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Open Access Article
1 - Comparison of structure and genetic diversity of Common Pochard (Aythya ferina) using cytochrome B mitochondrial gene in northern and southern regions of the country
Shabnam chavoshi Jalil Imani hamidReza Rezaei Pargol Ghavam MostafavBackground & Objective: The Common Pochard (Aythya ferina) is one of the most abundant and widely distributed species in the world, among ducks. The aim of this study was to investigate the structure and genetic diversity of Aythya ferina in Iran using cytochrome b MoreBackground & Objective: The Common Pochard (Aythya ferina) is one of the most abundant and widely distributed species in the world, among ducks. The aim of this study was to investigate the structure and genetic diversity of Aythya ferina in Iran using cytochrome b gene. Material and Methodology: For this study, samples were taken from 10 pieces of Aythya ferina that were taken from the north and south of the country. Haplotypic and nucleotide analyzes were performed using MEGA, Papart, DNAsp and Network software. Findings: In general, 7 different haplotypes were identified by examining 8 fragments of 929 pairs of cytochrome b gene of Aythya ferina. Haplotype diversity was calculated 0.818, nucleotide diversity was calculated 0.00149. The value of Nm or the amount of gene flow between the sampling logic was 1.383, which indicates a high gene flow and in other words, a very small gene distance between the populations of this species and its European and Asian populations. Rogers-Harping index, Tajima index and Fu Fs were calculated 0.112, -0.028 and -1.288, respectively. The mismatch distribution diagram is also plotted as a single view, all of which show an indeterminate population history similar to the model of sudden expansion. Discussion and Conclusion: According to the results obtained from the haplotype network and the cognitive tree, as well as a slight genetic difference between the studied populations, there is a possibility of migration of this bird from both Europe and East Asia to Iran. Considering the results of the present study, Iranian wetlands should be recognized as important and key areas and the necessary management and protection measures should be taken in these areas. Manuscript profile -
Open Access Article
2 - Phylogeny and genetic diversity of Indian crested porcupine (Hystrix indica) based on mtDNA cytochrome b in Fars province
AliReza Sahraeian Jahromi Jalil Imani Harsini Hamidreza rezaeiBackground and Objective: In recent years, Hystrix indica as the biggest rodent in Iran has been in conflict with humans regarding the destruction of gardens and agricultural products, and the population of this species has been decreased due to illegal hunting and the MoreBackground and Objective: In recent years, Hystrix indica as the biggest rodent in Iran has been in conflict with humans regarding the destruction of gardens and agricultural products, and the population of this species has been decreased due to illegal hunting and the use of pesticides and chemical poisons. Meanwhile there is not effective information which provide the possibility of developing a management and conservation plan for this species, so the aim of this study is to investigate the structure and genetic diversity of Hystrix indica in Fars province habitats. Material and Methodology: 15 quill and tissue samples of this species were collected in the Fars province habitats. After DNA extraction, mitochondrial cytochrome b gene was amplified in 13 samples by polymerase chain reaction and 912 nucleotide pairs of this gene were sequenced and edited for each sample. Findings: Based on the Maximum Likelihood Estimation of Phylogenetic Tree, all the studied samples are related to the same clade and belong to Hystrix indica species. Among the studied samples, only two different haplotypes were identified and the haplotype diversity was equal to 0.282 and the nucleotide diversity was estimated to be 0.00029. Discussion and Conclusion: the results showed that haplotype diversity of Hystrix indica is low in Fars province, so it is suggested to avoid further environmental crises like disruption of its food chain, while conducting demographic studies in order to develop a conservation and management plan for this species. Manuscript profile -
Open Access Article
3 - Valuation of the genetic diversity of the plant plant, Plantago major L., with ISSR molecular markers in Borujerd region
Narjes Azadbar Zahra sadat Mousavi Khansari Mohammad Mehdi Dehshiri Reza YariObjective: Borujerd region in Lorestan province is one of the best and less known natural habitats of plantago major L. The aim of the current study is to identify its natural habitats in Borujerd region and to evaluate genetic diversity in ecotypes with ISSR molecular MoreObjective: Borujerd region in Lorestan province is one of the best and less known natural habitats of plantago major L. The aim of the current study is to identify its natural habitats in Borujerd region and to evaluate genetic diversity in ecotypes with ISSR molecular markers. Materials and methods: In this research, the genetic diversity of 23 samples from 6 populations of Plantago major L. was studied. The extracted DNA of all 23 samples was done by the kit method. 10 primers with international codes were used for PCR reaction. The presence or absence of the band was stored as a one/zero matrix in the SPSS program. Findings: Out of the total 1632 bands produced, 200 bands (alleles) were polymorphism, which was calculated as 24% of polymorphism. The length of the generated fragments varied between 300 and 1650 bp. Primer P8 produced the most common bands with 61 bands and primer P9 produced the least common bands with 7 bands in the populations. The highest number of amplified bands was 206 bands related to P8 primer and the lowest number of produced bands was 125 bands related to P5 primer. Conclusion: The results show that the ISSR genetic markers, especially the P8 primer, can be used in phylogenetic studies of leek plant. Despite its small area, Borujerd region is suitable for the growth of various ecotypes of the Plantago major L, and therefore the preservation and maintenance of this natural habitat and the propagation of different ecotypes should be considered. Manuscript profile -
Open Access Article
4 - Chemical composition and yield of essential oil from two Iranian species of basil (Ocimum ciliatum and Ocimum basilicum)
Abdollah Ghasemi Pirbalouti Fatemeh Malekpoor Azam Salimi -
Open Access Article
5 - Intra-specific differentiation, genetic variability and their prospect for exploitation in medicinally important plant Black henbane (Hyoscyamus niger L.)
Renu Yadav Raj Kishori Lal Ved Ram Singh -
Open Access Article
6 - Expression and comparison of growth gene (IGF-1) in native and non-native rainbow trout (Oncorhynchus mykiss) in three different sizes
Mohammad Reza Ghomi Maryam Haghi Mahmoud Mohseni Masoud GhaneInroduction & Objective: Fish growth is regulated by many environmental and physiological factors and is shaped by the genetic background of each organism. Insulin-like growth factor I (IGF-I) plays an important role in various biological processes of fish. This stu MoreInroduction & Objective: Fish growth is regulated by many environmental and physiological factors and is shaped by the genetic background of each organism. Insulin-like growth factor I (IGF-I) plays an important role in various biological processes of fish. This study aimed to examine the rate of expression of IGF-I mRNA growth gene at different stages of growth in local and imported rainbow trout in Iran. Materials and Methods: Hence, local and imported trout fish in three sizes of small, medium and large, with a total mean weight and size of 453.66±244.34 gr and 32.25±6.78 cm, respectively, were obtained from the fish breeding center in Guilan province (Rasht). First, 20 mg samples of the liver tissue of the fish were obtained under sterile conditions after anesthesia from both local and imported breeds. RNA extraction and cDNA synthesis were performed according to the RT-PCR standard method in the Genetics Research Laboratory of Tonekabon Azad University. Results: The results showed that the expression of IGF-I gene was significantly different between local and imported breeds so that the highest and lowest gene levels were in the small size of imported breed and medium size of local breed, respectively (P<0.05). However, there was no significant relationship between the amount of gene expression and weight and length of fish (P˃0.05), while IGF-I expression showed a decreasing trend with an increase in the fish size.Conclusion: These findings suggest that the IGF-I gene may play a key role in the growth and development of rainbow trout. Manuscript profile -
Open Access Article
7 - Intra-species genetic diversity between different populations of Meloidogyne javanica on tomato in North Khorasan province useing RAPD -PCR
Ghasem Fadavi Khalajlo Esmat Mahdikhani Moghadam Hamid RouhaniIn order to identify of root-knot nematodes in tomato of North Khorasan province, during 2009-2010, 21 populations of M. javanica collected from roots of tomato plants. Morphological studies on perineal patterns of adult females and morphometrical characters of J2s did MoreIn order to identify of root-knot nematodes in tomato of North Khorasan province, during 2009-2010, 21 populations of M. javanica collected from roots of tomato plants. Morphological studies on perineal patterns of adult females and morphometrical characters of J2s did not revealed conspectus polymorphism between different populations of M. javanica. In order to investigating genetic diversity of M. javanica, purification and multiplication of each population were done on susceptible tomato (Red colud cultivar). Eggs and second stage juveniles were isolated from each root and supposed one population. Genomic DNA extracted Morphological studies were assessed by species specific primers. The genetic diversity of populations were studied by Random Amplified Polymorphic DNA (RAPD) with ten 10 mer primers. After amplification, PCR-products were separated by electrophoresis on 1.7℅ gel. DNA bands were scored as 1(presence band) or 0(absence band) in excel and NTSYS software by UPGMA method and dendrograms were constructed based on cluster analysis. The clustering at the 73℅ disagreement level revealed five groups. Based on the global result, RAPD marker could show 73 percent similarity and 27 percent difference between populations. Manuscript profile -
Open Access Article
8 - Study on gentic diversity of the honey bee, Apis mellifera L. using microstatellite ISSR markers in three provinces of Iran
N. Palvaneh E. Soleyman-Nejadian R. Radjabi F. Yaghout nejadMicrosatellite markers ISSR were used for isolation of Apis mellifera L. from populations of Kurdistan, Khuzestan and Esfahan. DNA was extracted from worker bees by optimized salting method. Reproducing was done by 4 pairs of ISSR Primers. The obtained bands were visibl MoreMicrosatellite markers ISSR were used for isolation of Apis mellifera L. from populations of Kurdistan, Khuzestan and Esfahan. DNA was extracted from worker bees by optimized salting method. Reproducing was done by 4 pairs of ISSR Primers. The obtained bands were visible using 2% agaroz gel & satining with ethidium bromaid. The Bands pattern was recorded by presence (1) or absence (0) method. Total of 45 bands were observed which of 84.52 were diffrent.The cluster analysis based on jaccard similarity cofficient indicated the genetic distance. Populations of Kurdistan & Khuzestan were located in group (I) and Esfahan in group (II). Therefore, populations of Kurdistan and Khuzestan provinces showed the highest similarity. Manuscript profile -
Open Access Article
9 - Investigation of Genetic Diversity of Lactobacilli Isolated From Cow Milk Samples by RAPD-PCR
