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      • Open Access Article

        1 - Production of probiotic juice by use of two varieties of Red and Yellow apple
        neda Ghazavi Hamdollah Moshtaghi Mojtaba Bonyadian
        Probiotic bacteria due to having positive effects on health, widely used in the manufacture of foods, particularly in the production of dairy products, but having lactose intolerance problem and high cholesterol of dairy products is an important restrictions on the use More
        Probiotic bacteria due to having positive effects on health, widely used in the manufacture of foods, particularly in the production of dairy products, but having lactose intolerance problem and high cholesterol of dairy products is an important restrictions on the use of them. Recently, the consumption of non-dairy probiotic foods and specifically non-dairy probiotic juices were developed. The aim of this study was to examine the growth trend of Lactobacillus acidophilus in the two types of red and yellow apple. After inoculation of bacteria to each sample, they were stored at 4°C and at certain times during fermentation, changes in pH, titratable acidity and the sediment, as well as live cell counts were measured under controlled conditions. Both treatments showed a significant decrease in pH, sediment and bacteria population at the end of maintenance, but the changes in acidity were not significant (p<0.05). Sensory evaluation of apple juice on the fifth day showed no significant difference between the control sample and probiotics. On the tenth day of sensory evaluation the probiotic juice significantly has higher concentration than the control sample. In general, red apple juice in compared with yellow apple juice has more favorable conditions for growth of Lactobacillus acidophilus. There was no desirable effect on sensory properties of apple juice after adding probiotics.                                                                                                      Manuscript profile
      • Open Access Article

        2 - Antibacterial activity of acetonic, ethanolic and methanolic olive leaf extracts of four Iranian cultivars against Escherichia coli
        Maryam Abbasvali Mahmoud Esmaeili Koutamehr Hamdollah Moshtaghi Mohammad Eskandari
        The aim of this study was to evaluate the antibacterial activity of olive leaf extracts from four important Iranian native cultivars (Dezfol, Roghani, Zard and Shiraz) against E. coli. Acetonic, methanolic and ethanolic olive leaf extracts were prepared using a microwav More
        The aim of this study was to evaluate the antibacterial activity of olive leaf extracts from four important Iranian native cultivars (Dezfol, Roghani, Zard and Shiraz) against E. coli. Acetonic, methanolic and ethanolic olive leaf extracts were prepared using a microwave. The extracts were concentrated and lyophilized. The growth inhibition effect of different concentrations of extracts was determined using the broth micro-dilution assay. The growth curves of E. coli during 24 h incubation at 37°C were drawn. The area under the bacterial growth curve and the percentage inhibition of the growth were calculated. Extracts with the concentrations of 5 and 10 mg/ml had no considerable inhibition effect on the bacterium. Acetonic extract of Dezfol and Zard cultivars with the concentration of 20 mg/ml inhibited the bacterial growth 93% and 94.9%, respectively and showed significantly stronger inhibition effect in comparison with acetonic extract of Shiraz and Roghani cultivars. Methanolic extract of Roghani cultivar with the bacterial growth inhibition of 93.4% showed significantly stronger effect in comparison with its acethonic and ethanolic extracts. Ethanolic extracts of four studied cultivars showed no antibacterial effect. Among the four important Iranian native olive leaf cultivars, Dezfol and Zard were found to possess the highest antimicrobial activity and aceton was the best solvent. On the basis of these results, it can be concluded that extracts of the Iranian native olive leaf cultivars could be used as a natural antimicrobial agent in food preservative, ointment and drugs. Manuscript profile
      • Open Access Article

        3 - Antibacterial effect of methanolic extract of Eryngium Caeruleum and Mentha Spicata on Staphylococcus aureus in a food model at 15˚C
        Fatemeh Amini Hamdollah Moshtaghi Maryam Abbasvali
        In this interventional investigation, the effect of methanol extract of Eryngium Caeruleum and Mentha Spicata (5% and 10% v/w) on Staphylococcus aureus in soy cheese at 106 cfu/ml at 15˚C for a period of 15 days was studied. Methanol extract of Eryngium caeruleum and Me More
        In this interventional investigation, the effect of methanol extract of Eryngium Caeruleum and Mentha Spicata (5% and 10% v/w) on Staphylococcus aureus in soy cheese at 106 cfu/ml at 15˚C for a period of 15 days was studied. Methanol extract of Eryngium caeruleum and Mentha Spicata was prepared by soaking dried plants in 85% methanol. Soy cheese was made through coagulating of soy milk by calcium sulfate. Results were analyzed by one-way variance, ANOVA. In 15˚C, Staphylococcus aureus was faced with reduction in both concentrations (5% and 10% v/w) of methanol extract of E. caeruleum i.e. 1 and 2 log, and in both concentrations (5% and 10% v/w) of methanol extract of M. Spicata i.e. 2 and 4 log, respectively. The antibacterial effect of 10% extract of both of the plants on Staphylococcus aureus was more than 5% extract. Generally, results suggested that Eryngium caeruleum and Mentha Spicata have antibacterial effect, on Staphylococcus aureus. Manuscript profile
      • Open Access Article

        4 - Study of antibacterial effect of Lysozyme on Streptococcus iniae and Lactococcus garviea isolated from rainbow trout (O.mykiss) in Chaharmahal-o-Bakhtiari province
        Masomeh Mohammady Amin Nematollahi Hamdollah Moshtaghi Mojtaba Bonyadian
        The Streptococcus iniae and lactococcus garviea bacteria cause both Streptococcosis and Lactococcosis diseases in the fishes and so in humann. In recent years, these two factors had large break out in fish-breeding farms. This study amid to verify the effect of lysozym More
        The Streptococcus iniae and lactococcus garviea bacteria cause both Streptococcosis and Lactococcosis diseases in the fishes and so in humann. In recent years, these two factors had large break out in fish-breeding farms. This study amid to verify the effect of lysozyme concentration on Streptococcus iniae and Lactococcus bacteria .It also determined minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of these compounds on the growth of mentioned bacteria. Present study investigated antimicrobial effect of different concentrations of Lysozyme (5000, 2500, 1250, 625, 312.5, 156.25, 78.13, 89.06 and 19.53 µg/mL) against Streptoccus iniae and Lactococcus garviea isolated from rainbow trout in Chaharmahal-o-Bakhtiary province. The study used the methods of light (optical) absorption and microdilution in three pH including 5.5, 6, 7, as well. The result of microdilution showed the effect of lysozym in the inhibition of the growth of both bacteria at pH 5.5 and 6 on concentration of 5000 µg/ml, whereas, at pH7 was observed any inhibition. The results showed declining light absorption values resulting from the growth of both bacteria with increasing concentration of lysozyme, however the inhibition effect of 5000 µg/ml of lysosyme at pH6 and 5.5 was significantly (p<0.05) less, in comparison with the smaller concentrations. On the other hand, any inhibitory effect as the optical absorption was observed in the growth of bacteria for the highest concentration of lysozyme in the media of pH 7. Manuscript profile