Morphological and histopatological study of Pratylenchus pseudocoffeae on Chrysanthemum in Mahallat region
Subject Areas : New Finding in AgricultureE. Sedaghatfar 1 , A. Mohammad Deimi 2
1 - Islamic Azad University, Arak, Iran
2 - Graduated M. Sc. Student, Islamic Azad University, Science and Research Campus, Tehran, Iran
Keywords: Iran, Morphology, Chrysanthemum, Pratylenchus pseudocoffeae, Root lesion nematode,
Abstract :
In order to identify the plant parasitic nematodes of Chrysanthemum in greenhouses and open fields in Mahallat region located in Markazi province in Iran, about 67 soil and root samples were collected during 2007 and 2008. The nematodes were extracted by centrifugal technique, killed, fixed and transferred to glycerine. Morphological and morphometrical characters were studied by light microscope. In our study, one of the most important nematodes was root-lesion nematode Pratylenchus pseudocoffeae. Root tissues were invaded by the nematode, which caused distinct necrosis and large cavities within cortical parenchyma. All developmental stages were observed in the root tissues. Morphometrics of P. pseudocoffeae is characterised by a labial region with three annuli, vulva located at 79-82% of body, long differentiated post-vulval uterine sac, tail sub hemispherical or bluntly pointed with smooth terminus, presence of males, spermatheca oblong. The species resembles P. coffeae, P. gutierrezi and P. penetrans from which it differs by a number of morphometric features (position of the vulva, the shape of the tail tip and head shape). This is a new occurrence and first record of the species in Iran and becomes the third world record after his original description.
1-حسنیمهربان، ا. و تنهامعافی، ز. 1379. وجود نماتد ریشه گرهی در گلخانههای پرورش میخک محلات خلاصه مقالات چهاردهمین کنگره گیاهپزشکی ایران. صفحه 357.
2- مهدیخانی مقدم، ع.، خیری، ا. و محمدی، م. 1382.معرفی سه گونه جدید از جنس Meloidogyneبرای ایران، مجله بیماریهای گیاهی، جلد 189، صفحه 239ـ211.
3- Castillo, P.and Volvas, N. 2007. Pratylenchus (Nematoda: Pratylenchidae): Diagnosis, Biology, Pathogenicity and Management. Brill Academic Publishers, Leiden, Netherlands, 555 pp.
4- Coolen, W.A. and D’Hered, C. J. 1972. Amethod for the quantitative extraction of nematodes from plant tissue.9220 Merelbek Belgium,77 pp.
5- Corbett, D. C. M. 1973. Prtylenchus penetrans. C.I.H Descriptionsof Plant Parasitic Nematodes Set.2 No 25 Farnham Royal, UK: CAB, 3pp.
6- Corbett, D. C. M. and Clark, S. 1983. Surface structures in the taxonomy of Pratylenchus species. Revue de Nématology 6: 85-98.
7- De Grisse, A. T. 1969. Redescription ou modification de quelques techniques dans L’etude des nematodes phytoparasitaires. Mede. Rijks. Fak. LandbWet Gent, 34: 351-369.
8- Inserra, R. N., Duncan, L. W., Dunn, D., Kaplan, D. T. and Porazinska, D. 1998. Pratylenchus pseudocoffeae from Florida and its relationship with P. gutierrezi and P. coffeae. Nematologica 44: 683-712.
9- Jenkins, W. R. 1964. Arapid centrifugal flotation technique for separating nematodes from soil. PL. Dis. Reptr. 48(9): 692.
10- Johansen, D. A. 1940. Plant Microtechnique. New York, USA: McGraw-Hill, 523 pp.
11-Lee, J. K., Choi, D. R., Park, B. Y. and Kim, D. G. 2006. Occurrence of root lesion nematodes Pratylenchus spp. from chrysanthemum in Korea. Journal ofNematology 38, 279 (abstract).
12- Mizukubo, T. 1992. Pratylenchus pseudocoffeae n.sp. (Nematoda: Pratylenchidae) from compositae plants in Japan. Applied Entomology and Zoology 27: 437-444.
13- Tanha Maafi, Z., Sturhan, D., Kheiri, A., Geraert, E., Subbotin, S. A. and Moens, M. 2003. Morphology of some cyst-forming nematodes from Iran. Russian Journal of Nematology 11: 59-78.
14- Vovlas, N. and Troccoli, A. 1990. Histopathology of broad been roots infected by the lesion nematode Pratylenchus penetrans. Nematologia Mediterranea 18: 239-242.