Hakime GhaleKhani Masoumeh Hajirezaei Artadokht TavakoliThe use of lactobacilli in the production of fermented foods has several thousand years old due to their ability to make the desired changes in the taste and texture of the food, as well as their importance in the human health. Different dairy products can be a source o MoreThe use of lactobacilli in the production of fermented foods has several thousand years old due to their ability to make the desired changes in the taste and texture of the food, as well as their importance in the human health. Different dairy products can be a source of new strains of Lactobacillus. Therefore, molecular identification and their genetic variation can be an effective step in identifying native lactobacilli reserves with specific functional characteristics and applying them to industrial dairy products. The purpose of this study is to isolate lactobacilli from flora in cow's milk of Narmeshir city and study their genetic diversity. For this purpose, 21 milk samples were collected from different regions of the city, and Lactobacilli wereisolated by the conventional phenotypic and biochemical methods. In order to screen and investigate the genetic diversity of isolated lactobacilli, five RAPD primers were randomly selected. After doing PCR, the analyses of genetic and phylogenetic diversity were performed using Pyelph software and phylogeny trees were drawn. Cluster analysis of molecular data was able to divide the isolates into seven distinct groups at a genetic distance of 10. K3 and K4 isolates were in group 1 and isolates K21, K13, and K11were in group 4. The remaining isolates were placed in separate groups. Since K3 and K4 isolates were from a region and K21 and K13 isolates were also collected from a region, their placement seems to be logical in a group. Manuscript profile -
Open Access Article
10 - Evaluation of Genetic Diversity in Some of Canola Cultivars (Brassica napus L.) Using Morphological-agronomic and RAPD Molecular Markers
Mansoor Saljooghianpour SeyyedMahdi Javadzadeh Mohsen MohseniIn order to investigate the genetic diversity of canola cultivars, an experiment was conducted in the form of randomized complete blocks with 9 rapeseed cultivars in 3 replications in Iranshahr region. After culture, DNA extraction was done using CTAB or Dellaporta meth MoreIn order to investigate the genetic diversity of canola cultivars, an experiment was conducted in the form of randomized complete blocks with 9 rapeseed cultivars in 3 replications in Iranshahr region. After culture, DNA extraction was done using CTAB or Dellaporta method with some modifications and 6 pairs of RAPD primers were used for amplification. Also, at the end of the growing season, traits such as plant height, plant fresh weight, plant dry weight, yield and seed oil percentage were measured.The results of the analysis of variance showed that the cultivars have a significant difference at the 1% probability level. Also, there was a positive and significant correlation between the studied traits. Cluster analysis of morphological-agronomical data of rapeseed cultivars in four main groups and cluster analysis of molecular data of rapeseed cultivars in three main groups. Cultivars placed in each group have more genomic similarity and cultivars placed in different groups have more genetic differences. In the analysis of the main coordinates of the molecular data, the first two components could not include all the information obtained, so this information indicates the wide distribution of these markers on the rapeseed genome and can cover a wide area of it. The results of this research showed that according to the diversity observed in the cultivars and based on the results of the grouping of genotypes, it is possible to select genotypes from groups that have a greater genetic distance from each other and use them for specific breeding purposes. Manuscript profile -
Open Access Article
11 - Genetic diversity among the different isolates of Nucleopolyhedrovirus in Mazandaran and Golestan provinces using RAPD molecular marker
Marzieh Shazdehahmadi Hoda Assemi Zein-Al-Abedin Shahadatimoghadam Seyed Afshin SajjadiTobacco budworm (Helicoverpa armigera) is one of the key pests of tobacco farms. Nucleopolyhedrovirus is one of the most important biological control agents of budworm and a number of other species. In this study the genetic diversity of different isolates of Nucleopoly MoreTobacco budworm (Helicoverpa armigera) is one of the key pests of tobacco farms. Nucleopolyhedrovirus is one of the most important biological control agents of budworm and a number of other species. In this study the genetic diversity of different isolates of Nucleopolyhedrovirus belonging to 16 natural populations in Mazandaran and Golestan provinces, were investigated. The tobacco budworm samples were collected from tobacco fields and transferred to biotechnology Laboratory of Tirtash Research and Education Center. Purification of NPV virus and DNA extraction was done using CTAB method. Among ten pair of primers which we used for amplification of NPV, two pair of RAPD primers that showed the highest Polymorphism were selected. Sixteen DNA samples isolated from different geographical regions of Golestan and Mazandaran provinces were checked with these selected RAPD primers and they created countable bands. Totally, 87 Countable bands were generated following the PCR products. Among them,75 bands(86%) were polymorphic. Data analysis was done using the PyElph Software. Population grouping using UPGMA method based on genetic distance of 20, revealed five major clusters. Using the underlying RAPD marker, the isolates of Nucleopolyhedrovirus were in a one gene cluster, showed the greatest genetic affinity. Manuscript profile -
Open Access Article
12 - Genetic diversity of Fusarium oxysporum f.sp. cepae the causal agent of onion root and basal rot disease
Bahar Haghani Mahdi Nasr Esfahani Mohammad TorabiRoot and basal rot caused by Fusarium oxysporum f.sp. cepae is one of the most important diseases of onions that causes significant economic losses in onions fields. To recognize the genetic diversity and relationship among isolates of the pathogen, 30 isolates which ha MoreRoot and basal rot caused by Fusarium oxysporum f.sp. cepae is one of the most important diseases of onions that causes significant economic losses in onions fields. To recognize the genetic diversity and relationship among isolates of the pathogen, 30 isolates which had been collected from Isfahan, Kerman, Ardebil and Fars provinces, Iran were studies in 2012. Pathogenicity diversity of the isolates was investigated by inoculation of all isolates on three cultivars of onion named Sefid Kashan, Sefid Ahvaz and Ghermez Azarshahr in greenhouse. Genetic diversity of the isolates was also investigated by RAPD and PCR-RFLP techniques using ITS1 / ITS4 primers. Molecular data were analyzed by NTSYS-pc software and isolates were then classified using Jaccard coefficient and UPGMA simulated methods. In pathogenicity experiment, onion bulbs infected with isolates were planted in pots and maintained in greenhouse with 25 ˚C, 70% RH and 12h light. The experiment was carried out in completely randomized design with five replications and disease severity for each isolate were assessed three weeks after planting. The results showed that disease severity of isolates was significantly different at 1% level of probability in different onion cultivars but no significant relationship was detected in pathogenicity variation and genetic diversity among the isolates. Manuscript profile -
Open Access Article
13 - Investigating Seed Yielding Ability and Its Related Traits of Alfalfa Genotypes (Medicago sativa)
P. Hazeg Jafari F. Nourmand Moayed G. Mohammadi S. Aharizad P. BehrouzIn order to study genetic diversity of traits seed yield, 49 alfalfa genotypes including foreign and Iranian germplasms in 2009, were evaluated in a simple lattice design (7*7) with two replications at the Natural Resources and Agricultural Research Center of East Azerb MoreIn order to study genetic diversity of traits seed yield, 49 alfalfa genotypes including foreign and Iranian germplasms in 2009, were evaluated in a simple lattice design (7*7) with two replications at the Natural Resources and Agricultural Research Center of East Azerbaijan, in Iran. Analysis of variance revealed significant genetic diversity among genotypes with respect to some of the traits. Seed yield correlated significantly with number of days to flowering, seeding period, biomass and harvest index. But, its correlation with number of days to complete flowering and the number of days to seed setting was significant and negative. Plant height, thousand seed weight and biomass were the most important contributors to seed yield. Direct effect of biomass and indirect effect of plant height through biomass on seed yield was high. Therefore, biomass could be used as effective criteria for selection of high yielding genotypes. Manuscript profile -
Open Access Article
14 - Evaluation of Genetic Diversity in 65 Genotypes of Potato by Using Factor and Cluster Analysis
D. HassanPanahTo evaluate genetic diversityamong 65 potato genotypes along with Agria, Draga and Marfona cultivars as controls an experiment based augmented design with three blocks was conducted at Agricultural and Natural Resources Research Station of Ardabil, Iran, during two year MoreTo evaluate genetic diversityamong 65 potato genotypes along with Agria, Draga and Marfona cultivars as controls an experiment based augmented design with three blocks was conducted at Agricultural and Natural Resources Research Station of Ardabil, Iran, during two years(2007-2008).During the growing period and after harvest, the traits such as days number till tuberization, growth period, main stem number per plant, plant height, total and marketable tuber number and weight per plant, marketable tuber yield and dry matter percent were measured. The ANONA results showed that there were significant differences among genotypes for main stem number per plant, plant height, total and marketable tuber number and weight per plant, marketable tuber yield, days number till tuberization and dry matter percent.The main stem number per plant, plant height, total and marketable tuber number and weight per plant and marketable tuber yield traits, had high but days number till tuberization and dry matter percent relativelyhighdiversity. Cluster analysis divided the 65 genotypes into four groups.The third group consisted of five genotypes (Caesar, Luca, Kennebec, Satina cultivars and 397007-9) were found to be higher in total and marketable tuber number per plant, marketable tuber yield than over all mean and the controls. In factor analysis, four independent factors represented 73.49% of the total variations. They were named as 1- yield and its components (marketable tuber yield, total and marketable tuber number and weight per plant), 2- the structural traits (plant height and number of main stems per plant), 3- quality (dry matter percent) and 4- phonology (number of days to tuberization) factors, respectively. Manuscript profile -
Open Access Article
15 - Protein Pattern of Bread Wheat Cultivars Before and After Flowering Using SDS-PAGE Technique
