A comparative study of the effects
of hydroalcoholic extracts of purslane (Portulaca oleracea L.) and henna leaves (Lawsonia inermis L.) on wound healing
in male Wistar rats
Subject Areas :
zoology
Fatemeh
Jamallou
1
(Assistant Professor, Department of Biology, Qom Branch, Islsmic Azad University, Qom, Iran)
Received: 2022-06-25
Accepted : 2022-09-02
Published : 2022-09-23
Keywords:
Ointment,
Purslane plant,
Male wistar rats,
Skin wound,
Henna plant,
Hydroalcoholic extracts,
Abstract :
Objectives: One of the medicinal plants known in traditional medicine, henna with the scientific name Lawsonia inermis L. comes from the Lythraceae family and purslane with the scientific name Portulaca oleracea L. comes from the Portulacaceae family. According to the compounds present in these plants, many properties such as antimicrobial, anti-inflammatory, wound healing, trypsin inhibitory and antioxidant properties have been reported for them.Materials and Methods: This study was conducted on 24 male rats in the weight range of 200±20 grams, which were divided into 4 groups of six. Wounds 2 cm long were created on the back skin of mice: the first group was kept without treatment as a control, the second group was treated with Esrin (negative control) and the other two groups were treated with ointments made of henna and purslane with a concentration of 50% by weight. -Weight was used in the daily basis of Esrin. The area of the wound was measured daily from the second to the fourteenth day with Autocade software. The data were analyzed using SPSS software at a significance level of p<0.05.Findings: The decrease in wound area was significantly higher in all groups treated with purslane and henna. Purslane showed a better effect.Conclusion: Henna and purslane ointment can be used as effective substances in the treatment of skin wounds by significantly reducing the healing time and wound area.
References:
Ajand N & Roshanaei K. Investigating the properties of plant root extract Withania somnifera In the treatment of skin wounds of male Wistar rats. Journal of Shahid Sadoughi University of Medical Sciences, Yazd. 2014; 23(9): 900-911.
Afshar M, Satari Fard H, Shadi M & Qadri R. Restorative effects of medicinal plants native to Iran on the healing of wounds caused by cuts. Journal of Birjand University of Medical Sciences. 2013; 22(1): 1-18.
Afshar M, Vafainejad S, Shadi M & Qadri R. Medicinal plants effective in healing burn wounds in Iran's herbal flora. Journal of Gorgan University of Medical Sciences. 2016; 19(3): 1-14.
Emami Y, Shams Ardakani MR & Nekoui Naini N. Herbal medicine, treatment of diseases by plants. Tehran: Green Arang Publications; 2013: 187-189
Barati B, Abbasi H. A comparative study of the effectiveness of tetracycline and topical hydrogen peroxide solution in healing facial wounds. Journal of Qom University of Medical Sciences. 2007; 1(2): 59-53.
Hashemlovian Delnawaz B & Atai Azimi A. Medicinal and edible properties of plants with emphasis on simple plants. Publications of Islamic Azad University, Saveh Branch; 2007: 61-62.
Rezaei A, Pighan R, Tulabi Dezfuli Z & Iftikhar Manavi, Sh. Macroscopic and microscopic examination of alcoholic extract of Lawsonia inermis in the healing of experimental skin damage created in the skin of macro fish (Labidochromis caeruleus). Fisheries of Iran. 2013; 23(2): 45-56.
Zargari A. Medicinal plants (Vol. 1, 8th ). Tehran: University Publications; 2016:
324-322.
Zargari A. Medicinal plants (Vol. 2). Tehran: University Publications; 1996: 363-353
Simpson MJ. Plant systematics (Vol. 1). Translated by F Garhannijad, P Poya & A Hosseini. House of Biology Publications; 2012: 383-312.
Siravi A, Al Boyeh M, Hojjati V & Akbari H. Investigating the effect of Lawsonia inermis leaves on skin wound healing in male Wistar rats. Animal biology. 2019; 3(4): 45-52.
Shahsawari A. Plant systematics, origin and classification of plants. Bu Ali Sina University Press, 2013: 435-431.
Fatahi Bafghi A, Fallahzadeh H & Mossadegh M. Investigating the effectiveness of lawsonia inermis extract on salicy wound in BALB/c mice. Journal of Kerman University of Medical Sciences. 2017; 4: 329-330.
Ghahreman A. Cormophytes of Iran (plant systematics) (Vol.2). Tehran: University Publishing Center Publications, 1998: 621-625.
