Collagen Turnover in Healing of Cutaneous Wounds by Aloe vera Fresh Gel in Mouse: Involvement of Fibroblast Growth Factor Receptor
Subject Areas :
Mehran
Arabi
1
(Associate Prof. in Physiology, Department of Animal Sciences, Faculty of Basic Sciences, Shahrekord University, Iran)
homeira
Jafarzadeh
2
(MSc in Physiology, Department of Animal Sciences, Faculty of Basic Sciences, Shahrekord University, Iran.)
Keywords: Aloe vera gel, Wound healing, Growth factor, Mouse Skin, Collagen Turnover,
Abstract :
Inroduction & Objective: Using herbal products like Aloe vera (Aloe barbadensis Miller) plays an important role in wound healing. This study aimed to evalutea the curative influence of Aloe vera (AV) fresh gel on healing of open cutaneous wounds in mouse. Material and Method: Mice were 1) pseudo-control (sham): wound treated with physiological serum; 2) wound treated with 2 gr AV fresh gel. Two equal full-thickness skin wounds were made on vertebral column in the sacral region. On 8th and 16th post wounding day, skin sampling for the purpose of gene expression of fibroblast growth factor receptor (FGF-R) under RT-PCR evaluation was taken place. Urine samples were used to determine the concentration of hydroxy proline (HP) as a marker of collagen turnover in wounds, using HP assay kit. Results: AV fresh gel could up-regulate the expression of FGF-R gene as compared to negative and pseudo-controls (P<0.05). In addition, HP concentration was incresed in AV gel-treated groups comparing to others. Conclusion: We revealed that AV fresh gel was capable of healing wounds via elevation in collagen turnover and represents a good candidate for a wound healing process in human and animals.
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