Study of the effect of supplement of soy isoflavone on the healing process of cutaneous wound in ovariectomized cats

Subject Areas : Journal of Comparative Pathobiology

, M  Gholami ¹ (Graduated from faculty of veterinary medicine, semnan university, semnan, Iran)
, HR  Moslemi ² (Department of clinical sciences, faculty of veterinary medicine, semnan university, semnan, Iran)
, A  Javaheri Vayeghan ³ (Department of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran)
رضا  Narenji Sani, ⁴ (Department of clinical sciences, faculty of veterinary medicine, semnan university, semnan, Iran)

Received: 2019-06-10 Accepted : 2019-06-10 Published : 2019-05-22

Keywords: Soy isoflavone, Ovariectomy, Wound, Cat,

Abstract :

Sterilization surgery in dogs and cats is a common surgical procedure in veterinary medicine, which can be done in two ways: Ovariohysterectomy (OHE) and Ovariectomy (OVE). Since some diets in cats contain lots of Genistein and Daidzein, studying of the effect of soy isoflavone on the wound healing process is essential. In this study, 10 cats of the DSH, 1 year old, were used. OVE was done by midline approach. After surgery, cats divided into two groups including isoflavone and placebo. The isoflavone group received 50 mg of oral isoflavone daily. In the control group, placebo was used. After 14 and 28 days, biopsy of the surgical wound was taken for histopathological examination to evaluate the amount of collagen, angiogenesis and fibroblasts. Based on the findings, On Day 14 after injury, wound micrographs showed that there was no significant difference between treatment and control groups (p < /em> ≥ 0.05). Furthermore, on day 28 after the injury, the healing rate in the treatment group was higher than control group significantly (P≤0.05). In summary, results of this study showed that the application of supplement of soy isoflavone could improve the healing process of cutaneous wound in ovariectomized cats.

References:

1. Ashcroft, G.S., Mills, S.J., Lei, K., Gibbons, L., Jeong, M.J., Taniguchi, M., Burow, M., Horan, M.A., Wahl, S.M., Nakayama, T.(2003): Estrogen modulates cutaneous wound healing by downregulating macrophage migration inhibitory factor. J. Clin. Invest. 111(9): 1309-1318.


2. Ashcroft, G.S. (2004): Sex differences in wound healing. Adv. Mol. Cell. Biol. 34: 321-328.


3. Brownson, D.M., Azios, N.G., Fuqua, B.K., Dharmawardhane, S.F., Mabry, T.J. (2002): Flavonoid effects relevant to cancer. J. Nutr. 132: 3482-3489.


4. Darby, I.A., Laverdet, B., Bonté, F., Desmoulière, A. (2014): Fibroblasts and myofibroblasts in wound healing. Clin. Cosmet.Investig.Dermato. 7: 301-311.


5. Gal, P., Kilik, R., Mokry, M., Vidinsky, B., Vasilenko, T., Mozes, S., Lenhardt, L. (2008): Simple method of open skin wound healing model in corticosteroid-treated and diabetic rats: standardization of semi-quantitative and quantitative histological assessments. Vet. Med. 53(12): 652-965.


6. Gower, S., Mayhew, P. (2008): Canine laparoscopic and laparoscopic-assisted ovarriohysterectomy and ovariectomy. Compendium (Yardley, PA). 30: 430-440.


7. Guo, S.A., DiPietro, L.A. (2010): Factors affecting wound healing. J.Dent.Res. 89(3): 219-229.


8. Henderson, J.L., Cupp, C.L., Ross, E.V., Shick, P.C. (2003): The effects of autologous platelet gel on wound healing. Ear.Nose.Throat.J. 82(8): 598-602.


9. Holocomb, V.B., Keck, V.A., Barrett, J.C., Hong, J., Libutti, S.K., Nunez, N.P. (2009): Obesity Impairs Wound Healing in Ovariectomized Female Mice. In Vivo. 23(4): 515-518.


10. Horng, H.C., Chang, W.H., Yeh, C.C., Huang, B.S., Wang, P.H., Chang, C.P., Chen, Y.J., Tsui, K.H. (2017):Estrogen Effects on Wound Healing. Int. J. Mol. Sci. 18(2325): 1-14.


11. Johnson, K.E., Wilgus, T.A. (2014): Vascular endothelial growth factor and angiogenesis in the regulation of cutaneous wound repair. Adv. Wound Care. 3(10): 647-661.


12. Leen, L.L., Filip, C., Billon, A., Garmy-Susini, B., Jalvy, S., Robbesyn, F., Daret, D., Allieres, C., Rittling, S.R., Werner, N., Nickenig, G.,  U., Duplaa, C., Dufourcq, P., Lenfant, F., Desgranges, C., Arnal, J.F., Gadeau, A.P. (2008): Estrogen-Stimulated Endothelial Repair Requires Osteopntin. Arterioscler. Thromb. Vasc. Biol. 28(12): 2131-2136.

 

13. Morito, K., Hirose,T., Kinjo,J., Hirakawa, T.,Okawa, M.,Nohara,T.,Ogawa, S., Inoue, S., Muramatsu, M., Masamune, Y.(2001): Interaction of phytoestrogens with estrogen receptors alpha andbeta. Biol. Pharm. Bull. 24(4): 351–356.


14. Rajabi, M.A., Rajabi, F. (2007): The effect of estrogen on wound healing in Rats. Pak. J. Med. Sci. 23(3): 394-352.


15. Routley, C.E., Ashcroft, G.S. (2009): Effect of estrogen and progesterone on macrophage activation during wound healing. Wound Repair Regen. 17(1): 42-50.


16. Song, T.T., Hendrich, S., Murphy, P.A. (1999): Estrogenic activity of glycitein, a soy isoflavone. J.Agric. Food. Chem.47(4): 1607– 1610.


17. Stevenson, S., Taylor, A.H., Meskiri, A., Sharpe, D.T., Thornton, M.J. (2008): Differing responses of human follicular and nonfollicular scalp cells in an in vitro wound healing. Wound.Rep. Reg. 16: 243-253.


18. Suntar, I., Tatlı, I.I., Akkol, E.K., Keleş, H., Kahraman, C., Akdemir, Z. (2010): An ethnopharmacological study on Verbascum species: From conventional wound healing use to scientific verification. J.Ethnopharmacol. 132(2): 408-413.


19. Wilkinson, H.N., Hardman, M.J. (2017): The role of estrogen in cutaneous ageing and repair.103: 60-64.


20. Zhou, T., Yang, Z., Chen, Y., Chen, Y., Huang, Z., You, B., Peng, Y., Chen, J. (2016): Estrogen Accelerates Cutaneous Wound Healing by Promoting Proliferation of Epidermal Keratinocytes via Erk/Akt Signaling Pathway. Physiol. Biochem.38(3): 959-968.