Ghahreman A. Cormophytes of Iran (plant systematics). (Vol.1). Tehran: University Publishing Center Publications, 1998: 674-673.
Majd A & Sharif Shushtri M. Examining the anatomical structure, karyological characteristics, stages of pollen development and their allergenic ability in Marguerite plant. Master's thesis. North Tehran Branch, Islamic Azad University, 2018: 6-38.
Mozaffarian V. Plant classification: Dicotyledonous. Tehran: Amirkabir Publications, 2014: 488-495.
Mir Owliyayi M, Chelongar R, Amin Jafari A, Talebi A & Ghiyath M. Investigation of histopathology of non-infectious deep skin wound healing with hydroalcoholic extracts of mountain savory, lemon balm, cedar, marshmallow and, Lawsonia inermis plants. Cell and molecular researches (Biology of Iran). 2016; 30(2): 1-10.
Jafarnia S & Sara Khosroshahi M. A comprehensive and illustrated guide to the properties and uses of medicinal plants. Publication of Sokhan Gostar, 2016: p. 4. [in persian]
Nag Chaudhuri AK & Dilip Roy S.K. Anti-inflammatory activity of Indian black tea (Sikkim Variety). Pharmacological Research. 2005; 51: 169-175.
Asquith HH & Butler N. Purification of Quebracho Tannin. Chem. Ecol. 1985; 11: 1535-1544.
Chaudhary G, Goyal S & Poonia P. Lawsonia inermis A phytopharmacological review. International Journal of Pharmaceutical Science and Drug Research. 2012; 2(2): 91-98.
De Araujo FVS, Coelho de Souza AN, Morais SM, Santos CF & Leal Cardoso JH. Antinociceptive effects of the essential oil of alpinia zerumbet on mice. Phytomedicine. 2005; 12: 482-496.
De Sousa Arajo TA & Alencar NL. A new approach to study medicinal plants with tannins and flavonoids contents from the local knowledge. Journal of Ethnopharmacology. 2008; 120(1): 72-80.
Dholwani KK, Saluja AK, Gupta AR & Shah DR. A review on plant-derived natural products and their analogs with anti-tumor activity. Indian J pharmacol. 2008; 40: 49-58.
Diegelmann RF & Evans MC. Wound Healing: An Overview of Acute, Fibrotic and Delayed Healing. Frontiers in Bioscience. 2004; 9(1): 283-289.
Fallahi F, Roghani M & Bagheri A. Time-Dependent Hypoglycemic and Hypolipidemic Effect of Allium Ascalonicum L. Feeding in Diabetic Rats. Journal of Babol University of Medical Sciences. 2010; 12(1): 16-23.
Fonder MA & et al. Treating the chronic wound: A practical approach to the care of nonhealing wounds and wound care dressings. J Am Acad Dermatal. 2008; 58(2): 185-206.
Kandaswami C & Middleton E. Free radical scavenging and antioxidant activity of plant flavonoids. Advanses in Experimental Medicine and Biology. 1994; 366: 351-376.
Kim HK & Cheon BS. Effects of naturally occurring flavonoids on nitric oxide production in the macrophage cell line RAW and their structure-activity relationships. Biochemical pharmacology. 1999; 58(5): 759-765.
Mahato SB & Kundu A. 13C NMR spectra of pentacyclic triterpenoids - A compilation and some salient features. Phytochemistry. 1994; 37(6): 1517-1575.
Middleton JE & Kandaswami C. Effects of flavonoids on immune and inflammatory cell functions. Biochemical pharmacology. 1992; 43(6): 1167-1175.
Nijveldt RJ, Van nood E, Van Horn D, Boelens PG, Van Norren K & Van Leeuwen P. Flavonoids: a review of probable mechanisms of action and potential applications. The American Journal of Clinical Nutritions. 2001; 74(4): 418-425.
Parejo I, Viladomat F, Bastida J, Rosas-Romero A, Flerlage N, Burillo J & Codina C. Comparison between the radical scavenging activity and antioxidant activity of six distilled and nondistilled Mediterranean herbs and aromatic plants. Agric. Food Chem. 2002; 50: 6882-6890.
Raina R & Prawez S. Medicinal Plants and their Role in Wound Healing. Vet Scan. 2008; 3(1): 1-7.
Rajwar S & Khatri P. Pharmacognostic and phytochemical studies on various plant parts of Lawsonia inermis Asian Journal of Pharmaceutical Sciences and Clinical Research. 2011; 1: 22-40.
