Introduction: One of the characteristics of lactobacilli is their ability to produce biosurfactant compounds. In this research, the biosurfactant of Lactobacillus acidophilus and Lactobacillus casei was used to evaluate the antimicrobial and anti-biofilm potential again
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Introduction: One of the characteristics of lactobacilli is their ability to produce biosurfactant compounds. In this research, the biosurfactant of Lactobacillus acidophilus and Lactobacillus casei was used to evaluate the antimicrobial and anti-biofilm potential against four pathogenic bacteria including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Acinetobacter baumannii. Materials and methods: Biosurfactant extraction was done from Lactobacillus acidophilus and Lactobacillus casei, using the method of determining the minimum growth inhibitory concentration (MIC) was used to determine the antimicrobial potential. Also, the micro titer plate method was used to measure the anti-biofilm potential and the type of biofilm of each strain was determined in the presence of Lactobacillus acidophilus and Lactobacillus casei biosurfactant in different concentrations.Results: Determination of the minimum inhibitory concentration (MIC) for pathogen strains, the highest percentage was observed at the concentration of 125 and 250 mg/ml. The anti-biofilm effect of biosurfactant has been used in the approximate concentrations of 62.5 mg/ml, 125 mg/ml and 250 mg/ml. Determination of antibiotic sensitivity showed the results of sensitive, semi-sensitive and resistant states, but the results obtained for Acinetobacter baumannii shows resistance to all antibiotic discs.Conclusion: The biosurfactant isolated from Lactobacillus acidophilus and Lactobacillus casei had antibacterial properties against pathogenic bacteria, also the biosurfactant of Lactobacilli showed that it has anti-biofilm capability.
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