Histopathological effects of flowering shoot hydroethanol extracts of Hypericumperforatum on experimental full-thickness skin wound healing in rats
Subject Areas : clinical veterinary science, M AsghariehAhary 1 , M.R Farahpour, 2
1 - Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, urmia, Iran
2 - Department of clinical science, Faculty of veterinary medicine, Urmia Branch, Islamic
Azad university, Urmia branch, Urmia, Iran
Keywords: Hypericumperforatum, Hydroethanolextract, ointment, Full-Thickness Skin Incision, Wound, Healing, Rat,
Abstract :
Wound healing has always been of great concern for the surgeon and therefore using different effective biologicalsubstances of either plant or animal origin for a faster and more effcient wound healing (repair) has been considered.The aim of this study was to investigate the accelerating effects of Hypericumperforatumhydroethanolextracton open wound healing in rats. Study design: thirty-six adult male Wistar-albino rats (weight 200±10 g) wererandomly allocated to three groups (one control and two treatment groups). Two seven-mm, circular, fullthicknesses, skin wounds were created on the back of each rat (2 on the left and 2 on the right side). In the controlgroup, wounds were treated by an ointment with no extract. The treatment groups were treated by standardizedointment containing 1% and 2% Hypericumperforatum extract. For histopathological studies, the 3 groups weresubdivided into 4 subgroups with 4 rats in each. Samples were taken from the groups 1 to 4 on days 3, 7, 14, and21, respectively. After taking the sample, the rats were euthanized. The sequential wound biopsies were evaluatedhistopathologically for their heamorrage,neutrophils and lymphocyte, new vascularization and re epithelialization.According to the results of this study, the ointment with 2% extract was much better and slightly better than thecontrol and 1% extract ointments, respectively.
