Evaluation of Genetic Diversity in Some of Canola Cultivars (Brassica napus L.) Using Morphological-agronomic and RAPD Molecular Markers
Subject Areas : Sustainable production technologies
Mansoor
Saljooghianpour
1
(Assistant Professor, Department of Agricultural Engineering, Iranshahr Branch, Islamic Azad University, Iranshahr, Iran)
SeyyedMahdi
Javadzadeh
2
(Assistant Professor, Department of Agricultural Engineering, Iranshahr Branch, Islamic Azad University, Iranshahr, Iran)
Mohsen
Mohseni
3
(Assistant Professor, Department of Agricultural Engineering, Shahid Soleimani Branch, Islamic Azad University, Kerman, Iran)
Keywords: Morphological-agronomic marker, genetic diversity, RAPD molecular marker, Canola,
Abstract :
In order to investigate the genetic diversity of canola cultivars, an experiment was conducted in the form of randomized complete blocks with 9 rapeseed cultivars in 3 replications in Iranshahr region. After culture, DNA extraction was done using CTAB or Dellaporta method with some modifications and 6 pairs of RAPD primers were used for amplification. Also, at the end of the growing season, traits such as plant height, plant fresh weight, plant dry weight, yield and seed oil percentage were measured.The results of the analysis of variance showed that the cultivars have a significant difference at the 1% probability level. Also, there was a positive and significant correlation between the studied traits. Cluster analysis of morphological-agronomical data of rapeseed cultivars in four main groups and cluster analysis of molecular data of rapeseed cultivars in three main groups. Cultivars placed in each group have more genomic similarity and cultivars placed in different groups have more genetic differences. In the analysis of the main coordinates of the molecular data, the first two components could not include all the information obtained, so this information indicates the wide distribution of these markers on the rapeseed genome and can cover a wide area of it. The results of this research showed that according to the diversity observed in the cultivars and based on the results of the grouping of genotypes, it is possible to select genotypes from groups that have a greater genetic distance from each other and use them for specific breeding purposes.
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