Zahra Baghaeifar Mohsen Farshadfar Samira Mostofi Zohreh Shirkhani Mehdi KakaeiProtein patterns of SDS-PAGE have been widely used to determine genetic variation in cereals. This study was carried out at The Payame-Noor University of Asadabad to study genetic diversity and protein pattern in bread wheat cultivars before and after flowering. 13 brea MoreProtein patterns of SDS-PAGE have been widely used to determine genetic variation in cereals. This study was carried out at The Payame-Noor University of Asadabad to study genetic diversity and protein pattern in bread wheat cultivars before and after flowering. 13 bread wheat cultivars were applied to prepare protein patterns based on SDS-PAGE electrophoresis. The highest genetic distance before bolting was between Zare and Gascogen (8 units). The lowest genetic distance before flowering was between cultivars with a distance equal to zero. This result indicates high genetic similarity between wheat cultivars. The most similarity between the cultivars is the value of one, such as Pishgam and Sayson (one means one hundred percent similarity) or Pishgam and Bezustaya (with one). The least similarity is 0.2 between Zare and Gascogen. Based on cluster analysis these 13 bread wheat cultivars were divided into three groups. The results after bolting were largely similar to the protein pattern before blooming. The most similarity between the cultivars is the value of one. Pishgam and Sayson was one hundred percent similar, and Pishgam and Bezustaya as well. The lowest similarity was observed between Zare and Gascogen cultivars with zero and 0.25 between Zare and other cultivars. Based on cluster analysis, 13 wheat cultivars were grouped into four clusters. Zare, Gascogen and Omid cultivars were placed in separate clusters and the rest of the cultivars were classified in one group. Increasing one group indicates the effect of some proteins that are effective in the developmental stages before and after wheat flowering. Manuscript profile -
Open Access Article
16 - Adaptability Evaluation of 104 Potato Hybrids in Ardabil and Alborz Provinces
Davoud Hassanpanah Ahmad Mousapour Gorji Majid Kahbazi Hossein Karbalaei Khiavi Raouf MohammadiThis study was performed to assess potato hybrids for their promising agronomic, and marketability traits and their adaptability to climatic conditions of potato production areas in country. Some 104 potato hybrids selected during five years (2010-2014) along with Saval MoreThis study was performed to assess potato hybrids for their promising agronomic, and marketability traits and their adaptability to climatic conditions of potato production areas in country. Some 104 potato hybrids selected during five years (2010-2014) along with Savalan, Ceaser, Agria and Khavaran cultivars, as controls, were compared in an augment design (preliminary experiment) without replications both at the Agricultural and Natural Resources Research Station of Ardabil and Seed and Plant Institute Improvement of Karaj. These hybrids (104), tested in this experiment, were from 8 breeding populations. One half of the hybrids (52) in Ardabil and the other half (52) in Karaj (a total of 104 hybrids) each were planted in the 4 blocks and each block consisted of 13 hybrids with four control (Agria, Khavaran, Ceaser and Savalan cultivars). During growing period and after harvest the traits like: plant height, main stem number per plant, main stem diameter, tuber number and weight per plant, marketable tuber yield and tuber dry matter percent were measured. Then 81 hybrids were selected as superior hybrids as to their marketable tuber yield and tuber dry matter content. Hybrids selected consisted of 17 hybrids from ♂ Satina × ♀ Luca population, 36 hybrids from ♂ Ceaser × ♀ Luca population, 14 hybrids from ♂ Savalan × ♀ Luca population, 7 hybrids from ♂ Savalan × ♀ Ceaser population, 4 hybrids from ♂ Ceaser × ♀ Savalan population, 1 hybrids from ♂ Satina × ♀ Savalan population and 2 hybrids from ♂ Satina × ♀ Savalan population. Cluster analysis divided 104 hybrids and cultivars into three groups. The first group with 49 hybrids had higher average tuber number per plant, marketable tuber yield and tuber dry matter percent than the remaining hybrids. In factor analysis, three independent factor total explained 73.90% of the variations. These were named as, 1- tuber yield and its components factor (marketable tuber yield, tuber number and weight per plant), 2- the plant structures factor (plant height, number of main stems per plant and main stem diameter) and 3- quality factor (tuber dry matter percent). Manuscript profile -
Open Access Article
17 - Evaluation of Genetic Diversity of Potato Genotypes for Some Traits Irradiated with Gamma Ray in Caesar Cultivar
داود Hassanpanah M. Rahimi S. VedadiThis research was performed to evaluate genetic diversity for some quantitative traits and tuber flesh color of potato genotypes irradiated with gamma ray of Caesar cultivar, at the Agricultural Nuclear Research Institute of Karaj, Ardabil Behparvar Sabalan Company and MoreThis research was performed to evaluate genetic diversity for some quantitative traits and tuber flesh color of potato genotypes irradiated with gamma ray of Caesar cultivar, at the Agricultural Nuclear Research Institute of Karaj, Ardabil Behparvar Sabalan Company and Ardabil Agricultural and Natural Resources Research Station during 2012 and 2013. The plantlets were irradiated with gamma rays at dose of 25 Gy. The plantlets were sub-cultured three cycles in MS culture and then were transferred to the greenhouse. The 3000 plantlets were planted in plastic pots 10×10 cm with Mikskaar peat-moss and punce (1:1 v/v) in the greenhouse. From 3000 plantlets irradiated belonging to 175 genotypes, 1424 mini-tubers waghed 4821.7 g, were selected with selection intensity of 5.8%. Mean minituber numbers and their weights per plant of genotypes were 8.14 and 27.55 grams and in that of control were 4 and 17.4 grams respectively. The selected genotypes along with control (Caesar and Agria cultivars) were planted in an augmented design in 2013. The ANONA results showed that there were significant differences among genotypes for all traits under study. The tuber yield in parental genotypes was 27.42 ton.ha-1 and in genotypes 3-60 ton.ha-1, number of tubers and their weight per plant in parental genotypes were6 and 555 g. and in genotypes 2-28 and 60-1200 g. respectivly. The tuber yield, tuber number and weight per plant, tuber average weight and main stem number per plant had high diversity. In factor analysis, three independent factors explained 83.07% of the total variation. These factors were: 1- yield and its components (tuber yield, tuber number and weight per plant) as the first factor, 2- the tuber uniformity (average tuber weight) as the second factor, and 3- plant structure (p) and height and number of main stems per plant)as the third factor. The selected genotypes (46 genotypes) possessed higher tuber yields, tuber numbers and weight per plant, tuber uniform, shallow eye depth, skin and flesh color of yellow to dark yellow. Manuscript profile -
Open Access Article
18 - The Effect of Heat Stress at the end of Season on Agronomic Characteristics and Yield of Rapeseed (Brassica napus L.) Genotypes in Ramhormoz
Sahar Kolahkaj Mahroo Mojtabaie ZamaniTemperature during flowering and seed filling is a very important indicator for estimating the yield potential of rapeseed. In order to evaluate the effect of heat stress due to delay in sowing date on agronomic characteristics of different rapeseed genotypes (including MoreTemperature during flowering and seed filling is a very important indicator for estimating the yield potential of rapeseed. In order to evaluate the effect of heat stress due to delay in sowing date on agronomic characteristics of different rapeseed genotypes (including, Hyola420, Hyola50, Hyola60, SAN37, SAN34 and Dalgan) two separate experiments on two Conventional sowing date (11th Nov.) and late sowing date (16th Dec.) each in the form of a randomized complete block design with three replications was carried out in the research farm of Islamic Azad University, Ramhormoz Branch, during the growing season of 2016 and 2017. Each sowing date was considered as an environment and the data were finally analyzed by combined analysis. The results showed that with delay in sowing date and the occurrence of heat stress at flowering and seed filling stages, the number of flowers on the main raceme, ratio of siliques produced per flowers produced, number of siliques per plant, and branches per plant, number of seeds per silique, 1000-seed weight, silique length, plant height and reproductive period decreased and led to significant reduction in seed yield. Reducing silique numbers per plant had the highest contribution to reducing seed yield. In conventional sowing date, seed yield in Hyola 420 and SAN 34 was higher than other genotypes. Under heat stress conditions due to delay in sowing, seed yield in Hyola50 followed by SAN34 was higher than other genotypes and Dalgan had the lowest seed yield. The lowest percentage of reduction seed yield was belonged to Hyola50 (55%) and the amount of stress susceptibility index in this genotype indicated that it was semi-tolerant to late season heat stress. In conventional sowing date, genotypes that quickly reached to the flowering stage but had a longer flowering period, had higher yields. In late sowing date, significant negative correlation was observed between the duration of flowering with number of siliques per plant, number of seeds per silique and seed yield. SAN 34 had high yield in both conventional and late sowing dates and is suggested as a suitable genotype for achieving stable yield in Ramhormoz region. Manuscript profile -
Open Access Article
19 - Molecular diversity of Pasteurella multocida isolated from cattle and buffaloes in East Azerbaijan province based on restriction endonuclease analysis
jalal shayegh alireza monadi jalil Dolgari Sharaf In order to increase information about the molecular diversity of Pasteurella multocida isolated from cattle and buffalo, 2 buffalo and 8 cattle isolates were investigated by Restriction Endonuclease Analysis (REA). REA was performed with Hha-I Endonuclease More In order to increase information about the molecular diversity of Pasteurella multocida isolated from cattle and buffalo, 2 buffalo and 8 cattle isolates were investigated by Restriction Endonuclease Analysis (REA). REA was performed with Hha-I Endonuclease which established 2 distinct profiles: I and II. Cattle and buffalo isolates fell into both REA profiles. Contrary to previous studies, the genetic diversity of the isolates was negligible. Considering the similarity of cattle and buffalo isolates is the present study, further studies witch larger samples should be carried out to investigate the possibility of inter-species transmission. Manuscript profile -
Open Access Article
20 - Evaluation of genetic diversity in some bread wheat genotypes under drought stress conditions using germination stage indices
زهرا مروتی محمد نورانیIn order to investigate phenotypic and genotypic correlations between Characteristics affecting the germination stage and genetic variation and estimating the genetic parameters of these indices in Drought and normal conditions, 19 genotypes of bread wheat were cultivat MoreIn order to investigate phenotypic and genotypic correlations between Characteristics affecting the germination stage and genetic variation and estimating the genetic parameters of these indices in Drought and normal conditions, 19 genotypes of bread wheat were cultivated inCompletely randomized design with three replications in the Laboratories, Department of Agronomy and Plant Breeding of Agricultural Research Campus Natural Resources of Razi University of Kermanshah, Iran In the germination stage was evaluated. The results of variance analysis (ANOVA) showed significant differences for the Germination Percentage (GP), coleoptile Length (KL), Mean Germination Time (MGT), Cofficent of Velocity of Germination (CVG), Average Velocity of Germination (AVG) and Vigor index (SV) under stress and normal condition indicating. The presence of a considerable genotypic variation and possibility of selection of drought tolerant genotypes. The presence of a considerable genotypic variation and possibility of selection of drought tolerant genotypes. According to the biplot obtained in the laboratory conditions (germination test) genotype (16) was located in group A. A high positive genetic and phenotypic relation was observed between GP, AVG, SV and RL, which is completely in line with results of the relation between traits by GTbiplot. High heritability and genetic gain were observed for GP, AVG and SV that reflect the additive gene action. Accordingly, the selection method for studied traits in examined genotypes effective. Cluster analysis using Ward method, based on the traits, classified the genotypes in three different groups. Manuscript profile -
