The Comparison of Genetic Structure of Zagros tooth-Carp (Aphanius vladykovi( in Gandoman and Shalamzar Wetland in the Chaharmahall-o-Bakhtiari Province by Using Microsatellite Markers
Subject Areas : Journal of Animal Biologyسیده آمنه حسینی 1 , علی شعبانی 2 , حمیدرضا رضایی 3
1 - دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گروه شیلات، گرگان، ایران
2 - دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گروه شیلات، گرگان، ایران
3 - دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گروه محیط زیست، گرگان، ایران
Keywords: genetic diversity, Microsatellite, heterozygosity, Aphaniusvladykovi, Gandoman Wetland,
Abstract :
Zagros tooth carp (Aphanius vladykovi) is a rare and valuable species. This is native fish in Chaharmahal-o-Bakhtiyari province of Iran and lives in springs and wetlands. In this study, to investigate the genetic diversity of this species, sampling was performed from Gandoman and Shalamzar wetland areas, 23 individuals from each region. 5 pairs microsatellite primer were used to investigate the genetic diversity and showed 100% polymorphism. The average observed heterozygosity was equal to 0.965 that is more than expected heterozygosity (0.867). The mean gene flow and inbreeding coefficient was 23.33 and -0/11, respectively. The result of Fst (%1) and Rst (%14) indicated low genetic differentiation between populations and adequate genetic variation within populations. Analysis of molecular variance indicated %1 differentiation between and % 99 variations within populations.Among the10tests, 5 samples showed following Hardy - Weinberg equilibrium.
