Comparative study of antibacterial effects of polar, semi-polar, and nonpolar fractions of Rosa canina extract against some pathogenic bacteria

Subject Areas : Food Microbial Contamination

sanaz tahmasebi ¹ , changiz ahmadizadeh ²

1 - department of microbiology ahar branch islamic azad university islamic azad university
2 - Department of Microbiology, Faculty of Basic Sciences, Ahar Branch, Islamic Azad University, Ahar, Iran.

Received: 2019-09-21 Accepted : 2019-12-21 Published : 2020-06-21

Keywords: Rosa Canina, plant extract, Non-polar fraction, polar, semi-polar,

Abstract :

Due to the increasing demand for food worldwide, people inadvertently or deliberately, through the production, supply, import, and export of spoiled and contaminated food, cause instantaneous diseases, short-term, long-term, and even many deaths. Rose canina is one of the most valuable medicinal plants that most people use in their lands to treat some diseases. The purpose of this study was to investigate the antimicrobial effects of polar, semi-polar, and non-polar fractions of Rosa canina L. extract on Pseudomonas aeruginosa, Staphylococcus aureus, Listeria monocytogenes, and E. coli. In this study, Rosa canina was used. First, methanolic, chloroform and hexane extracts of R.canina were extracted, and the effects of different concentrations of these extracts were investigated. All experiments were carried out using the well-diffusion method and MIC and MBC determination on standard strains of Intended bacterias. The chloroform extract of the Rosa canina prevented at a concentration of 6.25 μg / ml has an inhibitory effect on Gram-positive bacteria in Listeria monocytogenes. While the impact on gram-negative bacteria of Pseudomonas aeruginosa and Escherichia coli is required for high concentrations of chloroform extract. Methanolic extract of Rosa canina at a concentration of 25 μg / ml has an inhibitory and killing effect on both gram-positive bacteria and gram-negative bacteria, and the chloroform extract of this plant has a most inhibitory effect on Listeria monocytogenes. In general, it can be concluded that the extract of Rosa canina has an antibacterial effect.