Reinhard G & Matthias G. Chemokines in cutaneous wound healing. Journal of Leukocyte Biology. 2001; 69: 513-521.
Sharifi R, Pasalar P, Kamalinejad M, Dehpour AR & Tavangar SM. The effect of silymarin (Silybum marianum) on human skin fibroblasts in an in vitro wound healing model. Pharm Biol. 2013; 51(3): 298-303.
Souba WW & Wilmore D. Diet and nutrition in case of the patient with surgery. 9th Baltimore: Williams & Wilkins Press, 1999: 1589-1618.
Vasconcelos PCP & Andreo MA. Effect of Mouriri pusa tannins and flavonoids on prevention and treatment against experimental gastric ulcer. Journal of Ethnopharmacology. 2010; 131(1): 146-153.
_||_
Ajand N & Roshanaei K. Investigating the properties of plant root extract Withania somnifera In the treatment of skin wounds of male Wistar rats. Journal of Shahid Sadoughi University of Medical Sciences, Yazd. 2014; 23(9): 900-911.
Afshar M, Satari Fard H, Shadi M & Qadri R. Restorative effects of medicinal plants native to Iran on the healing of wounds caused by cuts. Journal of Birjand University of Medical Sciences. 2013; 22(1): 1-18.
Afshar M, Vafainejad S, Shadi M & Qadri R. Medicinal plants effective in healing burn wounds in Iran's herbal flora. Journal of Gorgan University of Medical Sciences. 2016; 19(3): 1-14.
Emami Y, Shams Ardakani MR & Nekoui Naini N. Herbal medicine, treatment of diseases by plants. Tehran: Green Arang Publications; 2013: 187-189
Barati B, Abbasi H. A comparative study of the effectiveness of tetracycline and topical hydrogen peroxide solution in healing facial wounds. Journal of Qom University of Medical Sciences. 2007; 1(2): 59-53.
Hashemlovian Delnawaz B & Atai Azimi A. Medicinal and edible properties of plants with emphasis on simple plants. Publications of Islamic Azad University, Saveh Branch; 2007: 61-62.
Rezaei A, Pighan R, Tulabi Dezfuli Z & Iftikhar Manavi, Sh. Macroscopic and microscopic examination of alcoholic extract of Lawsonia inermis in the healing of experimental skin damage created in the skin of macro fish (Labidochromis caeruleus). Fisheries of Iran. 2013; 23(2): 45-56.
Zargari A. Medicinal plants (Vol. 1, 8th ). Tehran: University Publications; 2016:
324-322.
Zargari A. Medicinal plants (Vol. 2). Tehran: University Publications; 1996: 363-353
Simpson MJ. Plant systematics (Vol. 1). Translated by F Garhannijad, P Poya & A Hosseini. House of Biology Publications; 2012: 383-312.
Siravi A, Al Boyeh M, Hojjati V & Akbari H. Investigating the effect of Lawsonia inermis leaves on skin wound healing in male Wistar rats. Animal biology. 2019; 3(4): 45-52.
Shahsawari A. Plant systematics, origin and classification of plants. Bu Ali Sina University Press, 2013: 435-431.
Fatahi Bafghi A, Fallahzadeh H & Mossadegh M. Investigating the effectiveness of lawsonia inermis extract on salicy wound in BALB/c mice. Journal of Kerman University of Medical Sciences. 2017; 4: 329-330.
Ghahreman A. Cormophytes of Iran (plant systematics) (Vol.2). Tehran: University Publishing Center Publications, 1998: 621-625.
Ghahreman A. Cormophytes of Iran (plant systematics). (Vol.1). Tehran: University Publishing Center Publications, 1998: 674-673.
Majd A & Sharif Shushtri M. Examining the anatomical structure, karyological characteristics, stages of pollen development and their allergenic ability in Marguerite plant. Master's thesis. North Tehran Branch, Islamic Azad University, 2018: 6-38.
Mozaffarian V. Plant classification: Dicotyledonous. Tehran: Amirkabir Publications, 2014: 488-495.
Mir Owliyayi M, Chelongar R, Amin Jafari A, Talebi A & Ghiyath M. Investigation of histopathology of non-infectious deep skin wound healing with hydroalcoholic extracts of mountain savory, lemon balm, cedar, marshmallow and, Lawsonia inermis plants. Cell and molecular researches (Biology of Iran). 2016; 30(2): 1-10.