Open Access Article
21 - Investigation of genotypic mango (Mangifera indica L.) in south of Iran based on morphological and biochemical indexes
leila fahmideh Samira Omranipour Baratali FakhariIn order to compare the group of 27 cultivars of mangoes in southern of Iran (Kerman and Hormozgan), physiological traits of leaves and fruits, mango measured on the basis of universal descriptor. The results of analysis of variance showed that the effect of genotype on MoreIn order to compare the group of 27 cultivars of mangoes in southern of Iran (Kerman and Hormozgan), physiological traits of leaves and fruits, mango measured on the basis of universal descriptor. The results of analysis of variance showed that the effect of genotype on the traits of chlorophyll b, total chlorophyll, carotenoids, electrolyte leakage, anthocyanins, leaf, pH fruit, soluble solids, vitamin C (ascorbic acid),TSS fruit juices, pH fruits as well as the kernel traits (kernel length, kernel thickness, kernel width, kernel weight and kernel dry weight) was significant in 1% probability level. The results of the comparison of the averages showed that the highest amount of ascorbic acid, total soluble solids and total leaf chlorophyll belonged to the Sindyderi, twin and Kharvest genotype respectively. In average of kernel length and width, Nabati 1 genotype has the minimum with (3/9 Cm) and Anonym 1 the maximum (11/66 Cm), and the Nabati1 genotype and Langra, respectively, had the minimum and the maximum (2/8 and 4/7 Cm) in Between the genotypes studied. Also, the maximum yields of tree were obtained from Snyderi genotype (260 kg), although there were no significant difference with Shahani 2 genotype (240 kg). Cluster analysis based on the average distance between groups (UPGMA), genotypes in the interval of 7/09 in four groups. Manuscript profile -
Open Access Article
22 - Genetic assessment of some populations of the medicinal plant Caraway (Carum carvi) using RAPD and ISSR markers
leila fahmideh Lila Jani pour Bahman Fazeli-NasabRAPD and ISSR markers were used in the present study to determine genetic relationship and distance between some populations of Carum carvi. Amplification of genomic DNA all population using RAPD analysis yielded 126 fragments, where TIBMBA02 and TIBMBA08 had the minimu MoreRAPD and ISSR markers were used in the present study to determine genetic relationship and distance between some populations of Carum carvi. Amplification of genomic DNA all population using RAPD analysis yielded 126 fragments, where TIBMBA02 and TIBMBA08 had the minimum number of fragments (7) and TIBMBC05 had the maximum number of fragments, (22). ISSR marker yielded 79 fragments, where UBC112 and UBC809had the minimum (1) and maximum (15) fragments, respectively. The Diversity Index value of RAPD primers ranged from 0.8 (TIBMBA02) to 0.94 (TIBMBC05) and also Shanoon Diversity and Nei diversity were 0.57 and 0.38, respectively. In ISSR primers The Diversity Index value ranged from 0.0 (UBC112) to 0.92 (UBC809) and also Shanoon Diversity and Nei diversity were 0.57 and 0.39 respectively. According to ISSR and RAPD markers in combination, maximum genetic similarity (0.48) was observed between Kerman and Jandagh Carum carvi while the minimum similarity (0.115) was observed between Kerman and Neishabour Carum carvi with average 0.315. Also, cluster analysis segregated all populations into 3 groups according to geographical zone. Manuscript profile -
Open Access Article
23 - Phytochemical evaluation of different populations of Tanacetum pinnatum Boiss. in natural habitats using multivariate methods
Fatemehalsadat Serri mohamad bagher Rezaei Abbas ghamarizare Hassanali Naghdi Badi Ali MehrafarinTanacetum pinnatum Boiss. is a perennial plant native to Iran belonging to the Asteraceae family. Which has been used to treat migraine, rheumatism and anti-inflammatory diseases. In this study, phytochemical charchterisic of different populations of T. pinnatum in natu MoreTanacetum pinnatum Boiss. is a perennial plant native to Iran belonging to the Asteraceae family. Which has been used to treat migraine, rheumatism and anti-inflammatory diseases. In this study, phytochemical charchterisic of different populations of T. pinnatum in natural habitats was evaluated by multivariate methods. First, the aerial parts of plant in blooming were collected from Tehran, Alborz, Hamedan and Kermanshah provinces in mid-June to mid-August 2016. The essential oils were extracted by hydro-distillation clevenger device and were analysed by using GC/MS. The results were showed that the effect of population on leaf and flower essential oil was significant (P<0.01) which seems to be due to geographical factors and type of climate. According to the results, 50 compounds were identified in the flower and leaf essential oils of the populations, of which 13 compounds had the highest frequency among the populations and the highest percentage in the leaf and flower essential oils. Based on the principal component analysis and cluster analysis, populations 1, 2, 9, 3 and 5 had the highest composition of flower essential oil, while populations 4, 8 and 10 had the weakest values for most essential oil compounds. Populations 6, 7, 11 and 12 also had moderate amounts of compounds in flower essential oil. However, for leaf essential oil, populations 1, 2, 6, 3, 5 and 10 had the highest essential oil composition, while population 4 had the weakest values for most essential oil compounds and only had a high amount of cubenol. Populations 9, 12, 7, 8 and 11 also had moderate amounts of compounds in leaf essential oil. Population grouping based on their phytochemical assessment did not match their geographical distribution. However, areas with higher E, Z-geranyl acetate،ß-eudesmol and cubenol levels in flowers and leaves had average annual rainfall and higher altitude, average sunshine, and lower annual temperatures (populations 4 and 1). While in areas where populations had higher levels of α-cadinene in flowers, they had the highest average relative humidity, the lowest rainfall and annual temperature. Population 1 had the highest amount of flower ß-eudesmol and was one of the populations that showed a positive correlation between the amount of flower and leaf essential oil and essential oil components. As a result, the best population in the natural habitat was population 1. Manuscript profile -
Open Access Article
24 - بهبود ژنتیکی درصد اسانس و عملکرد گل خشک با استفاده از انتخاب غیرمستقیم در بابونه آلمانی (Matricaria chamomilla L)
احمدرضا گلپرور عبداله قاسمی پیربلوطیمقدمه و هدف: بابونه آلمانی یکی از مهم ترین گیاهان دارویی در سرتاسر دنیاست. بهبود ژنتیکی درصد اسانس و عملکرد گل خشک و افزایش تولید در واحد سطح از اهمیت زیادی در اصلاح این گیاه مهم دارویی برخوردار می باشد. تعیین بهترین شاخص های انتخاب غیر مستقیم به منظور بهبود ژنتیکی درصد Moreمقدمه و هدف: بابونه آلمانی یکی از مهم ترین گیاهان دارویی در سرتاسر دنیاست. بهبود ژنتیکی درصد اسانس و عملکرد گل خشک و افزایش تولید در واحد سطح از اهمیت زیادی در اصلاح این گیاه مهم دارویی برخوردار می باشد. تعیین بهترین شاخص های انتخاب غیر مستقیم به منظور بهبود ژنتیکی درصد اسانس و عملکرد گل خشک جمعیت های بابونه آلمانی با استفاده از برخی از صفات مورفولوژیک، زراعی و فنولوژیک در این تحقیق مورد بررسی قرار گرفت. روش تحقیق: در این تحقیق از طرح آزمایشی بلوک های کامل تصادفی با سه تکرار استفاده شد. صفات مورد اندازه گیری عبارت از تعداد گل در بوته، عملکرد گل تر در بوته، روز تا غنچه دهی، روز تا 50 و 100 درصد گل دهی، تعداد ساقه گل­دهنده، ارتفاع بوته، تعداد پنجه در بوته، عملکرد گل خشک در بوته و درصد اسانس بودند. نتایج و بحث: نتایج تجزیه واریانس نشان دهنده تفاوت بسیار معنی دار بین جمعیت های مورد بررسی از نظر صفات مورد بررسی بود که حاکی از وجود تنوع ژنتیکی قابل ملاحظه برای این صفات در بابونه آلمانی و امکان بهبود ژنتیکی آن ها از طریق گزینش می باشد. بررسی ضرایب همبستگی نشان داد که اکثر صفات دارای همبستگی مثبت و معنی داری با عملکرد گل خشک می باشند. از سوی دیگر، درصد اسانس با تعداد کمتری از صفات دارای همبستگی مثبت و معنی دار بود. توصیه کاربردی/ صنعتی: با انجام تجزیه علیت مشخص شد که برای بهبود ژنتیکی عملکرد گل خشک در بابونه آلمانی می توان از انتخاب غیرمستقیم برای صفات روز تا 50 درصد گل دهی، تعداد گل در بوته، عملکرد گل تر در بوته و روز تا غنچه دهی به عنوان شاخص های انتخاب به ویژه در نسل های مقدماتی استفاده نمود. در مورد درصد اسانس نیز صفات روز تا 50 درصد گل دهی و تعداد گل در بوته به عنوان بهترین شاخص های انتخاب قابل توصیه می باشند. Manuscript profile -
Open Access Article
25 - ترکیبات شیمیائی اسانس سه اکوتیپ نعناع ( Mentha spicata L.) در استان کهگیلویه و بویراحمد
محمدجواد عادلپور احمدرضا گلپرورمقدمه و هدف: خانواده نعناعیان یکی از مهم ترین تیره های گیاهی است که پراکنش جغرافیائی وسیعی داشته و از اهیمت بسیاری برخوردار می باشد که در دو گروه عمده لامیوئیده و نپتوئیده دسته بندی می شود. جنس منتا دارای تنوع ژنتیکی وسیعی است که به دلیل سطوح مختلف پلوئیدی و تلاقی های ب Moreمقدمه و هدف: خانواده نعناعیان یکی از مهم ترین تیره های گیاهی است که پراکنش جغرافیائی وسیعی داشته و از اهیمت بسیاری برخوردار می باشد که در دو گروه عمده لامیوئیده و نپتوئیده دسته بندی می شود. جنس منتا دارای تنوع ژنتیکی وسیعی است که به دلیل سطوح مختلف پلوئیدی و تلاقی های بین گونه ای به وجود آمده است. لذا امکان گزینش افراد برتر که دارای درصد بالای اسانس باشند وجود دارد. روش تحقیق: این مطالعه در سال زراعی 91-92 به منظور شناسائی ترکیبات اندام های هوائی نعناع (Mentha spicata L.) که از سه منطقه جمع آوری شده بودند صورت گرفت. این مناطق شامل یاسوح، سی سخت و بهرام بیگی در استان کهگیلویه و بویر احمد بود. اسانس با استفاده از کلونجر استخراج شد و سپس به وسیله دستگاه کروماتوگرافی گازی متصل به طیف سنج جرمی ترکیبات متشکله آن مورد شناسائی قرارگرفت. نتایج و بحث: نتایج حاکی از شناسائی 10، 14 و 10 ترکیب عمده در اسانس حاصل از سرشاخه های نعناع در این سه منطقه بود. عمده ترین ترکیبات شامل کاروون (57/74 درصد)، 1و8- سینئول (28/10 درصد)، لیمونن (41/8 درصد) در منطقه یاسوج به دست آمد. در منطقه سی سخت ترکیب پیپریتون اکساید (19/53 درصد)، 1و8- سینئول (47/27 درصد)، بتا-کاریوفیلن (55/3 درصد) و در منطقه بهرام بیگی شامل 1و8- سینئول (79/8 درصد)، کاروون (6/79 درصد) و لیمونن (53/3 درصد) بودند. روی هم رفته منطقه بهرام بیگی از بیشترین میزان کاروون برخوردار بود. توصیه کاربردی/ صنعتی: به طور کلی اکوتیپ های مناطق سی سخت و بهرام بیگی دارای پتانسیل ژنتیکی بالاتری از نظر میزان ترکیبات موثره نظیر پیپریتنون اکساید و کاروون برخوردار بوده و قابلیت بهره برداری در برنامه به زراعی و به نژادی را دارا می باشند. Manuscript profile -
Open Access Article
26 - مروری بر کاربرد نشانگرهای ریزماهواره در ارزیابی تنوع ژنتیکی شترسانان
M.M. Musthafaشترها به عنوان کشتی­های صحرا شناخته شده و کاربردهای مختلفی در جهان دارند. برآورد پارامترهای ژنتیکی نخستین گام در مدیریت منابع ژنتیکی برای حفاظت و استفاده مناسب از آنها است. نشانگرهای ریزماهواره به­ طور وسیعی در گاو، گوسفند، بُز و شتر به کار گرفته شده­اند. ول Moreشترها به عنوان کشتی­های صحرا شناخته شده و کاربردهای مختلفی در جهان دارند. برآورد پارامترهای ژنتیکی نخستین گام در مدیریت منابع ژنتیکی برای حفاظت و استفاده مناسب از آنها است. نشانگرهای ریزماهواره به­ طور وسیعی در گاو، گوسفند، بُز و شتر به کار گرفته شده­اند. ولی مطالعات صورت گرفته در زمینه تعیین خصوصیات ژنتیکی بر روی شترها اندک است. افزایش سریعی در حجم داده­های مولکولی حاصل از جمعیت­های شتر بومی مشاهده می­شود. این موضوع حاکی از آن است که سطح آگاهی از این موضوع در جوامع علمی مناسب است. بر مبنای مطالعات صورت گرفته در استرالیا، کنیا، عربستان سعودی، جزایر قناری، هندوستان، مصر و تونس، تنوع ژنتیکی نشانگرهای ریزماهواره در شترها از سطح بالایی برخوردار است. برای یافتن داده­های هتروزیگوسیتی مفید توصیه می­شود که نشانگرهای ریزماهواره YWLL08، YWLL09، YWLL38، YWLL44، YWLL59، VOLP30، VOLP08، VOLP10، VOLP32، VOLP67، LCA66، CVRL01، CVRL05، CVRL06، CVRL07 و CMS50 استفاده گردد. این نشانگرها سطح بالایی از غنای آللی و محتوای اطلاعات چندشکلی را نشان می­دهند. بنابراین آنالیزهای تنوع ژنتیکی بر روی شترها در آینده می­تواند بر مبنای این نشانگرهای بسیار مفید صورت گیرد. Manuscript profile -
