Evaluation of antifungal activity of topical Rosmarinus efficinalis essential oil on infected cutaneous wound with Candida albicans in rats: Histopathological study

Subject Areas : Journal of Comparative Pathobiology

Nejati, H., Farahpour, M.R.*, Neiriz Naghadehi, M. . ¹ ( )

Received: 2014-03-16 Accepted : 2014-06-22 Published : 2014-09-23

Keywords: Candida albicans, Rosmarinus Officinalis, Wound, Histopathology, Rat,

Abstract :

Today, due to side effects and development of drug resistance to Azol drugs and its derivatives,which used for treatment of Candida infections in either topical or oral forms, led to the use ofbiological materials. One of medicinal herb have been used more frequently, the past to thepresent, is Rosemary (Rosmarinus officinalis L.). In this study on 45 male Wistar-albino rats(weight 210±10 g), after general anesthesia, and a wound square with dimensions 1/5 in the 1/5cm area between the shoulder, immediately was applied to the wound 0.1 ml of the suspensioncontaining 1/5×107 CFU Candida albicans yeast. Then tested in three groups of 15 rats each(control, topical ointment containing 1.5% and 3% Rosmarinus officinalis oil) were randomlydistributed into 5 subgroups of 3 rats each (sample groups on different days) groups. End ofdays 4th, 8th, 12th, 16th and 20th from wounds of different groups, in order to histopathology andyeast counts by a special punch biopsy specimen. Investigation showed that the use of topical1.5 and 3 percent of Rosmarinus essential oil compared with the control group cause thedecreased substantially in infection rates and increased volume production of collagen and islined. According to the finding results, wound healing is better at 3% Rosmarinus officinalisessential oil ointment treated group, compared to lower treatment dose and control groups.