Histopathological evaluation of the effect of Mentha piperita essential oil on cutaneous wound healing in rats infected with C. albicans

Subject Areas : Journal of Comparative Pathobiology

N. Farhangi Ghaleh Joughi, ¹ , M.R. Farahpour, ² , M. Neiriz Naghadeh, ³

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Received: 2014-01-13 Accepted : 2014-05-06 Published : 2015-01-21

Keywords: Mentha Piperita, Candida albicans, Wound Healing, Rat,

Abstract :

Wound infections caused by Candida albicans has grown substantially in recent years.Expensive medications, side effects, and particularly, the development of drug resistance, led tothe use of biological materials may be considered as an alternative solution. Studies show thatMentha piperita essential oil contains flavonoids and menthol and hence it has antibacterialproperties. We used 100 male Wistar rats (weight 195-205 g). One square surgical wound withdimensions of 1.5×1.5 cm were performed on the back of each animal and immediately becameinfected whit 0.1 ml of 1.5×107 CFU Candida albicans suspension. Then the rats were dividedinto 4 groups (control, placebo, treatments 1.5% and 3%) each with 25 rats and randomlydistributed into 5 subgroups each whit 5 rats (sample groups on different days). Wound healingactivity was performed by histological studies and yeast counts at the end of 4th, 8th, 12th, 16thand 20th days after surgery. According to the pathological findings, Mentha piperita essential oiltopically, significantly reduces inflammation and migration of leukocytes, and also significantlyincrease in vascular regeneration, epithelialization and migration of fibroblasts, comparedcontrol group. Mentha piperita essential oil, especially in higher doses (3%), increasedpathological factors affecting wound healing in rats was infected with Candida albicans.