Jafarnia S & Sara Khosroshahi M. A comprehensive and illustrated guide to the properties and uses of medicinal plants. Publication of Sokhan Gostar, 2016: p. 4. [in persian]
Nag Chaudhuri AK & Dilip Roy S.K. Anti-inflammatory activity of Indian black tea (Sikkim Variety). Pharmacological Research. 2005; 51: 169-175.
Asquith HH & Butler N. Purification of Quebracho Tannin. Chem. Ecol. 1985; 11: 1535-1544.
Chaudhary G, Goyal S & Poonia P. Lawsonia inermis A phytopharmacological review. International Journal of Pharmaceutical Science and Drug Research. 2012; 2(2): 91-98.
De Araujo FVS, Coelho de Souza AN, Morais SM, Santos CF & Leal Cardoso JH. Antinociceptive effects of the essential oil of alpinia zerumbet on mice. Phytomedicine. 2005; 12: 482-496.
De Sousa Arajo TA & Alencar NL. A new approach to study medicinal plants with tannins and flavonoids contents from the local knowledge. Journal of Ethnopharmacology. 2008; 120(1): 72-80.
Dholwani KK, Saluja AK, Gupta AR & Shah DR. A review on plant-derived natural products and their analogs with anti-tumor activity. Indian J pharmacol. 2008; 40: 49-58.
Diegelmann RF & Evans MC. Wound Healing: An Overview of Acute, Fibrotic and Delayed Healing. Frontiers in Bioscience. 2004; 9(1): 283-289.
Fallahi F, Roghani M & Bagheri A. Time-Dependent Hypoglycemic and Hypolipidemic Effect of Allium Ascalonicum L. Feeding in Diabetic Rats. Journal of Babol University of Medical Sciences. 2010; 12(1): 16-23.
Fonder MA & et al. Treating the chronic wound: A practical approach to the care of nonhealing wounds and wound care dressings. J Am Acad Dermatal. 2008; 58(2): 185-206.
Kandaswami C & Middleton E. Free radical scavenging and antioxidant activity of plant flavonoids. Advanses in Experimental Medicine and Biology. 1994; 366: 351-376.
Kim HK & Cheon BS. Effects of naturally occurring flavonoids on nitric oxide production in the macrophage cell line RAW and their structure-activity relationships. Biochemical pharmacology. 1999; 58(5): 759-765.
Mahato SB & Kundu A. 13C NMR spectra of pentacyclic triterpenoids - A compilation and some salient features. Phytochemistry. 1994; 37(6): 1517-1575.
Middleton JE & Kandaswami C. Effects of flavonoids on immune and inflammatory cell functions. Biochemical pharmacology. 1992; 43(6): 1167-1175.
Nijveldt RJ, Van nood E, Van Horn D, Boelens PG, Van Norren K & Van Leeuwen P. Flavonoids: a review of probable mechanisms of action and potential applications. The American Journal of Clinical Nutritions. 2001; 74(4): 418-425.
Parejo I, Viladomat F, Bastida J, Rosas-Romero A, Flerlage N, Burillo J & Codina C. Comparison between the radical scavenging activity and antioxidant activity of six distilled and nondistilled Mediterranean herbs and aromatic plants. Agric. Food Chem. 2002; 50: 6882-6890.
Raina R & Prawez S. Medicinal Plants and their Role in Wound Healing. Vet Scan. 2008; 3(1): 1-7.
Rajwar S & Khatri P. Pharmacognostic and phytochemical studies on various plant parts of Lawsonia inermis Asian Journal of Pharmaceutical Sciences and Clinical Research. 2011; 1: 22-40.
Reinhard G & Matthias G. Chemokines in cutaneous wound healing. Journal of Leukocyte Biology. 2001; 69: 513-521.
Sharifi R, Pasalar P, Kamalinejad M, Dehpour AR & Tavangar SM. The effect of silymarin (Silybum marianum) on human skin fibroblasts in an in vitro wound healing model. Pharm Biol. 2013; 51(3): 298-303.
Souba WW & Wilmore D. Diet and nutrition in case of the patient with surgery. 9th Baltimore: Williams & Wilkins Press, 1999: 1589-1618.
Vasconcelos PCP & Andreo MA. Effect of Mouriri pusa tannins and flavonoids on prevention and treatment against experimental gastric ulcer. Journal of Ethnopharmacology. 2010; 131(1): 146-153.