Open Access Article
27 - Genetic Variation within and between Three Iranian Goat Populations Using Nine Microsatellite Markers
P. Gholizadeh M.B. Montazer Torbati A. Javanmard S. Alijani -
Open Access Article
28 - آنالیز فرود ژنی بنیانگذاران و تنوع آللی در یک جمعیت تحت انتخاب بلدرچین ژاپنی
م. خالداری ح. قیاسیاین مطالعه برای بررسی آنالیز شجره و دراپ ژنی جهت نمایش تنوع ژنتیکی و توصیه یک استراتژی انتخاب برای بهبود وزن سینه در یک جمعیت بلدرچین ژاپنی انجام شد. تعداد 312 پرنده به دو گروه مساوی تقسیم شدند. یک لاین (S1) برای وزن چهار هفتگی بدن بر اساس ارزشهای اصلاحی و دیگری (S2) ب Moreاین مطالعه برای بررسی آنالیز شجره و دراپ ژنی جهت نمایش تنوع ژنتیکی و توصیه یک استراتژی انتخاب برای بهبود وزن سینه در یک جمعیت بلدرچین ژاپنی انجام شد. تعداد 312 پرنده به دو گروه مساوی تقسیم شدند. یک لاین (S1) برای وزن چهار هفتگی بدن بر اساس ارزشهای اصلاحی و دیگری (S2) برای وزن سینه در چهار هفتگی بر اساس انتخاب بین خانوادگی، انتخاب شدند. توزیع فراوانیهای آللی حاصل از بنیانگذاران با استفاده از شجره هر لاین در نرم افزار شبیهساز دراپ ژنی تخمین زده شد. نتایج نشان داد بهبود ژنتیکی خالص برای وزن بدن و وزن سینه در لاینهای S1 و S2 به ترتیب 3/28 و 7/9 گرم در مقابل 3/23 و 8/6 گرم بود. میانگین تعداد آللهای باقیمانده برای آخرین نسل لاینهای S1 و S2به ترتیب 6/59 و 2/31 بود که به ترتیب 1/19 و 10 درصد کل آللهای اختصاص یافته به جمعیت پایه هر لاین بود. میتوان نتیجهگیری نمود که برای تداوم پاسخ به انتخاب جهت بهبود وزن سینه، انتخاب غیر مستقیم وزن بدن بر اساس ارزشهای اصلاحی قابل توصیه است. نتایج حاصل از آنالیز دراپ ژنی آشکار نمود که در این تحقیق انتخاب بین خانوادگی پیشرفت ژنتیکی بیشتر و تنوع ژنتیکی باقیمانده بیشتری نسبت به انتخاب انفرادی بر اساس ارزشهای اصلاحی ندارد. Manuscript profile -
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29 - بررسی تحقیقی تنوع ژنتیکی سارگپه معمولی ایرانی (Buteo buteo) با استفاده از شناساگرهای ریزماهواره ای
ا. لواف ط. فرهوش م. طرح سازسارگپه معمولی (Buteo buteo) پرنده­ای شکارچی است که در اروپا و آسیا پراکندگی فراوانی دارد. B. buteo ایرانی که نقش مهمی را در چرخه اکولوژیکی نواحی شمالی و شمال شرقی ایران ایفا می­ نماید، در سال­ های اخیر با مشکل شکار بی­ رویه و غیرقانونی مواجه شده­ است Moreسارگپه معمولی (Buteo buteo) پرنده­ای شکارچی است که در اروپا و آسیا پراکندگی فراوانی دارد. B. buteo ایرانی که نقش مهمی را در چرخه اکولوژیکی نواحی شمالی و شمال شرقی ایران ایفا می­ نماید، در سال­ های اخیر با مشکل شکار بی­ رویه و غیرقانونی مواجه شده­ است. به­ منظور بررسی و برآورد تنوع ژنتیکی این پرنده به کمک 10 مارکر میکروستلایت، نمونه ­های خون از 50 بهله B. buteo که در مرکز حفاظت حیات وحش پارک پردیسان تهران، ایران نگهداری می­شدند، جمع ­آوری گردید. جمعیت مورد بررسی جمعیتی تصادفی شامل پرندگانی از مناطق زیست مختلف B. buteo در ایران بود. نتایج این مطالعه نشان ­دهنده تنوع ژنتیکی بالا در جمعیت مورد مطالعه بود و به ترتیب بالاترین و پایین­ ترین مقدار هتروزیگوسیتی در جایگاه­ های Bbu30 و Bbu33 (0.860 و 0.514) به ترتیب مشاهده شد. مقدار هتروزیگوسیتی مورد انتظار (He) در محدوده 0.638 در جایگاه Bbu22 تا 0.832 در جایگاه Bbu30 متغیر بود. کمترین تعداد الل (Na) و تعداد موثر الل (Ne) به ترتیب در جایگاه ­های Bbu22 و Bbu30 مشاهده شد. در جایگاه Bbu17 و Bbu22 به ترتیب بیشترین و کمترین مقادیر آماره­های F محاسبه شد. در جمعیت مورد بررسی انحراف معنی ­داری از تعادل هاردی واینبرگ مشاهده نشد. مطالب بیانگر این مطلب بود که جمعیت B. buteo ایرانی ساکن در پارک پردیسان دارای تنوع ژنتیکی مناسبی بوده و اطلاعات حاصل از مارکرهای میکروستلایت برای ارزیابی مقدار تنوع ژنتیکی و همچنین تکنیک­های مدیریت ژنتیکی این گونه بسیار مفید می­باشند. Manuscript profile -
Open Access Article
30 - بررسی تنوع ژنتیکی و روابط فیلوژنتیکی گوسفندان ایرانی بر اساس توالی ژن سیتوکروم b
س. ساور سفلی ح.ر. سیدآبادی ع. جوانروح علیآباد ر. سید شریفیروابط فیلوژنتیکی و تنوع ژنتیکی در دو نژاد گوسفند ایرانی بر اساس ژن سیتوکروم b مورد بررسی قرار گرفت. DNA ژنومی با استفاده از روش نمکی استخراج و با استفاده از روش PCR، ژن سیتوکروم b تکثیر شد. بخشی از ژن سیتوکروم b به طول 780 جفت باز، تعیین توالی و 13 ناحیه چند شکل و 11 ها Moreروابط فیلوژنتیکی و تنوع ژنتیکی در دو نژاد گوسفند ایرانی بر اساس ژن سیتوکروم b مورد بررسی قرار گرفت. DNA ژنومی با استفاده از روش نمکی استخراج و با استفاده از روش PCR، ژن سیتوکروم b تکثیر شد. بخشی از ژن سیتوکروم b به طول 780 جفت باز، تعیین توالی و 13 ناحیه چند شکل و 11 هاپلوتایپ در نمونه­های مورد مطالعه، مشاهده شد. آنالیز فیلوژنتیکی براساس مقایسه هاپلوتایپ­ها با سایر نژادهای ثبت شده در پایگاه اطلاعاتی، نشان داد که گوسفندان ایرانی در شاخه­ای مجزا قرار دارد. این مطالعه اطلاعات مفیدی در خصوص درک بهتر از روابط بین نژادهای مختلف ارائه می­دهد. همچنین این مطالعه اطلاعات مفیدی جهت درک بهتر ساختار ژنتیکی و تفاوت­های نژادی، برای تببین استراتژی­های اصلاح نژادی و برنامه حفاظت از منابع ژنتیکی دامی در اختیار محققین و اصلاحگرها قرار می­ دهد. Manuscript profile -
Open Access Article
31 - Genetic Diversity and Maternal Origin of Indigenous Sheep of Bangladesh Using Mitochondrial DNA
M.A. Mousumee M.M. Hossain M.R. Hoque M.S.A. Bhuiyan -
Open Access Article
32 - The Complete Mitochondrial Genome from Iraqi Meriz Goats and the Maternal Lineage Using Whole Genome Sequencing Data
S.I. Mustafa J.S. Heslop-Harrison T. Schwarzacher -
Open Access Article
33 - تغییرات ناحیه D-Loop از رشته DNA میتوکندریایی در نژادهای گوسفند بومی ایران
پ. رافیا ا. ترنگDNA میتوکندریایی به دلیل دارا بودن خصوصیات منحصر به فردی همچون توارث مادری، نرخ تکاملی نسبتاً سریع و عدم داشتن نوترکیبی یک ابزار مفید برای مطالعه ژنتیک جمعیت می ­باشد. 82 حیوان غیرخویشاوند از 10 نژاد گوسفند بومی ایران برای تعیین تنوع ژنتیکی مادری با استفاده از یک قط MoreDNA میتوکندریایی به دلیل دارا بودن خصوصیات منحصر به فردی همچون توارث مادری، نرخ تکاملی نسبتاً سریع و عدم داشتن نوترکیبی یک ابزار مفید برای مطالعه ژنتیک جمعیت می ­باشد. 82 حیوان غیرخویشاوند از 10 نژاد گوسفند بومی ایران برای تعیین تنوع ژنتیکی مادری با استفاده از یک قطعه 685 جفت بازی در ناحیه D-Loop از DNA میتوکندریایی، مورد مطالعه قرار گرفتند. از آنالیز این ناحیه درمجموع 72 هاپلوتایپ و 123 موتاسیون تشخیص داده شد. تنوع هاپلوئیدی، تنوع نوکلئوتیدی و میانگین تعداد تفاوت­های نوکلئوتیدی به ترتیب 003/0 ± 996/0، 0001/0 ± 037/0 و 23/25 برآورد شد. آنالیز توالی، سطح بالایی از تنوع ژنتیکی را در میان نژادهای گوسفند بومی نشان داد. تجزیه واریانس مولکولی نشان داد که 43/3 درصد از تنوع به بین جمعیت­ها و 57/96 درصد تنوع به درون جمعیت­ها مربوط می­شود. درخت نزدیکترین همجواری (NJ)، 4 هاپلوتایپ (A، B، C و E) از 5 نوع هاپلوتایپ توصیف شده در گوسفند را نشان داد. درخت فیلوژنتیکی هیچگونه ساختار ژنتیکی متمایزی را در میان جمعیت­های مورد مطالعه نشان نداد، که می­تواند به علت وجود جریان ژنی بالا و آمیختگی بین جمعیت­های گوسفند باشد که احتمالاً به واسطه نقل و انتقال وسیع گوسفند در طول تاریخ و وجود شجرههای مادری مشابه در میان نواحی مختلف باشد. Manuscript profile -
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34 - ارزیابی ملکولی ژن میوستاتین در چهار نژاد بومی بز ایران هیچ رابطه و پیوستگی را با نرخ دوقلوزایی نشان نداد
ع. عبدالمحمدی ک. خانی ص. فروتنی فر ع. زبرجدی ل. سیمایی م. گلیهدف این مطالعه ارزیابی ژن میوستاتین در چهار نژاد بز شامل مهابادی، مرغز، لری و بیتال با روش­های PCR-RFLP و توالی­یابی بود. یک قطعه 573 جفت بازی از ژن میوستاتین با PCR تکثیر شد. با روش توالی­یابی، یک حذف ­شدگی 5 جفت بازی (206TTTA) در ناحیه 5`UTR ژن میوستات Moreهدف این مطالعه ارزیابی ژن میوستاتین در چهار نژاد بز شامل مهابادی، مرغز، لری و بیتال با روش­های PCR-RFLP و توالی­یابی بود. یک قطعه 573 جفت بازی از ژن میوستاتین با PCR تکثیر شد. با روش توالی­یابی، یک حذف ­شدگی 5 جفت بازی (206TTTA) در ناحیه 5`UTR ژن میوستاتین شناسایی گردید. ژنوتیپ­های AA و AB در همه نژادهای فوق، ولی ژنوتیپ­های BB فقط در نژاد مهابادی یافت شدند. ژنوتیپ AA دارای بالاترین فراوانی در همه نژادها بود. از سوی دیگر، ژنوتیپ BB در نژاد مهابادی دارای کمترین فراوانی بود. در چهار نژاد بز مورد بررسی در این مطالعه، فراوانی آلل A بالاتر از آلل B بود. فراوانی آلل A در دو زیرگروه نژاد مهابادی (برای تعداد بزغاله در زایش) تفاوت معنی­داری داشت. این تفاوت در فراوانی آللی در نژادهای لری و مرغز معنی­دار نبود. همچنین آماره­های نسبت بخت (ORs) برای نرخ دوقلوزایی، تفاوت­های معنی­داری را بین ژنوتیپ­ها نشان نداد. ارزیابی ژن میوستاتین حضور دو جعبه TATA (عناصر مهم تنظیمی) در ناحیه پروموتور (موقعیت­های60-56، 32-36)، و یک جعبه E (موقعیت 42-48) را نمایان کرد. به طور کلی، نتایج ما نشان داد که چندشکلی در ناحیه 5`UTR ژن میوستاتین نمی­تواند یک فاکتور کلیدی مهم در بروز دوقلوزایی در این نژادهای بومی بز باشد. به نظر می­رسد مطالعات بیشتری در جمعیت­های دامی بزرگ جهت بررسی تأثیر ژن میوستاتین روی دوقلوزایی، با توجه بیشتر روی جهش­های دیگر ژن میوستاتین یا ژن­های دیگر، مورد نیاز است. Manuscript profile -
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35 - تنوع ژنتیکی بین گاوهای Bali (Bos javanicus) و هیبریدهای آنها با استفاده از نشانگرهای ریزماهواره
ج. جاکاریا ا. آلیاه ف. سپوترا م. بیهقی ر.ر. نورهدف از این مطالعه ارزیابی تنوع ژنتیکی گاوهای Bali و گاوهای هیبرید با استفاده از نشانگرهای ریزماهواره بود. نمونههای خون (192n=) از نژادهای گاو متفاوت جمع­آوری شد، برای نمونه Bali (n=96)، Madura (n=46)، وPeranakan Ongole (PO) Kebumen (n=48). جایگاه ژنی ریزماهواره است Moreهدف از این مطالعه ارزیابی تنوع ژنتیکی گاوهای Bali و گاوهای هیبرید با استفاده از نشانگرهای ریزماهواره بود. نمونههای خون (192n=) از نژادهای گاو متفاوت جمع­آوری شد، برای نمونه Bali (n=96)، Madura (n=46)، وPeranakan Ongole (PO) Kebumen (n=48). جایگاه ژنی ریزماهواره استفاده شده برای دستیابی به تنوع ژنتیکی INRA035، ILSTS006، ETH225 و HEL9 بود، اگرچه پروفایل ژنتیکی با استفاده از برنامههای GenAlEX، Cervus، MEGA6، structure و R توضیح داده شدند. به عنوان یک نتیجه، 46 آلل در چهار لوکوس ریزماهواره مطالعه­شده یافت شد. تنوع ژنتیکی گاوهای Bali از جزیره Bali (H0=0.337) و جزیره Nusa Penida (H0=0.375) پایینتر از هیبریدها رکوردبرداری شدند، برای نمونه گاوهای نژاد Madura (H0=0.474) و PO Kebumen (H0=0.567). اختصاصاً، ما یافتیم 283 جفت باز (لوکوس ILST006)، 194 جفت بازی (لوکوس ETH225)، 147 جفت بازی و 151 جفت بازی (لوکوس HEL9) نشان دهنده آللهای متمایز برای گاوهای Bali. همچنین، دادههای آزمایشگاهی ما نشان داد که نشانگرهای ریزماهواره استفاده شده اجازه میدهد به ما برای تولید یک تفرق آشکار از خوشه گاوها بین گاوهای Bali و هیبریدها، که برای برنامههای اصلاح نژادی آینده گاوها معنیدار بود. Manuscript profile -
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36 - Genetic Diversity Analysis of Four Sheep Breeds of Iran: Towards Genetic Maintenance and Conservation Decision
H. Alnajm S. Alijani A. Javanmard S.A. Rafat K. Hasanpur -
Open Access Article
37 - تمایز ژنتیکی نژاد بومی دراا و ارتباط آن با سایر جمعیتهای بُز با استفاده از نشانگرهای ریزماهواره
ام. ایبنلبشیر ال. کلی آی. بوجنانه آ. چیخی آ. نبیچ ام. پیروجمعیت­های مختلف بُزهای مراکشی تنها بر مبنای فنوتیپشان شناخته می­شوند. هدف این مطالعه، ارزیابی تمایز ژنتیکی بُز نژاد دراا و آنالیز ساختار ژنتیکی آن و رابطه­اش با سایر جمعیت­های بومی با استفاده از 12 نشانگر ریزماهواره است. این پژوهش در جنوب و جنوب شرقی مرا Moreجمعیت­های مختلف بُزهای مراکشی تنها بر مبنای فنوتیپشان شناخته می­شوند. هدف این مطالعه، ارزیابی تمایز ژنتیکی بُز نژاد دراا و آنالیز ساختار ژنتیکی آن و رابطه­اش با سایر جمعیت­های بومی با استفاده از 12 نشانگر ریزماهواره است. این پژوهش در جنوب و جنوب شرقی مراکش روی 192 حیوان از پنج جمعیت شامل دراا، اطلس، بارکا، قازالیا و از مجموعه­ای از بزهایی که فنوتیپ­های بسیار متغیری داشته و با یکدیگر در جمعیت بز­های نامشخص دسته­بندی می­شوند، صورت گرفته است. ساختار جمعیت با استفاده از شاخص­های تنوع استاندارد، آنالیز واریانس مولکولی و تکنیک­های خوشه­بندی بیزی مرود مطالعه قرار گرفت. بیش از 4/88 درصد از واریانس کُل بین افراد توزیع شده و تنها 85/1 درصد ناشی از تفاوت­های بین جمعیت­ها بوده است. نژاد دراا پایین­ترین هتروزیگوسیتی مشاهده شده (579/0)، بالاترین ضریب همخونی (161/0) و انحراف بیشتری از تعادل هاردی-واینبرگ نشان داد. علاوه بر این، این نژاد بیشترین فاصله ژنتیکی را از سایر جمعیت­ها داشته است. خوشه­بندی بیزی نشان داد که سطح بالایی از اختلاط بین جمعیت­ها وجود داشته و یک خوشه منفرد قابل شناسایی نیز در داخل نژاد دراا وجود دارد. نتایج نشان می­دهند که جمعیت­های بُز مراکشی تنوع ژنتیکی قابل ملاحظه­ای داشته ولی در عین حال ساختار ژنتیکی ضعیفی دارند. در این بین، نژاد دراا یک استثنا بوده که درجه بالاتری از تمایز و ساختاربندی جمعیتی را نشان می­دهد. Manuscript profile -
