• Home
  • CHangiz Ahmadizadeh
  • OpenAccess
    • List of Articles CHangiz Ahmadizadeh

      • Open Access Article

        1 - Evaluation of Staphylococcus aureus coagulase polymorphism isolated from food and human samples
        rogayeh bakht avar ahari changiz ahmadizadeh
        Staphylococcus aureus (S. aureus) is one of the significant factors in the development of hospital infections. In the meantime, hospital personnel and foodstuffs are essential factors that can transfer germs to others. Our aim in this study is to determine the frequency More
        Staphylococcus aureus (S. aureus) is one of the significant factors in the development of hospital infections. In the meantime, hospital personnel and foodstuffs are essential factors that can transfer germs to others. Our aim in this study is to determine the frequency of coagulase gene polymorphism in S. aureus isolated from food and human samples. This descriptive cross-sectional study was performed on suspicious samples infected to S. aureus in the microbiology lab of the Faculty of Veterinary Medicine in Islamic Azad University of Tabriz, during the first 6 months of the year 2016. After initial enrichment, the samples were grown in solid media Blood Agar, and Mannitol Salt agar was cultured. To confirm the presence of coagulase gene, Coa G2 and Coa G3 primers were used: Coagulase gene genotyping was performed by the RFLP method. For this purpose, the PCR product was digested in the presence of the enzyme ALUI. The PCR product was digested by agarose gel after digestion. A total of 63 samples, including 11 samples of samples and 52 food samples of 10 hamburgers and 7 samples of chicken and 11 samples of milk 8, cheese samples and 12 samples of ice cream and 1 sample of fish and 3 specimens of the quail were studied. Among them, 35 No genotypes were detected, and 28 samples of S. aureus were found. The results showed that no significant relation was observed between sample types and genotyping of isolated S. aureus strain. that needs further investigation. Manuscript profile
      • Open Access Article

        2 - Comparative study of antibacterial effects of polar, semi-polar, and nonpolar fractions of Rosa canina extract against some pathogenic bacteria
        sanaz tahmasebi changiz ahmadizadeh
        Due to the increasing demand for food worldwide, people inadvertently or deliberately, through the production, supply, import, and export of spoiled and contaminated food, cause instantaneous diseases, short-term, long-term, and even many deaths. Rose canina is one of t More
        Due to the increasing demand for food worldwide, people inadvertently or deliberately, through the production, supply, import, and export of spoiled and contaminated food, cause instantaneous diseases, short-term, long-term, and even many deaths. Rose canina is one of the most valuable medicinal plants that most people use in their lands to treat some diseases. The purpose of this study was to investigate the antimicrobial effects of polar, semi-polar, and non-polar fractions of Rosa canina L. extract on Pseudomonas aeruginosa, Staphylococcus aureus, Listeria monocytogenes, and E. coli. In this study, Rosa canina was used. First, methanolic, chloroform and hexane extracts of R.canina were extracted, and the effects of different concentrations of these extracts were investigated. All experiments were carried out using the well-diffusion method and MIC and MBC determination on standard strains of Intended bacterias. The chloroform extract of the Rosa canina prevented at a concentration of 6.25 μg / ml has an inhibitory effect on Gram-positive bacteria in Listeria monocytogenes. While the impact on gram-negative bacteria of Pseudomonas aeruginosa and Escherichia coli is required for high concentrations of chloroform extract. Methanolic extract of Rosa canina at a concentration of 25 μg / ml has an inhibitory and killing effect on both gram-positive bacteria and gram-negative bacteria, and the chloroform extract of this plant has a most inhibitory effect on Listeria monocytogenes. In general, it can be concluded that the extract of Rosa canina has an antibacterial effect. Manuscript profile