگاهی، دانشکده دامپزشکی، ارومیه، ایران.
Received: 2014-06-06
Accepted : 2014-08-27
Published : 2014-05-22
Keywords:
Rat,
Wound healing,
Flaxseed oil,
<i>St John’s wort</i>,
Ointment,
Abstract :
The use of biological substances is among the methods utilized to increase the rate and quality of wound healing. This experiment was designed to investigate the effects of co-administration of St John's wort hydroethanolic extract and flaxseed oil for acceleration of wound healing based on their therapeutic properties. The study was conducted on 72 male Wistar rats. Experimental wounds measuring 7 mm in diameter was created on both sides of animals then the animals treated with ointments once a day for 2 consecutive weeks. Wound area was determined on days 2, 4, 6, 8, 10, 12 and 14 using a transparent paper. Tissue samples were collected on days 3, 7 and 14 for histopathological studies. According to the data, hydroethanolic extract of St John's wort and flaxseed oil ointment decreased wound area and distribution of polymorphonuclear immune cells in wound area as well as increase in new vessel formation and fibroplasia (P<0.05). Furthermore, wound healing process was better in group treated with hydroethanolic extract of St John's wort and flaxseed oil ointment compared to the other groups.
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