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38 - بررسی ساختار جامعه و همخونی گوسفند سنگسری
م. میرزایی ایلالی س. حسنی م. آهنی آذری ر. عبدالهپور س. نقویانهدف این مطالعه توصیف همخونی و ساختار جامعه در ایستگاه اصلاح نژاد گوسفند سنگسری بود. به این منظور، داده­های 7028 رأس گوسفند سنگسری که در طول سالهای 1365 تا 1392 در ایستگاه اصلاح نژاد گوسفند سنگسری واقع در نزدیکی شهر دامغان، استان سمنان جمعآوری شده بود، مورد استفاده Moreهدف این مطالعه توصیف همخونی و ساختار جامعه در ایستگاه اصلاح نژاد گوسفند سنگسری بود. به این منظور، داده­های 7028 رأس گوسفند سنگسری که در طول سالهای 1365 تا 1392 در ایستگاه اصلاح نژاد گوسفند سنگسری واقع در نزدیکی شهر دامغان، استان سمنان جمعآوری شده بود، مورد استفاده قرار گرفت. برههای متولد شده در سال­های 1388 تا 1392 به عنوان جامعه مرجع درنظر گرفته شدند. آنالیز ساختار ژنتیکی این جامعه با نرم افزار ENDOG نسخه 8/4 انجام شد. میانگین همخونی کل جامعه و جامعه مرجع به ترتیب 28/0 و 70/0 درصد بود. متوسط رابطه خویشاوندی، اندازه جامعه مؤثر تحقق یافته بر اساس افزایش انفرادی در همخونی، تعداد مؤثر بنیان گذاران (fe) و معادل ژنومی حیوانات بنیانگذار (fg) به ترتیب 59/0 درصد، 75/148، 140 و 97/49 تخمین زده شد. میانگین فاصله نسلی در جامعه حاضر 24/4 سال و متوسط تعداد نسل های معادل 64/1 تخمین زده شد. متوسط همتباری، تعداد مؤثر اجداد (fa)، متوسط نسل معادل کامل به ترتیب 0005/1 درصد، 76 و 02/3 محاسبه شد. نسبت fe به fa که بیان کننده اثر باتل نکهای جامعه است، 84/1 بود. پنجاه درصد کل تنوع ژنتیکی مربوط به 32 جد با بالاترین نفوذ بود. با نظارت برپارامترهای مربوط به تنوع ژنتیکی و مدیریت جفتگیری میتوان از کاهش تنوع ژنتیکی و اثرات مضر احتمالی ناشی از افزایش همخونی جلوگیری نمود. Manuscript profile -
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39 - تنوع ژنتیکی ژن سیتوکروم b در سه نژاد بز بومی در جاوه مرکزی اندونزی
س. سوتوپو ا. پوربوواتی ا. کورنیانتواین پژوهش برای توضیح تنوع ژنتیکی و ارتباط فیلوژنیکی سه نژاد بز بومی در جاوه مرکزی اندونزی بر اساس توالی ژن سیتوکروم b (cyt b) انجام شد. نمونههای خون به ترتیب از 19، 15 و 12 رأس از نژادهای Kacang، Kejobong وEttawa Grade، به عنوان مواد پژوهش استفاده شدند. برهمگذاری چندگ Moreاین پژوهش برای توضیح تنوع ژنتیکی و ارتباط فیلوژنیکی سه نژاد بز بومی در جاوه مرکزی اندونزی بر اساس توالی ژن سیتوکروم b (cyt b) انجام شد. نمونههای خون به ترتیب از 19، 15 و 12 رأس از نژادهای Kacang، Kejobong وEttawa Grade، به عنوان مواد پژوهش استفاده شدند. برهمگذاری چندگانه ژن cyt b توسط مقایسه نژادهای بز تحت مطالعه و یک توالی ارائه شده در بانک ژن (کاپرا هیرکوس، شماره دسترسی AB004072) انجام شد. از 16 جایگاه متفاوت جفت بازی یافت شده، 12 جایگاه وجود دارد که میتواند به عنوان نشانگر برای تشخیص سه نژاد بز بومی در اندونزی با کاپرا هیرکوس استفاده شوند. فاصله ژنتیکی درمیان بزهای بومی در مقایسه با فاصله ژنتیکی بین بزهای بومی با توالی ژن بانک کاپرا هیرکوس کاملاً نزدیک بود. درخت فیلوژنی دو خوشه اصلی را نشان داد، که خوشه A برای کاپرا هیرکوس و خوشه B برای سه نژاد بز بومی اندونزی بود. Manuscript profile -
Open Access Article
40 - تعیین خصوصیات مولکولی نژادهای بز بومی عراق با استفاده از نشانگرهای RAPD
Y. Muhammad Salih Al-Barzinj ر. اسمات اوراماری ر. عبد الغنی ال-سنجوریکشمیر تنها توسط نژاد بز مرخز (مرز یا کردی) در ناحیه کردستان عراق تولید می­شود. هدف از این مطالعه بررسی تنوع ژنتیکی تیپ­های رنگی مرخز، بز سیاه و نژادهای شامی با استفاده از نشانگرهای RAPD بود. 14 آغازگر استفاده شده و 9 نشانگر از بین آنها بر مبنای تعداد نوارها (NB) Moreکشمیر تنها توسط نژاد بز مرخز (مرز یا کردی) در ناحیه کردستان عراق تولید می­شود. هدف از این مطالعه بررسی تنوع ژنتیکی تیپ­های رنگی مرخز، بز سیاه و نژادهای شامی با استفاده از نشانگرهای RAPD بود. 14 آغازگر استفاده شده و 9 نشانگر از بین آنها بر مبنای تعداد نوارها (NB) و خصوصیات چندشکلی انتخاب گردیدند. این نشانگرها 154 نوار در محدوده 100 تا 2800 جفت باز تولید کردند. در بین کل نوارها، تنها 50 نوار چند شکل تشخیص داده شدند. 13 نوار منحصر به فرد در بز مرخز شناسایی گردیده و بیشترین تعداد نوارهای منحصر به فرد نیز در آغازگر ژنگاه 7-MO2 به دست آمد. تنوع ژنی کُل نِی (تنوع ژنی تقسیم بر هتروزیگوسیتی) به طور متوسط 40/0 بود. ولی شاخص تنوع شانون برابر با 58/0 (بین 45/0 تا 69/0) بود. در درختان تبارنما سه خوشه مشاهده گردید. خوشه اول شامل نژاد بز سیاه و خوشه دوم شامل نژاد بز شامی با بز سیاه و بز مرخز قهوه­ای روشن بود. خوشه سوم نیز شامل بزهای مرخز سفید و قهوه­ای تیره گردید. تیپ­های رنگی مرخز در یک خوشه که حاوی تیپ­های سفید و قهوه­ای تیره بودند گروه­بندی گردیده و تیپ­های سیاه با قهوه­ای روشن در خوشه دیگری قرار گرفتند. این نتایج نشان می­دهند که نژاد بز مرخز قرابت بیشتری با بز شامی در مقایسه با نژاد بز سیاه دارد. Manuscript profile -
Open Access Article
41 - تنوع میتوکندری و ساختار فیلوژنتیکی جمعیت بز مرخز
ح.ر. سیدآبادی ک. پهلوان افشاری م. عبدالمالکیتنوع میتوکندری و ساختار فیلوژنتیکی جمعیت بز مرخز بر اساس توالی DNA میتوکندریایی مورد بررسی قرار گرفت. آنالیز فیلوژنتیکی بر اساس توالی ناحیه HVR1 ( 968 جفت باز) و بر روی 40 رأس بز مرخز انجام گرفت. نتایج نشان داد که جمعیت بز مرخز دارای تنوع ژنتیکی بسیار بالا ( میانگین تنو Moreتنوع میتوکندری و ساختار فیلوژنتیکی جمعیت بز مرخز بر اساس توالی DNA میتوکندریایی مورد بررسی قرار گرفت. آنالیز فیلوژنتیکی بر اساس توالی ناحیه HVR1 ( 968 جفت باز) و بر روی 40 رأس بز مرخز انجام گرفت. نتایج نشان داد که جمعیت بز مرخز دارای تنوع ژنتیکی بسیار بالا ( میانگین تنوع هاپلوتیپی 999/0 و تنوع نوکلئوتیدی 022/0) می­باشد. در بین 40 نمونه، 6 هاپلوتیپ مشاهده شد و بزرگترین هاپلوتیپ شامل 15 فرد بود. تمام هاپلوتیپ­ها در هاپلوگروپ C و با فاصله ژنتیکی زیاد از سایر جمعیت­های بز آسیایی قرار گرفتند که این بیانگر تنوع ژنتیکی زیاد بز مرخز می­باشد. تنوع ژنتیکی بالای مشاهده شده، در اصلاح نژاد و تعیین استراتژی­های حفاظت بز مرخز می­تواند مؤثر باشد. Manuscript profile -
Open Access Article
42 - Analysis of the Efficiency for the Pig-Tailing Technique to Dissect the Molecular Parameters Based on Microsatellite Markers: Camels (Camelus dromedaries) as Model
M. Bitaraf Sani N. Asadzadeh R. Nahavandi Z. Roudbari S.A. Rafat A. Mirjalili A. Javanmard -
Open Access Article
43 - تعیین خصوصیات ژنتیکی جمعیت بز هامرا در دو منطقه مختلف موروکو با استفاده از نشانگرهای ریزماهواره
ب. هیلال آی. بوجنانه اس. ال اوتمانی م. چنتوف م. پیرودر این مطالعه، تنوع ژنتیکی دو جمعیت مختلف نژاد بز هامرا در موروکو روی 60 نمونه مختلف (30 نمونه از منطقه بِنی­آروس و 30 نمونه از منطقه رومانی) با استفاده از 15 نشانگر ریزماهواره مورد مطالعه قرار گرفته است. مجموعاً 145 آلل شناسایی گردید و تعداد متوسط آلل به ازای هر جا Moreدر این مطالعه، تنوع ژنتیکی دو جمعیت مختلف نژاد بز هامرا در موروکو روی 60 نمونه مختلف (30 نمونه از منطقه بِنی­آروس و 30 نمونه از منطقه رومانی) با استفاده از 15 نشانگر ریزماهواره مورد مطالعه قرار گرفته است. مجموعاً 145 آلل شناسایی گردید و تعداد متوسط آلل به ازای هر جایگاه، 67/8 و 07/8 آلل به ترتیب در بزهای منطقه بِنی­آروس و منطقه رومانی بوده است. شاخص اطلاعات شانون بین 58/1 در بزهای رومانی تا 66/1 در بزهای بِنی­آروس متغیر بود. میانگین هتروزیگوسیتی مورد انتظار و مشاهده شده کُل جایگاه­ها از 62/0 تا 72/0 در بزهای رومانی و 64/0 تا 75/0 در بزهای بِنی­آروس متغیر بود. شش نشانگر در بزهای بِنی­آروس و پنج نشانگر در بزهای رومانی انحراف معنی­دار از تعادل هاردی - واینبرگ نشان دادند. مقادیر FIS برای بزهای بِنی­آروس و رومانی به ترتیب 110/0 و 108/0 بود. مقادیر FST حاکی از وجود مقادیر اندک تمایز ژنتیکی بین دو گروه بز مزبور بوده است. فاصله ژنتیکی نِی بین دو گروه 046/0 بوده و نشان دهنده پایین بودن تمایز ژنتیکی است. آنالیز واریانس مولکولی (AMOVA) نیز موید این موضوع بوده و نشان می­دهد که 15/99 درصد از تنوع در داخل گروه­های ژنتیکی توزیع گردیده است. حضور دو خوشه (2=K) برای نشانگرهای ریزماهواره نشان دهنده سطح بالای اختلاط این دو جمعیت است. از نتایج حاصل اینطور نتیجه­گیری می­شود که هر دو گروه (بِنی­آروس و رومانی) از شباهت بالایی برخوردار بوده و می­توان آنها را متعلق به یک جمعیت تلقی نمود. Manuscript profile -
Open Access Article
44 - Evaluation of Turkish durum wheat (Triticum turgidum var. durum) genotypes based on quantitative traits and shoot zinc accumulation under zinc-deficient calcareous soil
Ezatollah Esfandiari Majid Abdoli Behzad Sadeghzadeh Seyed-Bahman Mousavi -
Open Access Article
45 - Genetic diversity analysis of White, Red and Chiti Bean under non stress condition.
Shahab Khaghani Shohreh Khaghani Mahdi Changizi Masoud Gomariyan -
Open Access Article
46 - Investigation of genetic diversity among some plant of north Iran tea germplasm (Camellia sinensis) using morphological traits.
Arezo MehrAsa hosein Moradi davod bakhshi shahin jahangirzadeh khiaviTea (Camellia sinensis(L.) O. Kuntze) is one of most important crop in north of Iran, Today’s, many of these plants are at risk of disappearing therefore having information about genetic of them can help in designing of breeding programs. In this study, 30 tea bus MoreTea (Camellia sinensis(L.) O. Kuntze) is one of most important crop in north of Iran, Today’s, many of these plants are at risk of disappearing therefore having information about genetic of them can help in designing of breeding programs. In this study, 30 tea bushes by using 21 quantitative and qualitative traits related to the leaves and stems of the tea plant was analyzed for genetic diversity using on Gower distances coefficient and UPGMA algorithm. The results of data analysis showed that all the selected tea samples were morphologically similar to each other, although some of them showed small and slight differences in some characteristics, but overall they did not have significant differences. Based on the obtained data, the difference between the studied samples ranged from 0.048 to 0.499 with an average of 0.265. In order to determine the relationship of the examined genotypes and grouping them based on the morphological traits in cluster analysis at the distance coefficient of 0.29, the examined samples were grouped into four main groups. According to the way of grouping, it was observed that the distribution of the studied samples does not follow a specific pattern and does not match with the geographical distribution. According to the results of the present research, it is confirmed that morphological analyzes in tea can be used successfully to determine genetic diversity and relationship. The results of this study open a window to deal with the old problem of classification and identification of tea in Iran. Manuscript profile -
Open Access Article
47 - Assessment of genetic diversity and phylogenetic relationship of some cultivated (Apium graveolens) and wild celery(Kelussia odoratissima) genotypes using SDS-PAGE
fatemeh Mahmoudi kurdi Ardeshir Joodmand sara ghaffarianThe study of proteins is an appreciate model for assessment genetic diversity and similarity between different genomes. Considering to the medicinal and nutritional importance of Apium graveolens and Kelussia odoratissima in this study genetic relationships and diversit MoreThe study of proteins is an appreciate model for assessment genetic diversity and similarity between different genomes. Considering to the medicinal and nutritional importance of Apium graveolens and Kelussia odoratissima in this study genetic relationships and diversity of 6 Kelussia genotypes based on seed proteins and 15 celery based on proteins of seeds and leaves 30, 45 and 60 days after germination were studied by SDS-PAGE. Presence and absence of the protein bands were scored as one and zero, respectively. The total number of bands, number of polymorphic bands, the percentage of polymorphic bands, number of private bands, number of alleles, number of effective alleles, genetic diversity, mean of heterozygosity, polymorphic information content and Nei gene diversity index were estimated. Also analysis of molecular variance, analysis of molecular variance to two first coordinates and grouping of populations based on minimum evolution algorithm and P-distance were done. Results showed high genetic diversity in both populations but higher heterozygosity and within population diversity in celery seed compare to Kelussia. Grouping of populations separated celery and Kelussia populations. Agreement of populations grouping with geographic dispersion was more strong in seeds than leaves Manuscript profile -
Open Access Article
48 - Genomic fingerprinting of some isolated Azospirillum spp. of wheat and maize fields by using rep-PCR.
Mahmood Shahabi Naser Panjekeh Hadi Asadi Rahmani Mohammad SalariBackground & Objectives: Azospirillum comprises rhizobacteria that live freely in the soil where they change gaseous nitrogen to nitrite and nitrate and as a result promote growth and yield of some economically important plants. This study aimed to isolate and ident MoreBackground & Objectives: Azospirillum comprises rhizobacteria that live freely in the soil where they change gaseous nitrogen to nitrite and nitrate and as a result promote growth and yield of some economically important plants. This study aimed to isolate and identify different native species of Azospirillum and to investigate their genetic diversity as plant growth-promoting factors. Materials & Methods: In this research, some soil and root samples from wheat and maize fields of Isfahan province were collected and cultured in the tubes containing nitrogen-free bromothymol blue (NFB) semisolid media. The Azospirillum isolates were chosen based on morphological properties on NFB and RC (Rojo Congo) media and then were purified. The detection and identification of Azospirillum isolates was done by molecular tests using PCR (Polymerase Chain Reaction), 16S rRNA gene sequencing, and genomic fingerprinting with rep-PCR (repetitive extragenic palindromic elements-polymerase chain reaction). Results: The replication of 646 and 263 bp fragments by specific primers, confirmed the presence of Azospirilum bacteria. Azospirillum species were also identified as A. brasilense, A. lipoferum, and A. zeae using replication and sequencing of the 16S rRNA gene. The genomic profiling of different Azospirillum isolates produced unique band patterns through application of repetitive element BOX (BOX-AIR), ERIC (Enterobacterial Repetitive Intergenic Consensus), and REP and showed high genetic diversity between the isolates. Conclusion: According to this study, the Azospirillum isolates of wheat and maize fields of Isfahan province have different species and genetic diversity and band patterns amplified using rep-PCR can be useful for diagnosis and classification of Azospirillum species. Manuscript profile -
Open Access Article
49 - Genetic diversity of colistin-resistant Acinetobacter baumannii isolates in patients of Shahid Rajaee Trauma Hospital in Shiraz
Shahriar Sepahvand Mahboobeh Madani Mohammad Ali Davarpanah Fereshte GhandehariBackground & Objectives: Acinetobacter baumannii is the clinically significant bacteria easily capable acquiring multi-resistance to antibiotics. This study aims to evaluate the pattern of resistance, colistin resistan MoreBackground & Objectives: Acinetobacter baumannii is the clinically significant bacteria easily capable acquiring multi-resistance to antibiotics. This study aims to evaluate the pattern of resistance, colistin resistance gene, and the study of genetic diversity of colistin-resistant strains isolated from Shahid Rajaee Hospital in Shiraz.Materials & Methods: This descriptive cross-sectional study was conducted on A. baumannii strains isolated from patients admitted to the intensive care unit ICU of Shahid Rajaei Hospital in Shiraz. A. baumannii strains were identified and confirmed by Microgen kit and blaOXA-51 gene. Antibiotic susceptibility testing was then performed by disk diffusion method according to CLSI 2020 instructions. PCR method was used to study pmrA and pmrB genes and also genetic diversity of A. baumannii strains was analyzed by SPSS software and NTSYS version2.10e.Results: Fifty strains of A. baumannii were isolated and identified. These strains had multi-resistance and showed high resistance to most antibiotics. The lowest rate of resistance was observed to colistin and tigecycline antibiotics. In the study of colistin-resistance genes, colistin-susceptible and colistin-resistant strains of A. baumannii carried pmrA and pmrB genes, respectively. Colistin-resistant strains were placed next to colistin-sensitive strains in a node in the dendrogram.Conclusion: The results of the present study revealed the frequency of multi-resistant A. baumannii strains in the hospital. These strains belonged to a common clone and seem to be circulating in the hospital. Infection control programs are required to be implemented in hospital wards. Manuscript profile -
Open Access Article
50 - Molecular typing of β -lactamase -producing Escherichia coli: Multiple Locus variable number tandem repeat analysis Versus Pulsed-field gel electrophoresis
Alireza Dolatyar Dehkharghani Mohammad Rahbar Setareh Haghighat Marjan Rahnamaye FarzamiBackground & Objectives: β -lactamase -producing E. coli is the most important agent causing urinary tract infections both in community and hospitals. Strain typing including MLVA and PFGE are the most common epidemiologically tools not MoreBackground & Objectives: β -lactamase -producing E. coli is the most important agent causing urinary tract infections both in community and hospitals. Strain typing including MLVA and PFGE are the most common epidemiologically tools not only for detecting the cross transmission of nosocomial pathogens but also for determining the source of infection.Materials and methods: The present study was carried of for comparison of discriminatory power of MLVA and PFGE methods. A Total of 230 isolates of E. coli from patients with urinary tract infections were examined for identifying and antimicrobial susceptibility testing MLVA and PFGE methods were used for molecular typing of all isolates.Results: Out of 230 isolates, 130 ( 56.5%) β -lactamase -producing E. coli isolates were found in this study. The diversity indices of the VNTR loci showed an average diversity of 0.48 and 0.54 for 7-loci and 10-loci respectively. The discriminatory power of PFGE showed a value of 0.87. Conclusion: Our study showed that PFGE is more discriminatory than MVLA. MLVA is a PCR- based method and generates unmistakable data, in contrast to PFGE. Optimization of polymorphic VNTR is essential to improve the discriminatory power of MLVA basis on geographical region. Manuscript profile -
Open Access Article
51 - Investigation of Genetic Variations among Crested Wheatgrass Species Base of Agronomical Traits and Total Leaf Protein
Parvin Salehi Shanjani Ali Ashraf Jafari Razieh Jahanbaz -
Open Access Article
52 - Genetic diversity investigation of different grape varieties (Vitis vinifera) using ISSR molecular marker
A. Khalkhali Hossein abbaspourGrapes is one of the most important horticultural products in the world and Iran and has a wide gnetic diversity, so the purpose of this research was to investigate of the genetic diversity and to asses of introduced grapes cultivars (Vitis vinifera) at the molecular le MoreGrapes is one of the most important horticultural products in the world and Iran and has a wide gnetic diversity, so the purpose of this research was to investigate of the genetic diversity and to asses of introduced grapes cultivars (Vitis vinifera) at the molecular level using ISSR marker. For DNA extraction, it was used modified method of Doyle and Doyle and at the next step, 12 genotypes were examined by 12 primers. Cluster analysis was performed by PopGen32 and SPSS9 softwares. The resulted clustering were divided cultivars into 5 groups by both softwares. In analyzing data, the percentage of polymorphism and the number of polymorphic loci were obtained 96.49 percent and 55 respectively. Cophenetic coefficient is 0.8 for Jaccard coefficient and straw, is indicating a fine fit between the dendrogram and the main similarity matrix. The bands that were Obviously visible were reviewed. Results showed that 353 bands were generated by the markers totally. The size of bands was various between 200 and 3000 bp. The highest similarity was 0.619 for number one (sabz Shah Pasand) and number 7 (currants Khalili Ghouchan) varieties and the least one was 0.205 for number10 (Pir Golli 2) and number 2 (Kashmar Mall) varieties. Because the most amount of bond were observed by primer a (56 bands), this primers was able to determine the genetic gap of Primerrelated varieties better than other primers. Manuscript profile -
Open Access Article
53 - Evaluation of genotypic variation for seed iron content and concentration in barley genotypes under dryland conditions
mehdi feizi mahmood solouki behzad sadeghzadeh baratali fakheri seyed abolghasem mohammadiDrought stress and iron (Fe) deficiency are abiotic stress factors limiting crop production and its quality, especially in rainfed areas. With the aim of evaluation of genotypic variation for Fe efficiecny, 121 barley genotypes during the growing season of 2015-2016, a MoreDrought stress and iron (Fe) deficiency are abiotic stress factors limiting crop production and its quality, especially in rainfed areas. With the aim of evaluation of genotypic variation for Fe efficiecny, 121 barley genotypes during the growing season of 2015-2016, a research farm was carried out at the agricultural research institute of the dryland (Maragheh) were studied under rainfed field conditions. . The experiment was conducted in square lattice. Based on ANOVA, there was significant differences among genotypes for seed Fe concentartion and content traits. The high range of variation for these traits showed that there is a great genotypic variation among barley genotypes. The existance of high general heritability (h2) for seed Fe concentration and content could be helpful in breeding for these traits under different environments. Seed Fe concentration had significant and positive correlation (r=0.63**) with seed Fe content. In short, the result of this study revealed the great genotypic variation among barley genotypes for Fe absorption and accumulation in seed. Moreover, the efficient genotypes could alleviate drought stress that will result in higher grain yield with Fe-dense grains under cold dryland conditions. Manuscript profile -
Open Access Article
54 - Evaluation of morphophysiological traits and drought tolerance indices in some advanced durum wheat (Triticum durum L.) lines under supplementary irrigation and irrigation conditions
Fereshteh Jokar Asad Masoumiasl Rahmatollah KarimizadehThe development of food conversion industries has revealed the necessity of increasing the production of durum wheat in the country and, on the other hand, environmental stresses are responsible for decreasing the yield of crops. In order to compare 12 advanced durum wh MoreThe development of food conversion industries has revealed the necessity of increasing the production of durum wheat in the country and, on the other hand, environmental stresses are responsible for decreasing the yield of crops. In order to compare 12 advanced durum wheat lines in drought tolerance, a randomized complete blocks design with four replications in two conditions (without irrigation and supplemental irrigation) in the crop year 1393-94 at Gachsaran dryland research station were conducted and morpho-physiological traits such as plant height, spike length, peduncle length, grain filling period, number of plants per square meter, 1000 seed weight and grain yield were evaluated. Results showed that all assessed traits in genotypes (except peduncle length) showed a different reaction to supplementary and without irrigation conditions, which indicates high genetic diversity among studied genotypes. Regarding most traits, Ter line was the most susceptible genotype to drought in both supplementary and without irrigation conditions. Regarding to GMP and STI indices, Ter, Icajihan and Aghrass lines were the most susceptible genotypes. Cluster analysis in both supplementary and without irrigation conditions classified lines into four groups, but tolerated lines did not get together. In general, according to yield and tolerance indices, Icajihan line was the most suitable genotype for cultivation in non-irrigated conditions. Manuscript profile -
Open Access Article
55 - An Appraisal of Phenotypic Diversity Among Hazelnut Wild Germplasm from Northwest Iran
Ahmad Ershadi Javad Farrokhi Toolir Sona Hossein Ava Thomas Molnar -
Open Access Article
56 - Phenotypic Evaluation and Identification of Superior Persian Walnut (Juglans regia L.) Genotypes in Mazandaran Province, Iran
Mousa Kouhi Ali Rezaei Darab Hassani Saadat Sarikhani Kourosh Vahdati -
Open Access Article
57 - The Effect of Different Water Potentials on Seed Germination and Growth of some Persian Walnut Populations
Leily Heidari Naser Boroomand Mohammad Sadat-Hosseini -
Open Access Article
58 - Morphological and Molecular Evaluation of Persian Walnut Populations in Northern and Western Regions of Iran
R. Karimi A. Ershadi A. Ehtesham Nia M. Sharifani M. Rasouli A. Ebrahimi K. Vahdati -
Open Access Article
59 - Evaluation of Quantitative and Qualitative Characteristics of Persian Walnut (Juglans regia L.) Genotypes in the West of Meshkin-Shahr
Alireza Ghanbari Mohammad Faraji Aliakbar Shokouhian Adel Pyrayesh -
Open Access Article
60 - Evaluating the Resistance of Various Potato Genotypes to Early Blight Disease Caused by Alternaria alternata
NEDA PEYMANI AHMADREZA GOLPARVAR MEHDI NASR-ESFAHANI ESMAEIL MAHMOUDI MAJID SHAMS -
Open Access Article
61 - Evaluation of Genetic Diversity in Soybean (Glycine max (L.) Merrill) Genotypes Based on Agronomic Traits
K. N. MILI B. J. SHIRAZY M. M. MAHBUB -
Open Access Article
62 - Investigation of Genetic Diversity in Some Aloe Sp. Accessions Using Morphological Markers in the in vitro conditions
mansoor saljooghianpour s. mahdi javadzadeh Ahmad EbrahimiFor study the genetic diversity of Aloe germplasm and identify the classification and relationship of germplasm collections and exploiting them in breeding programs, Thirty three Aloe accessions were collected. These accessions were including 31 wild genotypes and 2 com MoreFor study the genetic diversity of Aloe germplasm and identify the classification and relationship of germplasm collections and exploiting them in breeding programs, Thirty three Aloe accessions were collected. These accessions were including 31 wild genotypes and 2 commercial genotypes. Shoot tip explants were washed and sterile. Then, were inoculated on solid MS medium supplemented with 0.5 mgl-1 benzyl adenine + 0.5 mgl-1 α-naphthalene acetic acid and sub-cultured on the same medium for plantlet production and propagation once every four weeks. Plantlets were grown in Completely Randomized Design (CRD) with four replications in greenhouse. After six months, 18 morphological traits were measured and analyzed. Morphological analysis indicated that all the studied various characters have a significant difference at P < 0.01 among Aloe accessions. Also comparison of the means (Duncan’s test) for the genotypes was performed. Maximum amounts for most traits was related to genotype 29 which suggests the potential of this genotype for further studies. The results of orthogonal comparisons between genotypes of wild or native genotypes in a group and the control group showed that the genotypes for most morphological traits studied, there was no significant difference. Cluster analysis indicated that all accessions were divided into three major groups. There were commercial accessions in the third group, and the best wild accession (number of 29) was in this group that is representing high potential of this genotype. Manuscript profile -
Open Access Article
63 - Phylogeny of Central Plateau of Iran Gazelles by Mitochondrial Cytochrome b Gene
راضیه Mohammadi مرتضی NaderiMitochondrial genome sequencing is one of the most used method in the mammalian phylogenetic relationships studies. Such researches mostly focus on intraspecific divergence, prioritization of protective measures, and investigation on the species origin, refugium theory MoreMitochondrial genome sequencing is one of the most used method in the mammalian phylogenetic relationships studies. Such researches mostly focus on intraspecific divergence, prioritization of protective measures, and investigation on the species origin, refugium theory and other studies. Many investigations have been focused on Gazelles in Iran and other parts. Most studies agree on the existence of three species of the Gazella genus, yet there is still much debate about the status of different populations and phylogenetic relationship of the species in different habitats. In this study, we have tried to investigate on the phylogenetic status of the genus in the central plateau populations including Yazd, Isfahan and Kerman. Our results indicated that the populations in the Yazd Province show the most phylogenetic divergence in comparing with Isfahan and Kerman populations. Our results showed that overall genetic diversity of the central plateau is relatively low. With regard to the sample size, we believe that the obtained results should be interpret with caution Manuscript profile -
Open Access Article
64 - The Comparison of Genetic Structure of Zagros tooth-Carp (Aphanius vladykovi( in Gandoman and Shalamzar Wetland in the Chaharmahall-o-Bakhtiari Province by Using Microsatellite Markers
سیده آمنه حسینی علی شعبانی حمیدرضا رضاییZagros tooth carp (Aphanius vladykovi) is a rare and valuable species. This is native fish in Chaharmahal-o-Bakhtiyari province of Iran and lives in springs and wetlands. In this study, to investigate the genetic diversity of this species, sampling was performed from Ga MoreZagros tooth carp (Aphanius vladykovi) is a rare and valuable species. This is native fish in Chaharmahal-o-Bakhtiyari province of Iran and lives in springs and wetlands. In this study, to investigate the genetic diversity of this species, sampling was performed from Gandoman and Shalamzar wetland areas, 23 individuals from each region. 5 pairs microsatellite primer were used to investigate the genetic diversity and showed 100% polymorphism. The average observed heterozygosity was equal to 0.965 that is more than expected heterozygosity (0.867). The mean gene flow and inbreeding coefficient was 23.33 and -0/11, respectively. The result of Fst (%1) and Rst (%14) indicated low genetic differentiation between populations and adequate genetic variation within populations. Analysis of molecular variance indicated %1 differentiation between and % 99 variations within populations.Among the10tests, 5 samples showed following Hardy - Weinberg equilibrium. Manuscript profile -
Open Access Article
65 - Investigating the relationship between gliadin sub-units and grain quality traits in wheat
marouf khalili rehane asadi Mohammad Ali EbrahimiIn order to investigating the relationship between gliadin sub-units and grain quality traits in wheat, 30 varieties and lines of wheat were evaluated in terms of allelic variation at the loci controlling Gliadin protein by Polyacrylamide gradient gel electrophoresis (A MoreIn order to investigating the relationship between gliadin sub-units and grain quality traits in wheat, 30 varieties and lines of wheat were evaluated in terms of allelic variation at the loci controlling Gliadin protein by Polyacrylamide gradient gel electrophoresis (A-PAGE). In this study number of bands observed in the varieties included 1-5 bands for α subunit, 1-5 bands for ß subunit, 3-8 bands for γ and 3-6 bands for ω subunit. Among α subunit allele’s α1 and α3, between ß subunits alleles’ß4, among γ subunit alleles of γ1 and γ5 and between ω subunits alleles of ω5 and ω6 showed the highest percentage of frequency. Percentage of protein with α2 allele, zeleny number with ß3 and ß4 alleles, hardness number with α2 and γ8, and thousand kernel with ω5 and γ5 had positive and significant correlations, while amount of water absorption showed negative and significant correlations whit γ5. Based on the stepwise regression analysis, between alleles α2, ß2 and α5 with protein content, allele ß4 and zeleny number, allele α2 and hardness alleles, γ5 with starch and ω5 with flour water absorption rate and thousand kernels with γ5 alleles statistically significant relationship was found. So the above subcultures can help as biochemical markers for high quality baker's progeny Manuscript profile -
Open Access Article
66 - evaluation genetic diversity to resistant genotypes of drought stress in durum wheat with using SSR molecular markers.
seyed saeed noorinia Shahab SadatTo evaluated of efficiency of selection by markers (MAS). 20 genotypes durum wheat was conducted as a split plot design in randomized complete block are planted and yield components calculated. Also in molecular level of 20 pair starter (SSR) to study genetic diversity MoreTo evaluated of efficiency of selection by markers (MAS). 20 genotypes durum wheat was conducted as a split plot design in randomized complete block are planted and yield components calculated. Also in molecular level of 20 pair starter (SSR) to study genetic diversity between varieties and determine genotypes tolerant to drought stress was used. After extraction of DNA and appointment that quantity and quality polymerase chain reaction took place in the touch down method. In total 58 allele of the 20 locus was detected. The largest allele detected belonged to the Xgwm610, and Xbarc108 with 4 allele and the lowest with 2 allele was reserved markers Xgwm413, Xgdm132, Xwmc48, Xbarc40, Xbarc121, Xgwm375. Average allele produced for each locus in this research is 2/9. Rating and length of the parts produced using the software UVDOC was specified. Similarity matrix, the cluster analysis in UPMMA method and draw dendrogram using NTSYS software took. Standard correlation coefficient of cophentic calculated and the number of 0/86 was obtained. Dendrogram represent 2 cluster of quite distinct. In one cluster the number of genotypes 1, 2, 15, 16, 20, 13, 3, 4, 19, 8, 12, 7, 9, 5, 14 and in another cluster the number of 6, 18, 10, 17, 11 are placed. Genotypes of 5 and 14 of higher stress tolerance index than other varieties. The result showed multiple allele trait a phenomenon quite usual and common in markers (SSR) continuity of trait or could be effect of the multiple genes. Manuscript profile -
Open Access Article
67 - Genetic variation study for agro-morphological traits in safflower genotypes (Carthamus tinctorius L.)
Ali Saremi-Rad Mohammad hadi Taleb Saeid Omrani Khodadad MostafaviGenotype safflower is scattered throughout the world from India and China to Africa and Europe. The aim of this study was to determine the relationship between plant yield, yield components and morphological traits, as well as accurate recognition of traits affecting th MoreGenotype safflower is scattered throughout the world from India and China to Africa and Europe. The aim of this study was to determine the relationship between plant yield, yield components and morphological traits, as well as accurate recognition of traits affecting the yield on 32 safflower genotypes in a randomized complete block design with three replications at the research farm of Islamic Azad University, Karaj Branch. Among the studied genotypes, there was a significant difference in all studied traits such as plant yield, 1000-seed weight, number of branches, number of bolls per plant, boll weight, flowering time, flowering time, plant height and flowering time until there was an examination that indicated a high diversity in the germplasm studied. Among the yield components, the most heritability was attributed to 1000 seed weight and number of branches, and the lowest heritability was attributed to plant yield, which indicates that indirect modification of yield would be more beneficial. Plant height and number of branches can be used as an index of selection in breeding programs. Cluster analysis based on morphological traits categorized genotypes into five clusters. Based on the results of this study, it can be stated that by improving the traits of boll weights and number of bolls per plant, it is possible to achieve the desired grain yield in safflower genotypes. Manuscript profile -
Open Access Article
68 - Genetic evaluation of physiological Traits related to drought tolerance in some bread wheat genotypes under rain-fed conditions
zahra morovati Ezatollah Farshadsfar Mohammad Hosein RomenaAbstract: In order to investigate phenotypic and genotypic correlations between physiological characteristics related to drought tolerance and genetic variation and estimating the genetic parameters of these indices in rain-fed condition, 19 genotypes of bread wheat wer MoreAbstract: In order to investigate phenotypic and genotypic correlations between physiological characteristics related to drought tolerance and genetic variation and estimating the genetic parameters of these indices in rain-fed condition, 19 genotypes of bread wheat were cultivated in a randomized complete block design with three replications in the research field of Agricultural Research Campus Natural Resources of Razi University of Kermanshah, Iran during 2014-15. analysis of variance (ANOVA) showed significant differences for the grain yield (YS), the cell membrane stability )CMS), the relative water loss )RWL(, the leaf water loss (LWL), the relative water protective (RWP), the water use efficiency )WUE( and the leaf greenish value )LGV( under rain-fed condition. The polygon view of GT biplot showed that genotype no. 12 (wc-4931) was the best genotype compared to other genotypes due to YS, WUE, ELWR and LGV. A high positive genetic and phenotypic relation was observed between WUE and Ys, FV/FM and LWL and, ELWR and LGV. In other hand, High heritability and genetic gain were observed for YS, CMS and WUE that reflect the additive gene action. Accordingly, due to the high propotion of additive effects in CMS, YS and WUE, it was suggested that the evaluation of genotypes must be done at erarly generarion of inbreeding. Manuscript profile -
Open Access Article
69 - Evaluation of yield and yield components of some of faba feen (Vicia faba L.) genotypes at different sowing dates in Guilan province
Peyman SharifiThe present experiment was carried out to study the effect of planting date on yield and yield componts of some faba bean genotypes in a split plot based on Complete Randomized Block Design with three replicates in Guilan province during 2014-15 growing season. The main MoreThe present experiment was carried out to study the effect of planting date on yield and yield componts of some faba bean genotypes in a split plot based on Complete Randomized Block Design with three replicates in Guilan province during 2014-15 growing season. The main factor was four planting dates (20 November, 19 December, 5 January and 1 March) and the split factor was six faba bean genotypes (Guilan landrace, Borojerd landrace, France, Barrakat, FILIP3 and FILIP5). The effect of planting date was significant on all of the studied traits. The effect of genotype was also significant on all traits exept of days to maturity and relative water content. Interactions of two factors were significant on seed yield, number of days to emergence, hundred seed weight, number of seeds per pod and number of pods per plant. The highest ripening time of seeds (175.22 days) was observed in the first planting date and this time in the last planting date was reduced by 42%. Due to the significant interaction of genotype by sowing date, the simple effect of genotype was investigated at each level of sowing date and it was shown that in all four sowing dates, Gilan landrace had the highest grain yield with 3208.04, 1858, 110.8, 605.2 kg ha-1, respectively. Delay in planting incrased the seed nitrogen content by 22%. According to the results of present study and due to climatic ondition, early November is considered as an appropriate time for planting faba beans in the studied area. Manuscript profile -
Open Access Article
70 - The study of MHC-DRB3 gene Exon region polymorphisms by PCR-RFLP in some breeds of horses in Iran
Mohammad Tavakoli Alireza Noshary Behzad HemmatiThe major histocompatibility complex (MHC) genes are very important in horse breeding according to relationship with immunity response and resistance or susceptibility to disease. The aim of this research is study of polymorphism possible in exon gene region and populat MoreThe major histocompatibility complex (MHC) genes are very important in horse breeding according to relationship with immunity response and resistance or susceptibility to disease. The aim of this research is study of polymorphism possible in exon gene region and population genetics. To do this, 120 horses were selected randomly from Thoroughbred, Oldenburg, Caspian pony and Arab breeds. Genomic DNA was extracted via blood samples and polymerase chain reaction was done for a 309 bp MHC gene region. The PCR products were digested by RsaI restriction endonuclease for genotyping determination. Results show that there were three allele of A, B and C and six types of genotype of AA, AB, AC, BB, BC and CC with 0.3208, 0.3208 and 0.3584 allele frequency and 0.05, 0.25, 0.2917, 0.1333, 0.125 and 0.15 genotype frequency in all studied samples respectively. The most and least allele frequency in Thoroughbred, Oldenburg, Caspian pony and Arab are related to B, A, A, C and A, B, B, A alleles respectively. The Hardy-Weinberg equilibrium study by X2 and G2 tests shows the equilibrium situation in Oldenburg and Caspian pony breeds and non-equilibrium situation in Thoroughbred and Arab breeds. The overall results shows that the genetic diversity in studied samples can be used as a resource for horse breeding programs. Manuscript profile
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